2257results about How to "Improve fidelity" patented technology

Nucleotide triphosphate probes containing a molecular and / or atomic tag on a a γ and / or β phosphate group and / or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed.

A power transmission mechanism comprising: a flexible power transmission element; a pair of a drive pulley and a driven pulley on which the flexible power transmission element is wound, each the pulley having a pin-embedding hole formed to extend from the outer circumferential thereof toward the center thereof, and a slit elongated in the circumferential direction of the pulley to extend to opposite sides of the embedding hole and communicating with the embedding hole; and a pair of columnar or tapered anchor pins each having a path hole penetrating the anchor pin across the lengthwise direction thereof to receive the flexible power transmission element inserted therein, wherein each the anchor pin receiving the flexible power transmission element in the path hole thereof is embedded in the embedding hole of the associated pulley under pressure, and the flexible power transmission element is thereby held on the pulley.

Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars.

The invention provides methods and compositions for improving the fidelity of a sequencing-by-synthesis reaction by using a nucleotide derivative that forms a hydrogen bond with a complementary nucleotide on a template, but fails to form a phosphodiester bond with the 3′ hydroxyl group of a primer under conditions otherwise suitable for a polymerization reaction; thereby blocking incorporation of a mismatched nucleotide.

The invention provides methods for stabilizing a nucleic acid sequencing reaction. Generally, methods of the invention include exposing a target nucleic acid to a single-stranded nucleic acid binding protein and performing a sequencing reaction.

The present invention provides methods, devices and device components for fabricating patterns on substrate surfaces, particularly patterns comprising structures having microsized and / or nanosized features of selected lengths in one, two or three dimensions. The present invention provides composite patterning devices comprising a plurality of polymer layers each having selected mechanical properties, such as Young's Modulus and flexural rigidity, selected physical dimensions, such as thickness, surface area and relief pattern dimensions, and selected thermal properties, such as coefficients of thermal expansion, to provide high resolution patterning on a variety of substrate surfaces and surface morphologies.

The present invention provides methods, systems and system components for transferring, assembling and integrating features and arrays of features having selected nanosized and / or microsized physical dimensions, shapes and spatial orientations. Methods of the present invention utilize principles of ‘soft adhesion’ to guide the transfer, assembly and / or integration of features, such as printable semiconductor elements or other components of electronic devices. Methods of the present invention are useful for transferring features from a donor substrate to the transfer surface of an elastomeric transfer device and, optionally, from the transfer surface of an elastomeric transfer device to the receiving surface of a receiving substrate. The present methods and systems provide highly efficient, registered transfer of features and arrays of features, such as printable semiconductor element, in a concerted manner that maintains the relative spatial orientations of transferred features.

Disclosed is a process of screening clones having DNA from an uncultivated microorganism for a specified protein, e.g. enzyme, activity by screening for a specified protein, e.g. enzyme, activity in a library of clones prepared by (i) recovering DNA from a DNA population derived from at least one uncultivated microorganism; and (ii) transforming a host with recovered DNA to produce a library of clones which is screened for the specified protein, e.g. enzyme, activity.

Embodiments of the invention provide methods of obtaining continuous cardiac information from a mammal. First, attach a self-contained, wearable, portable continuous cardiac monitor to the mammal to create a chamber containing electrodes used to detect cardiac signals from the mammal. Next, continuously detect without analyzing the cardiac signals from the mammal for at least 24 hours. Next, store information related to substantially all detected cardiac signals in the cardiac monitor.

An electrophysiology medical probes, which may be incorporated into a system and used to perform an electrophysiology procedure, is provided. The medical probe comprises an elongated member (e.g., a flexible elongated member), and a metallic electrode mounted to the distal end of the elongated member. In one embodiment, the metallic electrode is cylindrically shaped and comprises a rigid body. The medical probe further comprises a plurality of microelectrodes (e.g., at least four microelectrodes) embedded within, and electrically insulated from, the metallic electrode, and at least one wire connected to the metallic electrode and the microelectrodes.

Non-intrusive peripheral systems and methods to track, identify various acting entities and capture the full motion of these entities in a sports event. The entities preferably include players belonging to teams. The motion capture of more than one player is implemented in real-time with image processing methods. Captured player body organ or joints location data can be used to generate a three-dimensional display of the real sporting event using computer games graphics.

A system and method lets a user create or import ontologies and create databases and related application software. These databases can be specially tuned to suit a particular need, and each comes with the same error-detection rules to keep the data clean. Such databases may be searched based on meaning, rather than on words-that-begin-with-something. And multiple databases, if generated from the same basic ontology can communicate with each other without any additional effort. Ontology management and generation tools enable enterprises to create databases that use ontologies to improve data integration, maintainability, quality, and flexibility. Only the relevant aspects of the ontology are targeted, extracting out a sub-model that has the power of the full ontology restricted to objects of interest for the application domain. To increase performance and add desired database characteristics, this sub-model is translated into a database system. Java-based object-oriented and relational application program interfaces (APIs) are then generated from this translation, providing application developers with an API that exactly reflects the entity types and relations (classes and methods) that are represented by the database. This generation approach essentially turns the ontology into a set of integrated and efficient databases.

