133 results about "Phytosterol" patented technology

The invention relates to the technical field of cholesterol preparation, and discloses a preparation method of plant-derived cholesterol, which comprises the following steps: 1) heating and dissolving phytosterol with n-amyl alcohol, adding stigmasterol until saturation, cooling and crystallizing, and then carrying out solid-liquid separation to obtain a stigmasterol crude product; 2) heating and dissolving the stigmasterol crude product with absolute ethyl alcohol, adding pyridine, and continuously dropwise adding acetic anhydride for reaction; after the reaction is completed, adding deionized water for mixing, extracting with n-hexane for multiple times, separating an upper-layer oil phase, and concentrating and drying the oil phase to obtain a white solid; 3, the white solid is subjected to oxidation reduction, witting reaction and hydrogenation reaction, and cholesterol is obtained.The method has the advantages that compared with the prior art that the plant source cholesterol is directly prepared from the raw material phytosterol, the process is simplified, and meanwhile the product yield can be increased.

The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to Chinese herbal medicine emulsifiable paste for dermatitis and a preparation method thereof. The traditional Chinese medicine composition is prepared from the following raw materials: radix sophorae flavescentis, cortex phellodendri, cortex dictamni, fructus cnidii, fructus kochiae, radix scutellariae, radix paeoniae rubra, radix salviae miltiorrhizae, herba schizonepetae, radix saposhnikoviae Systematic design is carried out aiming at complex pathogenesis of seborrheic dermatitis, medicinal materials such as radix sophorae flavescentis and cortex phellodendri are used for synergistically clearing heat and drying dampness, malassezia is inhibited, and lipid control and bacteriostasis are carried out from the source; radix scutellariae, radix salviae miltiorrhizae and the like are combined to cool and activate blood and control inflammation for a long time; the herba schizonepetae, the radix saposhnikoviae and the like are added for targeted itching relieving and secondary injury reduction; in the dosage form, a low-oil-burden matrix composed of light grease such as jojoba oil and squalane is adopted, the texture is light and thin, hair follicles are not blocked, and phytosterol is introduced to synchronously repair the skin barrier; according to the system, the use comfort and compliance are remarkably improved while permeation of active ingredients is guaranteed.

The application discloses a supramolecular lotus leaf peptide-narcotic alkaloid Pickering emulsion as well as a preparation method and application thereof. The water phase of the supramolecular lotus leaf peptide-narcotic alkaloid Pickering emulsion is a purified lotus leaf peptide aqueous solution, and the oil phase is camellia seed oil loaded with narcotic alkaloids. The camellia seed oil not only serves as a fat-soluble carrier to improve the solubility of the narcotic alkaloids, but also has nursing effects due to the unsaturated fatty acids, vitamin E and phytosterols contained therein. The supramolecular assembly technology is used to mediate the lotus leaf peptide and the narcotic alkaloids to form high-performance emulsified particles, to replace synthetic surfactants to stabilize the Pickering emulsion, and to construct a natural emulsion system with high emulsification efficiency, high stability and high performance, so that the performance and the nursing effect of the emulsion are optimized.

The application discloses a concentrated tomato product for assisting in reducing blood fat and a preparation method thereof. The concentrated tomato product is prepared from the following raw materials in parts by weight: mature red tomatoes 800-1000 parts, phytosterols 8-12 parts, composite dietary fibers 3-8 parts, Omega-3 fatty acid microcapsules 2-6 parts, antioxidants 0.5-1.2 parts, stabilizers 0.3-0.8 parts, and edible buffer agents in an appropriate amount. The concentrated tomato product is concentrated juice or powder. The soluble solid content of the concentrated juice is 35-40 Bx, and the moisture content of the powder product is 5.0%. The application combines phytosterols, composite dietary fibers, Omega-3 fatty acid microcapsules and other natural functional components, and the components play a synergistic effect through different blood fat reduction mechanisms, thereby comprehensively assisting in regulating lipid indexes such as cholesterol and triglyceride. The blood fat reduction effect is significantly better than that of single-component products and ordinary tomato products, and the health needs of people with high blood fat can be met.

