163 results about "Carbonic anhydrase" patented technology

The present disclosure provides drug responsive domains derived from human carbonic anhydrase 2 that can modulate protein stability for human interleukin 15 (IL15) payloads, as well as compositions and methods of use thereof.

The invention discloses a novel method for solidifying a foundation through cooperation of microorganisms and active magnesium oxide, and belongs to the technical field of foundation treatment. In a traditional foundation treatment method, a large amount of ore energy needs to be consumed in the production process of a reinforcing material, harm can be brought to the surrounding environment, and serious noise pollution, air pollution and the like are generated. According to the novel method for solidifying the foundation through cooperation of microorganisms and active magnesium oxide, the carbonation reaction between HCO3 <-> in a NaHCO3 solution and active MgO is accelerated through the catalytic acceleration effect of carbonic anhydrase on the CO2 hydration reaction, so that a large number of carbonates such as magnesium carbonate and basic magnesium carbonate with the good gelling effect are generated, solidified soil particles are cemented, and the effect of solidifying the foundation through cooperation of microorganisms and active magnesium oxide is achieved. And the effect of reinforcing the foundation is achieved.

The invention discloses a Pickering IL emulsion microreactor constructed on the basis of temperature-sensitive double-enzyme Janus spheres and application of the Pickering IL emulsion microreactor to enhanced catalysis of conversion of CO2 into formic acid. A Janus emulsion template method is combined with a photocuring technology to synthesize a nano-scale dual-functional temperature-sensitive Janus sphere, one half of the nano-scale dual-functional temperature-sensitive Janus sphere is a hydrophilic hydrocarbon polymer, the other half of the nano-scale dual-functional temperature-sensitive Janus sphere is a hydrophobic fluorocarbon polymer, and rapid inversion of a Pickering emulsion can be realized by utilizing temperature sensitivity. The method comprises the following steps: preparing a Janus particle hydrophilic / hydrophobic hemisphere, selectively immobilizing formate dehydrogenase FDH and carbonic anhydrase CA on the Janus particle hydrophilic / hydrophobic hemisphere to obtain a temperature-sensitive FDH-Janus-CA composite sphere, introducing a fluorine-functionalized ionic liquid to regulate and control the internal microenvironment of a microreactor, and constructing a Pickering IL emulsion microreactor. The CO2 absorption capacity is increased by 47 times compared with that of a pure water system, the formic acid yield reaches 27.5 mM and is higher than the maximum value 22.8 mM of current enzyme electrocatalysis, the storage and catalysis stability is good, and an efficient and sustainable scheme is provided for CO2 recycling.

The invention belongs to the technical field of fish breeding, and discloses a method for creating alkali-resistant grass carp new germplasm based on a ca12 gene editing technology and application. Carbonic anhydrase ca12 is used as a target gene, a CRISPR / Cas9 gene editing technology is used for carrying out gene editing on wild grass carp, the genotype of the grass carp is identified through sequencing, ca12 gene knockout F0-generation chimera and the corresponding wild type thereof are selected to carry out carbonate alkalinity stress experiments, and based on experimental results, it is known that the ca12 knockout chimera of the grass carp has high alkali resistance, and can be used for preparing the grass carp. The invention provides a novel breeding method and germplasm material for culturing fishes suitable for alkaline water culture, and has important industrial application value.

The invention relates to a carbonic anhydrase IX targeted molecular probe and application thereof, and belongs to the technical field of nuclear medicine. The invention provides carbonic anhydrase IX targeted molecular probes 18F-SAZ and 18F-DAZ, and the two molecular probes have the following advantages: firstly, the specificity is strong, and the molecular probes can be selectively taken in a CAIX positive tumor part so as to better distinguish carbonic anhydrase IX negative and positive tumors; secondly, the compound can quickly reach a carbonic anhydrase IX positive tumor, a better imaging effect can be obtained within 30 minutes, and the compound has better retention property; and thirdly, the in-vivo metabolism is rapid, the biological half-life period is shorter, the in-vivo clearing time is obviously shorter than that of an antibody molecular probe, and the safety is higher. In conclusion, the two molecular probes can dynamically monitor the change of the level of carbonic anhydrase IX in tumor cells in real time through PET imaging, and have great application prospects in diagnosis of positive tumors of carbonic anhydrase IX.

