33 results about "Common wheat" patented technology

The invention belongs to the technical field of plant genetic engineering molecular breeding, and particularly relates to application of wheat TabHLH93 protein or a coding gene thereof in regulation and control of plant grain development. According to the invention, a binary expression vector capable of realizing ectopic expression of the TabHLH93 gene is constructed and is genetically transformed into common wheat to obtain a progeny with high expression of the TabHLH93 gene, so that overexpression of the TabHLH93 gene is realized, and it is verified that the TabHLH93 has a function of regulating and controlling the size of wheat grains and also has an important regulation and control effect on agronomic characters such as wheat plant height, tiller number, spike length and thousand seed weight. A candidate gene with an important application prospect is provided for wheat high-yield genetic improvement, and the gene has important significance for guaranteeing grain safety.

Disclosed is a primer for amplifying a closely linked molecular marker of a powdery mildew resistance gene PmDR803 of Triticum carthlicum. The molecular marker primer includes a forward primer HENU629-F and a reverse primer HENU629-R, where the nucleotide sequence of the forward primer HENU629-F is shown as SEQ ID NO:1, and the nucleotide sequence of the reverse primer HENU629-R is shown as SEQ ID NO:2. The molecular marker primer provided by the present disclosure can be applied in detection and identification of the gene PmDR803, auxiliary identification of a powdery mildew resistance trait of wheat, and molecular marker-assisted selection breeding.

The invention discloses molecular marker identification and application of dwarf round-grain wheat alleles. The invention belongs to the field of plant breeding, and particularly relates to molecular marker identification and application of dwarf round-grain wheat alleles. The method for identifying or assisting in identifying the wheat dwarf round grains comprises the steps that the genotype of an SNP site in a wheat genome to be detected is detected, the phenotype of the wheat dwarf round grains is identified or assisting in identifying according to the genotype, the SNP site is an SNP site on a wheat 3B chromosome, the nucleotide type of the SNP site is T or C, and the SNP site is the 185th nucleotide of a sequence 1 in a sequence table.

The invention discloses application of a wheat disease-resistant factor TatrxH4 in prevention and treatment of gibberellic disease, and belongs to the technical field of genetic engineering, and a coding gene CDS sequence of the wheat disease-resistant factor TatrxH4 is shown as SEQ ID NO: 1; the amino acid sequence of the wheat disease-resistant factor TatrxH4 is as shown in SEQ ID NO: 2; the wheat disease-resistant factor TatrxH4 plays a positive regulation role in immune response of wheat to gibberellic disease, and over-expression of the wheat disease-resistant factor TatrxH4 improves the resistance of wheat to gibberellic disease. According to the invention, an agrobacterium tumefaciens-mediated method and an RNA interference technology are adopted to obtain a wheat strain of an overexpressed disease-resistant factor TatrxH4, and the disease-resistant factor TatrxH4 is identified according to the morbidity condition, disease index and expression condition of disease-resistant related marker genes of an overexpressed plant and common Fielder wheat infected with fusarium graminearum PH-1, so that the disease-resistant factor TatrxH4 is identified. The wheat disease-resistant factor TatrxH4 is known to play a positive regulation role in the immune response of wheat scab resistance, so that the wheat disease-resistant factor TatrxH4 can be used for creating a scab-resistant wheat germplasm material and plays a practical role in prevention and treatment of the wheat scab on the basis of the positive regulation role of the wheat disease-resistant factor TatrxH4.

The invention discloses a roegneria ciliata PmRc1 gene, a protein coded by the roegneria ciliata PmRc1 gene and application of the roegneria ciliata The sequence of the powdery mildew resistance gene PmRc1 is derived from a small fragment translocation line TT-7 created by tetraploid roegneria ciliata and common wheat, the nucleotide sequence of the powdery mildew resistance gene PmRc1 is SEQ ID NO.1, and the amino acid sequence of the powdery mildew resistance gene PmRc1 is SEQ ID NO.2. The roegneria ciliata PmRc1 gene and the recombinant expression vector are applied to cultivation of wheat varieties with powdery mildew resistance. According to the candidate gene of the powdery mildew resistance gene PmRc1 derived from roegneria ciliata, the susceptibility can be enhanced by silencing the gene in a disease-resistant material, and the powdery mildew resistance can be improved by overexpressing the gene. The invention has important significance on wheat powdery mildew resistance breeding.

