24 results about "Common wheat" patented technology

The invention discloses application of a wheat disease-resistant factor TatrxH4 in prevention and treatment of gibberellic disease, and belongs to the technical field of genetic engineering, and a coding gene CDS sequence of the wheat disease-resistant factor TatrxH4 is shown as SEQ ID NO: 1; the amino acid sequence of the wheat disease-resistant factor TatrxH4 is as shown in SEQ ID NO: 2; the wheat disease-resistant factor TatrxH4 plays a positive regulation role in immune response of wheat to gibberellic disease, and over-expression of the wheat disease-resistant factor TatrxH4 improves the resistance of wheat to gibberellic disease. According to the invention, an agrobacterium tumefaciens-mediated method and an RNA interference technology are adopted to obtain a wheat strain of an overexpressed disease-resistant factor TatrxH4, and the disease-resistant factor TatrxH4 is identified according to the morbidity condition, disease index and expression condition of disease-resistant related marker genes of an overexpressed plant and common Fielder wheat infected with fusarium graminearum PH-1, so that the disease-resistant factor TatrxH4 is identified. The wheat disease-resistant factor TatrxH4 is known to play a positive regulation role in the immune response of wheat scab resistance, so that the wheat disease-resistant factor TatrxH4 can be used for creating a scab-resistant wheat germplasm material and plays a practical role in prevention and treatment of the wheat scab on the basis of the positive regulation role of the wheat disease-resistant factor TatrxH4.

The invention discloses a roegneria ciliata PmRc1 gene, a protein coded by the roegneria ciliata PmRc1 gene and application of the roegneria ciliata The sequence of the powdery mildew resistance gene PmRc1 is derived from a small fragment translocation line TT-7 created by tetraploid roegneria ciliata and common wheat, the nucleotide sequence of the powdery mildew resistance gene PmRc1 is SEQ ID NO.1, and the amino acid sequence of the powdery mildew resistance gene PmRc1 is SEQ ID NO.2. The roegneria ciliata PmRc1 gene and the recombinant expression vector are applied to cultivation of wheat varieties with powdery mildew resistance. According to the candidate gene of the powdery mildew resistance gene PmRc1 derived from roegneria ciliata, the susceptibility can be enhanced by silencing the gene in a disease-resistant material, and the powdery mildew resistance can be improved by overexpressing the gene. The invention has important significance on wheat powdery mildew resistance breeding.

The invention provides a molecular marker for early detection of tripistil wheat. The molecular marker comprises a nucleotide sequence as shown in SEQ ID NO. 3 and / or SEQ ID NO. 4. The molecular marker provided by the invention can distinguish tripistil wheat from common wheat, has the characteristics of rapidness, high efficiency, accuracy, practicability and the like, and plays an important role in tripistil wheat identification and wheat high-yield molecular breeding.

The invention belongs to the technical field of gene engineering, and discloses a wheat stem rot resistance gene TaRBP3 and application thereof. According to the invention, the wheat stem rot resistance gene TaRBP3 is transferred into common wheat for overexpression by using a transgenic technology for the first time, and the effect of the TaRBP3 gene in wheat stem rot resistance variety breeding is verified, so that the genetic regulation mechanism of wheat stem rot resistance is disclosed; in addition, improvement of wheat varieties by using a genetic engineering technology is promoted, and meanwhile, an important theoretical foundation is laid for breeding of basal stem rot resistant wheat varieties.

The invention belongs to the field of molecular biology, and discloses a molecular marker related to pre-harvest germination resistance QTL Qphs.hbaas-2B. 4 and application of the molecular marker. The applicant performs preharvest germination resistance identification of six environments on a natural population consisting of 235 parts of common wheat by adopting a wheat whole-panicle germination experiment, and performs whole-genome association analysis of preharvest germination resistance on preharvest germination resistance data of the population 1 and 90k chip genotype data of the population 1, so as to obtain a QTL locus Qphs.hbaas-2B. 4 associated with preharvest germination resistance. The site can interpret up to 8.0% of pre-harvest sprouting resistance variation. The KASP detection method constructed by aiming at the molecular marker closely linked with the site is simple and convenient, is low in cost, and can improve the selection efficiency and accuracy of wheat pre-harvest germination resistance screening.

