637 results about "Drought resistance" patented technology

The invention relates to the technical field of mountain afforestation, provides an efficient nursery stock planting method suitable for mountain afforestation, and aims at solving the problems that in existing mountain afforestation, planting is not standard, the survival rate is low, the water and soil conservation capacity is weak, and the labor intensity is high. The forest space layout is optimized, and the water and soil conservation capability is enhanced; the mellow soil and immature soil layered stacking and backfilling technology is adopted, the natural structure of the soil is recovered, and the root zone microenvironment quality is improved; a fertilizer isolation layer is arranged and degradable container seedlings are implanted, so that the risk of root burning is avoided, the integrity of root systems is protected, and the survival rate of the seedlings is remarkably increased; by combining root fixing water slow irrigation, water retention covering and slope water collection and soil conservation structures, natural rainfall is effectively utilized, evaporation and runoff are reduced, and water saving and drought resisting are achieved; the whole process flow is standard, the replicability is high, and the dependence on artificial experience is reduced.

The invention belongs to the field of plant genetic engineering, and discloses application of a PtNF-YB9 gene of trifoliate orange in drought-resistant genetic improvement of plants. The PtNF-YB9 gene is a transcription factor gene which is separated and cloned from Poncirus trifoliata, and the sequence of the PtNF-YB9 gene is as shown in SEQ ID NO. 1. The gene or the homologous gene thereof is knocked out or inhibited in a plant, the drought resistance of the plant is remarkably reduced, and the drought resistance of the plant is remarkably enhanced by overexpressing the gene or the homologous gene thereof. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing the green and environment-friendly agricultural goal.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying drought tolerance of wheat, haplotypes, primers and application. Belongs to the technical field of biological breeding. Research finds that three SNP loci exist on a wheat ARF20-B gene, a wheat drought-enduring haplotype ARF20-BHapI is developed based on the SNP loci, the first locus is located at the 3616bp position of the ARF20-B gene, and the A / G nucleotide polymorphism is A; the second site is located at the 5623bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism; the third site is located at the 9795bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism. The wheat carrying the ARF20-BHapI haplotype has the advantage that the drought tolerance of the wheat carrying the ARF20-BHapI haplotype is higher. The invention further provides a KASP primer group for detecting the ARF20-BHapI haplotype, and successful detection of the drought tolerance of the wheat is achieved.

The invention discloses a comprehensive evaluation method for drought resistance of corn varieties, which relates to the technical field of corn variety evaluation, and comprises the following steps: obtaining multi-temporal multi-band reflection spectrum data under the condition of continuous drought of corn, and performing structure coupling modeling on the spectrum data in combination with the structural characteristics of corn leaf epidermis wax, so as to obtain the drought resistance of the corn varieties; constructing a time sequence curve capable of representing the dynamic change of leaf wax distribution as a dynamic spectrum monitoring baseline; differential analysis between continuous time points is carried out based on the dynamic spectrum monitoring base line, spectrum abrupt change points caused by leaf wax migration are identified, and space consistency inspection is combined. According to the method, a dynamic spectrum monitoring baseline and an energy migration fingerprint are constructed, wax migration interference is accurately identified, a spectrum stability hypothesis is broken through, high precision, self-adaption and consistency of evapotranspiration rate inversion under a drought condition are realized through multi-level correction and a closed-loop updating strategy, and scientificity and comparability of drought resistance evaluation are improved.

The invention provides application of an OsRTD1 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsRTD1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the OsRTD1 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of the OsRTD1 gene mutant plant osrtd1 under the drought stress of PEG induction and soil dehydration is remarkably improved, the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced, the relative water loss rate of dehydrated leaves is higher, and the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced. The total grain number, the solid grain number, the total grain weight and the like of the OsRTD1 gene mutant plant osrtd1 under the stress of field drought are obviously higher than those of a wild plant. The OsRTD1 gene is a potential candidate new target for regulating and controlling a rice drought resistance mechanism.

