40 results about "Isoquercetin" patented technology

The invention provides an isoquercetin and derivative. The structural formula of the isoquercetin and derivative is shown as the formula I, wherein n=1-9, and R is selected from Glu, Rha, Gal, Xyl orAll. The isoquercetin and derivative solves the problem of poor solubility of rutin and quercetin as well as in vivo digestion and absorption of rutin, improves the bioavailability of isoquercetin andpolysaccharide isoquercetin and achieves significant therapeutic effects on prevention and treatment of osteoporosis and hyperlipidemia.

The invention relates to a preparation method for hyperin and isoquercitrin, wherein, the method uses cotton petals as materials and an organic solvent for extraction, and an extracting solution is decompressed and condensed to the extract extractum which is dissolved in proper amount of a water solution, and the column chromatography taking macroporous resins as carriers is made, and the column bed is washed by water until the column bed is colorless, and the column bed is eluted by an ethanol solution, and the eluent is collected and condensed so as to obtain coarse products of the hyperin and isoquercitrin, and purified products of the hyperin and isoquercitrin are obtained by the silica gel or C18 silica gel chromatography of coarse products. The method is low in cost and simple in operation, and provides a new path for acquiring the hyperin and isoquercitrin.

The invention relates to a preparation method and application of a folium apocyni veneti extract, belongs to the field of medicines and aims at increasing the extraction content of quercetin-3-O-beta-D-glucosyl-beta-D-glucoside and quercetin-3-O-beta-D-glucuronide in folium apocyni veneti by 0.5-10 percent and applying the quercetin-3-O-beta-D-glucosyl-beta-D-glucoside and the quercetin-3-O-beta-D-glucuronide to a medicament for treating depression. According to the folium apocyni veneti extract, the total flavonoids content is 58-78 percent, wherein the contained flavonoids component is prepared from the following components in percentage by weight: 5.0-25 percent of the quercetin-3-O-beta-D-glucosyl-beta-D-glucoside, 0.5-10 percent of the quercetin-3-O-beta-D-glucuronide, 0.5-20 percent of rutin, 5.0-25 percent of hyperin, 5.0-20 percent of isoquercetin, 2.0-10 percent of quercetin, 2.0-10 percent of kaempferol and the balance of other flavonoids components. The preparation method and the application are convenient and efficient, are easy in operation and low in cost, can meet development and utilization of the fields of the medicines and foods and have very large application and development values.

The invention relates to a method for preparing a plant extract having the effect of removing dark eye circles. The method is characterized by comprising the following steps: A, respectively selectingfresh sophora japonica and coix seed, washing, drying, pulverizing, sieving, weighing sophora japonica powder and coix seed powder according to the mass ratio of 3:1 as a plant mixture; B, respectively weighing glycerin, isoprene glycol and betaine according to the mass ratio of 4:3:1, carrying out uniform magnetic stirring in a thermostat water bath of 30-50 DEG C, cooling to room temperature asan eutecticevaporate solvent; C, weighing the plant mixture and the eutecticevaporate solvent at a mass ratio of 1:2-6, mixing well, heating to 40-60 DEG C, extracting by ultrasonic vibration for 1-3h, and filtering to obtain the plant extract having the effect of removing dark eye circles. The plant extract having the effect of removing dark eye circles contains various high-content active ingredients such as rutin, isoquercetin, daphnin and coix seed fat, and has a remarkable effect of removing dark eye circles. In addition, the extraction rate of the method is higher.

The invention discloses a health beverage with an efficacy of removing freckle and a production method of the health beverage, belonging to the field of health foods. The health beverage with the efficacy of removing freckle is characterized by being brewed from grapes, Chinese-date powder and roses, wherein the weight ratio of the added grapes to the added Chinese-date powder to the added roses is (50-200):(50-30):(0.5-2). The product contains natural flavonoid substances such as claret polyphenols, resveratrol, melatonin, fruit acid, tannin, Chinese date polyose, quercetin, isoquercetin and the like, has good efficacies of oxidation resistance, free radical removal, skin aging resistance, metabolism acceleration, incitant immunization and the like, is a favorable skin freckle removing product and can ensure the skin to be white, delicate and bright.

