18 results about "Gene gun" patented technology

The invention relates to the technical field of plant drought tolerance, in particular to a plant drought tolerance related protein FYVE4 and application of a coding gene thereof. By inhibiting expression or protein activity of the FYVE4 gene in the plant, the survival rate of the plant under drought stress is increased; according to the method, in genetic improvement of arabidopsis thaliana, after genetic transformation is conducted through an agrobacterium-mediated method or a gene gun method, the stomata closing response speed and chlorophyll accumulation amount of crops can be effectively increased, and therefore plants with stable genetic drought resistance characters are established; the technical scheme is particularly suitable for constructing a water-saving agricultural system, has very important significance for improving crops and cultivating drought-resistant crops, and is suitable for popularization and application.

This invention discloses the TaJDR1 gene and its application in improving plant resistance to Fusarium head blight and drought. The nucleotide sequence of the TaJDR1 gene is shown in SEQ ID NO.1, and its encoded amino acid sequence is shown in SEQ ID NO.2. This invention is the first to clone the TaJDR1 gene from the local wheat variety Wangshuibai and achieve overexpression of this gene in wheat using gene gun-mediated genetic transformation technology. The results show that the transgenic wheat exhibits significantly improved resistance to Fusarium head blight and drought resistance. Since the TaJDR1 gene originates from wheat itself, overexpression does not affect food safety; therefore, this invention can be widely applied in the field of disease and drought resistance breeding for various plants.

This invention discloses a method and application for improving wheat drought stress resistance based on the transcription factor TaHY5, relating to the fields of plant genetic engineering, wheat genetic breeding, and crop stress resistance improvement. This invention involves constructing a TaHY5 overexpression vector or a CRISPR / Cas9 editing vector, and transforming wheat immature embryos / callus tissue using Agrobacterium-mediated transformation or gene gun methods to obtain transgenic and gene-edited materials. After drought stress treatment, indicators such as biomass, malondialdehyde (MDA) content, and electrolyte permeability are measured to comprehensively evaluate drought resistance. Results show that TaHY5 overexpression significantly increases wheat biomass, APX, CAT and other antioxidant enzyme activities, as well as soluble protein and GSH content under drought stress, while reducing MDA accumulation and electrolyte permeability, and enhancing photosynthetic maintenance, reactive oxygen species scavenging, osmotic regulation, and cell membrane stability. This invention is the first to demonstrate that TaHY5 can synergistically improve wheat drought resistance, providing a simple and comprehensive improvement scheme, and offering new gene resources and practical technical pathways for wheat drought-resistant breeding.

The invention discloses a kelp gametophyte gene editing method and application, and belongs to the technical field of gene editing, the kelp gametophyte gene editing method comprises the following steps: preparing a kelp gametophyte receptor; assembling an RNP complex and preparing a microcarrier; introducing the RNP compound into a kelp gametophyte by using a gene gun; and recovering culture and screening. According to the technical scheme, the dependency of a microinjection technology on precise instruments and professional operation skills is eliminated, efficient gene delivery can be achieved through a standardized gene gun operation process, the technical threshold is greatly reduced, and the kelp gametophyte gene editing method has remarkable technical advantages. According to the method, the high-throughput characteristic of a gene gun technology is fully exerted, multiple mutants can be obtained through one-time bombardment, the experiment efficiency is greatly improved, and powerful support is provided for kelp large-scale gene function research and molecular breeding.

The invention discloses a method for wheat genetic transformation based on Cas9 and Cas12i3 gene editing systems, and belongs to the field of gene editing technology application. The method comprises the following steps: inducing wheat grain immature embryos on an induction culture medium to form calluses, and then transferring the calluses to a hypertonic culture medium for culture; then performing gene gun bombardment, wherein bombardment bullets are gold powder particles wrapping the gene editing delivery carrier; the gene editing delivery carrier is a Cas9 delivery carrier or a Cas12i3 delivery carrier; carrying out resistance screening on the calluses bombarded by the gene gun, transferring the non-browned calluses to a differential medium, and culturing until new leaves are differentiated; and transferring the new leaves to a rooting culture medium, and culturing until roots grow into complete plants. The invention provides a whole set of operation method for completing gene editing in Zheng wheat varieties based on gene gun mediation, and provides technical support for application and popularization of a gene editing technology in wheat main pushing varieties.

