285 results about "Carbonic anhydrase" patented technology

Disclosed herein among other MN / CA IX-related inventions are new MN / CA IX-specific antibodies generated from MN / CA IX-deficient mice, preferably monoclonal antibodies and immunoreactive fragments and engineered variants thereof. Subsets of the new antibodies are to either the proteoglycan-like (PG) domain or to the carbonic anhydrase (CA) domain of MN / CA IX, and methods are provided by which antibodies can be prepared to the other MN / CA IX domains. Such new MN / CA IX-specific antibodies, fragments and variants are useful diagnostically / prognostically and therapeutically for cancer and precancer. Particularly preferred are the new monoclonal antibodies, fragments and variants that are specific for the non-immunodominant epitopes of MN / CA IX, which antibodies are, among other uses, useful to detect soluble MN / CA IX (s-CA IX) in body fluids, alone but preferably in combination with antibodies specific to the immunodominant epitopes of MN / CA IX, for example, in a sandwich assay.

Disclosed herein is the discovery of a soluble MN / CA IX (s-CA IX) in body fluids, such as, urine and serum. Said s-CA IX comprises the extracellular domain of CA IX or portions thereof. The predominant s-CA IX species is the extracellular domain comprising a proteoglycan-like (PG) domain and carbonic anhydrase (CA) domain, and having a molecular weight of about 50 / 54 kilodaltons (kd) upon Western blot. A smaller s-CA IX form of about 20 to about 30 kd comprising the CA domain or parts thereof, not linked to the PG domain, has also been found in body fluids. Diagnostic / prognostic methods for precancer and cancer that detect or detect and quantitate said s-CA IX in body fluids, are described. Also disclosed herein is the coexpression of CA IX and HER-2 / neu / c-erbB-2 that provides parallel, alternative and potentially synergistic diagnostic / prognostic and therapeutic strategies for precancer and cancer. Further disclosed are new MN / CA IX-specific antibodies generated from MN / CA IX-deficient mice, preferably monoclonal antibodies and immunoreactive fragments and engineered variants thereof. Such new MN / CA IX-specific antibodies, fragments and variants are useful diagnostically / prognostically and therapeutically for cancer and precancer. Particularly preferred are the new monoclonal antibodies, fragments and variants that are specific for the non-immunodominant epitopes of MN / CA IX, which antibodies are, among other uses, useful to detect soluble MN / CA IX (s-CA IX) in body fluids, alone but preferably in combination with antibodies specific to the immunodominant epitopes of MN / CA IX, for example, in a sandwich assay.

Disclosed herein is the discovery of a soluble MN / CA IX (s-CA IX) in body fluids, such as, urine and serum. Said s-CA IX comprises the extracellular domain of CA IX or portions thereof. The Predominant s-CA IX species is the extracellular domain comprising a proteoglycan-like (PG) domain and carbonic anhydrase (CA) domain, and having a molecular weight of about 50 / 54 kilodaltons (kd) upon Western blot. A smaller s-CA IX form of about 20 to about 30 kd comprising the CA domain or parts thereof, not linked to the PG domain, has also been found in body fluids. Diagnostic / prognostic methods for precancer and cancer that detect or detect and quantitate said s-CA IX in body fluids, are described. Also disclosed herein is the coexpression of CA IX and HER-2 / neu / c-erbB-2 that provides parallel, alternative and potentially synergistic diagnostic / prognostic and therapeutic strategies for precancer and cancer. Further disclosed are new MN / CA IX-specific antibodies generated from MN / CA IX-deficient mice, preferably monoclonal antibodies and immunoreactive fragments and engineered variants thereof. Such new MN / CA IX-specific antibodies, fragments and variants are useful diagnostically / prognostically and therapeutically for cancer and precancer. Particularly preferred are the new monoclonal antibodies, fragments and variants that are specific for the non-immunodominant epitopes of MN / CA IX, which antibodies are, among other uses, useful to detect soluble MN / CA IX (s-CA IX) in body fluids, alone but preferably in combination with antibodies specific to the immunodominant epitopes of MN / CA IX, for example, in a sandwich assay.

