1159 results about "Drought resistance" patented technology

The invention relates to a Chinese wildrye rhizosphere growth-promoting bacterium and application of the Chinese wildrye rhizosphere growth-promoting bacterium in growth promotion and drought resistance of Chinese wildrye, and belongs to the technical field of microorganisms, the strain is classified and named as Paraburkholderia graminis, the strain number is B24, and the preservation number is CGMCC No.31907. The strain provided by the invention is derived from the intergrassland soil of leymus chinensis in an excessive grazing grassland, has good growth promoting characteristics of producing IAA, producing ACC deaminase, fixing nitrogen, dissolving phosphorus and the like, and also can obviously enhance the drought resistance of the leymus chinensis. When the strain is inoculated to the rhizosphere of a leymus chinensis seedling, the growth of the leymus chinensis can be obviously promoted, and the overground and underground biomass of the leymus chinensis is increased; the activity of antioxidant enzymes is reduced and the hormone content in leymus chinensis leaves is changed, so that drought stress is relieved, the photosynthesis of leymus chinensis is enhanced, and the drought resistance of leymus chinensis is remarkably improved. Therefore, the Burkholderia parakholderia can be used for development of a microbial fertilizer for grassland plants and applied to a drought stress habitat, so that the drought resistance of the grassland plant leymus chinensis is improved, and grassland management is optimized.

The invention relates to the technical field of molecular marker development and molecular marker assisted breeding, and particularly discloses a KASP molecular marker closely linked with a wheat drought resistance related gene TaRPL36-8, a primer and application, the nucleotide sequence of the KASP molecular marker is shown as SEQ ID NO.1, and T / C polymorphism exists at the 201bp position of the sequence. By detecting the KASP molecular marker, the drought-resistant variety of wheat is identified, and technical support is provided for wheat breeding.

The invention discloses a comprehensive evaluation method for drought resistance of white birch germplasm resources in the seedling stage, and belongs to the technical field of drought resistance evaluation. Under different gradient drought stress and normal conditions, 14 drought resistance indexes of 90 white birch half-sib families are measured, and the drought resistance is comprehensively evaluated by combining membership function analysis, principal component analysis and clustering analysis. A comprehensive drought resistance evaluation value (D value) is obtained according to a membership function, comprehensive clustering analysis is carried out according to the D value of each family, 90 families are divided into four categories at the Euclidean distance of 1.5, and BWY32 and BWY29 are screened out as drought-resistant families. A stepwise linear regression analysis method is utilized to respectively establish linear regression equations under drought stress 5d and 10d, and four important drought resistance indexes are screened out. Compared with the previous research, the method fully considers the difference of the sensitivity degree of each selected index to drought stress and the contribution rate of each index to drought resistance evaluation, and lays a good foundation for simplification and high efficiency of the drought resistance evaluation of the white birch.

The invention belongs to the technical field of agriculture, and discloses a method for identifying and selecting drought-resistant and salt-tolerant wheat varieties in a growth period, which is used for identifying and selecting the drought-resistant and salt-tolerant wheat varieties in the growth period of different wheat varieties under the stress of salt with soil salt content of 0.3% and 0.6% as a correlation analysis result of membership function values and comprehensive evaluation values of all indexes in the whole growth period of the wheat. The above-ground biomass in the heading stage and the filling stage is preliminarily determined as a key stage for evaluating the drought resistance, and can be used as an identification index for screening salt-tolerant varieties. The salt tolerance comprehensive index established through the indexes can be used for clustering test varieties, a direct and simple basis is provided for variety salt tolerance identification and grading, and the salt tolerance of wheat varieties (lines) can be well evaluated by utilizing a whole-growth-period comprehensive model.

