227results about How to "Change activity" patented technology

The invention relates to the methods for modifying the methylation pattern of bacteriophage DNA and phagemid DNA and to methods for selective killing of bacteria using lysogenic bacteriophages comprising bacteriophage DNA or phagemid DNA comprising components of an engineered CRISPR-Cas system.

An object of the present invention is to provide: a novel gene (polynucleotide) encoding an FAD-conjugated glucose dehydrogenase having excellent properties that it has excellent reactivity to glucose, excellent thermal stability, and excellent substrate-recognition performance and also has a low activity for maltose; a process for the production of the enzyme using a transformant cell transfected with the gene; and a method for the determination of glucose, a reagent composition for use in the determination of glucose, a biosensor for use in the determination of glucose and others, each characterized by using the enzyme obtained. The invention relates to a polynucleotide encoding an FAD-conjugated glucose dehydrogenase, comprising a polypeptide containing an amino acid sequence: X1-X2-X3-X4-X5-X6 (wherein X1 and X2 independently represent an aliphatic amino acid residue; X3 and X6 independently represent a branched amino acid residue; and X4 and X5 independently represent a heterocyclic amino acid residue or an aromatic amino acid residue); and others.

The invention provides a family information terminal platform based on Internet of things. The platform comprises a plurality of intelligent networking modules, an Internet of things home terminal, a control terminal and a server; the intelligent networking modules are respectively embedded in applied multiple products, each intelligent networking module performs short distance two-way wireless communication with the Internet of things home terminal, and detects the working state and working parameters; the Internet of things home terminal is connected with all of the products in which the intelligent networking modules are embedded and realizes two-way communication, and further automatically controls the working mode of each product; the control terminal is connected with the intelligent networking modules and the Internet of things home terminal so as to process text, image and voice and video signals; and the server is connected with the Internet of things home terminal and a serving object through wireless and wire networks, so that a user can realize remote control to household electrical appliances. According to the platform provided by the invention, various family terminal products can be effectively integrated to realize man-machine communication; and meanwhile, the platform has the functions such as remote network management, automatic fault feedback processing of various household electrical appliances and the like.

The inventors have modified the amino acid sequence of a pullulanase to obtain variants with improved properties, based on the three-dimensional structure of the pullulanase Promozyme(R). The variants have altered physicochemical properties, e.g. an altered pH optimum, improved thermostability, altered substrate specificity, increased specific activity or an altered cleavage pattern.

A composition for delivering an agent to a cell, comprising a bispecific affinity reagent and a pH-responsive, membrane destabilizing polymer. The bispecific affinity reagent may include a first affinity reagent covalently linked to a second affinity reagent, wherein the first affinity reagent binds to a molecule on the surface of a cell, and the second affinity reagent binds to an intracellular target.

The invention relates to health tea with effects of decreasing uric acid and protecting the liver and the kidney and a preparation method thereof. The health tea is prepared from the following raw materials in parts by weight: 20 to 30 parts of kudzuvine roots, 20 to 28 parts of coix seeds, 20 to 25 parts of herba lophatheri, 15 to 25 parts of Poria cocos, 10 to 15 parts of radix glycyrrhizae, 10 to 15 parts of fresh rhizoma imperatae, 8 to 15 parts of folium mori, 5 to 10 parts of fructus lycii, 5 to 10 parts of cordyceps militaris and 1 to 2 parts of enzyme powder. The health tea has the effects of decreasing uric acid and protecting the liver and the kidney, and the functions of clearing away heat and toxic materials, decreasing uric acid and the like are achieved through the effective ingredients; the ingredients such as the herba lophatheri and Poria cocos contained in the health tea are relatively high in diuretic effects, and are absorbed by intestines and stomach when tea water enters the human body, so that the effect of neutralizing alkaline substances in blood is achieved, the alkaline substances are discharged metabolically in a urine form, urate dirty in the kidney can be cleaned, and the capacity of the kidney for filtering out uric acid is increased.

