222 results about "Carbonic anhydrase" patented technology

The invention relates to the technical field of illicium verum wine production, in particular to fermented illicium verum wine and a preparation process thereof. Comprising the following components in parts by weight: 60-80 parts of water, 5-10 parts of acidified star anise, 0.5-2 parts of a balm extract, 0.1-0.5 part of sulfurous acid, 0.1-0.5 part of activated pectinase, 0.5-2 parts of active dry yeast, 1-3 parts of a thiamine nutritional agent, 10-20 parts of white granulated sugar, 0.5-2 parts of bentonite GB and 0.1-0.5 part of casein. In the design, after the star anise is acidified, the taste of the wine can be improved, the wine is mellower and softer, and the complexity and layering sense of aroma can be increased; carbonic anhydrase mainly acts on generation and decomposition of carbonate ions, so that the risk of acid residue can be reduced, and other beneficial components or flavor substances in illicium verum are not interfered; the melissa officinalis extract is rich in various volatile aromatic compounds, so that the wine body can be endowed with unique elegant fragrance and complex layering sense, and the overall flavor experience is enhanced.

The invention relates to a targeted carbonic anhydrase radiopharmaceutical, in particular to a compound which is a compound as shown in a formula I or a tautomer, a stereoisomer, a hydrate, a solvate, a pharmaceutically acceptable salt or a prodrug of the compound as shown in the formula I, the compounds are capable of inhibiting carbonic anhydrase IX or diagnosing and / or treating one or more tumors, cancers or cells expressing carbonic anhydrase IX. # imgabs0 #

The invention discloses application of a mineralized carbon sequestration strain, the mineralized carbon sequestration strain is classified and named as Halomonas sulfidaeresis. ZN2 #, the mineralized carbon sequestration strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.32033. The invention further discloses a preparation method of the mineralized carbon sequestration strain. The mineralization carbon sequestration strain can metabolize to generate urease and carbonic anhydrase at the same time, the two enzymes are subjected to a dual mineralization reaction in combination with a microorganism-induced calcium carbonate precipitation technology, the crystal form of a mineralization product is improved, the mineralization strength is improved, meanwhile, carbonic anhydrase can absorb carbon dioxide in the environment, high-strength soil reinforcement is achieved, meanwhile, carbon dioxide emission is reduced, and the soil quality is improved. Therefore, the dual effects of biomineralization remediation and improvement and carbon sequestration and emission reduction of the soil are achieved, and sustainable development of a soil bioremediation and improvement technology is achieved.

The invention discloses a mineral cemented filling mortar material for mineralizing and sealing CO2 and a preparation method thereof, and relates to the technical field of low-carbon environment-friendly mortar materials, the raw materials comprise carbonic anhydrase-producing microorganisms, aggregate, a calcium source solution and nano zinc oxide; wherein the calcium source solution is an aqueous solution of calcium chloride, calcium nitrate or calcium acetate, the calcium source solution comprises a premixed calcium source solution and a gradient calcium source solution, the concentration of Ca < 2 + > in the premixed calcium source solution is 0.5 mol / L, the concentration of the gradient calcium source solution is 2 mol / L, and the premixed calcium source solution is used after being premixed with the aggregate; the particle size of the nano zinc oxide is 50-100 nm; according to the present invention, the storage amount of carbon dioxide can be increased so as to achieve the requirements of low carbon and carbon reduction, the reaction rate of CO2 hydration to generate HCO3 <-> / CO3 < 2-> can be significantly increased, the generation of HCO3 <-> / CO3 < 2-> can be accelerated, the CO2 hydration reaction and the subsequent gelling effect can be further accelerated, and the environmental protection and low carbon performance can be improved.

The invention discloses a VEGF / TIE-2 dual-targeting polypeptide and application thereof in preparation of antitumor drugs, and relates to the technical field of biological medicines. The VEGF / TIE-2 dual-targeting polypeptide (named as RTP) provided by the invention is formed by combining an amino acid sequence capable of identifying VEGF and TIE-2 in a targeting manner, an amino acid sequence capable of forming beta-sheet nanofibers through self-assembly, an amino acid sequence capable of identifying carbonic anhydrase IX in a targeting manner and an enzyme reactive amino acid sequence capable of realizing MMP-2 specific shearing. The RTP can be subjected to allosteric and self-assembly in tumor cells to form stable water-insoluble nanofibers, the formation and metastasis of tumor neovascularization are strongly inhibited through VEGF and TIE-2 double signal channels, and the tumor progress is inhibited.

