1720 results about "Yeast" patented technology

The invention relates to a preparation method of a camellia fermentation product, which is characterized in that camellia is fermented by adopting a mode of firstly performing enzymolysis and then performing fermentation, in the fermentation step, a composite yeast strain fermentation mode is adopted, the camellia is fermented through the synergistic effect of two yeasts, and the obtained camellia fermentation product is rich in various active substances. Therefore, the fermented product has good comprehensive effects of repairing, relieving and controlling oil.

The invention discloses a recombinant humanized XVII type collagen as well as an expression vector and a genetically engineered bacterium thereof. Four functional fragments with high activity are obtained through screening, one of the fragments is optimized and then combined according to a certain sequence to obtain recombinant humanized XVII type collagen JYC1703, or one of the fragments is optimized and then combined according to a certain sequence, and GPP sequences are fused at an amino terminal and a carboxyl terminal respectively to obtain recombinant humanized XVII type collagen JYC1701 and JYC1702, and the recombinant humanized XVII type collagen JYC1701 and JYC1702 are combined according to a certain sequence to obtain the recombinant humanized XVII type collagen JYC1701 and JYC1702. After codon optimization, amplifying and cloning into an expression vector, transforming into pichia pastoris competent cells after enzyme digestion linearization, and screening high-copy recombinants through G418 resistance gradient, so as to obtain pichia pastoris genetically engineered bacteria for expressing recombinant humanized XVII type collagen. The recombinant humanized XVII type collagen JYC1701, JYC1702 and JYC1703 disclosed by the invention show excellent functions of promoting cell adhesion, cell proliferation and cell migration, and are suitable for preparing products such as skin care products or medical instruments and the like.

The present invention provides a Yarrowia lipolytica Y1590 and its application. The Yarrowia lipolytica Y1590 is classified and named as Yarrowia lipolytica, and was deposited in the General Microbiological Center of China Microbiological Culture Collection Administration on June 4, 2024, with a deposit address in Beijing, China, and a deposit number of CGMCC No. 30855. It can solve the problem of slow growth of Yarrowia lipolytica in the prior art and is applicable to the field of microbial technology.

The invention discloses a method for reducing saline-alkali water into modern seawater suitable for culturing various seafoods by using microorganisms, a special fish fertilizer for the method and application of a preparation method of the special fish fertilizer, and the method comprises the following specific steps: 1, pretreating the saline-alkali water: pumping saline-alkali with the salinity of 15-30% o into a culture pond, compressing, mixing and precipitating until the pH value is 7.3-7.5; 2, performing microbial culture: performing multi-stage enrichment on halobacterium violaceum and selecting dominant colonies; the method comprises the following steps: mixing underground saline-alkali water with the salinity of 10-20% o, saline-alkali water of a salt lake with the salinity of 180-200% o and high saline-alkali soil containing part of organic matters, cooking and sealing at the temperature of 200-210 DEG C, inoculating selected mixed halobacterium purple, pumping air, culturing with sunlight, and propagating for later use, wherein the pH value is 8.3-8.5; 3, reduction reaction: carrying out reduction reaction in a static water environment, when the water body is red brown or dark brown, inoculating a heterotrophic photosynthetic bacteria liquid and an autotrophic photosynthetic bacteria liquid with concentration, inoculating yeast powder, carrying out common reaction, then inoculating a special aquaculture fish fertilizer preparation, carrying out oxygenation reaction, and adjusting the pH value to 8.5-8.8; real seafood can be cultured in saline-alkali water in deserts and gobi deserts, zero pollution and zero emission can be achieved, the cost of development, implementation and popularization is low, and the cost of 10,000 cubic meters of water is only 675 yuan.

The present application discloses a recombinant bacterium for producing D-psicose by fermentation and a preparation method thereof, and belongs to the field of food chemical industry. The technical problem to be solved by the present application is: how to obtain a recombinant bacterium that uses methanol as a raw material for bacterial growth and glucose as a raw material to synthesize D-psicose. By subjecting the recipient bacteria to a purposeful transformation, the microbial strain is provided with the ability to accumulate microbial biomass using methanol and synthesize D-psicose using D-glucose, while preventing the use of D-glucose for microbial growth so that D-glucose is completely converted into D-psicose, thereby improving substrate utilization efficiency. The recombinant polymorphic Hansen yeast provided in the present application can realize the use of methanol and other compounds as raw materials for microbial growth, and after the microbial growth reaches a certain biomass, D-psicose is synthesized using D-glucose as a raw material. And then establish a corresponding technical route for synthesizing D-psicose.