The invention discloses a preparation method of a monoatomic catalyst for indoor air purification. The preparation method comprises the following steps of (a) performing preparation to obtain a yellow solid powder CeO2 carrier; (b) dissolving RuCl3.3H2O into deionized water to obtain dipping liquid, wherein the mass concentration of ruthenium in the dipping liquid is 5 to 50 mg / mL; and (c) preparing the monoatomic catalyst: adding the CeO2 carrier obtained by the step (a) into the dipping liquid obtained by the step (b) by a dipping method, adding an iodoform solution dissolved into glycol, mixing uniformly, dipping, drying the dipped solution on a rotary evaporator and calcining to finally obtain the monoatomic catalyst Ru / CeO2. By the method, the general catalytic reaction dosage (the load capacity is very low) is extremely small, and the atom utilization rate is extremely high.

This invention relates to the vapor-phase oxidation of hydrocarbons by passing a gaseous mixture comprising of a molecular oxygen-containing gas and hydrocarbons which may contain substituents to a fixed bed of catalyst and provides a process for vapor-phase oxidation to be effected by passing a gaseous mixture of raw materials to a fixed bed of catalyst in which the void ratio of the catalyst layers increases by stages in one step or more from upstream downward in the flow of the gaseous mixture of raw materials. For example, the process can oxidize in vapor phase such hydrocarbons as naphthalene, xylene, benzene, toluene, durene, butene, acenaphthene, anthracene, indene and their derivatives in high yields with high productivity. Moreover, the process can prepare phthalic anhydride in high yields with high productivity by the vapor-phase oxidation of naphthalene or ortho-xylene.

The invention provides a catalyst of hydrogenation synthesis of ethylene glycol from dimethyl oxalate and a preparation method of the catalyst, wherein the catalyst is prepared with metal copper as a main active component, lanthanum, cerium, neodymium or samarium as additives and tetraethoxysilane as a silicon source through combination of a sol-gel method, an ammonia evaporation method and a microwave method. In the catalyst, the content of the metal copper is 5-30%, the content of the transition metal additives is 0.05-5%, and the balanced being a carrier, SiO2. The catalyst is 300-550 m<2> / g in specific surface area and is 0.4-1 cm<3> / g in pore volume. When the catalyst is used in a process of producing ethylene glycol from dimethyl oxalate through hydrogenation synthesis, the conversion rate of the dimethyl oxalate can reach not less than 99.5% and the selectivity of ethylene glycol can reach 93-97.6% at a reaction temperature of 180-190 DEG C and under a reaction pressure of 2 MPa. After a service life test for 1000 h, the average conversion rate of the dimethyl oxalate is basically maintained to be 99% and the selectivity of ethylene glycol can reach not less than 96% without any obvious decreasing tendency.

The invention discloses a preparation method of a phosphorus doped cobalt molybdate hydrogen evolution electrocatalyst and a product, and belongs to the technical field of electro-catalysis hydrogen evolution. The method comprises the following steps: (1) separately adding cobalt chloride hexahydrate, ammonium molybdate tetrahydrate and urea in deionized water, and stirring to form a uniform mixedsolution; (2) transferring the solution prepared in step (1) in a reaction kettle, adding a conductive substrate, and carrying out hydrothermal reaction to obtain beta-phase cobalt molybdate precursor material; (3) placing a sample prepared in step (2) in a tube furnace, and carrying out high-temperature thermal treatment in a nitrogen atmosphere to obtain beta-phase cobalt molybdate; and (4) carrying out phosphating on the sample prepared in step (3) in a nitrogen atmosphere to obtain phosphorus doped cobalt molybdate, wherein a reagent for phosphating is sodium hypophosphite. According to the method, by control over the amount of the sodium hypophosphite and the pyrolysis time, phosphorus is successively blended in crystal lattices of cobalt molybdate, and the prepared catalyst has efficient hydrogen evolution capability in an alkaline solution.

The invention relates to cell immobilization technology, in particular to a preparation method for immobilized bacteria used in the restoration of polycyclic aromatic hydrocarbons contaminated soil, using 0.25-1.00mm red brick powder, fly ash, vermiculite, peat soil, diatoms Soil or coke powder is used as the carrier material. After sterilization, it is inserted into the bacterial solution, and then multiplied and cultivated to obtain the desired immobilized particles. The immobilized biological product prepared according to the formula of the invention has a good remediation effect on soil polluted by high-molecular-weight polycyclic aromatic hydrocarbons, and is a reliable new environment-friendly biological product.

