339 results about "Ischemia" patented technology

The invention is a delivery catheter, e.g., a guide catheter, having an imaging element in proximity to the distal end of the catheter. Catheters of the invention are able to placement of the catheter in proximity to an ostium, thereby increasing the efficiency of contrast delivery while reducing the risk of ischemia due to blocked blood supply. The invention additionally assists in maintaining catheter position during delivery of a fluid from the catheter, resulting in better performance with less fluid. For example, a catheter of the invention can be used to produce improved fluoroscopic images with less overall contrast. This improvement reduces the risk of an allergic reaction to the contrast while decreasing the length of time for a procedure, i.e., because of a need to re-contrast.

The invention relates to a system and method for preparing a surgical suture with controllable knotting force based on a slipknot, and the method comprises the steps: weaving a slipknot controller with a specific topological structure at the tail end of the surgical suture, inputting the required knotting force into a preset force calculation system based on a finite element algorithm, and carrying out the calculation to obtain a preset force; the force control stretching device loads the preset force to the prefabricated force bearing end of the slipknot controller until the slipknot controller reaches the preset load, and preparation of the operation suture with the controllable knotting force is completed. The knotting force can be accurately controlled in the actual suture knotting operation, and tissue cutting and ischemia caused by too large knotting force and tissue leakage caused by too small knotting force are avoided. In the suturing process, clear mechanical signals can be provided for surgeons, the consistency and accuracy of knotting operation are improved, and low-age doctors can complete high-quality knotting.

The invention provides an intravascular occluder which comprises an occluder body, a catheter part and a control mechanism, the occluder body is of an umbrella-shaped structure formed by a plurality of frameworks which can be opened, and a layer of covering is attached to the occluder body; the catheter part is used for accommodating the plugging device and the connecting rod part of the control mechanism; the control mechanism comprises a connecting rod part and a handle control part, and is used for controlling the pushing and withdrawing of the plugging device; after the plugging device is unfolded to completely plug the inner diameter of the blood vessel, the function of cutting off blood flowing can be achieved, a space for blood flowing can be reserved at the position close to the inner wall of the blood vessel by properly retracting the plugging device into the catheter, and the plugging device can be completely retracted into the catheter by completely retracting the plugging device. The device has the advantages that unfolding and folding are completely reversible, controllable opening and cutting of blood flow can be achieved, complications caused by long-time ischemia of blocked blood vessels can be avoided, and a long-time operation becomes possible.

The invention discloses a construction method of a cerebral artery ischemia reperfusion model, and belongs to the technical field of animal models. Three-dimensional positioning coordinates of the middle cerebral artery are obtained through magnetic resonance scanning, the far end of the middle cerebral artery can be accurately positioned, the middle cerebral artery is induced to be blocked after irradiation by setting the blood vessel irradiation position, the rose red dosage and the laser irradiation intensity and time, blood vessel recanalization is caused along with blood impact within 4-5 hours, and finally an ischemia reperfusion model is formed. Compared with a traditional craniotomy electrocoagulation middle cerebral artery (permanent occlusion) and a traditional balloon embolism middle cerebral artery (ischemia reperfusion), the construction method has the advantages that wounds on animals are smaller, the animals are easy to survive, and compared with traditional photochemical induction which can only form infarction lesions on the surface layer of the brain, the method can induce deep brain tissue to form infarction, so that the method has the advantages of being simple and convenient to operate and the like. The method can also be expanded to other brain areas to induce deep vascular occlusion. Meanwhile, the method is also suitable for carrying out model construction on old animals, and the modeling success rate is increased.