The present disclosure provides a recombinant yeast host cell having a plurality of native and / or heterologous enzymes that function in an engineered metabolic pathway to convert fructose-6-phosphate to acetyl-coenzyme A. The recombinant yeast host cell can be used, for example, in a process for making acetyl-coenzyme A, acetone and / or isopropanol. The plurality of native and / or heterologous enzymes is activated, upregulated, or overexpressed. The plurality of native and / or heterologous enzymes comprises a phosphoketolase; and optionally an acetate kinase; and / or a phosphotransacetylase. The recombinant yeast host cell comprises at least one of: at least two copies of a heterologous nucleic acid molecule encoding the phosphoketolase; a native and / or heterologous enzyme that functions in an engineered non-oxidative pentose phosphate pathway, wherein the native and / or heterologous enzyme is activated, upregulated, or overexpressed; and / or a native and / or heterologous protein that functions in an engineered metabolic pathway to convert pantothenate into acetyl-coenzyme A, wherein the native and / or heterologous protein is activated, upregulated or overexpressed and comprises at least one of: FEN2 or CAB1.

The invention relates to a manufacturing method for delicious pork slices. To overcome the influence of too much intake of pork products on a human body, the manufacturing method for the delicious pork slices is provided. The manufacturing method comprises the steps of cutting the pork into the pork slices with the thickness of 2-4 mm; immersing the pork slices in an immersion liquid for 10-30 minutes; heating the immersion liquid with the pork slices and cooking for 0.5-1 h; fishing the pork slices out; draining the liquid; packaging and sterilizing. The manufacturing method is characterized in that, based on 100 parts by weight of the pork slices, the immersion liquid comprises, by weight, 1-5 parts of red yeast, 1-10 parts of rice wine, 20-30 parts of soy sauce, 1-5 parts of dried ginger, 10-20 parts of white sugar, 1-5 parts of spices, 1-5 parts of sodium bicarbonate, 15-30 parts of seaweed and 50-60 parts of water. The pork slices processed by the method can effectively prevent damp and hot proliferation in a stomach after being taken, and gives health to a human body while guaranteeing nutrition of the human body.

The invention discloses lactobacillus plantarum for degrading low-molecular aldehyde substances, a composition thereof and application of the lactobacillus plantarum in duck liver fishy smell degradation, and is characterized in that the lactobacillus plantarum is an LPC01 strain, the preservation number is CGMCC No.32002, the addition amount of a lactobacillus plantarum suspension in duck liver paste is 0.5-1.5 vt%, and the addition amount of the lactobacillus plantarum suspension in the duck liver paste is 0.5-1.5 vt%. The invention further provides an application of the lactobacillus plantarum composition composed of the lactobacillus plantarum suspension and the saccharomycetes suspension in equal volume in duck liver fishy smell degradation, the saccharomycetes suspension is inoculated into the duck liver paste for fermentation, then the lactobacillus plantarum suspension is inoculated for fermentation, and the lactobacillus plantarum composition has the advantages that in the duck liver fermentation process, the total aldehyde degradation rate of the strain is 63.8%; and under the co-culture condition with saccharomycetes, the degradation rate is increased to 97.8%, and a remarkable deodorization effect is shown.

The invention belongs to the technical field of white spirit brewing, and particularly relates to bacillus velezensis BL-LM and application thereof in preparation of special-flavor white spirit yeast. The bacterial strain is classified and named as Bacillus velezensis, and is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.30130. The invention further discloses a preparation method of the bacterial strain. When the bacillus velezensis BL-LM is added into starter propagation raw materials, in the starter propagation process, the top fire temperature of a starter blank is high, the temperature rising time is long, the liquefying power and saccharifying power activity of the starter blank is higher, the capacity of producing white spirit characteristic flavor substances such as pyrazines, guaiacol and 4-vinyl guaiacol is higher, and the content of organic acid is high. The bacillus velezensis BL-LM disclosed by the invention has a relatively good promotion effect on physicochemical property optimization and flavor substance generation of the specially-fragrant yeast, and has a very obvious effect in the aspect of modifying the quality of the specially-fragrant yeast.

