55 results about "Oleosin" patented technology

The present invention provides compositions and methods for regulating expression of nucleotide sequences of interest in a plant. Compositions are novel nucleotide sequences for a seed-preferred promoter associated with the maize 17 KD OLE (17 kilodalton oleosin) coding region. A method for expressing a nucleotide sequence of interest in a plant using the regulatory sequence disclosed herein is provided.

Expression of at least one plant oleosin gene in a microbial cell engineered to produce hydrophobic / lipophilic compounds significantly increases the overall titer of the compound. The hydrophobic nature of the oleosin core is believed to increase the microbial host cell's internal storage capacity for any hydrophobic / lipophilic compound. The method is exemplified using a bacterial host cell engineered to produce significant amount of various carotenoids.

Methods for modulating oleosin expression levels in plants are provided. In particular, it relates to methods for preparing seed-derived products from seeds in which the nutrient stores of the seeds, particularly the content of seed lipids and proteins, have been altered. In particular, the present invention provides methods for the preparation of seed-derived products from seeds wherein the nutrients stored in the seeds are altered by modulating, more particularly inhibiting, oleosin gene expression.

Oleosin- and steroleosin-encoding polynucleotides from coffee plants are disclosed. Also disclosed are promoter sequences from coffee oleosin genes, and methods for using these polynucleotides and promoters for gene regulation and manipulation of flavor, aroma and other features of coffee beans.

The invention provides modified oleosins, including at least one artificially introduced cysteine, and methods and compositions for producing the modified oleosins. Also provided are polynucleotides encoding the modified oleosins, constructs and host cells comprising the polynucleotides, methods for producing oil bodies comprising the modified oleosins, in vivo and in vitro, and methods for producing oil in host cells and plants. The invention also provides methods for increasing the rate of CO2 assimilation in photosynthetic cells and plants, and involves reducing or preventing lipid recycling, and / or expressing modified oleosins with artificially introduced cysteine residues in the photosynthetic cells and plants. Also provided are methods for increasing oil production in plants, via expression of modified oleosins in the non-photosynthetic tissues / organs of plants. The method also optionally includes the step of extracting the oil from the non-photosynthetic tissues / organs of the plant, or processing the oil rich non-photosynthetic tissues / organs into animal or biofuel feedstocks.

The present invention relates to a nano-scale artificial oil body for targeted drug delivery system detection and treatment. The nano-scale artificial oil body comprises: an oleZH2 protein, wherein the oleZH2 protein comprises oleosin and a ZHer2 peptide; a lipid, a lipid-coated liposoluble drug, and a lipid-coated known fluorescence molecule, wherein the materials are prepared into an oleZH2 oil body, an oleZH2 drug oil body and an oleZH2 fluorescence oil body so as to provide an active targeted drug delivery system and a detection system.

According to the present invention, an oil body carrier is assembled through genetic engineering, and can be used in specificity detection of the oil body carrier and targeted therapy; oleosin or caleosin is combined to form an oil body vector system to carry out purification so as to rapidly and economically obtain target protein. With the present invention, an antigen oil body carrier of the oleosin or the caleosin is adopted to carry out an animal immune reaction so as to obtain a vaccine.

The invention discloses a method for expressing human osteogenic protein-1 in peanut seeds, which comprises the following steps of: utilizing a gene engineering way to connect an osteogenic protein encoding gene to an 3' terminal of an oleosin gene, constructing an 'oleosin-erepsin-human osteogenic protein' plant expression vector driven by an oleosin promoter, transforming peanuts, making recombinant protein perform high-level expression in the peanut seeds along with the accumulation of an oil body, separating and purifying the recombinant protein through floating centrifugation, and then purifying to obtain the protein after the digestion of exoproteinase. The recombinant protein obtained by the method has the advantages of safety, high activity, and easy recovery and purification, canbe stored in the peanut seeds for a long time, has convenient transportation, and can increase the added value of farm products.

The invention discloses a method for expressing human humanin protein in peanut seeds, which comprises the following steps: connecting humanin protein coding gene to a 3' end of an oleosin gene by a genetic engineering means, establishing an 'oleosin-erepsin-human humanin protein' plant expression vector driven by an oleosin promoter, transforming peanuts to realize high-level expression of recombinant protein in peanut seeds with the accumulation of oil body, then performing centrifugation of fusion protein, digesting the fusion protein by exo-protease, performing centrifugation, removing the oil phase and recovering water phase, performing purification to obtain human humanin protein. The recombinant protein obtained by the method of the invention is safe, has high activity, is very easy to be recovered and purified, can be stored in peanut seeds for a long term, is convenient for transportation, and greatly increases the crop added value.