73 results about "Transplant tissue" patented technology

A method of determining the voltage and current output required for the application of specific and selective electric and electromagnetic signals to diseased articular cartilage in the treatment of osteoarthritis, cartilage defects due to trauma or sports injury, or used as an adjunct with other therapies (cell transplantation, tissue-engineered scaffolds, growth factors, etc.) for treating cartilage defects in the human knee joint and a device for delivering such signals to a patient's knee. An analytical model of the human knee is developed whereby the total tissue volume in the human knee may be determined for comparison to the total tissue volume of the diseased tissue in the animal model using electric field and current density histograms. The voltage and current output used in the animal model is scaled based on the ratio of the total tissue volume of the diseased tissue of the human to the total tissue volume of the diseased tissue in the animal model and the resulting field is applied to the diseased tissue of the human using at least two electrodes applied to the knee or a coil or solenoid placed around the knee. The voltage of the signal applied to the electrodes, coil or solenoid is varied based on the size of the knee joint; larger knee joints require larger voltages to generate the effective electric field.

The present invention relates to systems and apparatuses for improving quality and viability of biological material, such as harvested adipose cells, stem cells, or other cells or biological components, by treatment of the biological material with membrane-repairing/stabilizing agents or the like and/or mechanical removal of components, such as impurities and/or excess treatment agents. The present invention further relates to systems and apparatuses for transplanting tissue, such as adipose tissue.

The invention discloses a method for the tissue culture of Lycoris haywardii and a rapid propagation technique. The method comprises the following: 1) a step of preparing culture mediums, and components of a basic culture medium and the culture mediums in every stage of tissue culture, as well as the weight of every component contained in each liter are as follows: the basic culture medium comprises MS or 1/2MS, wherein the basic culture medium comprises 20 to 40 g/L of sucrose and 8 g/L of agar and has the pH of 5.8; an induction culture medium comprises MS, 0.2 to 1.0 mg/L of TD and 2 g/L of activated carbon; a proliferation culture medium comprises MS, 3 to 7 mg/L of 6-BA and 0.5 to 2.5 mg/L of NAA; a strong seedling culture medium comprises MS, 0.5 to 2.5 mg/L of 6-BA and 0.5 to 1.5 mg/L of NAA; and a rooting culture medium comprises 1/2MS, 0.5 to 2.5 mg/L of KT, 0.5 to 2.5 mg/L IBA and 2 g/L of activated carbon; 2) a step of selecting and sterilizing explants; 3) a step of carrying out induction culture, proliferation culture, strong seedling culture and rooting culture; and 4) a step of domesticating and transplanting tissue culture seedlings. The method has the advantages that the induction rate of Lycoris haywardii buds reaches over 90 percent; the proliferation rate of each week is over 500 percent; the growth rate of the Lycoris haywardii buds is accelerated; the browning phenomenon of the explants is effectively prevented; and the rooting of the tissue culture seedlings is promoted at the same time when bulblet browning is prevented.

A system and method for determining a disease state and clinical outcome of a sample. A sample is illuminated to produce Raman scattered photons, the Raman scattered photons are assessed to generate a Raman spectroscopic data set representative of the sample, wherein said Raman spectroscopic data set comprises at least one of: a Raman spectra of the sample and a spatially accurate wavelength resolved Raman image of the sample; the Raman spectroscopic data set is evaluated using a chemometric technique to classify the disease state of the sample as: acute, chronic, incipient, or none. In one embodiment, the chemontric technique is principle component analysis. In another embodiment, the sample is obtained prior to transplantation and analysis can determine the likelihood of rejection by a host.

The invention priovidese a new Broussonetia papyrifera mulberry tree hybrid distant hybridization and polyploidization breeding method. The method is characterized in that an inductive hybrid fruit callus doubling distant hybridization polyploid hybrid line is established by an embryo engineering and tissue culture technique of distant hybrids through a reproduction engineering kind improvement technology. The method comprises following steps: 1, obtaining distant hybrid fruits; 2, carrying out callus induction of the hybrid fruits; 3, carrying out colchicine treatment of the callus of the hybrid fruits; 4, carrying out recovery culture of treated calluses; 5, differentiating the calluses into bud seedlings; 6, differentiating the bud seedlings into roots; 7, transplanting tissue culture plants; 8, carrying out comparative observation and selection of survival plants; and 9, carrying out asexual propagation of the selected plants to form a stable kind (line). The method solves the problems comprising slow growth,weak adaptability, unclear trunk and bad material quality of present Broussonetia papyrifera, and allows the new polyploid Broussonetia papyrifera mulberry tree hybrid having the advantages of obvious trunk, strong adaptability and good material quality to be cultivated.

