28 results about "Dicotyledon" patented technology

The invention relates to a haploid induction method based on a brassica napus phospholipase gene BnaPLA2-a and application of the haploid induction method, the BnaPLA2-a gene in brassica napus is knocked out through a gene editing technology, a mutant material capable of inducing haploid generation is obtained, and the application blank of a phospholipase pathway in dicotyledon haploid induction is filled. Compared with a traditional microspore culture technology, the invention provides a brand-new haploid induction path which is derived from the rape and is used for the rape. The invention provides a brand new technical tool and germplasm resources for genetic breeding of brassica napus. Meanwhile, the invention discloses a gene, a mutant, a creation method and application in breeding.

Provided are a prime editing system-based method for achieving inversion editing of a large genomic fragment and a composition used. By means of the efficient multi-gene prime editing protein construct ePPEplus in plants, the inversion of large fragments in plants is achieved by means of designing paired pegRNAs (pegL and pegR), and then by means of processes such as reverse transcription, DNA double-stranded annealing, DNA strand synthesis, and repair. The pegL comprises RTT-L, and the pegR comprises RTT-R. The nucleotide sequence of RTT-L is identical to that of strand A at the right terminus of a double-stranded DNA to be inverted. The nucleotide sequence of RTT-R is identical to that of strand B at the left terminus of said DNA. By means of testing in the monocotyledonous plant wheat and dicotyledonous plants Nicotiana benthamiana and tomato, it is indicated that the method is an effective method for mediating precise inversion of large-fragment DNA in plants.

The present application relates to a promoter pAtEF1 alpha for improving gene editing efficiency of dicotyledon plants and application thereof, and belongs to the field of plant genetic engineering.The pAtEF1 alpha promoter can be used for expression of adenine base editing protein, cytosine base editing protein, Cas nuclease and gRNA.The present application further provides an expression cassette group containing the pAtEF1 alpha promoter and a recombinant vector containing the expression cassette group.The pAtEF1 alpha promoter can be widely applied to the fields of gene editing and base editing of dicotyledon plants, and has wide application in genetic improvement of dicotyledon crops such as tomato and biosynthesis of high-value metabolites.

The invention belongs to the technical field of plant biology, and particularly relates to a protein coded by a larch LarGRF1 gene and application of the protein to plant growth promotion. The invention aims to provide a new choice for plant genetic breeding by exploring growth regulatory factors (GRFs) of larch. The technical scheme of the invention is that the protein coded by the larch LarGRF1 gene has an amino acid sequence as shown in SEQ ID No. 2. Through overexpression of the MarGRF1 gene in dicotyledonous plants and monocotyledonous plants, it is found that an overexpression vector can promote proliferation of plant cells, especially proliferation of calluses and accelerate regeneration and growth.

The application discloses a pyrazole hexacyclic-5-formamide compound containing an aromatic (ethyl) amide group and a preparation and application thereof. A structural general formula of the compound is shown as formula I. The compound is prepared through an amide condensation reaction, and the preparation method is simple. The pyrazole hexacyclic-5-formamide compound containing the aromatic (ethyl) amide group has an obvious inhibiting effect on growth and development of agricultural pests and growth and development of monocotyledonous or dicotyledonous plants. After application, the pests show symptoms of individual atrophy, skin wrinkling, abnormal molting of insect body deformity and even death, and the plants show that the growth of roots and stems is inhibited. The formula I compound can be applied in agriculture as an insecticide and / or herbicide.

This invention discloses a gene for inducing parthenogenesis in dicotyledonous plants. ZbRWP Its applications, specifically, the parthenogenesis-inducing gene in dicotyledonous plants. ZbRWP This invention is the first to discover and verify its application in cultivating haploid or haploid induction lines of dicotyledonous plants or improving haploid induction ability. RWP This gene family possesses parthenogenetic haploid induction function in dicotyledonous plants, filling a gap in the list of parthenogenetic haploid induction genes in dicotyledonous plants. This invention lays an important foundation for broadening the application of haploid breeding technology in dicotyledonous plants and elucidating the biological mechanism of parthenogenetic haploid formation. Given the widespread use of haploid breeding technology in the current breeding industry, this invention has broad application prospects in the field of bio-breeding.

The application discloses a dicotyledon parthenogenesis induction gene BoRWP and application thereof, in particular a dicotyledon parthenogenesis induction gene BoRWP application in cultivating dicotyledon haploid or haploid induction line or improving haploid induction capacity, the application firstly discovers and verifies that RWP a gene family has parthenogenesis haploid induction function in dicotyledon, and fills the blank of dicotyledon parthenogenesis haploid induction gene. The application lays an important foundation for widening application of haploid breeding technology on dicotyledon and revealing biological mechanism of parthenogenesis haploid generation. In view of the wide use of haploid breeding technology in the current breeding industry, the application has wide application space and prospect in the field of biological breeding.

