41 results about "Midbrain" patented technology

The invention relates to the technical field of medical instruments, in particular to an ice stimulation training device for dysphagia rehabilitation, which comprises a box body with an upward opening, a cover plate arranged at the opening of the top of the box body, two partition plates arranged in an internal cavity at an interval up and down, and an auxiliary opening mechanism arranged in a training opening in the front side of the box body and used for opening the box body. An ice stimulation training head is arranged in the ice stimulation training device, the tail of the ice stimulation training head is connected with a control mechanism, and a pneumatic assembly is arranged on the left side of the top of the partition plate on the upper side and connected with the auxiliary opening mechanism through an air pressure rod assembly. The problems that in the prior art, an ice stimulation training device for rehabilitation of the stroke dysphagia generally can only achieve single ice stimulation treatment and lacks auxiliary taste stimulation and vibration stimulation for comprehensive treatment, so that the whole treatment process is long in time, more complex and free of cooperation, and the treatment cost is low are solved. Meanwhile, the compliance of the patient and the recognition degree of treatment are also reduced, and the patient cannot recover as soon as possible.

The present disclosure provides methods of differentiating pluripotent stem cells, including inducible pluripotent stem cells, into lineage-specific midbrain floor progenitor cells, decisive dopaminergic neuron progenitor cells, definitive dopaminergic neuron progenitor cells, and / or dopaminergic neuron cells. Also provided are compositions use thereof, such as for the treatment of neurodegenerative diseases and conditions, including Parkinson's disease, as well as articles of manufacture and kits for use thereof.

The application provides a midbrain organoid and a preparation method and application thereof. The midbrain organoid is obtained in vitro by using pluripotent stem cells, can simulate the development and maturation rules of nerve cells in vivo, provides a solid foundation for later researches such as building a drug screening platform and modeling of diseases related to dopaminergic neurons, and opens up a new way for cell replacement therapy.

Described herein, inter alia, are compositions and methods of use for administering a population of regulatory T (TREG) cells and / or a population of midbrain dopamine (mDA) cells to the brain of a subject (e.g., treating Parkinson's disease and / or reducing immune responses due to acupuncture wounds during cell transplantation).

A method for assessing differentiation potential of cells in a culture broth in the differentiation of pluripotent stem cells into neural cells of the midbrain floor plate region, comprising: measuring a concentration of NT-3 in a culture supernatant in a culture broth obtained by culturing the pluripotent stem cells in a medium containing an inducer of differentiation into the neural cells of the midbrain floor plate region; comparing the measured concentration of NT-3 with a reference concentration; and when the concentration of NT-3 is equal to or more than the reference concentration, assessing the cells in the culture broth as capable of differentiating into the neural cells of the midbrain floor plate region, wherein the culture supernatant is a culture supernatant collected from the culture broth at any time from 48 hours to 240 hours after the start of culture of the pluripotent stem cells.

To provide methods for generating midbrain dopamine (mDA) neural progenitor cells useful for autologous cell therapy in Parkinson's disease, compositions comprising the cells, and methods of use thereof.SOLUTION: Provided is a method of producing a population of mesencephalic dopaminergic progenitor cells (mDAP), comprising the steps of providing a population of induced pluripotent stem cells (iPSC), preferably human iPSC, seeding said population of cells in discrete regions within a biological matrix hydrogel support at a density of about 5,000 to 20,000, preferably about 10,000 cells per region with a distance between said regions sufficient to maintain separation between said regions, and maintaining said cells under conditions sufficient for said iPSC to differentiate into mDAP.SELECTED DRAWING: None

The present disclosure provides methods of differentiating pluripotent stem cells, including induced pluripotent stem cells, into lineage-specific floor plate midbrain progenitor cells, determined dopaminergic neuronal progenitor cells, committed dopaminergic neuronal progenitor cells and / or dopaminergic neuronal cells. Also provided are compositions uses thereof, such as for treating neurodegenerative diseases and conditions, including Parkinson's disease, and articles of manufacture and kits for use thereof.

The present application relates to the technical field of medical image monitoring, in particular to an intraoperative cerebral oxygen saturation image dynamic monitoring and early warning system and method for elderly patients, comprising a bilateral cerebral oxygen acquisition module, a cerebral oxygen waveform image construction module, a cerebral oxygen image feature extraction module, a hierarchical early warning module and a multi-parameter linkage display module, through the bilateral cerebral oxygen saturation time series signals output by the bilateral near-infrared cerebral oxygen saturation sensors are synthesized into a double-channel cerebral oxygen waveform image aligned with the same time window start and end time and sharing the amplitude scale, three types of geometric morphological features of bilateral cerebral oxygen slope, bilateral cerebral oxygen asymmetry index and relative baseline drop amplitude are uniformly extracted in the image domain, three types of high-risk mode hierarchical triggering of unilateral cerebral oxygen threshold persistence, relative baseline drop and bilateral asymmetry are driven, and the invasive arterial pressure, cardiac output, anesthesia depth index waveform and hemodynamic event characteristic root cause pairing display are jointly presented.

