The application discloses a deep-sea in-situ
macro-
transcriptome RNA multi-stage
processing and reverse transcription stabilization method and
system, relates to the technical field of deep-sea
biology and marine instrument equipment, and has the following components in series in a continuous closed fluid channel: a
filtration enrichment cavity, a controlled decompression unit, a
program control unit and at least one multifunctional reaction cavity. A
temperature control system is integrated in the multifunctional reaction cavity, at least one set of bidirectional opposite
electromagnet groups is arranged on the outer side of the
cavity wall of the multifunctional reaction cavity, the
program control unit is used for controlling the on-off and current of the bidirectional opposite
electromagnet groups, and a dynamically switchable spatial
magnetic field gradient is generated in the multifunctional reaction cavity to drive magnetic microspheres in the cavity to perform at least one operation, such as adsorption and aggregation, suspension and mixing, in-situ cleaning, transfer between cavities and
elution and
desorption, without relying on
centrifugation. All biochemical operations of the application are completed in the continuous sealed fluid channel, cross
contamination of samples and external environment is eliminated, and full-automatic
sample processing under a deep-sea environment is realized.