The invention relates to the technical field of building materials, in particular to magnesium oxide carbonized foundation solidified soil and a preparation method and application thereof.Soil is adopted as filler, active magnesium oxide is adopted as a cementing material, and meanwhile microorganisms capable of generating carbonic anhydrase are added, so that the carbon sequestration capacity is improved; and a self-developed reinforcing agent is added to reinforce the solidified soil, so that the carbon dioxide absorption amount is synergistically improved, and the prepared solidified soil is high in mechanical property and carbon sequestration capacity and can be used as a foundation raw material for construction.

The present disclosure provides drug responsive domains derived from human carbonic anhydrase 2 that can modulate protein stability for human interleukin 15 (IL15) payloads, as well as compositions and methods of use thereof.

The invention discloses a novel method for solidifying a foundation through cooperation of microorganisms and active magnesium oxide, and belongs to the technical field of foundation treatment. In a traditional foundation treatment method, a large amount of ore energy needs to be consumed in the production process of a reinforcing material, harm can be brought to the surrounding environment, and serious noise pollution, air pollution and the like are generated. According to the novel method for solidifying the foundation through cooperation of microorganisms and active magnesium oxide, the carbonation reaction between HCO3 <-> in a NaHCO3 solution and active MgO is accelerated through the catalytic acceleration effect of carbonic anhydrase on the CO2 hydration reaction, so that a large number of carbonates such as magnesium carbonate and basic magnesium carbonate with the good gelling effect are generated, solidified soil particles are cemented, and the effect of solidifying the foundation through cooperation of microorganisms and active magnesium oxide is achieved. And the effect of reinforcing the foundation is achieved.

The invention discloses a Pickering IL emulsion microreactor constructed on the basis of temperature-sensitive double-enzyme Janus spheres and application of the Pickering IL emulsion microreactor to enhanced catalysis of conversion of CO2 into formic acid. A Janus emulsion template method is combined with a photocuring technology to synthesize a nano-scale dual-functional temperature-sensitive Janus sphere, one half of the nano-scale dual-functional temperature-sensitive Janus sphere is a hydrophilic hydrocarbon polymer, the other half of the nano-scale dual-functional temperature-sensitive Janus sphere is a hydrophobic fluorocarbon polymer, and rapid inversion of a Pickering emulsion can be realized by utilizing temperature sensitivity. The method comprises the following steps: preparing a Janus particle hydrophilic / hydrophobic hemisphere, selectively immobilizing formate dehydrogenase FDH and carbonic anhydrase CA on the Janus particle hydrophilic / hydrophobic hemisphere to obtain a temperature-sensitive FDH-Janus-CA composite sphere, introducing a fluorine-functionalized ionic liquid to regulate and control the internal microenvironment of a microreactor, and constructing a Pickering IL emulsion microreactor. The CO2 absorption capacity is increased by 47 times compared with that of a pure water system, the formic acid yield reaches 27.5 mM and is higher than the maximum value 22.8 mM of current enzyme electrocatalysis, the storage and catalysis stability is good, and an efficient and sustainable scheme is provided for CO2 recycling.

The invention belongs to the technical field of fish breeding, and discloses a method for creating alkali-resistant grass carp new germplasm based on a ca12 gene editing technology and application. Carbonic anhydrase ca12 is used as a target gene, a CRISPR / Cas9 gene editing technology is used for carrying out gene editing on wild grass carp, the genotype of the grass carp is identified through sequencing, ca12 gene knockout F0-generation chimera and the corresponding wild type thereof are selected to carry out carbonate alkalinity stress experiments, and based on experimental results, it is known that the ca12 knockout chimera of the grass carp has high alkali resistance, and can be used for preparing the grass carp. The invention provides a novel breeding method and germplasm material for culturing fishes suitable for alkaline water culture, and has important industrial application value.

The invention relates to a carbonic anhydrase IX targeted molecular probe and application thereof, and belongs to the technical field of nuclear medicine. The invention provides carbonic anhydrase IX targeted molecular probes 18F-SAZ and 18F-DAZ, and the two molecular probes have the following advantages: firstly, the specificity is strong, and the molecular probes can be selectively taken in a CAIX positive tumor part so as to better distinguish carbonic anhydrase IX negative and positive tumors; secondly, the compound can quickly reach a carbonic anhydrase IX positive tumor, a better imaging effect can be obtained within 30 minutes, and the compound has better retention property; and thirdly, the in-vivo metabolism is rapid, the biological half-life period is shorter, the in-vivo clearing time is obviously shorter than that of an antibody molecular probe, and the safety is higher. In conclusion, the two molecular probes can dynamically monitor the change of the level of carbonic anhydrase IX in tumor cells in real time through PET imaging, and have great application prospects in diagnosis of positive tumors of carbonic anhydrase IX.

