325 results about "Tetrazole" patented technology

The invention discloses a novel synthetic method for 5-phenyl tetrazole, which includes the following steps: firstly performing acid neutralization to hydrazine hydrate; then adding mixing solution formed by cyanobenzene, water and ethyl alcohol to generate phenyl aminoguanidine salt; adopting sodium nitrite for diazotization to obtain nitrine amidine under the acidic condition; obtaining 5-phenyl tetrazole crude product through closed loop under the alkaline condition; obtaining 5-phenyl tetrazole acceptable product through adopting ethyl alcohol for refining. The method has the advantages of simple technology, simplicity in operation and high product yield.

Disclosed are compounds of formula (I): # imgabs0 # or a salt thereof, wherein: X, Y, Z, R1, R2, R3 and n are as defined herein. Also disclosed are methods of inhibiting the activity of one or both of diacylglycerol kinase alpha (DGK alpha) and diacylglycerol kinase zeta (DGK zeta) using such compounds, as well as pharmaceutical compositions comprising such compounds. These compounds are useful for the treatment of viral infections and proliferative diseases such as cancer.

The invention discloses a chromogenic culture medium for detecting gram-negative bacteria in bovine mastitis and a test plate thereof, the culture medium comprises a nutritional ingredient mixture, a composite inhibitor, a composite chromogenic enzyme substrate and cold water gel, the compound chromogenic enzyme substrate is red tetrazole, 5-bromo-6-chloro-3-indole-beta-D-galactoside, and 5-bromo-4-chloro-3-indole-beta-D-glucopyranoside, and the compound chromogenic enzyme substrate is a compound chromogenic enzyme substrate, and the compound chromogenic enzyme substrate is 5-bromo-6-chloro-3-indole-beta-D-glucopyranoside. The chromogenic culture medium and the test board prepared from the chromogenic culture medium can effectively inhibit the growth of non-gram negative bacteria, have the characteristics of strong specificity, high sensitivity, short detection period, easy observation and low detection cost, and have huge development potential and wide application prospects in microbiological detection.

The present invention provides a tetrazole compound, a pharmaceutical composition thereof and a use thereof. The compound of the present invention has a brand-new structure, a low clearance rate, high plasma exposure, good oral bioavailability, and good pharmacokinetic properties, and is conducive to drug development. The compound of the present invention or a pharmaceutically acceptable salt thereof can be used for preventing and / or treating pain, including neuropathic pain, etc.

The invention discloses an electro-synthesis method of alpha-aminomethyl tetrazole, which comprises the following steps: by taking a platinum sheet as a cathode, a carbon rod as an anode and tetrabutylammonium tetrafluoroborate as an electrolyte under the catalysis of constant current, coupling tertiary amine, isonitrile and azido trimethylsilane by a one-pot method to prepare an alpha-aminomethyl tetrazole derivative, and carrying out post-treatment on the obtained reaction mixed solution, and carrying out column chromatography separation to obtain a pure alpha-aminomethyl tetrazole product. The alpha-aminomethyl tetrazole can be obtained only through one-step reaction, the operation steps are few, a large number of oxidizing agents and additives are not used, the post-treatment process is simple, and the product is easy to separate.

The invention discloses a copper-titanium etching solution and a preparation method thereof, and belongs to the technical field of etching solution preparation. The etching solution is prepared from the following components in percentage by mass: 4.5-7.5% of potassium peroxymonosulfate composite salt, 1.2-2.8% of sodium percarbonate, 1.2-2.8% of benzotriazole-2, 3, 4-triazole-2, 3, 4-triazole-2, 3, 4-triazole-2, 3, 4-triazole-2, 3- The cleaning agent is prepared from the following components in percentage by weight: 1.2%-1.8% of 1, 3-dicarboxylic acid, 0.3%-0.8% of 5-aminotetrazole, 0.15%-0.45% of ethylenediamine tetraacetic acid tetrasodium salt, 0.15%-0.45% of hydroxyethylidene diphosphonic acid, 0.15%-0.45% of cocamidopropyl betaine, 0.3%-0.8% of benzotriazole, 0.15%-0.45% of 2-mercaptobenzothiazole, 0.02%-0.08% of poly-N-isopropylacrylamide, 0.7%-1.8% of potassium perchlorate and the balance of ultrapure water. According to the etching solution, the copper-titanium etching rate can be accurately regulated and controlled, back cutting and titanium residues are effectively inhibited, the stability of the etching solution is improved, and the service life of the etching solution is prolonged.

