247 results about "Dicotyledon" patented technology

The present invention is related to a process based on a source-sink principle, for producing products of interest from crushed or uncrushed germinating dicot seeds comprising an expression system, which is induced or can be induced during germination. The product is either a seed derived composition comprising one or more gene products. Alternatively, it is a product of interest obtained by placing the composition in contact with a substrate, containing a substance capable of being transformed by the seed derived composition as such, dried or in down-stream processed form.

The invention belongs to the technical field of biology and particularly relates to a carrot beta(1,2)xylosetransferase gene full sequence and plasmid construction of CRISPR (clustered regularly interspaced short palindromic repeats) / CAS9 for dicotyledon transfection. The carrot beta(1,2)xylosetransferase gene full sequence is discovered and determined for the first time, two effective gRNAs are designed aiming at the sequence to realize synthesis of corresponding CRISPR / CAS9 plasmids, and thus knockout of beta(1,2)xylosetransferase in suspension carrot cell genomes can be realized. In addition, two STR loci are found in the beta(1,2)xylosetransferase gene sequence, which is remarkably significant to species identification and evolution of carrots.

The invention relates to application of Arabidopsis transcription factor in breeding drought-resistant salt-tolerant rice. Nucleotide sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.1. Encoded protein sequences of the Arabidopsis transcription factor MYB44 are shown in SEQ ID NO.2. Conditions that are met for the nucleotide sequences and the encoded protein sequences include: first, DNA (deoxyribonucleic acid) sequences shown on the 88th site to the 1005th site in the sequence table SEQ ID NO.1, or the sequences highly homologous to the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1; second, other encodable sequencecs with the same protein as the DNA sequences shown in the sequence table SEQ ID NO.2; third, the sequences the same functional as the DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1, or sub-segments contained in the highly homologous DNA sequences shown on the 88th site to the 1005th site in the SEQ ID NO.1. The Arabidopsis transcription factor is applied to breeding of the drought-resistant salt-tolerant rice. An expression vector of the MYB44 gene can be introduced into plant cells by biotechnology. Transforming hosts, available to use the expression vector containing the MYB44 gene, can be monocotyledons such as rice, corn and wheat. The Arabidopsis transcription factor is also applicable to dicotyledons such as tobacco and soybean. The Arabidopsis transcription factor is used to breed drought-resistant salt-tolerant plant varieties.

The invention discloses a special expressing promoter of plant endosperm and appliance, which is characterized by the following: 1) possessing DNA sequence of sequence 1 in sequence table; 2) possessing 70% or above 70% homologous property with limited DNA sequence of sequence 1 in sequence table; possessing DNA sequence with same function; 3) possessing crossing nucleic acid sequence with limited NDA sequence of sequence 1 in sequence table under high strict condition. This promoter can start special expression of external source gene in plant endosperm, which is fit for any plant with endosperm such as monocotyledon or dicotyledon.

The invention relates to a method of enhancing the seed yield and promoting the growth of monocotyledonous or dicotyledonous plants by overexpression of TMT (tonoplast monosaccharide transporter) protein in osogenic or transgenic plant cells. The invention further concerns a transgenic plant having the property of an enhanced seed yield and increased growth as compared with the wild type, comprising a nucleotide sequence which codes for a TMT (tonoplast monosaccharide transporter) protein, as well as a regulatory nucleotide sequence, operably linked therewith, for the control of an increased gene expression of the TMT protein in the plant cells of the transgenic plant. It further relates to the use of the transgenic plant for the cultivation or production of cultivated plants or useful plants, or biomass, oils or proteins produced therefrom.

The invention discloses a special expressing promoter of plant endosperm and appliance, which is characterized by the following: 1) possessing DNA sequence of sequence 1 in sequence table; 2) possessing 70% or above 70% homologous property with limited DNA sequence of sequence 1 in sequence table; possessing DNA sequence with same function; 3) possessing crossing nucleic acid sequence with limited NDA sequence of sequence 1 in sequence table under high strict condition. This promoter can start special expression of external source gene in plant endosperm, which is fit for any plant with endosperm such as monocotyledon or dicotyledon.

The invention relates to an anti-acne cosmetics composition and a facial mask containing the same. The anti-acne cosmetics composition and the facial mask mainly comprise the following components: dicotyledon medicinal cruciferae plant extract, pearl powder, sulfur, calamine, mint, borneol, a surfactant, a adhesive and other additives, have the advantages of high efficiency, safety, enduring efficacy and the like, are simple in preparation process, can effectively realize acne elimination and multiplex sterilization, can radically cure acne in a short time, and can be widely applied in the technical fields of cosmetology and medicine.