The invention provides a molecular marker for early detection of tripistil wheat. The molecular marker comprises a nucleotide sequence as shown in SEQ ID NO. 3 and / or SEQ ID NO. 4. The molecular marker provided by the invention can distinguish tripistil wheat from common wheat, has the characteristics of rapidness, high efficiency, accuracy, practicability and the like, and plays an important role in tripistil wheat identification and wheat high-yield molecular breeding.

The invention discloses application of an elytrigia besa equal-arm chromosome i4JL in maintenance and breeding of a wheat recessive genic male sterile line, and belongs to the technical field of crop genetic breeding and agricultural biology. The invention verifies that the elytrigia besa equal-arm chromosome i4JL has the function of recovering wheat stamen fertility, and creates conditions for developing wheat biological breeding and molecular design breeding by using the elytrigia besa equal-arm chromosome i4JL. The common wheat-elytrigia besa equal-arm chromosome i4JL monomer alien addition line created by the invention is male fertile, selfing and fructification, a wheat cell nucleus male sterile line and a maintainer line can be bred at the same time through selfing, the color difference of formed grains is obvious, optical sorting is easy, and obtaining of a high-purity non-blue-grain male sterile line and a high-purity blue-grain maintainer line is facilitated. The invention provides a novel method for efficiently breeding the high-purity male sterile line and the maintainer line, and a solid foundation is laid for creating a new-generation blue marker type two-line method hybrid wheat production system.

The invention belongs to the technical field of gene engineering, and discloses a wheat stem rot resistance gene TaRBP3 and application thereof. According to the invention, the wheat stem rot resistance gene TaRBP3 is transferred into common wheat for overexpression by using a transgenic technology for the first time, and the effect of the TaRBP3 gene in wheat stem rot resistance variety breeding is verified, so that the genetic regulation mechanism of wheat stem rot resistance is disclosed; in addition, improvement of wheat varieties by using a genetic engineering technology is promoted, and meanwhile, an important theoretical foundation is laid for breeding of basal stem rot resistant wheat varieties.

The invention belongs to the field of molecular biology, and discloses a molecular marker related to pre-harvest germination resistance QTL Qphs.hbaas-2B. 4 and application of the molecular marker. The applicant performs preharvest germination resistance identification of six environments on a natural population consisting of 235 parts of common wheat by adopting a wheat whole-panicle germination experiment, and performs whole-genome association analysis of preharvest germination resistance on preharvest germination resistance data of the population 1 and 90k chip genotype data of the population 1, so as to obtain a QTL locus Qphs.hbaas-2B. 4 associated with preharvest germination resistance. The site can interpret up to 8.0% of pre-harvest sprouting resistance variation. The KASP detection method constructed by aiming at the molecular marker closely linked with the site is simple and convenient, is low in cost, and can improve the selection efficiency and accuracy of wheat pre-harvest germination resistance screening.

This invention discloses a pre-processing method for high-fiber, high-protein composite whole wheat Chaoshan twisted wheat cakes and the finished product. First, Australian oat flour, common whole wheat flour, black whole wheat flour, and buckwheat whole wheat flour are mixed in a specific ratio and then subjected to low-temperature ultra-fine grinding to prepare a high-fiber, high-protein composite whole wheat flour mixture. Next, this flour mixture is nutritionally formulated with purified water, edible salt, food-grade baking soda, a composite dietary fiber fortifier, and plant protein peptide powder, and then stirred at a controlled temperature to form dough flakes. Following this, the dough flakes undergo a second maturation and rolling process, and then the dough is shaped to resemble hand-twisted dough. Finally, the dough is precisely dried using a three-stage closed-loop hot air drying process, including low-temperature shaping, medium-temperature dehydration, and cooling and moisture retention, to control the final product's moisture content. The finished product is then cooled. By optimizing the raw material ratio, enhancing nutrition, and improving the maturation and drying methods, a healthy Chaoshan twisted wheat cake with high dietary fiber and protein content, stable taste and quality, and a long shelf life is produced.