The invention relates to a biomass energy utilization and resource utilization technology, and aims to provide a method for improving the carbon sequestration capability of nannochloropsis oculata by domesticating and regulating a key enzyme with high ammonium salt. The method comprises the following steps: carrying out nitrogen salt gradient domestication on nannochloropsis oculata cells, and regulating and controlling the expression quantity of five key enzymes, namely H < + > transport ATP enzyme, glutamine synthetase, asparagine synthetase, carbonic anhydrase and ribulose-1, 5-diphosphate carboxylase / oxygenase, in the nannochloropsis oculata cells; the domesticated and cultured nannochloropsis oculata can independently use coked ammonium sulfate as a nitrogen source required by growth; the domesticated algal strains are subjected to transfer culture, and verification and screening are carried out aiming at the capability of fixing CO2 in flue gas and the prevention of over-acidification of the culture medium. According to the method, the utilization of coked ammonium sulfate by the nannochloropsis oculata can be enhanced without causing over-acidification of the culture medium, the microalgae culture cost can be effectively saved, and the flue gas CO2 immobilization capability of the nannochloropsis oculata is improved; a new thought is provided for large-scale breeding and cultivation by using coked ammonium sulfate and improvement of economic efficiency.

The invention discloses an engineering strain for producing uridine diphosphate, a construction method and a method for synthesizing rebaudioside M, and belongs to the technical field of gene engineering. According to the engineering strain, genes such as a pyrimidine nucleoside operon gene pyrBCAKDFE, a carbonic anhydrase gene cynT and a carbamyl phosphate synthetase gene pyrAA / ABE951 * are integrated on a genome, and genes such as an aspartate carbamyl transaminase gene pyrB, an orotic acid phosphoribose transferase gene pyrE and a phosphoribose pyrophosphate synthase gene prsD128A are deleted. The engineering strain can efficiently synthesize uridine diphosphate, and can act together with a functional protein expression recombinant strain to catalytically synthesize rebaudioside M by taking sucrose and rebaudioside A as substrates.

The invention provides methods and compositions that are useful for treating allergic diseases, bacterial infections, fungal infections, viral infections, mastocytosis, mast cell-mediated inflammation and parasite infections (e.g., helminth infections).

The invention discloses a CAIX targeted degradation agent. The CAIX targeted degradation agent comprises a CAIX target protein ligand, an integrin ligand and a connector, the CAIX target protein ligand and the integrin ligand are connected through the connecting body. As the carbonic anhydrase IX is enriched in the tumor region, the CAIX targeted degradation agent can specifically target the tumor region; the compound can effectively induce degradation of CAIX and inhibit survival of tumor cells under the hypoxia condition, has the functions of targeting a tumor area and degrading carbonic anhydrase IX by utilizing integrin and lysosome, further changes hypoxia and subacid environments of the internal environment of a tumor patient, and plays a role in inhibiting survival of hypoxia tumor cells; the tumor targeting property is high, the toxic and side effects on normal cells are relatively small, and a relatively good effect can be obtained by using a relatively small dosage.

The present invention relates to a method for capturing CO2 using a stabilized enzyme.