The invention discloses a method for inducing infiltration of root systems of sandy vegetation. The method comprises the steps of land selection and preparation, pit digging, vegetation selection, arrangement of a PVC pipe water applying system, vegetation planting and backfilling and layered water control operation. According to the method, PVC pipes with water seepage holes with different depths are arranged near the root system of vegetation, a self-descending and lifting piston structure of a water-absorbing expansion cylindrical block and a depth-keeping hook-hanging ring chain is adopted in the pipes for the first time, and the distance between the water seepage holes, the length of a rope section and the size of a hanging ring are accurately calculated, so that it is guaranteed that the distance between the water seepage holes and the rope section is accurately calculated at each stage; water can only seep from the water seepage holes with the specific target depth, the root system is guided to follow a water signal to be deeply pricked downwards continuously, a deep and developed root system is formed, accurate layered water control and root system directional induction are achieved, the drought resistance and wind falling resistance of vegetation are greatly enhanced, the water consumption is low, the vegetation survival rate is high, and the growth vigor is good; the depth-keeping water applying system serves as a multifunctional and permanent ecological restoration unit and further has the functions of soil ventilation, humidification and self-cleaning.

The invention relates to the field of plant molecular biology and forest genetic engineering, and particularly provides a PagRAP2.3 protein point mutant and application thereof in drought resistance of poplar. The mutant PagRAP2.3 MA is obtained by mutating methionine at the first site and cysteine at the second site of a wild type PagRAP2.3 protein into methionine and alanine. The invention further discloses a preparation method of the mutant PagRAP2.3. An expression vector containing the PagRAP2.3 MA gene is constructed, poplar 84K is transformed through an agrobacterium-mediated method, and a transgenic line with stable expression is obtained. Functional verification results show that overexpression of PagRAP2.3 MA can significantly increase the plant height of the poplar and promote plant growth, but the sensitivity to moisture is enhanced under drought stress. The mutant can be used for regulating and controlling the growth and development of forest trees and evaluating the drought resistance, and has a forestry breeding application prospect.

The invention relates to the technical field of biological genetic engineering, in particular to application of a brassica napus BnaA01G0331000ZS gene in drought stress resistance. The BnaA01G0331000ZS gene is cloned by using cDNA of double 11 leaves as a template through a sequence published in a brassica napus genome database, an over-expression vector is constructed and genetically transformed into rape variety high oil for transgenic function verification, the drought resistance of the obtained over-expression transgenic line is obviously enhanced compared with that of a wild type, and the BnaA01G0331000ZS gene has the advantages that the BnaA01G0331000ZS gene is obtained; therefore, the gene can positively regulate the drought stress resistance of the rape. Therefore, the invention provides a new gene resource for adversity stress resistance of rape.

The invention provides application of an OsERF8 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsERF8 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the encoding protein of the OsERF8 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of an oserf8 mutant plant under PEG induction and soil dehydration drought stress is remarkably improved; and the survival rate of the overexpressed plant OsERF8-OE under the induction of PEG (Polyethylene Glycol) is obviously reduced. Compared with a wild type plant, after PEG induces drought stress, the malondialdehyde content of the overground part of the oserf8 mutant plant is reduced, and the proline content is increased; the malondialdehyde content of the overground part of the overexpressed plant OsERF8-OE is increased, and the proline content is reduced. In conclusion, the OsERF8 gene is a potential candidate new target for researching a rice drought resistance regulation mechanism.

The invention discloses application and a method of wheat demethylase TaALKBH23 in regulation and control of drought stress resistance of plants, a coding sequence of the demethylase TaALKBH23 is shown as SEQ ID NO: 1, and an amino acid sequence coded by the demethylase TaALKBH23 is shown as SEQ ID NO: 2. The invention provides demethylase TaALKBH23 and a function and application of a coding gene of the demethylase TaALKBH23 in regulation and control of plant drought resistance, and it is found through construction of a TaALKBH23 wheat genetic transformation material that the gene negatively regulates and controls wheat drought resistance. It is found that TaALKBH23 plays an important role in the process of regulating and controlling drought stress resistance of plants, and a new gene resource is provided for improving drought resistance of crops.

