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44 results about "Crassulaceae" patented technology

The Crassulaceae, also known as the stonecrop family or the orpine family, are a diverse family of dicotyledon flowering plants characterized by succulent leaves and a unique form of photosynthesis, known as Crassulacean acid metabolism (CAM). Flowers generally have five floral parts. Crassulaceae are usually herbaceous but there are some subshrubs, and relatively few treelike or aquatic plants. Crassulaceae are a medium size monophyletic family in the core eudicots, among the order Saxifragales, whose diversity has made infrafamilial classification very difficult. The family includes approximately 1,400 species and 34–35 genera, depending on the circumscription of the genus Sedum, and distributed over three subfamilies. Members of the Crassulaceae are found worldwide, but mostly in the Northern Hemisphere and southern Africa, typically in dry and/or cold areas where water may be scarce. although a few are aquatic.

Sexual reproductive method of sedum alfredii

InactiveCN101057542APromoting the popularization and application of restoration technologySeed and root treatmentCultivating equipmentsSedum alfrediiDicotyledon
The invention discloses a sexual reproducing method of southeast Crassulaceae, which comprises the following steps: harvesting ripe seed of Crassulaceae; exposing; picking; sieving; separating; airing under shadow; reserving at -20-0 deg. c; freezing the seed below 4 deg. c for 2-3 weeks before germinating; using tap water to select seed; sterilizing the seed through 0. 5%KMnO4; germinating on the paper bed; harvesting the seeding; transmitting the seeding into the base with vermiculite and pearlite; culturing through full-nourishing liquid of dicotyledon; transplanting the seeding into field until the seeding is 3-4cm. The invention can harvest large amount of sexual reproduced sprout of southeast Crassulaceae, which can restore the plant in the heavy metal polluted environment.
Owner:ZHEJIANG UNIV

Method for rapid in-vitro propagation of Crassulaceae plant

The present invention discloses a method for rapid in-vitro propagation of a Crassulaceae plant. The method comprises the steps of carrying out aseptic germination of seeds, inducing adventitious bud, and carrying out in-vitro rooting of the adventitious buds. The method allows in-vitro germinated seedlings to normally grow and to be induced within a short period of time to continuously differentiate from original single-seed single seedlings into single-seed multiple seedlings, so the propagation cycle is greatly shortened, and the growth cycle of the differentiated seedlings is greatly shortened. The method provides a stable technical platform for improvement of the breeding efficiency of high-quality varieties of the Crassulaceae plant.
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI

Health tea of aizoon stonecrop herb

The health tea of aizoon stonecrop herb is made with leaf and tender stem of aizoon stonecrop herb. The making process includes the steps of collecting leaf and tender stem of aizoon stonecrop herb, deactivating enzyme in hot water at 70-90 deg.c, cooling and rinsing, kneading in a kneading machine, stoving at 100-120 deg.c, kneading for the second time, stoving to form, crushing, filtering to eliminate broken slag and hermetically packing. The health tea is scented, delicious and sweet, and has the health functions of improving sleep, lower blood pressure, preventing cardiac and cerebral vascular diseases, etc.
Owner:张学海

Aseptic germination method of Crassulaceae plant seeds

The invention discloses an aseptic germination method of Crassulaceae plant seeds. The method comprises the steps of aseptic germination of sowed seeds, and rooting culture. The method allows the Crassulaceae plant seeds to be aseptically germinated and cultured to form plants, realizes the mass efficient germination of the Crassulaceae plant seeds within a short time, and allows roots to be generated, rooted seedlings to be transplanted to soil, the transplanted seedlings to rapidly grow and commercial plants to be obtained within a short period.
Owner:SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI

Controlled release of water and fertilizer into lawn grass Crassulaceae, Poaceae three-dimensional greening turf