This invention discloses a pre-processing method for high-fiber, high-protein composite whole wheat Chaoshan twisted wheat cakes and the finished product. First, Australian oat flour, common whole wheat flour, black whole wheat flour, and buckwheat whole wheat flour are mixed in a specific ratio and then subjected to low-temperature ultra-fine grinding to prepare a high-fiber, high-protein composite whole wheat flour mixture. Next, this flour mixture is nutritionally formulated with purified water, edible salt, food-grade baking soda, a composite dietary fiber fortifier, and plant protein peptide powder, and then stirred at a controlled temperature to form dough flakes. Following this, the dough flakes undergo a second maturation and rolling process, and then the dough is shaped to resemble hand-twisted dough. Finally, the dough is precisely dried using a three-stage closed-loop hot air drying process, including low-temperature shaping, medium-temperature dehydration, and cooling and moisture retention, to control the final product's moisture content. The finished product is then cooled. By optimizing the raw material ratio, enhancing nutrition, and improving the maturation and drying methods, a healthy Chaoshan twisted wheat cake with high dietary fiber and protein content, stable taste and quality, and a long shelf life is produced.

The invention discloses application of a KASP molecular marker KaspqPDRL-5A in identification of mesosulfuron-methyl resistance of wheat, and belongs to the technical field of molecular marker breeding. The invention aims to accelerate the assisted breeding process of mesosulfuron-methyl-resistant wheat varieties through the KASP molecular marker. The molecular marker is positioned to an SNP (Single Nucleotide Polymorphism) site related to the mesosulfuron-methyl tolerance character of common wheat, the molecular site is located at the 101st basic group from the 5'tail end of a sequence shown in SEQ ID NO.1, and the polymorphism is C / G. A large number of wheat material tests prove that the mesosulfuron-methyl tolerance character of the wheat material with the genotype of CC homozygous at the SNP site is obviously higher than that of the wheat material with the genotype of GG homozygous. The mesosulfuron-methyl resistance of the wheat can be effectively predicted, and the cultivation process of a new herbicide-resistant mesosulfuron-methyl wheat variety is accelerated.

The invention discloses a mesosulfuron-resistant SNP site of a wheat TaASADH-A1 gene, a KASP marker and application, and relates to the technical field of molecular marker-assisted breeding and application of the SNP site, the SNP site takes a common wheat Chinese spring genome Chine Spring RefSeq v1.1 as a reference genome, the SNP is the SNP site of a TraesCS5A02G398300 gene promoter region on a wheat 5A chromosome, and the KASP marker is the KASP marker of the mesosulfuron-resistant SNP site of the wheat TaASADH-A1 gene, the KASP marker is the KASP marker of the mesosulfuron-resistant SNP site of the wheat TaASADH-A1 gene, and the KASP marker is the KASP marker of the wheat TaASADH-A1 gene. Corresponding to the 101st basic group from the 5'tail end of the sequence shown in SEQ ID NO: 1, when the site is GG homozygosis, the corresponding genotype is A; when the site is CC homozygous, the corresponding genotype is B; according to the application, mesosulfuron-resistant wheat is screened or screened in an auxiliary mode, and the mesosulfuron-resistant capacity of wheat is that wheat with the homozygous genotype A is higher than wheat with the homozygous genotype A or wheat with the homozygous genotype B or wheat with the homozygous genotype B in a candidate mode. The developed KASP marker is related to the wheat herbicide-resistant mesosulfuron-methyl, and can be used for screening and identifying the phenotypic character of the wheat herbicide-resistant mesosulfuron-methyl.

This invention belongs to the field of plant genetic engineering and molecular breeding technology, specifically relating to the application of wheat TabHLH93 protein or its encoding gene in regulating plant grain development. This invention constructs a structure capable of ectopic expression... TabHLH93 A binary expression vector for the gene was genetically transformed into common wheat. TabHLH93 Offspring with high gene expression achieved TabHLH93 The gene was overexpressed and verified, demonstrating that TabHLH93 regulates wheat grain size and plays a significant role in regulating agronomic traits such as plant height, tiller number, spike length, and thousand-grain weight. This provides a promising candidate gene for high-yield genetic improvement of wheat, which is of great importance for ensuring food security.

