52 results about "Hyperoside" patented technology

The invention relates to the technical field of dental restoration materials, in particular to a bacteriostatic dental restoration composite film and a preparation method thereof. The oral cavity restoration composite film is composed of an upper-layer antibacterial film, a middle-layer polycaprolactone fiber film and a lower-layer bone restoration film. The upper-layer antibacterial film is prepared from the following components in parts by weight: 3 to 4 parts of cinnamon essential oil, 0.5 to 1 part of xanthan gum, 0.5 to 1 part of seed meal protein, 0.1 to 0.15 part of tannic acid, 5 to 7 parts of silk fibroin, 1 to 1.5 parts of polylactic acid, 0.6 to 1 part of chitosan, 1 to 1.5 parts of polyvinyl alcohol and 1.5 to 2.5 parts of glycerol; the lower-layer bone repair membrane is prepared from the following raw materials in parts by weight: 2 to 3 parts of nano-hydroxyapatite, 3 to 5 parts of citric acid, 6 to 9 parts of lactic acid, 3 to 4 parts of tyrosine, 1 to 2 parts of glycollic acid, 2 to 3 parts of casein, 0.2 to 0.3 part of hyperoside and 1 to 2 parts of gelatin.

The invention discloses a construction method and an identification method of characteristic chromatograms of a senecio scandens medicinal material as well as decoction pieces, standard decoction and formula granules thereof, and relates to the technical field of traditional Chinese medicine identification. The construction method of the specific chromatogram comprises the following steps: extracting a to-be-detected sample with methanol to obtain a test solution; the method comprises the following steps: dissolving 5-caffeoylquinic acid, chlorogenic acid, caffeic acid, 4-caffeoylquinic acid, rutin, hyperoside, isoquercitrin and 4, 5-dicaffeoylquinic acid with methanol to obtain a reference solution; and a liquid chromatograph is adopted for determination. A chromatographic column of the liquid chromatograph takes phenyl-methyl polysiloxane as a stationary phase, acetonitrile as a mobile phase A and 0.05 vol%-0.3 vol% formic acid aqueous solution as a mobile phase B for gradient elution. The information of the characteristic chromatogram constructed by the method is rich, and the quality of the senecio scandens medicinal material, decoction pieces, standard decoction and formula granules can be quickly and comprehensively reflected.

The invention discloses an on-line extraction self-optimization method for acanthopanax sessiliflorus flavone based on reinforcement learning. According to the method, multi-source data in the extraction process is collected in real time, and a fusion feature vector is generated after preprocessing; establishing a mapping relation from the feature vector to the contents of rutin, quercetin, kaempferol and hyperoside by using an offline test sample, and constructing a virtual measurer capable of outputting a target flavone composition vector and uncertainty thereof; constructing a safety domain boundary according to a solvent flash point temperature, an equipment tolerance temperature upper limit and a component thermal degradation temperature threshold, and calculating a time-varying safety constraint threshold in combination with uncertainty; performing linkage optimization on the temperature, the ethanol volume fraction, the solid-to-liquid ratio, the soaking time and the circulating flow by adopting a reinforcement learning strategy, and comprehensively considering flavone composition deviation and solvent energy consumption by a reward function; and finally, outputting the optimized parameters to extraction equipment for execution. According to the method, online, lossless and self-adaptive process optimization control is realized, inter-batch fluctuation is remarkably reduced, and the resource utilization efficiency is improved.

The invention relates to a spleen-tonifying, appetite-stimulating and digestion-promoting traditional Chinese medicine preparation UPLC detection method and a fingerprint spectrum, and belongs to the technical field of medicine detection. The invention aims to solve the technical problems that the existing quality control method of the spleen-tonifying, appetite-promoting and digestion-promoting pills lacks comprehensive and objective analysis on the spleen-tonifying, appetite-promoting and digestion-promoting pills, and the problems of low precision, poor reproducibility, low efficiency and the like during detection of related components in the prior art are solved. According to the technical scheme, the UPLC detection method is characterized in that a mixed standard substance of gallic acid, 5-hydroxymethylfurfural, chlorogenic acid, sinapine thiocyanate, sinapic acid, hyperoside, 3, 6-dimustard acyl sucrose, hesperidin, naringenin, nobiletin and hesperetin is taken as a contrast, an acetonitrile-0. 1% formic acid aqueous solution is taken as a mobile phase, gradient elution is carried out, and the content of chlorogenic acid in the sample is detected. The qualitative and quantitative detection of the effective components of the spleen-tonifying, appetite-promoting and digestion-promoting pill is realized.