The virtual companion described herein is able to respond realistically to tactile input, and through the use of a plurality of live human staff, is able to converse with true intelligence with whomever it interacts with. The exemplary application is to keep older adults company and improve mental health through companionship.

A noise suppression device receives data representative of a noise-corrupted signal which contains a speech signal and a noise signal, divides the received data into data frames, and then passes the data frames through a pre-filter to remove a dc-component and the minimum phase aspect of the noise-corrupted signal. The noise suppression device appends adjacent data frames to eliminate boundary discontinuities, and applies fast Fourier transform to the appended data frames. A voice activity detector of the noise suppression device determines if the noise-corrupted signal contains the speech signal based on components in the time domain and the frequency domain. A smoothed Wiener filter of the noise suppression device filters the data frames in the frequency domain using different sizes of a window based on the existence of the speech signal. Filter coefficients used for Wiener filter are smoothed before filtering. The noise suppression device modifies magnitude of the time domain data based on the voicing information outputted from the voice activity detector.

A system and process is provided for the conversion of a stream of two-dimensional images into a pair of streams of images for providing the perception of a stream of three-dimensional images. Each complimentary image in the image stream undergoes the application of a selection and remapping process to independently alter portions of the image, so that the remappings shift the image elements in a manner which produces a stereo depth effect when the images are viewed through the appropriate viewing device.

The present invention provides methods, devices and device components for fabricating patterns on substrate surfaces, particularly patterns comprising structures having microsized and / or nanosized features of selected lengths in one, two or three dimensions and including relief and recess features with variable height, depth or height and depth. Composite patterning devices comprising a plurality of polymer layers each having selected mechanical and thermal properties and physical dimensions provide high resolution patterning on a variety of substrate surfaces and surface morphologies. Gray-scale ink lithography photomasks for gray-scale pattern generation or molds for generating embossed relief features on a substrate surface are provided. The particular shape of the fabricated patterned can be manipulated by varying the three-dimensional recess pattern on an elastomeric patterning device which is brought into conformal contact with a substrate to localize patterning agent to the recess portion of the pattern.

The invention comprises a totally implantable hearing prosthesis for hearing impaired persons. An inertial vibrational element is hermetically sealed and implanted in bone between the lateral and superior semicircular canals without breaching the integrity of the canals. The vibrational element is adapted to vibrate the walls of the canals and the fluids contained therein, thereby vibrating contiguous fluids within the cochlea thus stimulating hair cells and creating a hearing percept. The invention can also be adapted to be a tinnitus masking system, and / or used in combination with a coehlear implant hearing system.

Embodiments of the present invention provide a method of analyzing cardiac information by collecting a plurality of self-contained, wearable, portable cardiac monitors each of the cardiac monitors electronically storing at least 24 hours of continuously detected and unanalyzed cardiac signals from a mammal. Next, retrieving cardiac information stored in each of the plurality of self-contained portable cardiac monitors. Next, forwarding retrieved cardiac information.

Graphic displays, which display information about process elements and the manner in which these elements are connected within a process, process modules, which simulate the operation of the elements depicted within the graphic displays and control modules, which perform on-line control activities within a process, may be communicatively connected together to provide a combined control, simulation and display environment that enables enhanced control, simulation and display activities. Smart process objects, which have both graphical and simulation elements, may used to create one or more graphic displays and one or more process simulation modules, each having elements which may communicate with one another to share data between the graphic displays and the process modules. Additionally, function blocks within control modules executed in the process plant may reference the elements within the graphic displays and the process modules (and vice versa) so that control modules may use simulated data developed by the process modules to perform better control, so that process modules may perform better simulation using actual pant data from the control modules, and so that the graphic displays may be used to illustrate actual process data and / or simulated process data as developed by the control modules and the process modules.

Disclosed herein is a system and method for increasing the fidelity of measured genetic data, for making allele calls, and for determining the state of aneuploidy, in one or a small set of cells, or from fragmentary DNA, where a limited quantity of genetic data is available. Genetic material from the target individual is acquired, amplified and the genetic data is measured using known methods. Poorly or incorrectly measured base pairs, missing alleles and missing regions are reconstructed using expected similarities between the target genome and the genome of genetically related individuals. In accordance with one embodiment of the invention, incomplete genetic data from an embryonic cell are reconstructed at a plurality of loci using the more complete genetic data from a larger sample of diploid cells from one or both parents, with or without haploid genetic data from one or both parents. In another embodiment of the invention, the chromosome copy number can be determined from the measured genetic data of a single or small number of cells, with or without genetic information from one or both parents. In another embodiment of the invention, these determinations are made for the purpose of embryo selection in the context of in-vitro fertilization. In another embodiment of the invention, the genetic data can be reconstructed for the purposes of making phenotypic predictions.

