The invention belongs to the technical field of herbal medicinal
plant tissue culture, and specifically relates to a method for rooting out of a
bottle of
velvet antler tissue cultured seedlings, which mainly solves the problems of long rooting period in a
bottle of tissue cultured seedlings, induction of many aerial roots, and low
survival rate of
transplanting. The technical problem includes the following steps: (1) selection of
bottle seedlings; (2) cultivation of strong seedlings; (3) hardening of seedlings; (4)
transplanting of
tissue culture seedlings; (5) post-planting management. The rooting technology of grass
tissue culture seedlings outside the bottle optimizes its tissue culture and rapid propagation
system. This technology does not need rooting in a bottle, and it is directly transplanted after
hormone treatment. After two weeks, new roots can be seen from the base of the seedlings. After 30 days, the number of roots will reach 5-10, and the
root length will be 0.5-1.5cm. It will take about 2 months. The robust rooted seedlings of pilose
antler grass with a
seedling height of about 6-10 cm were obtained. Compared with the traditional rooting in a bottle, the period of
seedling cultivation is shortened by one month, and the
survival rate of
transplanting is higher than 85%. By adopting the technology of the invention, the quality of rooted seedlings is significantly improved, and industrial production can be carried out.