The preparation method comprises the following steps: firstly, analyzing the amino acid sequence of an alpha 1 chain of the human XVII type collagen, and selecting partial amino acid fragments which are not easily degraded by endogenous or exogenous protease in non-transmembrane regions of different functional domains of the protein; the recombinant collagen gene fragment (Col17a) n is obtained by repeatedly splicing the recombinant collagen gene fragment (Col17a) n; then introducing homologous promoters from different methylotrophic yeasts to the 5'end of a recombinant collagen gene segment, optimally synthesizing selected (Col17a) n and promoter segments, obtaining a connecting segment by utilizing PCR cloning, connecting the collagen segment (Col17a) n, the promoter segments and a pPIC9K carrier skeleton segment by virtue of a Gibson Assessment seamless connection technology, and obtaining the recombinant collagen gene. And constructing to obtain the pichia pastoris expression plasmid pPIC9K-(Col17a) n of the recombinant human source XVII type collagen.

The invention relates to a preparation method of a calendula fermentation product. The preparation method comprises the step of fermenting calendula with saccharomycetes and lactobacillus in sequence. According to the invention, the calendula is subjected to secondary fermentation by adopting a method of firstly fermenting by using the saccharomycetes and then fermenting by using the lactobacillus, and the calendula is fermented through the synergistic effect of the two bacteria, so that the obtained calendula fermented product has the comprehensive effects of moisturizing, repairing and relieving.

The invention relates to the technical field of animal feeds, and provides an animal feed and a preparation method and optimization method thereof, and the optimization method of the animal feed comprises the following steps: determining candidate microorganisms and nutrition raw material formulas, establishing an optimization model, and solving to obtain an optimal microorganism formula and an optimal nutrition raw material formula; the animal feed comprises microorganisms such as lactic acid bacteria, saccharomycetes and bacillus and nutritional raw materials such as corn and soybean meal. By adopting a multilayer microcapsule technology, the survival rate of microorganisms in an acid environment is improved, the digestion and absorption efficiency is improved, and the nutritional condition and the milk production performance are improved; the invention further provides a corresponding preparation method, and the feed has the remarkable slow release characteristic and the effect of improving the feed utilization rate.

The invention relates to the technical field of gene engineering, in particular to a soybean disease-resistant RING-H2 type zinc finger protein gene GmRHF1 and application thereof.According to the technology, the GmRHF1 genes are cloned in soybean varieties Xu bean 14 and Shangshan black beans respectively, and then the expression quantity difference of the genes in different tissues and the expression quantity change trend of the genes under SMV treatment are analyzed through fluorescent quantitative PCR; meanwhile, the anti-SMV function of the GmRHF1 is verified in effective plant silencing and transgenic overexpression by adopting a virus-induced gene transient silencing technology and a soybean stable genetic transformation experiment. Finally, the interaction protein of the GmRHF1 is screened and verified through yeast cDNA library screening, yeast point verification and luciferase complementary test screening, and the biological significance of interaction of the GmRHF1 and the GmClpP6 on soybean SMV resistance is defined. In conclusion, the result of the invention can provide an effective gene resource for cultivating a new variety (line) of soybean with high SMV resistance.

The invention discloses a craft IPA beer flora optimization method based on an improved bat algorithm. The method comprises the following steps: S1, constructing an initial state space of yeast flora proportion dynamic optimization; s2, standardized real-time process data are formed; s3, fusing the standardized real-time process data with the initial state space to obtain an updated comprehensive state vector, and dividing the whole fermentation process into a sugar collection stage, a main fermentation stage and an after-ripening stage according to a time sequence; s4, obtaining a staged entropy weight vector; s5, forming a multi-objective optimization problem; s6, obtaining an optimal flora proportion parameter set and an optimal fermentation regulation parameter set; and S7, transmitting the optimal flora proportion parameter set and the optimal fermentation regulation and control parameter set to a microfluidic bacterium throwing device, a material supplementing control unit and a frequency conversion temperature control unit to realize the optimization of the crafted IPA beer flora. According to the method, an optimization path can be effectively guided to be close to a flavor synergistic expression area, and the spatial consistency of yeast metabolism and aroma generation is greatly improved.

