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580 results about "Selectable marker" patented technology
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A selectable marker is a gene introduced into a cell, especially a bacterium or to cells in culture, that confers a trait suitable for artificial selection. They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or other procedure meant to introduce foreign DNA into a cell. Selectable markers are often antibiotic resistance genes (An antibiotic resistance marker is a gene that produces a protein that provides cells expressing this protein with resistance to an antibiotic.). Bacteria that have been subjected to a procedure to introduce foreign DNA are grown on a medium containing an antibiotic, and those bacterial colonies that can grow have successfully taken up and expressed the introduced genetic material. Normally the genes encoding resistance to antibiotics such as ampicillin, chloroamphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli.
Maize L3 oleosin promoter
InactiveUS6433252B1Sugar derivativesOther foreign material introduction processesAleuroneEmbryogenesis
The present invention provides methods and compositions for the identification of transgenic seeds. This is accomplished by use of screenable markers linked to aleurone-specific promoters. The screenable markers can be provided as gene fusions with selectable markers, allowing both selection and screening of transformants. The use of aleurone-specific promoters, which also direct expression in embryogenic tissues, allows efficient selection of transgenic cells and the screening of viable transgenic seeds, while avoiding the deleterious effects associated with constitutive expression of screenable marker genes. Screening of transgenic seeds avoids the need for growing and assaying of seeds for transgenes and allows implementation of automated seed screening techniques for the identification of transgenic seeds.
Owner:DEKALB GENETICS CORPORATION +1
Herbicide resistance genes
Owner:CORTEVA AGRISCIENCE LLC
Novel Herbicide Resistance Genes
The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.
Owner:CORTEVA AGRISCIENCE LLC
Herbicide resistance genes
ActiveUS8283522B2Prevent shifting or shiftingAvoid developmentBiocideSugar derivativesGlyphosateDioxygenase
The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
Owner:CORTEVA AGRISCIENCE LLC
Method of inserting/overlaying markers, data packets and objects relative to viewable content and enabling live social networking, N-dimensional virtual environments and/or other value derivable from the content
ActiveUS20080163379A1Low costReduced flexibilityDigital data processing detailsAnalogue secracy/subscription systemsNetwork packetSocial web
A method by which a User can associate selectable Markers, Data Packets and / or Objects with Content. The Content may generally be distributed electronically, and the Markers allow for insertion and / or overlay of Objects when the Content is selected for viewing by a Viewer. Objects and Data Packets are generally provided by a User, Promoter, Host, Service, or other entity to convey information to a Viewer. A Service provides tools and capabilities to both the User and the Promoter to facilitate their respective actions according to embodiments of the invention, including enabling the creation of live social networks (such as those linked to a specific Service provider, a specific User group, activities by a specific Promoter, and / or to specific Data Packets) and the creation of n-dimensional Virtual Environments.
Owner:NEONEKT LLC
System for inserting/overlaying markers, data packets and objects relative to viewable content and enabling live social networking, n-dimensional virtual environments and/or other value derivable from the content
InactiveUS20090165140A1Low costReduced flexibilityDigital data processing detailsAnalogue secracy/subscription systemsNetwork packetSocial web
A system by which a User can associate selectable Markers, Data Packets and / or Objects with Content. The Content may generally be distributed electronically, and the Markers allow for insertion and / or overlay of Objects when the Content is selected for viewing by a Viewer. Objects and Data Packets are generally provided by a User, Promoter, Host, Service, or other entity to convey information to a Viewer. A Service provides tools and capabilities to both the User and the Promoter to facilitate their respective actions according to embodiments of the invention, including enabling the creation of live social networks (such as those linked to a specific Service provider, a specific User group, activities by a specific Promoter, and / or to specific Data Packets) and the creation of n-dimensional Virtual Environments.
Owner:ADDNCLICK
Protein expression systems
InactiveUS20050186666A1High protein yieldEfficient productionBacteriaHydrolasesBiotechnologyRecombinant protein production
The present invention provides an improved expression system for the production of recombinant polypeptides utilizing auxotrophic selectable markers. In addition, the present invention provides improved recombinant protein production in host cells through the improved regulation of expression.
Owner:PFENEX
System for inserting/overlaying markers, data packets and objects relative to viewable content and enabling live social networking, N-dimensional virtual environments and/or other value derivable from the content
InactiveUS8316450B2Digital data processing detailsAnalogue secracy/subscription systemsNetwork packetService provision
Owner:ADDNCLICK
Transgenic dunaliella salina as a bioreactor
Disclosed is a method for making a bioreactor comprising a foreign target gene, special selectable markers and Dunaliella Salina as host. It is prepared by the genetic transformation techniques that include introducing a foreign target gene into the cells of Dunaliella Salina and screening the transformed cells of Dunaliella Salina. The bioreactor of the present invention can be used as a safe and cheap production system for proteins of pharmaceutical interest including vaccines, especially oral products, in a large scale, because the cells of Dunaliella Salina are easy of genetic manipulation in preparation of the bioreactor, nontoxic and edible for humans and animals, and harmless to the environment.
