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Method for preparing hyperoside and isoquercetin from cotton petal

A technology of hyperoside and isoquercetin, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant raw materials, etc.

Inactive Publication Date: 2008-09-10
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above patents for the preparation of hyperin and isoquercetin failed to make full use of my country's rich cotton flower resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] a. Using cotton petals as raw material, crush the dried cotton petals into coarse powder, add 8 times of 70% ethanol to heat and reflux to extract twice, each time for 2 hours, combine the extracts, and concentrate to obtain the extract;

[0024] b. Concentrate the extract to extract under reduced pressure, add water to dissolve, then put the aqueous solution on the AB-8 macroporous resin column, and wash the column bed with water until it is colorless;

[0025] c. Then wash 5 column volumes with 30% ethanol, collect the eluate, and concentrate to obtain crude products of hyperin and isoquercetin;

[0026] d. Dissolve the crude product in ethyl acetate, apply silica gel column chromatography, and elute with petroleum ether-ethyl acetate-methanol-acetic acid solution to obtain pure products of hyperin and isoquercetin.

Embodiment 2

[0028] a. Using cotton petals as raw material, crush the dried cotton petals into coarse powder, add 15 times of methanol, perform ultrasonic extraction for 3 times, each time for 1 hour, and combine the extracts;

[0029] b. Concentrate the extract to an extract under reduced pressure, add water to dissolve, then put the aqueous solution on a D101 macroporous resin column, and wash the column bed with water until it is colorless;

[0030] c. Then wash 4 column volumes with 50% ethanol solution, collect the eluate, and concentrate to obtain crude products of hyperin and isoquercetin;

[0031] d. After dissolving the crude product with aqueous methanol, apply C18 silica gel column chromatography and elute with 90% aqueous methanol to obtain pure hyperin and isoquercetin.

Embodiment 3

[0033] a. Using cotton petals as raw material, crush the dried cotton petals into coarse powder, add 20 times of acetone, heat and microwave-assisted extraction for 3 times, each time for 30 minutes, and combine the extracts;

[0034] b. Then concentrate the extract under reduced pressure to extract, add water to dissolve, then put the aqueous solution on the NKA-9 macroporous resin column, and wash the column bed with water until it is colorless;

[0035] c. Then wash 3 column volumes with 40% ethanol, collect the eluate, concentrate, and obtain crude products of hyperin and isoquercetin;

[0036] d. Dissolve the crude product in ethyl acetate, apply C18 silica gel column chromatography, and elute with 80% aqueous methanol to obtain pure hyperin and isoquercetin.

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PUM

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Abstract

The invention relates to a preparation method for hyperin and isoquercitrin, wherein, the method uses cotton petals as materials and an organic solvent for extraction, and an extracting solution is decompressed and condensed to the extract extractum which is dissolved in proper amount of a water solution, and the column chromatography taking macroporous resins as carriers is made, and the column bed is washed by water until the column bed is colorless, and the column bed is eluted by an ethanol solution, and the eluent is collected and condensed so as to obtain coarse products of the hyperin and isoquercitrin, and purified products of the hyperin and isoquercitrin are obtained by the silica gel or C18 silica gel chromatography of coarse products. The method is low in cost and simple in operation, and provides a new path for acquiring the hyperin and isoquercitrin.

Description

technical field [0001] The invention belongs to the technical field of preparation of active ingredients of medicines, in particular to a preparation method of active ingredients of hyperin and isoquercetin. Background technique [0002] Recent studies have shown that: Hyperin (Hyerin) and isoquercetin (isoquercetrin), which belong to flavonoid glycosides, are widely present in various plants. At present, the most studied and prominent physiological activities and pharmacological effects are good analgesic effect and protective effect on cardiovascular and cerebrovascular. It has anti-myocardial ischemic injury, anti-myocardial lipid peroxidation and other effects. Hyperin has obvious local analgesic effect, externally used clinically, it can inhibit the discharge of peripheral nerves induced by various pain-causing factors; hyperin has a significant protective effect on cerebral ischemia-reperfusion injury in mouse cerebral ischemia model , hyperoside also has the effect o...

Claims

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Application Information

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IPC IPC(8): C07H17/07C07D311/30A61K36/185
Inventor 吴韬阿吉艾克拜尔・艾萨热依木古丽・阿布都拉赵永昕
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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