The invention discloses a method for repairing a crack of a cement-based material. According to the method, carbonic anhydrase microorganisms are adopted for generating carbonic anhydrase used for catching CO2, CO2 is promoted to be converted into CO3<2->, and the deposition of calcium carbonate within a surface area of the crack is accelerated; the method has the advantage that the repair speed is faster than that of other methods for repairing the crack through the microorganisms. The method comprises the following steps of: inoculating bacillus mucilaginosus to a culture medium; culturing; preparing bacillus mucilaginosus concentrated bacteria liquid; immobilizing bacteria on carriers; uniformly burying calcium sources into the cement-based material during forming the cement-based material; concentrating the carriers with the bacteria immobilized into a range of 5mm under a surface area of a test part; manufacturing the crack after a part to be tested is cured; transferring into a thermostatic waterbath; continuously charging air for maintaining and repairing. The repair test result shows that the osmotic coefficient is greatly reduced after repairing for 5 days, and the crack is repaired completely after continuously repairing for 30 days.

A process is disclosed for gas separation wherein carbon dioxide in a mixed gas stream is converted to bicarbonate by contacting a gamma carbonic anhydrase enzyme designated as CAM in the temperature range of 40 degrees to 85 degrees C. in an enzyme catalyzed carbon dioxide capture system.

The present invention relates to a reactor and a process suitable for extracting carbon dioxide from carbon dioxide-containing gas stream. The reactor is based on a two module system where absorption occurs in one module and desorption occurs in the other module. The absorption and desorption modules in the system include at least one gas-liquid membrane (GLM) module and at least one direct gas-liquid contact (DGLC) module. The carbon dioxide extraction may be catalyzed by carbonic anhydrase.

There is provided a microchip-based differential-type carbon dioxide gas sensor for detecting dissolved carbon dioxide levels. It functions with at least one working electrode composed of an unbuffered hydrogel membrane containing a certain amount of sodium bicarbonate and a pH-sensitive gas-permeable membrane; and a reference electrode composed of a buffered hydrogel membrane and a pH-sensitive gas-permeable membrane. The unbuffered hydrogel membrane contains carbonic anhydrase, which reduces the time period for the hydration of carbon dioxide, thereby allowing the quick measurement of the level of carbon dioxide. In addition to being significantly improved in stabilization, sensing, and recovering time periods, the differential-type carbon dioxide gas sensor can be fabricated in small sizes and quickly measure levels of carbon dioxide dissolved in sample solution.

The present invention relates to a reactor and a process suitable for extracting carbon dioxide from carbon dioxide-containing gas stream. The reactor is based on a two module system where absorption occurs in one module and desorption occurs in the other module. The carbon dioxide extraction may be catalyzed by carbonic anhydrase.

The present invention provides a resin-wafer electrodeionization (RW-EDI) apparatus including cathode and anode electrodes separated by a plurality of porous solid ion exchange resin wafers, which when in use are filled with an aqueous fluid. The apparatus includes one or more wafers comprising a basic ion exchange medium, and preferably includes one or more wafers comprising an acidic ion exchange medium. The wafers are separated from one another by ion exchange membranes. The fluid within the acidic and / or basic ion exchange wafers preferably includes, or is in contact with, a carbonic anhydrase (CA) enzyme to facilitate conversion of bicarbonate ion to carbon dioxide within the acidic medium. A pH suitable for exchange of CO2 is electrochemically maintained within the basic and acidic ion exchange wafers by applying an electric potential across the cathode and anode.

A method for quantifying a neurodegenerative disorder in a patient that includes obtaining a fluid sample from the subject; measuring a protein biomarker complex in said fluid sample and correlating the measurement with mild cognitive impairment or Alzheimer's disease status. The biomarkers include those that comprise at least one of a transthyretin protein and / or a prostaglandin-H2 D-isomerase protein, and at least one second, different protein selected from a transthyretin, prostaglandin-H2 D-isomerase, beta-2-microglobulin, cystatin C, superoxide dismutase [Cu—Zn], plasma retinol-binding protein, phosphatidylethanolamine-binding protein, carbonic anhydrase 2, prostaglandin-H2 D-isomerase, and / or serotransferrin protein.