The invention discloses application of a KASP molecular marker KaspqPDRL-5A in identification of mesosulfuron-methyl resistance of wheat, and belongs to the technical field of molecular marker breeding. The invention aims to accelerate the assisted breeding process of mesosulfuron-methyl-resistant wheat varieties through the KASP molecular marker. The molecular marker is positioned to an SNP (Single Nucleotide Polymorphism) site related to the mesosulfuron-methyl tolerance character of common wheat, the molecular site is located at the 101st basic group from the 5'tail end of a sequence shown in SEQ ID NO.1, and the polymorphism is C / G. A large number of wheat material tests prove that the mesosulfuron-methyl tolerance character of the wheat material with the genotype of CC homozygous at the SNP site is obviously higher than that of the wheat material with the genotype of GG homozygous. The mesosulfuron-methyl resistance of the wheat can be effectively predicted, and the cultivation process of a new herbicide-resistant mesosulfuron-methyl wheat variety is accelerated.

The invention belongs to the technical field of traditional Chinese medicine compound preparations, and particularly relates to a qi-tonifying, restlessness-relieving and nerve-calming paste, a preparation method thereof and application of the paste in preparation of a medicine for treating depressive disorder related insomnia. The paste is prepared from the following raw materials in parts by weight: 9-30 parts of spina date seeds, 9-15 parts of rhizoma anemarrhenae, 15-30 parts of Huai wheat, 9-15 parts of honey-fried licorice roots, 12-30 parts of lily bulbs, 15-30 parts of lotus seeds, 10-20 parts of sesame seeds, 10-20 parts of crystal sugar and 3-5 parts of maltose. By reinforcing qi and nourishing yin to consolidate the root, clearing heat and relieving restlessness to calm the symptom and calming the heart and tranquilizing the mind, the three methods are combined to achieve the purposes of tonifying without dryness, clearing without injury, recovering qi and yin, relieving deficiency-heat and calming the mind.

The present application relates to the field of biomolecule detection, in particular to a KASP molecular marker related to the resistance trait of wheat stripe rust, a primer pair and application thereof. The present application carries out whole genome association analysis on 559 common wheat germplasm resources, identifies a stable stripe rust resistance locus on the long arm of wheat 3B chromosome, namely QYr.hzau-3B.1, and develops a KASP molecular marker (KASP molecular marker KASP3B2800) for the locus for screening large-scale materials. The KASP molecular marker provided by the present application provides a new locus and new marker for wheat stripe rust resistance breeding; at the same time, the KASP molecular marker can quickly screen wheat germplasm containing the locus, effectively save time cost, and accelerate the process of wheat resistance germplasm genetic background detection and wheat disease resistance gene assisted selection breeding.

The invention discloses a mesosulfuron-resistant SNP site of a wheat TaASADH-A1 gene, a KASP marker and application, and relates to the technical field of molecular marker-assisted breeding and application of the SNP site, the SNP site takes a common wheat Chinese spring genome Chine Spring RefSeq v1.1 as a reference genome, the SNP is the SNP site of a TraesCS5A02G398300 gene promoter region on a wheat 5A chromosome, and the KASP marker is the KASP marker of the mesosulfuron-resistant SNP site of the wheat TaASADH-A1 gene, the KASP marker is the KASP marker of the mesosulfuron-resistant SNP site of the wheat TaASADH-A1 gene, and the KASP marker is the KASP marker of the wheat TaASADH-A1 gene. Corresponding to the 101st basic group from the 5'tail end of the sequence shown in SEQ ID NO: 1, when the site is GG homozygosis, the corresponding genotype is A; when the site is CC homozygous, the corresponding genotype is B; according to the application, mesosulfuron-resistant wheat is screened or screened in an auxiliary mode, and the mesosulfuron-resistant capacity of wheat is that wheat with the homozygous genotype A is higher than wheat with the homozygous genotype A or wheat with the homozygous genotype B or wheat with the homozygous genotype B in a candidate mode. The developed KASP marker is related to the wheat herbicide-resistant mesosulfuron-methyl, and can be used for screening and identifying the phenotypic character of the wheat herbicide-resistant mesosulfuron-methyl.

This invention belongs to the field of plant genetic engineering and molecular breeding technology, specifically relating to the application of wheat TabHLH93 protein or its encoding gene in regulating plant grain development. This invention constructs a structure capable of ectopic expression... TabHLH93 A binary expression vector for the gene was genetically transformed into common wheat. TabHLH93 Offspring with high gene expression achieved TabHLH93 The gene was overexpressed and verified, demonstrating that TabHLH93 regulates wheat grain size and plays a significant role in regulating agronomic traits such as plant height, tiller number, spike length, and thousand-grain weight. This provides a promising candidate gene for high-yield genetic improvement of wheat, which is of great importance for ensuring food security.

