The invention discloses a rotating jacking mechanism and an AGV(Automatic Guided Vehicle) comprising the same and belongs to the field of automatic conveying devices. The rotating jacking mechanism comprises a base fixing plate, a rotation motor, a transmission assembly, a lead screw, a lead screw nut, a large bearing bracket, a large bearing and a hollow tray. The lead screw penetrates through the base fixing plate, and the lead screw is supported on the base fixing plate through the lead screw nut. The large bearing bracket, the large bearing and the tray are sequentially arranged on the lead screw from bottom up. The rotating motor and the transmission assembly are in transmission connection, and the transmission assembly is in transmission connection with the tray and the lead screw. The AGV comprising the rotating jacking mechanism comprises two driving wheels, four universal wheels, a battery pack, two driving motors, a driving motor driver, a vehicle body frame, two control boards, a tray support, two right-angle driving motor reduction boxes, a navigation device and two infrared sensors. According to the invention, the structure is flexible and compact, the dead weight capacity is large, the self-locking performance is good, the lead screw can ascends stably in a rotary manner and the requirements for flexible carrying can be met.