The invention relates to the technical field of copper foils, in particular to a composite layer carrier copper foil, a preparation method of the composite layer carrier copper foil, a method for easily stripping an ultrathin copper foil with a carrier from a composite layer, the ultrathin copper foil and a preparation method of the ultrathin copper foil. The composite layer comprises a nickel layer and an organic layer of a tetrazole compound from inside to outside, and the tetrazole compound has a structure as shown in the following formula (1): R1 is selected from hydrogen, amino, a tetrazole group or a group containing 1-5 carbon atoms; r1 is selected from hydrogen, sulfydryl, amino or a group containing 1-5 carbon atoms, R2 is selected from hydrogen, sulfydryl, amino or a group containing 1-5 carbon atoms, and the groups containing 1-5 carbon atoms in R1 and R2 are respectively selected from C1-C5 alkyl groups, carboxyl-substituted C1-C5 alkyl groups and sulfenyl-substituted C1-C5 alkyl groups. The composite layer carrier copper foil provided by the invention has the characteristics of easy stripping and stable stripping performance.

The invention relates to the technical field of pesticide residue detection, and discloses a method and a device for detecting the residual quantity of tetratolfenpyrad and metabolite thereof, and the method for detecting the residual quantity of tetratolfenpyrad and metabolite thereof comprises the following steps: step 1, dissolving a sample of tetratolfenpyrad and metabolite thereof in an extraction solvent; then injecting the extracting solution into a solvent bottle on a liquid chromatograph; and secondly, the inserting part is inserted into a bottle head at the top of the solvent bottle, the inserting part is in butt joint with a straight pipe fitting in the solvent bottle, a second magnetic ring on the inserting part is in magnetic connection with a first magnetic ring on the bottle head, then a first liquid conveying pipe on the inserting part is in butt joint with a butt joint part on the hollow block, and assembly of a section of pipeline structure is completed. Through segmented assembly of the liquid conveying pipeline, magnetic ring limiting and branch pipe fitting design, the pipeline connection stability is effectively enhanced, leakage is avoided, it is ensured that a chromatographic analysis experiment is continuously and accurately carried out, the experiment failure risk is reduced, and solvent waste and environmental pollution are reduced.

The invention belongs to the technical field of sensors, and relates to an array sensor based on cerium-based metal organic framework nano-enzyme and detection application of perfluoroalkyl compounds. The array sensor is a Ce-MOF triple enzyme activity array sensor and is provided with a plurality of mutually independent sensing channels; the sensing channel contains a Ce-MOF nano enzyme and a chromogenic substrate; the chromogenic substrates comprise a first chromogenic substrate, a second chromogenic substrate and a third chromogenic substrate, the first chromogenic substrate comprises 3, 3 ', 5, 5'-tetramethyl benzidine, the second chromogenic substrate comprises 2, 4-diaminophenol and 4-aminoantipyrine, and the third chromogenic substrate comprises xanthine, purine oxidase and nitrogen blue tetrazole. The MOF nano-enzyme array sensor constructed by the invention has relatively high sensitivity and selectivity in identification and distinguishing of PFASs compounds, not only can accurately distinguish single PFASs compounds, but also can perform quantitative detection, and has good response to PFASs with different concentrations.

The invention discloses a reagent for detecting the activity of isocitrate dehydrogenase. The reagent consists of a first reagent and a second reagent, the first reagent comprises an electron carrier, isocitric acid or isocitrate, divalent metal ions, a first reagent buffer solution, a first reagent additive, a first reagent preservative and a first reagent surfactant; the divalent metal ions comprise metal ions; the second reagent comprises a dye which comprises tetrazolium salt and has an oxidation state and a reduction state, a cofactor, a second reagent buffer solution, a second reagent additive, a coenzyme stabilizer, a second reagent surfactant and a second reagent preservative; the pH is buffered to 7-9.5 and 5-9 by the first buffer solution and the second buffer solution respectively; the first reagent additive and the second reagent additive comprise divalent metal salt, sugar, sugar alcohol, protein, chelating agent and basic amino acid; the first and second reagent surfactants comprise nonionic and anionic surfactants; the first reagent preservative and the second reagent preservative comprise sodium azide or isothiazolinones. The invention further discloses a corresponding detection method.

