39 results about "Nicotiana" patented technology

The present invention provides a method for producing plants with a desired phenotypic trait which comprises subjecting plants to mutagenesis, screening chimeric progeny for plants having the desired phenotypic trait, and propagating the survivors. In an embodiment, the phenotypic trait comprises an altered amino acid content. Preferably, the technique is used to generate Nicotiana tobacum plant lines having an increase in at least one amino acid. In a preferred embodiment, the invention provides improved Nicotiana tobacum plant lines producing at least 1.35 nmole of threonine per milligram of dry plant weight. These plants are useful for improving the flavor and aroma of the tobacco.

The present invention features Nicotiana nucleic acid sequences such as sequences encoding constitutive, or ethylene or senescence induced polypeptides, in particular cytochrome p450 enzymes, in Nicotiana plants and methods for using these nucleic acid sequences and plants to alter desirable traits, for example by using breeding protocols.

Four genes, A622, NBB1, PMT, and QPT, can be influenced for increasing nicotinic alkaloid levels in Nicotiana plants, as well as for synthesizing nicotinic alkaloids in non-nicotine producing plants and cells. In particular, overexpressing one or more of A622, NBB1, PMT, and QPT may be used to increase nicotine and nicotinic alkaloid levels in tobacco plants. Non-nicotine producing cells can be engineered to produce nicotine and related compounds by overexpressing A622 and

Compositions and methods for reducing the level of nornicotine and N′-nitrosonornicotine (NNN) in Nicotiana plants and plant parts thereof are provided. The compositions comprise isolated polynucleotides and polypeptides for cytochrome P450s that are involved in the metabolic conversion of nicotine to nornicotine in these plants. Expression cassettes, vectors, plants, and plant parts thereof comprising inhibitory sequences that target expression or function of the disclosed cytochrome P450 polypeptides are also provided. Methods for the use of these novel sequences to inhibit expression or function of cytochrome P450 polypeptides involved in this metabolic conversion are also provided. The methods find use in the production of tobacco products that have reduced levels of nornicotine and its carcinogenic metabolite, NNN, and thus reduced carcinogenic potential for individuals consuming these tobacco products or exposed to secondary smoke derived from these products.

Cosmetic compositions are provided that include an extract from a seed of the Nicotiana species, one or more cosmetically acceptable carriers to act as a diluent, dispersant or carrier for the composition, and optionally one or more cosmetic adjuvants. The tobacco seed extract is typically characterized as having a significant lipid content and, thus, finds use as an oil component in a cosmetic composition.

Four genes, A622, NBB1, PMT, and QPT, can be influenced for increasing nicotinic alkaloid levels in Nicotiana plants, as well as for synthesizing nicotinic alkaloids in non-nicotine producing plants and cells. In particular, overexpressing one or more of A622, NBB1, PMT, and QPT may be used to increase nicotine and nicotinic alkaloid levels in tobacco plants. Non-nicotine producing cells can be engineered to produce nicotine and related compounds by overexpressing A622 and NBB1.

The present invention provides a nucleic acid construct comprising a cyst and root knot nematode responsive promoter, preferably the Nicotiana Ntcel7 promoter or promoters that hybridize thereto, operatively associated with a heterologous nucleic acid segment that encodes a product disruptive of nematode attack. Plants and plant cells using the same and methods of use thereof are also disclosed.

The present invention relates to p450 enzymes and nucleic acid sequences encoding p450 enzymes in Nicotiana, and methods of using those enzymes and nucleic acid sequences to alter plant phenotypes.

A process for producing lignin from one or more plants of genus Nicotiana is provided. The lignin can be derived inter alia from Nicotiana species biomass. A process such as is described in various embodiments herein also provides articles and compositions that include lignin produced from one or more plants of genus Nicotiana.

A method for producing lutein from one or more plants of genus Nicotiana is provided. The lutein can be derived inter alia from Nicotiana species biomass. A method such as is described in various embodiments herein also provides articles and compositions that include lutein produced from one or more plants of genus Nicotiana.

