99 results about "Midbrain" patented technology

The invention relates to the technical field of anesthesia management, in particular to an anesthesia medicine excess brain function influence assessment system applying artificial intelligence, which comprises a data acquisition module, a data preprocessing module, a model training module, a real-time assessment module, a risk early warning module, a treatment suggestion module, a system interaction module and a remote monitoring module. Through the combination of a convolutional neural network and a long-short-term memory network, spatial-temporal characteristics of brain wave and functional magnetic resonance imaging data are extracted, a brain function thermodynamic diagram is generated in real time, and the influence of anesthetic drugs is analyzed; the system is combined with a reinforcement learning algorithm to dynamically adjust the anesthetic dosage, a risk early warning module dynamically adjusts a threshold value through physiological parameters and tolerance of a patient, an expert team can provide medical assistance in real time through a remote monitoring module, and brain function safety of the patient in the operation or anesthesia process is ensured; according to the invention, the safety, accuracy and individuation level of anesthesia management are effectively improved.

The invention discloses a method and a related device for closed-loop optimization of a lower limb motor imagery experiment normal form through dynamic mind training, and relates to the field of rehabilitation medicines.The method comprises the steps that electroencephalogram signals of a testee when the testee executes a mind fusion lower limb motor imagery task are collected and preprocessed, and preprocessed electroencephalogram signal data are obtained; based on a dynamic feature extraction technology of a sliding window, calculating electrophysiological indexes in real time; performing weighted fusion on the motor imagery definition, the concentration degree and the correctness degree through predefined weights to generate a comprehensive state score; on the basis of the comprehensive state score, dynamically adjusting a normal feeling and task stimulation parameter applied to the testee; based on the collected electroencephalogram signals, extracting optimal perception motion rhythm and cross-frequency coupling characteristics; and inputting into the trained two-dimensional time convolution network decoding model to obtain the lower limb motor imagery intention of the testee. According to the method, the evoked rate and the stability of the electroencephalogram characteristics of the stroke patient in the lower limb motor imagery task can be improved.

The invention belongs to the technical field of brain function monitoring, and discloses a three-in-one non-invasive brain function monitoring system and method, a medium, equipment and application, and the monitoring method comprises the following steps: monitoring brain oxygen saturation of a critical patient; carrying out transcranial Doppler ultrasonic measurement on intracranial blood vessel blood flow velocity and ultrasonic measurement on the inner diameter of the optic nerve sheath; monitoring a quantitative electroencephalogram of the patient; various brain function monitoring series indexes and continuous changes of the indexes along with disease evolution are obtained, the pathogenic mechanism and pathogenesis of the indexes are found in time, and primary diseases and related complications of the primary diseases are treated. According to the invention, the brain oxygen saturation can avoid too low brain oxygen or low brain oxygen for a long time and too high brain oxygen due to oxygen utilization disorder; cerebral blood flow can be monitored through a transcranial Doppler (TCD) technology to find an optimal craniocerebral perfusion pressure (CPP), and a cerebral blood flow self-adjusting function is enabled to be in an optimal state; the electroencephalogram can avoid early warning of over-sedation or abnormal discharge and the like. Through organic combination of the three, noninvasive, real-time and dynamic monitoring of critical patients is realized.

The invention belongs to the technical field of biological medicines, and relates to application of achyranthes bidentata polypeptide combined with human umbilical cord mesenchymal stem cell exosome in preparation of a medicine for preventing and / or treating Parkinson's disease, the achyranthes bidentata polypeptide combined with the human umbilical cord mesenchymal stem cell exosome is used for treating mice, and 1-methyl-4-phenyl-1, 2, 3, 4-tetramethyl-1, 3, 4-tetramethyl-1, 3, 4-tetramethyl-1, 3, 4-tetramethyl-1, 3, 4-tetramethyl-1, 3, 4-tetramethyl-1, 3, 4 The method comprises the following steps: carrying out Parkinson model (PD) modeling on a mouse by using 2, 3, 4, 6-tetrahydropyridine (MPTP); it is found that the treatment can significantly relieve PD related symptoms, for example, the number of dopaminergic neurons in a nigra part is recovered, expression of tyrosine hydroxylase in a middle brain part is improved, movement and olfactory dysfunction of a PD model mouse is relieved, activation of olfactory bulbs and microglia and astrocytes in the middle brain part is reduced, and activity of neurons in the olfactory bulbs is improved.

