55 results about "Dicotyledon" patented technology

The invention discloses application of a gene CsPOD7 in cucumber haploid breeding, and belongs to the technical field of molecular breeding. According to the invention, a parthenogenesis haploid induction gene CsPOD7 is cloned from cucumber, the CsPOD7 is knocked out through a CRISPR-Cas9 gene editing technology, and a cspod7 mutant is used as a male parent to be hybridized with other cucumber materials as a female parent, so that parthenogenesis haploid can be generated. The invention is the first application of the gene CsPOD7 in developing a parthenogenesis haploid induction line in dicotyledon, realizes the application of the haploid induction line based on the gene CsPOD7 in cucumber genetic breeding, accelerates the cucumber breeding process, and has huge potential application value and market prospect.

The invention relates to a haploid induction method based on a brassica napus phospholipase gene BnaPLA2-a and application of the haploid induction method, the BnaPLA2-a gene in brassica napus is knocked out through a gene editing technology, a mutant material capable of inducing haploid generation is obtained, and the application blank of a phospholipase pathway in dicotyledon haploid induction is filled. Compared with a traditional microspore culture technology, the invention provides a brand-new haploid induction path which is derived from the rape and is used for the rape. The invention provides a brand new technical tool and germplasm resources for genetic breeding of brassica napus. Meanwhile, the invention discloses a gene, a mutant, a creation method and application in breeding.

Disclosed herein are methods for rapid transformation, genome editing, and regeneration of dicot plants. The methods disclosed herein involve transforming plant cells and explants with recombinant DNA to achieve genome edits. The methods enhance transformation and regeneration efficiency and reduce the time to produce transgenic plants.

The invention relates to the technical field of plant breeding, in particular to a method for constructing a dominant male sterile line based on CIF1 gene overexpression. The brand-new dominant male sterility gene CIF1 is found, it is verified that the gene has functions in monocotyledonous and dicotyledonous plants, and dominant male sterility resources are enriched; besides, a method capable of manually controlling the fertility recovery of the dominant sterile line is found, a reproductive breeding line for large-scale propagation is obtained, male sterile filial generations can be obtained by hybridizing the breeding line with normal plants, and a new application way is provided for large-scale application of dominant sterility.

The invention discloses a tissue-specific promoter screening vector and application thereof, and relates to the technical field of plant genetic engineering. The screening vector comprises an expression vector and a modified GUS (glucuronidase) reporter gene connected to the expression vector, the modified GUS reporter gene is Cat1muGUS, and the sequence of the Cat1muGUS is shown as SEQ ID NO. 3. According to the present invention, the Cat1muGUS variant is developed based on the Cat1GUS, the Cat1muGUS sensitivity is high, the problem of low dyeing screening efficiency caused by the fact that the Cat1GUS is not sheared in the dicotyledonous plant and the monocotyledonous special tissue can be overcome, in addition, the tissue-specific promoter screening vector is constructed based on the Cat1muGUS variant, and the screening efficiency is high; the method can be well applied to screening of all plant and all tissue specific promoters, and the screening efficiency is improved.

Provided are a prime editing system-based method for achieving inversion editing of a large genomic fragment and a composition used. By means of the efficient multi-gene prime editing protein construct ePPEplus in plants, the inversion of large fragments in plants is achieved by means of designing paired pegRNAs (pegL and pegR), and then by means of processes such as reverse transcription, DNA double-stranded annealing, DNA strand synthesis, and repair. The pegL comprises RTT-L, and the pegR comprises RTT-R. The nucleotide sequence of RTT-L is identical to that of strand A at the right terminus of a double-stranded DNA to be inverted. The nucleotide sequence of RTT-R is identical to that of strand B at the left terminus of said DNA. By means of testing in the monocotyledonous plant wheat and dicotyledonous plants Nicotiana benthamiana and tomato, it is indicated that the method is an effective method for mediating precise inversion of large-fragment DNA in plants.