The invention provides folium apocyni veneti oral liquid for calming the liver, tranquilizing and enhancing immunity. The folium apocyni veneti oral liquid comprises the following raw materials in part by weight: 10 to 50 parts of folium apocyni veneti, 10 to 20 parts of Eclipta alba, 10 to 20 parts of polygala tenuifolia, 10 to 20 parts of tuber fleeceflower stem, 10 to 20 parts of gardenia, 10 to 20 parts of zizyphus jujube benevolence, 10 to 20 parts of salvia miltiorrhiza, 10 to 20 parts of Ligusticum wallichii, 10 to 20 parts of yanhusuo, 10 to 20 parts of epimedium, 10 to 20 parts of codonopsis pilosula, 10 to 20 parts of poria cocos, 10 to 20 parts of rhizoma zedoariae, 10 to 20 parts of glossy privet fruit, 10 to 20 parts of dogwood, 10 to 20 parts of Concha Margaritiferallsta, 10 to 20 parts of mulberry parasitism, 10 to 20 parts of radix paeoniae alba, 10 to 20 parts of gynostemma, 10 to 20 parts of fructus rosae laevigatae, 10 to 20 parts of astragalus membranaceus, 10 to 20 parts of angelica and 10 to 20 parts of curculigo. The folium apocyni veneti is cold in property, sweet and bitter in taste, and capable of calming the liver, tranquilizing, clearing heat and promoting urination. The folium apocyni veneti oral liquid comprises the main components of rutin, catechin, isoquercetin, glutamic acid, alanine, valine, potassium chloride, and the like, and is capable of generating sperms, supplementing marrow, reinforcing qi, nourishing blood, tonifying brain and tranquilizing.

The invention discloses a method for biosynthesis of quercetin glycoside, which connects the gene of glycosyltransferase UGT73G1 or mutant thereof with sucrose synthase gene to obtain a recombinant plasmid, and constructs a recombinant bacterium containing a double enzyme system; The recombinant strain was inoculated in LB liquid medium, cultured at 25-40 DEG C until OD600 reached 2-3, adding inducer lactose to induce for 12 to 20 hour, centrifuging that fermentation broth to collect bacterial cells, crushing the bacterial cell, and centrifuging to collect supernatant to obtain crude enzyme solution; dissolving quercetin or isoquercetin in DMSO, add crude enzyme solution, sucrose, reaction 8-30 h, adding methanol to stop that reaction, and centrifuging to obtain the supernatant quercetin-3, 4'-Diglucoside; diglucoside. The amino acid sequence of the glycosyltransferase mutant is an amino acid sequence in which the amino acid sequence shown in SEQID NO: 1 is mutated, and the mutated amino acid site is selected from one or more of S151, T168, K274, T293, G361, S365, V371 and F381. The mutant is simple in preparation and high in yield, and realizes the function of quercetin The yieldof 3, 4 '-diglucoside was increased.

The invention discloses aspergillus niger Rha-N1 and an application thereof. An alpha-L-rhamnosidase gene is connected to a pCAMBIA vector to obtain a recombinant plasmid, agrobacterium tumefaciens isintroduced, fermentation expression is performed after aspergillus niger is subjected to agrobacterium tumefaciens mediated transformation, and the enzyme activity of an obtained recombinase is 0.471U/mL. Normal-temperature plasma mutagenesis is performed to obtain a mutant strain with the fermentation broth enzyme activity of 0.652 U/mL. The recombinant aspergillus niger can be applied to hydrolysis of rutin, hesperidin and naringin, finally a substrate is completely converted, and isoquercetin, hesperetin monoglucoside and prunin with the purity larger than 98% are obtained.

The invention relates to a medicament complex which is prepared from total flavone extract of lotus leaf and hypericum japonicum thunb, and new usage for preparing anti-hepatitis B virus medicament. The total flavone of lotus leaf and hypericum japonicum thunb of the invention is total flavone extract which is separated and extracted from lotus leaf and hypericum japonicum thunb which grow in China. The complex of lotus leaf and hypericum japonicum thunb is separated and purified, tested by mass spectrum, 13C and 1HNMR, and proved to contain the following chemical components: quercetin, quercetin-3-O-beta-D-galactopyranose, isoquercetin, and kacmpferol and the like. The complex of total flavone extract is tested by in vitro cell model and in vivo animal model and proved to have strong activity of resisting hepatitis B virus and protecting liver. The complex of total flavone extract can be further prepared into anti-hepatitis B virus medicament and dosage forms can be capsules, tablets, granules, and injection and the like.

The invention discloses a high-temperature resistant flavonoid aglycone invertase and applications thereof. Amino acid sequence of the high-temperature resistant flavonoid aglycone invertase is represented by SEQ ID No.1. Thermal stability of the high-temperature resistant flavonoid aglycone invertase is excellent; the high-temperature resistant flavonoid aglycone invertase is capable of enduring high temperature, and possesses relatively excellent catalytic conversion capability on flavone glycosides of different mother nucleus types and different glucosidic bond types. After a certain time of incubation of the high-temperature resistant flavonoid aglycone invertase with typical flavone glycosides (isoquercetin, astragalin, daidzin, and saprosma ternatum glucoside), it is observed via detection that almost all of the flavone glycosides are converted into corresponding flavonoid aglycones (quercetin, kaempferol, isoflavoues aglycone, and genistein).