InactiveCN102283088AThree-dimensional greening construction is fastLow cost maintenanceCultivating equipmentsSoilless cultivationPeatNonwoven fabric
The invention relates to a three-dimensional greening turf for soilless cultivation, which consists of four layers from bottom to top: non-woven fabric, substrate layer, perforated cloth and turf. The perforated cloth can be perforated non-woven fabric, perforated burlap or perforated sack, and the aperture on it is 1-10 millimeters. The composition of the substrate layer is selected from a mixture of pastoral soil, humus, sand, perlite, peat soil and microencapsulated fertilizer. During implementation, a layer of non-woven fabric is first laid on the planting bed of the planting site to avoid the substrate layer from being scattered during drafting. The lawn-forming turf of the invention has fast three-dimensional greening construction speed, can promote plant growth, realizes low-cost maintenance, and is a major step towards the selection of three-dimensional greening plants and industrialized seedling cultivation.
Owner:张利民

Method for extraction of Crassulaceae plant cell vacuoles

The invention discloses a method for extraction of Crassulaceae plant cell vacuoles. The method includes protoplast extraction, vacuole coarse extraction, and vacuole purification. The vacuole purification includes: resuspending the vacuoles obtained by coarse extraction in a density gradient medium solution with a mass / volume concentration of 0-5%, adding a density gradient medium solution with a mass / volume concentration of 7-10% into the lower layer of the resuspension solution, and carrying out density gradient centrifugation, thus obtaining purified vacuoles. In the invention, during vacuole purification, firstly the density gradient medium solution with a small concentration is utilized to resuspend the coarsely extracted vacuoles in a centrifuge tube, then the density gradient medium solution with a large concentration is slowly added into the centrifuge tube bottom along the centrifuge tube wall, the solution in the centrifuge tube falls into two layers at the moment, and then density gradient centrifugation is performed. The obtained vacuoles have the advantages of high membrane integrity, large quantity, and high purity.
Owner:ZHEJIANG UNIV

Roof afforestation technology of kalimeris indica

The present invention relates to afforesting technology of building and provides a kind of roof afforesting technology with kalimeris indica as a kind of crassulaceae plant for afforesting various kinds of roofs. The technology includes making frame, planting, stem planting, leaf planting and other steps, and the advantages of light weight, wide application range, low cost and beautifying environment.
Owner:SOUTH CHINA BOTANICAL GARDEN CHINESE ACADEMY OF SCI

Method for cultivating crassulaceae graptoveria gilva

The invention discloses a method for cultivating crassulaceae graptoveria gilva. The method comprises the steps of selecting an environment which is good in sunshine, good in ventilation and dry for leaf cutting, picking leaves of graptoveria gilva, and making the leaves be dehydrated in a dry environment so as to make the leaves in a half wilting state, conducting cutting on the leaves in a cultivating medium to keep the distance between the leaves and the surface layer of the cultivating medium to be 0.5-1 cm, and applying fertilizers every 25-35 days; putting plants of graptoveria gilva obtained after cutting in the sun, and watering the plants once in the morning or evening every 10-15 days. According to the method for cultivating crassulaceae graptoveria gilva, the appropriate cultivating medium for graptoveria gilva is beneficial for cutting, and meanwhile regular watering and fertilizing are emphasized so as to promote the growth of graptoveria gilva; the operation process is simple and convenient, the cost is low, planting and cultivation of graptoveria gilva are facilitated, so that the quality of ornament of graptoveria gilva is improved.
Owner:丽江木悦花卉科技有限公司

Chinese medicine effective component composition for treating liver cancer and method for preparing the same