The invention discloses a tissue culture method of strawberries and belongs to the technical field of agricultural biological tissue culture. The tissue culture method comprises the following steps: 1, selecting explants, that is, taking the creeping stem tips of healthy and strong disease-free strawberries, with the sizes of 0.3-0.4 mm as explant stem tips for tissue culture in the afternoon on a sunny day at the fifth to sixth months; 2, preparing culture mediums including (1) a differential medium, (2) a subculture solid medium and (3) a root induction solid medium; 3, sterilizing; 4, inoculating; 5, transplanting tissue culture seedlings. According to the tissue culture method, mercury chloride is selected as a sterilizing agent, so that the sterilizing performance is relatively good and the time is easy to control; large and deep green rooting seedlings each with more than three leaves and a large amount of thick and strong adventitious roots directly growing from stem base are selected, so that the survival rate is relatively high in general; the method is relatively simple in overall performing process and relatively easy to perform, can be used for quickly propagating a large amount of virus-free strawberry seedlings, and is very high in practicality.

The invention relates to a tissue culture method for ornithogalum caudatum. The method comprises the following steps of: preparing culture media, selecting and sterilizing explants, primarily cultivating, inducing adventitious buds, growing of the adventitious buds, performing root induction on the adventitious buds, and domesticating and transplanting tissue culture seedlings. The basic culture medium selects an MS culture medium; the dosage of saccharose is 20-40 g/L; a coagulator is agar powder, and the dosage of the coagulator is 8-9 g/L; the pH of the culture medium is adjusted to 5.7-5.8 before being sub-charged; the initial culture medium comprises 0.1-0.5 mg/L of MS+6-BA and 0.05-0.2 mg/L of NAA; the adventitious bud induction culture medium comprises 2.0-4.0 mg/L of MS+6-BA and 0.05-0.2 mg/L of NAA; and the adventitious bud increment culture medium comprises 0.5-3.0 mg/L of MS+6-BA and 0.05-0.2 mg/L of NAA; and the rooting medium comprises 0-0.5mg/L of MS+IBA, or 0-0.5mg/L of 1/2MS+IBA. The method has the advantages that a built tissue culture and rapid propagation technique system for ornithogalum caudatum can reach a growth coefficient of 3-5, a rooting rate of 100% and a transplanting survival rate of 100%, and the tissue culture seedlings are healthy and even. Therefore, the method is suitable for detoxification and large-scale production of excellent ornithogalum caudatum seedlings.

The invention belongs to the technical field of herbal medicinal plant tissue culture, in particular to a rooting method outside a tissue culture seedling bottle of monochasma sheareri. The method mainly solves the technical problems of long rooting period in the tissue culture seedling bottle, more induced aerial roots, low transplanting survival rate and the like, and includes the following steps: (1) selecting bottle seedlings; (2) strong seedling cultivation; (3) hardening seedlings; (4) transplanting tissue culture seedlings; and (5) post-planting management. The method establishes an effective rooting technology outside the tissue culture seedling bottle of monochasma sheareri and optimizes the tissue culture and rapid propagation system. According to the technology, rooting is not required in a bottle, the seedlings are directly transplanted after hormone treatment, two weeks later, new roots can be seen at the base of the seedlings; after 30 days, the number of roots reaches 5-10, and the root length is 0.5-1.5cm; and in about two months, robust rooted seedlings of monochasma sheareri with a seedling height of about 6-10cm can be obtained. Compared with the traditional rooting in a bottle, the seedling raising period is shortened by one month, and the transplanting survival rate is higher than 85%; and by adopting the technology of the method, the rooting seedling quality is obviously improved, and industrial production can be carried out.

