518 results about "Acetylcholine" patented technology

To provide novel plant environmental stress alleviator and plant growth promoter having an excellent effect of reducing environmental stress such as high temperatures.SOLUTION: Provided is a plant environmental stress alleviator or a plant growth promoter, which contains a plant extract containing acetylcholine or an acetylcholinesterase inhibitor and calcium ions. Also provided is a method for alleviating plant environmental stress or a method for promoting plant growth by applying the plant environmental stress alleviator or the plant growth promoter to a plant.SELECTED DRAWING: None

The invention belongs to the technical field of microorganisms, and particularly relates to a plant lactobacillus B534 strain and application thereof in preparation of functional products beneficial to sleep improvement and emotion regulation. The lactobacillus plantarum B534 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the lactobacillus plantarum B534 is CGMCC No.33031. The lactobacillus plantarum B534 is named as lactobacillus plantarum B534. The plant lactobacillus B534 provided by the invention is a natural strain and has food safety; the lactobacillus plantarum B534 has relatively strong acetylcholin esterase inhibition activity, and also has relatively strong capability of synthesizing and secreting GABA (gamma-aminobutyric acid), 3-HB (3-hydroxybutyric acid) and 5-HT (5-hydroxytryptamine); meanwhile, the plant lactobacillus B534 has relatively strong acid resistance and cholate resistance and also has relatively strong tolerance to simulated gastrointestinal fluid, so that the plant lactobacillus B534 has relatively good intestinal colonization ability and has important application value in the fields of products beneficial to sleep improvement, emotion regulation products and the like.

The invention belongs to the technical field of monatomic nano-enzyme material preparation, and particularly relates to a nano-enzyme preparation method for acetylcholin esterase activity detection. The Ni-NiO heterojunction material is successfully synthesized by adopting Ni-MOF as a carrier and accurately regulating and controlling the ratio of nitrogen to air in the ultrafast Joule hot calcination process. The material has rich oxygen vacancies and metal Ni sites, the oxygen vacancies and the metal Ni sites can effectively serve as anchoring sites of Pt single atoms, the loading capacity and dispersity of the Pt single atoms are remarkably improved, and therefore the excellent nano-enzyme catalytic activity is shown. In addition, the preparation method disclosed by the invention is simple and convenient in process and has a wide industrial application prospect. An acetylcholin esterase activity colorimetric sensing method constructed on the basis of the nanometer enzyme for acetylcholin esterase activity detection prepared by the method has extremely high sensitivity and low detection limit. The detection limit of the technology can be as low as 0.037 mU / mL, the problem that the sensitivity of a traditional detection method in a low-concentration sample is insufficient is remarkably solved, and remarkable clinical application potential is shown.

The invention belongs to the field of drug preparation, and particularly relates to a preparation method and application of a drug delivery system based on hollow mesoporous polydopamine nanoparticles. The drug delivery system comprises hollow mesoporous polydopamine nanoparticles, a drug loaded inside and / or outside the hollow mesoporous polydopamine nanoparticles, and pectin wrapping the hollow mesoporous polydopamine nanoparticles, the medicine is a medicine with a nerve protection effect and an acetylcholin esterase inhibitor. The drug delivery system can reduce proinflammatory cytokines, regulate functions of target immune cells, synergistically reduce necrosis of neurons in intestinal tracts and induce neural crest stem cells in the intestinal tracts to be differentiated into neurons.

Disclosed herein are substituted hexahydro-1H-cyclopenta[c]pyrrole compounds, which may be useful as antagonists of the muscarinic acetylcholine receptor M4 (mAChR M4). Also disclosed herein are methods of making the compounds, pharmaceutical compositions comprising the compounds, and methods of treating disorders using the compounds and compositions.

The invention discloses parabacteroides faecium capable of relieving senescence and application of the parabacteroides faecium, and belongs to the technical field of microorganisms and the technical field of medicines. Animal experiments prove that Parabacteroides vervae CCFM1428 has the effects of improving the weakness index of a mouse, increasing peroxide dismutase in the liver of the mouse, reducing malonaldehyde, increasing the level of glutathione peroxidase (GSH-px), increasing the level of catalase, reducing an inflammatory factor IL-6, reducing an inflammatory factor TNF-alpha and increasing the level of acetylcholine in the brain of the mouse, and has the effects of improving the weakness index of the mouse, increasing the liver peroxide dismutase of the mouse, reducing malonaldehyde, increasing the level of glutathione peroxidase (GSH-px) and the level of catalase. The gripping force of the mouse is improved. The strain disclosed by the invention has a huge application prospect in preparation of products for relieving senescence.

