35 results about "Hyperoside" patented technology

The invention discloses an on-line extraction self-optimization method for acanthopanax sessiliflorus flavone based on reinforcement learning. According to the method, multi-source data in the extraction process is collected in real time, and a fusion feature vector is generated after preprocessing; establishing a mapping relation from the feature vector to the contents of rutin, quercetin, kaempferol and hyperoside by using an offline test sample, and constructing a virtual measurer capable of outputting a target flavone composition vector and uncertainty thereof; constructing a safety domain boundary according to a solvent flash point temperature, an equipment tolerance temperature upper limit and a component thermal degradation temperature threshold, and calculating a time-varying safety constraint threshold in combination with uncertainty; performing linkage optimization on the temperature, the ethanol volume fraction, the solid-to-liquid ratio, the soaking time and the circulating flow by adopting a reinforcement learning strategy, and comprehensively considering flavone composition deviation and solvent energy consumption by a reward function; and finally, outputting the optimized parameters to extraction equipment for execution. According to the method, online, lossless and self-adaptive process optimization control is realized, inter-batch fluctuation is remarkably reduced, and the resource utilization efficiency is improved.

The invention relates to a spleen-tonifying, appetite-stimulating and digestion-promoting traditional Chinese medicine preparation UPLC detection method and a fingerprint spectrum, and belongs to the technical field of medicine detection. The invention aims to solve the technical problems that the existing quality control method of the spleen-tonifying, appetite-promoting and digestion-promoting pills lacks comprehensive and objective analysis on the spleen-tonifying, appetite-promoting and digestion-promoting pills, and the problems of low precision, poor reproducibility, low efficiency and the like during detection of related components in the prior art are solved. According to the technical scheme, the UPLC detection method is characterized in that a mixed standard substance of gallic acid, 5-hydroxymethylfurfural, chlorogenic acid, sinapine thiocyanate, sinapic acid, hyperoside, 3, 6-dimustard acyl sucrose, hesperidin, naringenin, nobiletin and hesperetin is taken as a contrast, an acetonitrile-0. 1% formic acid aqueous solution is taken as a mobile phase, gradient elution is carried out, and the content of chlorogenic acid in the sample is detected. The qualitative and quantitative detection of the effective components of the spleen-tonifying, appetite-promoting and digestion-promoting pill is realized.

The invention relates to the field of traditional Chinese medicine quality detection, in particular to a method for detecting the content of chemical components in cotton flower medicinal materials. The detection method comprises the following steps: providing a reference substance solution, wherein a reference substance comprises one or more of hyperoside, isoquercitrin, astragalus smicus glycoside, quercetin, quercetin-7-O-glucoside and quercetin-3 '-glucoside; preparing a test solution by using a cotton flower medicinal material sample to be detected; and detecting the reference substance solution and the test solution by high performance liquid chromatography, and determining the content of chemical components corresponding to the reference substance in the to-be-detected cotton flower medicinal material sample, the high performance liquid chromatography meets one or more of the following conditions: a stationary phase is a C18 chromatographic column; the mobile phase comprises a mobile phase A, a mobile phase B and a mobile phase C, the mobile phase A comprises methanol, the mobile phase B comprises acetonitrile, and the mobile phase C comprises a phosphoric acid aqueous solution. The whole method is high in sensitivity, simple and convenient to operate, relatively low in cost and short in detection time.

The invention relates to application of combination of hyperoside and salvianolic acid B to preparation of a medicine for treating NAFLD, and belongs to the technical field of biological medicine. The hyperoside and the salvianolic acid B have a synergistic effect in preparation of the medicine for treating the non-alcoholic fatty liver disease when used in combination according to the required treatment amount, the curative effect can be enhanced and the side effect can be reduced through combined use of the two medicines, the effect is better than that of independent medication, a new thought is provided for treating the non-alcoholic fatty liver disease, and the application prospect is wide. The method has important significance and wide application prospects.