The invention relates to the technical field of medical instruments, in particular to a tissue tray assembly used in an abdominal surgery, which comprises a tray and a traction device, and is characterized in that the tray comprises a tray body and a plurality of support pieces, and the plurality of support pieces are of a scattered structure distributed on the tray body; the supporting pieces are divided into first supporting pieces and second supporting pieces, and the first supporting pieces and the second supporting pieces are distributed in a staggered mode. The supporting piece comprises a sleeve body and a telescopic rod, the telescopic rod is connected with an air pipe, and the amount of air in the telescopic rod is changed by injecting air into or exhausting air from the air pipe, so that the telescopic rod is extended or shortened; the telescopic rod corresponding to the first supporting piece and the telescopic rod corresponding to the second supporting piece are independent in motion form. According to the tissue tray assembly used in the abdominal cavity operation, the situation that tissue in the abdominal cavity is pressed for a long time can be avoided, the situation that blood vessels in the tissue are pressed for a long time is avoided, and the situation that the supported part on the tissue is insufficient in blood supply or ischemia or hypoxia occurs is avoided.

The invention discloses a man-machine suitability evaluation method for a mattress, and relates to the field of human engineering health monitoring, and the method comprises the steps: screening testers according to a BMI grading standard, and collecting the height, weight, gender, age, sleep habit and chronic pain medical history basic data of the testers to obtain the BMI values of the testers; inputting the BMI value of the tester into the mattress hardness selection model to obtain the hardness range of the tested mattress, arranging a to-be-tested mattress with selected hardness in a constant-temperature and constant-humidity experimental environment, and enabling the tester to lie on the to-be-tested mattress in sequence at a standard supine position and a standard lateral position. According to the method, the spine stiffness demand curve is predicted in real time through the LSTM neural network, millisecond-level dynamic support adjustment is achieved, the response speed is remarkably increased compared with a traditional method, and spine compensatory bending in sleep can be prevented; and in combination with pressure-blood oxygen coupled IRI index calculation, microcirculation disturbance is accurately warned before tissue injury, and the problem of local ischemia is solved through partition adjustment.

The invention discloses a local oxygen therapy, flushing and skin stretching device for chronic wound treatment, and relates to the technical field of medical apparatuses and instruments, the local oxygen therapy, flushing and skin stretching device comprises a stretching disc, and a cleaning device is arranged in the middle of the stretching disc; according to the device, breakthrough is achieved through the combination of the circular structure of the stretching disc and the circumferentially-distributed T-shaped sliding racks; the circular geometric structure of the stretching disc has stress symmetry, and stretching force can be evenly dispersed; the position of the T-shaped sliding rack installed in a circumferential sliding mode can be flexibly adjusted in the radial direction of the stretching disc, and six or more stretching points can be set at will according to the boundary of the wound surface in cooperation with the sliding block and the T-shaped hook frame which can slide. For example, for an irregular polygonal wound surface of a diabetic foot, a medical worker can mark six stress balance points on the T-shaped sliding rack and fix the hook, synchronous or independent adjustment of multiple distraction points is achieved through linkage of the rotating gear ring and the multiple sets of gears, and the situation that skin ischemia blister is caused by too large local stress or tissue stretching is affected by insufficient stress is avoided.

The present invention pertains to a polypeptide comprising the amino acid sequence according to SEQ ID NO: 1 or a variant or fragment thereof for use in treating or preventing heart failure, myocardial infarction or hypertrophy, reducing infarct size or scar size after myocardial infarction, inducing endothelial cell sprouting after myocardial infarction, protecting cells from ischemia- or reperfusion-induced injury, promoting angiogenesis after myocardial infarction, promoting coronary endothelial cell migration and proliferation, inhibiting cardiomyocyte hypertrophy, or inhibiting inflammation. The present invention further provides a nucleic acid sequence encoding the polypeptide, vectors comprising the nucleic acid sequence, host cells comprising the nucleic acid or vector, and respective pharmaceutical compositions for the same use.

The invention provides blood transfusion equipment and a blood transfusion method, the blood transfusion equipment is used for transfusing blood to a donor, and the blood transfusion equipment comprises a main pipeline used for conveying blood; the first end of the first branch pipeline is communicated with the main pipeline, and the second end of the first branch pipeline is used for supplying blood to a donor; the first end of the second branch pipeline is communicated with the main pipeline; the bubble sensor is arranged on the main pipeline, and the bubble sensor is used for detecting bubbles in blood in the main pipeline; the valve device is switched between a first state and a second state, when the valve device is in the first state, blood in the main pipeline flows to the first branch pipeline, and when the valve device is in the second state, the blood in the main pipeline flows to the second branch pipeline. According to the blood transfusion equipment, bubbles in blood flowing to a donor can be eliminated more quickly, and the ischemia time of the donor is shortened.

