8 results about "Gene gun" patented technology

This invention discloses a method and application for improving wheat drought stress resistance based on the transcription factor TaHY5, relating to the fields of plant genetic engineering, wheat genetic breeding, and crop stress resistance improvement. This invention involves constructing a TaHY5 overexpression vector or a CRISPR / Cas9 editing vector, and transforming wheat immature embryos / callus tissue using Agrobacterium-mediated transformation or gene gun methods to obtain transgenic and gene-edited materials. After drought stress treatment, indicators such as biomass, malondialdehyde (MDA) content, and electrolyte permeability are measured to comprehensively evaluate drought resistance. Results show that TaHY5 overexpression significantly increases wheat biomass, APX, CAT and other antioxidant enzyme activities, as well as soluble protein and GSH content under drought stress, while reducing MDA accumulation and electrolyte permeability, and enhancing photosynthetic maintenance, reactive oxygen species scavenging, osmotic regulation, and cell membrane stability. This invention is the first to demonstrate that TaHY5 can synergistically improve wheat drought resistance, providing a simple and comprehensive improvement scheme, and offering new gene resources and practical technical pathways for wheat drought-resistant breeding.

The invention discloses a method for wheat genetic transformation based on Cas9 and Cas12i3 gene editing systems, and belongs to the field of gene editing technology application. The method comprises the following steps: inducing wheat grain immature embryos on an induction culture medium to form calluses, and then transferring the calluses to a hypertonic culture medium for culture; then performing gene gun bombardment, wherein bombardment bullets are gold powder particles wrapping the gene editing delivery carrier; the gene editing delivery carrier is a Cas9 delivery carrier or a Cas12i3 delivery carrier; carrying out resistance screening on the calluses bombarded by the gene gun, transferring the non-browned calluses to a differential medium, and culturing until new leaves are differentiated; and transferring the new leaves to a rooting culture medium, and culturing until roots grow into complete plants. The invention provides a whole set of operation method for completing gene editing in Zheng wheat varieties based on gene gun mediation, and provides technical support for application and popularization of a gene editing technology in wheat main pushing varieties.

1. The name of the design product: gene gun beauty instrument (MJF-06). 2. The use of the design product: instrument for human beauty. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective drawing.

The application provides a method for establishing a gene gun-mediated high-efficiency transgenic system of Vaccinium. The method comprises the following steps: taking sterile Vaccinium tender leaves as explants, and optimizing the treatment method of high-osmotic medium on the explants; a plasmid vector used for transfection comprises a first expression vector containing a transposase and a second expression vector used for integrating a target gene; after the target gene is integrated into the second expression plasmid, the first expression plasmid is mixed to prepare DNA-coated microprojectiles; the DNA-coated microprojectiles are bombarded on the explants under sterile conditions; the bombarded explants are placed on a regeneration medium to induce sprouting, and positive transgenic plants are obtained through pressure screening. The method can effectively improve the transgenic efficiency of Vaccinium, is not limited by species, can realize high-efficiency genetic transformation of various wild blueberries, greatly shortens the obtaining period of transgenic plants, and fills the key technical blank in the field.

The application discloses a method for increasing drought adaptability of plant osmotic regulation substance accumulation, and belongs to the field of plant genetic engineering and drought-resistant molecular breeding. First, a plant to be transformed is combined with 13C-glutamic acid and 15N-choline double isotope labels to quantize carbon and nitrogen flow redistribution of proline synthesis pathways and glycine betaine synthesis pathways in leaf cells and vascular bundle companion cells, and to identify rate-limiting enzyme nodes. Second, a multi-cistronic plant expression vector is constructed. A first expression box is driven by a leaf-specific promoter and a drought response cis-element composite promoter to drive P5CS. A second expression box is driven by a companion cell-specific promoter and a drought response cis-element composite promoter to drive BADH. Positive transformants are obtained through Agrobacterium mediation or gene gun transformation and molecular screening. The expression quantity of the positive transformants is increased by 5 to 50 times under stress and falls back to a baseline within 72 hours of rehydration. Root activity is significantly improved, and yield is preserved.

Apparatus(es), system(s), and method(s) provide for automated separation / extraction and collection of embryonic axes from dicot seeds. Such apparatus(es), system(s), and method(s) can include a rotating suspension of a combination of whole dicot seeds and liquid, wherein the whole dicot seeds are comminuted to substantially separate / extract the embryonic axes. The apparatus(es), system(s), and method(s) can further include through-pores wherein the separated / extracted embryonic axes are generally passed through the through-pores for collection. Embryonic axes separated from seeds can be used for genetic research and experimentation including, but not limited to, biolistic transformation(s), biolistic gene delivery, agrobacterial transformation(s), and / or gene editing.

The utility model relates to the technical field of gene gun atomizers, in particular to a gene gun atomizer handle which comprises a first shell, a second shell and a third shell. The infusion tube is installed below the second shell, the second shell is detachably connected with the infusion tube, one end of the infusion tube is matched with the injector, the other end of the infusion tube is connected with the conveying tube, the infusion tube is detachably installed, the conveying tube is connected with the ventilation tube, a leather cup buckle is arranged at the front end of the conveying tube, and ventilation holes are formed in the periphery of the leather cup buckle. On one hand, when the infusion tube is blocked, the infusion tube is more convenient to maintain and replace, and convenience is brought to use; on the other hand, the beautifying liquid can be effectively gathered and prevented from splashing along with airflow, the utilization efficiency of the beautifying liquid is improved, and meanwhile the beautifying liquid is cleaner and tidier.

An accelerator module is connected to an initiator module and generates supersonic waves from subsonic waves generated by the initiator module. The supersonic waves deliver particles to cells in tissues. The accelerator module may include a knocking-detonation transition metal and a detonation material. An example of the knocking-detonation transition metal is copper (I) 5-nitrotetrazolate. Another example of the knocking-detonation transition metal is lead azide. An example of the detonation material is pentaerythritol tetranitrate (PETN).