The invention discloses an acanthopanax endophytic fungus DLCWJ05 for producing hyperoside and acacetin and application of the acanthopanax endophytic fungus DLCWJ05, relates to the field of microorganisms, and aims at solving the problem that the consumption of plant resources is large when hyperoside and acacetin are obtained by an existing method. The endophytic fungi DLCWJ05 of the acanthopanax senticosus are alternaria alternata DLCWJ05 and are preserved in the China Center for Type Culture Collection on July 11, 2025, and the preservation number of the endophytic fungi DLCWJ05 is CCTCC NO: M 20251589. The endophytic fungi DLCWJ05 of the acanthopanax senticosus can be fermented to produce hyperoside and acacetin, and also has antioxidant activity. And a novel method is provided for producing the acanthopanax secondary metabolite by adopting a microbial fermentation method.

The present application relates to the application of hyperoside and salvianolic acid B in the preparation of a drug for treating non-alcoholic fatty liver disease, and belongs to the technical field of biological medicine. The hyperoside and salvianolic acid B have a synergistic effect in the preparation of a drug for treating non-alcoholic fatty liver disease when used in combination in a required amount, and the combination of the two drugs can enhance the curative effect and reduce side effects, which is more effective than the use of a single drug, provides a new idea for treating non-alcoholic fatty liver disease, and has important significance and wide application prospect.

The invention discloses a fingerprint detection method for quality control of Huanglong cough and asthma capsules / Huanglong cough granules, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, a high performance liquid chromatography-mass spectrometry technology is adopted, 5-hydroxymethylfurfural, vitexin, hyperoside, irisflorentin, epimedin B, icariin, irisflorentin, sub-irisflorentin and baohuoside I are taken as reference substances, and different batches of test samples are detected by optimizing extraction conditions and chromatographic parameters, so that the fingerprint spectrum is successfully constructed. The method is simple and easy to implement, high in precision and good in reproducibility, multi-component synchronous identification can be achieved at a time, the detection efficiency is greatly improved, and time and cost are saved; the quality of a plurality of characteristic components of the Huanglong cough and asthma capsule / Huanglong cough granules can be accurately, quickly and simultaneously monitored, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect.

The present application relates to the technical field of fruit quality improvement, and particularly relates to application of hyperoside in improving grape fruit hardness. The scheme provided in the present application is verified by a large number of field tests, after spraying the plant with hyperoside solution, the fruit hardness of four grape varieties of'red double taste', 'rose flavor','sunshine rose' and 'italy' is all significantly improved compared with the control group, wherein the hardness of'red double taste' and 'rose flavor' is greatly improved, the hardness is increased by about 20%~25% in the grape ripening period, the hardness of'sunshine rose' and 'italy' is relatively small, the fruit hardness is increased by about 8%~16% in the grape color softening period; in addition to improving the fruit hardness, spraying the hyperoside solution can also promote the color uniformity of the grape fruit of some varieties, increase the soluble solids content, reduce the titratable acid content, and make the fruit flavor better; the method is compatible with the conventional grape planting management measures, is easy to popularize and apply in the grape planting area, and can bring significant economic benefits to the majority of growers.

The present invention discloses a quality detection method for Wumeizhili granules, belonging to the technical field of quality detection of traditional Chinese medicines. The method includes characteristic spectrum identification, and specifically comprises the following steps: (1) preparing a reference solution of hyperoside, a reference solution of coumarin, a reference solution of codonopsis pilosula, a reference solution of cinnamic acid, a reference solution of psoralen, a reference solution of isopsoralen, and a reference solution of limonin; (2) preparing a test solution using Wumeizhili granules; and (3) performing liquid chromatography detection on the seven reference solutions and the test solution. The quality detection method of the present invention has high accuracy, strong specificity, and reliable detection results, achieving effective quality control of Wumeizhili granules, meeting the needs of modern production, and having important significance for quality detection and clinical use of traditional Chinese medicines.

The invention discloses application of hyperoside to treatment of endometriosis, and aims to evaluate whether hyperoside has an anti-fibrosis effect when applied to a rat endometriosis model by taking fibrosis as a treatment target. The method comprises the following steps: constructing a rat endometriosis model through an autotransplantation method, dividing molded rats into a low-dose group, a medium-dose group, a high-dose group, a model group and a western medicine control group 4 weeks after successful modeling, and laparotomizing and taking out ectopic lesions after respectively taking medicines for 4 weeks. HE staining and masson staining methods are used for mutually proving the fibrosis degree, the expression conditions of fibrosis indexes a-SMA, Co-1, CTGF and Fn are detected in an immunohistochemical mode, and the anti-fibrosis effect of hyperoside is evaluated. As a result, hyperoside can prevent the development of fibrosis in endometriosis, and thus hyperoside can be used as a drug for treating or preventing fibrosis in endometriosis.

