153 results about "Gene gun" patented technology

The invention relates to chitosan (CS) vehicles with chitosan nanoparticles, as well as methods for making such chitosan vehicles and for using them to carry a DNA or proteins by forming CS-DNA or CS-protein complexes. The present invention also relates to CS-DNA or CS-protein complexes being useful for transdermal delivery of DNA or protein with a low-pressure gene gun. In another aspect, the present invention also relates to CS-DNA or CS-protein complexes being useful for transcutaneous delivery of a DNA or protein with a skin patch. Further aspects of the present invention relate to methods for making CS-DNA or CS-protein complexes and for using them for diagnostic, therapeutic and biological industrial applications.

The invention discloses an artificial synthesized Bt insecticidal gene for transgenic anti-insect plants, further, the invention relates to the optimization and transformation systematically to a coding frame and a codon of a CrylAc anti-insect gene, according to an activity analysis to Bt protein in different regions and in accordance with the coding characteristics of the monocotyledons, obtaining anti-insect gene CrylAc-M which can be expressed in a monocotyledons efficiently, with the optimized codon and coding frame. The CrylAc-M gene after being transformed is connected on an expression carrier with CaMV35S-Adhl as promoter, and then the Bt gene CrylAc-Adhl is transferred into a maize inbred line with highly efficient conversion rate by a gene gun co-transformation method. A transgenic progeny is positive through Bt protein testing and a field anti-insect test of the field is tested to be highly resistant.

Disclosed is a transformation method whereby an ability to produce a useful substance of a stramenopile can be improved. The method for transforming a stramenopile comprises transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus Labyrinthula, Altornia, Aplanochytrium, Schizochytrium, Aurantiochytrium, Thraustochytrium, Ulkenia, etc. Said foreign gene, which is a gene relating to tolerance against an antibiotic, a colorimetric protein and / or a fatty acid desaturase (Δ5 desaturase gene, Δ12 desaturase gene and / or ω3 desaturase gene), is transferred by using the electroporation or gene-gun technique.

The invention relates to a tetrahymena cell line containing luciferase gene, a construction method and applications thereof. According to the present invention, a PCR technology is adopted to optimize a N-terminal sequence and a C-terminal sequence of firefly luciferase gene into tetrahymena preference codons, the optimized firefly luciferase gene LUC is linked to a carrier pBX to obtain firefly luciferase gene-containing recombinant plasmid pBX-LUC, a gene gun particle bombardment method is adopted to transform the firefly luciferase gene-containing recombinant plasmid pBX-LUC into tetrahymena thermophila B2086 cells, intracellular homologous recombination and paromomycin resistance screening are performed, and MTT1 gene in the cells are replaced by the LUC gene to obtain the engineered cell line B2086-LUC, wherein the engineered cell line B2086-LUC expresses luciferase under specific heavy metal induction, coloration is performed after a fluorescent substrate D-Luciferin is added, and the enlarged fluorescence intensity is detected on so as to reflect conditions of heavy metal pollutants in a reaction environment through quantitative data, such that the detection method is rapid and sensitive, and easy to operate. The tetrahymena cell line containing luciferase gene has a wide application prospect in biological monitoring of water body environment heavy metal pollution.

The invention belongs to the technical field of plant transgenosis, and particularly relates to a Brassica napus transgenic method. A chloroplast transformation tissue culture system suitable to take the rape cotyledons as an explant is constructed, and a chloroplast transformation transgenic plant is obtained through a gene gun transformation method. The invention also discloses a method for constructing a rape chloroplast specificity expression vector, transforming the rape chloroplast and obtaining the transgenic plant by taking rape chloroplast fragments as homologous recombination fragments. The invention provides a novel pathway for transgenosis of Brassica napus.

The invention provides a method for inducing somatic embryos of corn and belongs to the technical field of biological engineering. The method comprises the following steps of: inducing corn explants in a callus inducing culture medium; transferring the explants undergoing inducing culture to a subculture medium; propagating the somatic embryos in the subculture medium; and putting the propagated somatic embryos into differentiation culture medium for differentiation and seedling. By the method, a plurality of somatic embryos of corn can be induced with high efficiency, and the induced somaticembryos of corn have the characteristics of realizing the differentiation of both poles at the same time by long-time subculture infinite propagation and without the control of exogenous hormones, along with high differentiation rate and seedling rate; and because influence of exogenous hormones is avoided in the differentiation process, the mutation rate of somatic cells is relatively lowered atthe seedling stage of corn. The invention can provide an excellent and practical acceptor system for various trans-genetic methods (such as an agrobacterium-mediated method and a gene gun method).