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Gyokuro tissue medium and preparation method thereof

A tissue culture medium and a culture medium technology, applied in the field of plant tissue culture, can solve the problems of increasing the reproduction coefficient, long production cycle, low reproduction rate of Yulu, and achieve the effects of less hormone dosage, low cost and lower production cost.

Inactive Publication Date: 2018-08-17
内蒙古自治区生物技术研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to overcome the problems existing in the above-mentioned prior art, provide a kind of Yulu tissue culture medium and preparation method thereof, use this medium and Yulu explant blade to carry out tissue culture, can increase reproduction coefficient greatly, solve natural The problem of low reproductive rate and long production cycle of Yulu under the conditions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: a kind of Yulu tissue culture medium, it comprises induction medium, proliferation medium, strong seedling medium and rooting medium, is characterized in that:

[0025] The induction medium comprises the following components: MS basal medium 4736mg / L, citric acid 30mg / L, 6-benzylaminopurine 3mg / L, naphthaleneacetic acid 0.1mg / L, 6-furfurylaminopurine 1mg / L, sugar 30500mg / L, carrageenan 8000mg / L;

[0026] The proliferation medium comprises the following components: 1 / 2MS basal medium 2471mg / L, citric acid 30mg / L, sugar 30500mg / L, carrageenan 8000mg / L;

[0027] The strong seedling medium comprises the following components: 1 / 2MS basal medium 2471mg / L, citric acid 30mg / L, mashed potatoes 18000mg / L, apple puree 18000mg / L, banana puree 18000mg / L, sugar 20500mg / L, Carrageenan 8000mg / L;

[0028] The rooting medium comprises the following components: 1 / 2MS medium powder 2471mg / L, citric acid 30mg / L, mashed potatoes 18000mg / L, apple puree 18000mg / L, banana puree ...

Embodiment 2

[0029] Embodiment 2: a kind of Yulu tissue culture medium, it comprises induction medium, proliferation medium, strong seedling medium and rooting medium, is characterized in that:

[0030] The induction medium comprises the following components: MS basal medium 4736mg / L, citric acid 25mg / L, 6-benzylaminopurine 4mg / L, naphthaleneacetic acid 0.05mg / L, 6-furfurylaminopurine 1.5mg / L, Sugar 29000mg / L, carrageenan 9000mg / L;

[0031] The proliferation medium comprises the following components: 1 / 2MS basal medium 2471mg / L, citric acid 25mg / L, sugar 32000mg / L, carrageenan 7000mg / L;

[0032] The strong seedling medium comprises the following components: 1 / 2MS basal medium 2471mg / L, citric acid 35mg / L, mashed potatoes 16000mg / L, apple puree 20000mg / L, banana puree 16000mg / L, sugar 19000mg / L, Carrageenan 9000mg / L;

[0033] The rooting medium comprises the following components: 1 / 2MS medium powder 2471mg / L, citric acid 35mg / L, mashed potatoes 20000mg / L, apple puree 16000mg / L, banana pur...

Embodiment 3

[0034] Embodiment 3: a kind of Yulu tissue culture medium, it comprises induction medium, proliferation medium, strong seedling medium and rooting medium, is characterized in that:

[0035] The induction medium comprises the following components: MS basal medium 4736mg / L, citric acid 35mg / L, 6-benzylaminopurine 2mg / L, naphthaleneacetic acid 0.15mg / L, 6-furfurylaminopurine 0.5mg / L, Sugar 32000mg / L, carrageenan 7000mg / L;

[0036] The proliferation medium comprises the following components: 1 / 2MS basal medium 2471mg / L, citric acid 35mg / L, sugar 29000mg / L, carrageenan 9000mg / L;

[0037] The strong seedling medium comprises the following components: 1 / 2MS basal medium 2471mg / L, citric acid 25mg / L, mashed potatoes 20000mg / L, apple puree 16000mg / L, banana puree 20000mg / L, sugar 22000mg / L, Carrageenan 7000mg / L;

[0038] The rooting medium comprises the following components: 1 / 2MS medium powder 2471mg / L, citric acid 25mg / L, mashed potatoes 16000mg / L, apple puree 20000mg / L, banana puree...

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Abstract

The invention discloses a gyokuro tissue medium and a preparation method thereof. The gyokuro tissue medium comprises an induction medium, a propagation medium, a seedling strengthening medium and a rooting medium. According to the induction medium, citric acid, 6-benayl aminopurine, naphthylacetic acid, 6-furfurylaminopurine, sugar and carrageenan are added on the basis of an MS medium. Accordingto the propagation medium, citric acid, sugar and carrageenan are added on the basis of a 1 / 2MS medium. According to the seedling strengthening medium, citric acid, mashed potatoes, mashed apples, mashed bananas, sugar and carrageenan are added on the basis of the 1 / 2MS medium. According to the rooting medium, citric acid, mashed potatoes, mashed apples, mashed bananas, indolebutyric acid, activecarbon, sugar and carrageenan are added on the basis of the 1 / 2MS medium. The gyokuro tissue medium is used for gyokuro tissue culture, and has the advantages that the use quantity of hormones is small, propagation is rapid, the number is large, emerged seedlings are orderly, the varieties are pure, the cost is low, and the application range is wide.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, in particular to a Yulu tissue culture medium and a preparation method thereof. Background technique [0002] Yulu is a soft-leaved variety of succulents in the genus Twelve Juan of the family Aphidaceae. The plant is small and exquisite, with a compact plant type and distinct layers. The leaves are plump and plump, and the leaf color is crystal clear. Strong, has a certain air purification function, caters to the modern people's concept of advocating health and pursuing beauty, and has high ornamental value and economic value. [0003] The fertility of succulents is poor, and most of them use seeding, leaf cuttings, and divisions to reproduce. However, these traditional breeding methods have the problems of low reproduction coefficient and long reproduction cycle, which are difficult to meet production needs. Therefore, the prices of famous products on the market remain high. . Co...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 杨雪峰随洋王瑞利王绎陈海军侯亚光张秀娟吴华圃赵玲玲
Owner 内蒙古自治区生物技术研究院
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