The present invention relates to a method for capturing CO2 using a stabilized enzyme.

The invention discloses a method for quantifying response of two carbon utilization modes of microalgae to weathered carbon sink of magnesium-containing silicate rock, which comprises the following steps: culturing two groups of microalgae under the same culture condition, adding magnesium-containing silicate rock and microalgae into one group, and setting an inhibitor (AZ / DIDS) added with a certain carbon utilization mode as an experimental group; and only magnesium-containing silicate rock and microalgae are added into one group as a blank group without adding an inhibitor (AZ / DIDS) of a certain carbon utilization mode. Setting the treatment group added with AZ (10mM) and DIDS (2mM) as an experimental group, and subtracting the weathering quantity of the magnesium-containing silicate rocks in the treatment group not added with AZ / DIDS and the treatment group added with AZ / DIDS to obtain the weathering quantity delta Ti of the magnesium-containing silicate rocks under the influence of the microalgae anion channel utilization mode, namely delta Ti = Ti-Ti (AZ / DIDS), and rock weathering carbon sink Qi = delta Ti under the influence of the microalgae anion channel utilization mode. Fitting a polynomial function Q = Q (i) to obtain the response function of the microalgae extracellular carbonic anhydrase mode or the microalgae anion channel utilization mode to the weathering carbon sink of the magnesium-containing silicate rock. According to the method, the rock weathering carbon sink quantity under the influence of two carbon utilization modes of the microalgae can be rapidly and accurately obtained. The invention provides the method for quantifying the response of the two carbon utilization modes of the microalgae to the weathered carbon sink of the magnesium-containing silicate rock so as to overcome the defect that the response of the two carbon utilization modes of the microalgae to the weathered carbon sink of the magnesium-containing silicate rock cannot be quantified in the prior art.

The application discloses a kind of solid sealing chromium composite cementitious material and preparation method thereof, solid sealing chromium composite cementitious material includes the following mass percentage of substance: 65wt.%~75wt.% of clinker with heavy metal content, 20wt.%~34wt.% of poly-element auxiliary cementitious material, the rest is gypsum;Total chromium content in clinker with heavy metal content is greater than 150mg / kg;Poly-element auxiliary cementitious material includes the following mass percentage of substance: 30wt.%~50wt.% of microbial mineralization steel slag powder, 20wt.%~40wt.% of metakaolin, the rest is fly ash;Microbial mineralization steel slag powder is prepared by carbonic anhydrase producing bacteria and steel slag powder carbon fixation grinding.The application is based on the synergistic effect between clinker with heavy metal and the overall chemical composition of poly-element composite auxiliary cementitious material, and the pH value of solidified body is reduced by secondary hydration.

The invention belongs to the field of bioengineering, and particularly relates to acid-resistant recombinant escherichia coli, a construction method thereof and application of the acid-resistant recombinant escherichia coli in carbon sequestration synthesis of succinic acid in a low-pH environment. Escherichia coli for producing succinic acid is used as a starting strain, overexpression plasmids of carbonic anhydrase genes from two different sources are constructed, and the overexpression plasmids are introduced into the starting strain to obtain an overexpression strain. The two constructed acid-resistant Escherichia coli show good growth and metabolism capabilities in an acid environment, and the yield of succinic acid can reach 80% or above. Through overexpression of carbonic anhydrase, the thalli can still keep high carbonic anhydrase activity in an acid environment, the capacity of the thalli for fixing CO2 to synthesize succinic acid is improved, reduction of acid and alkali consumption in the fermentation and separation process is facilitated, the production cost of succinic acid is reduced, and remarkable social and economic values are achieved.

Provided herein are vesicular nanocatalysts decorated on their surface with enzymes, such as for industrial use. More particularly, the nanocatalysts include outer membrane vesicles (OMVs) presenting on their surface carbonic anhydrase or a functional fragment or derivative thereof. Uses, methods of manufacture, and compositions of the nanocatalysts are also disclosed herein.