The invention discloses a method for quantifying response of two carbon utilization modes of microalgae to weathered carbon sink of magnesium-containing silicate rock, which comprises the following steps: culturing two groups of microalgae under the same culture condition, adding magnesium-containing silicate rock and microalgae into one group, and setting an inhibitor (AZ / DIDS) added with a certain carbon utilization mode as an experimental group; and only magnesium-containing silicate rock and microalgae are added into one group as a blank group without adding an inhibitor (AZ / DIDS) of a certain carbon utilization mode. Setting the treatment group added with AZ (10mM) and DIDS (2mM) as an experimental group, and subtracting the weathering quantity of the magnesium-containing silicate rocks in the treatment group not added with AZ / DIDS and the treatment group added with AZ / DIDS to obtain the weathering quantity delta Ti of the magnesium-containing silicate rocks under the influence of the microalgae anion channel utilization mode, namely delta Ti = Ti-Ti (AZ / DIDS), and rock weathering carbon sink Qi = delta Ti under the influence of the microalgae anion channel utilization mode. Fitting a polynomial function Q = Q (i) to obtain the response function of the microalgae extracellular carbonic anhydrase mode or the microalgae anion channel utilization mode to the weathering carbon sink of the magnesium-containing silicate rock. According to the method, the rock weathering carbon sink quantity under the influence of two carbon utilization modes of the microalgae can be rapidly and accurately obtained. The invention provides the method for quantifying the response of the two carbon utilization modes of the microalgae to the weathered carbon sink of the magnesium-containing silicate rock so as to overcome the defect that the response of the two carbon utilization modes of the microalgae to the weathered carbon sink of the magnesium-containing silicate rock cannot be quantified in the prior art.

The application discloses a kind of solid sealing chromium composite cementitious material and preparation method thereof, solid sealing chromium composite cementitious material includes the following mass percentage of substance: 65wt.%~75wt.% of clinker with heavy metal content, 20wt.%~34wt.% of poly-element auxiliary cementitious material, the rest is gypsum;Total chromium content in clinker with heavy metal content is greater than 150mg / kg;Poly-element auxiliary cementitious material includes the following mass percentage of substance: 30wt.%~50wt.% of microbial mineralization steel slag powder, 20wt.%~40wt.% of metakaolin, the rest is fly ash;Microbial mineralization steel slag powder is prepared by carbonic anhydrase producing bacteria and steel slag powder carbon fixation grinding.The application is based on the synergistic effect between clinker with heavy metal and the overall chemical composition of poly-element composite auxiliary cementitious material, and the pH value of solidified body is reduced by secondary hydration.

The invention belongs to the field of bioengineering, and particularly relates to acid-resistant recombinant escherichia coli, a construction method thereof and application of the acid-resistant recombinant escherichia coli in carbon sequestration synthesis of succinic acid in a low-pH environment. Escherichia coli for producing succinic acid is used as a starting strain, overexpression plasmids of carbonic anhydrase genes from two different sources are constructed, and the overexpression plasmids are introduced into the starting strain to obtain an overexpression strain. The two constructed acid-resistant Escherichia coli show good growth and metabolism capabilities in an acid environment, and the yield of succinic acid can reach 80% or above. Through overexpression of carbonic anhydrase, the thalli can still keep high carbonic anhydrase activity in an acid environment, the capacity of the thalli for fixing CO2 to synthesize succinic acid is improved, reduction of acid and alkali consumption in the fermentation and separation process is facilitated, the production cost of succinic acid is reduced, and remarkable social and economic values are achieved.

Provided herein are vesicular nanocatalysts decorated on their surface with enzymes, such as for industrial use. More particularly, the nanocatalysts include outer membrane vesicles (OMVs) presenting on their surface carbonic anhydrase or a functional fragment or derivative thereof. Uses, methods of manufacture, and compositions of the nanocatalysts are also disclosed herein.

Disclosed herein are compounds that target carbonic anhydrase 9 (CA IX) (such as compounds of structural formula (I) or (II) wherein values for variables are as described herein) and methods for the prevention and / or treatment of certain cancers, including solid tumors, using such compounds. Also disclosed herein are pharmaceutically acceptable salts of such compounds and pharmaceutical compositions comprising such compounds or salts for use in such methods of preventing or treating cancers.

Described herein are systems and methods for supplying an algae cultivation fluid with carbon by incorporating microorganisms that express carbonic anhydrase. The systems and methods further include capturing carbon dioxide directly from the atmosphere for absorption by the carbonic anhydrase.

The invention discloses mine water pseudomonas as well as a screening method and application thereof in recharge capacity increase, and belongs to the technical field of mine water recharge engineering. A mine water sample is taken back and then filtered through a filter membrane and a vacuum suction filter; after constant-temperature oscillation, inoculating a liquid LB meat extract peptone culture medium for enrichment culture; carrying out strain separation on the bacterium liquid subjected to enrichment culture by adopting a dilution coating plate method; the method comprises the following steps: carrying out streak culture on a single colony on a solid LB culture medium to obtain a pseudomonas colony, the pseudomonas is Pseudomonas sp. B2, the taxonomic name is Pseudomonas sp. B2, the preservation number is GDMCC No.66726, the preservation date is July 22, 2025, and the preservation unit is Guangdong Province Microbial Culture Collection Center, and the screened pseudomonas capable of producing carbonic anhydrase can effectively promote the dissolution of calcite and dolomite, and can be used for producing carbonic anhydrase. The recharge amount is increased, and the recharge efficiency is improved.