The invention discloses application of a triticale extract in a skin care product in the field of skin care products, and particularly discloses application of the triticale extract in preparation of the skin care product with anti-oxidation, whitening, barrier repairing and anti-inflammatory effects. A preparation method of the triticale extract comprises the steps that triticale is used as a raw material to prepare dough, and the dough is washed into gluten; adding ultrapure water into the gluten to obtain a mixture, performing enzymolysis treatment on the mixture to obtain an enzymolysis product, performing centrifugation and ultrafiltration treatment on the enzymolysis product to obtain filtrate, and performing concentration and spray drying treatment on the filtrate to obtain a dry material. According to the triticale extract and the preparation method thereof, triticale is taken as a raw material, enzymolysis treatment is carried out, the triticale extract with the polypeptide content, the antioxidant effect, the melanin inhibiting effect, the barrier repairing effect and the anti-inflammatory effect superior to those of a common wheat extract is prepared, and a new direction and a theoretical basis are provided for application of triticale in skin care products.

The application discloses a tetraploid long-spike Aegilops tauschii salt-tolerant 3EL chromosome molecular marker and application thereof, and belongs to the technical field of crop genetic breeding. LTTE3EM-2 and LTTE3EZ-1 ) and a specific molecular marker of a long arm of a 6A chromosome of common wheat ( LTTAE6A-2 ) are combined into two groups of molecular markers ( LTTE3EM-2 and LTTAE6A-2, LTTE3EZ-1 and LTTAE6A-2 ), and a primer pair is designed based on the two groups of molecular markers, and it is found through verification that the designed primer pair can be used for identifying the genotype of a salt-tolerant wheat-tetraploid long-spike Aegilops tauschii 3EL translocation line, that is, whether the tetraploid long-spike Aegilops tauschii salt-tolerant 3EL chromosome is homozygous or not can be identified. The application provides an important way for wheat tribe system evolution, germplasm resource identification and molecular marker assisted selection breeding.

The present invention relates to the field of biodegradable, ecologically-friendly consumer goods. In particular, the invention provides a pasta structure comprising at least one enzyme capable of stabilizing the pasta structure, wherein the enzyme is selected from the group comprising a carboxylester hydrolase, a transglutaminase, an oxidase or an oxygenase. The enzyme may reduce or prevent the loss of starch from the pasta when the structure is contacted with water. The pasta structure is suitable for use as a food handling device and may e.g. have the shape of a straw, a bowl, a cup, a plate, a spoon, a fork, a knife, ajar, a funnel, a swizzle stick or a cone. It typically comprises water and wheat selected from the group comprising Triticum aestivum, Triticum durum, Triticum dicoccum, Triticum spelta, Triticum monococcum and a mixture thereof. The invention further provides a method of preparing said pasta structure and a use of at least one enzyme capable of stabilizing a pasta structure, wherein the at least one enzyme is selected from the group comprising a carboxylester hydrolase, a transglutaminase, an oxidase or an oxygenase, for stabilizing the pasta structure. Finally, use of the pasta structure as a food handling device is disclosed, preferably as a drinking straw.

The invention discloses a distant hybridization cultivation method of a hexaploid common wheat disease-resistant and stress-resistant variety. The method comprises the following steps: parent screening and determination, cross pollination and fruiting promotion, hybrid immature embryo rescue and test-tube plantlet cultivation, molecular marker-assisted screening, and homozygous stability and comprehensive character identification. The invention relates to the technical field of agricultural crop breeding. The distant hybridization cultivation method of the hexaploid common wheat disease-resistant and stress-resistant variety reaches the parent screening standard, hybridization and embryo rescue parameters, a molecular marker screening system and the like are clear and controllable, and the distant hybridization cultivation method can be popularized to other wheat distant hybridization breeding scenes; a standardized technical normal form is provided for improving wheat characters by using excellent genes of related species such as rye, and the genetic basis and the resource utilization range of wheat breeding are widened.