The invention relates to the technical field of intraoperative intelligent monitoring, and particularly discloses a spinal canal debonding method and system for real-time state evaluation, and the method comprises the steps: collecting neural structure microcirculation blood flow data in real time through integrating a micro-sensing unit at a working end part of a surgical instrument, and extracting blood flow oscillation frequency and capillary hematocrit parameters; performing multi-scale signal decomposition on the blood flow oscillation frequency to obtain an energy distribution parameter, and performing dynamic mode recognition on the hematocrit data to obtain a main variation characteristic value; fusing the two characteristic parameters to generate a nervous tissue vitality index; inputting the index into a pre-trained neural network for time sequence feature analysis, and outputting a graded ischemia risk early warning signal; the operation rhythm is automatically adjusted through a decision tree control mechanism based on the early warning signal, and self-adaptive regulation and control according to the state of the nervous tissue are achieved; the technical problem that the risk of neural ischemia cannot be monitored in real time in a traditional spinal canal release operation is solved, and occult nerve injury caused by asymptomatic neural ischemia can be prevented.

The invention provides a lipoic acid-based nano-enzyme and a preparation method and application thereof, firstly, lipoic acid is dissolved in an organic solvent by utilizing the amphipathy of the lipoic acid and is self-assembled in water to form a nano-aggregate, and then ring opening polymerization of the lipoic acid is initiated by ultraviolet irradiation to obtain stable lipoic acid nano-particles, namely the lipoic acid-based nano-enzyme. The lipoic acid-based nano-enzyme has the beneficial effects that the preparation method of the lipoic acid-based nano-enzyme prepared by the method is simple, the lipoic acid-based nano-enzyme has stable particle size, good active oxygen scavenging ability and blood brain barrier crossing ability, and in addition, a cavity in the nano-enzyme is suitable for loading hydrophobic bioactive factors and drugs, so that the lipoic acid-based nano-enzyme has a good application prospect. Rich carboxyl groups on the surface of the nano-enzyme can be complexed with various ions, and the nano-enzyme can be applied to treatment of inflammation diseases caused by hypoxia or ischemia and the like and brain drug delivery.

The present invention relates to amide derivatives of Formula (XIIIa) and pharmaceutical compositions thereof that modulate the activity of the PGI2 receptor.Compounds of the present invention and pharmaceutical compositions thereof are directed to methods useful in the treatment of: pulmonary arterial hypertension (PAH); idiopathic PAH; familial PAH; PAH associated with a collagen vascular disease, a congenital heart disease, portal hypertension, HIV infection, ingestion of a drug or toxin, hereditary hemorrhagic telangiectasia, splenectomy, pulmonary veno-occlusive disease (PVOD) or pulmonary capillary hemangiomatosis (PCH); PAH with significant venous or capillary involvement; platelet aggregation; coronary artery disease; myocardial infarction; transient ischemic attack; angina; stroke; ischemia-reperfusion injury; restenosis; atrial fibrillation; blood clot formation in an angioplasty or coronary bypass surgery individual or in an individual suffering from atrial fibrillation; atherosclerosis; atherothrombosis; asthma or a symptom thereof; a diabetic-related disorder such as diabetic peripheral neuropathy, diabetic nephropathy or diabetic retinopathy; glaucoma or other disease of the eye with abnormal intraocular pressure; hypertension; inflammation; psoriasis; psoriatic arthritis; rheumatoid arthritis; Crohn's disease; transplant rejection; multiple sclerosis; systemic lupus erythematosus (SLE); ulcerative colitis; ischemia-reperfusion injury; restenosis; atherosclerosis; acne; type 1 diabetes; type 2 diabetes; sepsis, and chronic obstructive pulmonary disorder (COPD).