Digital filters for processing an input audio signal for extending the audio capabilities and reducing distortion of a loudspeaker. Such extension may include a general sound pressure level (SPL) extension or a more targeted low frequency (LF) extension. This may be accomplished by modifying an original frequency response of the loudspeaker to achieve a particular extension, and applying a digital linear filter based on the modified frequency response to the audio signal. A nonlinear digital filter may also be applied to the audio signal reduce nonlinear distortions of the loudspeaker. The nonlinear digital filter may be based on an inverse of an electro-mechanical model of the loudspeaker. In this manner, a loudspeaker may be driven close to its maximum theoretical power capability without increasing distortion in the loudspeaker output.

Payment-based audiovisual playback system characterized by comprising a microprocessor device associated with a payment device primarily including means for storing, inter alia, in digital format the visual and sound information to be used. The system is associated through interfaces with display means and sound playback means for providing a multimedia environment. The system is controlled by a multitask operating system including a tool and service library integrated into the storage means. The system, which is also associated through an interface with a telecommunications modem, is optionally connected to an audiovisual data distribution network by a telecommunications modem and telecommunications links, said telecommunications function also being controlled by said multitask operating system.

Payment-based audiovisual playback system characterized by comprising a microprocessor device associated with a payment device primarily including means for storing, inter alia, in digital format the visual and sound information to be used. The system is associated through interfaces with display means and sound playback means for providing a multimedia environment. The system is controlled by a multitask operating system including a tool and service library integrated into the storage means. The system, which is also associated through an interface with a telecommunications modem, is optionally connected to an audiovisual data distribution network by a telecommunications modem and telecommunications links, said telecommunications function also being controlled by said multitask operating system.

An apparatus and that provide an optical-fiber amplifier having at least one erbium-doped fiber section and an optical pump coupled to the erbium-doped fiber section, wherein the apparatus is operable to amplify signal pulses to high energy in the erbium-doped fiber section, the pulses having a wavelength in the range of about 1565 nm to about 1630 nm. In some embodiments, the amplifying fiber is ytterbium free.

Methods of fabricating a semiconductor device are provided. Methods of forming a finer pattern of a semiconductor device using a buffer layer for retarding, or preventing, bridge formation between patterns in the formation of a finer pattern below resolution limits of a photolithography process by double patterning are also provided. A first hard mask layer and / or a second hard mask layer may be formed on a layer of a substrate to be etched. A first etch mask pattern of a first pitch may be formed on the second hard mask layer. After a buffer layer is formed on the overall surface of the substrate, a second etch mask pattern of a second pitch may be formed thereon in a region between the first etch mask pattern. The buffer layer may be anisotropically etched using the second etch mask pattern as an etch mask, forming a buffer layer pattern. The second hard mask layer may be anisotropically etched using the first etch mask pattern and / or the buffer layer pattern as etch masks, forming a second hard mask pattern. The first hard mask layer may be anisotropically etched using the second hard mask pattern as an etch mask, forming a first hard mask pattern. The etched layer may be anisotropically etched using the first hard mask pattern as an etch mask.

A video coding algorithm supports both lossy and lossless coding of video while maintaining high color fidelity and coding efficiency using an in-loop, reversible color transform. Accordingly, a method is provided to encode video data and decode the generated bitstream. The method includes generating a prediction-error signal by performing intra / inter-frame prediction on a plurality of video frames; generating a color-transformed, prediction-error signal by performing a reversible color-space transform on the prediction-error signal; and forming a bitstream based on the color-transformed prediction-error signal. The method may further include generating a color-space transformed error residual based on a bitstream; generating an error residual by performing a reversible color-space transform on the color-space transformed error residual; and generating a video frame based on the error residual.

A portable system capable of receiving audio signals from one or more sources and reproducing the signals via speakers contained therein. The portable audio reproduction system can include a docking station or similar means for communicatively coupling an audio device to the portable audio reproduction system, thereby reducing desktop clutter and simplifying connecting the portable audio reproduction system with the audio device. The speakers of the portable audio reproduction system can preferably be received within a base portion of the system, thereby protecting the speakers during transport and reducing the overall size of the system for improved portability. External connections may also be provided, thereby enabling the portable audio reproduction system to receive audio signals from additional sources, and a mixer allows the system to reproduce signals from a portable audio device and an auxiliary source, such as a computer.

The present invention provides a system and method of modifying the mask layout shapes of an integrated circuit layout design to compensate for reticle field location-specific systematic CD variations resulting from mask writing process variations, lens imperfections in lithographic patterning, and photoresist process variations. Called PLC (Process-optimized Layout Compensation), each set of compensation rules according to the present invention is specifically tailored for a particular mask-writer-patterning-tools-and-resist-process combination, and are performed on a reticle-wide basis. Furthermore, for each geometric shape in the mask layout, the amount of modification is determined based on a categorization of the type of the shape, the specific location in the reticle field the particular shape falls in, its context (i.e., surrounding patterns, orientation, etc.), as well as certain photoresist parameters to be used in the patterning process.