The invention discloses application of a pharmaceutical composition in preparation of a medicine for treating nephropathy, the nephropathy is at least one of membranous glomerulonephritis, postoperative acute kidney injury caused by non-contrast agents, immunoglobulin A nephropathy, purpura nephritis and idiopathic focal segmental glomerulosclerosis, and the pharmaceutical composition belongs to the technical field of pharmacy. The pharmaceutical composition is prepared from the following flavonoid components in a mass ratio: the mass ratio of the gossypothelin-8-O-beta-D-glucuronide to the hyperoside to the isoquercitrin to the myricetin to the quercetin-3 '-O-glucoside is 10 to (3.0 to 20) to (2.0 to 18) to (0.2 to 6.0) to (1 to 20). The pharmaceutical composition has obvious effects of preventing and / or treating membranous glomerulonephritis, postoperative acute kidney injury caused by non-contrast agents, immunoglobulin A nephropathy, purpura nephritis and idiopathic focal segmental glomerulosclerosis, and has relatively good safety.

Disclosed in the present invention are a hyperoside mixed micelle, a preparation method therefor, and use thereof. The hyperoside polymer mixed micelle comprises: hyperoside, TPGS, and F68. The polymers TPGS and F68 are used as carriers for the mixed micelle to encapsulate the hyperoside therein. The hyperoside mixed micelle of the present invention can effectively increase the solubility of the hyperoside, thereby improving its oral bioavailability, reducing adverse drug reactions, and enhancing the therapeutic efficacy against ulcerative colitis.

The invention relates to the field of traditional Chinese medicine quality detection, in particular to a method for detecting the content of chemical components in cotton flower medicinal materials. The detection method comprises the following steps: providing a reference substance solution, wherein a reference substance comprises one or more of hyperoside, isoquercitrin, astragalus smicus glycoside, quercetin, quercetin-7-O-glucoside and quercetin-3 '-glucoside; preparing a test solution by using a cotton flower medicinal material sample to be detected; and detecting the reference substance solution and the test solution by high performance liquid chromatography, and determining the content of chemical components corresponding to the reference substance in the to-be-detected cotton flower medicinal material sample, the high performance liquid chromatography meets one or more of the following conditions: a stationary phase is a C18 chromatographic column; the mobile phase comprises a mobile phase A, a mobile phase B and a mobile phase C, the mobile phase A comprises methanol, the mobile phase B comprises acetonitrile, and the mobile phase C comprises a phosphoric acid aqueous solution. The whole method is high in sensitivity, simple and convenient to operate, relatively low in cost and short in detection time.

The invention discloses an intestinal tract hemostatic composition as well as a preparation method and application thereof, and relates to the field of hemostatic drugs. Wherein the catechin, the hyperoside, the isoquercitrin, the quercetin-3-O-glucuronide, the gallocatechin, the myricetin-3-O-galactoside, the proanthocyanidin B1, the proanthocyanidin B3, the syringe-3-O-glucoside and the astragalus smicus glycoside are flavonoid compounds; nuciferine, higenamine, N-methyl-higenamine and N-trans-feruloyl tyramine are taken as alkaloid compounds, and the compound is selected from the group consisting of higenamine, nuciferine, higenamine The composition has the advantages of simple preparation process, good hemostatic effect and the like, and provides a new raw material choice for the development of intestinal bleeding treatment medicines.

The invention relates to application of combination of hyperoside and salvianolic acid B to preparation of a medicine for treating NAFLD, and belongs to the technical field of biological medicine. The hyperoside and the salvianolic acid B have a synergistic effect in preparation of the medicine for treating the non-alcoholic fatty liver disease when used in combination according to the required treatment amount, the curative effect can be enhanced and the side effect can be reduced through combined use of the two medicines, the effect is better than that of independent medication, a new thought is provided for treating the non-alcoholic fatty liver disease, and the application prospect is wide. The method has important significance and wide application prospects.

