23 results about "Floor plate" patented technology

To provide floor plate members which can be stably connected to each other with a simple structure without necessity of preparing any additional members for connecting them to each other.SOLUTION: In floor plate members 100, of which each has three or more sides, of which at least one has one or more protrusions 102 or cutouts 104 shaped to fit with others, there is a rotating member 108 provided on the bottom surface of the protruding portion 102 so as to be rotatable from the top surface thereof, on the bottom surface of the cutouts 104, when the protrusions 102 and the cutouts 104 of the two floor plate members 100 are fitted together, the rotating members 108 are adjacent to each other on the inner side, there are locking members 106 provided to prevent the protrusions 102 and the cutouts 104 from moving apart in the horizontal direction, and when the rotating member 108 is rotated, the protrusions 102 and cutouts 104 are prevented from moving apart vertically.SELECTED DRAWING: Figure 1

The application provides a method and structure for reinforcing a middle partition wall under the condition of single-side filling of a double-hole air shaft, comprising: installing multiple layers of spaced distribution floor plates along the height direction of the wall; when the wall is filled with water and soil on one side, a multiple-span transverse stress one-way plate structure is formed between the wall and the multiple layers of floor plates; pouring sealing material in each hole to keep each hole sealed; locally thickening the part of the middle partition wall that cannot meet the bearing capacity requirement to form a thickened layer on the side of the wall close to the water and soil; setting a rib plate on the side of the wall away from the water and soil, one end of the rib plate being fixed on a base and the other end being fixed on a floor plate, the rib plate being not lower than the middle of the wall; anchoring the floor plate at the middle of the wall and connecting the rib plate; forming a structure on the side of the wall close to the water and soil, the structure being soil-sealed but water-unsealed; and forming a backfill layer at the bottom of the wall to strengthen the bottom constraint of the middle partition wall. The application can effectively enhance the strength of the middle partition wall and improve the reliability of the middle partition wall.

A method for assessing differentiation potential of cells in a culture broth in the differentiation of pluripotent stem cells into neural cells of the midbrain floor plate region, comprising: measuring a concentration of NT-3 in a culture supernatant in a culture broth obtained by culturing the pluripotent stem cells in a medium containing an inducer of differentiation into the neural cells of the midbrain floor plate region; comparing the measured concentration of NT-3 with a reference concentration; and when the concentration of NT-3 is equal to or more than the reference concentration, assessing the cells in the culture broth as capable of differentiating into the neural cells of the midbrain floor plate region, wherein the culture supernatant is a culture supernatant collected from the culture broth at any time from 48 hours to 240 hours after the start of culture of the pluripotent stem cells.

The present invention relates to a ground plate for leveling a crane, comprising a lower face intended to be placed on the ground and an upper face intended to receive a support plate. The ground plate is characterized in that both surface portions of the upper face and surface portions of the lower face are formed from a single folded or curved sheet of metal.

The utility model discloses an environment-friendly floor slab with a ventilation function, and relates to the technical field of environment-friendly floor slabs. In order to improve the ventilation effect, the floor comprises a first floor main body and a second floor main body, the first floor main body and the second floor main body are fixed through a connecting piece, a ventilation part is installed in the first floor main body, and the ventilation part comprises a fixing block fixed to the inner wall of the cambered surface of the first floor main body through a pin, and a first cylinder is welded to the end, away from the first floor main body, of the fixing block; and second ventilation holes are formed in the circumferential outer wall of the first barrel. In the rotating process of the device, dust attached to the surface of an insect prevention filter screen can be scraped off through a cross-shaped scraping plate, the relative position between a second cylinder body and a first cylinder body can be adjusted, and indoor and outdoor ventilation is achieved when a first ventilation hole and a second ventilation hole coincide; when the first ventilation hole and the second ventilation hole are staggered, air interception between the indoor space and the outdoor space is achieved.

To provide a small animal floor plate that can be installed in a cage or the like without using a water-permeable plate such as a punched plate as a deodorizing means for small animals made from fully ripened compost or the like. [Solution] The floor board 1 is a floor board that is placed in a cage or the like to absorb and deodorize the urine of small animals. It contains fully matured compost and diatomaceous earth and is formed into a plate shape. It is more preferable that the floor board contains humus soil in addition to the fully matured compost and diatomaceous earth. In particular, it is preferable that the humus soil contains fulvic acid and an iron complex.

To provide methods of lineage specific differentiation of pluripotent stem cells, including induced pluripotent stem cells, into floor plate midbrain progenitor cells, determined dopamine (DA) neuron progenitor cells, and / or DA neurons.SOLUTION: Provided is a method of differentiating neural cells, the method comprising: (a) performing a first incubation comprising culturing pluripotent stem cells in a non-adherent culture vessel under conditions to produce a cellular spheroid; and (b) performing a second incubation comprising culturing cells of the spheroid in a substrate-coated culture vessel under conditions to neurally differentiate the cells.SELECTED DRAWING: Figure 1

The invention relates to a floor plate (100) for a manure cellar of a stable, comprising: • - multiple passage openings (110) for the passage of animal feces, • - an embedded channel (120) having a first access opening (221) in a first side wall (104a) of the floor plate (100), and • - distributed over a length of the channel (120) two or more access locations (260) where a thickness between the channel (120) and a bottom surface of the floor plate (100) is smaller than surrounding locations.

