269 results about "Acetylcholine" patented technology

The invention belongs to the technical field of monatomic nano-enzyme material preparation, and particularly relates to a nano-enzyme preparation method for acetylcholin esterase activity detection. The Ni-NiO heterojunction material is successfully synthesized by adopting Ni-MOF as a carrier and accurately regulating and controlling the ratio of nitrogen to air in the ultrafast Joule hot calcination process. The material has rich oxygen vacancies and metal Ni sites, the oxygen vacancies and the metal Ni sites can effectively serve as anchoring sites of Pt single atoms, the loading capacity and dispersity of the Pt single atoms are remarkably improved, and therefore the excellent nano-enzyme catalytic activity is shown. In addition, the preparation method disclosed by the invention is simple and convenient in process and has a wide industrial application prospect. An acetylcholin esterase activity colorimetric sensing method constructed on the basis of the nanometer enzyme for acetylcholin esterase activity detection prepared by the method has extremely high sensitivity and low detection limit. The detection limit of the technology can be as low as 0.037 mU / mL, the problem that the sensitivity of a traditional detection method in a low-concentration sample is insufficient is remarkably solved, and remarkable clinical application potential is shown.

The invention provides an RPA-CRISPR-Cas12a system-based detection reagent for the drug resistance of a chilo suppressalis organophosphorus insecticide, a detection method and application, and belongs to the technical field of molecular detection. The invention provides a detection reagent for chilo suppressalis organophosphorus insecticide resistance, the reagent comprises a specific RPA2 primer pair designed for chilo suppressalis acetylcholin esterase ace-1 gene A314S resistance mutation site and crRNA, a double screening mechanism of RPA amplification and CRISPR-Cas12a is combined, wild type and mutant type genes can be accurately distinguished, and the detection reagent can be used for detecting the insecticide resistance of the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis. The rapid, sensitive and visual nucleic acid detection of the chilo suppressalis ace-1 gene A314S resistance mutation site is realized, and a visual detection means which is high in specificity, simple and convenient to operate, economical and efficient is provided for field on-site determination of chilo suppressalis resistance populations.

The invention belongs to the technical field of biological information, and relates to an acetylcholin esterase inhibitor prediction method based on Stacking ensemble learning and molecular feature fusion, which comprises the steps of data collection and preparation, data annotation and optimization, feature extraction and analysis, construction of a Stacking model, result verification and feedback and construction of a prediction platform. The molecular fingerprints and the property descriptors are used as features, and an acetylcholin esterase inhibitor classifier is successfully constructed by adopting a Stacking algorithm. According to the method, the problems that the efficiency of finding the acetylcholin esterase inhibitor by a traditional experimental method is low, and a common quantitative structure-function relationship method is high in complexity and poor in generalization ability can be solved, the new drug finding speed is increased, experimental candidates are accurately positioned, and resource waste is reduced.

The invention provides therapeutic methods, pharmaceutical compositions, and unit dose formulations for treating movement disorders, such as using a muscarinic acetylcholine receptor inhibitor in combination with a muscarinic acetylcholine receptor activator to treat dystonia. In some aspects, the invention provides a method of treating a movement disorder in a patient, where the method comprises administering to a patient in need thereof (i) a muscarinic acetylcholine receptor inhibitor in an amount effective to treat the movement disorder and (ii) a muscarinic acetylcholine receptor activator in an amount effective to reduce the frequency and / or magnitude of at least one side effect of the muscarinic acetylcholine receptor inhibitor, to thereby treat the movement disorder.

This invention discloses a protein and gene related to resistance to neonicotinoid acetylcholine receptor competitive regulator insecticides in peach aphids, and their applications. It relates to the field of screening technology for neonicotinoid acetylcholine receptor competitive regulator insecticides in peach aphids. This invention obtains and clarifies an MpeABCC_4G protein and its encoding gene that is closely related to the resistance of peach aphids to neonicotinoid acetylcholine receptor competitive regulator insecticides. By knocking down the expression of MpeABCC_4G in resistant peach aphids using RNAi technology, the control efficacy of neonicotinoid acetylcholine receptor competitive regulator insecticides is significantly improved.