This invention provides a green preparation method for hyperoside derived from lotus leaves and its application, belonging to the field of natural product separation and purification technology. Addressing the shortcomings of existing preparation methods, this invention, for the first time, uses abundant and inexpensive lotus leaves and their extracts as raw materials, employing green solvents to establish a green, efficient, high-purity, simple, rapid, and scalable method for preparing hyperoside. This provides raw materials for the development of hyperoside series products and therefore has significant practical application value.

The application discloses a quality detection method of a Yishen Qingli Huoxue recipe, which comprises preparation of a test sample solution, preparation of a standard solution, preparation of a standard curve and content determination. The application screens an optimal test sample preparation method, a preparation method of a control sample and optimal chromatographic conditions and mass spectrometric analysis conditions through a large number of experiments, and establishes a content determination method of the Yishen Qingli Huoxue recipe, which can simultaneously determine the contents of 11 compounds with different structural types, such as resveratrol, rhein, formononetin, aloe-emodin, emodin, chlorogenic acid, polydatin, astilbin, hyperoside, calycosin-7-glucoside and beta-ecdysterone. The method is favorable for comprehensively monitoring the quality of the Yishen Qingli Huoxue recipe and guaranteeing clinical curative effects. Moreover, the method has the advantages of good stability, high accuracy, good repeatability and high precision.

The application discloses a use of hyperoside in resisting equid herpesvirus 8 (EHV-8) infection. Through qPCR, Western Blot and other experimental techniques, it is revealed on multiple levels that the hyperoside can significantly inhibit the replication of EHV-8, and the mechanism of the hyperoside in resisting the EHV-8 infection is clarified from the aspect of blocking the internalization of the EHV-8, so that the hyperoside can be applied in preparing an anti-EHV-8 medicine.

The invention belongs to the field of gene engineering, and relates to a gene combination for synthesizing homoeriodictyol, a recombinant plasmid combination, a strain and a method for heterologous biosynthesis of homoeriodictyol. The gene combination comprises the following genes: a flavonoid 3 '5'-hydroxylase gene EfF3 '5' H-Unigene 37679, a flavonoid 3 '5'-hydroxylase gene EfF3 '5' H-Unigene 37679, a flavonoid 3 '5' The flavonoid 3 '-hydroxylase genes are EfF3' H-CL3877-2 and EfF3 'H-CL13142-2, and the flavonoid 3'-hydroxylase genes are EfF3 'H-CL3877-2 and EfF3' The flavonoid 3 '-hydroxylase gene EfFOMT-Unigene 24812 has the structural formula shown in the specification. The invention provides a potential method for replacing traditional artificial cultivation or submerged fermentation acquisition, and lays a foundation for further transforming efficient biosynthesis through metabolic engineering.

The invention discloses a medicine detection method, which can simultaneously determine the contents of beta-asarone, schizandrin, hyperoside, verbenin, morinda officinalis verbenin, glycyrrhizic acid and other components in a medicine, and can be used for quality control of the medicine.

The invention discloses glycosyl transferase IlUGT88A3 and application of the glycosyl transferase IlUGT88A3 in flavonoid glycoside synthesis, and belongs to the technical field of gene engineering and enzyme engineering. The glycosyl transferase IlUGT88A3 can be used for specifically catalyzing hydroxyl at C3 site of quercetin to generate glucosylation and galactosylation, so that the isoquercitrin and the hyperoside are efficiently synthesized. The optimum pH is 7.0-7.5, the optimum temperature is 30 DEG C, and the reaction conditions are mild; the method has good substrate heterogeneity, and can be used for carrying out site-specific glycosylation modification on various flavonoid receptors by utilizing sugar donors such as UDP-glucose, UDP-galactose, UDP-rhamnose, UDP-xylose and the like. According to the method, the blank of shortage of flavonoid 3-O-galactosyl transferase resources is filled, the catalytic specificity is high, the efficiency is high, the product is pure, the method can be used for in-vitro enzymatic synthesis and plant heterologous synthesis of high-value flavonoid glycosides such as hyperoside, and a core enzyme element and a technical support are provided for green and efficient preparation of medical raw materials.