The present invention relates to a process for extracting xyloglucan from the primary cell walls of plant cells, and more particularly from dicotyledonous plants. The process according to the present invention comprises, in particular, a relatively limited number of steps and uses chemical compounds with low human and environmental toxicity. Figure 1

To provide a biodegradable vegetation sheet that allows for the introduction of dicotyledonous plants and is suitable for the growth of dicotyledonous plants. [Solution] A biodegradable vegetation sheet comprising a biodegradable nonwoven fabric body 11 and a biodegradable reinforcing net 15 attached to the surface of the nonwoven fabric body 11, wherein plant seeds 12 and fertilizer 13 are attached to the back surface of the nonwoven fabric body 11, and the nonwoven fabric body 11, which is formed into a sheet by intertwining fibers, is mainly made of animal hair fibers with keratin as the main component, and the animal hair fibers are intertwined with each other by a needle punching method to form the nonwoven fabric body 11 into a sheet, which has slope erosion prevention effect and fertilizer effect, while enabling the germination of plant seeds.

The application discloses a Gliocladium catenatum PE-1, which is classified as Gliocladium catenatum Clonostachys rosea f. catenulata Clonostachys rosea f , is preserved in the China General Microbiological Culture Collection Center on February 2, 2024, and has a preservation number of CGMCC NO. 41164. The application also discloses a bacterial agent prepared from the Gliocladium catenatum PE-1. The application further discloses applications of the Gliocladium catenatum PE-1 and the bacterial agent in preventing and treating plant diseases. The Gliocladium catenatum PE-1 screened in the application can widely induce the burst of active oxygen of a plant immune marker in monocotyledonous plants and dicotyledonous plants, and can effectively prevent and treat various plant diseases, such as soybean phytophthora root rot, rice rice blast, wheat powdery mildew and wheat scab, and provides a new strain resource and technical support for biological control of plant diseases.

The invention discloses an endogenous promoter AMGT1P11 of mangrove plant avicennia marina and application of the endogenous promoter AMGT1P11, and belongs to the technical field of gene engineering, and the nucleotide sequence of the endogenous promoter AMGT1P11 comprises a sequence as shown in SEQ ID NO: 1, a sequence as shown in SEQ ID NO: 2, a sequence as shown in SEQ ID NO: 3 and a sequence as shown in SEQ ID NO: 4; or a sequence which has at least 70% homology with the sequence as shown in SEQ ID NO: 1 and has the same function as the sequence as shown in SEQ ID NO: 1; or, one or more basic groups are added, substituted, inserted or deleted in the sequence shown in SEQ ID NO: 1, and the sequence has the same function as the sequence shown in SEQ ID NO: 1. The AMGT1P11 contains a reported salt-induced cis-acting element GT1-motif (GGTTAA, a new reference can be provided for research of a mangrove plant salt-tolerant mechanism, meanwhile, the promoter can regulate and control expression of exogenous target genes in dicotyledons and monocotyledons, a brand new tool and selection are provided for plant transgenic engineering, and an important foundation is laid for plant genetic improvement.

The invention provides brassinosterols and application of an inhibitor of the brassinosterols in regulating and controlling the de novo regeneration of dicotyledonous plant root organs, and belongs to the technical field of plant growth regulation and control. According to the invention, brassinosteroid (BR) related mutants are used as materials, and the function of BR in the de novo regeneration process of roots is explored through an in-vitro leaf root regeneration induction method. The result shows that the BR signal can inhibit the root regeneration process, based on this, the invention provides the application of the brassinosteroid synthesis inhibitor in promoting generation of root organs of dicotyledonous plant in-vitro tissue and the application in dicotyledonous plant in-vitro culture, the brassinosteroid synthesis inhibitor is exogenously added, and the yield of the brassinosteroid synthesis inhibitor is improved. The rooting process of the dicotyledonous plant in-vitro leaves can be promoted, the in-vitro culture process is accelerated, and the application value is high.

The application discloses a gene CsPOD7 The application belongs to the technical field of molecular breeding and is applied to haploid breeding of cucumber. CsPOD7 The application clones a parthenogenic haploid induction gene CsPOD7 from cucumber, knocks out the gene cspod7 by CRISPR-Cas9 gene editing technology, and crosses the mutant as a male parent with other cucumber materials as female parents to generate parthenogenic haploids. CsPOD7 The application is the first application of developing a parthenogenic haploid induction line in dicotyledonous plants, realizes the application of the haploid induction line based on the gene CsPOD7 CsPOD7 CsPOD7 CsPOD7 CsPOD7 CsPOD7 CsPOD7 CsPO in cucumber genetic breeding, accelerates the breeding process of cucumber, and has great potential application value and market prospect.

