Method for repairing crack of cement-based material

A technology of cement-based materials and cementitious materials, which is applied in the field of interdisciplinary science, can solve the problems that organic matter is not environmentally friendly and the repair effect is not ideal, and achieve fast carbonate deposition rate, slow down the greenhouse effect, and polycarbonate The effect of sedimentation

Active Publication Date: 2013-10-09
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although traditional repair methods such as inorganic repair and organic repair are widely used, inorganic repair such as grouting repair is simple but the repair effect is not ideal. Although organic repair can achieve good results, the durability of organic matter restricts its application. , and most organic compounds are not environmentally friendly

Method used

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  • Method for repairing crack of cement-based material
  • Method for repairing crack of cement-based material
  • Method for repairing crack of cement-based material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] (1) Weigh 12g of sucrose, Na 2 HPO 4 12H 2 O 3g, MgSO 4 0.6g, CaCO 3 1.5g, KCl 0.2g, (NH 4 ) 2 SO 4 Dissolve 0.6g and 0.4g of yeast extract in 1000mL deionized water, prepare the required medium solution, adjust the pH to 7, sterilize at 125°C for 25 minutes, take it out and wait for cooling, inoculate Bacillus colloidus to cooling culture In the basal solution, shake culture at 30°C, the shaking frequency is 170 r / min, and the culture time is 24h;

[0045] (2) Centrifuge the cultured bacterial solution at high speed for 10 minutes at a speed of 8000 rpm and a temperature of 4°C to remove nutrients from the upper layer of the culture medium and add 100 mL of deionized water to make a concentrated bacterial solution;

[0046] (3) Soak 200g of ceramsite in 100mL of Bacillus mucilaginous concentrated bacterial solution for 24h and set aside for use. The water-to-cement ratio of the cement-based material is W / C=0.36, the amount of cementitious material is 1300g, a...

Embodiment 2

[0050] (1) Weigh 9.6g of sucrose, Na 2 HPO 4 12H 2 O 2.4g, MgSO 4 0.48g, CaCO 3 1.2g, KCl 0.16g, (NH 4 ) 2 SO 4 Dissolve 0.48g and 0.32g of yeast extract in 800mL of deionized water, prepare the required medium solution, adjust the pH to 7, sterilize at 125°C for 25 minutes, take it out and wait for cooling, inoculate Bacillus colloidus to cooling culture In the basal solution, shake culture at 30°C, the shaking frequency is 170 r / min, and the culture time is 24h;

[0051] (2) Centrifuge the cultured bacterial solution at high speed for 10 minutes at a speed of 8,000 rpm and a temperature of 4°C to remove the nutrients in the upper medium, and add 80 mL of deionized water to make a concentrated bacterial solution;

[0052] (3) Soak 150g of ceramsite in 80mL of Bacillus mucilaginous concentrated bacterial solution for 24h and set aside. The water-to-cement ratio of the cement-based material is W / C=0.36, the amount of cementitious material is 1300g, and 40g of tetrahyd...

Embodiment 3

[0056] (1) Weigh 8g of sucrose, Na 2 HPO 4 12H 2 O 2g, MgSO 4 0.4g, CaCO 3 0.5g, KCl 0.1g, (NH 4 ) 2 SO 4 Dissolve 0.4g and 0.2g of yeast extract in 500mL deionized water, prepare the required medium solution, adjust the pH to 7, sterilize at 125°C for 25 minutes, take it out and wait for cooling, inoculate Bacillus colloidus to cooling culture In the basal solution, shake culture at 30°C, the shaking frequency is 170 r / min, and the culture time is 24h;

[0057] (2) Centrifuge the cultured bacterial solution at high speed for 10 minutes at a speed of 8,000 rpm and a temperature of 4°C to remove the nutrients in the upper layer of the culture medium, and add 50 mL of deionized water to make a concentrated bacterial solution;

[0058] (3) Soak 100g of ceramsite in 50mL of Bacillus mucilaginous concentrated bacterial solution for 24h and set aside. The water-to-cement ratio of the cement-based material is W / C=0.36, the amount of cementitious material is 1300g, and 65g p...

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Abstract

The invention discloses a method for repairing a crack of a cement-based material. According to the method, carbonic anhydrase microorganisms are adopted for generating carbonic anhydrase used for catching CO2, CO2 is promoted to be converted into CO3<2->, and the deposition of calcium carbonate within a surface area of the crack is accelerated; the method has the advantage that the repair speed is faster than that of other methods for repairing the crack through the microorganisms. The method comprises the following steps of: inoculating bacillus mucilaginosus to a culture medium; culturing; preparing bacillus mucilaginosus concentrated bacteria liquid; immobilizing bacteria on carriers; uniformly burying calcium sources into the cement-based material during forming the cement-based material; concentrating the carriers with the bacteria immobilized into a range of 5mm under a surface area of a test part; manufacturing the crack after a part to be tested is cured; transferring into a thermostatic waterbath; continuously charging air for maintaining and repairing. The repair test result shows that the osmotic coefficient is greatly reduced after repairing for 5 days, and the crack is repaired completely after continuously repairing for 30 days.

Description

[0001] technical field [0002] The invention belongs to the interdisciplinary science and technology in the fields of microbiology and building materials, and relates to a novel microbial crack repair technology. [0003] Background technique [0004] Cement concrete is currently the most widely used building material. Among the many factors that cause the durability of cement concrete to decline, the cracking problem can be called the "cancer" of cement concrete and has always troubled many cement concrete researchers. Although traditional repair methods such as inorganic repair and organic repair are widely used, inorganic repair such as grouting repair is simple but the repair effect is not ideal. Although organic repair can achieve good results, the durability of organic matter restricts its application. , and most organic compounds are not environmentally friendly. [0005] Microbial self-healing concrete is a new type of intelligent material with self-perception and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C04B14/02
CPCC12N1/20C04B20/1003C04B2111/00663C04B2111/72C04B18/027C04B2103/0001C04B14/12
Inventor 钱春香任立夫荣辉李瑞阳
Owner SOUTHEAST UNIV
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