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Method for improving crop economic characteristic by FCA gene RNA structural zone transgene

An economical trait, transgenic technology, applied in the field of transgenic, can solve problems such as potential that has not been discussed and evaluated

Inactive Publication Date: 2005-05-11
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The use of a structural component of a natural gene to improve the economic traits of crops has not been reported, and its potential has not been discussed or evaluated.

Method used

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  • Method for improving crop economic characteristic by FCA gene RNA structural zone transgene
  • Method for improving crop economic characteristic by FCA gene RNA structural zone transgene
  • Method for improving crop economic characteristic by FCA gene RNA structural zone transgene

Examples

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Embodiment Construction

[0038] Take rice as an example below to further describe the present invention.

[0039] Method and procedure for transgenic rice rFCA gene RRM2 domain:

[0040] 1. Select the segment SEQ.NO3 covering the RRM2 domain in the rFCA full-length cDNA, synthesize primers containing a linker that matches the EcoRI / BamHI multi-cloning site of the expression vector and sequences on both sides of RRM2, and amplify it by PCR. and connected to the expression vector pBI / 520 (the expression vector pBI / 520 is provided by CAMBIA (Center of the Application of Molecular Biology to International Agiculture) in Australia). The rFCA-RRM expression vector pB1 / 520 / rFCA-RRM was obtained. Its physical map see Figure 4 shown. Act1 is the rice actin promoter, which controls the constitutive expression of the gene. Pin2-3 is the gene transcription termination region, including transcription termination signal. 35S is the promoter from cauliflower virus, and Bar is the herbicide resistance gene. No...

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Abstract

The invention was involved in gene engineering field. The method was about using RNA construction region of FCA gene to control blooming to modify crop economic characteristic. Encoded sequences of one or several whole construction region in proteins encoded by FCA whole gene were transferred into target plants. RRM2 construction region covered by rFCA-1 whole gene was amplified by RT-PCR method and was inserted into expression vector pB-1 / 520, then it was transferred into iaponica rice breed medium flower 11.T0 plant with modified gene and T1 (filial generation) showed obvious change on plant shape, leaf shape, size and weight of seeds. The method was applied in economic characteristic of both dicotyledon and monocotyledon.

Description

technical field [0001] The invention belongs to the technical field of transgenics, and in particular relates to a method for improving economic traits of crops by using the RNA structure domain of a flowering control gene (FCA) to transgene. Background technique [0002] At present, the techniques and methods of using transgenes to improve crop economic traits at home and abroad are mainly based on the known relationship between functional genes and phenotypic traits, using constitutive expression vectors or tissue-specific expression vectors to introduce target genes into target plants, in order to obtain Improvement of specific economic traits or stress resistance traits of crops. The genes used in this method are all from natural gene sequence with complete structure or cDNA with complete coding sequence. There is no report on improving the economic traits of crops by using a certain structural component of natural genes, and its potential has not been discussed and eva...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/10C12N15/29C12N15/63C12N15/82
Inventor 杨金水
Owner FUDAN UNIV
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