The invention discloses an ecological restoration method for a bare rock side slope in a high and cold arid area, and belongs to the technical field of ecological restoration, the ecological restoration method for the bare rock side slope in the high and cold arid area comprises the following steps: (1) side slope pretreatment: firstly, mechanically chiseling a bare rock slope surface to form micro holes; a rhombic iron gauze is laid and anchored; (2) spray-seeding an anti-freezing and thawing base material to ensure that the base material uniformly covers the slope surface; wherein the freeze-thaw resistant base material comprises the following raw materials: weathered gravels, straw fibers, attapulgite clay, an inorganic compound fertilizer, a slow-release water-retaining agent, a cold-resistant microbial agent and mixed anti-freezing grass seeds; (3) covering the surface of the bare rock slope with an ecological blanket; (4) water management. The structural stability is enhanced through mechanical pretreatment, the anti-freezing and drought-resisting capabilities are improved through the composite anti-freezing and thawing base material, vegetation growth is promoted through cooperation of microorganisms and grass seeds, precise maintenance is achieved through an intelligent system, and a systematic solution for ecological restoration of the bare rock slope in the high and cold arid area is formed. The method has remarkable advantages in the aspects of improving the restoration efficiency, guaranteeing the ecological sustainability and the like.

The invention discloses a gene PtoMYB20 for regulating and controlling salt tolerance and drought resistance of poplars. A nucleotide sequence of the gene comprises a sequence as shown in SEQ ID NO. 1. The gene interference fragment is introduced into a plant to obtain a silent transgenic plant, under the conditions of salt stress, drought stress and salt-drought composite stress, the H2O2 and MDA contents of the silent transgenic plant are lower than those of a wild plant, and the SOD and POD activities of the silent transgenic plant are higher than those of the wild plant, so that the damage degree of the plant is effectively reduced; the method is of great significance in improving salt tolerance and drought resistance of the poplar and relieving damage of adversity stress to forestry yield. The invention also discloses a breeding method of the transgenic poplar with strong salt tolerance and drought resistance, provides a new poplar germplasm which not only improves the stress resistance of the poplar but also does not influence the wood yield, and lays a theoretical and gene resource foundation for the stress-resistant molecular breeding of the poplar.

The invention discloses a winter wheat field soil fertility evaluation data processing method based on comprehensive weight calculation, and relates to the technical field of data processing, and the method comprises the steps: S1, collecting nutrient content and moisture retention data from field soil through a sensor network, performing grouping processing on the acquired nutrient content and moisture retention data by adopting decision node splitting and entropy calculation to obtain a soil fertility layering result; s2, according to a soil fertility layering result, acquiring root depth and leaf moisture content indexes of the winter wheat in different layers of soil from crop monitoring equipment, analyzing association strength of the root depth and leaf moisture content indexes and fertility layering by adopting leaf node classification and feature selection standards, and determining a fertility contribution coefficient; according to the winter wheat field soil fertility evaluation data processing method based on comprehensive weight calculation, efficient resource allocation and stable yield are realized, and the drought resistance and resource utilization efficiency of winter wheat in a drought environment are remarkably improved.

The invention relates to the technical field of mountain afforestation, provides an efficient nursery stock planting method suitable for mountain afforestation, and aims at solving the problems that in existing mountain afforestation, planting is not standard, the survival rate is low, the water and soil conservation capacity is weak, and the labor intensity is high. The forest space layout is optimized, and the water and soil conservation capability is enhanced; the mellow soil and immature soil layered stacking and backfilling technology is adopted, the natural structure of the soil is recovered, and the root zone microenvironment quality is improved; a fertilizer isolation layer is arranged and degradable container seedlings are implanted, so that the risk of root burning is avoided, the integrity of root systems is protected, and the survival rate of the seedlings is remarkably increased; by combining root fixing water slow irrigation, water retention covering and slope water collection and soil conservation structures, natural rainfall is effectively utilized, evaporation and runoff are reduced, and water saving and drought resisting are achieved; the whole process flow is standard, the replicability is high, and the dependence on artificial experience is reduced.

The invention discloses an application of an msa-miR156e gene regulated by NO in regulation of drought resistance of plants, and is characterized in that a mature sequence of the msa-miR156e gene is as shown in SEQ ID No. 1, and a precursor sequence of the msa-miR156e gene is as shown in SEQ ID No. 2. The invention also discloses an application of the msa-miR156e gene in regulation of drought resistance of plants. According to the application, the mechanism of NO for regulating the alfalfa msa-miR156e gene to cope with drought stress is studied, the NO is found to up-regulate the expression of msa-miR156e, enhance the activity of antioxidant enzyme and reduce the accumulation of active oxygen, so that the drought resistance of alfalfa is remarkably improved, SPL family genes are regulated in a targeted manner through msa-miR156e, the physiological adaptability of plants is promoted, and the theoretical basis of plant stress resistance is enriched; the cultivation of a high-quality alfalfa variety adapting to a drought environment is facilitated.