The invention belongs to the technical field of image processing, and discloses a rice bud stage drought resistance prediction method, which comprises the following steps: acquiring sample images of a plurality of individuals of a rice variety to be detected under control and stress conditions, performing foreground segmentation and structural region marking on the images, and identifying seed, bud and root regions. And multi-dimensional phenotypic features are extracted for each region to construct a single-sample high-dimensional feature vector. And carrying out group feature extraction on a plurality of single sample vectors under the same processing condition, and carrying out double correction on a sample confidence coefficient weight and a feature steady-state weight to obtain a group feature vector. And constructing a multi-dimensional drought resistance response feature vector according to the difference between the group feature vectors of the stress group and the control group, and inputting the multi-dimensional drought resistance response feature vector into a pre-trained classification model to obtain a drought resistance level prediction result. The method achieves the automation of drought resistance evaluation, effectively inhibits the interference of abnormal samples, and improves the evaluation accuracy.

The invention discloses a gene PtoXTH30 capable of improving drought resistance and salt resistance of poplar and application of the gene PtoXTH30, the nucleotide sequence of the gene is shown as SEQ ID NO.2, the gene can accurately regulate and control the drought resistance and salt resistance of the poplar, and a key candidate gene is provided for stress-resistant molecular breeding of the poplar. The invention further discloses application of the PtoXTH30 gene for improving drought resistance and salt resistance of the poplar, and the drought resistance and salt resistance of the poplar can be remarkably enhanced. The invention further discloses a method for regulating and controlling drought resistance and salt resistance of the poplar, the drought resistance and salt resistance of the poplar can be accurately regulated and controlled according to needs, the breeding period is effectively shortened, and breeding of new drought-resistant and salt-resistant transgenic varieties is facilitated.

The invention discloses a sugarcane ScPP2C49 gene and application thereof, and relates to the technical field of plant biology, the nucleotide sequence of the ScPP2C49 gene is as shown in SEQ ID No.1, and the amino acid sequence of protein coded by the ScPP2C49 gene is as shown in SEQ ID No.2. The gene is induced to express by drought and abscisic acid, and the encoded protein of the gene is positioned in a cell nucleus. Functional studies show that after overexpression of the ScPP2C49 gene in the plant, the drought resistance of the plant is negatively regulated through various mechanisms of inhibiting an abscisic acid signal channel, hindering stomatal closure under drought stress, weakening active oxygen scavenging ability, reducing photosynthetic efficiency, inhibiting root growth and the like. The invention also provides a recombinant overexpression vector containing the gene and a method for obtaining a transgenic plant with reduced drought resistance by using the vector through an agrobacterium-mediated method. According to the invention, a new gene resource is provided for deep analysis of a plant drought-resistant molecular mechanism, and an important target gene is provided for cultivation of high-stress-resistance crop varieties through a reverse genetics means (such as gene knockout).

The invention discloses an urban drainage system multi-node cooperative control method based on multi-objective optimization, and relates to the technical field of drainage, and the method comprises the steps: obtaining a water loss coefficient of an urban basic block; analyzing to obtain a water level upper limit and a water level lower limit of the urban basic block; respectively forming a first water storage plot set and a second water storage plot set of the urban basic blocks; analyzing to obtain the water level variation of the city basic block on the day; determining management attributes of the urban basic blocks; forming a drainage set of the urban basic blocks; and forming a drought-resistant set of the urban basic blocks. By obtaining the water loss coefficient of the urban basic block, obtaining the water level variation of the urban basic block on the day and forming the flood drainage set and the drought resistance set of the urban basic block, various factors of planting in each city are considered in a targeted mode, the selected scheme can meet the drought resistance and flood drainage requirements to a large extent, and therefore the method has the advantages that the planting efficiency is improved, and the planting cost is reduced. And the urban drainage system is controlled through the greening vegetation.

The invention discloses a Chimonobambusa chensinensis CuCCoAOMT1 gene as well as a protein and application thereof, belongs to the technical field of plant genetic engineering, and particularly relates to a Chimonobambusa chensinensis caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene, and a nucleotide sequence of the CuCCoAOMT1 gene is shown as SEQ ID NO.1. The invention further discloses a preparation method of the CuCCoAOMT1 gene. The invention further discloses application of the CuCCoAOMT1 gene in regulation and control of the drought resistance of gramineous plants. By overexpressing the caffeoyl-coenzyme A-O-methyltransferase CuCCoAOMT1 gene in the rice, the lignin content in the rice can be effectively increased, the thickening of rice cell walls is promoted, the drought resistance of rice plants is improved, and a new gene resource is provided for plant material property improvement gene engineering.