The application discloses application of 2''-O-galloylgeniposide in preparation of anti-fibrosis drugs. In the application, the 2''-O-galloylgeniposide can inhibit up-regulation of cell alpha-smooth muscle actin (alpha-SMA) and / or fibronectin expression and up-regulation of type I collagen (CO11A1) gene, fibronectin (FN1) gene and / or alpha-smooth muscle actin (ACTA2) gene transcription in a lung epithelial cell interstitial transformation model, a skin fibroblast activation model, a liver stellate cell activation model and a bleomycin-induced mouse lung fibrosis animal model, and the 2''-O-galloylgeniposide shows a significant anti-fibrosis effect and can be used for preparation of anti-fibrosis drugs.

This invention discloses a method for increasing the content of flavonoids and cyanidin in *Phyllostachys edulis*, belonging to the field of plant cultivation technology. This invention uses continuous irradiation of *Phyllostachys edulis* with light of specific wavelengths and intensities; light with wavelengths of 354-478 nm is the main wavelength inducing culm discoloration; when the blue light intensity exceeds 8 μmol / m², the irradiation is further enhanced. 2 At a certain temperature, the color and anthocyanin content of bamboo increased significantly. After light treatment, the contents of six flavonoids, including vitexin, isohypericin, narcissin, hyperoside, luteolinin, and luteolin, increased by 9.02, 8.79, 2.34, 8.5, 9.4, and 1.9 times, respectively; the content of cyanidin-3-O-rutin increased by 7.41 times.

The invention relates to the technical field of fruit quality improvement, in particular to application of hyperoside in improvement of grape fruit hardness. According to the scheme provided by the invention, a large number of field trials verify that after the hyperoside solution is sprayed to plants, the fruit hardness of the four grape varieties of 'Red double flavor', 'Rosa rugosa', 'Sunshine Rosa' and 'Italian' is remarkably improved compared with that of a control group, the hardness of the 'Red double flavor' and the 'Rosa rugosa' is greatly improved, the hardness is improved by about 20%-25% in the grape mature period, and the fruit hardness is improved by about 20%-25% in the grape mature period. The hardness of 'Sunrose' and 'Italian' is relatively slightly improved, and the fruit hardness is improved by about 8-16% in the color-changing softening period of grapes; besides, the fruit hardness is improved, and by spraying the hyperoside solution, the color uniformity of partial varieties of grape fruits can be promoted, the soluble solid content is improved, the titratable acid content is reduced, and the fruit flavor is better; the method has good compatibility with conventional grape planting management measures, is easy to popularize and apply in grape planting production areas, and can bring remarkable economic benefits to vast farmer households.

The invention discloses a culture solution of Vdelta2T cells as well as a culture method and application of the culture solution. The culture solution comprises an activation culture medium and an amplification culture medium, and a serum-free culture medium is taken as a basic solution; calcium chloride, IL-2, IL-15, IL-21, zoledronic acid, L-glutamine, beta-mercaptoethanol, N-acetylcysteine, human AB serum and silibinin are added into the serum-free culture medium of the activated culture medium; iL-2, human AB serum and hyperoside are added and contained in a serum-free culture medium of the amplification culture medium. According to the culture solution of the Vdelta2T cells and the multiplication culture method for culturing the Vdelta2T cells by using the culture solution, the in-vitro amplification capacity (the amplification multiple is as high as 7416 times) of the Vdelta2T cells can be remarkably improved, the purity of amplified NK cells is as high as 99%, and the tumor cell killing power (the tumor killing effect is relatively good when the ratio of E to T is 1: 1) can be remarkably improved.

This invention provides a functional composition and its application, belonging to the field of functional food technology. The functional composition of this invention comprises the following components in parts by weight: 10-25 parts of Sichuan pepper extract, 5-10 parts of Gastrodia elata extract, and 2-5 parts of saffron extract; wherein the Sichuan pepper extract contains 60%-80% total flavonoids by weight, and the total flavonoids include hyperoside at 5%-15% by weight of the Sichuan pepper extract. The functional composition of this invention not only shortens sleep latency, but more importantly, it also reduces the frequency of nighttime awakenings, effectively reducing the number of nighttime awakenings at the root cause.