The invention provides a rapid detection kit and a rapid detection method for phytosterol, and belongs to the field of food detection.The rapid detection kit for phytosterol comprises a phytosterol standard substance, a standard substance diluent, a substrate solution, an enzyme mixed solution, a stop solution and an elisa plate; the standard substance diluent comprises isopropanol or dimethyl sulfoxide; the substrate solution is prepared from TMB (Tetramethylbenzidine) and a substrate buffer solution; the enzyme mixed solution is a mixed solution of a cholesterol oxidase solution and a horse radish peroxidase solution. By optimizing the composition of each working reagent in the kit, the sensitivity and accuracy of phytosterol detection are remarkably improved. Meanwhile, the rapid detection method provided by the invention is simple and convenient to operate, short in detection time and suitable for on-site rapid detection.

The invention relates to the technical field of cosmetics, in particular to a preservative-free cosmetic composition for barrier repair as well as a preparation method and application of the preservative-free cosmetic composition. The invention relates to a preservative-free cosmetic composition for barrier repair, which comprises the following components in percentage by weight: 4.0-6.0% of 1, 2-pentanediol; 0.5% to 1.0% of 1, 2-hexanediol; 0.1% to 0.5% of cerayl; 0.5%-1.0% of phytosterol; 4.0%-6.0% of stearic acid; 4.0%-7.0% of hydrogenated lecithin; 0 to 0.3 percent of caryophylline; 0-0.2% of a eucommia ulmoides bark extract; and the balance of water. The composition of 1, 2-pentanediol and 1, 2-hexanediol is adopted to replace a traditional chemical preservative, and on the premise that the preservative effect is achieved, the possibility of causing skin irritation and allergy risks is reduced; ceramide and phytosterol can participate in construction of a skin barrier and supplement and repair a lipid structure of the skin barrier; stearic acid and hydrogenated lecithin form a stable emulsification basis, so that the two long-term contradictory requirements of no chemical preservative stimulation and effective barrier repair are met, and the unification of product safety and efficacy is realized.

The invention provides a pharmaceutical composition for treating prostatitis and a preparation method thereof. The pharmaceutical composition is prepared from the following raw materials in parts by weight: 20 to 60 parts of palmatine, 20 to 60 parts of paeoniflorin, 10 to 30 parts of formononetin, 20 to 60 parts of astilbin and 10 to 40 parts of stigmasterol. In-vitro cell tests and in-vivo animal experiments prove that the pharmaceutical composition has the effect of relieving the chronic prostatitis reaction.

Since it separates into at least an emulsion layer and an aqueous layer during standing, it has excellent aesthetic properties. When used, it can be uniformly redispersed by gently shaking, so it also has excellent usability. In addition, it has good storage stability, and further provides a multi-layer cosmetic excellent in the softness of the back skin. 【Means for Solving the Problem】 The following components (A) to (G): (A) Phospholipid (B) Cholesterol and / or phytosterol (C) Phosphoric acid-based surfactant (D) Oil agent (E) Sodium polyaspartate (F) Water-swelling clay mineral (G) Water A multi-layer cosmetic containing the above components.

The invention discloses a plant-based composite adhesive and a preparation method thereof, and belongs to the technical field of adhesives. The plant-based composite adhesive is prepared from peach gum essence, a composite thickener, boric acid, a composite stabilizer, absolute ethyl alcohol, deionized water and tea polyphenol. By optimizing a peach gum essence preparation process, adopting a composite thickening system of konjac glucomannan and xanthan gum and a composite stabilizing system of phytosterol and trehalose, and optimizing process parameters, the viscosity and bonding strength of the adhesive are effectively improved, the stability of the adhesive is enhanced, layering of the adhesive in the storage and use process is prevented, and the adhesive has the advantages that the adhesive is not prone to cracking, and the service life of the adhesive is prolonged. And multi-scene green requirements such as gold printing are met.