Disclosed herein are compounds that target carbonic anhydrase 9 (CA IX) (such as compounds of structural formula (I) or (II) wherein values for variables are as described herein) and methods for the prevention and / or treatment of certain cancers, including solid tumors, using such compounds. Also disclosed herein are pharmaceutically acceptable salts of such compounds and pharmaceutical compositions comprising such compounds or salts for use in such methods of preventing or treating cancers.

The invention discloses mine water pseudomonas as well as a screening method and application thereof in recharge capacity increase, and belongs to the technical field of mine water recharge engineering. A mine water sample is taken back and then filtered through a filter membrane and a vacuum suction filter; after constant-temperature oscillation, inoculating a liquid LB meat extract peptone culture medium for enrichment culture; carrying out strain separation on the bacterium liquid subjected to enrichment culture by adopting a dilution coating plate method; the method comprises the following steps: carrying out streak culture on a single colony on a solid LB culture medium to obtain a pseudomonas colony, the pseudomonas is Pseudomonas sp. B2, the taxonomic name is Pseudomonas sp. B2, the preservation number is GDMCC No.66726, the preservation date is July 22, 2025, and the preservation unit is Guangdong Province Microbial Culture Collection Center, and the screened pseudomonas capable of producing carbonic anhydrase can effectively promote the dissolution of calcite and dolomite, and can be used for producing carbonic anhydrase. The recharge amount is increased, and the recharge efficiency is improved.

This invention discloses a high-strength microbial cemented backfill material for mining with high efficiency in CO2 sequestration and its preparation method. The material uses a composite carbonic anhydrase-producing microorganism composed of *Bacillus mucilaginosus* and *Bacillus halophilus* as its core, in conjunction with soluble calcium salts, porous hydroxyapatite, industrial solid waste aggregates, and an organic acid-nanoparticle composite inducer, to achieve efficient CO2 mineralization and high-strength cementation under extreme high-salt and medium-high temperature environments in mines. Experiments show that the material achieves a 7-day compressive strength of 16.3–20.2 MPa, a CO2 sequestration capacity of 82.6–101.3 g / kg, and a microbial survival rate exceeding 80%, achieving a synergistic improvement in strength and carbon sequestration efficiency. This material has a simple process, is environmentally friendly, and is suitable for mining engineering such as goaf backfilling and roadway support, providing a new path for the integration of green mining and carbon sequestration.

The application discloses a kind of carbonic anhydrase catalysis CO2 Mineralization enhanced bamboo fiber composite material and preparation method thereof, belong to environmental protection material field.The composite material formula includes by weight parts 15-20 parts of terephthalic acid, 1,4-butanediol 16-25 parts, catalyst 0.2-1 part, bamboo fiber 50-55 parts, calcium chloride 5-8 parts, carbonic anhydrase 0.01-0.03 parts, pH regulator 1-2 parts, antioxidant 0.3-1 part.The preparation process is first used carbonic anhydrase mineralization bamboo fiber, and obtains mineralization bamboo fiber, and PBT is synthesized under the catalysis of catalyst to obtain mineralization bamboo fiber-PBT copolyester composite material.The composite material prepared by the scheme has the characteristics of degradability, low cost, good interfacial compatibility and excellent mechanical properties.

The present invention provides carbonic anhydrase variants. The present invention also provides polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

This invention discloses a method for preparing self-healing concrete based on microbial gene programming, belonging to the field of building materials technology. The method includes: constructing a recombinant engineered strain incorporating a urease gene cluster and a carbonic anhydrase gene; preparing a spore suspension and protecting it with a three-layer microencapsulation system of sodium alginate / chitosan / gelatin; preparing nutrient-releasing microspheres encapsulating calcium lactate and urea in a polylactic acid-glycolic acid copolymer; and mixing cement, standard sand, water, fly ash, microencapsulated spore particles, nutrient-releasing microspheres, nano-silica, and modified bentonite to prepare self-healing concrete. This invention enhances the urease activity of the strain through genetic engineering, protects the spores using a three-layer microencapsulation technology, and combines a nutrient-releasing system and nano-synergists to achieve efficient self-repair of concrete cracks.