A method and a system of generating a color-coded image demonstrating blood flow in a chosen area in a patient, specifically for diagnosing medical conditions like ischemia, strokes and infarcts. A plurality of images, preferably 3, are taken at different points in time that are a few seconds apart, and colorized by different colors, preferably a first image red, a second image blue and a third image green. These colorized images are then aligned and summated to create a multicolor image that time encodes perfusion of blood. Preferably, said method is performed after injection of a contrast agent.

The invention discloses a construction method and application of an iPSC-derived mesenchymal stem cell strain for overexpressing a VEGFA gene based on a CRISPR / Cas9 technology, and relates to the technical field of biological cells, the construction method of the iPSC-derived mesenchymal stem cell strain for overexpressing the VEGFA gene based on the CRISPR / Cas9 technology comprises the following steps: S1, designing sgRNA and ssDNA for an insertion site AAVS1, and detecting the cutting efficiency of the sgRNA; s2, introducing a CRISPR (clustered regularly interspaced short palindromic repeats) system into the cells in an electroporation manner to obtain an iPSC cell strain into which an exogenous gene VEGFA is inserted at a fixed point; s3, carrying out directional induced differentiation on the iPSC cell strain to the iMSC to obtain an iMSC cell strain with overexpression of VEGFA (Vascular Endothelial Growth Factor A) protein; the iMSC of the overexpressed VEGFA protein can well stimulate vascular endothelial growth and promote angiogenesis, and more possibilities are provided for repair treatment of ischemia-related diseases.

The utility model relates to a circumcision anastomat which comprises a glans penis cover. The glans penis cover comprises a cover body, and an opening is formed in the middle of the front end of the cover body. An inwards-concave notch is formed in the front end of the cover body in the circumferential direction, an inner ring is assembled on the inwards-concave notch, an outer ring matched with the inner ring is assembled on the outer side of the inner ring, the outer ring penetrates through the cover body and is connected with the inner ring, multi-pole magnetic fields are arranged in ring bodies of the inner ring and the outer ring, and the inner ring and the outer ring can be axially attached to each other, attracted to each other and clamped with each other; the prepuce is clamped between the inner ring and the outer ring, meanwhile, the inner diameter of the inner ring is smaller than that of the outer ring, the prepuce is clamped through the difference between the inner diameters of the inner ring and the outer ring, a mechanical compression effect is generated on tissue through mutual attraction force between the magnets, and therefore the squeezed tissue is subjected to dry necrosis due to ischemia; adjacent tissues on the outer edge of the squeezing area are layered and healed to form an anastomotic stoma, and local swelling is light. And postoperative scar formation is reduced.

The invention relates to the technical field of stem cells, and particularly discloses application of mesenchymal stem cells (MSC) in treatment of lower limb vein ischemia. According to the application, the SDF1-FGF2 fusion protein is used for pretreating human mesenchymal stem cells (hMSCs), and the cell activity, the blood flow recovery capacity and the anti-inflammatory effect of the hMSCs in a lower limb vein ischemia model can be remarkably improved through pretreatment. The hMSCs pretreated by SDF1-FGF2 are injected into the body of a venous ischemia model mouse, and compared with untreated cells, the hMSCs have better effects in the aspects of improving blood perfusion, reducing inflammatory response, recovering motor function and the like. The method provides an efficient stem cell treatment strategy for vascular diseases such as lower limb vein ischemia.

A system and method of measurement and calculation of intracardiac pressures based on non-invasive medical imaging is presented, wherein the pressure measurements are performed by means of the image stream with further estimation of the volumes of oscillating traceable regions within the heart vicinity. The volume estimates are tied to pressure values in such oscillating traceable regions as left / right atria and ventricles, pulmonary artery and aorta. The invention permits to assess non-invasively and in real time the pressure in any part of the heart and large blood vessels, and calculate the major markers of heart failure, cardiomyopathy, ventricular ischemia, infarction and other heart related diseases.