The invention discloses a sexual reproducing method of southeast Crassulaceae, which comprises the following steps: harvesting ripe seed of Crassulaceae; exposing; picking; sieving; separating; airing under shadow; reserving at -20-0 deg. c; freezing the seed below 4 deg. c for 2-3 weeks before germinating; using tap water to select seed; sterilizing the seed through 0. 5%KMnO4; germinating on the paper bed; harvesting the seeding; transmitting the seeding into the base with vermiculite and pearlite; culturing through full-nourishing liquid of dicotyledon; transplanting the seeding into field until the seeding is 3-4cm. The invention can harvest large amount of sexual reproduced sprout of southeast Crassulaceae, which can restore the plant in the heavy metal polluted environment.

The invention provides novel methods and compositions for modulating gene function in plants. In particular, the invention provides methods and compositions that allow, for the first time, virus-induced gene silencing in rice. The invention is significant in that prior techniques were not available for rice and because of the major importance of rice to agriculture. The invention therefore provides techniques for the analysis of gene function in rice, as well as in other monocotyledonous species and dicotyledonous plant species.

The invention relates to an alfalfa stress response gene MsNAC3 and an application thereof. A novel NAC (N-Acetyl Cysteine) family related gene is cloned and authenticated from an alfalfa genome by using an RT-PCR (Reverse Transcription-Polymerase Chain Reaction) technology and is named as MsNAC3 with a nucleotide sequence as shown in SEQ ID No.11, and a fusion expression vector containing an enhanced green fluorescent protein (EGFP) gene is constructed. The expression pattern of the gene MsNAC3 in alfalfa and the relation between the gene and adversity stress (cold damage, salt damage and drought) are analyzed by using a fluorescent quantitative PCR technology, in such a way, people find that the cold resistance, drought resistance and salt resistance of a transgenic tobacco plant can be improved through the overexpression of the gene in tobacco; the gene can be used for genetically transforming other monocotyledons and dicotyledons and improving the stress resistance of the monocotyledons and the dicotyledons.

The invention discloses a soybean zinc finger protein gene STF-2 which is a C2H2 type zinc finger protein gene related to drought and newly discovered in soybeans. Experiments show that: the STF-2 encoded protein can be located in the nucleus; after over-expression, the gene can obviously improve the drought resistance of the transgenic plants, and has a function of improving the comprehensive stress resistance of the plants; the gene disclosed by the invention comes from soybeans and has an optimized codon suitable for the dicotyledons such as soybeans and the like, and the genetic engineering receptor thereof is mainly suitable for the dicotyledons such as soybeans, tobaccos, cotton and the like; and moreover, the gene is also suitable for the monocotyledons such as rice, wheat, corn and the like.

The invention discloses an oidium heveae Steinmann in-vivo promoter WY51 and application thereof. The promoter WY51 has a nucleotide sequence as shown in SEQ ID NO: 1, or is provided with a variant with functions of the promoter. The invention further relates to a nucleic acid builder, a carrier, reconstitution cells, a transgenic plant, an explant and a callus which comprises the promoter. The promoter can be used for regulating and controlling expression of exogenous genes in dicotyledon and monocotyledon, and a brand-new tool and selection are provided for expression of genes of the transgenic plant.

The invention discloses a soybean zinc finger protein gene SCTF-1 which is a C2H2-type zinc finger protein gene which is discovered in soybean and is relevant with low temperature. The experiment shows that the protein coded by SCTF-1 can be located to a cell nucleus; after being excessively expressed, the gene can obviously improve cold resistance of transgenic plants, and has a function of improving plant comprehensive stress tolerance. The gene provided by the invention is derived from soybean, and comprises an excellent codon of dicotyledon, such as soybean and the like; and the gene engineering receptor of the gene is mainly suitable for dicotyledon, such as soybean, tobacco and cotton and the like, and also suitable for monocotyledon, such as rice, wheat and corn and the like.

This invention relates to a method for the introduction of genes encoding desirable traits into both monocotyledonous and dicotyledonous plants and to plants and parts thereof produced by growing plants using this method. The time required for the production of transgenic plants is significantly decreased, while the number of transgenic plants is significantly increased. These increases are not dependent upon the use of super-virulent Agrobacterium strains. The invention also relates to an improved technique for in vitro regeneration of mono- and di-cotyledonous plants in a suitable medium containing a novel growth regulator regime that promotes cell elongation in the production of numerous somatic embryos that are regenerable into fertile plants.

The invention discloses a parthenogenesis haploid induced gene DMP and application thereof. According to the invention, parthenogenesis haploid induced genes AtDMP8 and AtDMP9 are cloned from arabidopsis thaliana. Experiments prove that mutation of the AtDMP8 and the AtDMP9 can generate parthenogenesis haploid inducibility, so that the application of parthenogenesis induced haploid extends to dicotyledonous crops. Verification is further carried out in tomatoes, and the invention also finds that mutation of SlDMP can generate parthenogenesis haploid inducibility in tomatoes. The invention laysan important foundation for widening the application of haploid breeding technology in dicotyledons and revealing the biological mechanism of parthenogenetic haploid production. In view of the universality of haploid breeding technology utilization in the current breeding industry, the DMP has very wide application space and market prospect.