The invention provides bacillus subtilis KC-1 as well as a composition and application thereof, and belongs to the technical field of microorganisms. The preservation number of the bacillus subtilis KC-1 is CGMCC (China General Microbiological Culture Collection Center) No.35237. The bacillus subtilis KC-1 can secrete IAA, has nitrogen fixation capacity and can generate siderophores. The bacillus subtilis KC-1 and whey protein are used as a composition, and the bacillus subtilis KC-1 and the whey protein jointly act to prepare the seed treatment material, so that a synergistic effect can be achieved, and corn seed germination and seedling root development are remarkably promoted. Experiments show that compared with single use of the whey protein and the bacillus subtilis KC-1, the combined use of the whey protein and the bacillus subtilis KC-1 can significantly improve the seed germination rate, the length of buds on the overground part, the length of main roots on the underground part, the total root volume, the total root surface area, the total root length and the seedling auxin concentration, and also can significantly reduce the concentration of abscisic acid in seedlings, so that the survival rate of the seedlings is improved. The germination of seeds and the growth of seedlings under drought conditions are promoted, and the drought resistance of plants is improved.

The invention relates to the technical field of plant genetic engineering, in particular to a gene of leymus secalinus participating in adversity stress and application of the gene. The gene LsHSFA participating in adversity stress in leymus secalinus is found for the first time, the nucleotide sequence of the gene LsHSFA is shown as SEQ ID NO.1, and the amino acid sequence of the coded protein is shown as SEQ ID NO.2. Through cloning and identification of the adversity stress participating gene of the leymus secalinus, expression analysis of the gene, genetic transformation of the gene and other means, the function of the gene is analyzed and verified, and the result shows that the adversity stress participating gene of the leymus secalinus has important influence on promotion of upper development of the leymus secalinus and improvement of heat resistance, salt resistance, drought resistance and other stress resistance; the stress-resistant gene of the leymus secalinus is utilized to positively regulate and control the abiotic stress function, so that the leymus secalinus can cope with various stress adversities better, the leymus secalinus can adapt to more complex and harsh ecological environments, degenerated and desertified grassland is improved, and potential application prospects are provided for improved variety breeding and excellent population construction of the leymus secalinus in the future.

The invention relates to the technical field of plant drought tolerance, in particular to a plant drought tolerance related protein FYVE4 and application of a coding gene thereof. By inhibiting expression or protein activity of the FYVE4 gene in the plant, the survival rate of the plant under drought stress is increased; according to the method, in genetic improvement of arabidopsis thaliana, after genetic transformation is conducted through an agrobacterium-mediated method or a gene gun method, the stomata closing response speed and chlorophyll accumulation amount of crops can be effectively increased, and therefore plants with stable genetic drought resistance characters are established; the technical scheme is particularly suitable for constructing a water-saving agricultural system, has very important significance for improving crops and cultivating drought-resistant crops, and is suitable for popularization and application.

The invention discloses haplotype molecular markers related to wheat drought resistance, primers, a kit and application of the haplotype molecular markers, and aims to solve the technical problem that molecular markers capable of identifying excellent haplotypes of TaXTH-2B gene promoters are lacked at present. It is confirmed that the TaXTH-2B gene promoter has two haplotypes, and the Hap II haplotype is an excellent haplotype endowing wheat with a high drought resistance index and has high promoter activity; meanwhile, the invention develops molecular marker primers for distinguishing the two haplotypes, and the molecular marker primers have wide application prospects in the aspects of identifying and screening bred wheat varieties or breeding intermediate generation materials, assisting in selecting wheat materials with high drought resistance indexes and the like.

The invention belongs to the field of plant genetic engineering, and discloses application of a PtNF-YB9 gene of trifoliate orange in drought-resistant genetic improvement of plants. The PtNF-YB9 gene is a transcription factor gene which is separated and cloned from Poncirus trifoliata, and the sequence of the PtNF-YB9 gene is as shown in SEQ ID NO. 1. The gene or the homologous gene thereof is knocked out or inhibited in a plant, the drought resistance of the plant is remarkably reduced, and the drought resistance of the plant is remarkably enhanced by overexpressing the gene or the homologous gene thereof. The development and utilization of the genetic resource are beneficial to reducing the agricultural production cost and realizing the green and environment-friendly agricultural goal.