The invention discloses a KASP primer group of an aegilops tauschii 4D chromosome, a kit and application, and belongs to the technical field of molecular biology. The kit comprises six KASP primer groups, each primer group comprises two forward primers and one reverse primer, and the specific sequences of the primers are as shown in SEQ ID NO.1-18. After the aegilops tauschii and common wheat are subjected to whole genome comparison, the specific SNP of the aegilops tauschii is obtained through screening, and then the corresponding specific marker is developed, so that the marker developed by the invention can be used for tracking and detecting 4D chromosome introgression fragments or genes which are rich in genetic variation and originated from Straangula type aegilops tauschii AT23; the method has important value for genetic breeding improvement of common wheat.

This invention discloses a common wheat SNP locus marker and its application in detecting lutein content in wheat grains. Specifically, this invention provides the application of substances detecting the Ta-5B-SNP52 locus haplotype in the wheat genome in identifying or assisting in the identification of lutein content in wheat grains. This invention utilizes molecular marker methods to identify a SNP locus, Ta-5B-SNP52, on wheat chromosome 5B. Based on this SNP locus, a molecular marker was developed. Marker / trait association analysis in Chinese wheat microcore germplasm revealed a highly significant correlation between the SNP locus and wheat grain lutein content (P<0.01). Furthermore, this marker exhibits good reproducibility and low cost, facilitating the screening of wheat lines or varieties with higher grain lutein content, which is of great significance for breeding new high-lutein wheat varieties.

The invention belongs to the technical field of wheat breeding, and relates to a molecular marker for detecting haplotype of an aegilops tauschii sourced stripe rust resistant gene Yr28 and application of the molecular marker. According to the invention, a dCAPS marker is designed according to the base difference between the AetAY614Dv1G0004900.1 gene of aegilops tauschii AY61 and the 18th site of a CDS region in the Yr28 allele of aegilops tauschii PI511383, and the marker can effectively identify different haplotypes of the Yr28 gene in the aegilops tauschii germplasm resources. In addition, a KASP marker is developed on the basis of the base difference between the new haplotype of the stripe rust resistance gene Yr28 in the aegilops tauschii AY61 and the TraesAK58CH4D01G004400.1 gene of common wheat AK58, the introgression line genotype created by the common wheat and the aegilops tauschii AY61 can be efficiently identified, and identification and screening of disease-resistant single plants in hybridization or backcross derivative populations are achieved.

The application discloses a heart-nourishing and spirit-soothing medicinal composition and a preparation method and application thereof. The medicinal composition contains 170-190 parts of spina date seed, 30-60 parts of poria cocos, 30-60 parts of longan arillus, 160-190 parts of common wheat, 10-30 parts of fried licorice, 60-130 parts of jujube and 60-130 parts of lily by weight, and the composition is optimized in formula matching to play a synergistic effect, can effectively soothe the nerves and help sleep, can be applied to people of different ages, genders and sleep disorder grades, and the total effective rate is above 80%. The medicinal composition has a long-term curative effect and does not rebound after drug withdrawal. During use, the life habits of patients do not need to be changed, and the compliance of patients is strong.

The present invention discloses the application of a KASP molecular marker Kasp_qPDRL-5A in identifying the trait of wheat resistance to mesosulfuron-methyl, belonging to the technical field of molecular marker breeding. The aim is to accelerate the process of assisted breeding of wheat varieties resistant to mesosulfuron-methyl through this KASP molecular marker. The present invention locates a SNP locus related to the trait of common wheat tolerance to mesosulfuron-methyl, and the molecular locus is the 101st base from the 5' end of the sequence shown in SEQ ID NO.1, with a polymorphism of C / G. Through a large number of wheat material experiments, it is verified that the mesosulfuron-methyl tolerance trait of wheat material with the CC homozygous genotype at this SNP locus is significantly higher than that of wheat material with the GG homozygous genotype. It can effectively predict the resistance of wheat to mesosulfuron-methyl and accelerate the breeding process of new wheat varieties resistant to the herbicide mesosulfuron-methyl.