This method allows for early and highly accurate assessment of the likelihood of developing central nervous system diseases (especially Parkinson's disease) in the future or currently being affected. [Solution] Based on the activity level of the pontine nuclei in the brain of a living organism, disease probability information is obtained, which indicates the likelihood of developing a central nervous system disease in the future.

The application discloses an induction method for directional differentiation of neural stem cells into dopaminergic neurons, comprising the following steps: S1, activation and adhesion of NSCs; S2, directional induction of midbrain precursor cells; S3, directional induction of dopaminergic neurons; and S4, synaptic function maturation induction. The induction method for directional differentiation of neural stem cells into dopaminergic neurons can significantly improve the directional efficiency and lineage specificity of the midbrain precursor cells: by adding salvianolic acid B and icariin, and in cooperation with SHH, FGF8 and other induction factors, the midbrain lineage-specific signal pathway can be efficiently activated, the positive rate of the midbrain precursor cell marker LMX1A is increased to more than 76%, which is far better than 49% of the existing system, and abnormal differentiation of non-midbrain lineages is effectively inhibited.

The invention discloses an NDUFS2 gene heterozygous editing pig as well as a construction method and application thereof, and relates to the field of animal gene engineering and disease model construction. The eighth exon of the NDUFS2 gene of the pig is deleted or mutated, and presents an NDUFS2 heterozygous genotype; in the newborn period, the number of neurons of the middle cerebral cortical layer and the number of dopaminergic neurons are obviously reduced, the number of neurons of the adult cortical layer is continuously reduced along with mitochondrial swelling and crest fracture, and after MPTP treatment, the number of neurons of the adult cortical layer is disordered in motion trail, and the body tremor index is increased. The pig model not only can be used for revealing the action mechanism of the NDUFS2 defect in neurodegenerative diseases (such as Parkinson's disease), but also can be used as an important tool for screening new drugs, evaluating treatment means and researching mitochondrial diseases.

This invention relates to methods for increasing the regeneration of a skeletal muscle that comprise increasing the concentration of Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) in the skeletal muscle. The concentration of MANF may be increased by administering to the individual MANF, a nucleic acid encoding MANF, or a cell expressing MANF. This may be useful in the treatment of skeletal muscle damage or disease, for example in individuals with an age-related reduction in skeletal muscle regenerative capacity.

This disclosure relates to a method for producing induced midbrain dopaminergic progenitor cells (imDAPs) that can provide high imDAP cell differentiation efficiency. This disclosure also provides a substantially homogeneous imDAP cell population.

The present invention relates to a method for identifying one or more caudal ventral midbrain (cVM) progenitor cells by measuring the presence and / or amount of adenomatosis polyposis coli down-regulated 1 (APCDD1) expressed by one or more cells. The present invention also provides a method for enriching cVM cells, the use of said cells in the treatment of disease, the use of APCDD1 as a biomarker, and a kit.

The invention relates to the technical field of biology, in particular to a construction method of a novel lung cancer brain transfer model. The method comprises the following steps: carrying out induction culture on stem cells to obtain an embryoid organ, and carrying out differentiation culture on the embryoid organ to obtain a midbrain ventral organ; the method comprises the following steps: shearing and digesting small cell lung cancer (SCLC) tissue blocks to obtain SCLC cells, and carrying out suspension culture to obtain small cell lung cancer (SCLC) organs; and placing the midbrain ventral organ and the small cell lung cancer organ in the same culture container, and carrying out co-culture to obtain the novel lung cancer brain transfer model. Compared with a human-derived tumor tissue xenograft technology with a low success rate, the novel lung cancer brain transfer model provided by the embodiment of the invention has high fusion efficiency and batch stability, and interference of other heterologous animal components can be reduced. In addition, the organoid fusion model established by using the technology of the invention is easier to observe, deliver drugs and sample.

The present disclosure provides methods for generating midbrain dopamine neurons and their precursors, midbrain dopamine neurons and their precursors generated by such methods, compositions comprising such cells, and uses thereof for preventing, modeling, and / or treating neurological disorders. In certain embodiments, contacting the cells with at least one inhibitor of Wnt signaling begins at least about 5 days after initial contacting of the stem cells with at least one inhibitor of SMAD signaling.