This invention discloses a high-strength microbial cemented backfill material for mining with high efficiency in CO2 sequestration and its preparation method. The material uses a composite carbonic anhydrase-producing microorganism composed of *Bacillus mucilaginosus* and *Bacillus halophilus* as its core, in conjunction with soluble calcium salts, porous hydroxyapatite, industrial solid waste aggregates, and an organic acid-nanoparticle composite inducer, to achieve efficient CO2 mineralization and high-strength cementation under extreme high-salt and medium-high temperature environments in mines. Experiments show that the material achieves a 7-day compressive strength of 16.3–20.2 MPa, a CO2 sequestration capacity of 82.6–101.3 g / kg, and a microbial survival rate exceeding 80%, achieving a synergistic improvement in strength and carbon sequestration efficiency. This material has a simple process, is environmentally friendly, and is suitable for mining engineering such as goaf backfilling and roadway support, providing a new path for the integration of green mining and carbon sequestration.

The application discloses a kind of carbonic anhydrase catalysis CO2 Mineralization enhanced bamboo fiber composite material and preparation method thereof, belong to environmental protection material field.The composite material formula includes by weight parts 15-20 parts of terephthalic acid, 1,4-butanediol 16-25 parts, catalyst 0.2-1 part, bamboo fiber 50-55 parts, calcium chloride 5-8 parts, carbonic anhydrase 0.01-0.03 parts, pH regulator 1-2 parts, antioxidant 0.3-1 part.The preparation process is first used carbonic anhydrase mineralization bamboo fiber, and obtains mineralization bamboo fiber, and PBT is synthesized under the catalysis of catalyst to obtain mineralization bamboo fiber-PBT copolyester composite material.The composite material prepared by the scheme has the characteristics of degradability, low cost, good interfacial compatibility and excellent mechanical properties.

The present invention provides carbonic anhydrase variants. The present invention also provides polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

This invention discloses a method for preparing self-healing concrete based on microbial gene programming, belonging to the field of building materials technology. The method includes: constructing a recombinant engineered strain incorporating a urease gene cluster and a carbonic anhydrase gene; preparing a spore suspension and protecting it with a three-layer microencapsulation system of sodium alginate / chitosan / gelatin; preparing nutrient-releasing microspheres encapsulating calcium lactate and urea in a polylactic acid-glycolic acid copolymer; and mixing cement, standard sand, water, fly ash, microencapsulated spore particles, nutrient-releasing microspheres, nano-silica, and modified bentonite to prepare self-healing concrete. This invention enhances the urease activity of the strain through genetic engineering, protects the spores using a three-layer microencapsulation technology, and combines a nutrient-releasing system and nano-synergists to achieve efficient self-repair of concrete cracks.

The invention discloses a preparation method of composite aerogel, which comprises the following steps: stirring and mixing a silk fibroin solution and a methanol solution of Zn (CH3COO) 2.2 H2O, and dropwise adding a methanol solution of 2-methylimidazole for reaction to form a composite system; then adding polyvinyl alcohol, dissolving at high temperature, reacting at 60 DEG C for 0.5-2 hours, repeatedly freezing and unfreezing at-80 DEG C for 3-5 times, and finally freezing and drying to obtain the composite aerogel material. According to the invention, ZIF-8 is grown on silk fibroin in situ, abundant hydrophilic groups are provided by using a beta-folding structure of the ZIF-8, an active center and a zymoprotein environment of natural carbonic anhydrase are simulated, and proton transport and hydration reaction processes are promoted; meanwhile, a multistage pore channel is constructed by combining micropores of ZIF-8 and mesopores of aerogel, so that the cooperation of CO2 adsorption and catalysis is realized. The three-dimensional aerogel structure is beneficial to high dispersion of active sites and material forming, and has a good industrial application prospect.

The invention relates to a thermophilic bacterium comprising a carbonic anhydrase, wherein the carbonic anhydrase is attached to and / or expressed on the surface of the thermophilic bacterium. The invention also relates to specific variants of a carbonic anhydrase, and it relates to a method of capturing carbon dioxide using the thermophilic bacterium or the carbonic anhydrase variants of the invention.