This invention belongs to the field of microbial technology, specifically relating to a strain of *Pediococcus pentosaceus* SUN02 with acetylcholinesterase inhibitory activity and its applications. This *Pediococcus pentosaceus* SUN02 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 29705. The *Pediococcus pentosaceus* SUN02 provided by this invention exhibits strong acid and bile salt resistance, good tolerance in simulated gastric and intestinal fluids, and can successfully reach the human intestine. *Pediococcus pentosaceus* SUN02, in its bacterial suspension and inactivated cells, possesses strong acetylcholinesterase inhibitory activity, produces γ-aminobutyric acid (GABA), and has antioxidant and other probiotic functions. Its application in probiotic products may help improve Alzheimer's disease, enhance learning and memory abilities, regulate mood, and improve sleep.

The invention belongs to the technical field of microorganisms, and relates to lactobacillus acidophilus LA4 and application thereof. The classification name of the lactobacillus acidophilus LA4 is lactobacillus acidophilus, the lactobacillus acidophilus LA4 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 9, 2025, and the preservation number of the lactobacillus acidophilus LA4 is CGMCC No.35144; the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain can significantly improve cognitive impairment of an elderly behavior cognitive impairment model mouse and enhance the learning and memory ability of the mouse by reducing deposition of beta amyloid protein in the brain of the mouse, inhibiting the activity of acetylcholin esterase, regulating and controlling the neurotransmitter level, relieving brain inflammatory response, reducing nerve injury, promoting synaptic regeneration and the like. The strain provided by the invention provides a potential treatment scheme for preventing or improving senile behavioral cognitive impairment, and has important development and application values.

The present disclosure relates to compounds of formula (I) that are useful as modulators of α7 nAChR, compositions comprising such compounds, and the use of such compounds for preventing, treating, or ameliorating disease, particularly disorders of the central nervous system such as cognitive impairments in Alzheimer's disease, Parkinson's disease, and schizophrenia, as well as for L-DOPA induced-dyskinesia and inflammation.

Tobacco products comprising smokeless tobacco products and active ingredients, including those that antagonize nicotinic acetylcholine receptors, the TRPV1 channel, and / or the TRPA1 channel are disclosed. Nicotine replacement therapies comprising active ingredients, including those that antagonize nicotinic acetylcholine receptors, the TRPV1 channel, and / or the TRPA1 channel. The active ingredient may reduce or eliminate sensory irritation arising due to use of the product. Analgesic compositions comprising active ingredients. Methods of reducing taste and sensory irritation by employing an active ingredient.

The invention discloses a neuroprotective peptide with anti-inflammatory and anti-oxidation effects and application of the neuroprotective peptide. The neuroprotective polypeptide is a polypeptide with any one of the following sequences: FPDFVYK and APDTRLF. The invention provides an antioxidant effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing antioxidant drugs. The invention provides an anti-inflammatory effect of the neuroprotective polypeptide in a PC12 cell injury model induced by hydrogen peroxide, and the neuroprotective polypeptide can be used for preparing anti-inflammatory drugs. The invention provides the influence of the neuroprotective polypeptide on the expression of pathways and neurotrophic factors in PC12 nerve cells. The neuroprotective polypeptide reduces the activity of acetylcholin esterase (AChE) and up-regulates the expression of neurotrophic factors so as to alleviate nerve cell damage and enhance memory, thereby achieving the purpose of preventing and treating neurodegenerative diseases.

There is provided a composition for topical application to the penis for the treatment of erectile dysfunction, the composition being free of glyceryl trinitrate (GTN), sildenafil and an acetylcholinesterase inhibitor, and comprising volatile and non-volatile solvents, the volatile solvents comprising a lower alcohol and water and the non-volatile solvents comprising a polyhydric alcohol and a glycol. Preferably, the composition does not contain any pharmaceutically active ingredients for the treatment of erectile dysfunction. Also provided is a method of determining the cooling ability of a test composition, such as the composition described above.