Owner:XUE LEXUN +1
Method for selection of transformed cells
ActiveUS20080120739A1Growth inhibitionClimate change adaptationOther foreign material introduction processesSelect agentDirect production
The invention provides methods for the selection of transgenic cells. The invention relates to the unexpected finding that cells expressing a gene conferring tolerance to auxin-like herbicides such as dicamba may be directly selected from non-transgenic cells using auxin-like herbicides as a selective agent. In this manner, plants exhibiting tolerance to auxin-like herbicides can be directly produced without the need for separate selectable markers.
Owner:MONSANTO TECH LLC
Method of forming pancreatic beta cells from mesenchymal cells
InactiveUS20050208029A1Easy to separateBiocidePancreatic cellsScreening methodBULK ACTIVE INGREDIENT
It is intended to provide a method of forming pancreatic β cells from mesenchymal cells characterized by comprising using mammal-origin mesenchymal cells as starting cells, culturing these cells in the presence of, for example, a pancreatic β cell-forming agent, and selecting and separating the thus obtained pancreatic βcells with the use of a gene expressed specifically in such cells as a selection marker; a remedy for glucose intolerance which comprises pancreatic β cells obtained by the above method as the active ingredient; a pancreatic β cell-forming agent such as a cytokine to be used in the above method; a method of screening a candidate compound promoting the formation of pancreatic β cells from mesenchymal cells; and a pancreatic β cell formation promoter obtained by this screening method.
Owner:OTSUKA PHARM CO LTD
Reagents and methods for identification of RNAi pathway genes and chemical modulators of RNAi
InactiveUS20050266552A1Increased and decreased expressionCompound screeningApoptosis detectionMammalScreening method
The present invention provides reagents such as cells, cell lines, and vectors, that can be used to identify mammalian genes whose expression products (RNA or protein) play a role in RNA interference (RNAi) and / or to identify chemical modulators of RNAi, or for other purposes. The invention further provides a variety of methods for identifying such genes or modulators. In particular, the invention provides a mammalian cell comprising a nucleic acid that encodes a selectable marker and one or more nucleic acid templates for transcription of an RNAi-inducing agent integrated into the genome of the cell, wherein the RNAi-inducing agent reduces expression of the marker and is not naturally found in the cell. Additional cells and cell lines comprising nucleic acids that encode one or more additional markers are also provided. According to certain of the inventive methods cells such as these are mutagenized, transfected or infected with a library of genetic suppressor elements, or contacted with a test compound. Cells in which RNAi is inhibited or activated are identified using an appropriate selective condition or screening method. The identity of the mutated or inhibited gene or the identity of the compound is then determined.
Owner:MASSACHUSETTS INST OF TECH
Vector capable for transformation of labyrinthulomycota
An object of the present invention is to provide a transformation system for Labyrinthulomycota that allows the elucidation of biosynthetic mechanisms of lipids such as PUFA and carotenoids as well as for the construction of a high production system and the design and development of novel functional lipid molecules by the control of the mechanisms. The present invention provides a vector for the transformation of Labyrinthulomycota with a transgene, which comprises at least (1) a nucleotide sequence which is homologous to a part of chromosomal DNA of Labyrinthulomycota and is capable of homologous recombination with the chromosomal DNA, (2) a selection marker gene having a promoter sequence located upstream and a terminator sequence located downstream, and (3) a cloning site for transgene insertion having a promoter sequence located upstream and a terminator sequence located downstream.
Owner:FUJIFILM CORP +1
Method of inserting/overlaying markers, data packets and objects relative to viewable content and enabling live social networking, N-dimensional virtual environments and/or other value derivable from the content
InactiveUS8234218B2Digital data processing detailsAnalogue secracy/subscription systemsNetwork packetSocial web
Owner:NEONEKT LLC
Expression vector, methods for the production of heterologous gene products and for the selection of recombinant cells producing high levels of such products
ActiveUS20040148647A1Shorten the timeReduce development costsAnimal cellsMicrobiological testing/measurementHeterologousHamster
An expression vector for eukaryotic cells comprising a gene which codes for a protein of interest, functionally linked to a hamster-ubiquitin / S27a-promoter and a gene which codes for a fluorescent protein. Preferably the expression vector also contains an amplifiable selectable marker gene. The invention also describes host cells, preferably mammalian cells, which have been transfected with the expression vector, processes for producing heterologous gene products and a method of selecting high-producing cells.
