13 results about "Transplant tissue" patented technology

This utility model relates to the technical field of planting troughs for transplanting tissue culture seedlings of sugar beets, and more particularly to a planting trough for transplanting tissue culture seedlings of sugar beets. It includes a fixed base plate, with threaded adjusting rods rotatably connected to both sides of the top of the fixed base plate. A fixed top block is fixedly connected to the top of the threaded adjusting rod, and a threaded connecting block is threadedly connected to the outer wall of the threaded adjusting rod. Through the mechanical linkage design of the threaded rotating rod, traction plate, fixed sliding rod, and receiving drip plate, the non-destructive seedling removal function of sugar beet tissue culture seedlings is achieved. The rotating block drives the receiving drip plate to rise smoothly, pushing the seedling and root soil as a whole to the top of the planting cylinder. Operators can directly remove the seedlings without digging, reducing root damage compared to traditional methods. Field trials have verified that the survival rate of sugar beet tissue culture seedlings transplanted using this planting trough is significantly higher than that of traditional planting troughs, and the seedling recovery period is shortened, laying a good foundation for later growth and development, and significantly improving the economic benefits of sugar beet cultivation.

This invention proposes a path planning method, device, and readable storage medium for a robotic arm used for transplanting tissue culture seedlings. This method uses machine vision to identify the position of the tissue culture seedlings and the empty space in the plug tray. It defines a 28-dimensional state space and an 8-dimensional action space encompassing position, posture, joint angles, and gripping force. A multi-sub-item weighted reward function is designed that integrates successful transplanting, obstacle avoidance, posture matching, and time efficiency. A deep reinforcement learning network is constructed using the Soft Actor-Critic (SAC) algorithm. Through virtual simulation training, the robotic arm autonomously learns to generate a collision-free, smooth, and efficient grasping-transferring-transplanting path, adapting to dynamic environments such as the diverse morphology of tissue culture seedlings and varying plug tray sizes. This method eliminates the need for pre-set scenario parameters, significantly improving the system's generalization and automation capabilities, and providing a reliable solution for intelligent production of tissue culture seedling transplanting.

A method for maintaining tissue for transplant comprises: mounting the tissue on a tissue platform and preparing it for connection to a tissue enclosure; coupling the orifices of the tissue with pre-selected locations on the tissue platform through tubing and tissue platform connectors; directing venous output from the tissue into a fluid reservoir within the tissue enclosure; coupling the tissue platform connectors to a perfusion system; placing the tissue platform into the tissue enclosure, leaving an air space between the fluid reservoir and the tissue platform; measuring output from the tissue; recirculating perfusate from the fluid reservoir back into an artery of the tissue; monitoring and regulating the temperature of the perfusate prior to pumping the perfusate into the tissue; pumping a gas mixture into an oxygenation device configured to extract oxygen from the gas mixture and supply the oxygen to the perfusate as dissolved oxygen; and monitoring vitality of the tissue through monitoring a status of tissue resistance pressure and flow changes, oxygen consumption, carbon dioxide, and pH.

The invention relates to a tissue culture and rapid propagation method of scabiosa cochinchinensis, which belongs to the technical field of plant tissue culture and rapid propagation, and is characterized by comprising the following steps: S1, preparing a culture medium; the culture medium comprises an MS culture medium, a callus induction culture medium, a callus differentiation proliferation culture medium and a rooting induction culture medium; s2, performing induced callus culture; inoculating the explant sample into a callus culture medium until a callus tissue is formed; s3, callus differentiation and proliferation; inoculating explants with calluses into the callus differentiation and proliferation culture medium, and culturing until bud clusters are formed; s4, induced rooting culture; the bud clusters are inoculated into induced rooting culture for rooting culture; s5, transplanting tissue seedlings; after the plant height of the bud clusters is larger than 4 cm, the bud clusters are transplanted into a matrix, the environment humidity is guaranteed, and normal cultivation is conducted; the substrate is formed by mixing peat soil, perlite and sand according to the volume ratio of 4: 3: 2.

The present disclosure relates to the engraftment and proliferation of cells in adipose-associated lymphoid cell collections ("FALCs" or "milts"), which can be used to generate functional ectopic tissue. The present disclosure further provides methods and compositions for grafting and expanding cells in FALCs. The present disclosure also provides methods and compositions for establishing ectopic liver tissue in FALCs and using such ectopic liver tissue for therapeutic benefit, and methods and compositions for generating ectopic kidney tissue in FALCs, which can be used in a subject for therapeutic benefit. [Solution] A method for generating ectopic tissue in a subject, the method comprising introducing one or more cells into an adipose-associated lymphocyte accumulation in the subject and providing one or more agents that promote the formation of ectopic tissue.

The utility model discloses a savatier monochasma herb tissue culture seedling transplanting auxiliary device which comprises a handheld part and two working heads installed on the handheld part, the two working heads are symmetrically arranged, one end of each working head is a seedling taking head, the other end of each working head is a planting head, and the lower end of each seedling taking head is provided with a steel wire strip used for taking out tissue seedlings in a culture bottle. The planting head comprises a conical shell used for opening matrix soil and planting tissue seedlings. According to the auxiliary device for transplanting the savatier monochasma herb tissue culture seedlings, the working head with two functions is arranged, so that a worker can safely take out the tissue seedlings in the culture bottle, meanwhile, the auxiliary device can assist the worker in transplanting the tissue seedlings, and damage to the tissue seedlings can be reduced under the protection of the working head.