The invention relates to a preparation method of an effective part extract of n-butyl alcohol of Eupatorium chinense and application of the effective part extract in treatment of diabetes complicated with depression. The technical scheme of the invention is to provide an extract of the n-butyl alcohol effective part of the eupatorium chinense, which can be used for treating diabetes complicated by depression diseases. In-vitro experiments show that the eupatorium chinense extract has an inhibition effect on acetylcholin esterase (AChE). In-vivo animal experiments find that classical anti-diabetes and depression treatment drugs, namely metformin hydrochloride and fluoxetine hydrochloride, are taken as positive controls, experimental research on the treatment effect of the effective part extract of the n-butyl alcohol of the eupatorium chinense on mice suffering from diabetes complicated with depression is developed, and research results show that the extract can regulate insulin resistance and can be used for treating diabetes complicated with depression. The glycometabolism of mice with diabetes mellitus complicated with depression is improved, and the depression symptom is improved. The extract has a remarkable effect of treating diabetes mellitus complicated with depression, has a good effect of treating diabetes mellitus complicated with depression, and is expected to be developed into a new natural medicine for treating diabetes mellitus complicated with depression.

The invention provides an RPA-CRISPR-Cas12a system-based detection reagent for the drug resistance of a chilo suppressalis organophosphorus insecticide, a detection method and application, and belongs to the technical field of molecular detection. The invention provides a detection reagent for chilo suppressalis organophosphorus insecticide resistance, the reagent comprises a specific RPA2 primer pair designed for chilo suppressalis acetylcholin esterase ace-1 gene A314S resistance mutation site and crRNA, a double screening mechanism of RPA amplification and CRISPR-Cas12a is combined, wild type and mutant type genes can be accurately distinguished, and the detection reagent can be used for detecting the insecticide resistance of the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis organophosphorus insecticide resistance to the chilo suppressalis. The rapid, sensitive and visual nucleic acid detection of the chilo suppressalis ace-1 gene A314S resistance mutation site is realized, and a visual detection means which is high in specificity, simple and convenient to operate, economical and efficient is provided for field on-site determination of chilo suppressalis resistance populations.

Deuterium-labeled 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidines substituted with pyrrolo[3,4-b]pyridin-5-one, furo[3,4-b]pyridin-5(7H)-one, or [1,2,4]triazolo[4,3-a]pyrimidin-3(2H)-one are positive allosteric modulators of the muscarinic acetylcholine receptor M4 (mAChR M4) and may have use in treating neurological and psychiatric disorders associated with muscarinic acetylcholine receptor dysfunction.

Deuterium-labeled 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidines substituted with pyrrolo[3,4-b]pyridin-5-one, furo[3,4-b]pyridin-5(7H)-one, or [1,2,4]triazolo[4,3-a]pyrimidin-3(2H)-one are positive allosteric modulators of the muscarinic acetylcholine receptor M4 (mAChR M4) and may have use in treating neurological and psychiatric disorders associated with muscarinic acetylcholine receptor dysfunction.

The invention discloses a preparation method of a carbon dot nano-enzyme, which comprises the following steps: dissolving oxalic acid, polyethyleneimine and ferric nitrate in ultrapure water, heating, mixing and reacting, cooling to room temperature, centrifuging, filtering, dialyzing, and taking supernate to obtain the carbon dot nano-enzyme. The prepared oxalic acid carbon dot nano-enzyme is matched with acetylcholin esterase, acetylcholine, 3, 3 ', 5, 5'-tetramethyl benzidine and H2O2 to construct a colorimetric and fluorescent dual-mode detection method for glyphosate in fruits and vegetables. The oxalic acid carbon dot nano-enzyme is prepared by doping ferric nitrate and tetraethylenepentamine into oxalic acid serving as a precursor, the oxalic acid carbon dot nano-enzyme has excellent peroxidase-like activity, a colorimetric and fluorescent dual-mode detection method of glyphosate is constructed through the oxalic acid carbon dot nano-enzyme, dual-mode detection results can be mutually verified, and the detection result is accurate. And the accuracy and the reliability of a detection result are remarkably improved.