The invention discloses application of an OsCPN10a gene in regulation and control of vigor, grain shape and drought tolerance of rice seeds, and belongs to the technical field of rice gene engineering. The invention particularly relates to the research of the OsCPN10a gene in the aspects of regulating and controlling the germination rate, the germination potential, the germination index, the grain length, the grain width, the thousand grain weight, the drought tolerance of the rice seeds and the like. Experimental results show that the grain shape of the OsCPN10a knockout mutant seed is obviously lengthened and widened, and the grain shape of the OsCPN10a overexpression seed is opposite; the yield of the Ocpn10a mutant is higher than that of a TNG67 plant; overexpression of the OsCPN10a has the potential of improving the drought resistance of the rice. The invention reveals that OsCPN10a affects genetic mechanisms such as rice seed vigor, grain length, grain width and storability, provides important gene resources and theoretical basis for genetic improvement of rice, and is beneficial to cultivation of a new rice variety with better grain shape and higher yield.

The invention discloses a rice drought resistance phenotype detection and genetic analysis method based on a phenotypic robot, which is characterized in that rice hyperspectral phenotypic character data is acquired based on the phenotypic robot, and the relative water content RWC of rice is predicted. Through high-frequency data acquisition, RWC (t) based on a time sequence is obtained, RWC (t) curve change characteristic indexes are calculated, the dynamic change process of a rice experiment material in the whole drought experiment period is quantified, the drought resistance index DTI of the rice experiment material is obtained, and the drought resistance of the rice variety is comprehensively evaluated. DTI as a user-defined phenotypic character can be used for whole genome association analysis (GWAS), and a key gene for controlling the drought resistance of rice is excavated. According to the invention, the problems of low efficiency and low flux of traditional rice phenotype detection are solved, phenotype-gene mining based on the mobile robot is realized, and a new way is expanded for improvement of rice genetic breeding.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for identifying drought tolerance of wheat, haplotypes, primers and application. Belongs to the technical field of biological breeding. Research finds that three SNP loci exist on a wheat ARF20-B gene, a wheat drought-enduring haplotype ARF20-BHapI is developed based on the SNP loci, the first locus is located at the 3616bp position of the ARF20-B gene, and the A / G nucleotide polymorphism is A; the second site is located at the 5623bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism; the third site is located at the 9795bp position of the ARF20-B gene, and G is in the G / A nucleotide polymorphism. The wheat carrying the ARF20-BHapI haplotype has the advantage that the drought tolerance of the wheat carrying the ARF20-BHapI haplotype is higher. The invention further provides a KASP primer group for detecting the ARF20-BHapI haplotype, and successful detection of the drought tolerance of the wheat is achieved.

The invention provides a wheat drought-tolerant gene TaTZF1 and application thereof in plant drought tolerance, and belongs to the technical field of gene engineering. The invention provides a wheat drought-enduring gene TaTZF1. The amino acid sequence of a protein encoded by the wheat drought-enduring gene TaTZF1 is as shown in SEQ ID No. 2. The wheat drought-enduring gene TaTZF1 is subjected to induced expression by drought, ABA and salt stress, the wheat drought-enduring gene TaTZF1 is overexpressed and transformed into wheat in an overexpression mode, and it is found that the drought tolerance of a transgenic plant with overexpressed TaTZF1 is remarkably improved compared with that of a wild type plant; after the wheat drought-tolerant gene TaTZF1 in wheat is knocked out, the drought tolerance is obviously reduced. It is proved that the wheat drought-resistant gene TaTZF1 positively regulates the drought resistance of wheat, gene resources are provided for wheat molecular breeding, and the wheat drought-resistant gene TaTZF1 has great significance in improvement of wheat drought-resistant genetic improvement.