The invention provides a preparation method of ultrahigh-purity copper. The preparation method comprises the following steps: providing a first anode, a first cathode and an electrolyte; the electrolyte comprises the following components: a purified copper salt solution, inorganic acid, an oxidizing agent and an additive, the purified copper salt solution comprises purified copper salt and water, the purified copper salt comprises copper sulfate and copper nitrate, and the molar ratio of nitrate to sulfate in the purified copper salt solution is (1.5-9): 1; the additive comprises any one or a combination of at least two of an alcohol compound, polyacrylamide or a tetrazole compound; and the first cathode and the first anode are immersed in the electrolyte for electrochemical deposition, and the ultra-high-purity copper is obtained. The electrolyte composed of the purified copper salt solution, the inorganic acid, the oxidizing agent and the additive is adopted, the proportion of nitrate radicals to sulfate radicals in the purified copper salt solution is controlled, the impurity content of the sulfur element and the silver element in the electrolyte can be controlled, and preparation of the ultra-pure electrolytic copper is achieved.

The present invention provides a novel tetrazole compound, a method for synthesizing the same, and a coupling agent. A tetrazole compound according to the present invention is represented by chemical formula (I). In chemical formula (I), R1 and R2 are the same or different and represent a hydrogen atom or a group represented by -(CH2)m-Si(OR)3−p(OH)p, where R represents a methyl group or an ethyl group, m represents an integer of 1-18, and p represents an integer of 0-3. X represents a phenylene group, -NH-, or -(CH2)n-, and n represents an integer of 0-12. However, R1 and R2 are not simultaneously hydrogen atoms.

The invention discloses a penicillin G acylase mutant with high synthetic activity and low hydrolytic activity and application of the penicillin G acylase mutant, and belongs to the field of bioengineering. The penicillin G acylase gene derived from Escherichia coli is subjected to site-specific mutagenesis, so that the amino acid sequence coded by the penicillin G acylase gene mutates from phenylalanine F to alanine A at the 24th site of an alpha chain, the 146th site of the alpha chain mutates from phenylalanine F to valine V, and the 386th site of a beta chain mutates from serine S to alanine A to obtain the mutant. According to the penicillin G acylase mutant alphaF24A / betaS386A disclosed by the invention, compared with the conversion rate of cefamandole synthesized by catalyzing the reaction of methyl mandelate and 3-(1-methyl-1H-tetrazole-5-yl) thiomethyl-7-aminocephalosporanic acid (7-TMCA) within 4 hours before mutation, the conversion rate of cefamandole synthesized by catalyzing the reaction of methyl mandelate and 3-(1-methyl-1H-tetrazole-5-yl) thiomethyl-7-aminocephalosporanic acid (7-TMCA) is improved by 13.70%, and the yield of a side reaction product mandelic acid is reduced by 82%. The penicillin G acylase mutant disclosed by the invention can be used for catalyzing methyl mandelate and 7-TMCA to synthesize cefamandole while greatly reducing the occurrence of side reactions, and can be applied to preparation of cephalosporin antibiotics.

The invention relates to the technical field of bioengineering, in particular to a penicillin G acylase mutant, polynucleotide, an expression vector and application. The penicillin G acylase mutant is obtained by carrying out site-directed mutagenesis on a wild type penicillin G acylase gene of parent Escherichia coli, and carrying out site-directed mutagenesis on the wild type penicillin G acylase gene to obtain the penicillin G acylase mutant. Phenylalanine (F) at the 24th site of an alpha chain, proline (P) at the 383rd site of a beta chain, threonine (T) at the 384th site of the beta chain, glutamic acid (G) at the 385th site of the beta chain and serine (S) at the 386th site of the beta chain of the penicillin G acylase are introduced and mutated by a whole plasmid PCR (Polymerase Chain Reaction) technology to obtain mutants. When the penicillin G acylase mutant obtained by the invention is used for catalyzing methyl mandelate and 3-(1-methyl-1H-tetrazole-5-yl) thiomethyl-7-aminocephalosporanic acid to synthesize cefamandole, the synthesis activity is improved, and meanwhile, the side reaction rate is greatly reduced.