The invention discloses an application and method of SAH-CIM hydrogel in promoting survival of retinal ganglion cells in glaucoma. The hydrogel comprises a Y-type primitive composed of three DNA chains and a siRNA connector, and the nucleotide sequences of the three DNA chains are SEQ ID NO.1-SEQ ID NO.3; the method comprises the following steps: S1, constructing a Y-type primitive: mixing three DNA chains with equal molar concentration in an annealing buffer solution, performing high-temperature denaturation at 95 DEG C, and then quickly cooling to 4 DEG C to finish annealing, so as to form a stable Y-type structure with three-way cohesive ends; s2, synthesizing a siRNA (small interfering Ribonucleic Acid) connector; and S3, mixing the Y-type element and the siRNA connector according to a molar ratio of 1: 1.5 at room temperature to react for 30 minutes, and forming a three-dimensional network structure through complementary pairing driven self-assembly, thereby finally obtaining the SAH-CIM hydrogel. The SAH-CIM hydrogel provided by the invention can be used for preventing / treating ischemia-reperfusion injury and related diseases, for example, the SAH-CIM hydrogel can be used for treating glaucoma, accurate co-delivery of a neuroprotective agent and an immunomodulator can be realized, and the SAH-CIM hydrogel has the advantages of treatment accuracy and biocompatibility.

The invention discloses an electrocardio data processing and coronary heart disease risk prediction method, device and system and a medium. The method comprises the steps of obtaining exercise electrocardio data output through at least one electrocardiogram lead; analyzing the exercise electrocardiogram data to obtain a heart rate sequence corresponding to each electrocardiogram lead; extracting a QRS wave group corresponding to each heart beat from the exercise electrocardio data; dividing a plurality of heart rate intervals according to the obtained heart rate sequence; according to the heart rate interval to which the heart rate corresponding to each QRS wave group belongs, classifying the QRS wave group into a group corresponding to the corresponding heart rate interval; determining an average QRS wave group corresponding to each heart rate interval according to all the QRS wave groups in each group; extracting the high-frequency energy of the average QRS wave group corresponding to each heart rate interval; and determining an energy sequence according to the high-frequency energy corresponding to each heart rate interval. According to the scheme, high-frequency components and dynamic load information which are more sensitive to ischemia are utilized, so that the detection rate and the identification capability of coronary heart diseases, particularly early and mild lesions, can be remarkably improved.

The invention discloses a perfusion balloon and a balloon dilatation catheter, and relates to the technical field of medical instruments. The perfusion balloon comprises a fillable body and at least one hollow component, a concave part and a convex part which are connected with each other at least in a full state are formed on the peripheral wall of the fillable body; the at least one hollow assembly is arranged on the peripheral wall of the fillable body and extends in the length direction of the fillable body, and the hollow assembly and the sunken part jointly form a blood flow channel allowing blood to pass through. The structure of the perfusion balloon is improved, a blood flow channel is formed between the periphery of the perfusion balloon and the blood vessel wall, fluid in the blood vessel is allowed to flow when the balloon expands, longer balloon expansion time is allowed, and the risks of ischemia and tissue damage are reduced.

The invention discloses a bionic bacterial outer membrane nano-particle for drug delivery. The bionic bacterial outer membrane nano-particle is characterized by comprising low-toxicity outer membrane vesicles extracted from escherichia coli with msbB genes knocked out, and low-toxicity outer membrane vesicles extracted from escherichia coli with msbB genes knocked out; the medicine carrying liposome is prepared from phosphatidylcholine, cholesterol and DSPE-PEG2000 (Distearoyl Phosphatidylcholine-Polyethylene Glycol 2000) according to a preset mass ratio; the low-toxicity outer membrane vesicles and the drug-loaded liposome are subjected to ultrasonic assistance and 100nm filter membrane extrusion to obtain bionic bacterial outer membrane nanoparticles, the average particle size of the bionic bacterial outer membrane nanoparticles is about 100nm, and the encapsulation efficiency exceeds 85%. The BM-NPs system disclosed by the invention can load small molecule compounds, proteins, nucleic acids and gene therapy vectors, and is suitable for various central nervous system diseases such as spinal cord injury, cerebral apoplexy, traumatic brain injury, ischemia-reperfusion injury and the like.