This invention discloses a soothing and repairing composition and its application, comprising the following raw materials: phytosterols, chamomile extract, hydrolyzed sodium hyaluronate, tocopherol, ceramide NP, triethanolamine, lactobacillus, macadamia seed oil, magnolia alcohol, matricaria flower extract, maltodextrin, lecithin, phosphatidylcholine, ascorbyl palmitate, inulin root juice, arginine / lysine polypeptide, squalane, jojoba seed oil, scutellaria baicalensis root extract, polygonum cuspidatum root extract, fructose, glycerin, p-hydroxyacetophenone, butylene glycol, acrylate / C10-30 alkanol acrylate crosspolymer, α-glucan oligosaccharide, 1,2-hexanediol, and water as the balance. Compared with the prior art, the soothing and repairing composition prepared by this invention has the effects of rapidly rebuilding the skin barrier, regulating skin immune function, improving skin microecology, synergistic effect, and rapid skin repair.

The invention provides an essential oil delivery system based on bionic lipidosome and a preparation method thereof, and belongs to the technical field of cosmetics and pharmaceutical preparations, a membrane material of the bionic lipidosome delivery system contains a component similar to skin cuticle lipid, and an inclusion compound formed by cyclodextrin and volatile active ingredients is encapsulated in the membrane material; the volatile active component is selected from plant essential oil or active components thereof with anti-aging, analgesic, anti-inflammatory or anti-allergy effects; the membrane material is prepared from hydrogenated soybean lecithin, cholesterol, ceramide, phytosphingosine, phytosterol and an antioxidant; according to the bionic liposome delivery system provided by the invention, the volatility of active ingredients can be remarkably reduced, the stability of the active ingredients can be improved, and slow release and synergistic infiltration promotion effects can be realized, so that the effects of the bionic liposome delivery system in anti-aging, pain relieving and other applications can be greatly enhanced.

The application belongs to the technical field of producing fat oil from raw materials, and particularly relates to a rapeseed oil extraction method with high retention of active ingredients. The rapeseed oil extraction method with high retention of active ingredients comprises the following steps: S1, performing enzyme pretreatment on rapeseed to obtain enzyme pretreated rapeseed; S2, performing subcritical extraction treatment on the enzyme pretreated rapeseed to obtain crude rapeseed oil; and S3, performing degumming treatment on the crude rapeseed oil to obtain rapeseed oil with high retention of active ingredients. Through improvement of the preparation process, the rapeseed oil with high retention of active ingredients is realized, and the content of oleic acid and phytosterol in the rapeseed oil is effectively improved.

The invention belongs to the technical field of edible plant blend oil, and discloses plant compound oil for protecting heart and blood vessels, reducing blood fat, resisting aging and fading spots and a preparation method thereof. The plant compound oil is prepared by compounding camellia oil, fermented soya beans, walnut oil, linseed oil, perilla oil, peach kernel oil, bitter apricot kernel oil, safflower seed oil and semen brassicae oil, and is prepared by the following steps: cleaning raw materials, alternately sterilizing and killing by ultraviolet rays and ozone, squeezing at low temperature, soaking the fermented soya beans at constant temperature, and stirring and mixing in vacuum. The product is rich in active ingredients such as unsaturated fatty acid, polyphenol, flavone, vitamin E, squalene and phytosterol, is high in cooking stability, has a remarkable synergistic effect of all the components, can effectively reduce triglyceride, regulate blood fat and protect heart and blood vessels, and has the effects of resisting oxidation, resisting ageing and fading spots. The process is mild, natural, safe and suitable for industrial production, and the obtained compound oil can be used as functional cooking oil and can also be used for preparing related functional food or health care products.