The invention discloses stone needle functional fibers, which consist of stone needle functional masterbatches and polypropylene fiber-grade resins, wherein the stone needle functional masterbatches comprise the following components in percentage by weight: 1 to 15 percent of stone needle powder additive, 2.5 to 9 percent of coupling agent, 15 to 25 percent of dispersing agent and 52 to 67 percent of carrier resin. A processing technology comprises two steps of preparing the stone needle functional masterbatches and performing melt spinning. The invention has the advantages that the fibers are novel functional fibers; a scientific and effective method and process is adopted, and active ingredients of stone needle are added into fibers, so that the fibers have the medial care function of the stone needle; because the active ingredients of the stone needle are added into the fibers through melt spinning, the fibers have a lasting medical care effect; and when a user wears clothing made of the fibers, the clothing have the medical care function at any time in any places, and the stone needle functional fibers are ideal health-care functional fibers.

A composition for delivering an agent to a cell, comprising a bispecific affinity reagent and a pH-responsive, membrane destabilizing polymer. The bispecific affinity reagent may include a first affinity reagent covalently linked to a second affinity reagent, wherein the first affinity reagent binds to a molecule on the surface of a cell, and the second affinity reagent binds to an intracellular target.

A composition for delivering an agent to a cell, comprising a bispecific affinity reagent and a pH-responsive, membrane destabilizing polymer. The bispecific affinity reagent may include a first affinity reagent covalently linked to a second affinity reagent, wherein the first affinity reagent binds to a molecule on the surface of a cell, and the second affinity reagent binds to an intracellular target.

The invention relates to the field of direct and controllable preparation of a metallic single-walled carbon nanotube, and in particular realtes to a growth method and application of a minor-diameter metallic single-walled carbon nanotube. A liquid nitrogen-containing organic compound is used as a carbon source and nitrogen source, hydrogen and argon are used as carrier gases, non metal silicon oxide is used as a catalyst, the single-walled carbon nanotube is grown by use of a chemical vapor phase deposition method, nitrogen acts on the catalyst in carbon nanotube nucleation and growth process, and is doped in situ into grids of the single-walled carbon nanotube, at certain temperature, single-walled carbon nanotube growth and nitrogen element doping can be simultaneously performed to obtain a minor-diameter metallic single-walled carbon nanotube network sample. The growth method realizes direct and controllable growth of the metallic single-walled carbon nanotube, the obtained minor-diameter metallic single-walled carbon nanotube network sample is free of metal contaminants, the bottleneck of direct preparing of metallic single-walled carbon nanotubes can be broken through, shortcomings of postprocessing separation methods in the prior art in obtaining of the metallic carbon nanotubes can be overcome, and the shortcomings are the problems of serious damage of the eigen structure of the carbon nanotubes and complex process.

The invention discloses expanded feed for resisting the fatty liver of tilapia and a preparation method of the expanded feed. The feed is prepared from, by mass, 3% to 8% of domestic fish meal, 15% to 25% of bean pulp, 15% to 30% of double-low rapeseed meal, 10% to 15% of cottonseed meal, 15% to 23% of strong flour, 8% to 15% of corn, 6% to 12% of DDGS, 1% to 2% of freshwater fish oil, 1% to 3% of soybean oil, 1% to 2% of monocalcium phosphate, 1% to 3% of bentonite, 1% to 2% of premix of vitamin and mineral salt, 0.1% to 0.5% of crystalline lysine, 0.1% to 0.5% of free methionine and 1% to 2% of compound fatty liver resisting preparation. Through reasonable component matching, all the nutritional requirements of tilapia are met, the stability of the feed in water is good, the tilapia grows fast, the feed conversion rate is high, and the survival rate is high. Meanwhile, according to the feed, the secretion and metabolic capability of the liver cells of the tilapia can be obviously improved, the liver function and disease resistance of the tilapia can be improved, the organ-body ratio and the fat-body ratio can be decreased, the meat taking rate can be increased, and culture benefits are good.

The present invention relates to pullulanase variants, wherein the variants have improved properties, for example, altered pH optimum, improved thermostability, altered substrate specificity, increased specific activity or altered cleavage pattern. The present invention also relates to methods of making pullulanase variants having at least one altered property based on the three-dimensional structure of a parent pullulanase.