A system for monitoring medical conditions including pressure ulcers, pressure-induced ischemia and related medical conditions comprises at least one sensor adapted to detect one or more patient characteristic including at least position, orientation, temperature, acceleration, moisture, resistance, stress, heart rate, respiration rate, and blood oxygenation, a host for processing the data received from the sensors together with historical patient data to develop an assessment of patient condition and suggested course of treatment, including either suspending or adjusting turn schedule based on various types of patient movement. The sensor can include bi-axial or tri-axial accelerometers, as well as resistive, inductive, capacitive, magnetic and other sensing devices, depending on whether the sensor is located on the patient or the support surface, and for what purpose.

The flow-adjustable perfusion balloon catheter comprises a catheter body, a balloon capable of being filled and a catheter base, a push rod channel and a filling channel are arranged in the catheter body, the filling channel is communicated with an inner cavity of the balloon, and a first connector and a second connector are arranged on the catheter base. Micropores are formed in the positions, located at the near end and the far end of the balloon, of the catheter body and are the near-end micropore and the far-end micropore respectively, the near-end micropore and the far-end micropore are communicated through a blood flow channel formed in the catheter body, and a blocking piece capable of blocking the near-end micropore or the far-end micropore is arranged in the blood flow channel. A push rod is arranged in the push rod channel, and the far end of the push rod is connected with the near end of the plugging piece. Compared with the prior art, by arranging the blood flow channel, effective circulation of blood flow can still be achieved after balloon plugging in the operation, complications caused by ischemia or hypertension in the operation are avoided, the perfusion blood flow can be adjusted in time according to different patient conditions, and the success rate of the operation is increased.

The invention discloses a pancreas strapping tape capable of quantifying strapping pressure, and belongs to the technical field of medical instruments, the pancreas strapping tape comprises a first non-extension section and a second non-extension section, an elastic section and a limiting rope are arranged between the first non-extension section and the second non-extension section, and an insertion hole is formed in the head end of the first non-extension section. The quantitative binding force is provided for the pancreatic stump, sufficient bursting pressure can be provided for the main pancreatic duct, complete pancreatic ischemia and acute pancreatic necrosis caused by too high binding pressure are avoided, and the risk of pancreatic fistula and pancreatitis caused by acute pancreatic necrosis is reduced; due to the design of the limiting rope, whether preset binding tension is reached or not can be easily judged in the binding process; when pancreatic tissue is shrunk in the postoperative process, the strapping tape can still continue to provide strapping pressure through contraction of the elastic section, the strapping pressure generated in the postoperative process is reduced in a limited mode, enough bursting pressure can still be provided for the main pancreatic duct, and the risk of pancreatic fistula caused by insufficient bursting pressure of the main pancreatic duct is reduced.

The invention belongs to the field of medicinal chemistry, and particularly relates to a cyclovirobuxine D derivative compound shown as a structural formula (I) or a structural formula (Ia), a preparation method and application, experimental results show that the compound shown as the structural formula (I) or the structural formula (Ia) has different degrees of hypoxia and ischemia resistance, so that the compound can be used as a medicine for preventing or treating cardiovascular and cerebrovascular diseases and can be used for preparing medicines for preventing or treating cardiovascular and cerebrovascular diseases. The polypeptide has the potential of treating cardiovascular and cerebrovascular diseases. The cardiovascular and cerebrovascular diseases include but are not limited to angina pectoris, arrhythmia, hypertension, hyperlipidemia and cerebral arterial thrombosis. # imgabs0 #

The present invention relates to an injectable pre-formulation comprising:a) at least one of a mono-, di- or tri-acyl lipid and / or a tocopherol;b) optionally at least one phospholipid;c) at least one biocompatible, organic solvent; andd) at least one prostacyclin analogue or a salt thereof;wherein the pre-formulation forms, or is capable of forming, at least one liquid crystalline phase structure upon contact with excess aqueous fluid. Such compositions may additionally comprise polar co-solvents. Methods of treatment, particularly for management of pulmonary artery hypertension (PAH), severe PAH, Raynaud's disease, ischemia and related conditions are provided, as well as corresponding uses of the compositions. Administration devices comprising the formulations and kits comprising the devices are also provided.