The invention discloses a traditional Chinese medicine effective part composition capable of treating liver cancer. The composition contains total alkaloids from solanum nigrum extracted from the traditional Chinese medicine Solanum nigrum and salidroside extracted from perennial herb rhodiola root, Rhodiola, Crassulaceae; and the medicine composition has remarkable treatment effect on the liver cancer and has the efficacy of protecting livers. In the effective part composition, the content of the total alkaloids from solanum nigrum of a Solanum nigrum extract is more than 50 percent, and the content of the salidroside in a Rhodiola rosea extract is more than 50 percent. In the medicine composition, the content of the total alkaloids from solanum nigrum is 2 to 6 weight portions more than 50 percent of the Solanum nigrum extract, and the content of the salidroside is 1 to 3 weight portions more than 50 percent of the Rhodiola rosea extract, wherein the preferred proportion is that the content of the total alkaloids from solanum nigrum is 2 weight portions more than 50 percent of the Solanum nigrum extract and the content of the salidroside is 1 weight portion more than 50 percent of the Rhodiola rosea extract. The invention also discloses a method for preparing the medicine composition.
Owner:刘阳

Gadol asexual grafting propagation cultivation technique and gadol exploitation application

InactiveCN101331945ATo achieve the corresponding effectMilk preparationTea substituesSucroseSaccharum
The invention relates a common stonecrop herb agamic grafting reproduction and cultivation technology and common stonecrop herb development and application, belonging to the field of health-care food. The invention technologically grafts the plant common stonecrop herb of the crassulaceae to cynura pinatifida, realizing scientific breeding with 100 percent survival rate of agamic grafting and optimized quality, thus achieving industrialized planting. Aiming at the performance of salidroside and main pharmacology effects, the invention has a rational proportion (divided into 1000 parts based on weight): 100 to 800 parts of common stonecrop herb juice, 0 to 100 parts of ginseng juice, 100 to 200 parts of honey (sugar), 0 to 300 parts of aloe juice, 0 to 120 parts of cynura pinatifida, 0 to 100 parts of angelica, 0 to 300 parts of milk, 0 to 200 parts of fruit juice and 0 to 900 parts of grape wine (fresh beer); the seven produced health-care beverages have good effect and market prospect. In a word, the common stonecrop herb agamic grafting reproduction and cultivation technology and common stonecrop herb development and application obtain substantial results and have basis for deep exploitation and utilization in higher fields.
Owner:丁四泉

Method for promoting lateral bud germination of crassulaceae succulent plants

The invention discloses a method for promoting lateral bud germination of crassulaceae succulent plants. The method comprises the following steps that (1) two years or above crassulaceae succulent plant potted seedlings which are robust in growth and free of diseases and pests are selected, granular organic fertilizers are applied to the surfaces of substrates at February, the application amount of the granular organic fertilizers is 0.2-0.35 kg / m<2> according to the bottom areas of flowerpots, and the potted seedlings are watered thoroughly after fertilization; and (2) after 4 weeks, 60%-70% of leaves at the stems of the plants are removed from bottom to top, 6-BA solutions with the concentration being 800-1200 mg / L are sprayed to the bare stems with a sprayer, the solutions are sprayed once a week and totally sprayed for 4-6 times, the spraying amount of each stem each time is 4-6 mL, and thus the purpose of promoting germination of lateral buds on the stems of the crassulaceae succulent plants is achieved. According to the method for promoting lateral bud germination of the crassulaceae succulent plants, the germination number of the lateral buds on the stems of the plants can be increased to several times of that of blank groups through fertilization, leaf removing, 6-BA solution spraying and other measures.
Owner:WUHAN ACADEMY OF AGRI SCI +1

Crassulaceae plant cultivating and seedling method

The invention belongs to the technical field of plant cultivation, and particularly relates to a crassulaceae plant cultivating and seedling method with an aim to solve the problem that utilization of seeds in breeding and production is difficult in existing crassulaceae plant production. The method includes the steps of seed pretreatment, preparation of breeding plates, seed sowing, greenhouse cultivation, sprouting and the like. By seedling performed with the method, sprouting rate can reach 95%, growing of weeds is avoided, plant diseases are reduced, growth vigor is good, a root system is thriving, spraying of pesticide is not needed, pollution is avoided and the like. With the method, the sprouting rate can reach more than 95% which is more than three times of that with other seed sowing methods, incidence of root rot is less than 0.01%, and purity of crassulaceae plants reaches 100%.
Owner:徐和良