The present invention relates to the technical field of oral repair materials, and in particular to an antibacterial oral repair composite membrane and a preparation method thereof. The oral repair composite membrane is composed of an upper antibacterial membrane, a middle polycaprolactone fiber membrane, and a lower bone repair membrane; the raw materials for preparing the upper antibacterial membrane include the following components in parts by weight: 3-4 parts of cinnamon essential oil, 0.5-1 parts of xanthan gum, 0.5-1 parts of seed meal protein, 0.1-0.15 parts of tannic acid, 5-7 parts of silk fibroin, 1-1.5 parts of polylactic acid, 0.6-1 parts of chitosan, 1-1.5 parts of polyvinyl alcohol, and 1.5-2.5 parts of glycerol; the raw materials for preparing the lower bone repair membrane include the following components in parts by weight: 2-3 parts of nanohydroxyapatite, 3-5 parts of citric acid, 6-9 parts of lactic acid, 3-4 parts of tyrosine, 1-2 parts of glycolic acid, 2-3 parts of casein, 0.2-0.3 parts of hyperoside, and 1-2 parts of gelatin.

The invention discloses an acanthopanax endophytic fungus DLCWJ05 for producing hyperoside and acacetin and application of the acanthopanax endophytic fungus DLCWJ05, relates to the field of microorganisms, and aims at solving the problem that the consumption of plant resources is large when hyperoside and acacetin are obtained by an existing method. The endophytic fungi DLCWJ05 of the acanthopanax senticosus are alternaria alternata DLCWJ05 and are preserved in the China Center for Type Culture Collection on July 11, 2025, and the preservation number of the endophytic fungi DLCWJ05 is CCTCC NO: M 20251589. The endophytic fungi DLCWJ05 of the acanthopanax senticosus can be fermented to produce hyperoside and acacetin, and also has antioxidant activity. And a novel method is provided for producing the acanthopanax secondary metabolite by adopting a microbial fermentation method.

To provide a new use of Hibiscus 'Titanbicus'.SOLUTION: An angiotensin-converting enzyme inhibitor contain Hibiscus 'Titanbicus' containing hyperoside and isoquercitrin at a ratio of (1:3) to (2:1), for example, a flower (including bud) or its extract of Hibiscus 'Titanbicus' peach white (kind registration by the Ministry of Agriculture, Forestry and Fisheries No. 21813), and contain a Hibiscus 'Titanbicus' perennial plant, as the flower (including bud) or its extract of Hibiscus 'Titanbicus', wherein a total amount of the hyperoside and the isoquercitrin contained in the Hibiscus 'Titanbicus' perennial plant is 1.0 mass% or more.SELECTED DRAWING: Figure 1

The invention provides a construction method and application of a pepper medicinal material UPLC characteristic spectrum. The construction method of the pepper medicinal material UPLC characteristic spectrum comprises the following steps: taking hyperoside, hydroxyl-alpha-sanshool and hydroxyl-beta-sanshool to prepare a mixed reference substance solution; preparing a test solution from a pepper medicinal material; and respectively carrying out ultra-high performance liquid chromatography analysis on the mixed reference substance solution and the test solution, and constructing the pepper medicinal material UPLC characteristic spectrum. The construction method of the pepper medicinal material UPLC characteristic spectrum has the advantages of being rapid, stable and high in specificity, the obtained pepper medicinal material characteristic spectrum shows 11 characteristic peaks, spectrum information is rich, the chemical component characteristics of the pepper medicinal material can be fully displayed, a comprehensive and effective rapid evaluation method is provided for quality control of the pepper medicinal material, and the method is suitable for popularization and application. The method can realize identification of different primitives and counterfeit products of Chinese prickly ash, and has a wide application prospect.