The present application relates to a promoter pAtEF1 alpha for improving gene editing efficiency of dicotyledon plants and application thereof, and belongs to the field of plant genetic engineering.The pAtEF1 alpha promoter can be used for expression of adenine base editing protein, cytosine base editing protein, Cas nuclease and gRNA.The present application further provides an expression cassette group containing the pAtEF1 alpha promoter and a recombinant vector containing the expression cassette group.The pAtEF1 alpha promoter can be widely applied to the fields of gene editing and base editing of dicotyledon plants, and has wide application in genetic improvement of dicotyledon crops such as tomato and biosynthesis of high-value metabolites.

The invention belongs to the technical field of plant biology, and particularly relates to a protein coded by a larch LarGRF1 gene and application of the protein to plant growth promotion. The invention aims to provide a new choice for plant genetic breeding by exploring growth regulatory factors (GRFs) of larch. The technical scheme of the invention is that the protein coded by the larch LarGRF1 gene has an amino acid sequence as shown in SEQ ID No. 2. Through overexpression of the MarGRF1 gene in dicotyledonous plants and monocotyledonous plants, it is found that an overexpression vector can promote proliferation of plant cells, especially proliferation of calluses and accelerate regeneration and growth.

The present invention relates to a composition for enhancing the flavonoid content of a plant comprising a furan-based compound as an active ingredient; a method for producing a plant with enhanced flavonoid content; a plant with enhanced flavonoid content; and a food composition, a feed composition, and an antioxidant composition containing a plant with enhanced flavonoid content, an extract thereof, or a fraction thereof. The furan-based compound according to the present invention activates the biosynthesis of flavonoids, which are representative functional secondary metabolites of plants, thereby inducing the accumulation of functional flavonoids in crops ranging from monocots to dicots, and thereby efficiently enhancing the added value of plants.

The invention belongs to the technical field of gene engineering, and discloses application of a Camellia oleifera CoSPX-MFS3 gene in improvement of phosphorus absorption of plants, and the nucleotide sequence of the Camellia oleifera CoSPX-MFS3 gene is as shown in SEQ ID NO.1. The invention further discloses a preparation method of the Camellia oleifera CoSPX-MFS3 gene. The plant is any one of dicotyledonous plants or monocotyledonous plants. The dicotyledonous plant is any one of arabidopsis thaliana, tobacco or tomato; the monocotyledonous plant is any one of rice, wheat or corn. The low-phosphorus stress can induce the expression of the gene, and the abundance in roots and fruits is the highest. The CoSPX-MFS3 is constructed to an overexpression vector pBI121, and the overexpression vector pBI121 is converted into the silver gray poplar, so that a positive strain is obtained. The invention discloses the application of the Camellia oleifera CoSPX-MFS3 gene in improvement of phosphorus absorption of the plant for the first time, and experiments carried out in the Populus arvensis show that the Populus arvensis plant over-expressed with the CoSPX-MFS3 gene can significantly improve the phosphorus absorption capacity of the plant.

The invention relates to an organic herbicidal composition based on extracts of waste plant matter from the leaves of plants of the Asparagales order, with added amino acids that inhibit the enzyme responsible for glutamine synthesis, which is effective in controlling and eradicating dicotyledon weeds (broadleaf) and monocotyledon weeds (grasses).

The application discloses a pyrazole hexacyclic-5-formamide compound containing an aromatic (ethyl) amide group and a preparation and application thereof. A structural general formula of the compound is shown as formula I. The compound is prepared through an amide condensation reaction, and the preparation method is simple. The pyrazole hexacyclic-5-formamide compound containing the aromatic (ethyl) amide group has an obvious inhibiting effect on growth and development of agricultural pests and growth and development of monocotyledonous or dicotyledonous plants. After application, the pests show symptoms of individual atrophy, skin wrinkling, abnormal molting of insect body deformity and even death, and the plants show that the growth of roots and stems is inhibited. The formula I compound can be applied in agriculture as an insecticide and / or herbicide.