Crassulaceae succulent planting material and preparation method

The invention mainly relates to a crassulaceae succulent planting material and a preparation method. The crassulaceae succulent planting material comprises constituting components, namely, peat soil, rotten leaf soil, coco coir, rice husk, perlite, bone meal, river sand grains, ceramsite, vermiculite, hyuga pelelith, zhijin pelelith and medical stone. By adopting the crassulaceae succulent planting material provided by the invention, plants can grow relatively rapidly. Meanwhile, as the constituting components of the crassulaceae succulent planting material are granules with relatively good water permeability, the phenomena that plant roots are rotted, black rot is caused and the like as the plant roots are soaked in water for a long time can be effectively avoided, and the survival ability of succulent plants can be greatly improved. As the materials of the planting material are natural materials and are subjected to treatment of sterilization and deinsectization, diseases or insects caused by planting materials can be avoided in the growth process of the succulent plants, harmful substances are not retained, and the planting material is a green and environmental-friendly succulent planting material which is applicable to common succulent planters.
Owner:重庆市江津区森德家庭农场

Cultivation method for carpet-like evergreen lawn

The invention discloses a cultivation method for a carpet-like evergreen lawn, which belongs to the technical field of landscaping. Perennial rampant evergreen herbaceous plant stipites of the Crassulaceae are characterized in that roots are grown as soon as the perennial rampant evergreen herbaceous plant stipites of the Crassulaceae touch the ground; the branch tendrils of rampant evergreen grass are planted on a compound substrate without soil or with little soil, and after a few of months, the evergreen lawn which can be rolled and cut for transportation can be cultured. The rolled lawn can be optionally laid on a roof, the ground, a desert or a saline-alkali land to form an evergreen lawn landscape; by means of proper management, the lawn can become an everlasting evergreen lawn; and the cultivation method has the advantages that: the production of the evergreen lawn is programmed, business is commercialized, and all links such as the production, supply, laying and management of the evergreen lawn can be easily standardized.
Owner:胡如意

Crassulaceae root growth-promoting Bacillus aryabhattai and application thereof

The invention discloses crassulaceae root growth-promoting Bacillus aryabhattai JIA2 which has been preserved in the China General Microbiological Culture Collection Center in January 7, 2016, and the preservation No. is CGMCC No. 11967. The crassulaceae root growth-promoting bacillus aryabhattai has very good specificity, can be successfully colonized in root soil of crassulaceae succulent plants, and can remarkably promote growth of the crassulaceae succulent plants.
Owner:SHANDONG FOREST SCI RES INST

Medicinal herbal extract carpediol for treating depression

InactiveUS6905706B2BiocidePowder deliveryHerb medicine1-phenylpropene
The present invention is directed to anti-depressive activity of Crassulaceae special extracts, Carpediol, containing 2-(4-hydroxyphenyl)ethyl-β-d-glucopyranoside or 3-[6-o-(α-1-arabinopyranosyl)-β-d-glucopyranosyl]-oxy-1-phenylpropene in mammals, to method of preparation and analysis of these extract, as well to safety of their usage in combination with other drugs.
Owner:SWEDISH HERBAL INST

Leaf cutting propagation method of succulent plant sedum rubrotinctum

The invention discloses a leaf cutting propagation method of succulent plant sedum rubrotinctum. The method comprises the following steps: picking stock plant leaves of clean sedum rubrotinctum standing in the shade, putting the plant leaves in the shade to dry fractures by air, ensuring that the backs of the leaves are in contact with prepared cultivated soil, culturing the leaves in a room in astigmatism, covering cultivated soil to a root when the root comes out from a leaf, performing foliage fertilization when the quantity of the leaves exceeds 5 and the height of a plant reaches 2cm, and transplanting the plant after the height of the plant is at least 3cm; the prepared cultivated soil is formed by uniformly mixing coarse sand, coco coir and leaf mold, with the same weight, which are dried for 2 to 3 days. The leaf cutting propagation method exclusively belonging to orpine family orpine genus succulent plant sedum rubrotinctum is simple in operation, short in propagation period, high in survival rate and very suitable for large scale farming, and in addition, the adopted cultivated soil adopts coarse sand, coco coir and leaf mold, and on the basis that the culture cost is reduced, the culture effect is promoted and the culture success rate is improved.
Owner:王大吉