The invention discloses a whitening and spot-fading composition based on a fermented jasmine flower extract. The whitening and spot-fading composition comprises the following components in parts by mass: 0.1-1 part of a nano emulsifier, 0.5-8 parts of the fermented jasmine flower extract, 0.5-1.5 parts of arbutin and 1-3 parts of tranexamic acid. The nano emulsifier is a mixture of hydrogenated lecithin and phytosterol, the mass fraction ratio of arbutin to tranexamic acid is 0.5: 1, and the fermented jasmine flower extract specifically comprises a jasmine flower extract, a lactobacillus fermentation extract, water, 1, 2-hexanediol and 1, 2-pentanediol. The average particle size of the emulsion is about 256.56 nm through a nano emulsification technology, and compared with a conventional system, the transdermal absorption efficiency of active ingredients such as the jasmine flower extract, arbutin and tranexamic acid can be greatly improved, so that the active ingredients can reach action targets more easily; the problem of non-uniform dispersion caused by component solubility difference can be solved, the active component analysis risk is remarkably reduced, the system stability is improved, and the whitening and spot fading effects are enhanced.

The invention relates to the technical field of edible oil and fat processing, and discloses a sunflower seed oil double-zero double-low deodorization and by-product efficient recovery method, which comprises the following steps: carrying out degumming pretreatment on sunflower seed crude oil, pumping the sunflower seed crude oil into an extraction tower, and carrying out supercritical extraction on the sunflower seed crude oil and high-pressure carbon dioxide fluid. The extraction phase sequentially passes through a multi-stage analysis system; a refined sunflower seed oil finished product is separated by depressurizing and cooling in a first-stage analysis kettle; converting into a subcritical state in a second-stage desorption kettle, and selectively intercepting and separating out the phytosterol by utilizing beta-CD-PU porous cross-linked microspheres filled in the second-stage desorption kettle; depressurizing and separating out vitamin E in a third-stage desorption kettle; and finally entering a flash tank to gasify the carbon dioxide, and separating free fatty acid and small molecule leftovers with peculiar smell. According to the invention, nutritional ingredient loss and trans-fatty acid generation caused by high-temperature deodorization are avoided, and grease deodorization and step-by-step accurate separation and independent recovery of multiple byproducts are realized.

The invention relates to the technical field of cosmetics, and discloses an oil wax composition of a high-water-holding complex stably-loaded water active matter and a preparation method, the oil wax composition of the high-water-holding complex stably-loaded water active matter comprises a high-water-holding complex, an oil wax phase and a water-based active matter phase, the high water holding complex comprises polyglycerol-2 dipolyhydroxystearate, polyglycerol-10 eicosanoic acid ester / tetradecanedioic acid ester, polyglycerol-3 rice bran acid ester, 1%-6% of polyglycerol-6 polyricinoleate, bis-behenyl alcohol / isostearyl alcohol / phytosterol dimer linoleate, dipentaerythritol hexahydroxy stearate / hexastearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate, polyglyceryl tristearate and polyglyceryl tristearate. According to the lip care composition, a water-based active matter phase can be stably borne in a high-load mode, water-based activity is effectively conveyed to the lip surface in the using process, and therefore the moisturizing, repairing and functional care effects of the lip are remarkably improved.

This invention provides a liquid crystal biomimetic water-locking and oxygen-permeable skincare composition, its preparation method, and its application, belonging to the field of cosmetic technology. It comprises the following components by weight percentage: 20-40% glycerin, 5-10% hydrogenated lecithin, 5-10% phytosterols, 0.1-0.5% sodium stearoyl glutamate, 0.01-0.05% sodium methylstearoyl taurate, 0.1-1% ceramide NP, 1-10% caprylic / capric triglyceride, 1-10% squalane, 0.1-1% tocopheryl acetate, 0.01-0.3% sodium DNA, 0.001-0.1% fibronectin, 0.001-0.1% soluble collagen, and the balance being water. The liquid crystal biomimetic water-locking and oxygen-permeable skincare composition provided by this invention can better form a breathable protective film on the skin surface, reduce skin moisture loss, and help repair damaged skin barriers, thereby achieving the effect of water-locking and oxygen-permeable skincare.