The invention discloses a method for transplanting tissue culture seedlings of dendrobium officinale. The method includes the steps that the tissue culture seedlings of dendrobium officinale are taken out, rinsed, disinfected and then transplanted to a soiless substrate, three to four days after the tissue culture seedlings are field-planted, a vitality element and a nutritional agent are sprayed to leaf surfaces in turn within a month respectively for eight to ten times, the vitality element or the nutritional agent is sprayed every day, and then conventional plantation management is executed. The method for transplanting the tissue culture seedlings of dendrobium officinale has the advantages that the vitality element and the nutritional agent are sprayed to the leaf surfaces of the tissue culture seedlings in turn, vitality of the seedlings is enhanced, the growth vigor of the seedlings is vigorous and robust, leaf color is strong green and glossy, the average height of the seedlings is 15cm after the seedlings are transplanted for half a year, and the average thickness of rootstocks is 0.5cm; disease resistance of the seedlings is improved, and the seedlings are barely diseased; the transplanting survival rate is greatly increased and can reach more than 98%.

The invention relates to a fragrant plantain lily tissue culture seedling vitro-bottle rooting method which comprises the following steps: (1) preparing a rooting agent; (2) preparing a transplant matrix; (3) carrying out bottled seedling selection and seedling hardening; (4) transplanting tissue culture seedlings; (5) managing after transplanting. According to the fragrant plantain lily tissue culture seedling vitro-bottle rooting method, an effective fragrant plantain lily tissue culture seedling vitro-bottle rooting technology is established, and a tissue culture rapid-propagation system is optimized. According to the technology, the tissue culture in-bottle rooting procedure is not needed, rooting and domestication are performed at the same time, the seedling raising period is shortened by more than one month, the rooting rate is greater than 95%, and the transplant survival rate is 100%; the vitro-bottle rooting technology is capable of reducing the production cost by about 40% and saving the tissue culture space by more than 50%, and has great significance in improving the fragrant plantain lily tissue culture industrialization level.

A kit for implantation of material into a body, a kit for extraction of tissue from a body and a tissue transplantation kit. A method for implanting material into a body, a method for extracting tissue from a body and a method for transplanting tissue into a body.

The invention provides a method for obtaining polyploidy paulownia tomentosa by performing tissue culture propagation by taking young flowers as explants. The method comprises the steps of a, selecting the young flowers; b, pretreating and disinfecting the young flowers; c, performing callus induction on the young flowers; d, performing callus differentiation culture; e, differentiating seedlings to obtain roots; f, transplanting tissue culture seedlings; and g, observing and authenticating tissue culture plants. By the adoption of the young flowers serving as the explants, the method has the outstanding advantages of high regeneration capability and polyploidy tissue culture propagation in the period from winter to early spring; the problems that the root propagation rate of the polyploidy paulownia tomentosa is low and the leaf tissue culture propagation seedling differentiation frequency is low are solved; the polyploidy paulownia tomentosa can be propagated in a large scale; the authenticity and the consistency of polyploidy of the tissue culture offspring are determined by a morphology and cytology technology.

The invention discloses a rapid propagation method of a Blumea riparia (Bl.) DC medicinal material. The rapid propagation method comprises the steps: sterilizing an explant of the Blumea riparia (Bl.) DC medicinal material, inducing calluses, differentiating the calluses, proliferating multiple shoots, performing rooting culture on the multiple shoots, and transplanting tissue culture seedlings. The rapid propagation method of the Blumea riparia (Bl.) DC medicinal material disclosed by the invention is suitable for breeding and planting in a factorized scale, and has the advantages of low investment, high seedling culture speed, high survival rate, short period, consistent growth vigor of seedlings and the like, the raw material of the explant is easy to obtain, large in quantity and wide in range, the survival rate of tissue culture reaches 95%, moreover, the hereditary character is stable, the produced herbal material has stable quality, meanwhile, the Blumea riparia (Bl.) DC medicinal material is guaranteed to be stable in source, and the rapid propagation method provides a rapid and effective path to fully exert the ecological benefits and economic benefits of the Blumea riparia (Bl.) DC medicinal material in the southwest region of China.