The invention discloses a rapid screening and detecting method for vegetable pesticide residues, a multi-dimensional judgment basis is provided for pesticide residue detection by combining a PCR amplification result with the inhibition rate of acetylcholin esterase and butyrylcholin esterase, different types of pesticides may induce different gene expression changes, and the detection result is accurate. According to the rapid screening and detecting method for the pesticide residues in the vegetables, the accuracy of a detection result is improved, meanwhile, the variety of the pesticide residues in the vegetables can be accurately determined, the defect that specific pesticide varieties are difficult to distinguish by purely relying on an enzyme inhibition method is overcome, and the detection result is more targeted and accurate.

The application relates to a preparation method of a biosensor. The application relates to a preparation method of a portable pesticide detection biosensor based on acetylcholinesterase (AChE)@poly(allylamine hydrochloride)-hydrogen bond organic framework (PAH-HOF) and application of the biosensor in on-site rapid chlorpyrifos detection, and belongs to the technical field of biosensors. According to the method, AChE is combined with poly(allylamine hydrochloride) (PAH) to change the surface charge and promote self-assembly of the AChE and the hydrogen bond organic framework (HOF), so that an AChE@PAH-HOF composite material with high catalytic activity, high embedding rate and high stability is formed; then the material is integrated into a water-retaining glycerol-sodium alginate hydrogel (G-SA) matrix to construct a disc-shaped sensor. The sensor can be attached to the surface of plants and used for long-term in-situ monitoring of organophosphorus pesticides. In combination with an image processing algorithm, the system realizes accurate tracking of the chlorpyrifos degradation process on the surface of tomato plants, and the detection limit reaches 1 ng / mL. The application provides non-invasive and low-cost technical support for crop health management and sustainable precision agriculture.

The present application relates to a kind of thieno [2, 3-c] pyridazine derivatives, and its isotope form, stereoisomer, tautomer, cis-trans isomer, pharmaceutically acceptable salt, pharmaceutically acceptable solvate, hydrate, prodrug and polymorph, these compounds can be used as cholinergic M4 receptor positive allosteric modulator, it has good application prospect in preparation for preventing and / or treating the drug for the disease related to abnormal muscarinic acetylcholine receptor.

In alternative embodiments, provided are polyheterocyclic uncharged bisoxime antidotes, including polyheterocyclic uncharged bisoximes, and methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein. In alternative embodiments, provided are methods for treating, preventing or ameliorating excessive acetylcholine stimulation in the brain; or, treating, ameliorating or protecting (preventing) an organophosphate toxicity or poisoning or toxic exposure, or for treating, ameliorating or protecting (preventing) organophosphate inhibition of an acetylcholinesterase (AChE) comprising administering to an individual in need thereof a polyheterocyclic uncharged bisoxime antidote as provided herein.

The invention discloses an application of isothunberine or a pharmaceutically acceptable salt thereof in preparation of a medicine for preventing and / or treating asthma diseases. It is found that in an OVA-induced mouse asthma model, the isothunberine significantly reduces airway resistance excited by acetylcholine, improves inflammatory cell infiltration and airway mucus high secretion of asthma model mouse lung tissue, reduces the levels of lgE and OVA-lgE in serum and related inflammatory factors in alveolar lavage fluid, and has the effect of preventing and treating asthma. Therefore, the effect of treating asthma is achieved. Therefore, the isothunberine has the potential of preventing or treating the asthma, provides a new treatment mode for the treatment of the asthma, and has a wide application prospect.

The present application belongs to the technical field of pharmaceutical therapy, and particularly relates to an amide-substituted tryptophan ketone derivative, a preparation method and application thereof. The structural formula of the tryptophan ketone derivative is shown in formula (A) or formula (B). In the formula, n=1 or 2, and R is any one of dimethylamine group, diethylamine group, morpholine group, 1-methylpiperazine group, cyclopropylamine group and 4-hydroxypiperidyl group. The compound can selectively inhibit the activity of acetylcholinesterase, and has the potential to develop into a drug for treating Alzheimer's disease due to the abilities of self-aggregation and the like. 1‑42 ​

The present invention relates to a compound of formula (I) or a pharmaceutically acceptable salt and / or solvate thereof. The invention also relates to the use of the compounds of formula (I) as a medicament, in particular for the prevention or treatment of obsessive-compulsive disorder and related behaviors, including addiction, obsessive-compulsive disorder and dietary disorder, and neurodegenerative diseases, such as Parkinson's disease or Alzheimer's disease.