A method for quantifying a neurodegenerative disorder in a patient that includes obtaining a fluid sample from the subject; measuring a protein biomarker complex in said fluid sample and correlating the measurement with mild cognitive impairment or Alzheimer's disease status. The biomarkers include those that comprise at least one of a transthyretin protein and / or a prostaglandin-H2 D-isomerase protein, and at least one second, different protein selected from a transthyretin, prostaglandin-H2 D-isomerase, beta-2-microglobulin, cystatin C, superoxide dismutase [Cu—Zn], plasma retinol-binding protein, phosphatidylethanolamine-binding protein, carbonic anhydrase 2, prostaglandin-H2 D-isomerase, and / or serotransferrin protein;

The invention provides a method for preparing calcium carbonate by using catalysis of microbial carbonic anhydrase, which comprises the following steps of: adding the carbonic anhydrase extracted from a microbial culture into a liquid system containing calcium ion and bicarbonate radical ion solution or a gas diffusion system containing calcium ion solution and releasing CO2 by using ammonium hydrogen carbonate solution, performing oscillating reaction at room temperature, controlling initial pH at 5 to 10, taking out the produced sediment, and performing filtration, washing and drying to obtain the calcium carbonate. The method makes full use of microbial resources and characteristics of specificity and high efficiency of enzyme catalytic reaction, prepares the calcium carbonate by accelerated deposition, and has the advantages of riche resources, simple process, clean environment, low cost, high efficiency, quickness and the like. The calcium carbonate prepared by deposition has high purity, is calcite crystal calcium carbonate, can be used as an industrial filler, and can also be used for repairing cracks of building engineering, buildings, stone landscapes and carved stone cultural relics.

The invention provides agents that target carbonic anhydrase, which can be used as imaging agents or therapeutic agents. The agents can be used to image tumor hypoxia as well as other physiological processes in a subject.

There is provided a panel of biomarkers of tumour metastasis comprising any two of carbonic anhydrase-9 (CAIX), vascular endothelial growth factor C (VEGF-C), ephrin A5 (EFNA5), eph receptor B2 (EPHB2), transforming growth factor beta 3 (TGF-β3), pyruvate dehydrogenase kinase isoenzyme-3 (PDK3), carbonic anhydrase-12 (CAXII), keratin 14 (KRT14), hypoxia inducible factor 1 alpha subunit (HIF-1α), or tenascin C (TNC). CAIX, VEGF-C, EFNA5, EPHB2, TGF-β3 or PDK3 may be indicators of moderate metastatic potential, while CAXII, KRT14, HIF-1α, or TNC may be indicators of high metastatic potential. There is also provided a method of determining risk of tumour metastasis using the aforementioned biomarkers is also provided. The biomarkers may be used in diagnosis, prognosis, treatment selection, or to test putative therapeutics. The biomarkers may be used to assess malignancies or cancers having hypoxic regions, such as breast cancer.

The invention provides a preparation method of a nano carrier for tumor photo-dynamics therapy (PDT). According to the preparation method, Fe<3+> salts are dissolved in DMF, then a photo-sensitizer (TCPP) is added to obtain particles (NMOFs); then the particles are dispersed in water under the assistance of ultrasonic waves; crosslinking agents (EDC and NHS) are added; after the reactions betweenBSA and sulfadiazine (SDs) finish completely, dialysis is performed to obtain particles (NMOFs@BSA / SDs); then the particles are dispersed in distilled water, then Mn<2+> salts are added, the pH is adjusted, and finally dialysis is performed to obtain a carrier (NMOFs@BSA / SDs@MnO2). Nano metal organic framework particles are taken as the basis and coated by protein (BSA) and sulfadiazine (SDs); finally the particles are in-situ mineralized to obtain required particles; SDs can specifically recognize carbonic anhydrase of tumors and is capable of actively targeting the oxygen-deficient parts oftumors; MnO2 generated in mineralization can catalyze the H2O2 decomposition to increase the oxygen content of tumors; and the PDT efficiency is improved.