This invention discloses a gene for inducing parthenogenesis in dicotyledonous plants. ZbRWP Its applications, specifically, the parthenogenesis-inducing gene in dicotyledonous plants. ZbRWP This invention is the first to discover and verify its application in cultivating haploid or haploid induction lines of dicotyledonous plants or improving haploid induction ability. RWP This gene family possesses parthenogenetic haploid induction function in dicotyledonous plants, filling a gap in the list of parthenogenetic haploid induction genes in dicotyledonous plants. This invention lays an important foundation for broadening the application of haploid breeding technology in dicotyledonous plants and elucidating the biological mechanism of parthenogenetic haploid formation. Given the widespread use of haploid breeding technology in the current breeding industry, this invention has broad application prospects in the field of bio-breeding.

The application discloses a dicotyledon parthenogenesis induction gene BoRWP and application thereof, in particular a dicotyledon parthenogenesis induction gene BoRWP application in cultivating dicotyledon haploid or haploid induction line or improving haploid induction capacity, the application firstly discovers and verifies that RWP a gene family has parthenogenesis haploid induction function in dicotyledon, and fills the blank of dicotyledon parthenogenesis haploid induction gene. The application lays an important foundation for widening application of haploid breeding technology on dicotyledon and revealing biological mechanism of parthenogenesis haploid generation. In view of the wide use of haploid breeding technology in the current breeding industry, the application has wide application space and prospect in the field of biological breeding.

The present invention relates to a process for extracting xyloglucan from the primary cell walls of plant cells, and more particularly from dicotyledonous plants. The process according to the present invention comprises, in particular, a relatively limited number of steps and uses chemical compounds with low human and environmental toxicity. Figure 1

To provide a biodegradable vegetation sheet that allows for the introduction of dicotyledonous plants and is suitable for the growth of dicotyledonous plants. [Solution] A biodegradable vegetation sheet comprising a biodegradable nonwoven fabric body 11 and a biodegradable reinforcing net 15 attached to the surface of the nonwoven fabric body 11, wherein plant seeds 12 and fertilizer 13 are attached to the back surface of the nonwoven fabric body 11, and the nonwoven fabric body 11, which is formed into a sheet by intertwining fibers, is mainly made of animal hair fibers with keratin as the main component, and the animal hair fibers are intertwined with each other by a needle punching method to form the nonwoven fabric body 11 into a sheet, which has slope erosion prevention effect and fertilizer effect, while enabling the germination of plant seeds.

The invention relates to a method for extracting xyloglucan contained in a primary wall of a plant cell, and more particularly, the plant is a dicotyledonous plant. In particular, the method of the present invention comprises a relatively limited number of steps and uses compounds that are low toxic to humans and the environment.

The application discloses a Gliocladium catenatum PE-1, which is classified as Gliocladium catenatum Clonostachys rosea f. catenulata Clonostachys rosea f , is preserved in the China General Microbiological Culture Collection Center on February 2, 2024, and has a preservation number of CGMCC NO. 41164. The application also discloses a bacterial agent prepared from the Gliocladium catenatum PE-1. The application further discloses applications of the Gliocladium catenatum PE-1 and the bacterial agent in preventing and treating plant diseases. The Gliocladium catenatum PE-1 screened in the application can widely induce the burst of active oxygen of a plant immune marker in monocotyledonous plants and dicotyledonous plants, and can effectively prevent and treat various plant diseases, such as soybean phytophthora root rot, rice rice blast, wheat powdery mildew and wheat scab, and provides a new strain resource and technical support for biological control of plant diseases.