The invention discloses an application of a brutal dragon liquid active ingredient composition in preparation of a freckle-removing product, the weight ratio of epimedium polysaccharide to rehmannia and hyperoside in brutal dragon liquid active ingredients is 1: (2-4): (3-5), the invention discloses a preparation method of the brutal dragon liquid active ingredient composition, and the brutal dragon liquid active ingredient composition has a good freckle-removing effect.

The present application relates to the application of hyperoside and salvianolic acid B in the preparation of a drug for treating non-alcoholic fatty liver disease, and belongs to the technical field of biological medicine. The hyperoside and salvianolic acid B have a synergistic effect in the preparation of a drug for treating non-alcoholic fatty liver disease when used in combination in a required amount, and the combination of the two drugs can enhance the curative effect and reduce side effects, which is more effective than the use of a single drug, provides a new idea for treating non-alcoholic fatty liver disease, and has important significance and wide application prospect.

The invention discloses a fingerprint detection method for quality control of Huanglong cough and asthma capsules / Huanglong cough granules, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, a high performance liquid chromatography-mass spectrometry technology is adopted, 5-hydroxymethylfurfural, vitexin, hyperoside, irisflorentin, epimedin B, icariin, irisflorentin, sub-irisflorentin and baohuoside I are taken as reference substances, and different batches of test samples are detected by optimizing extraction conditions and chromatographic parameters, so that the fingerprint spectrum is successfully constructed. The method is simple and easy to implement, high in precision and good in reproducibility, multi-component synchronous identification can be achieved at a time, the detection efficiency is greatly improved, and time and cost are saved; the quality of a plurality of characteristic components of the Huanglong cough and asthma capsule / Huanglong cough granules can be accurately, quickly and simultaneously monitored, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect.

The present application relates to the technical field of fruit quality improvement, and particularly relates to application of hyperoside in improving grape fruit hardness. The scheme provided in the present application is verified by a large number of field tests, after spraying the plant with hyperoside solution, the fruit hardness of four grape varieties of'red double taste', 'rose flavor','sunshine rose' and 'italy' is all significantly improved compared with the control group, wherein the hardness of'red double taste' and 'rose flavor' is greatly improved, the hardness is increased by about 20%~25% in the grape ripening period, the hardness of'sunshine rose' and 'italy' is relatively small, the fruit hardness is increased by about 8%~16% in the grape color softening period; in addition to improving the fruit hardness, spraying the hyperoside solution can also promote the color uniformity of the grape fruit of some varieties, increase the soluble solids content, reduce the titratable acid content, and make the fruit flavor better; the method is compatible with the conventional grape planting management measures, is easy to popularize and apply in the grape planting area, and can bring significant economic benefits to the majority of growers.

The invention discloses a quality detection method of smoked plum granules for stopping and benefiting, and belongs to the technical field of quality detection of traditional Chinese medicines. The method comprises the following steps: identifying a characteristic spectrum; the method comprises the following specific steps: (1) respectively preparing a hyperoside reference substance solution, a coumarin reference substance solution, a lobetyolin reference substance solution, a cinnamic acid reference substance solution, a psoralen reference substance solution, an isopsoralen reference substance solution and a limonin reference substance solution; (2) preparing a test solution from the smoked plum granules; and (3) respectively carrying out liquid chromatography detection on the seven reference substance solutions and the test solution. The quality detection method disclosed by the invention is high in accuracy, strong in specificity and reliable in detection result, realizes effective control on the quality of the smoked plum granules, is suitable for modern production requirements, and has important significance on quality detection of traditional Chinese medicines and clinical medication.

The present invention discloses a quality detection method for Wumeizhili granules, belonging to the technical field of quality detection of traditional Chinese medicines. The method includes characteristic spectrum identification, and specifically comprises the following steps: (1) preparing a reference solution of hyperoside, a reference solution of coumarin, a reference solution of codonopsis pilosula, a reference solution of cinnamic acid, a reference solution of psoralen, a reference solution of isopsoralen, and a reference solution of limonin; (2) preparing a test solution using Wumeizhili granules; and (3) performing liquid chromatography detection on the seven reference solutions and the test solution. The quality detection method of the present invention has high accuracy, strong specificity, and reliable detection results, achieving effective quality control of Wumeizhili granules, meeting the needs of modern production, and having important significance for quality detection and clinical use of traditional Chinese medicines.

