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Method for repairing crack of cement-based material

A technology for cement-based materials and cementitious materials, which is applied in the field of microbiology and building materials, can solve the problems that organic matter does not have environmental friendly characteristics and the restoration effect is not ideal, achieves fast carbonate deposition rate, slows down the greenhouse effect, The effect of fast calcium ion deposition rate

Active Publication Date: 2014-09-03
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although traditional repair methods such as inorganic repair and organic repair are widely used, inorganic repair such as grouting repair is simple but the repair effect is not ideal. Although organic repair can achieve good results, the durability of organic matter restricts its application. , and most organic compounds are not environmentally friendly

Method used

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  • Method for repairing crack of cement-based material
  • Method for repairing crack of cement-based material
  • Method for repairing crack of cement-based material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Weigh 12g of sucrose and Na 2 HPO 4 ·12H 2 O 3g, MgSO 4 0.6g, CaCO 3 1.5g, KCl 0.2g, (NH 4 ) 2 SO 4 0.6g and 0.4g of yeast extract are dissolved in 1000mL deionized water, prepared into the required medium solution, adjusted to pH 7, sterilized at 125°C for 25 minutes and then taken out to be cooled, inoculated with Bacillus mucilaginosus to cool culture In the base solution, shake culture at 30℃, the shaking frequency is 170 r / min, and the culture time is 24h;

[0039] (2) Centrifuge the cultured bacterial solution at high speed for 10 minutes at a speed of 8000 rpm and a temperature of 4°C to remove nutrients in the upper layer of the medium, and add 100 mL of deionized water to make a concentrated bacterial solution;

[0040] (3) Take 200g ceramsite and soak it in 100mL Bacillus mucilaginous concentrated bacteria solution for 24h, and set aside for 24h. The cement-based material water-binder ratio W / C=0.36, the amount of cementing material is 1300g, and 20g is weighe...

Embodiment 2

[0044] (1) Weigh 9.6g of sucrose and Na 2 HPO 4 ·12H 2 O 2.4g, MgSO 4 0.48g, CaCO 3 1.2g, KCl 0.16g, (NH 4 ) 2 SO 4 0.48g and 0.32g of yeast extract are dissolved in 800mL deionized water, prepared into the required medium solution, adjusted to pH 7, sterilized at 125°C for 25 minutes, then taken out to be cooled, inoculated with Bacillus mucilaginosus to cool culture In the base solution, shake culture at 30℃, the shaking frequency is 170 r / min, and the culture time is 24h;

[0045] (2) Centrifuge the cultured bacterial solution at high speed for 10 minutes at a centrifuge speed of 8000 rpm and a temperature of 4°C to remove nutrients in the upper layer of culture medium, and add 80 mL of deionized water to make a concentrated bacterial solution;

[0046] (3) Take 150g of ceramsite and soak it in 80mL Bacillus mucilaginous concentrated bacteria solution for 24h, and set aside for 24h. The water-binder ratio of the cement-based material is W / C=0.36, the amount of cementing materi...

Embodiment 3

[0050] (1) Weigh 8g of sucrose and Na 2 HPO 4 ·12H 2 O 2g, MgSO 4 0.4g, CaCO 3 0.5g, KCl 0.1g, (NH 4 ) 2 SO 4 0.4g, 0.2g of yeast extract is dissolved in 500mL deionized water, prepared into the required medium solution, adjusted to pH 7, sterilized at 125°C for 25 minutes, then taken out to be cooled, inoculated with Bacillus mucilaginosus to cool culture In the base solution, shake culture at 30℃, the shaking frequency is 170 r / min, and the culture time is 24h;

[0051] (2) Centrifuge the cultured bacterial solution at high speed for 10 minutes at a speed of 8000 rpm and a temperature of 4°C to remove nutrients in the upper layer of culture medium and add 50 mL of deionized water to make a concentrated bacterial solution;

[0052] (3) Take 100g of ceramsite and soak it in 50mL of Bacillus mucilaginous concentrated bacteria solution for 24h, and set aside W / C=0.36 for forming cement-based material, the amount of cementing material is 1300g, and weigh 65g pentahydrate Calcium la...

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Abstract

The invention discloses a method for repairing cracks in cement-based materials, which uses carbonic anhydrase microorganisms to produce carbonic anhydrase to capture CO2, promotes the conversion of CO2 into CO32-, accelerates the deposition of calcium carbonate on the surface of cracks, and repairs cracks compared with other microorganisms. Has the advantage of faster repair speed. The steps are as follows: inoculate Bacillus colloidus into the culture medium and prepare concentrated Bacillus colloidus liquid, immobilize the bacteria on the carrier, embed the calcium source evenly inside the cement-based material when molding the cement-based material, The carrier loaded with bacteria is concentrated within 5mm below the surface area of ​​the specimen. After the specimen is cured, cracks are artificially made, placed in a constant temperature water bath, and continuously ventilated with air for maintenance and repair. The repair test results show that the permeability coefficient is greatly reduced after 5 days of repair, and the cracks are completely repaired after 30 days of continuous repair.

Description

technical field [0001] The invention belongs to the interdisciplinary science and technology in the fields of microbiology and building materials, and relates to a novel microbial crack repair technology. Background technique [0002] Cement concrete is currently the most widely used building material. Among the many factors that cause the durability of cement concrete to decline, the cracking problem can be called the "cancer" of cement concrete and has always troubled many cement concrete researchers. Although traditional repair methods such as inorganic repair and organic repair are widely used, inorganic repair such as grouting repair is simple but the repair effect is not ideal. Although organic repair can achieve good results, the durability of organic matter restricts its application. , and most organic compounds are not environmentally friendly. [0003] Microbial self-healing concrete is a new type of intelligent material with self-perception and self-healing. The ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C04B14/02
CPCC04B28/00C12N1/20C04B20/1003C04B2111/00663C04B2111/72
Inventor 钱春香任立夫荣辉李瑞阳
Owner SOUTHEAST UNIV
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