Provided herein are compounds which are useful as antagonists of the muscarinic acetylcholine receptor M1 (mAChR M1); synthetic methods for making the compounds; pharmaceutical compositions comprising the compounds; and methods of treating neurological and psychiatric disorders associated with muscarinic acetylcholine receptor dysfunction using the compounds and compositions.

The invention belongs to the technical field of biological information, and relates to an acetylcholin esterase inhibitor prediction method based on Stacking ensemble learning and molecular feature fusion, which comprises the steps of data collection and preparation, data annotation and optimization, feature extraction and analysis, construction of a Stacking model, result verification and feedback and construction of a prediction platform. The molecular fingerprints and the property descriptors are used as features, and an acetylcholin esterase inhibitor classifier is successfully constructed by adopting a Stacking algorithm. According to the method, the problems that the efficiency of finding the acetylcholin esterase inhibitor by a traditional experimental method is low, and a common quantitative structure-function relationship method is high in complexity and poor in generalization ability can be solved, the new drug finding speed is increased, experimental candidates are accurately positioned, and resource waste is reduced.

Deuterium-labeled 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidines substituted with pyrrolo[3,4-b]pyridin-5-one, furo[3,4-b]pyridin-5(7H)-one, or [1,2,4]triazolo[4,3-a]pyrimidin-3(2H)-one are positive allosteric modulators of the muscarinic acetylcholine receptor M4 (mAChR M4) and may have use in treating neurological and psychiatric disorders associated with muscarinic acetylcholine receptor dysfunction.

The invention discloses preparation and application of an arundina graminifolia fermentation extract. The arundina graminifolia is fermented by adopting saccharomyces cerevisiae, so that the yield of total polyphenol (increased by 119.1%) and the yield of flavone (increased by 43.8%) in an arundina graminifolia extracting solution can be remarkably increased. The content of soluble solids in the obtained fermentation broth is 9.12 mg / mL, and the fermentation broth has acetylcholin esterase inhibitory activity (IC50 is 4.0 mg / mL) and oxidation resistance (the free radical scavenging rates IC50 of ABTS and DPPH are 0.116 mg / mL and 16.2 [mu] g / mL respectively); the fermentation liquor is high in sensory evaluation and palatability, and can be applied to nerve health maintenance in the field of homology of medicine and food as well as antioxidant functional beverages and health care products.

Deuterium-labeled 4-((2,3-dihydrobenzo[b][1,4]dioxin-6-yl)oxy)piperidines substituted with pyrrolo[3,4-b]pyridin-5-one, furo[3,4-b]pyridin-5(7H)-one, or [1,2,4]triazolo[4,3-a]pyrimidin-3(2H)-one are positive allosteric modulators of the muscarinic acetylcholine receptor M4 (mAChR M4) and may have use in treating neurological and psychiatric disorders associated with muscarinic acetylcholine receptor dysfunction.

The invention belongs to the technical field of synthetic chemistry, and provides a muscarinic acetylcholine receptor 3 fluorescent probe as well as a preparation method and application thereof. The chemical structural formula of the fluorescent probe is shown in the specification, and the fluorescent probe can be used for M3 receptor tracing in cells or organisms. The probe provided by the invention has a good fluorescent property and can be influenced by the polarity and viscosity of an external environment; and the probe has good biocompatibility, and the IC50 of the probe is 43 times of the concentration of a working solution, so that the probe can meet the requirements of cell fluorescence imaging. In the aspect of cell fluorescence imaging, the probe can realize selective fluorescence labeling of the M3 receptor in the cell, washing is not needed in the dyeing process, and the operation is simple and convenient, so that visual research on positioning of the M3 receptor in the cell is realized.

The invention provides a biological enzyme preparation for rapidly reducing pesticide residues and a preparation method thereof, and belongs to the technical field of pesticide residue degradation. The invention provides a biological enzyme preparation capable of rapidly reducing pesticide residues. The biological enzyme preparation is prepared from the following components in parts by weight: 10 to 20 parts of phytase, 10 to 50 parts of acetylcholin esterase, 5 to 10 parts of protease, 10 to 20 parts of tyrosinase, 1 to 3 parts of radix platycodonis extract and 1 to 3 parts of aloe extract. According to the biological enzyme preparation capable of rapidly and efficiently reducing the pesticide residues, the removal rate of organophosphorus pesticides on fruits and vegetables is 95% or above, the removal rate of pyrethroid pesticides on the fruits and vegetables is 70% or above, and the biological enzyme preparation is simple in formula, easy to clean and free of secondary residues.