The invention provides a soil drought resistance determination method, device and equipment and a storage medium, and the method comprises the following steps: determining a specific drought-susceptibility index of a target soil sample in a specific water content state based on the volume, saturated water content, saturated hydraulic conductivity and a soil water holding curve of the target soil sample; the specific drought-susceptibility index represents the power required for transferring unit water from unit volume of a target soil sample to the surface of a crop root system in unit time; and determining the drought resistance of the target soil sample based on the specific drought-susceptibility index of the target soil sample. Therefore, the drought resistance of the soil is comprehensively, accurately and quantitatively evaluated, and a theoretical basis and practical guidance are provided for comparison and improvement of the drought resistance of the soil in agricultural production.

The invention relates to a crop drought-resistant agent, a method for improving crop drought resistance and / or promoting crop growth and development and application of the crop drought-resistant agent, and belongs to the technical field of agricultural production. The crop drought-resistant agent is prepared from the following components: arginine, chitosan, salicylic acid, diethyl aminoethyl hexanoate, betaine, brassinolide and water. The crop drought-resistant agent can promote normal growth and development of crops under drought conditions, improve plant biomass accumulation and cell antioxidant enzyme activity and reduce toxicity of ROS to the plants, so that adaptability, tolerance and resistance of the crops to drought stress are improved, and the crop drought-resistant agent has the advantages of being good in drought-resistant effect, high in stability and high in safety.

The invention discloses a comprehensive evaluation method for drought resistance of corn varieties, which relates to the technical field of corn variety evaluation, and comprises the following steps: obtaining multi-temporal multi-band reflection spectrum data under the condition of continuous drought of corn, and performing structure coupling modeling on the spectrum data in combination with the structural characteristics of corn leaf epidermis wax, so as to obtain the drought resistance of the corn varieties; constructing a time sequence curve capable of representing the dynamic change of leaf wax distribution as a dynamic spectrum monitoring baseline; differential analysis between continuous time points is carried out based on the dynamic spectrum monitoring base line, spectrum abrupt change points caused by leaf wax migration are identified, and space consistency inspection is combined. According to the method, a dynamic spectrum monitoring baseline and an energy migration fingerprint are constructed, wax migration interference is accurately identified, a spectrum stability hypothesis is broken through, high precision, self-adaption and consistency of evapotranspiration rate inversion under a drought condition are realized through multi-level correction and a closed-loop updating strategy, and scientificity and comparability of drought resistance evaluation are improved.

The invention discloses application of a transcription factor BnaMYB52 in regulation and control of drought resistance of rape, and relates to the technical field of biology. The amino acid sequence of the transcription factor BnaMYB52 is as shown in any one of SEQ ID NO. 5 to 8. According to the invention, the negative regulation effect of the transcription factor BnaMYB52 in the drought resistance of the rape is disclosed for the first time, and a brand new molecular target and a genetic improvement strategy are provided for improving the drought resistance of the rape. Through in-depth study, it is found that knockout of the BnaMYB52 gene can significantly enhance the tolerance of rape plants to the drought environment. In addition, a new drought-resistant rape germplasm obtained after genetic modification of the gene shows excellent adaptive capacity in a drought stress environment. The technical breakthrough not only helps to solve practical problems faced by rape planting in arid regions, but also lays a solid theoretical foundation and technical support for cultivating high-yield and high-quality rape varieties adapting to extreme climate conditions.

The invention discloses application of GmFLZ protein or a coding gene thereof in drought tolerance of plants, in particular to application in drought tolerance of soybeans. The soybean GmFLZ protein or the coding gene thereof provided by the invention has a soybean drought-enduring function. According to the invention, the GmFLZ gene is introduced into soybean roots through an agrobacterium transformation method, so that a GmFLZ gene overexpressed soybean complex plant is obtained, and the drought tolerance of soybeans is remarkably improved.

The invention provides application of an OsRTD1 gene in improving drought resistance of rice, and belongs to the technical field of gene engineering and rice drought resistance. The nucleotide sequence of the OsRTD1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the protein coded by the OsRTD1 gene is as shown in SEQ ID NO: 2. Compared with a wild plant, the survival rate of the OsRTD1 gene mutant plant osrtd1 under the drought stress of PEG induction and soil dehydration is remarkably improved, the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced, the relative water loss rate of dehydrated leaves is higher, and the survival rate of the OsRTD1 gene overexpression plant OsRTD1-OE under the drought stress is remarkably reduced. The total grain number, the solid grain number, the total grain weight and the like of the OsRTD1 gene mutant plant osrtd1 under the stress of field drought are obviously higher than those of a wild plant. The OsRTD1 gene is a potential candidate new target for regulating and controlling a rice drought resistance mechanism.

