30results about How to "Simplify the cultivation steps" patented technology

The invention discloses an immobilized nitrobacteria enrichment culture method and device in a sewage treatment process. A sequencing-batch biomembrane activated sludge process is utilized to perform enrichment culture on nitrobacteria: dewatered sludge is inoculated in an immobilized nitrobacteria culture device, and an enrichment culture solution is added; and in the nitrobacteria enrichment culture process, the biomembrane ammonia nitrogen load is gradually enhanced, and the pH value is controlled. The enrichment culture solution is formed by mixing NH4HCO3 and pretreated municipal sewage, the ammonia nitrogen concentrations are respectively 100, 300, 500, 700 and 1000 mg/L, and the COD (chemical oxygen demand) concentration is 30-60 mg/L. By controlling the immobilized nitrobacteria culture device, the DO in the reaction mixture is 2-6 mg/L, the pH value is 6.5-8.0, the biofilm-formation acclimatization culture temperature is 10-14 DEG C, and the enrichment purification culture temperature is 15-25 DEG C. The device can be used for culturing nitrobacteria at low temperature, and the nitrobacteria content is up to 40-45%. The immobilized nitrobacteria have high low-temperature nitration resistance, and can treat the wastewater of which the ammonia nitrogen concentration is up to 1000 mg/L, so that the ammonia nitrogen in the wastewater is lowered to 1.0 mg/L or below.

The invention relates to a rapid culture method for granule sludge and provides a rapid culture method for mixed culture denitrification desulphurization granule sludge. The rapid culture method provided by the invention overcomes the problems of a long period, high operation cost and poor effects of a mixed culture denitrification granule sludge process due to introduction of harmful microbes in culture of mixed culture denitrification desulphurization granule sludge in the prior art. According to the invention, since activated sludge is used as inoculation sludge and granulation of the sludge is directly realized under the condition of addition of sodium chloride, the tedious steps of culture of sulfate reduced granule sludge at first and domestication of the mixed culture denitrification desulphurization granule sludge next are omitted, culture time for the granule sludge is shortened, and operation cost is saved; moreover, usage of methane-producing sludge as the inoculation sludge and introduction of harmful microbes are prevented, and high-efficiency operation efficacy of the denitrification desulphurization granule sludge is guaranteed; meanwhile, elemental sulfur is recovered, and reclamation of wastes is realized.

The invention relates to a tissue culture device, belonging to the field of biotechnology. The tissue culture device comprises a culture container main body and a cover, and is characterized in that a semi-solid culture medium is arranged in the lower part of the culture container main body, a stator is arranged on the upper surface of the semi-solid culture medium, and open pores are formed in the stator and used for cutting explants. The invention further provides a betula luminifera micro cuttage and rapid propagation method by combining the device. The method is simple in steps, the growing speed is high, and the occurrence of variation can be reduced. Through the method, in-vitro sterile culture can be realized, and a great amount of plants can be propagated with fewer explant materials within small range and limited culture time.

The invention relates to the technical field of flower cultivation, in particular to a three-dimensional mist-spray hydroponic flower cultivation method. The purpose is to provide a method for cultivating flowers based on aerosol cultivation for rooting and adding an automatic control system on the basis of aerosol cultivation; including the following steps: (1) flower selection and cleaning; (2) flower root pruning; (3) (4) planting of flowers; (5) root-promoting treatment; (6) root growth of water roots; (7) bottling of hydroponic flowers; Less is an advantage of high survival rate, energy saving and low cost.

The invention discloses a method for quickly breeding cymbidium hybridium by use of root inducing protocorm. The improved MS culture medium containing special components (6-BA, PIC and casein hydrolysate) is used for inducing protocorm; for the root explant of cymbidium hybridium, the protocorm is induced by the root of cymbidium hybridium through a special cultivation method, wherein the induction rate reaches 100%; and after 45 days of cultivation, 50 protocorms of each root explant are obtained, the survival rate of the protocorm is 95%, and the plant grows well and directly roots.