The invention discloses a surface treatment method of a copper foil, an antioxidant copper foil and a negative electrode of a lithium battery. The anti-oxidation copper foil comprises a copper foil base material and an anti-oxidation layer formed on the copper foil base material. The anti-oxidation layer has a chromium element and is derived from a chromic acid compound, and the anti-oxidation layer has a nitrogen element which is at least partially derived from an aminotetrazole compound and a nitrogen-containing heterocyclic compound. The anti-oxidation copper foil has the following characteristics: (a) the chromium content of the anti-oxidation layer measured by XRF (X-Ray Fluorescence) is 5-35 [mu] g / m < 2 >; (b) the nitrogen content of the anti-oxidation layer measured by XPS is 0.1-10% by mass; (c) analyzing the antioxidant copper foil by headspace-MS (Mass Spectrometry) to determine a C-N signal; and (d) the color difference [Delta] E of the antioxidant copper foil baked at 250 DEG C for 10 minutes is not more than 8. Therefore, the antioxidant copper foil disclosed by the invention has more outstanding heat resistance, so that the antioxidant copper foil is more suitable for being used as a negative electrode material of a lithium battery.

A composition for forming a protective film resistant to a wet etching solution for semiconductors, comprising: component (A): a film-forming component; component (B): at least one of a triazole compound (B-1), a tetrazole compound (B-2), a compound (B-3) having two or more phenolic hydroxyl groups, and a compound (B-4) having two or more non-phenolic hydroxyl groups; and component (C): a solvent.

The invention discloses application of hydrogen-rich water in preparation of a drug for treating Duchenne type muscular dystrophy, and particularly relates to the technical field of medicines.The random control experiment is adopted, homologous c57 mice serve as a normal control group, mdx mice are randomly divided into a model group and a hydrogen-rich water group, and the exercise ability is evaluated through a four-limb holding power test and a rotating bar test; detecting mouse creatine kinase by an enzyme-linked immunosorbent assay; carrying out hematoxylin-eosin, NADH-tetrazole reductase and improved Goori three-color dyeing, and carrying out cytochrome C oxidase, succinate dehydrogenase and SDH-COX combined dyeing to observe the pathological change of the skeletal muscle tissue; the invention discloses that the muscle injury condition of a Duchenne type muscular dystrophy model mouse is improved by the hydrogen-rich water through a mitochondrial protection mechanism, and the hydrogen-rich water has a relatively high application prospect and a relatively high economic value.

The invention relates to an etching liquid composition for a copper-based metal film. The etching liquid composition for the copper-based metal film according to the invention comprises hydrogen peroxide, a hydrogen peroxide stabilizer, tetrazole substituted by amino, tetrazole substituted by (C1-C4) alkyl, an ammonium compound and organic acid containing one carboxyl group.

The invention relates to the technical field of semiconductors, in particular to photoresist stripping liquid and a use method thereof. The cleaning agent comprises the following components in percentage by weight: 70%-90% of an aprotic polar solvent, 10%-30% of organic alkanolamine, 0.01%-1.5% of a metal inhibitor and 0.05%-0.3% of an interfacial agent, the metal inhibitor comprises at least one of a tetrazole derivative and a gallic acid derivative. The metal inhibitor comprises a tetrazole derivative and a gallic acid derivative, so that the stripping capability of the stripping liquid on photoresist can be improved while metal on a wafer is not damaged; the weight ratio of the tetrazole derivative to the gallic acid derivative is (0.5-5): 1, so that the stripping efficiency of the stripping liquid on photoresist can be improved, and the stripping of the photoresist can be completed under the conditions that the temperature is 60-90 DEG C and the time is 10-40 minutes.

The present invention relates to novel heteroaryl-triazole and heteroaryl-tetrazole compounds of the general formula (I), in which the structural elements R1, R2, R3, R4 and R5 have the meaning given in the description, to formulations and compositions comprising such compounds and for their use in the control of animal pests including arthropods and insects in plant protection and to their use for control of ectoparasites on animals.