The invention discloses an endogenous promoter AMGT1P11 of mangrove plant avicennia marina and application of the endogenous promoter AMGT1P11, and belongs to the technical field of gene engineering, and the nucleotide sequence of the endogenous promoter AMGT1P11 comprises a sequence as shown in SEQ ID NO: 1, a sequence as shown in SEQ ID NO: 2, a sequence as shown in SEQ ID NO: 3 and a sequence as shown in SEQ ID NO: 4; or a sequence which has at least 70% homology with the sequence as shown in SEQ ID NO: 1 and has the same function as the sequence as shown in SEQ ID NO: 1; or, one or more basic groups are added, substituted, inserted or deleted in the sequence shown in SEQ ID NO: 1, and the sequence has the same function as the sequence shown in SEQ ID NO: 1. The AMGT1P11 contains a reported salt-induced cis-acting element GT1-motif (GGTTAA, a new reference can be provided for research of a mangrove plant salt-tolerant mechanism, meanwhile, the promoter can regulate and control expression of exogenous target genes in dicotyledons and monocotyledons, a brand new tool and selection are provided for plant transgenic engineering, and an important foundation is laid for plant genetic improvement.

The invention provides brassinosterols and application of an inhibitor of the brassinosterols in regulating and controlling the de novo regeneration of dicotyledonous plant root organs, and belongs to the technical field of plant growth regulation and control. According to the invention, brassinosteroid (BR) related mutants are used as materials, and the function of BR in the de novo regeneration process of roots is explored through an in-vitro leaf root regeneration induction method. The result shows that the BR signal can inhibit the root regeneration process, based on this, the invention provides the application of the brassinosteroid synthesis inhibitor in promoting generation of root organs of dicotyledonous plant in-vitro tissue and the application in dicotyledonous plant in-vitro culture, the brassinosteroid synthesis inhibitor is exogenously added, and the yield of the brassinosteroid synthesis inhibitor is improved. The rooting process of the dicotyledonous plant in-vitro leaves can be promoted, the in-vitro culture process is accelerated, and the application value is high.

The application discloses a gene CsPOD7 The application belongs to the technical field of molecular breeding and is applied to haploid breeding of cucumber. CsPOD7 The application clones a parthenogenic haploid induction gene CsPOD7 from cucumber, knocks out the gene cspod7 by CRISPR-Cas9 gene editing technology, and crosses the mutant as a male parent with other cucumber materials as female parents to generate parthenogenic haploids. CsPOD7 The application is the first application of developing a parthenogenic haploid induction line in dicotyledonous plants, realizes the application of the haploid induction line based on the gene CsPOD7 CsPOD7 CsPOD7 CsPOD7 CsPOD7 CsPOD7 CsPOD7 CsPO in cucumber genetic breeding, accelerates the breeding process of cucumber, and has great potential application value and market prospect.

The invention discloses a strain of pyrethrum delavayi PE-1, the classification name of the pyrethrum delavayi PE-1 is pyrethrum delavayi, the pyrethrum delavayi PE-1 is pyrethrum delavayi, the pyrethrum delavayi is preserved in China General Microbiological Culture Collection Center (CGMCC), the preservation date is February 2, 2024, and the preservation number is CGMCC NO.41164. The pyrethrum delavayi has the advantages that the pyrethrum delavayi can be used for preparing the pyrethrum delavayi; the invention also discloses a microbial inoculum prepared from the strain. The invention further discloses application of the bacillus subtilis and a microbial agent prepared from the bacillus subtilis to prevention and treatment of plant diseases. The fermentation liquor of the screened euphorbia emerald PE-1 can widely induce the burst of reactive oxygen of plant immune markers in monocotyledonous plants and dicotyledonous plants, and can effectively prevent and treat various plant diseases such as phytophthora sojae root rot, rice blast, wheat powdery mildew, wheat scab and the like; new strain resources and technical support are provided for biological control of plant diseases.

The application provides a Lemna australis endogenous strong promoter, a recombinant vector containing the Lemna australis endogenous strong promoter, and application of the Lemna australis endogenous strong promoter and the recombinant vector in starting expression of a target gene in monocotyledonous plants and dicotyledonous plants. The Lemna australis endogenous strong promoter provided by the application has high activity in monocotyledonous plants and dicotyledonous plants, can maintain high activity under conditions of different concentrations of screening agents, and basically does not affect expression of adjacent genes under conditions of different concentrations of screening agents. The application can solve problems of the 35S promoter widely used in the prior art, such as difficulty in further improving activity, low activity in monocotyledonous plants, and influence on expression of adjacent genes.

