30results about How to "Simplify the cultivation steps" patented technology

In the invention, apical bud of zephyr lily bulb is selected for fast inducing to produce a large number adventitious buds, regeneration plant is obtained by generation and rooting of the adventitious buds, survival percentage of the regeneration plant reaches 100%; in addition, the method of the invention has the advantages of fast propagation speed, no confinement to seasons, acquisition of apical bud isolated organ at any time for tissue culture, small mutation of the regeneration plant, high genetic stability, healthy regeneration plant and shortened seedling period.

The invention belongs to the technical field of herbal medicinal plant tissue culture, and specifically relates to a method for rooting out of a bottle of velvet antler tissue cultured seedlings, which mainly solves the problems of long rooting period in a bottle of tissue cultured seedlings, induction of many aerial roots, and low survival rate of transplanting. The technical problem includes the following steps: (1) selection of bottle seedlings; (2) cultivation of strong seedlings; (3) hardening of seedlings; (4) transplanting of tissue culture seedlings; (5) post-planting management. The rooting technology of grass tissue culture seedlings outside the bottle optimizes its tissue culture and rapid propagation system. This technology does not need rooting in a bottle, and it is directly transplanted after hormone treatment. After two weeks, new roots can be seen from the base of the seedlings. After 30 days, the number of roots will reach 5-10, and the root length will be 0.5-1.5cm. It will take about 2 months. The robust rooted seedlings of pilose antler grass with a seedling height of about 6-10 cm were obtained. Compared with the traditional rooting in a bottle, the period of seedling cultivation is shortened by one month, and the survival rate of transplanting is higher than 85%. By adopting the technology of the invention, the quality of rooted seedlings is significantly improved, and industrial production can be carried out.

The invention discloses a treatment method of biodegradable post-pretreatment sucralose mother liquor waste water. High-density bacillus flora is obtained by performing separating, purifying and culturing in soil, the post-pretreatment sucralose mother liquor waste water is added and step-by-step domestication is performed, organic pollutant and ammonia nitrogen are effectively decomposed, water quality is purified, and discharge water of which indexes are standard is obtained. According to the treatment method of the biodegradable post-pretreatment sucralose mother liquor waste water, bacterium cultivation operation is simple, the condition is mild, degradation of the post-pretreatment sucralose mother liquor waste water is fast and effective, environmental protection requirements can be met well, meanwhile the cost of waste water treatment is reduced, and a new path is provided for post-pretreatment sucralose mother liquor waste water treatment in industrial production.

The invention discloses a cultivating method of strong arabidopsis seedlings. The method includes the following steps that filter paper is put in a culture dish, after the filter paper is soaked in clean water, arabidopsis seeds are put on the filter paper, and the filter paper is put in a refrigerator to be vernalized for 2-4 days at the temperature of 3-4 DEG C; the vernalized arabidopsis seedsare sown in nutrition bowls with a seedling culture medium soaked in clean water with a toothpick; 5-6 arabidopsis seeds are sown in each nutrition bowl, the nutrition bowls are covered with preservative films, the nutrition bowls are put in a stainless steel iron pan, then the stainless steel iron pan is put in a culture container to be cultured for 15-20 days, then the preservative films are removed, culture continues for 5-7 days, 4 seedlings with good growth vigor are reserved, and the other seedlings are picked off. After sowing is completed, culture is carried out for 3-4 weeks under condition of the photoperiod of 10-hour illumination and 14-hour darkness, then culture continues under the condition of the photoperiod of 16-hour illumination and 8-hour darkness, and the strong arabidopsis seedlings are obtained. Common materials are adopted, the cultivating steps of arabidopsis are simplified, the cost is reduced, the time is shortened, and the strong seedling rate of the arabidopsis is increased.

The invention relates to a tissue culture device, belonging to the field of biotechnology. The tissue culture device comprises a culture container main body and a cover, and is characterized in that a semi-solid culture medium is arranged in the lower part of the culture container main body, a stator is arranged on the upper surface of the semi-solid culture medium, and open pores are formed in the stator and used for cutting explants. The invention further provides a betula luminifera micro cuttage and rapid propagation method by combining the device. The method is simple in steps, the growing speed is high, and the occurrence of variation can be reduced. Through the method, in-vitro sterile culture can be realized, and a great amount of plants can be propagated with fewer explant materials within small range and limited culture time.

The invention relates to the biological cell technology field, particularly a method for separating and culturing miniature swine bone marrow-derived endothelial progenitor cells. The conventional EPCs culture method has the disadvantages of complexity and greatly reduced cell harvest rate by using immunomagnetic bead separation. The method in the invention comprises the steps of directly using myelomonocyte to conduct culture in vitro to obtain EPCs, adding growth factors in culture solution, planting in a definite density in a culture flask / vessel or a glass sheet enveloped by fibronectin,and conducing appropriate culture to obtain EPC. The method with high repeatability simplifies the steps of the conventional culture method, effectively avoids unnecessary cell loss caused by the conventional separation and culture method, increases the pick-up rate of EPCs, and saves a large amount of funds. The method of the invention also prepares for clinical application in future, and can reduce the usage of bone marrow and provide a technology platform for curing traumatic or ischemic diseases by autotransfusion.