The present invention discloses a black wheat noodle with high nutritional value and a production method, which belongs to the field of food processing. With whole grain black wheat as the core raw material, the shortcomings of existing technologies and products are overcome, the formula of the product is optimized, and the amount of whole grain used is greatly increased, so that the proportion of whole grains exceeds 50%. At the same time, the quality of the product is improved, with good texture properties and flavor characteristics, and a large amount of anthocyanins and antioxidant capacity are maintained. The product of the present invention is rich in various natural products, and its anthocyanin content and antioxidant capacity are successfully retained through high-temperature steam treatment combined with ultrasonic treatment technology. At the same time, its edible quality is not significantly different from that of noodles prepared from ordinary wheat flour.

The invention discloses application of a triticale extract in a skin care product in the field of skin care products, and particularly discloses application of the triticale extract in preparation of the skin care product with anti-oxidation, whitening, barrier repairing and anti-inflammatory effects. A preparation method of the triticale extract comprises the steps that triticale is used as a raw material to prepare dough, and the dough is washed into gluten; adding ultrapure water into the gluten to obtain a mixture, performing enzymolysis treatment on the mixture to obtain an enzymolysis product, performing centrifugation and ultrafiltration treatment on the enzymolysis product to obtain filtrate, and performing concentration and spray drying treatment on the filtrate to obtain a dry material. According to the triticale extract and the preparation method thereof, triticale is taken as a raw material, enzymolysis treatment is carried out, the triticale extract with the polypeptide content, the antioxidant effect, the melanin inhibiting effect, the barrier repairing effect and the anti-inflammatory effect superior to those of a common wheat extract is prepared, and a new direction and a theoretical basis are provided for application of triticale in skin care products.

The application discloses a tetraploid long-spike Aegilops tauschii salt-tolerant 3EL chromosome molecular marker and application thereof, and belongs to the technical field of crop genetic breeding. LTTE3EM-2 and LTTE3EZ-1 ) and a specific molecular marker of a long arm of a 6A chromosome of common wheat ( LTTAE6A-2 ) are combined into two groups of molecular markers ( LTTE3EM-2 and LTTAE6A-2, LTTE3EZ-1 and LTTAE6A-2 ), and a primer pair is designed based on the two groups of molecular markers, and it is found through verification that the designed primer pair can be used for identifying the genotype of a salt-tolerant wheat-tetraploid long-spike Aegilops tauschii 3EL translocation line, that is, whether the tetraploid long-spike Aegilops tauschii salt-tolerant 3EL chromosome is homozygous or not can be identified. The application provides an important way for wheat tribe system evolution, germplasm resource identification and molecular marker assisted selection breeding.

The present invention relates to the field of biodegradable, ecologically-friendly consumer goods. In particular, the invention provides a pasta structure comprising at least one enzyme capable of stabilizing the pasta structure, wherein the enzyme is selected from the group comprising a carboxylester hydrolase, a transglutaminase, an oxidase or an oxygenase. The enzyme may reduce or prevent the loss of starch from the pasta when the structure is contacted with water. The pasta structure is suitable for use as a food handling device and may e.g. have the shape of a straw, a bowl, a cup, a plate, a spoon, a fork, a knife, ajar, a funnel, a swizzle stick or a cone. It typically comprises water and wheat selected from the group comprising Triticum aestivum, Triticum durum, Triticum dicoccum, Triticum spelta, Triticum monococcum and a mixture thereof. The invention further provides a method of preparing said pasta structure and a use of at least one enzyme capable of stabilizing a pasta structure, wherein the at least one enzyme is selected from the group comprising a carboxylester hydrolase, a transglutaminase, an oxidase or an oxygenase, for stabilizing the pasta structure. Finally, use of the pasta structure as a food handling device is disclosed, preferably as a drinking straw.