The invention discloses a gene promoter active fragment screening method based on a cross-overlap method and application, and belongs to the technical field of biology. The screening method comprises the following steps: segmenting a target gene promoter sequence, and setting an overlapping fragment between two adjacent segments to obtain a gene promoter active fragment containing the overlapping fragment in the segments. According to the invention, the active fragment of the pennisetum americanum PgGNM2 promoter is screened for the first time, the fragment is used for a CRISPR / Cas9 system, so that the gene editing efficiency can be improved by more than 40%, and when the fragment is used for a yeast one-hybrid bait carrier, the false positive rate can be reduced to less than 5%. The screening method retains the integrity of cis-acting elements, is suitable for monocotyledonous / dicotyledonous plants, and provides an efficient tool for molecular breeding of crops.

This disclosure concerns the use of endogenous plant RNAi machinery to preferentially or specifically reduce transgene expression. In some embodiments, the disclosure concerns specific reduction of transgene expression in seed tissues of a dicot plant.

This disclosure relates to methods of transformation of dicots, such as tomato and watermelon. In some aspects, the methods comprise use of dicot explants obtained by dividing seeds and soaking the dicot explants in various media. In some aspects, transformed cells of a dicot plant are provided as well as dicot plants comprising transformed cells.

The invention relates to application of Col05, Col06 or Col29 protein, the Col05, Col06 or Col29 protein is used for inhibiting or killing insects, the Col05, Col06 or Col29 protein respectively has an amino acid sequence as shown in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5, dicotyledonous plant tobacco is selected as a first host, monocotyledonous plant corn is selected as a second host, the Col05, Col06 or Col29 protein is subjected to plant codon optimization, and the Col05, Col06 or Col29 protein is obtained. And respectively obtaining nucleotide sequences as shown in SEQ ID NO: 2, SEQ ID NO: 4 or SEQ ID NO: 6. Compared with the prior art, the insect inhibition protein Col05, Col06 or Col29 and the application of the coding gene have the advantages that a more effective insect inhibition method, particularly a prevention and control method for diplodia juncoides, is provided, the use of insecticides can be effectively reduced, and the ecological risk can be remarkably reduced. The invention provides a brand-new inhibition method for the diplodia juncosa, the existing genes for resisting the diplodia juncosa are mainly RNAi, and the method provided by the invention is completely different from a method for resisting insects by using proteins in the aspect of insect resistance mechanism.

The invention discloses a dicotyledonous parthenogenesis induction gene RWP and application thereof, and particularly relates to application of the dicotyledonous parthenogenesis induction gene RWP in cultivation of dicotyledonous haploid or haploid induction lines or improvement of haploid induction capability. The invention discovers and verifies that the RWP gene family has the parthenogenesis haploid induction function in the dicotyledon for the first time, and fills the blank of the dicotyledon parthenogenesis haploid induction gene. The invention lays an important foundation for widening the application of haploid breeding technology on dicotyledonous plants and revealing the biological mechanism of parthenogenesis haploid production. In view of the universality of haploid breeding technology utilization in the current breeding industry, the invention has wide application space and prospect in the field of biological breeding.

Apparatus(es), system(s), and method(s) provide for automated separation / extraction and collection of embryonic axes from dicot seeds. Such apparatus(es), system(s), and method(s) can include a rotating suspension of a combination of whole dicot seeds and liquid, wherein the whole dicot seeds are comminuted to substantially separate / extract the embryonic axes. The apparatus(es), system(s), and method(s) can further include through-pores wherein the separated / extracted embryonic axes are generally passed through the through-pores for collection. Embryonic axes separated from seeds can be used for genetic research and experimentation including, but not limited to, biolistic transformation(s), biolistic gene delivery, agrobacterial transformation(s), and / or gene editing.