Emulsion film and preparation method thereof
An emulsion and film technology, applied in the field of emulsion film and its preparation, can solve problems such as poor moisture barrier properties, and achieve the effects of strong anti-oxidation ability, improved stability and good waterproof performance
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Embodiment 1
[0035] (1) Soak soybeans in deionized water at 4°C for 18 hours at a solid-to-liquid ratio of 1:5 (w / v). Soaked soybeans were ground in a tissue breaker for 8 minutes, wherein the solid-liquid ratio of soybeans to deionized water was 1:9 (w / v). Pass the pulverized mixture through three layers of degreasing gauze to remove the okara, and collect the filtrate. Mix sucrose and filtrate at a ratio of 1:4 (w / v) to liquid, and stir in an ice-water bath for 10 min, adjust the pH value to 7.0 with 1M NaOH, and centrifuge at 25,000×g for 30 min at 4°C to collect the rich The upper creamy layer of soybean oil bodies.
[0036] (2) In order to further purify the soybean oil bodies, dissolve the supernatant collected in step (1) in 20% (w / w) sucrose solution with a solid-liquid ratio of 1:8 (w / v), and adjust the pH value again to 7.0, then centrifuge at 25000×g for 30 min at 4°C to collect the soybean oil bodies in the upper layer, and repeat this step (2) once.
[0037] (3) Dissolve th...
Embodiment 2
[0042] (1) Soak soybeans in deionized water at 4°C for 18 hours at a solid-to-liquid ratio of 1:5 (w / v). Soaked soybeans were ground in a tissue breaker for 8 minutes, wherein the solid-liquid ratio of soybeans to deionized water was 1:9 (w / v). Pass the pulverized mixture through three layers of degreasing gauze to remove the okara, and collect the filtrate. Mix sucrose and filtrate at a ratio of 1:4 (w / v) to liquid, and stir in an ice-water bath for 10 min, adjust the pH value to 9.0 with 1M NaOH, and centrifuge at 25,000×g for 30 min at 4°C to collect the rich The upper creamy layer of soybean oil bodies.
[0043] (2) In order to further purify the soybean oil bodies, dissolve the supernatant collected in step (1) in 20% (w / w) sucrose solution with a solid-liquid ratio of 1:8 (w / v), and adjust the pH value again to 9.0, then centrifuge at 25,000×g for 30 min at 4°C to collect the soybean oil bodies in the upper layer, and repeat this step (2) once.
[0044] (3) Dissolve t...
Embodiment 3
[0049] (1) Soak soybeans in deionized water at 4°C for 18 hours at a solid-to-liquid ratio of 1:5 (w / v). Soaked soybeans were ground in a tissue breaker for 8 minutes, wherein the solid-liquid ratio of soybeans to deionized water was 1:9 (w / v). Pass the pulverized mixture through three layers of degreasing gauze to remove the okara, and collect the filtrate. Mix sucrose and filtrate at a ratio of 1:4 (w / v) to liquid, and stir in an ice-water bath for 10 min, adjust the pH value to 11.0 with 1M NaOH, and centrifuge at 25,000×g for 30 min at 4°C to collect the rich The upper creamy layer of soybean oil bodies.
[0050] (2) In order to further purify the soybean oil bodies, dissolve the supernatant collected in step (1) in 20% (w / w) sucrose solution with a solid-liquid ratio of 1:8 (w / v), and adjust the pH value again to 11.0, then centrifuge at 25000×g for 30 min at 4°C to collect the soybean oil bodies in the upper layer, and repeat this step (2) once.
[0051] (3) Dissolve ...
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