The invention discloses a cattle feed additive. The cattle feed additive consists of the following raw materials in parts by weight: 4-16 parts of perilla frutescens, 2-20 parts of cyrtomium rhizomes, 10-30 parts of cod-liver oil, 2-10 parts of sodium selenite, 3-13 parts of Haixiao, 5-25 parts of radix polygalae, 2-12 parts of longan aril, 4-20 parts of Chinese yam rhizomes, 5-25 parts of vietnamese sophora roots, 3-17 parts of folium isatidis, 2-12 parts of endothelium corneum gigeriae galli, 4-16 parts of actinolitum brevifibrum, 2-12 parts of herba asari, 4-12 parts of origanum vulgare, 3-13 parts of Chinese pulsatilla roots, 4-16 parts of fleeceflower roots, 2-18 parts of radix achyranthis bidentatae, 1-9 parts of a bacillus natto agent, 2-10 parts of bacillus and 1-7 parts of active dry yeast. The cattle feed additive disclosed by the invention has the advantages of being rich in nutrition, capable of improving beef quality, increasing the survival rate of cattle and the like.

The invention relates to the technical field of protein interaction in yeast, in particular to a yeast double-expression vector, a construction method thereof, a protein interaction identification method and application. According to the vector, two exogenous genes can be cloned in the same vector, and the two exogenous genes can be expressed in yeast at the same time, so that interaction identification can be carried out. The work of plasmid construction and extraction, library preparation, yeast hybridization, screening culture and the like of an original yeast two-hybrid system is greatly reduced, and the identification efficiency of protein interaction is remarkably improved.

The invention provides a mutant of enterovirus 71 and virus-like particles thereof, and relates to the field of biological medicines. The enterovirus 71 type virus-like particle is obtained by modifying structural genes VP0, VP1 and VP3 of virus capsid protein by utilizing computational structure biology and completing self-assembly in vivo through a hansenula polymorpha expression system. Compared with the unmodified enterovirus 71 type mutant and the virus-like particles thereof, the enterovirus 71 type mutant and the virus-like particles thereof have the advantages that the immunogenicity can be obviously improved, and the clinical application prospect is relatively high.

The invention discloses a solid composite carbon source for sewage denitrification and a preparation method thereof. The composite carbon source is prepared from the following components in parts by weight: 10 to 60 parts of sodium acetate, 10 to 30 parts of glucose, 10 to 30 parts of starch, 0.1 to 2 parts of yeast powder, 1 to 5 parts of cellulose and 0.1 to 0.3 part of glycosyl hydrolase. Sodium acetate in the composite carbon source can react quickly, an instantaneous carbon source is provided, glucose and starch can be utilized by microorganisms for a long time, and durability of the carbon source is guaranteed. The invention also provides a sewage treatment method using the composite carbon source, and the method comprises the following steps: adding the composite carbon source into sewage, and carrying out denitrification treatment. The addition amount of the composite carbon source is determined based on the C / N ratio in the sewage. By adding the composite carbon source, the content of nitrate nitrogen in sewage can be effectively reduced, so that the sewage treatment efficiency is improved. The composite carbon source exists in a solid form, is convenient to store and transport, and reduces the influence on the environment. The composite carbon source not only can obviously improve the sewage treatment effect and improve the effluent quality, but also can reduce the operation cost and additional cost, and has important significance for improving the environment.

The invention discloses a recombinant strain for producing squalene as well as a construction method and application of the recombinant strain. The construction method comprises the following steps: heterologously expressing cDNA (complementary deoxyribonucleic acid) of each gene in a squalene synthesis route from filamentous fungi in yeast; the squalene synthetic pathway genes comprise a mevalonic acid-5-phosphate kinase gene, a squalene synthase gene, an acetoacetyl coenzyme A thiolase gene, a mevalonic acid kinase gene, a hydroxymethyl glutaryl coenzyme a synthetase gene, a mevalonic acid pyrophosphate decarboxylase gene, a farnesyl pyrophosphate synthetase gene and isopentenyl pyrophosphate. A dimethallylpyrophosphate isomerase gene and a truncated hydroxymethyl glutaryl coenzyme a reductase gene; the culture medium is characterized in that the filamentous fungi are Saroocladium oryzae or Stibella sp. 1056, and the culture medium is a culture medium. The excavated squalene synthesis route can complete efficient de novo synthesis of squalene, and the squalene yield is increased.

The invention belongs to the field of food processing, and particularly relates to an ostrich meat preservative and a preservation method. The ostrich meat preservative provided by the invention is prepared by matching and mixing the chitosan, the nisin and the tea polyphenol according to a specific proportion, so that the refrigeration refreshing time of the ostrich meat treated by the preservative can be effectively prolonged, the ostrich meat can maintain a relatively high L * value and a * value within 16 days of storage, the deterioration of meat color is delayed, the water-retaining property is remarkably improved, and the quality of the ostrich meat is improved. The content of thiobarbituric acid reactants and volatile basic nitrogen is obviously reduced, and fat oxidation and protein degradation are inhibited; the total number of bacterial colonies and the number of microorganisms such as coliform bacteria, pseudomonas, mould and saccharomycetes are obviously reduced. The growth and reproduction of putrefying bacteria can be effectively inhibited, and the cold fresh shelf life is prolonged.