The invention discloses a distant hybridization cultivation method of a hexaploid common wheat disease-resistant and stress-resistant variety. The method comprises the following steps: parent screening and determination, cross pollination and fruiting promotion, hybrid immature embryo rescue and test-tube plantlet cultivation, molecular marker-assisted screening, and homozygous stability and comprehensive character identification. The invention relates to the technical field of agricultural crop breeding. The distant hybridization cultivation method of the hexaploid common wheat disease-resistant and stress-resistant variety reaches the parent screening standard, hybridization and embryo rescue parameters, a molecular marker screening system and the like are clear and controllable, and the distant hybridization cultivation method can be popularized to other wheat distant hybridization breeding scenes; a standardized technical normal form is provided for improving wheat characters by using excellent genes of related species such as rye, and the genetic basis and the resource utilization range of wheat breeding are widened.

Provided is a use of Thinopyrum bessarabicum isochromosome i4JL in maintaining and propagating a recessive genic male sterile line in wheat, relating to the technical field of crop genetics and breeding and agricultural biology. It has been verified that Thinopyrum bessarabicum isochromosome i4JL has a function of restoring stamen fertility in wheat, thereby establishing a foundation for the utilization thereof in wheat bio-breeding and molecular design breeding. The created common wheat-Thinopyrum bessarabicum isochromosome i4JL monosomic alien addition line exhibits male fertility and self-fruitfulness. Both a wheat nuclear male sterile line and a maintainer line can be simultaneously propagated by means of self-fertilization. The developed seeds show distinct color differences, enabling easy optical sorting, thereby facilitating obtaining a high-purity non-blue-grained male sterile line and a high-purity blue-grained maintainer line. Provided is a novel method for efficiently propagating a high-purity male sterile line and a maintainer line, laying a solid foundation for creating a new generation of blue-marked two-line hybrid wheat production system.

The invention discloses a KASP primer group of an aegilops tauschii 4D chromosome, a kit and application, and belongs to the technical field of molecular biology. The kit comprises six KASP primer groups, each primer group comprises two forward primers and one reverse primer, and the specific sequences of the primers are as shown in SEQ ID NO.1-18. After the aegilops tauschii and common wheat are subjected to whole genome comparison, the specific SNP of the aegilops tauschii is obtained through screening, and then the corresponding specific marker is developed, so that the marker developed by the invention can be used for tracking and detecting 4D chromosome introgression fragments or genes which are rich in genetic variation and originated from Straangula type aegilops tauschii AT23; the method has important value for genetic breeding improvement of common wheat.

This invention discloses a common wheat SNP locus marker and its application in detecting lutein content in wheat grains. Specifically, this invention provides the application of substances detecting the Ta-5B-SNP52 locus haplotype in the wheat genome in identifying or assisting in the identification of lutein content in wheat grains. This invention utilizes molecular marker methods to identify a SNP locus, Ta-5B-SNP52, on wheat chromosome 5B. Based on this SNP locus, a molecular marker was developed. Marker / trait association analysis in Chinese wheat microcore germplasm revealed a highly significant correlation between the SNP locus and wheat grain lutein content (P<0.01). Furthermore, this marker exhibits good reproducibility and low cost, facilitating the screening of wheat lines or varieties with higher grain lutein content, which is of great significance for breeding new high-lutein wheat varieties.

The invention belongs to the technical field of wheat breeding, and relates to a molecular marker for detecting haplotype of an aegilops tauschii sourced stripe rust resistant gene Yr28 and application of the molecular marker. According to the invention, a dCAPS marker is designed according to the base difference between the AetAY614Dv1G0004900.1 gene of aegilops tauschii AY61 and the 18th site of a CDS region in the Yr28 allele of aegilops tauschii PI511383, and the marker can effectively identify different haplotypes of the Yr28 gene in the aegilops tauschii germplasm resources. In addition, a KASP marker is developed on the basis of the base difference between the new haplotype of the stripe rust resistance gene Yr28 in the aegilops tauschii AY61 and the TraesAK58CH4D01G004400.1 gene of common wheat AK58, the introgression line genotype created by the common wheat and the aegilops tauschii AY61 can be efficiently identified, and identification and screening of disease-resistant single plants in hybridization or backcross derivative populations are achieved.

The application discloses a heart-nourishing and spirit-soothing medicinal composition and a preparation method and application thereof. The medicinal composition contains 170-190 parts of spina date seed, 30-60 parts of poria cocos, 30-60 parts of longan arillus, 160-190 parts of common wheat, 10-30 parts of fried licorice, 60-130 parts of jujube and 60-130 parts of lily by weight, and the composition is optimized in formula matching to play a synergistic effect, can effectively soothe the nerves and help sleep, can be applied to people of different ages, genders and sleep disorder grades, and the total effective rate is above 80%. The medicinal composition has a long-term curative effect and does not rebound after drug withdrawal. During use, the life habits of patients do not need to be changed, and the compliance of patients is strong.