The invention relates to a method for preparing an isoquercetin inclusion compound. The method is characterized by comprising the following steps: A, preparing acetylated poloxamer from poloxamer, acetyl chloride and triethylamine in a molar ratio of 1:1:1.2; B, preparing poloxamer-succinate from poloxamer and succinate; C, preparing poloxamer-hydroxypropyl beta-cyclodextrin polymer from hydroxypropyl beta-cyclodextrin and the poloxamer-succinate in a molar ratio of 1:1-1.5; and D, slowly adding an isoquercetin saturated solution into the poloxamer-hydroxypropyl beta-cyclodextrin polymer saturated solution in a volume ratio of 1:1, sufficiently stirring to react for 12-24 hours, adding diethyl ether to separate solid, performing pump-filtering, washing and drying to obtain the isoquercetin inclusion compound. The isoquercetin inclusion compound has the advantages of good water solubility, high encapulation rate and stable product.

The invention discloses a method for simultaneously separating quercetin-3-O-sophoroside, isoquercetin and chlorogenic acid from Poacynum hendersonii leaves. The method comprises a macro-porous resincolumn adsorption process, an eluent stepwise elution process and a gel chromatographic column refining process. The Poacynum hendersonii leaves are used as a raw material, and are processed to obtainPoacynum hendersonii leaf extract, the extract is fully dissolved in distilled water, insoluble substances are filtered out, the obtained filtrate goes through a macro-porous resin column and undergoes stepwise elution with an eluent, and the obtained first-stage eluate is concentrated and freeze-dried to obtain chlorogenic acid having a purity of 52% or above; and the obtained second-stage eluate undergoes reduced pressure distillation, and the obtained product is dissolved in methanol, and is continuously purified by a gel chromatographic column to obtain the quercetin-3-O-sophoroside having a purity of 93% or above and isoquercetin having a purity of 97% or above. The above separation method allows highly pure quercetin-3-O-sophoroside (93% or above), isoquercetin (97% or above ) and chlorogenic acid (52% or above) to be simultaneously obtained in a simple process, so the method has the advantages of simple and feasible process, high production efficiency, high raw material utilization rate, suitableness for industrial production, and high production and practical values.

The invention discloses an ampullaria gigas molluscacide which comprises the following raw materials by mass: 1-20 parts of diatomaceous earth or kaolin, 10-20 parts of tea bran, 30-40 parts of sec butyl acetate, 7-15 parts of dimethyl sulfoxide, 1-15 parts of castor oil polyoxyethylene ether, 3-20 parts of beta-cyclodextrin, 3-8 parts of calcium dodecylbenzenesulfonate, 1-10 parts of sodium lignosulfonate, 5-15 parts of propylene glycol, 1-10 parts of an antifreeze, 1-10 parts of a thickening agent, 1-10 parts of a disintegrating agent and 40-60 parts of a molluscicidal active substance; and the molluscicidal active substance comprises sodium pentachlorophenate, arecoline, ginkgolic acid, gallic acid, tea saponin, isoquercetin and leonurine. The ampullaria gigas molluscacide is prepared by compounding of plant active substances and chemical drugs, the usage of the chemical drugs is reduced, environmental pollution is reduced, by compounding of the plant active substances, the molluscicidal activity of the molluscicide is enhanced, and by compounding with the harmless concentration of sodium pentachlorophenate, the molluscicidal effect is excellent.

The invention provides a method for extracting flos abelmoschus manihot extract. The method comprises the following steps: heating for refluxing and extracting flos abelmoschus manihot for 2 to 3 times by adopting a methanol solution with a concentration of 50 to 80 percent, so as to obtain an extracting solution, wherein each time is lasted for 0.5 to 1 h; decompressing concentrating the extracting solution under a condition that the temperature is lower than 60 DEG C to obtain extract with a proportion of 1.13 to 1.16 g/ml; adding water which is 2 to 4 times the volume of the extract; centrifuging for precipitating, and taking supernatant; taking the centrifuged and precipitated supernatant to pass through a macroporous adsorbent resin column; after eluting for 2 to 3 column volumes by adopting water, then eluting for 3 column volumes by adopting a methanol solution with a concentration of 20 percent; eluting for 1 column volume by adopting a methanol solution with a concentration of50 to 70 percent; concentrating an eluting solution; spray-drying to obtain the extract. The content of total flavonoids in the flos abelmoschus manihot extract obtained by the method provided by theinvention reaches 70 to 90 percent according to a weight percent; meanwhile, effective components including hyperoside, quercetin and isoquercetin can be extracted at the same time; the time is shortand the content is high.