The invention relates to the field of microorganisms, and provides an acid-producing propionibacterium BL8 with hypoglycemic and anti-aging functions and application of the acid-producing propionibacterium BL8, the acid-producing propionibacterium BL8 is preserved in China General Microbiological Culture Collection Center on December 20, 2024, and the preservation number is CGMCC No. 33134. The acid-producing propionibacterium BL8 disclosed by the invention can be used for inhibiting the activity of DPP4, promoting insulin secretion of INS-1, and inhibiting the activity of acetylcholin esterase and excellent cellulase activity. The blood sugar can be reduced; the memory is improved, and the Alzheimer disease is prevented and relieved; and the traditional Chinese medicine composition has relatively high research and application values in the aspects of reducing blood sugar and resisting aging.

The invention belongs to the technical field of functional grease processing, and particularly relates to an enzymolysis preparation method and application of diglyceride with an effect of improving Alzheimer's disease. The method comprises the following steps: mixing low-erucic acid rapeseed oil, glycerol and water, and heating to obtain a pre-reaction mixture; adding compound enzyme for reaction, and then adding candida antarctica lipase B and pseudomonas fluorescens lipase for reaction; and after the reaction is completed, carrying out molecular distillation, collecting a light-phase product, carrying out adsorption filtration, and collecting filtrate to obtain the diglyceride oil. The compound enzyme is composed of rhizomucor miehei lipase, candida rugosa lipase and candida antarctica lipase B in a weight ratio of (2-4): 2: 1. According to the method, the content of diglyceride is effectively increased, the prepared composition has the efficacy of preventing and treating Alzheimer's disease, and the efficacy is shown as improvement of reaction capacity and cognitive function, inhibition of brain apoptosis and acetylcholin esterase, enhancement of SOD and CAT vitality and improvement of related gene expression.

The invention provides therapeutic methods, pharmaceutical compositions, and unit dose formulations for treating movement disorders, such as using a muscarinic acetylcholine receptor inhibitor in combination with a muscarinic acetylcholine receptor activator to treat dystonia. In some aspects, the invention provides a method of treating a movement disorder in a patient, where the method comprises administering to a patient in need thereof (i) a muscarinic acetylcholine receptor inhibitor in an amount effective to treat the movement disorder and (ii) a muscarinic acetylcholine receptor activator in an amount effective to reduce the frequency and / or magnitude of at least one side effect of the muscarinic acetylcholine receptor inhibitor, to thereby treat the movement disorder.

This invention discloses a protein and gene related to resistance to neonicotinoid acetylcholine receptor competitive regulator insecticides in peach aphids, and their applications. It relates to the field of screening technology for neonicotinoid acetylcholine receptor competitive regulator insecticides in peach aphids. This invention obtains and clarifies an MpeABCC_4G protein and its encoding gene that is closely related to the resistance of peach aphids to neonicotinoid acetylcholine receptor competitive regulator insecticides. By knocking down the expression of MpeABCC_4G in resistant peach aphids using RNAi technology, the control efficacy of neonicotinoid acetylcholine receptor competitive regulator insecticides is significantly improved.

This invention belongs to the field of microbial technology, specifically relating to a strain of *Pediococcus pentosaceus* SUN02 with acetylcholinesterase inhibitory activity and its applications. This *Pediococcus pentosaceus* SUN02 is deposited at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCC No. 29705. The *Pediococcus pentosaceus* SUN02 provided by this invention exhibits strong acid and bile salt resistance, good tolerance in simulated gastric and intestinal fluids, and can successfully reach the human intestine. *Pediococcus pentosaceus* SUN02, in its bacterial suspension and inactivated cells, possesses strong acetylcholinesterase inhibitory activity, produces γ-aminobutyric acid (GABA), and has antioxidant and other probiotic functions. Its application in probiotic products may help improve Alzheimer's disease, enhance learning and memory abilities, regulate mood, and improve sleep.

Described herein are compounds that reactivate acetylcholinesterase and associated methods of treating organophosphate poisoning.