The invention relates to the technical field of crop molecular biology and molecular breeding, in particular to an HSM1 gene and application thereof in improving wheat growth period related powdery mildew resistance. The HSM1 gene provided by the invention is a powdery mildew-resistant gene explored from wild two-grain wheat, and is transferred into a common wheat variety Yangmai 158 through multiple hybridization and backcross to obtain a BC5F3 disease-resistant family 16-21, and finally the HSM1 gene is obtained. Results of the specific embodiment of the invention show that a strain with increased expression quantity of the HSM1 gene shows growth period dependent powdery mildew resistance, that is, the plant does not resist diseases in a seedling stage, but the resistance is enhanced after an elongation stage, and high resistance even immune resistance is achieved in a heading stage. The resistance mode provided by the HSM1 gene is beneficial for reducing the energy consumption caused by the expression of the anti-disease gene of the plant, and is beneficial for realizing the high-yield target.

The invention discloses a liquid-phase gene chip for identifying genetic infiltration of Teimphy wheat and application of the liquid-phase gene chip, and belongs to the technical field of plant molecular genetic breeding. The chip contains oligonucleotide probes with about 7500 SNP (Single Nucleotide Polymorphism) loci, and covers polymorphic universal SNP markers which are uniformly distributed in the whole genome of wheat, and differential SNP markers which can be used for specifically distinguishing Teimurphy wheat (AtAtGG genome) and common wheat (AABBDD genome). Based on a liquid-phase probe capture technology and in combination with high-throughput sequencing, the genetic infiltration fragment of the Teimurphy wheat in the filial generation can be accurately identified. The chip has the advantages of high specificity, high throughput, high genome coverage, flexibility, economy and the like, can be used for wheat osmotic line identification, excellent gene tracking and the like, and accelerates the wheat breeding process.

The invention relates to the technical field of molecular genetics, in particular to a rye dwarf gene Ddw3 and application thereof. The nucleotide sequence of the Ddw3 gene is as shown in SEQ ID NO. 1. The gene Ddw3 is obtained through separation and screening from dwarf rye germplasm, and the PCR molecular marker for specific amplification is designed for the Ddw3 gene and can be used for rapid identification and tracking of the Ddw3 gene; the Ddw3 gene is introduced into common wheat and rice, so that the plant height can be obviously reduced, and a new way is provided for the creation of dwarf materials and the cultivation of new varieties of crops such as wheat and rice.

Methods and compositions for increasing one or more plant growth characteristics in a plant are disclosed. The compositions comprise one or more microbial isolates that promote plant growth. Methods include providing an effective amount of a composition comprising one or more microbial isolates that promote one or more plant growth characteristics. Aspects of the disclosure are directed to microbial isolates obtained from root nodules of T. aestivum.

The application belongs to the technical field of traditional Chinese medicine component analysis materials, and particularly relates to a construction method of a characteristic spectrum of Yifei Bushen capsules. The traditional Chinese medicine raw material composition of the Yifei Bushen capsules comprises: bran-fried atractylodes, common wheat, poria cocos, ophiopogon, baked licorice, yuzhi, dried tangerine or orange peel, ginger, medlar, cordyceps flower, jujube, hupu lingzhi and ginseng. The characteristic spectrum construction method comprises preparation of a control sample solution and a test sample solution; the control sample solution and the test sample solution are detected by high performance liquid chromatography; the mobile phase of the high performance liquid chromatography is acetonitrile (A) and water (B), and the elution mode is gradient elution; the construction of the characteristic spectrum can be realized under one chromatographic condition, the detection period is greatly shortened, the analysis efficiency is improved, and the method has good specificity, precision, stability, accuracy and the like, can monitor the quality of the finished product, and provides a scientific basis for the standardized research of the Yifei Bushen capsules.

The application belongs to the field of genetic engineering and molecular breeding, and discloses a glycosyl phosphatidylinositol anchor protein gene TaCOBL-A12 in wheat as well as an expression vector and application thereof. The cDNA sequence of the wheat TaCOBL-A12 gene is shown as SEQ ID NO. 2, the coding region DNA sequence is shown as SEQ ID NO. 1, and the amino acid sequence encoded by the gene is shown as SEQ ID NO. 3. The gene is from Triticum asetivum L. Chinese Spring. TaCOBL-A12 is induced to express and enhance in Chinese Spring under high temperature. The gene of the application can significantly improve the heat resistance of Arabidopsis thaliana by genetic transformation. The wheat TaCOBL-A12 of the application can be used in genetic engineering breeding and play an important role in cultivating heat-resistant crop varieties.