The invention belongs to the technical field of biology and relates to a protein chip reagent kit and a method for comprehensively detecting a lung cancer marker. The protein chip reagent kit for comprehensively detecting the lung cancer marker comprises: (1) a chip (1) and (2) a reaction agent and a detection agent, wherein a plurality of specific antibodies are simultaneously fixed on the chip, can generate antibody-antigen reaction with the lung cancer marker and are fixed on a bottom film of the chip to form a plurality of independent recognition sites; and the reaction agent and the detection agent are used for detecting whether a matter capable of generating antibody-antigen reaction with the specific antibodies exists in a sample to be detected or not through a TRFIA (Time Resolved Fluorescence Immunoassay) method. The reagent kit and the method have the beneficial effects that the six indexes of NSE (Neuron Specific Enolase), SCC (Squamous Cell Carcinoma), CEA (Carcino Embryonie Antigen), CA (Carbonic Anhydrase) 19-9, CYFR (Cytokeratin Fragment) A21-1 and pro-GRP (Glass Reinforced Plastic) can be simultaneously detected, the detection accuracy is improved, the repetitive experimental steps are reduced, and the time and the cost are saved.

Disclosed is a formulation for the absorption of CO2, which comprises water, at least one CO2 absorption compound and a carbonic anhydrase as an activator to enhance the absorption capacity of the CO2 absorption compound. The invention also concerns the use of carbonic anhydrase, in a CO2 absorption solution to increase the CO2 absorption rate of such solution.

The invention relates to a method for preparing calcium carbonate by simultaneous mineralization of two microorganisms. The method is characterized by comprising the following steps: adding a culturesolution of the two cultured microorganisms to a mixed solution of calcium salt and urea, putting a reactor in an open mode to enable the solution to be exposed to the air to be subjected to mineralization reaction, taking out the precipitate after the reaction is completed, and washing and drying the precipitate to obtain a calcium carbonate product. The two microorganisms are respectively sporosarcina pasteurii belonging to a urease high-yield strain and bacillus pumilus belonging to a carbonic anhydrase high-yield strain. The method provided by the invention has the advantages that the twostrains together participate in the mineralization process, the generation efficiency of calcium carbonate is increased, simultaneously, the pollution to the environment caused by generation of ammonia gas can be reduced, CO2 in the atmosphere can also be captured, and the advantages of the two strains are complementary so as to cooperatively promote the whole mineralization process; the microorganisms utilized in the method are rich in resources, efficient and single in enzyme catalysis reaction, simple in production process and low in cost; and furthermore, the obtained calcium carbonate product is higher in purity, has better cementing action and can be widely applied to the field of civil engineering.

The invention discloses a method and device for immobilizing CO2 in industrial tail gas by enhancing mineral carbonation. The device comprises a packed column absorber, a mineral leaching tank, a carbonation reactor and a belt type filter. The method comprises the steps of: enabling the industrial tail gas containing CO2 to enter the packed column absorber filled with immobilization carbonic anhydrase, rapidly transforming the CO2 into HCO3<-> under the catalysis action of the carbonic anhydrase; with a weak acidic solution containing the HCO3<-> as a mineral leaching agent, effectively leaching calcium ions from minerals in the mineral leaching tank under the action of ultrasonic waves to form a slurry enriching Ca<2+>; introducing the slurry enriching Ca<2+> into the carbonation reactor, adding a calcium-containing alkali material, regulating the pH of the slurry to be 7-9 to ensure that the HCO3<-> is transformed into CO3<2->, and generating a carbonation reaction with the Ca<2+> leached from the minerals to generate CaCO3. The invention can promote the CO2 to be rapidly transformed into the HCO3<->, can promote the calcium ions to be leached from the minerals and the carbonation reaction, and further realizes that the CO2 in the industrial tail gas is immobilized through direct carbonation under normal pressure.

The present invention relates to an improved process for the preparation of 5,6-dihydro-4-(S)-(ethylamino)-6-(S)methyl-4H-thieno[2,3b]thiopyran-2-sulphonamide-7,7-dioxide hydrochloride of formula (I) commonly known as Dorzolamide Hydrochloride useful as an agent to reduce intraoccular pressure by inhibiting carbonic anhydrase enzyme

Novel radiopharmaceuticals that are useful in diagnostic imaging and therapeutic treatment of disease characterized by over expression of CA-IX comprise a complex that contains a sulfonamide moiety which is capable of binding the active catalytic site of CA- IX, and a radionuclide adapted for radioimaging and / or radiotherapy.