The invention belongs to the technical field of biotechnology, and relates to a wheat drought-resistant related protein TaELF4 as well as a coding gene and application thereof. A recombinant vector p110UBI-R containing a TaELF4 gene CDS sequence of SEQ ID NO.1 is used for transforming wheat, the survival rate of an obtained T3-generation transgenic line is remarkably higher than that of wild type wheat under the drought condition, the yield is averagely increased by about 15%, and the yield is statistically remarkably higher than that of the wild type wheat. The invention has important significance in molecular breeding for regulating and controlling the drought resistance and yield of wheat.

The invention discloses a ZmRIPK2 protein and application of a coding gene thereof in plant drought resistance, the amino acid sequence of the ZmRIPK2 protein is shown as SEQ ID No.1, and the nucleotide sequence of the coding gene of the protein is shown as SEQ ID No.2. After the ZmRIPK2 gene is knocked out from a plant and drought stress treatment is carried out, the drought resistance of a mutant plant is improved compared with that of a control group. The invention further discloses a method for improving the drought resistance of the corn, the ZmRIPK2 gene is knocked out from a receptor plant, and the corn with high drought resistance is obtained. The invention verifies that the ZmRIPK2 protein and the coding gene thereof have a negative regulation function on the drought resistance of the corn, and an excellent candidate gene resource is provided for cultivating and improving a new variety of drought-resistant corn; according to the provided drought-resistant plant breeding method, the breeding process is more efficient, reliable, accurate and controllable, and the method has important theoretical and practical significance for improving crop resistance and accelerating the corn breeding process.

The invention provides an InDel molecular marker sequence related to wheat drought resistance as well as a detection method and application thereof, and belongs to the technical field of molecular markers. The invention provides a polymorphic site for forming different drought resistance of wheat, the polymorphic site only has two haplotypes: a sequence of a 724bp long fragment as shown in SEQ ID No.2 is inserted between the fragments 245-246bp as shown in SEQ ID No.1, and the haplotype is homozygous as a haplotype A; the drought resistance of to-be-detected wheat which is not inserted with the 724bp long fragment sequence shown at the site, is haplotype B homozygous and is determined to be haplotype A homozygous is higher than that of a variety determined to be haplotype B homozygous, so that the drought resistance of the to-be-detected wheat is determined to be haplotype A homozygous by detecting the condition of the polymorphic site when the wheat is subjected to molecular marker-assisted selective breeding, and the drought resistance of the to-be-detected wheat is determined to be haplotype A homozygous. The wheat with relatively high drought resistance can be found.

The invention discloses a breeding method of a drought-resistant machine-harvested cotton variety. The breeding method comprises the following steps: step A1, hybridizing multiple parents; the method comprises the following steps: constructing a basic hybrid combination by taking a cotton line with strong drought resistance as a female parent and a cotton line with outstanding mechanical-harvest character as a male parent, introducing a wild cotton germplasm and a high-quality fiber cotton germplasm as innovative parents, and polymerizing drought-resistant, mechanical-harvest-suitable, high-quality fiber and stress-resistant related genes through composite hybridization by combining distant hybridization with an embryo rescue technology to obtain F1-generation seeds; the invention relates to the technical field of cotton breeding. According to the breeding method for the drought-resistant machine-harvested cotton variety, parent combinations are scientifically configured, a stable drought-resistant line and a machine-harvested line are used for constructing a basic hybrid combination, meanwhile, a wild cotton stress-resistant germplasm and a high-quality fiber cotton germplasm are introduced, and a quaternary composite hybridization and special embryo rescue technology is innovatively adopted; the seedling rate of the distant hybridization embryo of the wild cotton is increased to 60% or above, and the problem of abortion of the distant hybridization embryo is effectively solved.

The invention provides an application of a nitrification inhibitor and biochar as a soil conditioner in enhancing drought resistance of plants, a preparation method of the biochar is as follows: moso bamboo stalks are taken as raw materials, pyrolysis is performed for 4-8 hours under an anoxic state of 250-400 DEG C, soil is red soil, and the plant is polygonatum cyrtonema, and an application method comprises the following steps: adding a nitrogen fertilizer into the soil, the addition amount of the nitrification inhibitor is 1% of the content of nitrogen added to the soil, and the addition amount of the biochar is 3% of the mass of the soil. Through combined application of the biochar and the dicyandiamide, the drought resistance of the polygonatum cyrtonema and the benefits of soil microbial communities are remarkably improved.