The invention provides a lead salt modified 5-aminotetrazolyl gas generating agent with low combustion temperature and high combustion rate and a preparation method thereof, the gas generating agent comprises 5-aminotetrazole, strontium nitrate and lead salt, and the mass ratio of the lead salt is 5-10%. According to the invention, the 5-aminotetrazolyl gas generating agent is modified, so that the temperature of the gas generating agent can be reduced, the burning speed can be improved, and the applicability of the gas generating agent as a solid propelling type fire extinguishing device is enhanced.

The invention provides an energetic complex K-BTE and a continuous preparation method and application thereof.The continuous preparation method comprises the steps that 1, 2-bis (tetrazole-5-yl) ethane serves as an initial raw material, a ligand solution and a metal ion salt solution are continuously injected into a continuous flow reaction unit through an injection pump, and energetic complex crystals are obtained through filtering, washing and drying; a novel energetic complex preparation method which is simple and continuous in process and mild in reaction condition is developed, the energetic complex K-BTE which is novel in structure and excellent in comprehensive performance is prepared for the first time, and the energetic complex K-BTE has the characteristic of high thermal stability and also has the advantages of being simple and efficient in process method and safe and controllable in process; the thermal decomposition temperature of K-BTE reaches up to 366.5 DEG C, the actually measured impact sensitivity is greater than 40J, the friction sensitivity is between 240N and 360N, and meanwhile, the thermal decomposition of ammonium perchlorate is catalyzed. Due to the excellent performance of the K-BTE, the K-BTE has huge application potential in the field of catalyzing the combustion efficiency of solid propellants as an energetic complex.

The invention relates to a fused ring high-energy density compound and a preparation method thereof, and belongs to the technical field of energetic materials, a 5-6 fused ring skeleton is adopted, 3-((tetrazole-5-yl) amido)-6-(3, 5-dimethylpyrazole-1-yl)-[1, 2, 4, 5] tetrazine is used as a raw material, and a ring closing reaction, a nitration reaction, an amination reaction and an oxidation reaction are sequentially performed to prepare 3-amino-7-nitro-[1, 2, 4, 5] tetrazine. The invention relates to 1, 2, 4] triazole [1, 5-b] [1, 2, 4, 5] tetrazine-2-oxide. The room temperature single crystal density of the prepared fused ring type high-energy-density compound is 1.973 g / cm < 3 >, the initial thermal decomposition temperature reaches 212 DEG C, the actually measured impact sensitivity is 20 J, the friction sensitivity is 324 N, the calculated detonation velocity is 9584 m / s, and the calculated detonation pressure is 40.0 GPa, which indicates that the fused ring type high-energy-density compound has high theoretical energy level and high safety, and is a high-energy low-sensitivity explosive with great potential.

The invention discloses a supramolecular self-assembled fiber as well as a preparation method and application thereof in cells. The supramolecular self-assembled fiber is obtained by mixing a cis, cis-cyclohexane-1, 3, 5-triformyl hydrazine mother solution, a p-formylphenylboronic acid mother solution and a 5-(2-aminophenyl) tetrazole mother solution in a phosphate buffer solution; the application of the supramolecular self-assembled fiber in cells comprises the following steps: inoculating a pore plate with Haila cells, gently and horizontally shaking, culturing, washing, incubating in a complete DMEM (Dulbecco Modified Eagle Medium) solution of 5-(2-aminophenyl) tetrazole, and washing to obtain pretreated cells; and further incubating and washing the pretreated cells in a complete DMEM (dulbecco's modified eagle medium) solution containing cis, cis-cyclohexane-1, 3, 5-triformyl hydrazine and p-formylphenylboronic acid, so as to obtain the cells enriched with the boron element. Compared with a small molecular boron carrying agent, the supramolecular self-assembled fiber prepared by the invention realizes effective boron element enrichment, and has the advantages of high affinity to tumor cells, good stability and low biotoxicity.

The present application relates to a kind of phenyl-tetrazole oxime derivatives and its preparation method and application, wherein the phenyl-tetrazole oxime derivatives have the structure as shown in formula (I), wherein R 1 , R 2 , T, R a , R b , R c , R d , R e Have the meaning described in the present application;The phenyl-tetrazole oxime derivatives described in the present application have excellent prevention effect on cucumber downy mildew, rice sheath blight, cucumber gray mold, cucumber powdery mildew and the like, and can be further developed as a fungicide for application.