The invention discloses application of hyperoside to treatment of endometriosis, and aims to evaluate whether hyperoside has an anti-fibrosis effect when applied to a rat endometriosis model by taking fibrosis as a treatment target. The method comprises the following steps: constructing a rat endometriosis model through an autotransplantation method, dividing molded rats into a low-dose group, a medium-dose group, a high-dose group, a model group and a western medicine control group 4 weeks after successful modeling, and laparotomizing and taking out ectopic lesions after respectively taking medicines for 4 weeks. HE staining and masson staining methods are used for mutually proving the fibrosis degree, the expression conditions of fibrosis indexes a-SMA, Co-1, CTGF and Fn are detected in an immunohistochemical mode, and the anti-fibrosis effect of hyperoside is evaluated. As a result, hyperoside can prevent the development of fibrosis in endometriosis, and thus hyperoside can be used as a drug for treating or preventing fibrosis in endometriosis.

The present invention discloses the application of the effective ingredients of Manlong Liquid in the preparation of medicines for improving human immunity. The effective ingredients of Manlong Liquid are hyperoside and psoralen. The mass ratio of the hyperoside to psoralen is 1:(2-4), preferably 1:3. The effective ingredients of Manlong Liquid are added with excipients to first prepare a solid dispersion of the effective ingredients of Manlong Liquid, and then a filler, a disintegrant and a lubricant are added to prepare a sustained-release tablet. It is found that hyperoside and psoralen have a synergistic effect in improving human immunity and have great application prospects.

This invention provides a green preparation method for hyperoside derived from lotus leaves and its application, belonging to the field of natural product separation and purification technology. Addressing the shortcomings of existing preparation methods, this invention, for the first time, uses abundant and inexpensive lotus leaves and their extracts as raw materials, employing green solvents to establish a green, efficient, high-purity, simple, rapid, and scalable method for preparing hyperoside. This provides raw materials for the development of hyperoside series products and therefore has significant practical application value.

The application discloses a quality detection method of a Yishen Qingli Huoxue recipe, which comprises preparation of a test sample solution, preparation of a standard solution, preparation of a standard curve and content determination. The application screens an optimal test sample preparation method, a preparation method of a control sample and optimal chromatographic conditions and mass spectrometric analysis conditions through a large number of experiments, and establishes a content determination method of the Yishen Qingli Huoxue recipe, which can simultaneously determine the contents of 11 compounds with different structural types, such as resveratrol, rhein, formononetin, aloe-emodin, emodin, chlorogenic acid, polydatin, astilbin, hyperoside, calycosin-7-glucoside and beta-ecdysterone. The method is favorable for comprehensively monitoring the quality of the Yishen Qingli Huoxue recipe and guaranteeing clinical curative effects. Moreover, the method has the advantages of good stability, high accuracy, good repeatability and high precision.

The application discloses a use of hyperoside in resisting equid herpesvirus 8 (EHV-8) infection. Through qPCR, Western Blot and other experimental techniques, it is revealed on multiple levels that the hyperoside can significantly inhibit the replication of EHV-8, and the mechanism of the hyperoside in resisting the EHV-8 infection is clarified from the aspect of blocking the internalization of the EHV-8, so that the hyperoside can be applied in preparing an anti-EHV-8 medicine.

To provide a new use of Hibiscus 'Titanbicus'.SOLUTION: An anti-anxiety agent and an angiotensin-converting enzyme inhibitor contain Hibiscus 'Titanbicus' containing hyperoside and isoquercitrin at a ratio of (1:3) to (2:1), for example, a flower (including bud) or its extract of Hibiscus 'Titanbicus' peach white (kind registration by the Ministry of Agriculture, Forestry and Fisheries No. 21813), and contain a Hibiscus 'Titanbicus' perennial plant, as the flower (including bud) or its extract of Hibiscus 'Titanbicus', wherein a total amount of the hyperoside and the isoquercitrin contained in the Hibiscus 'Titanbicus' perennial plant is 1.0 mass% or more.SELECTED DRAWING: Figure 1