The invention belongs to the technical field of organoid and cell culture, and particularly relates to an organoid culture system, an organoid culture method, an organoid obtained based on the organoid culture system or culture method, an application of the organoid of the organoid culture system or the organoid culture method, and an application of the organoid. The organoid culture system or culture method provided by the invention realizes organoid culture by using cells derived from a cell line, can effectively simplify the culture process of the organoid, reduces the culture cost, is suitable for culture and application of organoid models of various tissues, and provides an important research and application platform for the fields of disease model construction, drug screening, drug toxicity testing, individualized treatment and the like.

The invention discloses a method for breeding a medicinal plant valeriana jatamansi jones by means of tissue culture. The method includes steps of S1, selecting finely grown and robust wild valerianajatamansi jones, placing the wild valeriana jatamansi jones under tap water, flushing the wild valeriana jatamansi jones for more than 1 hour, then sufficiently immersing explants in 75% alcohol on anultraclean worktable in a sterile room, treating the explants for 30 s, flushing the explants by the aid of sterile water by 4-5 times, sufficiently soaking the explants in 0.1% mercury chloride solution for 10-15 min, repeatedly flushing the explants by the aid of sterile water by 5-6 times, then repeatedly shaking the explants to thoroughly clear mercury chloride, drying redundant moisture by the aid of sterile filter paper and then reserving the explants. The method for breeding the medicinal plant valeriana jatamansi jones by means of tissue culture has the advantages that the method includes simple culture steps and is easy to operate, and the medicinal plant valeriana jatamansi jones can be conveniently bred by staffs by means of tissue culture; callus tissues are induced and cultured, multiple experiments are compared to one another, accordingly, the optimal breeding conditions can be conveniently screened by users, and the requirements on industrially breeding the medicinal plant valeriana jatamansi jones by means of tissue culture can be met.

The invention discloses a triploid crucian carp tail fin cell line 3nFC which is deposited in China Center for Type Culture Collection, with preservation number CCTCC NO: C201732, the cell morphologyis typical fibroblast cells, the mode of chromosome number is as follows: 3n = 136, the number of chromosomes is correct, and cryopreservation and cell recovery can be carried out; and at the same time the cell line can be transfected and used for gene function studies, so that the foundation for immunology and virology studies for triploid crucian carp is laid, and a solid platform for n vitro studies of triploid crucian carp functional genes is laid. The invention also provides a construction method of the triploid crucian carp tail fin cell line 3nFC. In the method, during primary culture,small tail fin tissue blocks are soaked in fetal bovine serum before drying-sticking, and the soaking time is optimized, so that the small tail fin tissue can relatively well maintain the activity ofcells in the subsequent drying-sticking process after fully absorbing fetal bovine serum, and the emigration time required for the cells is greatly reduced.

The invention discloses a method for rapid propagation of ginger by tissue culture. The method is characterized by comprising the following steps of taking ginger stem tips to be pretreated, placing the pretreated ginger stem tips on a slow-release culture medium, inducing to generate cluster buds, adding an MS basal culture medium with NH4NO3 concentration halved into the slow-release culture medium, supplementing 0.5-1 mg/L of PP333, and continuously culturing to obtain rooted seedlings, wherein the slow-release culture medium is prepared by supplementing 0.5-1.5 mg/L of 6-BA, 2-3mg/L of NAA slow-release auxin, 0.25-0.3 mg/L of TDZ, 2% of cane sugar and 0.55-0.65% of agar into an MS basal culture medium, and the pH value is adjusted to 5.8-6. The tissue culture steps are simplified, the culture time is shortened, the survival rate and detoxification rate of bud seedlings are increased, the survival rate reaches 96%, the detoxification rate reaches 100%, meanwhile, variation in the ginger tissue culture process is prevented, the propagation coefficient is remarkably increased and reaches 5.13, the rooting rate reaches 97.7%, and robust ginger seedlings with roots are cultured.