In the invention, apical bud of zephyr lily bulb is selected for fast inducing to produce a large number adventitious buds, regeneration plant is obtained by generation and rooting of the adventitious buds, survival percentage of the regeneration plant reaches 100%; in addition, the method of the invention has the advantages of fast propagation speed, no confinement to seasons, acquisition of apical bud isolated organ at any time for tissue culture, small mutation of the regeneration plant, high genetic stability, healthy regeneration plant and shortened seedling period.

The invention provides a method for harvesting selenium-enriched chlorella products by a diatomite-based positively charged green flocculant, which is characterized in that it comprises: cultivating chlorella pyrenoidosa with a culture solution containing selenite, Obtain selenium-enriched cultured chlorella liquid; add diatomaceous earth-based positively charged green flocculant to the selenium-enriched cultured chlorella liquid, after standing, discard the supernatant, and harvest selenium-enriched pellets algae. In the method, a product with an organic selenium content as high as 73% can be obtained by harvesting and cultivating the selenium-enriched chlorella, wherein the cultivation step is simple, the cost is low, and the operation is convenient. In addition, the non-toxic and harmless algae flocculant is adopted, the preparation method is simple, green and pollution-free, and the recovery rate of chlorella can reach 90-97%. The method can be applied to large-scale production, and the obtained product can be applied to the fields of food health care and medical treatment, thereby creating certain economic value.

A Dendrobium officinale domesticated breeding cultivation method belongs to plant cultivation method. The method comprises the following steps: 1) disinfecting seeds produced by domesticated Dendrobium officinale with alcohol; 2) conducting induction culture on protocorm; 3) conducting differentiation culture; 4) conducting rooting culture; and 5) conducting hardening-seedling treatment, and planting in a greenhouse. The Dendrobium officinale domesticated breeding cultivation method has reasonable design; different culture media and culture conditions are employed for culture in different stages, so as to realize a final average rooting rate of 98% and an average transplanting survival rate of 97%, shorten culture cycle, and simplify culture steps.

The invention provides a medium for fast propagation of Dendrobium chinensis seedlings and a fast propagation method thereof, belonging to the technical field of plant seedling cultivation. The medium for rapid propagation of Dendrobium chinensis seeds and seedlings provided by the present invention includes the following components in mass and volume concentrations: 2.35-2.68g / L N6 basic medium, 1.0-1.5mg / L 6-BA, 0.3-0.5mg / L L 2,4-D, 30-60 g / L potato juice, 130-160 g / L banana juice, 20-30 g / L sucrose and 6-7 g / L agar; the pH value of the culture medium is 5.6-5.8. The medium for fast propagation of Dendrobium chinensis seedlings provided by the invention does not require transfer when cultivating Dendrobium chinensis seedlings, and seedlings are formed at one time, thereby avoiding pollution caused by frequent transfer, reducing the pollution rate, and greatly saving the cost of raising seedlings.

The invention relates to a method for simplifying saving of a seedless grape embryo, which is achieved by saving an in vitro embryo of the seedless grape embryo and comprises the following steps: preparing a synthetic medium, selecting and sterilizing young fruit, carrying out isolated culturing on the ovule, embryo stripping and embryo culture; selecting seed abortion seedless grape young fruit 72 days after flower blooming, washing the young fruit and sterilizing the young fruit at a clean bench respectively by corrosive sublimate and alcohol and stripping the ovule under aseptic conditions; then, inoculating the young fruit on the synthetic medium for carrying out dark culture, wherein the pH value of culture medium is 5.8-6.2 and culture temperature is 25-30 DEG C; carrying out embryostripping treatment on the ovule after the ovule is cultured for 40-60 days; and continuing to inoculate the ovule to the synthetic medium and carrying out illumination culture on the ovule for 2000-4000LX. When the illumination culture is carried out on the ovule for 20-30 days, then embryo begins to germinate, a radicle gradually grows at the lower part of the embryo and two cotyledons gradually grown at the upper part of the embryo and finally the seedling is obtained. According to the method in the invention, a growth culture medium, a germination culture medium and a seedling formation culture medium are integrated into a whole and the saving of the seedless grape embryo is simplified and accelerated.

A method for rapidly propagating ginger through tissue culture, characterized in that: the shoot tip of ginger is pretreated and placed on a slow-release medium to induce clustered buds, and then NH is added to the slow-release medium 4 NO 3 MS basal medium with half the concentration, and supplemented with 0.5~1mg / L PP 333 , continuous culture to obtain rooted shoots; the slow-release medium is supplemented with 6-BA 0.5~1.5mg / L, NAA slow-release auxin 2~3mg / L, TDZ 0.25~0.3mg / L in the MS basal medium, 2% sucrose, 0.55~0.65% agar, adjust the pH to 5.8~6. The invention simplifies the tissue culture steps, shortens the culture time, improves the survival rate and detoxification rate of sprouts, the survival rate reaches 96%, and the detoxification rate reaches 100%, and simultaneously prevents the variation and multiplication in the ginger tissue culture process. The coefficient has been significantly improved, reaching 5.13, and the rooting rate has reached 97.7%, which can cultivate robust rooted ginger seedlings.