Herb of common stonecrop rhizosphere growth-promoting pseudomonas aeruginosa and application thereof

ActiveCN105779365AIncrease the number of germinationIncrease crown widthBiocidePlant growth regulatorsGrowth promotingMicrobiological culture
The invention provides herb of common stonecrop rhizosphere growth-promoting pseudomonas aeruginosa JWP7. The strain is collected in the China General Microbiological Culture Collection Center (CGMCC) on January 7, 2016, with collection number of CGMCC No.11969. The strain has good phosphate solubilizing capacity, can be successfully colonized in rhizosphere soil of crassulaceae succulent plants, can obviously promote growth of root systems and seedlings, subjected to leaf cutting, of the crassulaceae succulent plants, increase the germination number and crown diameter of herb of common stonecrop and increase the shaping rates of the crassulaceae plants and has a broad application prospect.
Owner:SHANDONG FOREST SCI RES INST

Traditional Chinese medicine eye drops for treating pinkeye and preparation method

The invention discloses traditional Chinese medicine eye drops for treating pinkeye and a preparation method, belonging to the field of traditional Chinese medicines. The active ingredients of the traditional Chinese medicine eye drops disclosed by the invention consist of the following raw materials: small fruit fig aerial root, hypericum monogynum, flos buddlejae, Koelreuteria bipinnata Franch., spica prunellae, herba lycopi, trollius chinensis, crassulaceae, griffith phymatopsis, root of Chinese coriaria, fructus sophorae, semen cassiae, pinellia ternata, coffee senna seed and semen sterculiae lychnophorae. The medicinal materials of the traditional Chinese medicine eye drops disclosed by the invention are in proper compatibility and conform to the theories of traditional Chinese medicine and modern medicine; the traditional Chinese medicine eye drops have the functions of diminishing inflammation and easing pain, clearing heat and removing toxicity, clearing heart and improving vision and resisting inflammation and relieving internal heat, and have the advantages of convenience in use, good absorption effect, high effect taking speed, short treatment course and no adverse and toxic and side effects; and according to clinical verification, the traditional Chinese medicine eye drops realize perfect relieving and treatment effects on pinkeye and are suitable to be promoted and applied to clinical treatment and nursing of pinkeye.
Owner:马京平

Method for promoting leaf cutting rooting of multi-fleshy plant of crassulaceae by IBA treatment

The invention provides a method for promoting leaf cutting rooting of multi-fleshy plant of crassulaceae by IBA treatment, and relates to the technical field of plant cutting propagation. The method mainly comprises the following steps: 1, matrix preparation, 2, cutting mother plant selection and leaf treatment, 3, leaf cutting, 4, soil, fertilizer and water management, and 5, seedling transplanting. The leaf cutting treatment step specifically comprises the following substeps: after leaves are picked off, placing the leaves in a cool and ventilated place for shade-drying treatment for 72 h-120 h; starting cutting after wounds at the bases of the leaves are dried and become red, dipping the leaves in IBA with the concentration of 15-20 mg.L<-1> for 1-10 seconds before cutting, wherein thecutting temperature is kept at 25-30 DEG C, the air humidity is 70-80%, and the matrix humidity is 30-35%; and carrying out conventional after rooting. According to the invention, IBA treatment with the concentration of 20mg. L<-1> is adopted after the leaves become red basically or begin to turn red, the formation of root primordia can be effectively promoted through the treatment mode, the rooting rate is improved by 8.88%, the root system effect index is improved by 0.93, and the rooting time is shortened by 5-7 days.
Owner:宿迁市优之雅农业科技发展有限公司