The invention relates to a biological pesticide composition. The biological pesticide composition comprises an A component, a B component and a C component. The A component comprises camphor, rheum officinale, chili and accessories. The B component comprises matrine, nicotiana alkaloids, aconitine, lavender essential oil, patchouli oil and perilla leaf oil. The C component comprises rhizoma atractylodes, poria cocos, dried pinellia, dried orange peel and dahurian angelica root. The weight ratio of the A component, the B component and the C component is 1:1.2-2:2.5-3. The combination of the plurality of components has a synergistic effect. The composition belongs to an insecticidal acaricidal bactericidal pesticide, and can be used for disease and pest control for vegetables, fruit trees, flowers, and the like. The composition is free of phytotoxicity for crops, free of residue, free of pollution to the environment and agricultural products, capable of not killing natural enemies, free of hazards causing abnormality, cancer and mutation for mammals, high in pest killing effects, efficient, safe and good in control effects.

The present invention relates to a method of producing a non-food crop Nicotiana plant that is optimized for producing plant-manufactured biologicals. These can be industrial or research enzymes or proteins, as well as pharmaceutical or therapeutic proteins such as vaccines, antigens, enzymes, antibodies, etc. that can be isolated and purified and administered to a subject. The invention also discloses sterile interspecific Nicotiana hybrids with high biomass produced by the method of the invention, and the seeds, tissue cultures, methods of selecting these plants and regenerating these plants.

Cosmetic compositions are provided that include an extract from a seed of the Nicotiana species, one or more cosmetically acceptable carriers to act as a diluent, dispersant or carrier for the composition, and optionally one or more cosmetic adjuvants. The tobacco seed extract is typically characterized as having a significant lipid content and, thus, finds use as an oil component in a cosmetic composition.

A method for producing lutein from one or more plants of genus Nicotiana is provided. The lutein can be derived inter alia from Nicotiana species biomass. A method such as is described in various embodiments herein also provides articles and compositions that include lutein produced from one or more plants of genus Nicotiana.

A process for producing flavorants made or derived from tobacco or, more generally, made or derived from any biomass derived from any one or more species of genus Nicotiana, or that otherwise incorporate tobacco, is provided. Provided are flavorants obtained or derived from plants or portions of plants from the Nicotiana species, such as from one or more flowers from one or more Nicotiana species, and products comprising one or more such flavorants.

The present invention provides a method for producing plants with a desired phenotypic trait which comprises subjecting plants to mutagenesis, screening chimeric progeny for plants having the desired phenotypic trait, and propagating the survivors. In an embodiment, the phenotypic trait comprises an altered amino acid content. Preferably, the technique is used to generate Nicotiana tobacum plant lines having an increase in at least one amino acid. In a preferred embodiment, the invention provides improved Nicotiana tobacum plant lines producing at least 1.35 nmole of threonine per milligram of dry plant weight. These plants are useful for improving the flavor and aroma of the tobacco.

The present invention relates to plant produced native aprotinin and aprotinin variants having enzyme-inhibitory, immunological and pharmacokinetic properties and their preparation. In a preferred method a recombinant RNA plant virus is used to express native aprotinin+variants thereof in Nicotiana plants.

Methods and compositions for genetically altered plants that produce higher amounts of at least one fatty acid compared to the amount of the fatty acid produced by a wild-type plant are provided. The genetically altered plant can be a root crop plant (e.g., sugar beet) or Nicotiana spp., or any dicot.

The invention provides a strain of Paenibacillus cribbus and its preparation and application, belonging to the technical field of agricultural production and biocontrol microorganisms. The preservation number of Paenibacillus cribs described in the present invention is CGMCC No.17248. The Paenibacillus kribbensis provided by the invention has significant antibacterial activity against Phytophthora nicotiana and Fusarium, and the antibacterial rate exceeds 70%. Applying the Paenibacillus cribsii provided by the invention to tobacco planting can significantly promote the growth of tobacco plants and increase the yield and value of tobacco.