The invention belongs to the technical field of microorganisms, and relates to lactobacillus acidophilus LA4 and application thereof. The classification name of the lactobacillus acidophilus LA4 is lactobacillus acidophilus, the lactobacillus acidophilus LA4 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on July 9, 2025, and the preservation number of the lactobacillus acidophilus LA4 is CGMCC No.35144; the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The strain can significantly improve cognitive impairment of an elderly behavior cognitive impairment model mouse and enhance the learning and memory ability of the mouse by reducing deposition of beta amyloid protein in the brain of the mouse, inhibiting the activity of acetylcholin esterase, regulating and controlling the neurotransmitter level, relieving brain inflammatory response, reducing nerve injury, promoting synaptic regeneration and the like. The strain provided by the invention provides a potential treatment scheme for preventing or improving senile behavioral cognitive impairment, and has important development and application values.

The present disclosure relates to compounds of formula (I) that are useful as modulators of α7 nAChR, compositions comprising such compounds, and the use of such compounds for preventing, treating, or ameliorating disease, particularly disorders of the central nervous system such as cognitive impairments in Alzheimer's disease, Parkinson's disease, and schizophrenia, as well as for L-DOPA induced-dyskinesia and inflammation.

The invention discloses an anti-wrinkle composition containing acetyl hexapeptide-8 as well as a preparation method and application of the anti-wrinkle composition. The anti-wrinkle composition comprises polypeptide lipidosome, a stevia rebaudiana extract, a stevia rebaudiana leaf / stem extract and a paullinia cupana seed extract, the polypeptide lipidosome comprises a lipid membrane and polypeptide located in the lipid membrane, and the polypeptide comprises acetyl hexapeptide-8, copper peptide and nonapeptide-1. The acetyl hexapeptide-8 is used for reducing the release of acetylcholine so as to effectively relax muscles, and a good instant anti-wrinkle effect is achieved; and copper peptide and nonapeptide-1 are supplemented to promote the expression of collagen and the like, stimulate the regeneration of a corium layer structure and bring a good long-acting anti-wrinkle effect. Meanwhile, the polypeptide combination is delivered by adopting lipidosome, so that the transdermal performance and the long-acting anti-wrinkle effect of the polypeptide combination are enhanced. According to the anti-wrinkle composition, the stevia rebaudiana leaf / stem extract serves as an instant anti-wrinkle reinforcing component, the stevia rebaudiana leaf / stem extract is matched with the copper peptide and the nonapeptide-1, a better collagen stimulation effect is achieved, the long-acting anti-wrinkle effect of the composition is enhanced, and the paullinia cupana cacao seed extract can strengthen skin barriers and improve the skin compactness.

The invention belongs to the technical field of medicines, and particularly relates to a triterpenoid extracted from edible and medicinal fungus ganoderma lucidum, a preparation method of the triterpenoid and application of the triterpenoid in the aspect of resisting Alzheimer's disease. The triterpenoid compound is shown as a structural formula I. The triterpenoid compound provided by the invention has remarkable acetylcholin esterase inhibitory activity, is an ideal candidate compound of an acetylcholin esterase inhibitor which is novel in structure and efficient in activity, and has a good application prospect in the aspect of treatment of Alzheimer's disease. # imgabs0 #

Tobacco products comprising smokeless tobacco products and active ingredients, including those that antagonize nicotinic acetylcholine receptors, the TRPV1 channel, and / or the TRPA1 channel are disclosed. Nicotine replacement therapies comprising active ingredients, including those that antagonize nicotinic acetylcholine receptors, the TRPV1 channel, and / or the TRPA1 channel. The active ingredient may reduce or eliminate sensory irritation arising due to use of the product. Analgesic compositions comprising active ingredients. Methods of reducing taste and sensory irritation by employing an active ingredient.

The present invention provides an anti-aging combination peptide and its application, which is composed of 0.1-1% palmitoyl tripeptide-5, 0.5-5% palmitoyl pentapeptide-4, 1-10% acetyl hexapeptide-8, and 0.5-5% hexapeptide-9. Since hexapeptide-9 is added to the original three polypeptides of palmitoyl tripeptide-5, palmitoyl pentapeptide-4, and acetyl hexapeptide-8, and a suitable ratio is adopted, this combination can significantly promote the expression of collagen I, collagen III, and collagen IV genes, significantly improve the effect of the combination peptide in increasing collagen content, provide a theoretical basis for the production of collagen in the skin, and can also inhibit the secretion of acetylcholine and reduce the generation of dynamic lines, thereby having a better anti-aging and wrinkle-removing effect on the skin.