Provided is a method for non-invasively monitoring the state of cells in a culture broth in the differentiation of pluripotent stem cells into neural cells of the midbrain floor plate region, wherein: the method for non-invasively monitoring the state of cells in a culture broth in the differentiation of pluripotent stem cells into neural cells of the midbrain floor plate region includes a step for measuring the concentration of a cell-secreted factor in the culture supernatant of a culture broth obtained by culturing pluripotent stem cells in medium that contains an agent for inducing differentiation into neural cells of the midbrain floor plate region, a step for comparing the measured cell-secreted factor concentration with a standard concentration, and a step for assessing that the cells within the culture broth can differentiate into neural cells of the midbrain floor plate region if the cell-secreted factor concentration is equal to or greater than the standard concentration; and the culture supernatant is collected from the culture broth at any time point within 336 hours after the start of culture of the pluripotent stem cells.

The present application provides a method for producing, from pluripotent stem cells, neural progenitor cells of the midbrain floor plate region or dopaminergic neural progenitor cells, the method comprising (1) inducing differentiation of pluripotent stem cells into neural progenitor cells of the midbrain floor plate region, and optionally (2) inducing differentiation of the neural progenitor cells of the midbrain floor plate region, which are obtained by the differentiation induced in (1), into dopaminergic neural progenitor cells, wherein all or part of the differentiation induced in (1) includes culturing the cells in a medium containing an agonist for LPA receptor.

The present disclosure provides methods of correcting gene variants associated with Parkinson's Disease in pluripotent stem cells, and methods of lineage specific differentiation of such corrected pluripotent stem cells into floor plate midbrain progenitor cells, determined dopamine (DA) neuron progenitor cells, and / or DA neurons, or into glial cells, such as microglial cells, astrocytes, oligodendrocytes, or ependymocytes. Also provided are compositions uses thereof, such as for treating neurodegenerative diseases and conditions, including Parkinson's disease.

The present disclosure provides methods of differentiating pluripotent stem cells, including induced pluripotent stem cells, into lineage-specific floor plate midbrain progenitor cells, determined dopaminergic neuronal progenitor cells, committed dopaminergic neuronal progenitor cells and / or dopaminergic neuronal cells. Also provided are compositions of dopaminergic neuronal progenitor cells and uses thereof, such as for treating neurodegenerative diseases and conditions, including Parkinson's disease, and articles of manufacture and kits for use thereof.

Compositions and methods are provided for the in vitro generation of functional human three-dimensional neural organizers that are functionally active and capable of choreographing in vitro midline brain development from human induced pluripotent stem cells (hiPSC). Demonstrated is a model of floor plate organizer ventral midline neurodevelopment, via the expression of a full compendium of axon guidance, morphogen, and cell signaling molecules. Floor plate organoids can be fused with spinal cord organoids into midline assembloids to induce specific cell fate and cell-cell interactions at the interface. This powerful platform can be used to model human neurodevelopment, study human genetic disorders that result from neural development, identify toxic molecules or drugs that disrupt midline brain development, and screen for therapeutics that could repair or rescue these defects.

Housing (1) for breeding animals, comprising a plurality of dividing elements (2) delimiting the standing and lying area of ​​the breeding animal, wherein the floor (3) of the housing (1) has at least one floor plate (5, 6) equipped with drainage openings (4), characterized in that a recess (7) is made in the floor (3), in which a mat (8) made of a material which is softer than the rest of the floor (3) is arranged.

A differential pressure measuring device and a differential pressure measurement method that can measure the difference between the pressure in an on-floor area and the pressure in an under-floor area even when the differential pressure is small. The device includes a differential pressure measuring unit, a first pipe of which one end is connected to a first port, and a second pipe of which one end is connected to a second port; and a cup to which the other end of the second pipe is connected and which forms a space for measuring the pressure in the under-floor area on the grating floor plate. The distance in a device height direction between the other end of the first pipe and the other end of the second pipe is fixed.

The present disclosure provides methods of lineage specific differentiation of pluripotent stem cells, including induced pluripotent stem cells, into floor plate midbrain progenitor cells, determined dopamine (DA) neuron progenitor cells, and / or DA neurons. Also provided are compositions and uses thereof, such as for treating neurodegenerative diseases and conditions, including Parkinson's disease.

A method for assessing differentiation potential of cells in a culture broth in the differentiation of pluripotent stem cells into neural cells of the midbrain floor plate region, comprising: measuring a concentration of NT-3 in a culture supernatant in a culture broth obtained by culturing the pluripotent stem cells in a medium containing an inducer of differentiation into the neural cells of the midbrain floor plate region; comparing the measured concentration of NT-3 with a reference concentration; and when the concentration of NT-3 is equal to or more than the reference concentration, assessing the cells in the culture broth as capable of differentiating into the neural cells of the midbrain floor plate region, wherein the culture supernatant is a culture supernatant collected from the culture broth at any time from 48 hours to 240 hours after the start of culture of the pluripotent stem cells.

A method for assessing differentiation potential of cells in a culture broth in the differentiation of pluripotent stem cells into neural cells of the midbrain floor plate region, comprising: measuring a concentration of NT-3 in a culture supernatant in a culture broth obtained by culturing the pluripotent stem cells in a medium containing an inducer of differentiation into the neural cells of the midbrain floor plate region; comparing the measured concentration of NT-3 with a reference concentration; and when the concentration of NT-3 is equal to or more than the reference concentration, assessing the cells in the culture broth as capable of differentiating into the neural cells of the midbrain floor plate region, wherein the culture supernatant is a culture supernatant collected from the culture broth at any time from 48 hours to 240 hours after the start of culture of the pluripotent stem cells.