The application provides a Lemna australis endogenous strong promoter, a recombinant vector containing the Lemna australis endogenous strong promoter, and application of the Lemna australis endogenous strong promoter and the recombinant vector in starting expression of a target gene in monocotyledonous plants and dicotyledonous plants. The Lemna australis endogenous strong promoter provided by the application has high activity in monocotyledonous plants and dicotyledonous plants, can maintain high activity under conditions of different concentrations of screening agents, and basically does not affect expression of adjacent genes under conditions of different concentrations of screening agents. The application can solve problems of the 35S promoter widely used in the prior art, such as difficulty in further improving activity, low activity in monocotyledonous plants, and influence on expression of adjacent genes.

The invention relates to application of Col05, Col06 or Col29 protein, the Col05, Col06 or Col29 protein is used for inhibiting or killing insects, the Col05, Col06 or Col29 protein respectively has an amino acid sequence as shown in SEQ ID NO: 1, SEQ ID NO: 3 or SEQ ID NO: 5, dicotyledonous plant tobacco is selected as a first host, monocotyledonous plant corn is selected as a second host, the Col05, Col06 or Col29 protein is subjected to plant codon optimization, and the Col05, Col06 or Col29 protein is obtained. And respectively obtaining nucleotide sequences as shown in SEQ ID NO: 2, SEQ ID NO: 4 or SEQ ID NO: 6. Compared with the prior art, the insect inhibition protein Col05, Col06 or Col29 and the application of the coding gene have the advantages that a more effective insect inhibition method, particularly a prevention and control method for diplodia juncoides, is provided, the use of insecticides can be effectively reduced, and the ecological risk can be remarkably reduced. The invention provides a brand-new inhibition method for the diplodia juncosa, the existing genes for resisting the diplodia juncosa are mainly RNAi, and the method provided by the invention is completely different from a method for resisting insects by using proteins in the aspect of insect resistance mechanism.

The invention discloses a dicotyledonous parthenogenesis induction gene RWP and application thereof, and particularly relates to application of the dicotyledonous parthenogenesis induction gene RWP in cultivation of dicotyledonous haploid or haploid induction lines or improvement of haploid induction capability. The invention discovers and verifies that the RWP gene family has the parthenogenesis haploid induction function in the dicotyledon for the first time, and fills the blank of the dicotyledon parthenogenesis haploid induction gene. The invention lays an important foundation for widening the application of haploid breeding technology on dicotyledonous plants and revealing the biological mechanism of parthenogenesis haploid production. In view of the universality of haploid breeding technology utilization in the current breeding industry, the invention has wide application space and prospect in the field of biological breeding.

Apparatus(es), system(s), and method(s) provide for automated separation / extraction and collection of embryonic axes from dicot seeds. Such apparatus(es), system(s), and method(s) can include a rotating suspension of a combination of whole dicot seeds and liquid, wherein the whole dicot seeds are comminuted to substantially separate / extract the embryonic axes. The apparatus(es), system(s), and method(s) can further include through-pores wherein the separated / extracted embryonic axes are generally passed through the through-pores for collection. Embryonic axes separated from seeds can be used for genetic research and experimentation including, but not limited to, biolistic transformation(s), biolistic gene delivery, agrobacterial transformation(s), and / or gene editing.

The invention discloses a method for multi-target editing of Cas12 protein in tobacco and application of the method, and the method comprises the following steps: introducing Cas12i3 mutant protein and a carrier containing a plurality of tRNA-crRNA expression cassettes into tobacco cells together, so that the Cas12i3 mutant protein and crRNA generated by transcription of the tRNA-crRNA expression cassettes have a synergistic effect, and a plurality of targets are efficiently edited in one carrier at the same time. According to the invention, the specific Cas12i3 protein variant is combined with a multi-expression cassette tRNA strategy, so that the problem of low multi-target editing efficiency of the protein in dicotyledonous plants such as tobacco and the like is solved. The invention provides a new method which is simple to construct and efficient to edit, and provides a powerful new tool for genetic improvement of crops.

The utility model discloses a light combined arched farmland flying prevention isolation device, including the isolation cover of coverable setting above the plant, the both ends of isolation cover along width direction are detachably penetrated with support piece, the support piece has the elastic deformation ability, and the support piece after bending deformation can maintain isolation cover is round arch structure setting. The utility model provides technical scheme, sets above the monocotyledon or dicotyledon crop with a detachable isolation device, combines good and fixes above the plant before spraying pesticide or herbicide, can avoid pesticide or herbicide mist drop to scatter to above the plant, can play the protection effect to monocotyledon or dicotyledon crop, and whole can be freely combined according to the size of crop area, and the more wide application range is suitable.