The invention discloses a preparation method of high-activity buckwheat leaf flavonoids. The preparation method comprises the following steps: extracting in 65-75 DEG C water bath by using 75-85% edible ethyl alcohol as a solvent according to a ratio of material to liquid being 1: (30-50), concentrating suction filtration liquid to one eighth to one tenth of the original volume, putting in a high-pressure reaction kettle, hydrolyzing for 20-60 minutes under the vapor pressure of 0.6-0.8Mpa, centrifuging, collecting precipitates, and drying at 60 DEG C, thus obtaining the high-activity buckwheat leaf flavonoid product. The purity of the buckwheat leaf flavonoid product is increased to 90.6% from 73.5% before high-pressure hydrolysis, and the removal ratio of DPPH free radicals is increased by 9.7% compared with the removal ratio before hydrolysis. Due to the adoption of the preparation method, the content of isoquercetin and quercetin which have relatively high biological activity is increased, the content of the isoquercetin is increased to 3.27-20.6% from zero before hydrolysis, and the content of the quercetin is increased to 8.2-22.78% from 2.2% before hydrolysis. The preparation method has the advantages of waste utilization, low cost, no pollution, simple operation and the like.

The invention relates to antioxidative tartary buckwheat and oat liquor which is made from, by weight, tartary buckwheat, oat, sorghum, yeast for making hard liquor, rice chaff, pure water and biological tartary buckwheat flavone class substance. Each part of the biological tartary buckwheat flavone substance is composed of rutin, quercetin, kaempferol, kaempferol-3-rutinoside, quercetin-3-rutinoside, heteroside and isoquercetin. The making method includes the steps that firstly, tartary buckwheat and oat unblended liquor is brewed; then, biological tartary buckwheat and flavone substance is extracted by decocting tartary buckwheat grains and tartary buckwheat roots to be added back to the unblended liquor, and then fermentation and brewing are conducted; thirdly, 60-degree tartary buckwheat and oat unblended liquor is brewed; the unblended liquor obtained in the above steps is blended, filtered and then stored in a stainless steel tank for use; fourthly, laboratory test and measuring, blending or lowering the alcoholic strength and finished product packaging are conducted. The antioxidative tartary buckwheat and oat liquor has obvious functions of lowering blood glucose, lowering lipid, removing free radical and enhancing immunity and the like.

The invention provides a semi-synthesis method of isoquercetin, and solves the problems of relatively high requirements on equipment performance, relatively low yield and content of isoquercetin and difficulty in purification in the existing pressurization preparation of isoquercetin. The semi-synthesis method of isoquercetin is characterized by comprising the following steps of 1) placing rutin in a reactor, adding water, ethyl acetate and liquid rhamnosidase, and carrying out an enzyme hydrolysis reaction to obtain a reaction solution; 2) standing and layering the reaction solution obtainedin the step 1), and collecting an ethyl acetate phase; 3) decolorizing the ethyl acetate phase obtained in the step 2); and 4) concentrating the ethyl acetate phase decolored in the step 3), standingfor cooling crystallization, and carrying out suction filtration to obtain the high-purity isoquercetin.

The invention discloses an application of an optimized arabidopis thaliana glycosyl transferase gene in the preparation of isoquercitrin, and the nucleotide sequence of the gene is represented by the SEQ No.3. The provided glycosyl transferase has high specificity, and is capable of specifically converting quercetin into isoquercitrin. The provided isoquercitrin production method is simple and the purity of obtained isoquercitrin is high.

The invention discloses a high efficiency soil modifying agent, and a preparation method and applications thereof. The high efficiency soil modifying agent comprises, by weight, 15 to 30 parts of persimmon skin powder, 5 to 12 parts of riboflavin, 6 to 10 parts of lappaconitine, 6 to 10 parts of isoquercetin, 5 to 8 parts of niclosamide, and 4 to 10 parts ofisomaltose hypgather. The high-efficiency soil improving agent is excellent in treatment effect, less in raw materials, less in application amount, high in treatment efficiency, simple in preparation process, and is convenient for production.

The invention belongs to the technical field of medicine and health care products, and discloses a composition for preventing or treating non-alcoholic fatty liver disease and obesity and a preparation method and application thereof, the composition comprises isoquercetin and vitamin C, and the mass ratio of isoquercetin to vitamin C is (0.1-2):1. According to the composition, the isoquercetin and the vitamin C are compounded according to a specific proportion, the vitamin C promotes fat metabolism and accelerates lipolysis, the isoquercetin can rapidly oxidize fatty acid and can reduce lipid generation, the isoquercetin and the vitamin C can achieve a synergistic effect, and the curative effect on obesity and non-alcoholic fatty liver is obvious.