The invention discloses elastic non-woven cloth with high water absorption and air permeability for medical magnetic therapy. The elastic non-woven cloth is made from, by weight, 20-27 parts of modified polylactic acid fiber, 20-33 parts of modified bamboo fiber, 10-20 parts of hydroxymethyl cellulose, 2-5 parts of casein, 20-30 parts of soybean fiber, 5-10 parts of sodium alginate, 20-35 parts ofstarch, 20-23 parts of magnetic starch, 40-45 parts of modified polyacrylamide fiber and 3-5 parts of a plant extract. The non-woven cloth has bacteriostatic activity, can effectively prevent wound infection, and is suitable for extensive symptoms, remarkable in health effect, safe and reliable in magnetic therapy function and economical and practical; raw materials adopted are biodegradable natural polymer materials and have good biocompatibility, and simpleness in the method is achieved.

The invention provides a trabscranial magnetoelectric depression therapeutic apparatus, comprising a pair of earphones, a beam connected between the earphones, at least a magnet, at least a pair of electrodes and an internal circuit; wherein, the earphones and the electrodes are connected with the internal circuit. The technical proposal is as follows: trabscranial magnetoelectricity and audio signals directly act on heads and auditory systems of human; stereo superposition electric field formed in certain parts of brain by trabscranial magnet can ensure the parts to generate weak induced current so as to self stimulate the parts to secrete dopamine and restore the function of self secreting neurotransmitter such as dopamine by the parts; the trabscranial magnet directly stimulates neutral clusters of basal ganglia of thalamus, thus strengthening the effects of the trabscranial magnet; the audio signals with special rhythm act on the auditory systems of human, which can promote human to enter into meditation and tempt secretion of the neurotransmitter such as dopamine in the brain, thus further strengthening the effects of the trabscranial magnet. The trabscranial magnetoelectricity and audio signals integrate three effects into one, supplement and complement each other and promote each other, and the therapeutic effect on depression and insomnia is unreachable for any single function.

The invention discloses a smart home system with an anti-theft function and an intelligent control function. The smart home system is characterized in that a central controller is connected with a fingerprint lock installed on the door panel of an entrance door and is connected with a power supply device arranged in a wall body; three infrared human body sensing sensors are adopted; the central controller is connected with the infrared human body sensing sensor arranged at the entrance door, is connected with the infrared human body sensing sensor arranged at a bedroom, and is connected with the infrared human body sensing sensor arranged at a living room; the central controller is connected with a temperature sensor arranged indoor and is connected with an alarm device arranged at the door panel of the entrance door; the central controller is connected with a camera arranged outdoor at the doorway of the entrance door and a display screen indoor side the entrance door; and the central controller is connected with a living room and bedroom air conditioning switch. The smart home system of the invention can improve reliability and is a security and protection system which is convenient to use, intelligent and has advanced functions and high reliability.

The present invention provides methods for determining the susceptibility of a pathogenic flavivirus to anti-viral compounds. This invention also provides methods for determining anti-viral drug susceptibility in a patient infected with a flavivirus. This invention also provides a method for evaluating the biological effectiveness of a candidate anti-viral drug compound. The methods are useful for identifying effective drug regimens for the treatment of flaviviral infections, and identifying and assessing the biological effectiveness of potential therapeutic compounds. Compositions including resistance test vectors and host cells transformed with the resistance test vectors are provided.

Transgenically produced decorin and methods of making and using transgenically produced decorin.

The disclosure provides, e.g., modified enucleated erythroid cells having increased or decreased levels of particular endogenous proteins. For example, CD47-negative enucleated erythroid cells may be used to induce tolerance to an exogenous antigen.

The invention relates to a pre-treatment method of a composite electrode for a TEMPO / MV flow cell. The pre-treatment method specifically comprises the following steps: firstly placing carbon electrodematerial in a domestic microwave oven (the power is at 300W to 800W) to treat for 1-10min, cleaning, and then placing in an aqueous solution containing ethidene diamine to soak for 12-24h, and then placing the soaked graphite felt in a hydrothermal reaction still containing graphene oxide and ethidene diamine mixed aqueous solution, reacting for 12-24h at 180 DEG C, freezing and drying to obtainthe electrode material for TEMPO / MV flow cell. The hydrophily of the graphite felt is increased, the binding force of the three-dimensional graphene and the graphite fiber is enhanced, the electrochemical activity of the electrode material is improved, and the energy efficiency and the voltage efficiency of the TEMPO / MV flow cell are improved.