The invention discloses a preparation method of composite aerogel, which comprises the following steps: stirring and mixing a silk fibroin solution and a methanol solution of Zn (CH3COO) 2.2 H2O, and dropwise adding a methanol solution of 2-methylimidazole for reaction to form a composite system; then adding polyvinyl alcohol, dissolving at high temperature, reacting at 60 DEG C for 0.5-2 hours, repeatedly freezing and unfreezing at-80 DEG C for 3-5 times, and finally freezing and drying to obtain the composite aerogel material. According to the invention, ZIF-8 is grown on silk fibroin in situ, abundant hydrophilic groups are provided by using a beta-folding structure of the ZIF-8, an active center and a zymoprotein environment of natural carbonic anhydrase are simulated, and proton transport and hydration reaction processes are promoted; meanwhile, a multistage pore channel is constructed by combining micropores of ZIF-8 and mesopores of aerogel, so that the cooperation of CO2 adsorption and catalysis is realized. The three-dimensional aerogel structure is beneficial to high dispersion of active sites and material forming, and has a good industrial application prospect.

The invention relates to a thermophilic bacterium comprising a carbonic anhydrase, wherein the carbonic anhydrase is attached to and / or expressed on the surface of the thermophilic bacterium. The invention also relates to specific variants of a carbonic anhydrase, and it relates to a method of capturing carbon dioxide using the thermophilic bacterium or the carbonic anhydrase variants of the invention.

The present invention discloses a hollow ZIF-8 immobilized bienzyme and its preparation method and application, by embedding formate dehydrogenase and carbonic anhydrase in ZIF-67, then growing a layer of ZIF-8 shell on its surface, dissociating ZIF-67, and the formate dehydrogenase and carbonic anhydrase are fixed in the ZIF-8 shell in a free state, thereby maintaining a high enzyme activity to form a hollow ZIF-8 immobilized bienzyme. Utilizing 2-hydroxy-1,4-naphthoquinone coenzyme regeneration, bioelectrocatalytic CO2 reduction is achieved at the cathode. The preparation process of this method is simple to operate and easy to implement. The hollow structure is used to provide a soft microenvironment for the enzyme. Hollow ZIF-8 and carbonic anhydrase can increase the substrate CO2 adsorption capacity. Using 2-hydroxy-1,4-naphthoquinone regeneration can reduce the consumption of expensive coenzymes, and bioelectrocatalytic CO2 reduction has a higher formic acid yield.

The invention discloses a recombinant bacterium for producing mevalonic acid by taking glucose and acetone as co-substrates as well as a construction method and application of the recombinant bacterium, and belongs to the technical field of genetic engineering. The problems that an existing mevalonic acid biosynthesis mode is low in carbon atom economy, limited in theoretical yield and the like are solved. According to the invention, acetone carboxylase, acetoacetyl coenzyme A synthetase, 3-hydroxy-3-methylglutaryl coenzyme A reductase and hydroxymethyl glutaryl-CoA synthetase are subjected to heterologous expression in escherichia coli with an acetyl coenzyme A acetyltransferase gene atoB and a histidine protein kinase gene atoS knocked out, and self carbonic anhydrase is over-expressed; and a new way for synthesizing MVA by using glucose and acetone as a co-substrate is constructed. According to the method, one molecule of CO2 can be fixed in the biosynthesis of mevalonic acid, the yield of carbon atoms in the synthesis process of mevalonic acid is increased, the problem of low yield of carbon atoms in the synthesis process of mevalonic acid by taking glucose as a single substrate is solved, and a new thought is provided for constructing a carbon neutralization type biological manufacturing technology.

The invention provides a method for solidifying copper tailing waste by magnesium oxychloride cement, which comprises the following steps: dissolving carbonic anhydrase in water, mixing with copper tailings, magnesium oxychloride cement, metakaolin and clay, carrying out microwave treatment and dry mixing on the mixture, then adding water, and continuously stirring uniformly to obtain cement slurry; the curing method comprises the steps that cement slurry is injected into a mold or a mine pit and cured after being vibrated and compacted, a direct-current electric field is continuously applied to the slurry in the curing process, carbon dioxide curing and natural curing are intermittently conducted, and the cement component is obtained after curing is completed. The magnesium oxychloride copper tailing cement prepared by the invention can be used as a road base material or a mine backfill material. The magnesium oxychloride cement is used for curing the copper tailing waste, the prepared cement component is high in strength, excellent in water resistance and low in heavy metal leaching toxicity, the engineering requirements of road base laying or pit backfilling and the like can be fully met, and a new way is opened up for harmless treatment and sustainable utilization of the copper tailings.