The present invention relates to a recombinant yeast cell that produces UDP-D-glucuronic acid and / or UDP-N-acetyl-D-glucosamine compared to a wild type cell of the recombinant yeast cell; and optionally, the yield of 3 '-phosphate-adenylate sulfuric acid is increased. The invention also relates to a method for producing heparan sulfate, which comprises a step of culturing the recombinant yeast cell, further relates to an application of the non-invasive recombinant yeast cell for producing heparan sulfate, and finally relates to heparan sulfate obtained by applying the method.

The invention belongs to the technical field of microbial fermentation, and discloses torulopsis variabilis and application thereof. Wild torulopsis variabilis is separated from soy sauce mash, a strain of torulopsis variabilis with high yield of aspartic acid, threonine, glutamic acid, valine, methionine, isoleucine, leucine and lysine is obtained through ARTP mutagenesis, the collection number of the torulopsis variabilis ZB444 is GDMCC No: 65240, the classification name of the Latin name of the torulopsis variabilis is Wickhamiella versitis, the collection number of the torulopsis variabilis ZB444 is CGMCC No: 65240, and the collection number of the torulopsis variabilis ZB444 is CGMCC No: 65240. The strain is preserved in Guangdong Microbial Culture Collection Center on October 12, 2024. The strain is applied to soy sauce fermentation, the content of various amino acids and the content of total amino acids in soy sauce can be increased, the nutritional value of the soy sauce can be increased, and meanwhile the flavor of the soy sauce is enhanced.

The invention discloses Kluffa yeast and application of the Kluffa yeast in preparation of red camellia fermentation liquor with anti-aging and repairing functions, the Kluffa yeast is used for repairing PR-LQX002, the Latin name of the Kluffa yeast is Lachancea kluyveri, and the preservation number of the Kluffa yeast is CCTCC NO: M 20241723. The invention also provides a red camellia fermentation broth, a composition containing the fermentation broth and application of the red camellia fermentation broth in preparation of products with anti-oxidation, anti-saccharification, anti-wrinkle or repair effects. The fermentation broth is prepared from the following raw materials: red camellia flowers, red camellia leaves and Kluffa yeast PR-LQX002. The Kluffa yeast can greatly promote fermentation of flowers and leaves of the red camellia, and the obtained red camellia fermentation liquor not only contains active substances such as flavone, polyphenol and free amino acid, but also contains lipid substances such as ceramide, and has multiple effects such as saccharification resistance, repair, wrinkle resistance and oxidation resistance.

The present invention discloses a yeast engineering bacterium containing a (-)-α-bisabolol synthase mutant and its application. By designing (-)-α-bisabolol synthase, the present invention obtains a mutant with high expression level, co-expresses the mutant with ERG20, highly expresses the key rate-limiting enzymes in the metabolic pathway, deletes the promoter to reduce the side reaction pathway, and adopts a multi-copy strategy to improve the metabolic flux in the (-)-α-bisabolol biosynthesis pathway. The shake-flask fermentation yield of the yeast engineering bacterium constructed by the present invention is close to 70 mg / L, which is about 35 times higher than that of the starting strain, and has broad application prospects in the biological fermentation production of (-)-α-bisabolol.

The invention discloses hansenula polymorpha with low ethanol yield and high aroma yield and application of hansenula polymorpha, and belongs to the technical field of microorganisms. According to the invention, a strain of high-aroma-yield saccharomycetes, which is hanseniaspora uvarium, is separated from fruits, and the preservation number of the saccharomycetes is CCTCC (China Center for Type Culture Collection) NO: M 20242562. The quality of the low-alcohol yellow peach beverage fermented by the high-aroma-yield strain provided by the invention is improved, and the alcoholic strength after fermentation is well reduced and is only 0.4% vol. Through flavor substance detection before and after fermentation, flavor substances generated by the strain are rich in variety, for example, 90.73 mu g / kg of phenethyl alcohol is newly generated. Sensory evaluation shows that the low-alcohol yellow peach beverage fermented by the strain is outstanding in flower fragrance and fruit fragrance, the richness and the balance degree are improved, it is proved that the overall flavor characteristics of the low-alcohol beverage can be remarkably improved, and more pleasant sensory experience is brought to consumers.