Owner:BOEHRINGER INGELHEIM PHARM KG
Universal chloroplast integration and expression vectors, transformed plants and products thereof
InactiveUS7129391B1Increase ratingsIncrease probabilitySugar derivativesClimate change adaptationChloroplastOrganism
The invention provides universal chloroplast integration and expression vectors which are competent to stably transform and integrate genes of interest into chloroplast genome of multiple species of plants. Transformed plants and their progeny are provided. Monocotyledonous and dicotyledonous plants are transformed which have never been transformed heretofore. Plants transformed with a synthetic gene express valuable biodegradable protein-based polymers (PBPs). Transformed plants produce high value molecules. Resistance is provided to agricultural crops against the major classes of chemical herbicides. Herbicide resistance is used as a lethal selectable marker for chloroplast transformation. The transformed plants are capable of expressing in addition to the targeted trait, a desirable, secondary non-targeted trait. Insect resistance is provided to transformed plants, both against insects that are susceptible to Bt toxins and against insects that have developed resistance to Bt toxins.
Owner:AUBURN UNIV
Retroviral gene therapy vectors and therapeutic methods based thereon
Retroviral vectors are disclosed which include an insertion site for genes of interest and are capable of expressing high levels of the protein derived from the genes of interest in a wide variety of transfected cell types. Also disclosed are retroviral vectors lacking a selectable marker, thus rendering them suitable for human gene therapy in the treatment of a variety of disease states without the co-expression of a marker product, such as an antibiotic. These retroviral vectors are especially suited for use in certain packaging cell lines.
Owner:WHITEHEAD INST FOR BIOMEDICAL RES +1
Packaging cell
A virus-producing cell sustaining the ability to produce viruses at high titer is successfully constructed by expressing the virus structural gene under the regulation of EF1α promoter. In this virus-producing cell, the virus structural gene is ligated to a selection marker gene via IRES and domains other than the protein coding domain are eliminated from the DNA encoding virus structural proteins. Thus, reduction of the titer due to cell passages can be prevented and emergence of wild type viruses caused by unfavorable recombination of the virus genome can be inhibited.
Owner:CHUGAI PHARMA CO LTD
Bacterial transglycosylases: assays for monitoring the activity using Lipid II substrate analogs and methods for discovering new antibiotics
InactiveUS6461829B1Easy to detectEasy to measureMicrobiological testing/measurementBiological material analysisEnzymatic synthesisSubstrate analog
This invention provides a direct method for monitoring bacterial transglycosylase activity using labeled substrates produced by chemo-enzymatic synthesis wherein the labels are selected to permit the detection of both polymeric and non-polymeric products simultaneously, either directly or following the separation of product from starting material. The invention promotes the discovery of new antibiotics with activity against bacterial transglycosylases by a) laying the groundwork for structural analysis of purified, active transglycosylase (which permits structure-based design); and b) providing an assay that can be used to screen for inhibitors.
Owner:THE TRUSTEES FOR PRINCETON UNIV
Transgenic microbial polyhydroxyalkanoate producers
InactiveUS6913911B2Simple production processInduce expressionSugar derivativesBacteriaBiotechnologyTransposon mutagenesis
Transgenic microbial strains are provided which contain the genes required for PHA formation integrated on the chromosome. The strains are advantageous in PHA production processes, because (1) no plasmids need to be maintained, generally obviating the required use of antibiotics or other stabilizing pressures, and (2) no plasmid loss occurs, thereby stabilizing the number of gene copies per cell throughout the fermentation process, resulting in homogeneous PHA product formation throughout the production process. Genes are integrated using standard techniques, preferably transposon mutagenesis. In a preferred embodiment wherein mutiple genes are incorporated, these are incorporated as an operon. Sequences are used to stabilize mRNA, to induce expression as a function of culture conditions (such as phosphate concentration), temperature, and stress, and to aid in selection, through the incorporation of selection markers such as markers conferring antibiotic resistance.
Owner:CJ CHEILJEDANG CORP
Novel Herbicide Resistance Genes
ActiveUS20110203017A1Prevent shifting or shiftingAvoid developmentBiocideSugar derivativesGlyphosatePyridine
The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
Owner:CORTEVA AGRISCIENCE LLC
Interleukin 15 as Selectable Marker for Gene Transfer in Lymphocytes
ActiveUS20130280221A1Opening possibilityBiocideAntibody mimetics/scaffoldsADAMTS ProteinsAntigen receptors
The present invention relates to the use of interleukin-15 (IL-15) as selectable marker for gene transfer, preferably of at least one gene of therapeutic interest, into a mammalian cell or cell line, in particular a human cell or cell line. The present invention furthermore relates to transgenic mammalian cells or cell lines expressing IL-15 as selectable marker and co-expressing at least one protein of interest encoded by at least one gene of interest, which is preferably a protein of therapeutic interest. The present invention is in particular suitable for chimeric antigen receptors (CARs) as the gene or protein of interest and their expression in lymphocytes. The transgenic mammalian cells and cell lines are furthermore suitable for use as a medicament, in particular in the treatment of cancer and in immunotherapy, such as adoptive, target-cell specific immunotherapy.
