53 results about "Oleosin" patented technology

The present invention provides methods and compositions for the identification of transgenic seeds. This is accomplished by use of screenable markers linked to aleurone-specific promoters. The screenable markers can be provided as gene fusions with selectable markers, allowing both selection and screening of transformants. The use of aleurone-specific promoters, which also direct expression in embryogenic tissues, allows efficient selection of transgenic cells and the screening of viable transgenic seeds, while avoiding the deleterious effects associated with constitutive expression of screenable marker genes. Screening of transgenic seeds avoids the need for growing and assaying of seeds for transgenes and allows implementation of automated seed screening techniques for the identification of transgenic seeds.

Expression of at least one plant oleosin gene in a microbial cell engineered to produce hydrophobic/lipophilic compounds significantly increases the overall titer of the compound. The hydrophobic nature of the oleosin core is believed to increase the microbial host cell's internal storage capacity for any hydrophobic/lipophilic compound. The method is exemplified using a bacterial host cell engineered to produce significant amount of various carotenoids.

The invention provides modified oleosins, including at least one artificially introduced cysteine, and methods and compositions for producing the modified oleosins. Also provided are polynucleotides encoding the modified oleosins, constructs and host cells comprising the polynucleotides, methods for producing oil bodies comprising the modified oleosins, in vivo and in vitro, and methods for producing oil in host cells and plants. The invention also provides methods for increasing the rate of CO₂ assimilation in photosynthetic cells and plants, and involves reducing or preventing lipid recycling, and/or expressing modified oleosins with artificially introduced cysteine residues in the photosynthetic cells and plants. Also provided are methods for increasing oil production in plants, via expression of modified oleosins in the non-photosynthetic tissues/organs of plants. The method also optionally includes the step of extrating the oil from the non-photosynthetic tissues/organs of the plant, or processing the oil rich non-photosynthetic tissues/organs into animal or biofuel feedstocks.

The invention discloses a plant expression carrier and a method for cultivating low-phytic-acid rice. The plant expression carrier comprises a hairpin structure unit inoculated into an original carrier and a specific promoter, wherein the specific promoter is a promoter Oleosin 18, and the nucleotide sequence is shown as SEQ ID NO.2. The method comprises the following steps: (1) constructing the plant expression carrier; (2) performing Agrobacterium-mediated transformation on the plant expression carrier to obtain a rice callus tissue; and (3) transferring the rice callus tissue onto a selective culture medium for further culture, transplanting onto a crop-growing field after seedling differentiation, and screening to obtain the low-phytic-acid rice plant. According to the invention, the seed specific promoter Oleosin 18 is used to control gene expression, so that gene silencing only occurs in seed embryos and aleurone layers, thereby effectively preventing the synthesis of phytic acid in other tissue positions of rice from being influenced.

A method for improving the carcass quality of an animal is described. This method invovles the preparation and use of nucleic acid fragments comprising all or substantially all of a corn oleosin promoter, a stearoyl-ACP desaturase and a delta-12 desaturase which can be used individually or in combination to modify the lipid profile of corn are described. Chimeric genes incorporating such nucleic acid fragments and suitable regulatory sequences can be used to create transgenic corn plants having altered lipid profiles are also described.

The invention belongs to the technical field of genetic engineering and specifically discloses a soybean oleosin gene GmOLEO1 as well as encoded proteins and application thereof. The gene has a nucleotide sequence shown as Seq ID NO.1. The soybean oleosin gene GmOLEO1 is obtained by cloning from soybean genome first, and the gene GmOLEO1 disclosed by the invention is introduced into plant cells by utilizing a plant expression vector, so that a transgenic plant of which the oil content is increased can be obtained. Compared with non-transgenic soybeans, soybeans in which the gene GmOLEO1 disclosed by the invention is overexpressed have the advantage that the oil content of the soybean seeds is obviously improved, and the gene GmOLEO1 plays an important role in the aspect of improving the oil content of plant seeds, particularly the soybean seeds. The achievement of the invention can be applied to regulating the soybean oleosin genes through biotechnologies, has significances for cultivating soybean varieties with high oil content and has excellent application prospects.

Methods to modulate oleosin expression levels in plants are provided. Specifically, methods for preparing seed derived products from seed, in which the composition of seed storage reserves, notably the seed lipid and protein contents, have been altered. In particular the present invention provides methods for preparing seed derived products from seed, in which the seed reserves have been altered by modulation of oleosin gene expression and more particularly the suppression of oleosin gene expression.