The invention provides a mechanical arm path planning method and device for tissue culture seedling transplanting and a readable storage medium thereof.The method includes the steps that machine vision is used for recognizing the poses of tissue culture seedlings and hole tray vacancies, and a 28-dimensional state space and an 8-dimensional action space including the positions, the poses, the joint angles and the clamping force are defined; and designing a multi-subitem weighted reward function fusing successful transplantation, obstacle avoidance, attitude matching and time efficiency, and constructing a deep reinforcement learning network by adopting a soft actor-commentator (SAC) algorithm. Through virtual simulation training, a mechanical arm autonomously learns to generate a collision-free, smooth and efficient grabbing-transferring-transplanting path, and self-adaption to dynamic environments such as various tissue culture seedling forms and hole tray specification changes is achieved. According to the method, scene parameters do not need to be preset, the generalization ability and the automation level of the system are remarkably improved, and a reliable solution is provided for intelligent production of tissue culture seedling transplanting.

The present invention relates to a method for efficient regeneration of Saxifraga bronchialis, comprising the following steps: (1) material treatment; (2) induction of callus and adventitious buds; (3) proliferation of adventitious buds; (4) in-vitro rooting culture; (5) acclimatization and transplantation. The present invention uses the leaves of tissue-cultured seedlings of Saxifraga bronchialis as explants, establishes an efficient regeneration system for the leaves of Saxifraga bronchialis, determines suitable callus induction media, proliferation media, rooting media and suitable substrates for transplanting tissue-cultured seedlings. The rooted cluster buds grow well after transplantation, providing new ideas for the seedling breeding, genetic breeding of Saxifraga bronchialis and the development and utilization of plants of the genus Saxifraga.

The invention relates to the related technical field of culture instruments, in particular to a culture instrument capable of achieving automatic replacement of a culture medium. The bearing seat for placing the culture is added between the cover and the bottom of a traditional culture appliance, and the structure of the bearing seat is correspondingly optimized, so that on one hand, the sealing performance of tissue culture can be ensured; secondly, when the culture solution is replaced, the tissues do not need to be manually transplanted one by one, so that the efficiency in the application process can be greatly improved, and the problem of tissue damage in the transplanting process can be solved; thirdly, specific biological materials can be selected for subsequent experiments, and other biological materials in the culture medium are not affected; and fourthly, the purpose of realizing automatic culture can be achieved.

The present invention "a tissue culture rapid propagation method of the blue pot flower" belongs to the field of plant tissue culture rapid propagation technology, and is characterized in that it includes the following steps: S1, preparing a culture medium; the culture medium includes: MS culture medium, callus induction culture medium, callus differentiation and proliferation culture medium and rooting induction culture medium; S2, inducing callus culture; inoculating an explant sample into the callus culture medium until callus tissue is formed; S3, callus differentiation and proliferation; inoculating an explant with callus tissue into the callus differentiation and proliferation culture medium, and cultivating it to form a bud cluster; S4, inducing rooting culture; inoculating the bud cluster into the rooting induction culture, and performing rooting cultivation; S5, transplanting tissue seedlings; after the bud cluster is greater than 4 cm in height, transplanting it into a substrate, ensuring the environmental humidity, and performing normal cultivation; the substrate is a mixture of peat soil, perlite, and sand in a volume ratio of 4:3:2.

The application discloses a kind of oral cavity thread retaining pin and the method for fixing gum flap in free gingival transplantation, the retaining pin includes head;The head is connected with light pole, and the light pole is provided with threaded portion at one end, and the threaded portion is connected with tail portion;Drug delivery mechanism is arranged in the light pole, and a plurality of sustained-release micropores are arranged on the threaded portion and tail portion, and the sustained-release micropore is connected with drug delivery mechanism;The drug delivery mechanism is connected with pressing assembly, and the pressing assembly is located at the head.The method comprises the following steps: anesthesia is carried out to recipient area and donor area, recipient area is prepared, grafting tissue is obtained, and the grafting tissue is transplanted to recipient area wound surface and donor area wound surface is handled.The application can keep the close contact between gum flap and recipient bed, significantly reduce the risk of tissue flap rejection, and improve the survival rate of transplantation;Meanwhile, through the setting of pressing assembly and drug delivery mechanism, it is convenient to give medicine regularly and for a long time.

The application discloses a kind of efficient and rapid propagation method of fragrant fruit tree, belong to plant tissue culture technical field.The propagation method provided by the application includes obtaining aseptic seedling, inducing obtaining adventitious bud, proliferating adventitious bud, promoting adventitious bud elongation, promoting adventitious bud rooting and the steps such as transplanting of seedling training.The application prepares culture system including differentiation medium, proliferation medium, elongation medium and rooting medium by selecting the component of different plant hormones.The experimental results show that in the propagation method provided by the application, the differentiation rate of fragrant fruit tree adventitious bud is as high as 81.7%, the proliferation coefficient of adventitious bud is 4.7, the proportion of adventitious bud with height greater than 5mm after proliferation culture is 72.5%, the rooting rate is 77.8%, and the survival rate of transplanting tissue culture seedling to mixed substrate is 100%.The application provides an important reference for expanding the population quantity and growth speed of this precious endangered tree species fragrant fruit tree.