The invention discloses a test kit of auxiliary diagnosis of non-small cell lung cancer patients. The test kit comprises a product used for detecting protein landmark isocitrate dehydrogenase 1(IDH1), a product used for detecting protein landmark carbonic anhydrase 125 (CA125), a product used for detecting protein landmark CYFRA21-1 and a carrier on which a functional expression p=1 / (1+(20.186*0.406<x1>*0.923<x2>*0.656<x3>)<-1>) is recorded, wherein x1 represents the concentration of IDH1, x2 represents the concentration of CA125, and x3 represents the concentration of CYFRA21-1. The test kit is adopted and the non-small cell lung cancer patients are diagnosed in an auxiliary mode according to corresponding diagnosis standards, the test kit has the advantages of being high in sensitivity and strong in specificity, reliability of the diagnosis is far higher than that of diagnosis which is carried out with each single protein landmark, and the test kit has great value and application prospects for diagnosis and treatment of non-small cell lung cancer.

The invention relates to a process of fermenting plant material in a fermentation medium into a fermentation product using a fermenting organism, wherein one or more carbonic anhydrases are present in the fermentation medium.

The nanometer carbonic anhydrse grain for biological catalysis of polymer and its preparation process belongs to the field of chemical modification of enzyme. The nanometer carbonic anhydrse grain with bioactivity is a core-shell structure with core of carbonic anhydrse, shell of crosslinked polymer material and chemical bond connection between the core and the shell, and possesses high catalytic activity, high heat stability and no aggregation. This kind of nanometer carbonic anhydrse grain has wide application foreground in CO2 absorption and separation and artificial lung system, etc. Its preparation process includes introducing C-C double bond radical with enzyme modifier to the surface of carbonic anhydrse and subsequent free radical polymerization with the olefin monomer with C-C double bond as material.

The present invention provides bioactive hollow nano-fibers and hollow microcapsules for efficiently catalyzing methanol synthesis through CO2. According to the present invention, a coaxial co-spinning technology is used to embed three enzymes for catalyzing methanol synthesis through CO2 and comprising formic dehydrogenase, formaldehyde dehydrogenase and ethanol dehydrogenase, and coenzyme NADH into the chamber of polyelectrolyte-doped hollow nano-fibers or hollow microcapsules; in order to achieve coenzyme regeneration, an oxidation reduction enzyme R adopting NAD<+> as coenzyme is embedded into the chamber; in order to accelerate a CO2 hydration reaction, carbonic anhydrase is immobilized on the outer surface of the hollow nano-fibers or hollow microcapsules through a layer-by-layer self-assembly technology; the small molecule NADH is bound on the inner wall of the hollow nano-fibers or hollow microcapsules through the electrostatic interaction between the NADH and the polyelectrolyte in the shell layer so as to achieve the recycling; and the polyelectrolyte-doped hollow nano-fiber or hollow microcapsule CO2 conversion catalyst has advantages of simple preparation, high conversion rate, and high stability, and provides wide application prospects in the field of the conversion of CO2 into methanol.

The invention discloses a protein and amorphous metal organic framework compound and a preparation method thereof. The organic framework compound has a 2 nm-50 nm mesoporous structure. The preparationmethod comprises the following step of enabling a protein, zinc ions and an organic ligand to react in a solvent, wherein the organic ligand is a compound containing an imidazole group; the protein is one or a combination of several kinds of cytochrome C, cytochrome P450, horse radish peroxidase, alcohol dehydrogenase, lipase, acetylcholin esterase, laccase, a green fluorescent protein, glucose dehydrogenase, glucose oxidase, trypsin, bacillus subtilis protease, carbonic anhydrase, aldehyde ketone reductase, amylase, saccharase, superoxide dismutase, urease and catalase. The preparation method of the protein and amorphous metal organic framework compound provided by the invention is simple to operate and mild in condition, the obtained product is high in protein embedding rate and good inprotein stability, and the biologic al activity of the protein is reserved to a greater extent.

The process for obtaining 4-(N-alkylamino)-5,6-dihydro-4H-thien-(2,3-b)-thiopyran-2-sulfonamide-7,7-dioxides (I) wherein R1 is H or C1-5 alkyl, and R2 is C1-5 alkyl, starts from the corresponding 4-(N-alkylamino)-5,6-dihydro-4H-thien-(2,3-b)-thiopyran-7,7-dioxides, and comprises protecting the alkylamine group, introducing a sulfonamide group and eliminating protecting group. Some compounds of formula (I) are inhibitors of the carbonic anhydrase and can be used in the treatment of elevated intraocular pressure