30results about How to "Simplify the cultivation steps" patented technology

In the invention, apical bud of zephyr lily bulb is selected for fast inducing to produce a large number adventitious buds, regeneration plant is obtained by generation and rooting of the adventitious buds, survival percentage of the regeneration plant reaches 100%; in addition, the method of the invention has the advantages of fast propagation speed, no confinement to seasons, acquisition of apical bud isolated organ at any time for tissue culture, small mutation of the regeneration plant, high genetic stability, healthy regeneration plant and shortened seedling period.

The invention belongs to the technical field of herbal medicinal plant tissue culture, and specifically relates to a method for rooting out of a bottle of velvet antler tissue cultured seedlings, which mainly solves the problems of long rooting period in a bottle of tissue cultured seedlings, induction of many aerial roots, and low survival rate of transplanting. The technical problem includes the following steps: (1) selection of bottle seedlings; (2) cultivation of strong seedlings; (3) hardening of seedlings; (4) transplanting of tissue culture seedlings; (5) post-planting management. The rooting technology of grass tissue culture seedlings outside the bottle optimizes its tissue culture and rapid propagation system. This technology does not need rooting in a bottle, and it is directly transplanted after hormone treatment. After two weeks, new roots can be seen from the base of the seedlings. After 30 days, the number of roots will reach 5-10, and the root length will be 0.5-1.5cm. It will take about 2 months. The robust rooted seedlings of pilose antler grass with a seedling height of about 6-10 cm were obtained. Compared with the traditional rooting in a bottle, the period of seedling cultivation is shortened by one month, and the survival rate of transplanting is higher than 85%. By adopting the technology of the invention, the quality of rooted seedlings is significantly improved, and industrial production can be carried out.

The invention relates to a tissue culture device, belonging to the field of biotechnology. The tissue culture device comprises a culture container main body and a cover, and is characterized in that a semi-solid culture medium is arranged in the lower part of the culture container main body, a stator is arranged on the upper surface of the semi-solid culture medium, and open pores are formed in the stator and used for cutting explants. The invention further provides a betula luminifera micro cuttage and rapid propagation method by combining the device. The method is simple in steps, the growing speed is high, and the occurrence of variation can be reduced. Through the method, in-vitro sterile culture can be realized, and a great amount of plants can be propagated with fewer explant materials within small range and limited culture time.

The invention relates to the biological cell technology field, particularly a method for separating and culturing miniature swine bone marrow-derived endothelial progenitor cells. The conventional EPCs culture method has the disadvantages of complexity and greatly reduced cell harvest rate by using immunomagnetic bead separation. The method in the invention comprises the steps of directly using myelomonocyte to conduct culture in vitro to obtain EPCs, adding growth factors in culture solution, planting in a definite density in a culture flask / vessel or a glass sheet enveloped by fibronectin,and conducing appropriate culture to obtain EPC. The method with high repeatability simplifies the steps of the conventional culture method, effectively avoids unnecessary cell loss caused by the conventional separation and culture method, increases the pick-up rate of EPCs, and saves a large amount of funds. The method of the invention also prepares for clinical application in future, and can reduce the usage of bone marrow and provide a technology platform for curing traumatic or ischemic diseases by autotransfusion.

In the invention, apical bud of zephyr lily bulb is selected for fast inducing to produce a large number adventitious buds, regeneration plant is obtained by generation and rooting of the adventitious buds, survival percentage of the regeneration plant reaches 100%; in addition, the method of the invention has the advantages of fast propagation speed, no confinement to seasons, acquisition of apical bud isolated organ at any time for tissue culture, small mutation of the regeneration plant, high genetic stability, healthy regeneration plant and shortened seedling period.

The invention provides a method for harvesting selenium-enriched chlorella products by a diatomite-based positively charged green flocculant, which is characterized in that it comprises: cultivating chlorella pyrenoidosa with a culture solution containing selenite, Obtain selenium-enriched cultured chlorella liquid; add diatomaceous earth-based positively charged green flocculant to the selenium-enriched cultured chlorella liquid, after standing, discard the supernatant, and harvest selenium-enriched pellets algae. In the method, a product with an organic selenium content as high as 73% can be obtained by harvesting and cultivating the selenium-enriched chlorella, wherein the cultivation step is simple, the cost is low, and the operation is convenient. In addition, the non-toxic and harmless algae flocculant is adopted, the preparation method is simple, green and pollution-free, and the recovery rate of chlorella can reach 90-97%. The method can be applied to large-scale production, and the obtained product can be applied to the fields of food health care and medical treatment, thereby creating certain economic value.

A Dendrobium officinale domesticated breeding cultivation method belongs to plant cultivation method. The method comprises the following steps: 1) disinfecting seeds produced by domesticated Dendrobium officinale with alcohol; 2) conducting induction culture on protocorm; 3) conducting differentiation culture; 4) conducting rooting culture; and 5) conducting hardening-seedling treatment, and planting in a greenhouse. The Dendrobium officinale domesticated breeding cultivation method has reasonable design; different culture media and culture conditions are employed for culture in different stages, so as to realize a final average rooting rate of 98% and an average transplanting survival rate of 97%, shorten culture cycle, and simplify culture steps.

A method for rapidly propagating ginger through tissue culture, characterized in that: the shoot tip of ginger is pretreated and placed on a slow-release medium to induce clustered buds, and then NH is added to the slow-release medium 4 NO 3 MS basal medium with half the concentration, and supplemented with 0.5~1mg / L PP 333 , continuous culture to obtain rooted shoots; the slow-release medium is supplemented with 6-BA 0.5~1.5mg / L, NAA slow-release auxin 2~3mg / L, TDZ 0.25~0.3mg / L in the MS basal medium, 2% sucrose, 0.55~0.65% agar, adjust the pH to 5.8~6. The invention simplifies the tissue culture steps, shortens the culture time, improves the survival rate and detoxification rate of sprouts, the survival rate reaches 96%, and the detoxification rate reaches 100%, and simultaneously prevents the variation and multiplication in the ginger tissue culture process. The coefficient has been significantly improved, reaching 5.13, and the rooting rate has reached 97.7%, which can cultivate robust rooted ginger seedlings.