The invention provides a traditional Chinese medicine compound preparation for preventing and treating vaginitis and a preparation method thereof. The traditional Chinese medicine compound preparation comprises the following main raw materials in parts by weight: 50-70 parts of biological peptide, 15-28 parts of kuh-seng, 10-20 parts of cortex phellodendri, 10-20 parts of fructus cnidii, 5-10 parts of mint, 3-8 parts of chitosan, 5-30 parts of carbopol 940, 40-60 parts of glycerol, 2-5 parts of sodium hydroxide and 15-30 parts of mint oil. The biological peptide and traditional Chinese medicines are mixed and complementary mutually, can adjust balance, inhibit bacteria, purge fire for removing toxin, sterilize, resist inflammations, clear heat, dry dampness, dispel the wind, and kill parasites. Compared with the available preparation for preventing and treating the vaginitis, the traditional Chinese medicine compound preparation adopts a principle of mainly adjusting the balance, sterilizing and resisting the inflammations, and auxiliarily purging the fire for removing the toxin, clearing the heat, drying the dampness, dispelling the wind and killing the parasites, and is used for improving an acid environment in a vagina, inhibiting and eliminating the growth of the harmful bacteria, detoxifying, eliminating the dampness and diminishing the inflammations, so that the purpose of preventing and treating the vaginitis is achieved; and the preparation method is stable, practicable, controllable and applicable to mass production.