An emulsion composition includes dry powder of dicotyledonous plant seeds in a range between 1 mass % or more and 20 mass % or less, the dry powder having an average particle size in a range between 0.2 μm or larger and 150 μm or smaller, water in a range between 20 mass % or more and 69 mass % or less, and fat and oil in a range between 21 mass % or more and 70 mass % or less.

This invention discloses a gene for inducing parthenogenesis in dicotyledonous plants. RWP Its applications, specifically, the parthenogenesis-inducing gene in dicotyledonous plants. RWP This invention is the first to discover and verify its application in cultivating haploid or haploid induction lines of dicotyledonous plants or improving haploid induction ability. RWP This gene family possesses parthenogenetic haploid induction function in dicotyledonous plants, filling a gap in the list of parthenogenetic haploid induction genes in dicotyledonous plants. This invention lays an important foundation for broadening the application of haploid breeding technology in dicotyledonous plants and elucidating the biological mechanism of parthenogenetic haploid formation. Given the widespread use of haploid breeding technology in the current breeding industry, this invention has broad application prospects in the field of bio-breeding.

The invention belongs to the technical field of crop breeding, and particularly relates to a preparation method and application of a parthenogenesis haploid induction line. According to the parthenogenesis female parent haploid induction gene GEX3 and the application thereof, female parent haploid induction without hybridization is achieved in monocotyledonous and dicotyledonous plants such as arabidopsis thaliana, tomatoes, soybeans and rice by editing a male parent expression gene GEX3, the process is simple, and the induction rate is high.

The application discloses corn ZmDLR9 Gene in regulating plant lateral root development belongs to the technical field of plant genetic engineering and crop genetic improvement. ZmDLR9 The gene can not only play a role in promoting lateral root development in corn, but also can replace the function of a homologous gene in dicotyledonous plants Arabidopsis thaliana. Through a back-up experiment, it is proved that ZmDLR9 The gene can save the lateral root development defect of Arabidopsis thaliana homologous protein APUM24 after loss of function, which indicates that the function of the gene in promoting lateral root development has cross-species conservation, and further proves that ZmDLR9 The gene promotes the development of plant lateral roots, and is not a specific regulation mechanism of corn. Therefore, ZmDLR9 The gene regulates the development of plant lateral roots in monocotyledons and dicotyledons, and has important agricultural production application value for cultivating new varieties with more developed lateral root systems, higher nutrient and water absorption efficiency and stronger stress resistance (such as drought resistance and tolerance to barrenness).

The invention discloses a preparation method of p-hydroxyethyl peptide and application of p-hydroxyethyl peptide in agriculture, and relates to the technical field of agricultural biologication.The p-hydroxyethyl peptide is prepared through an intermolecular dehydration condensation reaction of L-threonine and ethylene glycol under the heating reflux condition; the p-hydroxyethyl peptide can also be prepared by the following steps: carrying out fermentation culture on the Helabeam scrollata, centrifuging, washing, freeze-drying, carrying out methanol ultrasonic extraction to obtain a crude extract, and carrying out macroporous resin and reversed-phase chromatography separation and purification on the crude extract; the preparation process provided by the invention is simple, and the product is easy to purify and has the characteristic of environmental friendliness; the p-hydroxyethyl peptide, the diethyl aminoethyl hexanoate, the brassinolide, the indoleacetic acid and the compound sodium nitrophenolate are compounded for use under a specific concentration, so that the growth and development of crops can be remarkably promoted, and the yield of the crops can be increased; the p-hydroxyethyl peptide-based plant growth regulation composition is widely applicable to crops, is effective to monocotyledonous and dicotyledonous plants, and has broad spectrum.

The application discloses a kind of spirocyclic amino acid skeleton compounds, it is compound shown as formula I with structural general formula, there are 29 kinds of compounds.The application also discloses the preparation method of spirocyclic amino acid skeleton compound.The application also discloses the application of spirocyclic amino acid skeleton compound in as or preparation herbicide, in the application in weed control or preparation in weed control preparation, the control object or weed of the herbicide is selected from the group consisting of barnyard grass, dogtail grass, Chinese alpine paper, crabgrass and other monocotyledonous plants, and purslane, amaranthus retroflexus, goosefoot, solanum nigrum, abutilon theophrasti and other dicotyledonous plants.Spirocyclic amino acid skeleton compound of the application is obtained after molecular modification to microsphaeropsis ochracea ketonic acid, and experimental research shows that the weed control effect is good, especially compound C3, compound C22, compound C23, the growth of crop has no influence basically.Spirocyclic amino acid skeleton compound of the application is suitable for industrial production, and has great application potential.