Method for promoting rooting of crassulaceae succulent plant Graptopetalum paraguayense through water planting cuttage

The invention discloses a method for promoting rooting of crassulaceae succulent plant Graptopetalum paraguayense through water planting cuttage. The method mainly comprises the steps: (1) preparing cuttage stem; (2) preparing nutrient solution mother solution; (3) preparing culture solution and performing cuttage fixation; (4) performing nutrient solution management; and (5) performing cuttage stem management. The method is characterized in that a water planting research technology is adopted, branches are increased through seedling cuttage propagation, rooting is promoted through water planting, then nutrient regulation is conducted, and plant leaves are promoted to be colored. By adopting the water planting technology, the nutrient environment conditions of succulent plant growth can bestrictly controlled, the succulent plant root growth is promoted, the cost is low, meanwhile, a root system cannot be damaged, materials are convenient to obtain in the experiment, the dynamic characteristics of root growth are observed, the nutrient absorption capacity of the plant and the regulation level of nutrient elements on plant growth and development are researched, and the accuracy andpertinence of a test result are greatly improved.
Owner:宿迁市博雅农业科技发展有限公司

Butterfly nectariferous plant screening combination

InactiveCN105794730AAnimal husbandryHypolimnasLiliaceae
The invention discloses a butterfly nectariferous plant screening combination. The screening combination comprises a first nectariferous plant combination used for attracting papilionidae, and a second nectariferous plant combination used for attracting nymphalidae, wherein the first nectariferous plant combination comprises a first main genus plant the plant number of which accounts for 60-80% of the total plant number of the first nectariferous plant combination, and the first main genus plant belongs to compositae or rosaceae; the second nectariferous plant combination comprises a second main genus plant the plant number of which accounts for 60-80% of the total plant number of the second nectariferous plant combination, and the second main genus plant is the combination of rosaceae and liliaceae, or the combination of crassulaceae, leguminosae and compositae. The screening combination can attract papilionidae butterflies including sericinus montelus and papilio xuthus as well as nymphalidae butterflies including hypolimnas and melitaea. The screening combination is suitable for being planted in Beijing, a nectariferous plant combination capable of generating an excellent landscape effect can be obtained, the distribution range of butterflies in Beijing is widened, and a new tourism resource, namely flower and butterfly viewing is formed.
Owner:BEIJING FORESTRY UNIV FOREST SCI CO LTD +1

Making method of crassulaceae succulent plant cut flower

The invention relates to a making method of crassulaceae succulent plant cut flower, and belongs to the technical field of plant processing. In the making method, succulent plants in which leaves arenot prone to falling and stems are strong are selected, and after the plants are subjected to soil adhesion, washing, sterilization and airing, the crassulaceae succulent plant cut flower is obtainedby arranging, fixing and binding roots. After later-period maintenance, it can be kept that the cut flower does not fade or excessively grow within 2-3 months, and the ornamental period can reach 4-6months. The succulent cut flower made by using the method is resistant to weak light and long in vase phase, the restriction of lighting to the crassulaceae succulent plants is reduced, the ornamentalvalue of the cut flower is greatly improved, the product can be reused, and the reuse rate reaches 80% or above; besides, the method is simple in operation and low in making cost, the succulent plantapplication field is expanded, the types of cut flowers are enriched, and a foundation is laid for large-scale production of the crassulaceae succulent plant cut flower.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +1