The invention discloses a pseudo-sorghum genetic transformation method and application. The pseudo-sorghum genetic transformation method comprises the steps of pseudo-sorghum mature seed explant pretreatment, induction culture, gene gun transformation, screening culture, differentiation culture, budding culture, rooting culture and the like. According to the method disclosed by the invention, the callus is successfully induced from the pseudo-sorghum seed immature embryo, the inductivity is high, a simple and efficient genetic transformation method is established, target editing of a pseudo-sorghum gene editing plant can be realized, and the transformation efficiency and the regeneration capacity are high.

The invention discloses an agrobacterium rhizogenes-mediated rapid and efficient genetic transformation method for cynodon dactylon. The method comprises the following steps: (1) transforming, culturing and treating agrobacterium rhizogenes; (2) disinfecting and treating stolon sections of cynodon dactylon; (3) impregnating the stolons of the cynodon dactylon with agrobacterium rhizogenes, and inducing to form hairy roots; and (4) regenerating the transgenic cynodon dactylon plant. Compared with an existing universal genetic transformation method of the cynodon dactylon mediated by a gene gun and agrobacterium tumefaciens, the method has the advantages that tedious and low-efficiency callus induction and regeneration operation does not need to be carried out, the whole operation process can be completed within two months, and a convenient technical means is provided for genetic breeding and gene function research of the cynodon dactylon.

1. The name of the design product: gene gun beauty instrument (MJF-06). 2. The use of the design product: instrument for human beauty. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The invention discloses a gene gun mediated sugarcane genetic transformation method and a special culture medium thereof, and belongs to the technical field of biology. The invention aims to solve the technical problem of how to carry out sugarcane genetic transformation. The sugarcane genetic transformation method comprises the following steps: inducing sugarcane leaf sheaths or tillering plants on a callus induction culture medium to obtain calluses, and bombarding the calluses together with DNA to be transformed and regeneration factors to realize sugarcane genetic transformation, the callus induction culture medium contains 2 mg / L of 2, 4-D, 0.5 g / L of acid hydrolysis casein, 0.5 g / L of inositol, 1.2 mg / L of copper sulfate pentahydrate, 3.5 g / L of plant gel, 5 [mu] M of silver nitrate, 200 mg / L of ascorbic acid and 2 mg / L of picloram, and the pH value is 5.8; and the regeneration factor is TamGRF4-GIF1 or SomGRF4-GIF1, and the regeneration factor is TamGRF4-GIF1. The sugarcane genetic transformation method and the special culture medium thereof can be used for sugarcane genetic transformation.

The application provides a method for establishing a gene gun-mediated high-efficiency transgenic system of Vaccinium. The method comprises the following steps: taking sterile Vaccinium tender leaves as explants, and optimizing the treatment method of high-osmotic medium on the explants; a plasmid vector used for transfection comprises a first expression vector containing a transposase and a second expression vector used for integrating a target gene; after the target gene is integrated into the second expression plasmid, the first expression plasmid is mixed to prepare DNA-coated microprojectiles; the DNA-coated microprojectiles are bombarded on the explants under sterile conditions; the bombarded explants are placed on a regeneration medium to induce sprouting, and positive transgenic plants are obtained through pressure screening. The method can effectively improve the transgenic efficiency of Vaccinium, is not limited by species, can realize high-efficiency genetic transformation of various wild blueberries, greatly shortens the obtaining period of transgenic plants, and fills the key technical blank in the field.

The application discloses a method for increasing drought adaptability of plant osmotic regulation substance accumulation, and belongs to the field of plant genetic engineering and drought-resistant molecular breeding. First, a plant to be transformed is combined with 13C-glutamic acid and 15N-choline double isotope labels to quantize carbon and nitrogen flow redistribution of proline synthesis pathways and glycine betaine synthesis pathways in leaf cells and vascular bundle companion cells, and to identify rate-limiting enzyme nodes. Second, a multi-cistronic plant expression vector is constructed. A first expression box is driven by a leaf-specific promoter and a drought response cis-element composite promoter to drive P5CS. A second expression box is driven by a companion cell-specific promoter and a drought response cis-element composite promoter to drive BADH. Positive transformants are obtained through Agrobacterium mediation or gene gun transformation and molecular screening. The expression quantity of the positive transformants is increased by 5 to 50 times under stress and falls back to a baseline within 72 hours of rehydration. Root activity is significantly improved, and yield is preserved.