This invention discloses a method for synthesizing cefotiam, using 7-aminocephalosporanic acid (7-ACA) as the parent nucleus and introducing a 4-dimethylaminopyridine-dimethyl carbonate composite catalytic system. The reaction is carried out in a one-pot process involving 3-position nucleophilic substitution, 7-position amidation, and simultaneous deprotection in a green mixed solvent of ionic liquid [BMIM]BF₄-water with 1-(2-dimethylaminoethyl)-1H-5-mercapto-tetrazole and compound I. This method simplifies the process, improves selectivity, produces a high-purity product with stable yield, and is suitable for industrial production.

The present invention relates to a composition ("TSP-DNA Oligo") comprising: a thermostable peptide ("TSP"), wherein the peptide comprises: an amino acid sequence as set forth in SEQ ID NO: 1 or SEQ ID NO: 2 or SEQ ID NO: 3 or SEQ ID NO: 4, or an amino acid sequence with at least about 92% sequence identity to a sequence as set forth in SEQ ID NO; 1, or SEQ ID NO: 2, or SEQ ID NO: 3 or SEQ ID NO: 4; and a "DNA Oligo" linked to the thermostable peptide through a linker, wherein the linker comprises: an azide group, an alkyne group, an isocyanate group, or a tetrazole group; wherein the "DNA oligo" comprises a promoter sequence for a T7, T3, SP6 RNA polymerase, or a known variant of said promoter sequence, or a sequence complementary to said promoter sequence or said variant.

Disclosed herein are compounds of the formula (I) or a pharmaceutically acceptable salt thereof (I) wherein X1 represents a -NH- or -O- group, X3-X2 represents a =CH-CH=C- group, a =CH- N=C- group, or a =N-N- group, Z represents a bridged (C5-C8)cycloalkyl group, R1 represents an oxadiazole group, which may be optionally substituted and partially saturated, a tetrazolyl group, a -COOR7 group, a -OCOR8 group, a hydroxy group or a (C1-C6)alkoxy group, a -NRaRb group, a hetero(C3-C8)cycloalkyl group, a -CONHSO2R9 group, a halogen atom, a -NHCOR8 group, a -CONR8R8' group or a -COR8' group, R2 represents a halogen atom or a hydrogen atom, R4 represents a hydrogen atom, R5 represents various groups or alternatively R4 and R5 form together with the atoms to which they are attached a 5- or 6- membered ring optionally comprising a heteroatom, R represents a halogen atom, and n is 0, 1 or 2. Further disclosed are process for preparing the same, pharmaceutical compositions comprising them as well as said compounds of formula (I) for use in the treatment of cancer and infectious disease caused by viruses or bacteria.

The invention relates to a rubber composition based on at least: an elastomeric matrix, and a zinc oxide-free crosslinking system comprising 1 to 20 parts of a sulfur donor, said sulfur donor being selected from the group consisting of 1-phenyl-5-[(1-phenyl-1H-1,2,3,4-tetrazol-5-yl)disulfanyl]-1H-1,2,3,4-tetrazol of formula 1, 2,2′-dipyridyl disulfide of formula 2, 2,2′-dithienyl disulfide of formula 3, and mixtures of these compounds, Formula 1, Formula 2, Formula 3.

The invention relates to the technical field of energetic material synthesis, and particularly provides 5, 5 '-azotetrazole salt and a preparation method thereof.The preparation method comprises the following steps that 1, pretreated carbon cloth is immersed in a mixed solution formed by dissolving cobalt nitrate hexahydrate, ammonium fluoride and urea in deionized water, after a hydrothermal reaction, the carbon cloth is taken out and calcined in the air atmosphere, and a precursor is obtained; and finally, the cobaltosic oxide electrode material loaded on the carbon cloth is prepared. 2, dissolving 5-aminotetrazole in an alkaline solution to prepare an electrolyte, forming a three-electrode system by taking the electrolyte and cobaltosic oxide loaded on carbon cloth as a working electrode, a platinum sheet as a counter electrode and a mercury / mercuric oxide electrode as a reference electrode, and electrifying to carry out electrochemical reaction; 3, bromine water is dropwise added into the three-electrode system in the electrifying electrolysis process, hydrochloric acid is dropwise added to adjust the pH value after the reaction is finished, extraction and rotary evaporation are conducted, and the final product 5, 5 '-azotetrazole salt is obtained.