The application discloses a characteristic chromatogram construction method and identification method of Senecae Scandentae, its decoction pieces, standard decoction and formula granules, and relates to the technical field of traditional Chinese medicine identification. The characteristic chromatogram construction method comprises the following steps: methanol is used to extract the to-be-detected sample to obtain a test sample solution; methanol is used to dissolve 5-caffeoylquinic acid, chlorogenic acid, caffeic acid, 4-caffeoylquinic acid, rutin, hyperoside, isoquercitrin and 4,5-dicaffeoylquinic acid as a control sample solution; and a liquid chromatograph is used for determination. The chromatographic column of the liquid chromatograph uses phenyl-methyl polysiloxane as a stationary phase, acetonitrile as a mobile phase A and 0.05vol%-0.3vol% methanoic acid aqueous solution as a mobile phase B for gradient elution. The characteristic chromatogram constructed by the application is rich in information and can quickly and comprehensively reflect the quality of Senecae Scandentae, its decoction pieces, standard decoction and formula granules.

The invention relates to the technical field of diarrhea treatment, in particular to application of hyperoside in preparation of a medicine for treating compound diarrhea. The hyperoside provided by the invention has the effects of simultaneously covering secretion inhibition, anti-inflammatory repair and visceral pain relief, and can be used for efficiently treating compound diarrhea.

The invention discloses a thin-layer chromatography detection method, quality control and evaluation of pogonatherum crinitum. The thin-layer chromatography detection method comprises the following steps: providing a pogonatherum crinitum test solution; respectively providing reference substance solutions of hyperoside, quercetin, astragalus smicus glycoside and kaempferol; and dripping the test solution and the reference solution on the same thin layer chromatography plate, developing by using a mixed solution of trichloromethane, methanol and formic acid in a volume ratio of (8-12): (1-2.2): (1-2.2) as a developing solvent, taking out, airing, developing, and inspecting. According to the method, rapid separation and identification of hyperoside, quercetin, astragalin and kaempferol in the pogonatherum crinitum are achieved, thin-layer chromatography spots are clear, the accuracy and comprehensiveness of pogonatherum crinitum quality control are improved, and establishment and overall evaluation of pogonatherum crinitum medicinal material quality standards are facilitated.

The invention discloses an application of a pyracantha fortuneana fruit extract in prevention and treatment of ulcerative colitis, and relates to the technical field of food medicines. The main components of the pyracantha fortuneana fruit extract comprise total flavonoids, total polyphenols and total triterpenes. Wherein the total flavonoids and the total polyphenols comprise hyperoside, rutin, isoquercitrin and epicatechin, and the total triterpenes comprise jujubogenin and the like. Functional food, health care products, medicines and other products prepared from the pyracantha fortuneana fruit extract can reduce the disease activity index, increase the colon length, reduce the abnormally-increased pro-inflammatory cytokine TNF-alpha content in serum, improve the glutathione peroxidase activity and achieve prevention and treatment of ulcerative colitis. Therefore, the pyracantha fortuneana fruit extract disclosed by the invention can be used as a raw material for preparing foods and medicines for preventing and treating ulcerative colitis.

The invention discloses a method for detecting the content of hyperoside in a humifuse euphorbia herb sample. The detection method comprises the following steps: preparing a humifuse euphorbia herb sample test solution and a hyperoside reference substance solution; respectively detecting the reference substance solution and the test substance solution by using an ultra-performance liquid chromatograph, and comparing the maps of the reference substance solution and the test substance solution; a mobile phase used in the detection is a methanol-acetonitrile-0. 1% formic acid solution; and calculating the content of hyperoside in the humifuse euphorbia herb sample by utilizing a regression equation of the sample size and the peak area of the components. The quality detection method of the humifuse euphorbia herb sample provided by the invention is simple to operate, good in reproducibility and stability, high in accuracy, reliable in recovery rate and high in detection efficiency, and provides a basis for standardizing the market and reasonable development of humifuse euphorbia herb medicinal materials and preparations.