The invention belongs to the technical field of agricultural biology, and particularly relates to a phytophthora parasitica avirulence gene PnAvr8, an encoding protein and application of the phytophthora parasitica avirulence gene. On the basis of whole genome sequencing of a phytophthora parasitica strain ASM148298v1, 17 RXLR effect factors which are remarkably up-regulated in the early infection stage are preliminarily predicted through transcriptome data of roots of disease-resistant and susceptible tobacco varieties infected by phytophthora parasitica. 17 RXLR effect factors are constructed into a PVX virus plant expression vector pGR106, and one effect factor PnAvr8 is screened from tobacco germplasm resources through a toothpick puncture method, can excite the HR reaction in a nicotiana aureoides resistant material, and cannot excite the HR reaction in common nicotiana aureoides disease-resistant and susceptible materials. The gene can be used as a resistance screening tool for breeding of tobacco resistant to phytophthora parasitica, and tobacco resources with corresponding anti-disease genes can be rapidly determined. In the process of tobacco crossbreeding and population construction, the PnAvr8 can also be used as an identification tool, so that the tobacco resistance breeding population construction is more convenient.

The present invention provides a method for producing tobacco, or more generally, any biomass derived from any one or more species of Nicotiana (Nicotiana) or otherwise comprising The spice method of tobacco. The present invention provides spices obtained or derived from plants or plant parts from Nicotiana species, such as from one or more flowers from one or more Nicotiana species, and spices comprising one or more such spices. product.

The invention discloses bjerkandera fumosa mycelium polysaccharide and a preparation method and an application thereof. The method comprises the following steps: fermenting the material by a shake flask and collecting the bjerkandera fumosa mycelium, performing hot water extraction, ethanol deposition, freeze-drying and protein removal by an enzyme-Sevag method to obtain the mycelium de-protein polysaccharide DBFM, through a DEAE-32 cellulose anion-exchange column, separating and purifying the material to obtain the polysaccharide DBFM3 with uniform mycelium. The prepared DBFM and DBFM3 have antioxidation and immunoloregulation effects, and have application potential for preparation of an anti-oxidant and an immunoadjuvant.

The invention discloses an anti-pathogenic bacteria target gene fragment of verticillium dahlia VdEG and an interference vector and application thereof. A host induced gene silencing technology is adopted, a verticillium dahlia strain with high pathogenicity is used as a material to construct a plurality of interference plasmids of nicotiana fracture virus against exoglucanase of the target gene of verticillium dahlia, nicotiana benthamiana is transformed, and verticillium dahlia is inoculated by a method of agrobacterium injection, a Gateway interference vector is constructed in the target region which can obviously reduce the plant disease index to obtain stable genetic transgenic plants, and the fungal biomass and target gene transcription level are detected by the disease index and molecular biological methods to screen the best interference region. The screened VdEG target gene fragment and the Gateway interference vector constructed by adopting the target gene fragment can be applied to the improvement of the disease resistance of plants to verticillium dahlia and the cultivation of new varieties of anti-verticillium dahlia transgenic plants.

Methods and compositions for genetically altered plants that produce higher amounts of at least one fatty acid compared to the amount of the fatty acid produced by a wild-type plant are provided. The genetically altered plant can be a root crop plant (e.g., sugar beet) or Nicotiana spp., or any dicot.

Recent advances of the research on synergistic effect of a mixed baculovirus infection demonstrated the presence of viral molecules enhancing the early event of infection. The enhancins from the Trichoplusia ni was identified to have such a function, i.e., disrupting the structural integrity of peritrophic membrane of midgut of T. ni larva. The enhancin gene was ligated downstream of the CaMV 355 promoter of a binary vector pBI121. With a drug resistant gene, the gene was introduced to a piece of tobacco leaf, from Nicotiana tobacum cv. Havana SR1. We screened 11 regenerated plants out of 37 by feeding tobacco powder mixed in artificial diet, to 3rd instar Pseudaletia separata larvae. The larval stage was usually delayed from 1 to 3 days in comparison of that of control larvae. The larvae did not pupate normally. The larvae showed irregular morphology of half larva and half pupae, suggesting a hormonal disturbance caused by the transgenic tobacco. The introduction of an enhancin gene into plants is an effective method of protecting them from insects due to the disruption of their normal life cycle.