The present application provides a method for producing, from pluripotent stem cells, neural progenitor cells of the midbrain floor plate region or dopaminergic neural progenitor cells, the method comprising (1) inducing differentiation of pluripotent stem cells into neural progenitor cells of the midbrain floor plate region, and optionally (2) inducing differentiation of the neural progenitor cells of the midbrain floor plate region, which are obtained by the differentiation induced in (1), into dopaminergic neural progenitor cells, wherein all or part of the differentiation induced in (1) includes culturing the cells in a medium containing an agonist for LPA receptor.

The invention relates to the technical field of scalpels, in particular to an integrated brain glioma scalpel which comprises a scalpel rod, a driving mechanism, a reciprocating cutting mechanism, a transmission mechanism and a suction mechanism, and the scalpel rod is provided with a first cavity formed in the axial direction; the driving mechanism is arranged at one end of the cutter bar; the reciprocating cutting mechanism is arranged at the other end of the cutter bar; the transmission mechanism is connected with the driving mechanism and the reciprocating cutting mechanism through the first cavity. The suction mechanism is arranged around the reciprocating cutting mechanism and used for sucking the body fluid and discharging the body fluid through the first cavity. The problem that in the prior art, a brain glioma excision scalpel is prone to damaging normal brain tissue due to the requirement for the activity space is effectively solved.

Computing systems and computer-implemented methods for removing brain stimulation artifacts in neural signals are disclosed. The method makes use of a matching pursuit algorithm to accurately extract the stimulation artifact. The disclosed method removes the stimulation artifact associated with individual stimulation pulses without needing additional information from previous stimulation pulses or other electrodes. The disclosed method is compatible for use with various stimulation frequencies, does not need any filtering, and can recover neural signals almost immediately after stimulation. The disclosed method is compatible for use in has great potential in closed-loop systems used in various neurological diagnostic and therapeutic procedures.

The invention relates to a construction method and application of a Parkinson's disease animal model, and belongs to the technical field of animal model construction. The construction method comprises the following steps: (1) acquiring an NR4A2 flox / + mouse and a DAT cre-ERT2 mouse; (2) hybridizing the NR4A2 flox / + mouse with a DAT cre-ERT2 mouse for two times, and screening to obtain homozygous generation mice NR4A2 flox / flox and DAT cre-ERT2, wherein the NR4A2 flox / flox and the DAT cre-ERT2 are selected from the NR4A2 flox / flox and the DAT cre-ERT2; and (3) inducing the homozygous generation mice NR4A2 flox / flox and DAT cre-ERT2 by adopting the estrogen analogues, so as to obtain the Parkinson's disease model mice NR4A2 flox / flox and DAT-iCRE. The Parkinson's disease animal model which stably induces dysfunction of dopaminergic neurons of the midbrain and does not influence the survival rate of mice is successfully constructed for the first time through mouse hybridization and drug induction, and the defects that the drug stability is poor and the long disease development of PD cannot be simulated when neurotoxic drugs are injected into the abdominal cavity are effectively overcome; and the problems of poor activity, slow growth speed and low birth rate of the NR4A2 gene deletion mouse model constructed by a conventional construction method, and the limitations of difficulty in normal breeding and the like are solved.

The invention relates to the technical field of neural stem cell tests, in particular to a preparation method of a mouse midbrain tissue neural stem cell high-activity single-cell suspension. The method comprises: obtaining a mouse embryo; treating the mouse midbrain tissue to obtain a cell digestion suspension of the mouse midbrain tissue; filtering and centrifuging to obtain a mouse midbrain single-cell suspension, and performing culture and passage by using a neural stem cell culture medium to obtain P1-generation or P2-generation mouse midbrain neural spheres; resuspending the P1-generation or P2-generation mouse midbrain nerve spheres by using a culture medium, digesting the P1-generation or P2-generation mouse midbrain nerve spheres by using or not using tissue digestive enzymes, and culturing by using the following culture medium to obtain the single-cell suspension of the mouse midbrain tissue neural stem cells. According to the application, the survival rate of the single cells of the neural stem cells is ensured to be more than 95%, the cell library building requirement is met, and efficient living cells are provided for Single-cell sequencing, ATCT-Sequence sequencing and the like of the neural stem cells.

The present invention provides methods of producing, purifying and expanding mDA progenitor cells.