The invention relates to the technical field of medical instruments, in particular to an ice stimulation training device for dysphagia rehabilitation, which comprises a box body with an upward opening, a cover plate arranged at the opening of the top of the box body, two partition plates arranged in an internal cavity at an interval up and down, and an auxiliary opening mechanism arranged in a training opening in the front side of the box body and used for opening the box body. An ice stimulation training head is arranged in the ice stimulation training device, the tail of the ice stimulation training head is connected with a control mechanism, and a pneumatic assembly is arranged on the left side of the top of the partition plate on the upper side and connected with the auxiliary opening mechanism through an air pressure rod assembly. The problems that in the prior art, an ice stimulation training device for rehabilitation of the stroke dysphagia generally can only achieve single ice stimulation treatment and lacks auxiliary taste stimulation and vibration stimulation for comprehensive treatment, so that the whole treatment process is long in time, more complex and free of cooperation, and the treatment cost is low are solved. Meanwhile, the compliance of the patient and the recognition degree of treatment are also reduced, and the patient cannot recover as soon as possible.

The present disclosure provides methods of producing midbrain dopaminergic neurons (mDAs) and precursors thereof, mDAs and precursors thereof produced thereby, as well as compositions comprising these cells, and their use for the prevention and / or treatment of neurological diseases. The present disclosure also provides methods of isolating mDA and precursors thereof from cell populations using novel surface markers.

The invention provides a method for preparing human midbrain dopaminergic nerve precursor cells. The method comprises the step of culturing human pluripotent stem cells in a culture medium with determined chemical components so as to finally obtain the human midbrain dopaminergic nerve precursor cells with expected functions. The invention also relates to functional human midbrain dopaminergic nerve precursor cells obtained by said method, as well as to compositions comprising such cells. The invention further provides application of the midbrain dopaminergic nerve precursor cell and a composition containing the midbrain dopaminergic nerve precursor cell in prevention, modeling and / or treatment of neurological disorders, and particularly, the preparation method of the human midbrain dopaminergic nerve precursor cell can be adapted to be used for preparing the human midbrain dopaminergic nerve precursor cell suitable for clinical application.

The present invention relates to a method for identifying one or more caudal midbrain (cVM) progenitor cells by measuring the presence and / or amount of adenomatous colonic polyposis down-regulating 1 (APCDD1) expressed by one or more cells. The invention also provides a method for enriching cVM cells, application of the cells in disease treatment, application of APCDD1 as a biomarker and a kit.

The present disclosure provides a method for improving in vivo survival of midbrain dopamine (mDA) neurons (e.g., in vitro differentiated mDA neurons) by suppressing p53-mediated apoptosis of the mDA neurons. The present disclosure further provides a method for treating a subject (e.g., a subject suffering from neurodegeneration and / or a neurodegenerative disease of midbrain dopamine neurons), the method comprising administering one or more mDA to the subject, whereby p53-mediated apoptosis of the one or more mDA neurons is suppressed.

The present disclosure provides methods of differentiating pluripotent stem cells, including inducible pluripotent stem cells, into lineage-specific midbrain floor progenitor cells, decisive dopaminergic neuron progenitor cells, definitive dopaminergic neuron progenitor cells, and / or dopaminergic neuron cells. Also provided are compositions use thereof, such as for the treatment of neurodegenerative diseases and conditions, including Parkinson's disease, as well as articles of manufacture and kits for use thereof.

The invention belongs to the technical field of novel application of nattokinase, and provides application of nattokinase in preparation of a medicine for preventing and / or treating Parkinson's disease. According to the invention, an MPTP / MPP + induced Parkinson's disease (PD) in-vivo and in-vitro model is established, and a neuroprotection mechanism of nattokinase is disclosed by adopting a systematic experiment method. Results show that the nattokinase can significantly improve dyskinesia and intestinal dysfunction of MPTP-induced PD mice; the damage of dopaminergic neurons of the middle brain is effectively relieved, and the density of tyrosine hydroxylase positive neurons is maintained; the expression of TLR4 and NLRP3 inflammasome pathways and related proinflammatory cytokines is inhibited, and the neuroinflammatory response is relieved; pINK1 / Parkin expression is up-regulated, mitochondrial autophagy is activated, and damaged neurons are cleared. Direct association between nattokinase and Parkinson's disease prevention and treatment is established for the first time, an innovative solution is provided for developing a novel anti-Parkinson's disease medicine with dual effects of motor function improvement and neuroprotection, and the application has important development value in the field of treatment of neurodegenerative diseases.

The application provides a midbrain organoid and a preparation method and application thereof. The midbrain organoid is obtained in vitro by using pluripotent stem cells, can simulate the development and maturation rules of nerve cells in vivo, provides a solid foundation for later researches such as building a drug screening platform and modeling of diseases related to dopaminergic neurons, and opens up a new way for cell replacement therapy.

Described herein, inter alia, are compositions and methods of use for administering a population of regulatory T (TREG) cells and / or a population of midbrain dopamine (mDA) cells to the brain of a subject (e.g., treating Parkinson's disease and / or reducing immune responses due to acupuncture wounds during cell transplantation).