Apparatus(es), system(s), and method(s) provide for automated separation / extraction and collection of embryonic axes from dicot seeds. Such apparatus(es), system(s), and method(s) can include a rotating suspension of a combination of whole dicot seeds and liquid, wherein the whole dicot seeds are comminuted to substantially separate / extract the embryonic axes. The apparatus(es), system(s), and method(s) can further include through-pores wherein the separated / extracted embryonic axes are generally passed through the through-pores for collection. Embryonic axes separated from seeds can be used for genetic research and experimentation including, but not limited to, biolistic transformation(s), biolistic gene delivery, agrobacterial transformation(s), and / or gene editing.

The invention provides a method for establishing a gene gun mediated efficient transgenic system of vaccinium plants. The method comprises the following steps: by taking tender leaves of a sterile vaccinium plant as explants, optimizing a treatment method of a hypertonic culture medium on the explants; the plasmid vector for transgenosis comprises a first expression vector containing transposase and a second expression vector for target gene integration; after integrating the target gene into the second expression plasmid, mixing the target gene with the first expression plasmid to prepare a DNA wrapped micro-elastic body; bombarding the explant by using the DNA coated micro-bombardment under a sterile condition through a gene gun; and putting the bombarded explant on a regeneration culture medium to induce germination, and carrying out pressure screening to obtain a positive transgenic plant. The method can effectively improve the transgenosis efficiency of the vaccinium plant, is not limited by species, can realize efficient genetic transformation of various wild blueberries, greatly shortens the obtaining period of the transgenic plant, and fills the key technical blank in the field.

1. The name of the product of this design: Gene Gun Nebulizer. 2. Application of this design product: for facial skin beauty. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The utility model relates to the technical field of gene gun atomizers, in particular to a gene gun atomizer handle which comprises a first shell, a second shell and a third shell. The infusion tube is installed below the second shell, the second shell is detachably connected with the infusion tube, one end of the infusion tube is matched with the injector, the other end of the infusion tube is connected with the conveying tube, the infusion tube is detachably installed, the conveying tube is connected with the ventilation tube, a leather cup buckle is arranged at the front end of the conveying tube, and ventilation holes are formed in the periphery of the leather cup buckle. On one hand, when the infusion tube is blocked, the infusion tube is more convenient to maintain and replace, and convenience is brought to use; on the other hand, the beautifying liquid can be effectively gathered and prevented from splashing along with airflow, the utilization efficiency of the beautifying liquid is improved, and meanwhile the beautifying liquid is cleaner and tidier.

An accelerator module is connected to an initiator module and generates supersonic waves from subsonic waves generated by the initiator module. The supersonic waves deliver particles to cells in tissues. The accelerator module may include a knocking-detonation transition metal and a detonation material. An example of the knocking-detonation transition metal is copper (I) 5-nitrotetrazolate. Another example of the knocking-detonation transition metal is lead azide. An example of the detonation material is pentaerythritol tetranitrate (PETN).

To provide, in a device for delivering a plurality of doped metal particles to cells, a technique capable of making a speed of the metal particles delivered to the cells higher than that in the prior art.SOLUTION: An accelerator module is connected to an initiator module and generates supersonic waves from subsonic waves generated by the initiator module. The supersonic waves deliver particles to cells in tissues. The accelerator module may include a deflagration-to-detonation transition material and a detonable material. An example of the deflagration-to-detonation transition material is copper (I) 5-nitrotetrazolate. Another example of the deflagration-to-detonation transition material is lead azide. An example of the detonable material is pentaerythritol tetranitrate (PETN).SELECTED DRAWING: Figure 8

The invention provides a method for improving rice yield, saline-alkaline resistance and disease resistance based on a 35S enhancer knock-in technology, and belongs to the technical field of molecular breeding and plant genetic engineering. The invention provides a method for increasing rice OsMYB106 expression through a 35S enhancer knock-in technology so as to improve the rice yield and disease resistance of saline-alkali soil. According to the invention, a DNA fragment containing a 35S enhancer and a plant gene editing vector containing a bZIP structural domain coding gene are used for bombarding rice tissues by using a gene gun, and after the obtained transgenic rice is subjected to salt stress treatment, the stress resistance and yield of the rice under salt and alkali conditions can be greatly improved, the resistance of the rice in rice shutter disease and rice blast infection is improved, and the application prospect is wide. The breeding period of the salt-resistant disease-resistant high-yield rice variety is greatly shortened.