The present disclosure, in certain embodiments, provides methods for generating midbrain dopamine neurons and precursors thereof, midbrain dopamine neurons and precursors thereof generated by such methods and compositions comprising such cells, and uses thereof for preventing, modeling, and / or treating a neurological disorder.

The present disclosure provides methods for generating midbrain dopamine neurons (mD As) and precursors thereof, mDAs and precursors thereof generated by such methods and compositions comprising such cells, and uses thereof for preventing and / or treating neurological disorders. The present disclosure further provides methods of preventing, modeling, and / or treating at least one symptom in a subject having a neurological disorder.

Methods for generating midbrain dopamine (mDA) neuronal progenitor cells useful for autologous cell therapy in Parkinson's Disease, compositions comprising the cells, and methods of use thereof.

This invention relates to the technical field of dynamic correction of brain deformation in neurosurgical navigation, specifically to a neurosurgical navigation method and system for adaptive correction of brain deformation. The method involves acquiring preoperative MRI data to extract initial cerebrospinal fluid (CSF) level, three-dimensional domain of the whole brain, and baseline elastic modulus, and recording steady-state intracranial pressure and baseline pulse wave amplitude. Intraoperatively, real-time acquisition of head tilt angle, mean intracranial pressure, pulse wave amplitude, CSF drainage velocity, and observed cortical surface displacement is performed. Based on this, the dynamic absolute height of the CSF level and equivalent elastic modulus are calculated, a total potential energy functional with dynamic physiological boundaries and stiffness constraints is constructed, and the three-dimensional displacement field is solved using observed cortical displacement as a forced boundary. Finally, an inverse addressing strategy is used to correct the images, and navigation is restored via DICOM flow propagation. This invention eliminates the accumulated errors in deep extrapolation caused by neglecting physiological constraints in traditional static models by fusing real-time intraoperative physiological data with a variational model of continuous medium mechanics, thus achieving adaptive correction of brain deformation.

The invention discloses a three-dimensional CT-assisted accurate positioning method for intracerebral hematoma, and relates to the technical field of neurosurgery cerebral hemorrhage preoperative positioning and intraoperative drainage navigation. In the prior art, intracerebral hematoma positioning lacks a three-dimensional-body surface quantitative transfer method taking a stable anatomical baseline as a reference, and high-precision consistency of a puncture point, direction and depth is difficult to consider under a local anesthesia condition. Therefore, according to the method provided by the invention, a standard baseline layer of the same layer of eyeball crystalline lenses on the two sides and the external auditory canal is established through craniocerebral three-dimensional CT thin-layer scanning, the front-back, left-right and up-down distances of a hematoma center point relative to a connecting line and a median sagittal line of the rear edge of the external auditory canal are measured, and spatial parameters are geometrically transferred to the body surface by using a T-shaped ruler; a hematoma center body surface projection point, a mirror image point and a puncture point are determined, coronal plane and sagittal plane bidirectional guidance is formed, and individualized catheterization depth control is achieved. The method is suitable for preoperative planning and intraoperative navigation of neurosurgery intracerebral hematoma drilling drainage.

The invention provides a gene-environment interaction in-vitro model based on reward pressure stimulation as well as a construction method and application of the gene-environment interaction in-vitro model. According to the method, a human-derived midbrain organ rich in dopaminergic neurons is placed on a microelectrode array, and continuous reward pressure load is simulated by applying electrical stimulation corresponding to an explosive discharge frequency of the dopaminergic neurons, so that a chronic environmental stress condition is reconstructed. The electrical stimulation can selectively induce a patient-derived organ of a gene-environment interaction related disease to generate disease-related electrophysiology and molecular phenotypic changes, and reflects the neuroplasticity defect under the synergistic effect of gene susceptibility and environment pressure. Compared with the traditional condition that low-frequency stimulation induces long-term inhibition or no stimulation is applied, the method has unique effectiveness in the aspect of inducing the related pathological characteristics of depression. The method can be used for constructing in-vitro models of gene-environment interaction related diseases such as depression, and is suitable for drug screening and development and efficiency evaluation of diagnosis and treatment strategies.

The present disclosure provides automated methods of differentiating pluripotent stem cells, including induced pluripotent stem cells, into lineage-specific floor plate midbrain progenitor cells, such as dopaminergic neuronal progenitor cells, determined dopaminergic neuronal progenitor cells, committed dopaminergic neuronal progenitor cells and / or dopaminergic neuronal cells. Also provided are compositions uses thereof, such as for treating neurodegenerative diseases and conditions, including Parkinson's disease, and articles of manufacture and kits for use thereof.