A device containing transplanted tissue includes a housing, having a chamber configured for insertion into a body of a subject and protecting the transplanted tissue from the subject's immune system. The housing includes an oxygen supply container, a hydrogel layer, a port, and an access port. The oxygen supply container has a chamber defined by top and bottom surfaces and sides, disposed within the chamber of the housing. The top surface and the bottom surface of the oxygen supply container include a gas-permeable membrane. The hydrogel layer has inner and outer surfaces. The inner surface of the hydrogel layer contacts the top surface of the oxygen supply container or the bottom surface of the oxygen supply container. The port is configured to deliver oxygen to the oxygen supply container. The access port is configured to receive an exogenous supply of gas and is fluidly connected to the port.

The invention relates to sinningia speciosa tissue culture propagation and a tuber storage and re-propagation method for sinningia speciosa, wherein the sinningia speciosa tissue culture propagation comprises the steps: culturing a stock plant, selecting and disinfecting an explant, carrying out induced differentiation under the conditions that the culture temperature is 26+/-2 DEG C, the illumination culture time is 11-13 hours per day, and the illumination intensity is 2500-5000lx; carrying out subculture expanding propagation in a 500ml triangular flask; transplanting tissue seedlings, setting the tissue seedlings to obtain sinningia speciosa tissue culture nursery stocks; storing and re-propagating the tissue culture seedlings: namely, culturing propagation-expanded flask seedlings in the 500ml triangular flask under the conditions that the culture temperature is 16-28 DEG C, the illumination intensity is 500-1500lx and the illumination time is 11-12 hours per day, heading in the flask, carrying out tuber subculture storing; and reviving a tuber in the flask and carrying out subculture expanding propagation of the revived tuber in a next period.

The invention discloses a method for mechanically transplanting tissue culture seedlings, and belongs to the technical field of plant tissue cultivation. The method is applied to a strip tissue culture seedling cultured singly in a test tube, and is cultured by a solid-state culture medium in the test tube. The method is used for identifying and locating the node of a tissue culture seedling by utilizing machine vision technology and performing transplanting related operation on the tissue culture seedling by a robot according to the node location, and comprises the steps of seedling selection, cutting and implanting. The method can complete switching from manual transplanting operation to mechanical transplanting operation, so that the manual labor intensity can be reduced, a clean sterile environment without human disturbance is created, the pollution level is reduced, and the seedling survival rate is increased. The method has actual meaning.

The invention provides a method of growing seedling of a kiwi with a red core, belongs to the technical field of planting. The method comprises the following steps: selecting and sterilizing explants, inducing rooting, inducing buds and transplanting tissue culture seedlings. According to the method of growing seedling of the kiwi with the red core, a rooting inducing solution and a bud inducing solution are highly targeted and well applicative. The use of sponges as attachment of the solution enables the explants to have normal aerobic respiration and effectively avoid the explants necrosis caused by the lack of oxygen supply of a traditional culture medium, the rooting inducing culture is designed before the bud inducing culture, the phenomenon of new bus necrosis caused by the lack of nutrition when the traditional bud inducing culture is designed in the first is effectively avoided, and in the buding stage, the unique linear variation design of the light intensity enables the explants to grow buds fast and form the tissue culture seedlings. The tests prove that the rooting rate of the explants of kiwi with the red core is 95% or more and the germination rate is 96% or more, and transplant survival rate is 95% or above by using the method of growing seedling of the kiwi with the red core.

The invention relates to a soilless cultivation method for cultivating miniature seed potatoes through potato virus elimination. The soilless cultivation method includes the step of preparing a seed bed, the step of transplanting tissue culture seedlings, the step of managing rooting of the tissue culture seedlings, the step of preparing nutrient solutions, the step of quantitatively using the different nutrient solutions, the step of controlling the time periods for pouring the nutrient solutions and other steps. The soilless cultivation method is characterized in that the nutrient solutions which are used in soilless cultivation of the seed potatoes are prepared quantitatively, and the times and the time periods of application are set. By means of the soilless cultivation method, the quality, yield and specification of the virus-free miniature seed potatoes are improved, manpower, material resources and financial resources are saved, production cost is reduced, and the ratio of input to output is increased.

The invention discloses a method for planting dendrobium candidum by adopting hydrophilic polyurethane foam. The method comprises the steps: constructing a dendrobium candidum planting greenhouse; preparing a culture medium; transplanting tissue culture seedlings; carrying out water and fertilizer management; harvesting and the like. By adopting hydrophilic polyurethane foam as the culture medium, the problem that the culture medium has poor air permeability and poor water retention at the root position in a fixed planting process of the dendrobium candidum tissue culture seedlings is solved; meanwhile, the dendrobium candidum is cultured by using a selenium-containing nutrient solution, so that the selenium content of the dendrobium candidum is improved and the economical value of the dendrobium candidum is increased.