The invention relates to the field of medicinal chemistry, and discloses a sulfonyl derivative as well as a preparation method and application thereof. The compound provided by the invention and the composition containing the compound provided by the invention can be used for preparing medicines for diseases mediated by the mushroom type acetylcholine receptor.

The invention provides application of acetylcholine and 1-carboxyl-6-hydroxy-3, 4-dihydro-beta-carboline (the code in the attached drawing of the abstract is CHEMBL461889) in early pregnancy diagnosis, non-targeted metabonomics detection and analysis are carried out on the urine of pregnant and non-pregnant sows by virtue of a liquid chromatography-mass spectrometry technology; the two metabolic markers capable of being used for early pregnancy diagnosis of the sows are screened out by introducing two deep learning models for joint analysis, the AUC values of ROC curves of the two metabolic markers are 1, the accuracy is very high, and the pregnancy state of the sows can be accurately judged only 18 days after the sows are bred. The invention provides a novel effective sow early pregnancy diagnosis method for the pig breeding industry, and has important significance for reducing the breeding cost.

The present invention relates to ophthalmic compositions comprising at least one substance of at least one nicotinic acetylcholine receptor (nAChR) modulator, in particular varenicline, megamine and derivatives thereof, and / or the endogenous Kennedy metabolic pathway, as well as salts and derivatives or pharmaceutically acceptable salts of these substances, and choline-containing phospholipids, salts and derivatives of these substances. From these groups, particularly preferred are choline, phosphocholine, CDP-choline (citicoline), phosphatidylcholine (e.g. Lecithin having a phosphatidylcholine content of > = 80% by weight, e.g. Soybean lecithin), ethanolamine, phosphoethanolamine, CDP-ethanolamine, phosphatidylethanolamine, L-alpha-glycerophosphorylcholine, phosphatidylcholine, phosphatidylethanolamine.

The invention discloses a benzofuran glycoside compound as well as a separation and extraction method, a pharmaceutical composition and application thereof. The compound 1 is separated from an eupatorium chinense extract. And separating and purifying chemical components of the n-butyl alcohol part of the eupatorium chinense root to obtain the benzofuran compound. The inhibition activity of target cyclooxygenase-2 of inflammatory related diseases of all the separated compounds 1 is tested, and the compounds 1 have good inhibition activity on COX-2 enzyme and have good binding energy with target protein. In addition, the compound also has a good NLRP3-related inhibition effect, can be independently used or combined with other medicines to regulate NLRP3-related proteins, and is used for treating NLRP3-related mediated diseases and / or diseases and preparing medicines for preventing or treating the diseases or diseases. The compound 1 also has a good inhibition effect on acetylcholin esterase, and can be used for treating Alzheimer's disease, myasthenia gravis, Parkinson's disease and other diseases.

The application discloses a preparation method of carbon dot nanometer enzyme, which comprises the following steps: dissolving oxalic acid, polyacetyl imine and ferric nitrate in ultrapure water, heating and mixing, cooling to room temperature, centrifuging, filtering, dialyzing and taking supernatant to obtain the carbon dot nanometer enzyme; and a double-mode detection method for colorimetric and fluorescent detection of glyphosate in fruits and vegetables is constructed by using the prepared oxalic acid carbon dot nanometer enzyme, acetylcholinesterase, acetylcholine, 3,3',5,5'-tetramethyl benzidine and H2O2. The oxalic acid carbon dot nanometer enzyme is prepared by doping ferric nitrate and tetraethylenepentamine with oxalic acid as a precursor, has excellent peroxidase-like activity, and a colorimetric and fluorescent double-mode detection method for glyphosate is constructed by using the oxalic acid carbon dot nanometer enzyme, the double-mode detection results can be verified with each other, and the accuracy and reliability of the detection results are significantly improved.

