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Plant stress-resistant associated protein, encoding gene TaERECTA and uses

A technology that encodes genes and proteins, applied in plant genetic improvement, application, plant peptides, etc., can solve problems such as food crops that have not yet been seen, and achieve the effect of improving stress resistance

Inactive Publication Date: 2009-07-29
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, so far, except for AtERECTA, there have been no reports on transpiration efficiency genes in food crops such as wheat and rice

Method used

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  • Plant stress-resistant associated protein, encoding gene TaERECTA and uses
  • Plant stress-resistant associated protein, encoding gene TaERECTA and uses
  • Plant stress-resistant associated protein, encoding gene TaERECTA and uses

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Example 1. Isolation and expression analysis of the gene TaERECTA encoding stress-related proteins in wheat

[0046] 1. Isolation of TaERECTA gene encoding anti-stress related protein

[0047] The wheat used in this example is common wheat (Triticum aestivum L.).

[0048] Construct the full-length cDNA library of wheat, according to the literature (Mao Xinguo et al., 2005, use the improved Cap-trapper method to construct the full-length cDNA library of I. speltii. Acta Genetics, 32 (8): 811-817) The above method is carried out:

[0049] (1) Total RNA extraction and mRNA purification, wheat total RNA was extracted with TRIZOL, and mRNA was separated and purified with oligo(dT) cellulose.

[0050] (2) Synthesis of first-strand cDNA: Mix 10ug mRNA with primer I, add reagents for first-strand cDNA synthesis after denaturation, when the temperature rises to 40°C, add reverse transcriptase, and when the reaction reaches 40 minutes Primer II was added (first strand synthesi...

Embodiment 2

[0091] Embodiment 2, the application of gene TaERECTA in rice

[0092] 1. Construction of transgenic TaERECTA rice

[0093] The construction process of the binary vector pCAMBIA1390-Ubipro: first, the maize genomic DNA was used as a template, and the primer 5'-GCCC was used CTAGGCAGTGCAGCGTGAC-3' (HindIII restriction site) and 5'-GCCAA TTAGTGCAGAAGTAACACCA-3' (PstI restriction site) amplifies the Ubiguntin promoter of maize (with the first intron), then double-digests the target fragment with HindIII and PstI, and connects it to the Ubiguntin promoter cut with HindIII and PstI The pCAMBIA1390-Ubipro vector was obtained from the pCAMBIA1390 vector (Genbank accession number is AF234307).

[0094] Prepare a gene fragment having the sequence shown in sequence 7 in the sequence listing (wherein, the 1st-6th nucleotide at the 5' end of sequence 7 is the recognition site of restriction endonuclease BamH I, the 68th-6th nucleotide at the 5' end The 3001st nucleotide is the coding...

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Abstract

The invention discloses a plant resistance associated protein and encoding gene TaERECTA and application thereof. The protein in the invention is selected from the following (a) or (b): (a) protein consisting of amino acids expressed by sequence 2 in a sequence table; and (b) protein derived from (a) related with plant resistance after substitution and / or deficiency and / or adding of one or several amino acid residues of the amino acid sequences of sequence 2 in the sequence table. Experiments prove that the genes in the invention are introduced in the plant so that the resistance of plants can be improved such as drought resistance and / or salt resistance and / or low temperature resistance. Moreover, the genes in the invention are applied to both monocotyledon and dicotyledon. Therefore, the genes in the invention and the application thereof have important significance for cultivating new types of drought-resistant water-saving, salt-resistant or low temperature-resistant crops, thereby being applied to popularization and application.

Description

technical field [0001] The invention relates to a plant stress resistance-related protein and its coding gene TaERECTA and application. Background technique [0002] Drought is one of the most important limiting factors affecting the world's grain production, and the grain production reduction due to drought accounts for about half of the world's total grain production loss (Gale 2002, Interim Science Council SECRETARIATFOOD AND AGRICULTURE ORGANIZATION OF THE UNITED NATIONS, p1-27). Therefore, the development of water-saving agriculture is a major demand for increasing food production and saving water resources. Among various agricultural water-saving measures, biological water-saving is one of the most economical and effective ways. [0003] Biological water saving refers to improving the water use efficiency (WUE) of plants by exploring and utilizing the characteristics and physiological potential of plants, so as to realize water saving and increase production. WUE is t...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10C12N15/11A01H5/00
Inventor 毛新国景蕊莲昌小平
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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