The invention discloses a method for cultivating anti-cyanosis transgenic cotton and a special expression vector thereof. The method for cultivating the anti-cyanosis transgenic cotton comprises the following steps: introducing the protein-coding genes of an SPGAFP1 protein into a target plant to obtain a transgenic plant with the cyanosis resistance higher than that of the target plant, wherein the amino acid sequence of the SPGAFP1 protein is sequence 2 in a sequence table; the sequence of the protein-coding genes of the SPGAFP1 protein can be sequence 1 in the sequence table; and the target plant and the transgenic plant are dicotyledon, i.e. cotton, preferably the CCRI 24. Experiments prove that the SPGAFP1 cotton obtained by introducing the expression vector into the cotton can effectively resist fungi initiating cyanosis, thus indicating that the existence of signal peptide greatly enhances the disease resistance of the transgenic plant.

The present invention discloses that, formic acid or salt thereof, is an effective pre-emergent and post-emergent herbicide. Formic acid biodegrades to carbon dioxide and water thus posing not threat the environment. With proper formulation and the use of respiratory protection, formic acid also poses no threat to the applicator. Formic acid is demonstrated in this invention to control both monocotyledonous and dicotyledonous plants.

The invention discloses an efficient cleft grafting method of dicotyledon seedlings and young branches. Rootstocks with 1-2 true leaves (only 2 cotyledons are reserved) or rootstocks with 4-6 true leaves (2 cotyledons and 1-2 true leaves are reserved) are adopted, and stems and the lower edges of petioles are vertically split in the middle along the central axes of the stems from the lower edges of the petioles of the cotyledons and the true leaves; the incisions of scions are cut into a V shape; the stems of the scions are perpendicularly inserted into the incisions of the rootstocks. The incisions of the rootstocks run through the lower sections of the petioles and the central axes of the stems at the same time, so that fixation of the scions is convenient, the scions are closely combined with the rootstocks naturally, nutrient substances produced by photosynthesis of the leaves are transported into the scions nearby through the petioles, and rapid healing of connectors is promoted. In combination with water retention and heat preservation in a recovery period, the survival rate of grafting can be stabilized at 98% or above, and the survival rate of grafting can reach 100% in an appropriate cooperation period of the rootstocks and the scions; according to the method, operation is easy and convenient, grafting is fast, and the survival rate is high.

The invention provides a bionic microfluidic cell culture chip. The bionic microfluidic cell culture chip is based on the moisture conveying characteristic in a dicotyledon reticular vein, and comprises a main channel (6) and a cell culture cavity (9) located in the main channel. The cell culture cavity (9) is isolated from the main channel through a micro-column array (8) with the shape consistent with the shape of the culture cavity. The width of slits of the micro-column array (8) is determined by the size of cultured cells. Cell culture fluid is conveyed to the cell culture cavity through the slits of the micro-column array (8) in a convection diffusion mode, direct impact of the cell culture fluid on the surfaces of cells is avoided, influences of the cell culture fluid on cells are reduced, a stable microenvironment is provided for cell culture in vitro, and cell research accuracy is ensured.

The invention discloses a novel beta-1,6-glucanase, an encoding gene thereof, and applications of the encoding gene. The invention provides the beta-1,6-glucanase gene belonging to the glucoside hydrolase system, wherein the nucleotide sequence of the beta-1,6-glucanase gene is shown in SEQ ID NO.1, and the protein (amino acid) sequence of the encoded outer membrane glucoside hydrolase is shown in SEQ ID NO.2. The beta-1,6-glucanase can effectively prevent the infection of plant pathogenic fungi to plants. An engineered strain established by the gene realizes the prokaryotic expression of the beta-1,6-glucanase gene, and verifies the function of the glucoside hydrolase of the beta-1,6-glucanase gene, the result shows that GluM can effectively inhibit the germination of rice blast spores, and decompose the rice blast spores. After the beta-1,6-glucanase gene is transplanted into the dicotyledonous model plant arabidopsis thaliana and the monocotyledonous model plant rice, the result shows that the obtained transgenic plants have good anti-infection effects for grey mould and rice blast.

The invention was involved in gene engineering field. The method was about using RNA construction region of FCA gene to control blooming to modify crop economic characteristic. Encoded sequences of one or several whole construction region in proteins encoded by FCA whole gene were transferred into target plants. RRM2 construction region covered by rFCA-1 whole gene was amplified by RT-PCR method and was inserted into expression vector pB-1 / 520, then it was transferred into iaponica rice breed medium flower 11.T0 plant with modified gene and T1 (filial generation) showed obvious change on plant shape, leaf shape, size and weight of seeds. The method was applied in economic characteristic of both dicotyledon and monocotyledon.