The invention relates to the technical field of food, and particularly relates to a method for evaluating sensitization of natural bee pollen and fermented bee pollen based on oleosin and glutaredoxin characteristic peptide indexes. According to the invention, the oleosin B2 and the glutaredoxin are used as evaluation indexes for evaluating the sensitization of the untreated natural bee pollen or the fermented bee pollen for the first time, and a specific evaluation process is provided according to the evaluation indexes for evaluating the sensitization of the untreated natural bee pollen or the fermented bee pollen. Meanwhile, on the basis of a specific protein evaluation method, the invention further provides a supplementary means for detecting the contents of oligopeptide and amino acid in the methanol extract of the bee pollen, and further verifies the evaluation result of the sensitization of the bee pollen. The evaluation method provided by the invention has the advantages of high accuracy, convenience and rapidness, and provides an evaluation basis for standardized production and quality control of bee pollen.

Oleosin- and steroleosin-encoding polynucleotides from coffee plants are disclosed. Also disclosed are promoter sequences from coffee oleosin genes, and methods for using these polynucleotides and promoters for gene regulation and manipulation of flavor, aroma and other features of coffee beans.

The invention relates to application of a soybean seed-specific promoter, belongs to the field of biotechnology, and relates to the application field of changing the seed quality and yield of cash crops. The seed-specific promoter sequence of the invention is as described in SEQ ID NO.1, and the promoter is cloned and specifically expressed in Arabidopsis thaliana seeds. Through promoter predictive analysis, about 2000bp upstream sequences of the soybean oleosin gene are isolated, and the amplified fragment and a pBI101 vector containing a GUS reporter gene are recombined. The function of Arabidopsis thaliana transgene is verified by an Agrobacterium tumefaciens inflorescence infection method, and it is proved that the seed-specific promoter could positively regulate the expression of theoleosin gene, thus providing a favorable tool for improving the seed quality and yield of crops.

The invention discloses an emulsion film and a preparation method thereof, and belongs to the technical field of food packaging materials. According to the invention, curcumin emulsion is stabilized by natural soybean oil to improve the stability of curcumin, composite natural edible biomacromolecule carboxymethyl chitosan and glycerol are used as plasticizers, and a film is prepared by a solution tape casting method, and has good mechanical properties and moisture and oxygen barrier properties; and the oil body is rich in polyunsaturated fatty acid, phytosterol, tocopherol, carotenoid, oleosin and other components beneficial to human health, curcumin also provides antioxidant and antibacterial activity, carboxymethyl chitosan has relatively strong film-forming property, biocompatibility, degradability and antibacterial activity and is non-toxic, so that the prepared film can be applied to packaging and fresh keeping of food, has the effects of delaying deterioration of products and prolonging the shelf life, and ensures the quality and safety of the food.

The invention provides a pair of specific primers for auxiliary identification of peony variety Fengdan seed fatty acid synthesis, and belongs to the field of molecular biology. The invention further provides the cDNA sequence SEQ No.1 of an oleosin gene PsOLE1 of a peony variety Fengdan, and a cloning method thereof, degenerate primers and RACE amplification primers for the cloning, real-time quantitative primers for the oleosin gene PsOLE1 of the peony variety Fengdan, and a method for auxiliary and rapid detection of the peony variety Fengdan seed fatty acid synthesis through real-time quantitative PCR with the real-time quantitative primers. According to the present invention, by using the oleosin gene, the peony variety Fengdan seed harvesting time can be guided; and compared to the traditional GC-MS technology for determining the fatty acid content, the method of the present invention has advantages of convenient and rapid detection and substantially saved determination cost so as to provide the scientific auxiliary tool for the harvesting time of the peony seed.

The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions are novel nucleotide sequences for a tissue preferred promoter isolated from the sorghum 16 kDa oleosin coding region. The sequences drive expression preferentially to seed tissue, and most preferably to embryo and/or aleurone tissue of a plant. Functional fragments of same are also provided. A method for expressing a nucleotide sequence in a plant using the regulatory sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a nucleotide sequence operably linked to one or more of the regulatory sequences of the present invention and regenerating a stably transformed plant from the transformed plant cell.