The invention provides a carbonic anhydrase bacterium-based waste shale gas well carbon dioxide mineralization and storage method. According to the waste shale gas well carbon dioxide mineralization and storage method based on carbonic anhydrase bacteria, the mineralization and storage efficiency and stability of carbon dioxide in a waste shale gas well are remarkably improved, the carbon dioxide hydration reaction is accelerated through carbonic anhydrase bacteria catalysis, rapid precipitation of carbonate minerals is achieved, and the carbon dioxide yield is increased. Meanwhile, the strain activity is maintained in a complex geological environment, and the sealing cost and leakage risk are reduced.

The application discloses a VEGF / TIE-2 double-targeting polypeptide and application thereof in preparation of an antitumor drug, and relates to the technical field of biological medicine.The VEGF / TIE-2 double-targeting polypeptide (named as RTP) provided by the application is composed of an amino acid sequence capable of target-recognizing VEGF and TIE-2, an amino acid sequence capable of self-assembling to form a beta-sheet nanofiber, an amino acid sequence capable of target-recognizing carbonic anhydrase IX and an enzyme-reactive amino acid sequence capable of being specifically cut by MMP-2.RTP can occur conformational change and self-assemble in tumor cells to form stable water-insoluble nanofibers, and can strongly inhibit tumor neovascularization and metastasis through the VEGF and TIE-2 double-signal pathways and inhibit the progress of the tumor.

The present invention relates to protein ligands for carbonic anhydrase IX (CAIX) as a biomedically relevant target. In particular, highly specific ligands can interact exclusively with an antigen, i.e., CAIX, expressed on the surface of tumor cells, sparing healthy target-expressing organs, thereby enabling efficient in vivo drug delivery applications. Ligands can exhibit particularly low dissociation constants and / or enzyme isoform specificity, making them suitable for targeted delivery of payloads, such as therapeutic and / or diagnostic agents, to sites affected by or at risk of diseases or disorders characterized by CAIX expression.

The present invention discloses a series of novel 4-piperazinamide benzenesulfonamide-1,8-naphthalimide derivatives and their preparation methods. The applicant's experimental results indicate that some of the target compounds of the present invention exhibit good inhibitory activity against CA IX and can be used in drugs that inhibit the activity and / or overexpression of carbonic anhydrase IX. Some of the target compounds also exhibit good anti-tumor activity against various tumor cell lines and are expected to be used in the preparation of anti-tumor drugs.

The invention relates to a preparation method of a heavy-atom-free photosensitizer based on a BODIPY dye, the photosensitizer comprises a thiocarbamate light cage, not only can generate singlet oxygen (1O2) under red light irradiation, but also can release carbonyl sulfide (COS), and generates hydrogen sulfide (H2S) under physiological conditions through carbonic anhydrase catalysis. By reasonably adjusting the molecular structure, the singlet oxygen quantum yield and hydrogen sulfide release efficiency of the photosensitizer are remarkably improved. The method is the first case which is reported at present and can simultaneously activate and release the organic optical functional micromolecules of H2S and 1O2 through a single beam of red light. The strategy effectively solves the problem of dependence on oxygen in type II photodynamic therapy (PDT), and further effectively improves the effect of PDT hypoxic tumors. In in-vitro and in-vivo experiments, hydrogen sulfide gas and photodynamic synergic treatment trigger pyroptosis of cells, promote maturation of dendritic cells and stimulate antitumor immune response. Therefore, the invention provides a synergistic treatment strategy combining gas, photodynamic and immunotherapy, and has a remarkable effect in the aspect of hypoxic tumor treatment. The photosensitizer material disclosed by the invention is simple to prepare and low in cost, has a wide application prospect, and particularly provides a solution with huge potential for cancer treatment in the field of biomedicine.