The invention discloses application of paddy rice BG1 proteins and encoding genes of the paddy rice BG1 proteins to adjusting growth and development of plants and particularly provides application of proteins consisting of the amino acid sequences represented by Sequence 2 in a sequence table or the encoding genes of the proteins to adjusting and controlling growth and development of plants. Experimental results show that overexpression of the BG1 genes in wild Nipponbare causes increase of the sizes of paddy rice grains, the thousand seed weight, the single-plant yield and biomass, and imply the possible application potential of the BG1 genes in agricultural production. Meanwhile, overexpression of the BG1 genes in arabidopsis can enlarge organs (seeds and leaves), which shows that the BG1 genes probably have a common conservative mechanism in adjusting and controlling the sizes of grains (seeds) in monocotyledons and dicotyledons. The BG1 proteins and the encoding genes have a wide market and application prospect in the fields of crop genetic improvement and the like.

The invention discloses an anti-herbicide and anti-pest fusion gene, an encoded protein thereof, and an application of the encoded protein. The fusion gene is formed by an anti-herbicide protein encoding gene and an anti-pest fusion protein encoding gene. The anti-herbicide and anti-pest fusion protein simultaneously has anti-herbicide and anti-pest characteristics; and the quantity of anti-herbicide protein molecules in plants is same to the quantity of anti-pest protein molecules in the plants in the production process of transgenic crops, so the encoded protein can be applied to anti-herbicide and anti-pest fields of monocotyledons and dicotyledon, and is mainly applied to anti-herbicide and anti-pest corn, rice, soybean, wheat and rape.

The invention relates to a promoter derived from powdery mildew of rubber trees and application of the promoter. The invention provides a novel promoter derived from powdery mildew of rubber trees, and the promoter has a nucleotide sequence as shown in SEQ ID NO:1, or a variant with functions of the promoter. The invention further relates to a nucleic acid structure, a vector, a recombinant cell,a transgenic plant, explants and calluses with the promoter. The invention further relates to application of the promoter to regulation and control of target gene expression in fungi. The promoter canbe used for regulating and controlling expression of exogenous target genes in dicotyledon and monocotyledon, and a completely novel tool and option are provided for gene expression of transgenic plants.

The invention discloses a plant resistance associated protein and encoding gene TaERECTA and application thereof. The protein in the invention is selected from the following (a) or (b): (a) protein consisting of amino acids expressed by sequence 2 in a sequence table; and (b) protein derived from (a) related with plant resistance after substitution and / or deficiency and / or adding of one or several amino acid residues of the amino acid sequences of sequence 2 in the sequence table. Experiments prove that the genes in the invention are introduced in the plant so that the resistance of plants can be improved such as drought resistance and / or salt resistance and / or low temperature resistance. Moreover, the genes in the invention are applied to both monocotyledon and dicotyledon. Therefore, the genes in the invention and the application thereof have important significance for cultivating new types of drought-resistant water-saving, salt-resistant or low temperature-resistant crops, thereby being applied to popularization and application.

The invention discloses an endogenous promoter WY7 of powdery mildew of a rubber tree and application of the endogenous promoter WY7. The promoter WY7 has a nucleotide sequence as shown in the SEQ IDNO:1, or the promoter has a variant having the function of the promoter. The invention further relates to a nucleic acid construction body comprising the promoter, a carrier, a reconstitution cell, atransgenic plant, an explant and a callus. The promoter is used for regulating the expression of exogenous target genes in the dicotyledon and the monocotyledon, and provides a brand new tool and choice for the expression of genes of the transgenic plant.

The invention provides a new compound, namely N,N-diethyl-2-(2-methoxy-5-nitrophenoxyl)-ethylamine. The N,N-diethyl-2-(2-methoxy-5-nitrophenoxyl)-ethylamine has an amino ether skeleton and can regulate the growth of plants. The compound is used for performing biological activity experiment on monocotyledon wheat, dicotyledon mung beans and peanuts. The experiment result indicates that for the mungbeans, the peanuts and the wheat, the compound has a remarkable acceleration effect when the concentration is low and has a certain inhibition effect when the concentration is high.

The invention discloses an embedded RbcS cTP gene and an expression vector and application thereof. The gene is formed in the mode that the N-end of arabidopsis RbcS cTP is inserted between the N-end of corn RbcS cTP and an intermediate structure domain, and the cDNA sequence is shown as SEQ ID NO.2. The cDNA sequence of a corn RbcS cTP gene is shown as SEQ ID NO.1. The embedded RbcS cTP gene can be applied when exogenous genes are transferred into chloroplast of monocotyledon and / or dicotyledon.