The invention belongs to the technical field of skin care products, and particularly relates to a composition and application thereof. The invention relates to a composition, in particular to a composition with repairing and anti-wrinkle effects and application thereof. The invention provides a composition. The composition is prepared from garcinia indica seed grease, phytosterol macadamia nut oleate, a castor oil / IPDI (isophorone diisocyanate) copolymer and a saussurea involucrata extract. When the composition is used, through the synergistic effect of all the components, hydrogen bonds are connected in multiple directions, crystals are prevented from simply growing into large sheet shapes or needle shapes, and tiny, fiber-shaped or needle-shaped microcrystals tend to be formed. The microcrystals are mutually crosslinked and overlapped to construct a continuous layered network structure penetrating through the whole matrix, and excellent repairing and anti-wrinkle effects can be formed. And the components are natural, safe and mild, do not irritate the lip skin, and are suitable for long-term use.

The invention discloses application of a nutritional composition containing alpha-hydroxyisocaproic acid in weight management and blood fat improvement, and belongs to the fields of biological medicine manufacturing, biological medicine application and functional food development. The nutritional composition comprises alpha-hydroxyisocaproic acid and at least one second functional nutritional component, and the second functional nutritional component is preferably selected from one or more of the following components: 1) one or more of tea polyphenol and capsaicin; 2) one or more of beta-glucan, inulin and resistant dextrin; 3) one or more of medium chain triglyceride and sn-1, 3 diglyceride; under the condition of the same intake cycle and dosage, the nutritional composition can significantly improve the weight management effect and reduce related indexes of blood fat through a multi-way synergistic effect, and is combined with GLP-1 drugs or sequentially applied to weight management so as to reduce the risk of weight rise after drug withdrawal.

The invention relates to a quaternary lipid barrier repairing composition which is prepared from the following raw materials: tea-seed oil, camellia-seed oil, a phytosterol derivative and meadowfoam seed oil. Through compounding of the tea-seed oil, the camellia-seed oil, the phytosterol derivative and the meadowfoam seed oil, multi-dimensional barrier repair synergy of passive supplement, active repair and antioxidant protection is realized. The quaternary lipid system is highly similar to a human body sebum membrane structure, and can rapidly fill up cuticle lipid gaps, significantly reduce transdermal moisture loss and efficiently repair a skin physical barrier; the phytosterol derivative can be cooperated with the tea-seed oil and the camellia-seed oil, lipid components can be directly supplemented, a skin lipid synthesis mechanism can be activated, skin endogenous generation proportion-coordinated cholesterol-ceramide-free fatty acid is guided, and self-reconstruction of a barrier is achieved; the meadowfoam seed oil has excellent oxidation resistance, so that secondary damage of oxidative stress to lipid can be effectively resisted, and the stability and durability of repair are ensured.

The invention relates to a method of cultivating soybean exposed to at least one fungal disease comprising a step of applying to said cultivated soybean an effective treatment with a fungicide composition comprising at least fluxapyroxad in combination with at least two applications of a phytosterol composition comprising at least one phytosterol and at least one sucrose fatty acid ester to increase the yield of said cultivated soybean and / or to reduce the effects of said at least one fungal disease in comparison to soybeans under similar conditions treated only with the organo-chemical fungicide treatment.

The invention discloses a functional chewing candy base with high nutrient loading capacity and a preparation method of the functional chewing candy base, and relates to the technical field of candy preparation. Wherein the chewing sugar base is prepared from the following components in parts by weight: 300 to 600 parts of sweetening agent, 100 to 600 parts of functional nutrient, 70 to 100 parts of grease, 5 to 60 parts of polysaccharide, 2 to 12 parts of colloid and 5 to 10 parts of emulsion stabilizer. According to the invention, phytosterol is innovatively introduced into a sugar blank substrate, so that after a large number of nutrients are added, the product can still keep uniform texture and fine and smooth taste, and the texture deterioration problems of oil, stickiness and the like are effectively prevented. The texture and the taste of the chewing sugar are further optimized.