The application discloses a method for detecting the content of multiple components in persimmon calyx samples and application thereof. The detection method comprises the following steps: taking persimmon calyx sample powder, precisely weighing, adding an extraction solvent, performing extraction, centrifugation, taking supernatant, evaporating to dryness, constant volume, filtration, taking the filtrate, and obtaining a test sample solution; the mixed control sample solution and the test sample solution are respectively injected into an ultra-high performance liquid chromatograph, and the characteristic maps of the control sample solution and the test sample solution are compared; the content of the components in the persimmon calyx sample is calculated by using the regression equation of the sample injection amount and the peak area; the components are hyperoside, isoquercitrin, 2"-O-galloyl hyperoside, astragalin, quercetin or kaempferol. The quality detection means of the persimmon calyx sample provided by the application can simultaneously determine the content of six components, is simple in operation, good in reproducibility and stability, high in accuracy, reliable in recovery rate, and high in detection efficiency, and provides a basis for standardizing the persimmon calyx medicinal material and preparation market and reasonably developing.

The invention provides a preparation method of an apocynum venetum antihypertensive lozenge, which comprises the following steps: (1) pretreatment of apocynum venetum leaves: withering fresh apocynum venetum leaves, performing fixation, rolling and drying in the sun, and keeping the water content to be less than or equal to 8% to obtain dry apocynum venetum tea; (2) preparation of an apocynum venetum extract: carrying out warm immersion extraction on dried apocynum venetum tea, collecting filtrate, and carrying out vacuum concentration and drying to obtain dry paste; and (3) tabletting the apocynum venetum: uniformly mixing the dry paste with non-dairy creamer and D-mannitol, granulating, adding a lubricant, and tabletting to obtain the apocynum venetum buccal tablet. According to the apocynum venetum lozenge disclosed by the invention, the quick release of effective components in a body is ensured, the effective absorption of the effective components is ensured, the content of total flavonoids per 100g is 3.4 g or above averagely, and the content of hyperoside per 100g is 43mg or above; the lozenge is convenient to prepare, transport, carry and store; the traditional Chinese medicine composition is good in taste, convenient to take and clinically use, attractive in appearance, easier to accept by patients and also suitable for market requirements.

The application belongs to the field of medicine and particularly relates to a eucommia extract composition, a preparation method and application. Components according to weight fractions include the following components: eucommia polysaccharide 5-10 parts, rutin 5-15 parts, hyperoside 5-15 parts, isochlorogenic acid C 1-5 parts, apigenin 1-5 parts and oleanolic acid 1-5 parts. A preparation method mixes eucommia polysaccharide, rutin, hyperoside, isochlorogenic acid C, apigenin and oleanolic acid or adds a solvent to mix to obtain the composition. The composition has a significant synergistic effect on the treatment of membranous nephropathy, thereby providing a new idea for the prevention and / or treatment of membranous nephropathy related diseases, the preparation method is simple, suitable for large-scale production and popularization and use, and the cost is reduced.

Provided are a product for treating Helicobacter pylori-negative chronic atrophic gastritis with intestinal metaplasia and / or with dysplasia, for reversing the pathology of gastric precancerous lesions, and for preventing the occurrence of gastric cancer, and a treatment method therefor. The product is a lotus leaf extract, and representative active ingredients thereof include, but are not limited to, isoquercetin, quercetin-3-O-glucuronide, hypericin, and nuciferine. The use comprises at least one of treating Helicobacter pylori-negative chronic atrophic gastritis with intestinal metaplasia and / or with dysplasia, reversing gastric precancerous lesions, and preventing the occurrence of gastric cancer. In vitro experiments show that the lotus leaf extract can inhibit malignant proliferation of gastric precancerous lesion epithelial cells and reduce the active oxygen level thereof. In vivo experiments show that the lotus leaf extract can effectively improve the pathology of the Helicobacter pylori-negative gastric precancerous lesions and prevent the occurrence of gastric cancer in animal models. Clinical trials have shown that the lotus leaf extract can effectively treat the pathological conditions and clinical symptoms in patients with the Helicobacter pylori-negative chronic atrophic gastritis with intestinal metaplasia and / or with dysplasia.