The invention relates to the technical field of plant genetic engineering, in particular to a ZmbHLH166 gene related to drought resistance in the flowering stage of corn, an encoding protein and application of the ZmbHLH166 gene. The key effect of the ZmbHLH166 gene in the drought resistance of the corn in the flowering period is identified for the first time on the basis of whole genome association analysis (GWAS) and transgenic function verification, and through experiments, through genetic transformation and continuous selfing screening of a Ubi1: ZmbHLH166 overexpression vector, the obtained T2-generation corn strain of the overexpression ZmbHLH166 gene has the advantages that compared with a wild type corn strain, the yield is increased, and the yield is increased. The ZmbHLH166 gene has a shorter pollen scattering-spinning interval, which indicates that under drought stress, the overexpression of the ZmbHLH166 gene can effectively promote the synchronous development of male and female flowers of corn, thereby enhancing the drought resistance of crops. Therefore, the application of the ZmbHLH166 gene and the protein coded by the ZmbHLH166 gene in regulating and controlling the drought resistance of the corn in the flowering period is feasible, and a new and reliable gene resource and an improvement direction are provided for drought-resistant breeding of the corn.

The invention discloses a primer combination for water saving and quality screening of winter wheat and application thereof, and relates to the technical field of molecular biology, the primer combination for water saving and quality screening of winter wheat comprises a primer pair P1 and a primer pair P2; according to the primer combination, the water-saving and drought-resistant characters are screened through PCR amplification, and SDS-PAGE electrophoresis is combined to detect grain protein subunit composition so as to screen quality characters. The efficient breeding method suitable for winter wheat is formed by designing a specific primer combination, constructing a molecular marker screening system and innovatively combining a cultivation management mode of water before drought and drought after water before winter wheat. According to the method, dependence on a single known gene is avoided, oriented improvement of water-saving drought resistance and quality is realized through collaborative screening and environment adaptability cultivation of a primer combination, and a new technical path is provided for wheat breeding in the arid region of North China.

The invention discloses application of an FYVE4 gene in improving nitrogen deficiency nutrition stress tolerance of plants. Belongs to the technical field of plant genetic engineering. The invention provides nucleotide and corresponding amino acid sequences and specific application thereof. The invention proves for the first time that the arabidopsis thaliana gene AtFYVE4 regulates the nitrogen deficiency stress response of the plant through an autophagy way. It is found that the arabidopsis thaliana nitrogen deficiency nutrition stress regulation related gene regulates the autophagy activity of arabidopsis thaliana through interaction with the autophagy protein AtATG1C, and then regulates the tolerance of plants to nitrogen deficiency nutrition stress. The application of the AtFYVE4 gene can provide support for drought resistance research of grain crops such as corn, rice, wheat and the like and other crops.

The invention discloses a waterlogging-resisting, seedling-protecting, drought-resisting and harvesting-protecting planting method for oilseed rape in a rice field, and belongs to the technical field of crop planting. (2) after the rice is harvested, when a tractor does not sink during operation, selecting a sunny day to crush the straws, and naturally and uniformly dispersing the crushed straws in the field; (3) when there is no open water in the field and the tractor is not trapped during operation, turning over once by using the tractor, and carrying out rotary tillage twice so as to uniformly mix the straws and the soil; (4) after the rotary tillage operation is completed, ditching operation is carried out, the width of a planting compartment surface is 140 cm, and the raised part of the earth surface is leveled; and (5) after leveling, using a seeder to perform film mulching, fertilization and seeding according to a wide row: narrow row ratio of 50cm: 30cm. In this way, waterlogging damage can be relieved, weeds are inhibited, drought resistance and water retention are achieved, the water and fertilizer utilization rate is increased, and finally disaster avoidance and reduction, high yield and high efficiency of rice field oilseed rape are achieved.