The invention discloses a corn drought resistance identification device and method, and belongs to the technical field of agricultural scientific research. According to the method, the problems of inaccurate environment control, long identification period, poor repeatability and the like of an existing method are solved, the drought resistance index of the corn is calculated through the random forest model, the drought resistance grade of the corn is divided, and the drought resistance index threshold value is set; the drought resistance indexes of the corn in different growth periods are analyzed, the drought resistance index threshold value is dynamically adjusted according to the corn growth data monitored in real time, and the drought resistance of the corn in different growth periods can be reflected more accurately; feature importance scores are calculated through a random forest model, feature variables are sorted, and it can be determined that certain features make the greatest contribution to the drought resistance index; based on feature importance analysis, suggestions on corn drought resistance research and breeding are provided, researchers can be helped to optimize breeding strategies, errors caused by subjective judgment are reduced, and the breeding efficiency of drought-resistant varieties is improved.

The invention discloses a method for improving drought tolerance and yield of wheat through combined application of compost and sodium selenite, and belongs to the technical field of agricultural planting. According to the method, wheat is planted after compost is applied to soil, and when drought stress occurs, a sodium selenite solution is sprayed to wheat leaves, so that oxidative stress indexes such as MDA, H2O2 and O2 <-> of wheat can be remarkably reduced, and the activity of antioxidant enzymes such as SOD, POD, CAT and APX can be improved; physiological indexes such as chlorophyll a, chlorophyll b, total chlorophyll, carotenoid, protein, soluble sugar and proline of wheat leaves are improved, growth and yield related traits of the wheat are improved, and finally the drought resistance and yield of the wheat are improved. The compost is prepared by taking 50%-55% of corn straw, 10%-20% of flue-cured tobacco straw, 20%-25% of rice straw, 10%-15% of livestock manure and 0.1%-1% of bacillus subtilis as raw materials, adding organic wastewater or tap water, mixing, composting and fermenting.

The invention discloses a method for inducing infiltration of root systems of sandy vegetation. The method comprises the steps of land selection and preparation, pit digging, vegetation selection, arrangement of a PVC pipe water applying system, vegetation planting and backfilling and layered water control operation. According to the method, PVC pipes with water seepage holes with different depths are arranged near the root system of vegetation, a self-descending and lifting piston structure of a water-absorbing expansion cylindrical block and a depth-keeping hook-hanging ring chain is adopted in the pipes for the first time, and the distance between the water seepage holes, the length of a rope section and the size of a hanging ring are accurately calculated, so that it is guaranteed that the distance between the water seepage holes and the rope section is accurately calculated at each stage; water can only seep from the water seepage holes with the specific target depth, the root system is guided to follow a water signal to be deeply pricked downwards continuously, a deep and developed root system is formed, accurate layered water control and root system directional induction are achieved, the drought resistance and wind falling resistance of vegetation are greatly enhanced, the water consumption is low, the vegetation survival rate is high, and the growth vigor is good; the depth-keeping water applying system serves as a multifunctional and permanent ecological restoration unit and further has the functions of soil ventilation, humidification and self-cleaning.

The invention relates to the technical field of plant biology, in particular to a sugarcane gene ScPYL61 as well as application, an overexpression vector and a method thereof. The nucleotide sequence of the sugarcane gene ScPYL61 is as shown in SEQ ID No.1 (Sequence Identifier Number 1). According to the invention, 63 PYL gene family members are totally identified from a sugarcane No.1 variety in a sugarcane drought-resistant cultivated species, and the only one gene ScPYL61 which is high in expression and is induced by PEG and ABA is identified, and the gene activates an ABA signal, promotes stomata closing and root system elongation under drought stress by depending on an ABA-ScPYL61-ScPP2C49-ScSnRK2s molecular module, and enhances the drought resistance of plants. The identification of sugarcane PYL gene family members and the functional analysis of ScPYL61 in the invention lay a theoretical foundation for drought-resistant genetic breeding of sugarcane and provide an effective candidate gene.