The invention provides a culture medium for rapid propagation of dendrobium nobile seedlings and a rapid propagation method thereof, and belongs to the technical field of plant seedling cultivation. The culture medium for rapid propagation of dendrobium nobile seedlings is prepared from the following components in percentage by mass and volume concentration: 2.35 to 2.68 g/L of N6 basic culture medium, 1.0 to 1.5 mg/L of 6-BA, 0.3 to 0.5 mg/L of 2,4-D, 30 to 60g/L of potato juice, 130 to 160g/L of banana juice, 20 to 30g/L of cane sugar and 6 to 7g/L of agar, wherein the pH value of the culture medium is 5.6 to 5.8. When the culture medium for rapid propagation of the dendrobium nobile seedlings is used for culturing the dendrobium nobile seedlings, transferring is not needed, the seedlings are formed at a time, pollution caused by frequent transferring is avoided, the pollution rate is reduced, and the seedling culture cost is greatly saved.

The invention discloses phalaenopsis tissue culture media and preparation methods thereof. The phalaenopsis tissue culture media comprises an induction culture medium, a proliferation culture medium,a seedling strengthening culture medium and a rooting culture medium and the preparation methods comprise the preparation methods of the induction culture medium, the proliferation culture medium, theseedling strengthening culture medium and the rooting culture medium . The induction culture medium, the seedling strengthening culture medium and the rooting culture medium are obtained by adding biomass nutrients such as tomato juice, carrot puree, apple puree, banana puree and coconut juice and hormonal nutrients such as adenine, 6-benzylaminopurine and naphthalene acetic acid on the basis ofan MS medium according to nutritional and technical requirements in each culture stage. The phalaenopsis tissue culture media are applied to phalaenopsis tissue culture, have the advantages of less hormone consumption, rapid reproduction, large quantity, tidy seedling emergence, pure varieties, low cost and wide adaptability, and can improve the market competitiveness and promote development of the phalaenopsis industry.

The invention discloses a gyokuro tissue medium and a preparation method thereof. The gyokuro tissue medium comprises an induction medium, a propagation medium, a seedling strengthening medium and a rooting medium. According to the induction medium, citric acid, 6-benayl aminopurine, naphthylacetic acid, 6-furfurylaminopurine, sugar and carrageenan are added on the basis of an MS medium. Accordingto the propagation medium, citric acid, sugar and carrageenan are added on the basis of a 1/2MS medium. According to the seedling strengthening medium, citric acid, mashed potatoes, mashed apples, mashed bananas, sugar and carrageenan are added on the basis of the 1/2MS medium. According to the rooting medium, citric acid, mashed potatoes, mashed apples, mashed bananas, indolebutyric acid, activecarbon, sugar and carrageenan are added on the basis of the 1/2MS medium. The gyokuro tissue medium is used for gyokuro tissue culture, and has the advantages that the use quantity of hormones is small, propagation is rapid, the number is large, emerged seedlings are orderly, the varieties are pure, the cost is low, and the application range is wide.

The invention discloses a brain organoid model and a preparation method and application thereof, and the method comprises the following steps: obtaining pluripotent stem cells with boundary limitation and single-layer adherent growth, adding into an EB formation culture medium, and maintaining culture for 4-6 days; culturing for 2-6 days by adopting a nerve induction culture medium; and then absorbing and discarding the culture medium, coating with precooled Matrigel, curing, and sequentially replacing a neural differentiation culture medium for culturing for 3-5 days and inducing a mature culture medium for culturing for 10-30 days to obtain the brain organ-like model. According to the method, the brain organoid model is generated from single-layer cell culture for the first time, the organoid with a uniform form can be constructed in a high-throughput and one-step method, and the whole growth and development process of the organoid can be observed in situ.