Crassulaceae green-plant system

The invention discloses a crassulaceae green-plant system. The system includes a structural layer, a water-proof layer, a water-discharging layer, a water-preserving layer, a planting layer and a vegetation layer from bottom to top, wherein a crassulaceae plant is planted on the vegetation layer; the planting layer is composed of a fine sand layer, a rough sand layer and a gridding cloth arranged between the fine sand layer and the rough sand layer; plant fibers or / and animal fibers are blended in the fine sand layer; the water-preserving layer is composed of a filtering screen and a sponge, and the filtering screen is arranged above the sponge; two water-discharging plates with through holes are arranged on the water-discharging layer, the through holes in the both ends of water-discharging plates are formed crossly, and the water-discharging plates are arc-shaped and convex upwards; a water-discharging groove is formed in the bottom of each water-discharging plate, a water collecting groove is formed below the both ends of the water-discharging plates and connected with a sprayer through a pump, and the sprayer is positioned above the vegetation layer. The crassulaceae green-plant system ensures nutrients of the vegetation layer and can be achieved recycle of water resources.
Owner:苏州星川园艺绿化有限公司

Green roof structure and construction method thereof

The invention relates to a green roof structure and a construction method thereof. The green roof structure comprises a roof structural layer and further comprises a waterproof heat-preservation layer, a protective isolation layer, a solid water drainage and storage layer, a soil-isolating layer, a soil-isolating baffle, a hilling layer and a plant layer. The plant layer comprises herbaceous plantseeds and crassulaceae plant seedlings. The green roof structure is an energy-saving and environment-friendly system utilizing plants planted on a roof top plate to hinder solar energy so as to prevent a room from overheating. The principle of the green roof structure is that through laying construction of all the layers such as the protective isolation layer, the water drainage and storage layer, the soil-isolating layer, the hilling layer and the plant layer, a heat-insulation, anti-seepage, environment-friendly and energy-saving overall covering roof system is formed. The green roof structure has the characteristics that the construction speed is high, the cost is low, the weight is low, maintenance is hardly needed, and the number of checking times is small.
Owner:THE THIRD CONSTR OF CHINA CONSTR FIRST GROUP +1

Culture medium composition for tissue culture of crassulaceae succulent plants and application of culture medium composition

The invention relates to the technical field of plant tissue culture, and particularly discloses a culture medium composition for tissue culture of crassulaceae succulent plants and application of the culture medium composition. The culture medium composition comprises an induction culture medium, a proliferation culture medium, a seedling strengthening culture medium and a rooting culture medium, wherein the induction culture medium comprises the following raw materials: an MS culture medium, 25-40 g / L of glucose, 0.3-0.8 mg / L of NAA, 2-3 mg / L of 6-BA and 3-5 mg / L of nisin; the proliferation culture medium is prepared from the following raw materials: a B5 culture medium, 35-50g / L of glucose, 0.4-0.6 mg / L of NAA, 1-2mg / L of 6-BA and 40-60 mg / L of sodium hypochlorite; the seedling strengthening culture medium is prepared from the following raw materials: an N6 culture medium, 0.7-0.9 mg / L of NAA, 0.8-1.2 mg / L of 6-BA and 70-90 mg / L of calcium propionate; and the rooting culture medium is prepared from the following raw materials: an MS culture medium, 0.8-0.9 mg / L of NAA and 0.9-1.3 mg / L of 6-BA. The culture medium composition can be used for tissue culture of the crassulaceae succulent plants and has the advantage of quickly performing asexual propagation on the crassulaceae plants.
Owner:龙岩市禾康生物科技有限公司

Tissue culture and propagation method for crassulaceae echeveria agavoides succulent plants

The invention provides a tissue culture and propagation method for crassulaceae echeveria agavoides succulent plants. The tissue culture and propagation method is characterized by comprising the following steps: 1) disinfection treatment of explants, 2) induction culture, 3) subculture, 4) rooting culture, 5) seedling hardening and transplanting to obtain complete plants. According to the method, the propagation survival rate of crassulaceae echeveria agavoides succulent plants can be increased; compared with a traditional propagation mode, the established tissue culture regeneration system is not limited by seasons, materials, and environments, the plants can be produced all the year round by manually controlling the environment needed by the plants, so that the problems that the echeveria agavoides succulent plants are single in propagation mode, low in speed, rare in market and the like are solved, and technical support is provided for industrialization and marketization of seedlings for the echeveria agavoides succulent plants.
Owner:FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI +2
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