39 results about "Nicotiana" patented technology

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Four genes, A622, NBB1, PMT, and QPT, can be influenced for increasing nicotinic alkaloid levels in Nicotiana plants, as well as for synthesizing nicotinic alkaloids in non-nicotine producing plants and cells. In particular, overexpressing one or more of A622, NBB1, PMT, and QPT may be used to increase nicotine and nicotinic alkaloid levels in tobacco plants. Non-nicotine producing cells can be engineered to produce nicotine and related compounds by overexpressing A622 and NBB1.

The present invention provides a nucleic acid construct comprising a cyst and root knot nematode responsive promoter, preferably the Nicotiana Ntcel7 promoter or promoters that hybridize thereto, operatively associated with a heterologous nucleic acid segment that encodes a product disruptive of nematode attack. Plants and plant cells using the same and methods of use thereof are also disclosed.

A process for producing lignin from one or more plants of genus Nicotiana is provided. The lignin can be derived inter alia from Nicotiana species biomass. A process such as is described in various embodiments herein also provides articles and compositions that include lignin produced from one or more plants of genus Nicotiana.

The present invention provides a method for producing plants with a desired phenotypic trait which comprises subjecting plants to mutagenesis, screening chimeric progeny for plants having the desired phenotypic trait, and propagating the survivors. In an embodiment, the phenotypic trait comprises an altered amino acid content. Preferably, the technique is used to generate Nicotiana tobacum plant lines having an increase in at least one amino acid. In a preferred embodiment, the invention provides improved Nicotiana tobacum plant lines producing at least 1.35 nmole of threonine per milligram of dry plant weight. These plants are useful for improving the flavor and aroma of the tobacco.

The invention provides a strain of Paenibacillus cribbus and its preparation and application, belonging to the technical field of agricultural production and biocontrol microorganisms. The preservation number of Paenibacillus cribs described in the present invention is CGMCC No.17248. The Paenibacillus kribbensis provided by the invention has significant antibacterial activity against Phytophthora nicotiana and Fusarium, and the antibacterial rate exceeds 70%. Applying the Paenibacillus cribsii provided by the invention to tobacco planting can significantly promote the growth of tobacco plants and increase the yield and value of tobacco.

The present invention provides a method for producing tobacco, or more generally, any biomass derived from any one or more species of Nicotiana (Nicotiana) or otherwise comprising The spice method of tobacco. The present invention provides spices obtained or derived from plants or plant parts from Nicotiana species, such as from one or more flowers from one or more Nicotiana species, and spices comprising one or more such spices. product.

The invention discloses bjerkandera fumosa mycelium polysaccharide and a preparation method and an application thereof. The method comprises the following steps: fermenting the material by a shake flask and collecting the bjerkandera fumosa mycelium, performing hot water extraction, ethanol deposition, freeze-drying and protein removal by an enzyme-Sevag method to obtain the mycelium de-protein polysaccharide DBFM, through a DEAE-32 cellulose anion-exchange column, separating and purifying the material to obtain the polysaccharide DBFM3 with uniform mycelium. The prepared DBFM and DBFM3 have antioxidation and immunoloregulation effects, and have application potential for preparation of an anti-oxidant and an immunoadjuvant.

The invention discloses an anti-pathogenic bacteria target gene fragment of verticillium dahlia VdEG and an interference vector and application thereof. A host induced gene silencing technology is adopted, a verticillium dahlia strain with high pathogenicity is used as a material to construct a plurality of interference plasmids of nicotiana fracture virus against exoglucanase of the target gene of verticillium dahlia, nicotiana benthamiana is transformed, and verticillium dahlia is inoculated by a method of agrobacterium injection, a Gateway interference vector is constructed in the target region which can obviously reduce the plant disease index to obtain stable genetic transgenic plants, and the fungal biomass and target gene transcription level are detected by the disease index and molecular biological methods to screen the best interference region. The screened VdEG target gene fragment and the Gateway interference vector constructed by adopting the target gene fragment can be applied to the improvement of the disease resistance of plants to verticillium dahlia and the cultivation of new varieties of anti-verticillium dahlia transgenic plants.