The invention discloses a method for maintaining continuous photosynthetic
hydrogen production of microalgae under ultrahigh
light intensity, and belongs to the technical field of biological energy sources. The method comprises the following steps: culturing microalgae: transferring microalgae species into a TAP culture medium, and carrying out illumination culture until the number of microalgae cells reaches a logarithmic phase; the preparation method comprises the following steps: synthesizing a poly (N-isopropylacrylamide-
butyl acrylate)
copolymer, namely dissolving N-isopropylacrylamide,
butyl acrylate and an azodiisobutyronitrile solution in
anhydrous tetrahydrofuran, removing
oxygen for 30 minutes under the action of magnetic stirring, and reacting for 24 hours at the temperature of 50 DEG C after the
oxygen removal is finished, so as to obtain PNIPAM-BA (poly (N-isopropylacrylamide-
butyl acrylate)-BA (poly (N-isopropylacrylamide-butyl
acrylate))-BA (poly (N-isopropylacrylamide)-BA); the method comprises the following steps: taking a microalgae solution, centrifugally collecting microalgae, dispersing the microalgae in an HM culture medium, adding PNIPAM-BA and
graphene oxide, and continuously illuminating at 25 DEG C under the
light intensity of 2000 [mu] mol photons.m <-2 >. S <-1 >; the
sunlight-driven intelligent microalgae photoreactor is constructed through
graphene oxide and PNIPAM-BA, the
survival rate and
chlorophyll content of microalgae under the ultrahigh
light intensity can be maintained, and the microalgae can maintain high-rate photosynthetic
hydrogen production under the ultrahigh light intensity of 2000 micromoles photons * m <-2 > * s <-1 >.