The invention relates to the technical field of cosmetics, in particular to a compound active peptide with functions of resisting aging and whitening skin and application of the compound active peptide. Comprising acetyl hexapeptide-1, nonapeptide-1, acetyl tetrapeptide-11, hexapeptide-9 and palmitoyl hexapeptide-12. The compound peptide disclosed by the invention can play a synergistic anti-aging and whitening role on an epidermal layer and a corium layer, and has multiple advantages; the compound peptide acts on epidermal layer keratinocytes, dermal layer fibroblasts and B16F10 cells, and can be used for detecting the senescence of the cells by influencing the expression quantity of cell senescence marker genes, influencing the cell cycle, influencing the expression quantity of extracellular matrix related proteins, influencing melanin synthesis and influencing the secretion quantity of acetylcholine in the cells. Multiple skin anti-aging and whitening effects synergistically exerted on the epidermal layer and the corium layer are achieved.

The invention provides a phenolic compound with a hangover alleviating effect and a preparation method thereof. The structural formula of the polyphenol compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosylphenyl) ethanone is as shown in formula (I), and the polyphenol compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosylphenyl) ethanone is separated from pueraria thomsonii. In-vitro pharmacological experiments prove that the compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosyl phenyl) ethanone has good acetylcholin esterase inhibitory activity, and the EC50 value of the compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosyl phenyl) ethanone is 0.41 + / -0.07 mM, which indicates that the compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosyl The polyphenol compound 1-(2, 4-dihydroxyphenyl)-2-(4-O-glucosylphenyl) ethanone is expected to be used as a lead compound to develop a novel alcohol effect dispelling and liver protecting medicine.

This invention discloses a method and system for pilot safety risk analysis based on neural computation. The method includes: identifying safety risk factors; establishing a mapping relationship between safety risk factors and unsafe pilot behaviors; establishing a structural model of the cortico-basal ganglia-thalamus neural circuit; establishing AMPA and GABA neural synapse computational models based on the theories of double exponential synapses and α-β synapses, respectively; establishing a computational model of the cortico-basal ganglia-thalamus neural circuit; calculating the thalamic discharge rate under the influence of different safety risk factors, and simulating the regulatory mechanisms of dopamine and acetylcholine during the formation of safety risks. This invention conducts safety analysis from the perspective of pilot neurodynamics, providing key physiological basis and theoretical support for improving risk analysis models and optimizing system design by analyzing the neural regulatory mechanisms of acetylcholine and dopamine during the formation of safety risks.

5, 6, 7, 8-tetrahydro-1, 6-naphthyridines and derivatives thereof, which are substituted in the 6 position with 6, 7-dihydro-5H-pyrrolo [3, 4-b] pyridin-5-one, furo [3, 4-b] pyridin-5 (7H)-one, 6, 7-dihydro-5H-cyclopenta [b] pyridin-5-one, or [1, 2, 4] triazolo [4, 3-a] pyrimidine-3 (2H)-one, are positive allosteric modulators of muscarinic acetylcholine receptor M4 (mAChR M4), and can be used for treating neurological and psychiatric disorders associated with muscarinic acetylcholine receptor dysfunction.

This application relates to the field of medicinal chemistry, specifically disclosing a piperidine derivative, a pharmaceutical composition, its uses, and a method for preparation. The piperidine derivative has the structure of general formula (I). This piperidine derivative can significantly improve the learning and memory abilities of Aβ-induced Alzheimer's disease model rats and effectively reduce acetylcholinesterase activity in brain tissue, providing a potential candidate for the development of novel Alzheimer's disease treatments.

The invention provides a triterpene enantiomer compound as well as a preparation method and application thereof, and relates to the technical field of medicines. The preparation method of the triterpenoid enantiomer compound comprises the following steps: (1) preparing a lycopodium clavatum extract: crushing a dried lycopodium clavatum whole plant, adding the crushed lycopodium clavatum whole plant into an ethanol solution for extraction, collecting an extracting solution, and concentrating the extracting solution under reduced pressure into paste to obtain a lycopodium clavatum crude extract; and (2) separation and purification: adding the crude lycopodium clavatum extract in the step (1) into deionized water for dilution, preparing a suspension, sequentially extracting with petroleum ether and chloroform, concentrating the chloroform extract into extract, and performing column chromatography, thin layer chromatography and molecular sieve chromatography separation to obtain the target triterpenoid enantiomer compounds named (+)-oncerin A (1) and (-)-oncerin A (2). The invention provides a new path for developing a new acetylcholin esterase inhibitor drug, and the application prospect is good.