A method for generating human midbrain immature neurons and mature dopaminergic neurons from neural progenitor cells is provided. The immature and mature midbrain neurons are obtained from neural progenitor cells such as committed midbrain neural stem cells (NSCs) and midbrain neural progenitor cells (midbrain NPCs), which are themselves generated from human pluripotent stem cells. The method for generating midbrain immature neurons and mature neurons uses a chemically defined medium that enables the generation of mature dopaminergic neurons in just 23 days. Media, isolated cell populations, and kits are also provided.

A method for assessing differentiation potential of cells in a culture broth in the differentiation of pluripotent stem cells into neural cells of the midbrain floor plate region, comprising: measuring a concentration of NT-3 in a culture supernatant in a culture broth obtained by culturing the pluripotent stem cells in a medium containing an inducer of differentiation into the neural cells of the midbrain floor plate region; comparing the measured concentration of NT-3 with a reference concentration; and when the concentration of NT-3 is equal to or more than the reference concentration, assessing the cells in the culture broth as capable of differentiating into the neural cells of the midbrain floor plate region, wherein the culture supernatant is a culture supernatant collected from the culture broth at any time from 48 hours to 240 hours after the start of culture of the pluripotent stem cells.

The present invention belongs to the technical field of traditional Chinese medicine, and specifically discloses a use of a traditional Chinese medicine composition in preparing a drug for preventing or treating neurodegenerative diseases. The traditional Chinese medicine composition is made of leeches, chuanxiong, salvia miltiorrhiza and astragalus, has the effects of promoting blood circulation and removing blood stasis, invigorating qi and unblocking collaterals, and can effectively improve general behavioral performance, enhance balance and coordination ability and autonomous activity ability, reduce bradykinesia symptoms, relieve anxiety, and significantly increase substantia nigra TH + The invention discloses a novel Chinese medicine composition for treating Parkinson's disease, which can reduce or delay the loss of dopaminergic neurons in the midbrain, has a neuroprotective effect, and has no liver or kidney damage. It has a significant effect on the treatment of Parkinson's disease and is a safe and effective Chinese medicine composition for the treatment of Parkinson's disease, and has a good prospect for promotion and application.

The present application provides methods for generating human midbrain immature neurons and mature dopaminergic neurons from neural progenitor cells. In some embodiments, immature and mature midbrain neurons are obtained from neural progenitor cells, such as targeted midbrain neural stem cells (NSCs) and midbrain neural progenitor cells (midbrain NPCs), while they themselves are generated from human pluripotent stem cells. Methods for generating immature and mature neurons of the midbrain use a chemically defined medium that allows for the generation of mature dopaminergic neurons within a short of 23 days. Media, isolated cell populations, and kits are also provided.

To provide methods for generating midbrain dopamine (mDA) neural progenitor cells useful for autologous cell therapy in Parkinson's disease, compositions comprising the cells, and methods of use thereof.SOLUTION: Provided is a method of producing a population of mesencephalic dopaminergic progenitor cells (mDAP), comprising the steps of providing a population of induced pluripotent stem cells (iPSC), preferably human iPSC, seeding said population of cells in discrete regions within a biological matrix hydrogel support at a density of about 5,000 to 20,000, preferably about 10,000 cells per region with a distance between said regions sufficient to maintain separation between said regions, and maintaining said cells under conditions sufficient for said iPSC to differentiate into mDAP.SELECTED DRAWING: None

The present disclosure provides methods of differentiating pluripotent stem cells, including induced pluripotent stem cells, into lineage-specific floor plate midbrain progenitor cells, determined dopaminergic neuronal progenitor cells, committed dopaminergic neuronal progenitor cells and / or dopaminergic neuronal cells. Also provided are compositions uses thereof, such as for treating neurodegenerative diseases and conditions, including Parkinson's disease, and articles of manufacture and kits for use thereof.

The invention relates to a pharmaceutical composition for intraoperative brain protection in shoulder arthroscopy surgery. The pharmaceutical composition comprises midazolam, propofol, sufentanil, succinylcholine and atracurium besylate. According to the invention, rScO2 can be improved, the risk of cerebrovascular events can be reduced, but no obvious influence is generated on the circulation stability in the operation of a patient, the postoperative cognitive impairment and the like.