The present invention relates to an RNA capping enzyme comprising or consisting of an amino acid sequence having high identity with any of the sequences of SEQ ID NO: 1-28, which can be expressed in vitro, in human cells, in mouse cells or in yeast cells, and which has the activities of RNA 5 '-triphosphatase (RT), guanylate transferase (GT) and RNA guine-N7 methyltransferase (RNMT) at the same time. The invention also relates to a mutant obtained by carrying out substitution, addition and / or deletion of amino acid on any sequence in SEQ ID NO: 1-28, a nucleotide sequence for coding the capping enzyme and / or the mutant thereof, and an expression construct or a host cell containing the nucleotide sequence. The invention also relates to a composition comprising the capping enzyme as well as a method and a reaction system for capping RNA by using the capping enzyme, a mutant or a composition thereof.

The invention relates to a protein combination for influencing the growth rate of yeast and the expression quantity of foreign proteins. After genes in a peptidase A1 family, a peptidase M1 family, a peptidase M18 family, a peptidase M28 family, a peptidase S54 family, a peptidase S10 family, a peptidase S16 family, a peptidase S14 family and a peptidase S8 family and protein defect related genes are knocked out as transcription activating factors, the growth rate can be increased, and the expression quantity of foreign proteins can be increased.

The invention belongs to the technical field of molecular biology, and particularly relates to a CeNF-YA1 gene, an expression vector and application of the CeNF-YA1 gene in vegetable fat regulation and control. The invention provides the CeNF-YA1 gene for the first time, the nucleotide sequence of the CeNF-YA1 gene is as shown in SEQ ID NO.1, the full length of a coding region of the CeNF-YA1 gene is 792 bp, a series of carriers containing the CeNF-YA1 gene, such as subcellular localization, yeast hybridization and plant overexpression, are constructed for the first time, and meanwhile, the CeNF-YA1 protein is proved to have a transcription activation function, is localized in a cell nucleus and conforms to the basic characteristics of transcription factors. It is found for the first time that overexpression of the CeNF-YA1 gene can significantly improve the grease content of arabidopsis seeds and leaves; therefore, the technical scheme provided by the invention can be used for improving the oil content of the plant seeds and the nutritional tissues, and shows the application prospect in improving the oil content of the seeds and the nutritional tissues.

The invention discloses a method for promoting synthesis of microbial oil based on a light-operated gene and a photocatalyst, and belongs to the technical field of microbial oil. According to the invention, the oleaginous yeast Po1f-OptoZWF1 containing a light-operated gene expression system is constructed by adopting a molecular biological technology, and meanwhile, the oleaginous yeast is cultured by adopting a two-stage method (darkness and illumination). In the dark stage, sufficient nutrition is provided, and rapid proliferation of the oleaginous yeast is promoted; in the grease accumulation stage, illumination culture is converted, a photocatalyst is added, a photocatalyst-oleaginous yeast hybrid system and a light-operated gene expression system are constructed to inhibit the ZWF1 gene and a corresponding pentose phosphate pathway, and a photocatalytic material provides photo-induced electrons to promote regeneration of reducing power NADPH, so that grease synthesis is efficiently promoted. According to the method, through gene modification and a two-stage culture strategy, the yield of oil synthesis is remarkably increased, and the problems that existing oil-producing microorganisms are slow in proliferation and low in oil yield are solved.

The invention discloses bacillus amyloliquefaciens which is derived from yeast for making hard liquor and has multiple tolerance and hexanoic acid production characteristics as well as a separation method and application of the bacillus amyloliquefaciens. The strain is separated from yeast for making hard liquor, can produce hexanoic acid, has the characteristics of high temperature resistance, acid resistance, ethanol resistance and the like, is identified as bacillus amyloliquefaciens DJ001, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.33299. The invention further discloses a method for preparing the caproic acid-resistant yeast for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor for making hard liquor. When the strain is added into yeast for making hard liquor, the hexanoic acid production capacity and saccharification capacity of the yeast for making hard liquor can be improved, and when the strain is added into fermented grains, the yield of white spirit and the content of ethyl hexanoate can be improved. The method has important values in the aspects of improving the yield and quality of the white spirit, saving the cost, improving the efficiency and the like, and can increase enterprise and social benefits.