The invention discloses a method for simply transplanting tissue culture seedlings of eucalyptus urophylla * E. grandis. The method comprises the following steps: (1) transferring the tissue culture seedlings taking root well and a culture bottle from a constant-temperature illumination culture box to the outdoor natural environment so as to acclimatize the seedlings; (2) after the seedlings are acclimatized, using a pair of tweezers to take out the tissue culture seedlings in the culture bottle, and using sterile water to rinse agar on root systems of the tissue culture seedlings; (3) placing the rinsed tissue culture seedlings in potassium permanganate solution, soaking for 30 seconds, and using the sterile water to rinse the tissue the culture seedlings after the tissue culture seedlings are taken out; (4) transplanting the rinsed tissue culture seedlings in a cultivation base material, and using the cultivation base material to cover the roots systems of the tissue culture seedlings during the transplanting process; (5) spraying carbendazim on the tissue culture seedlings for one time after transplantation is finished so as to wet 1/3-1/2 of the upper portion of the cultivation base material; (6) preserving moisture and heat of the tissue culture seedlings until sprouts of the tissue culture seedlings germinate; and (7) transplanting the sprouts of the tissue culture seedlings into the field so as to be cultivated in one week after the sprouts of the tissue culture seedlings germinate. The transplanting survival rate can be over 98%.

The invention discloses a method for panting dendrobium officinale by adopting a tilt-type seedbed, which comprises steps of building a dendrobium officinale planting greenhouse, preparing cultivation substrate, transplanting tissue cultured seedlings, managing water and fertilizer and harvesting dendrobium officinale. According to the invention, hydrophily polyurethane foam is used as the cultivation substrate, so the problems of poor breathability and water-retaining performances during the process of planting and cultivating the polyurethane foam tissue cultured seedlings; at the same time, the tilt-type seedbed are utilized, so the excess nutrient solution can be collected and reused conveniently; the tilt-type seedbed is the first choice; the nutrient solution used in the invention is added with a small amount of selenium, thus facilitating to improving selenium content in dendrobium officinale and increasing economic value of dendrobium officinale.

The invention discloses an improved method for factory transplanting Chinese yam tissue culture seedlings and cultivating seed yams and belongs to the technical field of agricultural production. According to the invention, the tissue culture seedlings are transplanted after seedling hardening; insulation planting boxes which is light transmitting and water conservation and combined with ventilation are adopted during transplanting living plants so that the yam tissue culture seedlings can be adapted to quick external environment change and the transplanting survival rate reaches 71.9%; and then through a series steps of strict seedbed management; finally, the ratio of tissue culture seed yams being 20g and above is 45.3%. According to the invention, by means of the insulation planting boxes for transplanting tissue culture seedlings, the yam tissue culture seedlings transplanting and seed yam reproduction are realized. The method of the invention is simple to operate and the produced seed yams have high quality and the reproduction of seed yam is fast and seed yams have less virus.

The invention belongs to the technical field of forestry vegetative propagation, and particularly relates to a rapid propagation method of Hydrangea macrophylla. The method comprises the following steps: (1) carrying out sterile disinfection treatment by taking a stem section of the Hydrangea macrophylla as an explant; (2) cutting the Hydrangea macrophylla stem section treated in the step (1) intoHydrangea macrophylla stem sections each with at least one axillary bud, inoculating the Hydrangea macrophylla stem sections into a culture medium, and germinating the axillary buds; (3) when the axillary buds in the step (2) extend to a certain length, cutting off the axillary buds, inoculating the axillary buds into a culture medium, and carrying out proliferation on the axillary buds to obtaina large number of axillary buds; (4) inoculating the axillary buds in the step (3) into a culture medium, and carrying out induction culture to form rooting seedlings; and (5) transplanting tissue culture seedlings in the step (4), namely the rooting seedlings, into a matrix, and carrying out culture to obtain Hydrangea macrophylla aseptic seedlings with robust rhizomes. The method has the advantages of being high in propagation speed and large in propagation coefficient, and is not limited by seasons and propagation materials.