Owner:CHEMOTHERAPEUTISCHES FORSCHUNGINSTITUT GEORG SPEYER HAUS
Promotion of somatic embryogenesis in plants by PGA37 gene expression
InactiveUS7148402B2Climate change adaptationOther foreign material introduction processesApomixisSomatic embryogenesis
Owner:THE ROCKEFELLER UNIV
Novel Selectable Marker Genes
The subject invention relates to a novel gene referred to herein as DSM-2. This gene was identified in Sterptomyces coelicolor A3. The DSM-2 protein is distantly related to PAT and BAR. The subject invention also provides plant-optimized genes encoding DSM-2 proteins, DSM-2 can be used as a transgenic trait to impart tolerance in plants and plant cells to the herbicides glufosinate and bialaphos. One preferred use of the subject genes are as selectable markers. The use of this gene as a selectable marker in a bacterial system can increase efficiency for plant transformations. Use of DSM-2 as the sole selection marker eliminates the need for an additional medicinal antibiotic marker (such as ampicillin resistance) during cloning. Various other uses are also possible according to the subject invention.
Owner:CORTEVA AGRISCIENCE LLC
Method for assembling PCR fragments of DNA
InactiveUS7629120B2Simple stepsSugar derivativesMicrobiological testing/measurementOrigin of replicationSite-specific recombination
A process for assembling a series of DNA fragments generated by PCR into an ordered circular arrangement for replication and genetic work in cells. The PCR fragments are made with a modified nucleotide in the primers that can be removed with a DNA excision repair enzyme to generate a 3′ overhang. The 3′ overhangs are designed to allow directional annealing and thus sequential PCR fragments can be assembled by annealing the overhangs and subsequent ligation. Sequential addition of PCR fragments is facilitated by growing the chain on a solid support, and the assembled chain can be removed with a site specific recombinase if the first and last primers contain the recombinase site. The circularized assembled fragment can be directly used for cell transformation if the appropriate sequences are included, such as an origin of replication and a selectable marker.
Owner:RICE UNIV
Selection of host cells expressing protein at high levels
InactiveUS20060141577A1Decreases translation initiation efficiencyVectorsCell receptors/surface-antigens/surface-determinantsStart codonA-DNA
The invention provides a DNA molecule comprising a multicistronic transcription unit coding for i) a polypeptide of interest, and for ii) a selectable marker polypeptide functional in a eukaryotic host cell, wherein the polypeptide of interest has a translation initiation sequence separate from that of the selectable marker polypeptide, and wherein the coding sequence for the polypeptide of interest is upstream from the coding sequence for the selectable marker polypeptide in said multicistronic transcription unit, and wherein an internal ribosome entry site (IRES) is present downstream from the coding sequence for the polypeptide of interest and upstream from the coding sequence for the selectable marker polypeptide, and wherein the nucleic acid sequence coding for the selectable marker polypeptide in the coding strand comprises a GTG or a TTG startcodon. The invention also provides methods for obtaining host cells expressing a polypeptide of interest, said host cells comprising the DNA molecules of the invention. The invention further provides the production of polypeptides of interest, comprising culturing host cells comprising the DNA molecules according to the invention.
Owner:CHROMAGENICS BV
Constructs and methods for genome editing and genetic engineering of fungi and protists
Provided herein are constructs for genome editing or genetic engineering in fungi or protists, methods of using the constructs and media for use in selecting cells. The construct include a polynucleotide encoding a thymidine kinase operably connected to a promoter, suitably a constitutive promoter; a polynucleotide encoding an endonuclease operably connected to an inducible promoter; and a recognition site for the endonuclease. The constructs may also include selectable markers for use in selecting recombinations.
Owner:WISCONSIN ALUMNI RES FOUND
Bacteriophages for reducing toxicity of bacteria
A genetically modified bacteriophage is disclosed which comprises:(i) an exogenous polynucleotide which encodes an agent which reduces the toxicity of a bacterium; and(ii) an exogenous polynucleotide which encodes a selectable marker.Uses thereof and kits comprising same are also disclosed.
Owner:RAMOT AT TEL AVIV UNIV LTD
Methods for enhancing segregation of transgenes in plants and compositions thereof
ActiveUS7288694B2Increase nutritionIncrease productionSugar derivativesOther foreign material introduction processesSTATH genePositive selection
The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny.
Owner:MONSANTO TECH LLC
Herbicide resistance genes for resistance to aryloxyalkanoate herbicides
ActiveUS8278505B2Prevent shifting or shiftingAvoid developmentBiocideSugar derivativesGlyphosatePyridine
Owner:CORTEVA AGRISCIENCE LLC
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