The present disclosure relates to a radiopharmaceutical targeting carbonic anhydrase IX protein, and in particular to a compound of formula (I), which can effectively target the carbonic anhydrase IX protein, has a fast metabolism rate in the body, high specificity, less uptake by non-target organs, low toxic and side effects on non-target organs, is easy to image, and has high safety, and can be used for diagnosing or treating diseases in which carbonic anhydrase IX protein is overexpressed.

The invention relates to the technical field of microorganism culture and screening, and discloses a culture medium, a preparation method thereof and application of the culture medium in screening mineralized microorganisms, and the culture medium contains peptone, yeast extract powder, sodium chloride, magnesium chloride and calcium chloride. The culture medium takes peptone and yeast extract powder as core components, is not only suitable for screening urealysis type mineralized bacteria, but also provides nutrient substances required by growth of amino acid deamination, carbonic anhydrase catalysis or heterotrophic metabolism driven type mineralized microorganisms, comprehensively screens and prevents potential mineralized bacteria from being omitted. The culture medium can be used for accurately and efficiently screening metal surface mineralizable microorganisms. According to the culture medium, the ecological adaptability of marine strains is optimized, peptone and yeast extract powder supply nutrient substances needed by growth of marine bacteria, sodium chloride, magnesium chloride and calcium chloride are mainly used for simulating the marine salinity environment and adjusting the osmotic pressure and enzyme activity of the bacteria, the matching degree with the environment is high, and the enrichment efficiency is high.

The application belongs to the technical field of biology, and discloses a strain for efficiently fixing carbon dioxide and improving succinic acid conversion rate and a construction method thereof. The application discloses a genetically engineered bacterium, which is obtained by overexpressing carbonic anhydrase genes, phosphoenolpyruvate carboxylase genes and phosphoenolpyruvate carboxykinase genes in a succinic acid-producing strain. ‑ The carbonic anhydrase can enhance the conversion efficiency of CO2 to HCO3 , and the phosphoenolpyruvate carboxylase and the phosphoenolpyruvate carboxykinase can realize efficient fixation of C3 and CO2 into C4 intermediates. Experiments prove that the genetically engineered bacterium can effectively produce succinic acid by using glucose, and has a relatively high sugar acid conversion rate (up to 79.80%).

The invention discloses hydrophilic super-macroporous polystyrene (HGPS (at) MAF-7 (at) FDH) composite microspheres loaded with an inorganic / organic hybrid cascade catalytic system and a preparation method of the hydrophilic super-macroporous polystyrene (HGPS (at) MAF-7 (at) FDH) composite microspheres. The method comprises the following steps: coupling HGPS microspheres with an organic ligand triazole through surface epoxy modification, and reacting the HGPS microspheres with a metal ion solution to obtain a metal ion-loaded microsphere skeleton; then dispersing the framework material in a buffer solution, adding an enzyme solution and an organic ligand triazole, and carrying out a one-pot reaction to obtain the HGPS-coated MAF-7-coated FDH composite microsphere which is used as an inorganic / organic hybrid cascade catalytic system to catalyze CO2 conversion to generate formic acid. According to the method, the defects that a nano material is easy to aggregate and collapse and difficult to recover are effectively overcome, and the material has high specific surface area, excellent mass transfer performance, biocompatibility and mechanical stability. The characteristics of the MAF-7 organic ligand and the controllable porous structure are beneficial to adsorption of CO2 gas, and the MAF-7 not only can be used as a nano-enzyme for replacing carbonic anhydrase to catalyze a CO2 hydration reaction, but also can be used as a carrier for immobilizing formate dehydrogenase. According to the HGPS and MAF-7 and FDH composite microspheres, the complexity of a multi-enzyme cascade reaction is simplified, the stability and robustness of enzymes are improved, a substrate channel is shortened, the overall reaction efficiency is improved, and the HGPS and MAF-7 and FDH composite microspheres have excellent popularization prospects in the fields of CO2 bioconversion and wider cascade reaction system biocatalysis.