The present application relates to the technical field of medical image monitoring, in particular to an intraoperative cerebral oxygen saturation image dynamic monitoring and early warning system and method for elderly patients, comprising a bilateral cerebral oxygen acquisition module, a cerebral oxygen waveform image construction module, a cerebral oxygen image feature extraction module, a hierarchical early warning module and a multi-parameter linkage display module, through the bilateral cerebral oxygen saturation time series signals output by the bilateral near-infrared cerebral oxygen saturation sensors are synthesized into a double-channel cerebral oxygen waveform image aligned with the same time window start and end time and sharing the amplitude scale, three types of geometric morphological features of bilateral cerebral oxygen slope, bilateral cerebral oxygen asymmetry index and relative baseline drop amplitude are uniformly extracted in the image domain, three types of high-risk mode hierarchical triggering of unilateral cerebral oxygen threshold persistence, relative baseline drop and bilateral asymmetry are driven, and the invasive arterial pressure, cardiac output, anesthesia depth index waveform and hemodynamic event characteristic root cause pairing display are jointly presented.

Provided is a method for differentiating pluripotent stem cells in vitro to prepare a cell population mainly composed of midbrain floor plate dopaminergic progenitor cells, wherein the progenitor cells can efficiently and directionally differentiate into midbrain dopaminergic neuron precursors and midbrain dopaminergic neurons after being transplanted into a subject. Further provided is a cell population prepared by the method, a pharmaceutical composition containing the cell population, and a method for using the cell population or the pharmaceutical composition to treat or ameliorate a neurological disorder caused by a reduction in the number of midbrain dopaminergic neurons and / or impairment of function thereof.

This method allows for early and highly accurate assessment of the likelihood of developing central nervous system diseases (especially Parkinson's disease) in the future or currently being affected. [Solution] Based on the activity level of the pontine nuclei in the brain of a living organism, disease probability information is obtained, which indicates the likelihood of developing a central nervous system disease in the future.

The application discloses an induction method for directional differentiation of neural stem cells into dopaminergic neurons, comprising the following steps: S1, activation and adhesion of NSCs; S2, directional induction of midbrain precursor cells; S3, directional induction of dopaminergic neurons; and S4, synaptic function maturation induction. The induction method for directional differentiation of neural stem cells into dopaminergic neurons can significantly improve the directional efficiency and lineage specificity of the midbrain precursor cells: by adding salvianolic acid B and icariin, and in cooperation with SHH, FGF8 and other induction factors, the midbrain lineage-specific signal pathway can be efficiently activated, the positive rate of the midbrain precursor cell marker LMX1A is increased to more than 76%, which is far better than 49% of the existing system, and abnormal differentiation of non-midbrain lineages is effectively inhibited.

The invention discloses an NDUFS2 gene heterozygous editing pig as well as a construction method and application thereof, and relates to the field of animal gene engineering and disease model construction. The eighth exon of the NDUFS2 gene of the pig is deleted or mutated, and presents an NDUFS2 heterozygous genotype; in the newborn period, the number of neurons of the middle cerebral cortical layer and the number of dopaminergic neurons are obviously reduced, the number of neurons of the adult cortical layer is continuously reduced along with mitochondrial swelling and crest fracture, and after MPTP treatment, the number of neurons of the adult cortical layer is disordered in motion trail, and the body tremor index is increased. The pig model not only can be used for revealing the action mechanism of the NDUFS2 defect in neurodegenerative diseases (such as Parkinson's disease), but also can be used as an important tool for screening new drugs, evaluating treatment means and researching mitochondrial diseases.

The present disclosure provides automated methods of differentiating pluripotent stem cells, including induced pluripotent stem cells, into lineage-specific floor plate midbrain progenitor cells, such as dopaminergic neuronal progenitor cells, determined dopaminergic neuronal progenitor cells, committed dopaminergic neuronal progenitor cells and / or dopaminergic neuronal cells. Also provided are compositions uses thereof, such as for treating neurodegenerative diseases and conditions, including Parkinson's disease, and articles of manufacture and kits for use thereof.

The present invention relates to a method for directing differentiation of ventral midbrain NSCs into neurons comprising contacting a cell population comprising ventral midbrain NSCs with an inhibitor of MEK signaling and an inhibitor of NOTCH signaling, wherein the ventral midbrain NSCs co-express the markers FOXA2, LMX1A, EN1, OTX2, 5 and SOX2.

This invention relates to methods for increasing the regeneration of a skeletal muscle that comprise increasing the concentration of Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) in the skeletal muscle. The concentration of MANF may be increased by administering to the individual MANF, a nucleic acid encoding MANF, or a cell expressing MANF. This may be useful in the treatment of skeletal muscle damage or disease, for example in individuals with an age-related reduction in skeletal muscle regenerative capacity.

The present disclosure provides methods of differentiating pluripotent stem cells, including induced pluripotent stem cells, into lineage-specific floor plate midbrain progenitor cells, determined dopaminergic neuronal progenitor cells, committed dopaminergic neuronal progenitor cells and / or dopaminergic neuronal cells. Also provided are compositions uses thereof, such as for treating neurodegenerative diseases and conditions, including Parkinson's disease, and articles of manufacture and kits for use thereof.