The application belongs to the technical field of genetic engineering and relates to TaGoLS10 application of a gene and an encoded protein in regulating drought resistance of plants. TaGoLS10 The gene can positively regulate the drought resistance phenotype of wheat, and the TaGoLS10 protein encoded by the gene can specifically interact with a drought resistance related protein TaGTs, and both of them cooperatively participate in and regulate the response process of wheat to drought stress, thereby enhancing the drought resistance of wheat. The application adopts a technical strategy combining overexpression technology and RNAi technology, and successfully creates new wheat materials with significantly improved or reduced drought resistance.

The application relates to a rice peroxidase gene OsPRX31 or application of a coded protein thereof in regulating drought resistance of rice, and belongs to the technical field of genetic engineering. OsPRX31 The rice peroxidase gene OsLEA3 participates in drought stress response through an ABA signal pathway, can reduce oxidative stress damage, including reducing active oxygen and malondialdehyde accumulation, improving proline content, and up-regulating drought resistance gene expression, and enhances drought resistance. OsPRX31 Experimental verification shows that rice overexpressing the gene has a higher survival rate after drought, improved physiological indexes, reduced accumulation of oxidized substances, and up-regulated drought resistance gene expression; and knocking out the gene leads to grain abortion, and overexpressing the gene leads to normal seed setting. OsPRX31 OsPRX31 The application can effectively improve the drought resistance of rice and maintain fertility, and provides gene resources and theoretical basis for drought resistance molecular breeding.

This invention discloses the application of the wheat gene TaPSS2 in improving plant drought resistance, belonging to the field of functional genomics. The nucleotide sequence of the wheat gene TaPSS2 is shown in SEQ ID NO: 2. Transforming this gene into the model plant Arabidopsis thaliana can significantly improve the drought resistance of Arabidopsis. If the wheat gene TaPSS2 is transformed into wheat, rice, maize, cauliflower, and other plants, it is possible to obtain new germplasm with drought-resistant characteristics, which is of great significance for the breeding of superior crop varieties and their widespread application in production.

This application provides a method for optimizing the tiered drought limit water level during the flood season, taking into account both supply and demand. It obtains the downstream control station of the target river channel, tiered water supply demand, initial tiered outflow parameters, and target tiered outflow parameters. Based on a preset low-water inflow sample and the target tiered outflow parameters, it calculates the initial tiered drought limit water level of the upstream reservoir during the flood season. It determines the drought resistance capacity parameters on the supply side based on historical reservoir operation data, and determines the target tiered drought resistance flow gap parameters on the demand side based on the actual outflow and the target tiered outflow parameters. Simultaneously, it determines the risk value of flood control loss conditions based on the reservoir's flood season operating water level and the river's excess discharge. A scheduling simulation model is constructed to perform multi-objective optimization of the initial tiered drought limit water level during the flood season, resulting in a target tiered drought limit water level that balances both supply and demand. This method is adaptable to complex hydrological scenarios such as flood season reversals and rapid shifts between flood and drought, and can collaboratively ensure downstream tiered water demand, reservoir drought resistance capacity, and flood control safety.

The application discloses OsHAK13 Application of a gene in regulating drought tolerance of rice relates to the technical field of genetic engineering. OsHAK13 The gene encodes an amino acid sequence as shown in SEQ ID NO. 2. OsHAK13 By knocking out the gene through a gene editing technology, a functional loss mutant is obtained, which shows significant enhancement of drought tolerance at a seedling stage. The gene and the mutant can be applied to genetic improvement of crop drought resistance, and provide important gene resources and breeding materials for cultivation of new water-saving and drought-resistant rice varieties, and have important theoretical significance and application value.

The application discloses a PARMS molecular marker significantly related to the tryptophan content of corn root systems and application thereof, the nucleotide sequence of 150 bp upstream and downstream of the molecular marker is shown as SEQ ID NO. 6, and the primer group nucleotide sequence for detecting the PARMS molecular marker is shown as SEQ ID NO. 1-SEQ ID NO. 3. ZmASB1 The application successfully develops a molecular marker based on the natural variation of the 5'UTR region of the (Zm00001eb080510) gene, the marker is significantly related to the tryptophan content of corn root systems, can accurately, efficiently and stably distinguish the InDel + genotype and the InDel ‑ genotype. The molecular marker can be widely applied to the tryptophan content of corn root system germplasm resources and molecular marker assisted breeding, and provides important technical support and theoretical basis for cultivating new corn varieties with high yield, stable yield and drought resistance.