The present invention discloses the application of a KASP molecular marker Kasp_qPDRL-5A in identifying the trait of wheat resistance to mesosulfuron-methyl, belonging to the technical field of molecular marker breeding. The aim is to accelerate the process of assisted breeding of wheat varieties resistant to mesosulfuron-methyl through this KASP molecular marker. The present invention locates a SNP locus related to the trait of common wheat tolerance to mesosulfuron-methyl, and the molecular locus is the 101st base from the 5' end of the sequence shown in SEQ ID NO.1, with a polymorphism of C / G. Through a large number of wheat material experiments, it is verified that the mesosulfuron-methyl tolerance trait of wheat material with the CC homozygous genotype at this SNP locus is significantly higher than that of wheat material with the GG homozygous genotype. It can effectively predict the resistance of wheat to mesosulfuron-methyl and accelerate the breeding process of new wheat varieties resistant to the herbicide mesosulfuron-methyl.

The present application belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for strengthening body resistance and tranquilizing the mind, a preparation method and application thereof. The traditional Chinese medicine composition comprises, in mass parts, the following raw materials: 20-50 parts of common wheat, 10-15 parts of female privet fruit, 10-20 parts of cooked date seed, 10-20 parts of ophiopogon japonicus, 10-20 parts of poria, 10-20 parts of baked licorice, 5-15 parts of dried tangerine or orange peel, 10-20 parts of fried lentil, 10-20 parts of white peony root, and 10-30 parts of Chinese yam. The traditional Chinese medicine composition has the effects of soothing liver and relieving depression, and invigorating spleen and nourishing heart, and can relieve self-hemorrhoids, fatigue, heavy head and body, anxiety, depression, insomnia, and significantly improve happiness, health and social function.

The invention relates to the technical field of crop molecular biology and molecular breeding, in particular to an HSM1 gene and application thereof in improving wheat growth period related powdery mildew resistance. The HSM1 gene provided by the invention is a powdery mildew-resistant gene explored from wild two-grain wheat, and is transferred into a common wheat variety Yangmai 158 through multiple hybridization and backcross to obtain a BC5F3 disease-resistant family 16-21, and finally the HSM1 gene is obtained. Results of the specific embodiment of the invention show that a strain with increased expression quantity of the HSM1 gene shows growth period dependent powdery mildew resistance, that is, the plant does not resist diseases in a seedling stage, but the resistance is enhanced after an elongation stage, and high resistance even immune resistance is achieved in a heading stage. The resistance mode provided by the HSM1 gene is beneficial for reducing the energy consumption caused by the expression of the anti-disease gene of the plant, and is beneficial for realizing the high-yield target.

The invention discloses a liquid-phase gene chip for identifying genetic infiltration of Teimphy wheat and application of the liquid-phase gene chip, and belongs to the technical field of plant molecular genetic breeding. The chip contains oligonucleotide probes with about 7500 SNP (Single Nucleotide Polymorphism) loci, and covers polymorphic universal SNP markers which are uniformly distributed in the whole genome of wheat, and differential SNP markers which can be used for specifically distinguishing Teimurphy wheat (AtAtGG genome) and common wheat (AABBDD genome). Based on a liquid-phase probe capture technology and in combination with high-throughput sequencing, the genetic infiltration fragment of the Teimurphy wheat in the filial generation can be accurately identified. The chip has the advantages of high specificity, high throughput, high genome coverage, flexibility, economy and the like, can be used for wheat osmotic line identification, excellent gene tracking and the like, and accelerates the wheat breeding process.

The invention relates to the technical field of molecular genetics, in particular to a rye dwarf gene Ddw3 and application thereof. The nucleotide sequence of the Ddw3 gene is as shown in SEQ ID NO. 1. The gene Ddw3 is obtained through separation and screening from dwarf rye germplasm, and the PCR molecular marker for specific amplification is designed for the Ddw3 gene and can be used for rapid identification and tracking of the Ddw3 gene; the Ddw3 gene is introduced into common wheat and rice, so that the plant height can be obviously reduced, and a new way is provided for the creation of dwarf materials and the cultivation of new varieties of crops such as wheat and rice.