The invention discloses an establishment method and application of a fingerprint spectrum of a Sanyone-fish-dragon mixture. The establishment method mainly comprises the steps of preparation of a test solution, preparation of a mixed reference substance solution, high performance liquid chromatography detection, establishment of a standard fingerprint spectrum and the like. According to the invention, the standard fingerprint spectrum of the Sapocorrhiza mixture is established, and the method is high in sensitivity, strong in specificity, easy to operate and accurate in result. The standard fingerprint spectrum constructed by the method has 36 characteristic peaks, 8 chemical components such as hesperidin, naringin, ammonium glycyrrhizinate, liquiritin, amygdalin, ferulic acid, hyperoside, quercetin and the like are effectively identified, the problem that the evaluation index of the Sanyone mixture is single is solved, the overall quality condition can be efficiently and comprehensively reflected, and the method has good application prospects. And a basis is provided for quality control and identification of the Sapococalyx mixture.

The invention belongs to the field of gene engineering, and relates to a gene combination for synthesizing homoeriodictyol, a recombinant plasmid combination, a strain and a method for heterologous biosynthesis of homoeriodictyol. The gene combination comprises the following genes: a flavonoid 3 '5'-hydroxylase gene EfF3 '5' H-Unigene 37679, a flavonoid 3 '5'-hydroxylase gene EfF3 '5' H-Unigene 37679, a flavonoid 3 '5' The flavonoid 3 '-hydroxylase genes are EfF3' H-CL3877-2 and EfF3 'H-CL13142-2, and the flavonoid 3'-hydroxylase genes are EfF3 'H-CL3877-2 and EfF3' The flavonoid 3 '-hydroxylase gene EfFOMT-Unigene 24812 has the structural formula shown in the specification. The invention provides a potential method for replacing traditional artificial cultivation or submerged fermentation acquisition, and lays a foundation for further transforming efficient biosynthesis through metabolic engineering.

The invention discloses a medicine detection method, which can simultaneously determine the contents of beta-asarone, schizandrin, hyperoside, verbenin, morinda officinalis verbenin, glycyrrhizic acid and other components in a medicine, and can be used for quality control of the medicine.

The invention discloses glycosyl transferase IlUGT88A3 and application of the glycosyl transferase IlUGT88A3 in flavonoid glycoside synthesis, and belongs to the technical field of gene engineering and enzyme engineering. The glycosyl transferase IlUGT88A3 can be used for specifically catalyzing hydroxyl at C3 site of quercetin to generate glucosylation and galactosylation, so that the isoquercitrin and the hyperoside are efficiently synthesized. The optimum pH is 7.0-7.5, the optimum temperature is 30 DEG C, and the reaction conditions are mild; the method has good substrate heterogeneity, and can be used for carrying out site-specific glycosylation modification on various flavonoid receptors by utilizing sugar donors such as UDP-glucose, UDP-galactose, UDP-rhamnose, UDP-xylose and the like. According to the method, the blank of shortage of flavonoid 3-O-galactosyl transferase resources is filled, the catalytic specificity is high, the efficiency is high, the product is pure, the method can be used for in-vitro enzymatic synthesis and plant heterologous synthesis of high-value flavonoid glycosides such as hyperoside, and a core enzyme element and a technical support are provided for green and efficient preparation of medical raw materials.

The application discloses application of 2''-O-galloylgeniposide in preparation of anti-fibrosis drugs. In the application, the 2''-O-galloylgeniposide can inhibit up-regulation of cell alpha-smooth muscle actin (alpha-SMA) and / or fibronectin expression and up-regulation of type I collagen (CO11A1) gene, fibronectin (FN1) gene and / or alpha-smooth muscle actin (ACTA2) gene transcription in a lung epithelial cell interstitial transformation model, a skin fibroblast activation model, a liver stellate cell activation model and a bleomycin-induced mouse lung fibrosis animal model, and the 2''-O-galloylgeniposide shows a significant anti-fibrosis effect and can be used for preparation of anti-fibrosis drugs.