The utility model relates to medical instrument technical field especially is a kind of wearable femoral vein compression hemostasis device, including disposable underpants body, the upper surface of one side of trouser leg is located at femoral vein and is fixedly installed with the compression assembly for fixing wound, and the compression assembly includes clamping ring plate, the middle part of clamping ring plate is equipped with four groups of insertion slot, the surface both sides of waistband are fixedly installed with trouser loop, and the periphery of waistband is provided with the bandage assembly for exerting pressure and is set, and the bandage assembly includes waist bandage and leg bandage, the both ends of waist bandage are insertedly installed in the inside of insertion slot, the both ends of leg bandage are also insertedly installed in the inside of insertion slot, the both ends of waist bandage can be folded and detachably connected, and the both ends of leg bandage can be folded and detachably connected.The utility model is folded and detachably connected by underpants design, bandage, clamping ring plate disperses pressure, reduces the risk of local ischemia, and waist bandage and leg bandage synergistic effect, form three-dimensional compression.

A method, device, system and medium for electrocardio data processing and coronary heart disease risk prediction, motion electrocardio data output through at least one electrocardio lead is acquired; the motion electrocardio data is analyzed to obtain a heart rate sequence corresponding to each electrocardio lead; each QRS complex corresponding to each heartbeat is extracted from the motion electrocardio data; a plurality of heart rate intervals are divided according to the obtained heart rate sequence; each QRS complex is classified into a group corresponding to a heart rate interval according to the heart rate interval to which the heart rate corresponding to the QRS complex belongs; the average QRS complex corresponding to each heart rate interval is determined according to all QRS complexes in each group; the high-frequency energy of the average QRS complex corresponding to each heart rate interval is extracted; and an energy sequence is determined according to the high-frequency energy corresponding to each heart rate interval. The present scheme uses high-frequency components and dynamic load information which are more sensitive to ischemia, and can significantly improve the detection rate and discrimination ability for coronary heart disease, especially early and mild lesions.

The invention discloses a GLP-1 (32-36) engineered exosome and application of the GLP-1 (32-36) engineered exosome in drugs and instruments for preventing and / or treating diabetic lower limb arterial lesion. The preparation process and related characteristics of the GLP-1 (32-36) engineered exosome are respectively as shown in figures (1) and (2). The exosome is prepared by treating human pluripotent stem cells by using GLP-1 (32-36). The GLP-1 (32-36) engineered exosome has relatively high activity in plasma, and the enterotrypsin synthetic peptide can effectively improve the yield of the exosome and further optimize the function of the exosome, so that a stronger therapeutic effect of promoting angiogenesis after ischemia is exerted. The GLP-1 (32-36) engineered exosome disclosed by the invention also has safety and can be used for a treatment strategy of diabetic lower limb artery complications.

The present invention relates to compounds with high chemical stability and methods for inhibiting the pathological activity of extracellular histones in a subject. In particular, the invention relates to compounds with high chemical stability, uses thereof and methods for inhibiting or ameliorating extracellular histone mediated ailments (such as, for example, sepsis, systemic immune response syndrome (SIRS) and ischemia reperfusion injury (IRI)). More particularly, the invention relates to methods and uses of a polyanionic sulfated cellobioside modified with a small uncharged glycosidically linked substituent at its reducing terminus, wherein the presence of the substituent results in a molecule with high chemical stability without affecting the ability of the molecule to be effective in the therapy of extracellular histone mediated ailments. For example, the present invention relates to methods and uses of β-O-methyl cellobioside sulfate (mCBS) or a pharmaceutically acceptable salt thereof (e.g., mCBS.Na), in the therapy of a range of extracellular histone mediated ailments in subjects.