The invention relates to the technical field of aquaculture feeds, and particularly discloses a high-protein aquatic feed for improving meat quality of micropterus salmoides and a preparation method of the high-protein aquatic feed. The preparation method of the feed comprises the following steps: mixing the carnitine, the astaxanthin, the phytosterol, the tributyrin, the yucca extract, the meat structure-effect core premix, the monocalcium phosphate and the choline chloride to obtain a core mixture; extruding and puffing bran, mixing the extruded and puffed bran with the core mixture, and performing negative pressure treatment to obtain a feed core compound; and finally, carrying out glucose-chitosan-zinc lysine inner layer coating and sodium alginate gel outer layer encapsulation to prepare the high-protein aquatic feed. The activity of functional components is effectively protected through a three-stage protection system, targeted slow release of intestinal tracts is achieved, the bioavailability is remarkably improved, the growth performance of largemouth bass can be remarkably improved through the obtained feed, the feed coefficient and the abdominal fat rate are reduced, meanwhile, the muscle hardness, elasticity and collagen content are improved, and the meat quality is comprehensively improved.

The application provides a liquid chromatography-mass spectrometry method for detecting phytosterol content in dried blood spots, and belongs to the technical field of detection.The application simplifies a sampling process, uses a disposable blood taking needle and a blood taking card for sampling, replaces traditional serum or plasma samples with obtained dried blood spots, punches a sampling area of the dried blood spot sample through a puncher with a diameter of 3.2 mm, takes three dried blood spot samples, extracts seven kinds of sterol substances contained in the dried blood spot samples through n-hexane, adopts liquid chromatography-mass spectrometry, carries out detection and quantification through chromatographic separation and a mass spectrometry detector, realizes rapid and accurate determination of cholesterols, beta-sitosterols, daidzein, campesterol, dihydrocholesterol, dehydrocholesterol and 7-alkenyl cholestanol, and the method is simple in operation, accurate in quantification, and has a recovery rate of 92.4%-96.7%.

This invention discloses an ergothioneine-curcumin synergistic anti-inflammatory and anti-aging composite composition and its preparation method, relating to the fields of biomedicine and functional skincare technology. This invention constructs a ternary nanolipid carrier core-shell hierarchical structure consisting of a hydrophobic core, an interface-enhancing layer, and a hydrophilic shell. The hydrophobic core, formed by hydrogenated lecithin and phytosterols, provides a stable lipid microenvironment for curcumin. The oil-water interface region of the lipid bilayer utilizes its polar gradient to directionally anchor piperine molecules from black pepper extract, allowing piperine to be precisely positioned at the lipid membrane interface. The hydrophilic shell, composed of thiolated hyaluronic acid, covalently connects ergothioneine via thiol-disulfide bonds, stably fixing ergothioneine to the carrier surface. This allows it to exert its antioxidant protective effect upon contact with the skin or mucous membranes, while simultaneously preventing the migration and diffusion of ergothioneine into the inner lipid region. This achieves spatially ordered partitioning and functional synergy of the three active ingredients.

This invention discloses an unsaponifiable extract of olive oil, its preparation method, and its applications. The preparation method includes: S1, raw material pretreatment: after drying and pulverizing olive pulp, antioxidants and a compound enzyme are added for enzymatic hydrolysis, followed by ultrasonic cell disruption; S2, solvent extraction: extraction is performed using a hexane-anhydrous ethanol compound solvent to obtain an extract; S3, multi-stage saponification: the extract undergoes two stages of saponification sequentially, and the organic phase is collected; S4, purification and refining: after dehydration and concentration, the organic phase is dissolved in squalane and filtered to obtain a refined concentrate. This invention achieves efficient and gentle extraction of high-value components such as squalene and phytosterols through low-temperature processing, enzyme-ultrasonic synergistic cell disruption, and gradient saponification. The obtained product can be directly used in cosmetics, exhibiting excellent anti-aging and skin repair effects, and the formulation is extremely simple, gentle, and non-irritating, showing promising application prospects.

The invention relates to the technical field of fruit and vegetable preservation, in particular to phytosterol film forming liquid for fruit and vegetable preservation and a preparation method and application method. The film forming liquid is prepared from the following components in percentage by weight: 0.5 to 2.0 percent of chitosan, 0.1 to 0.5 percent of phytosterol, 0.05 to 0.3 percent of a one-dimensional nano material, 0.1 to 0.5 percent of an emulsifier, 0.5 to 1.5 percent of a plasticizer and the balance of an acid solvent; the preparation method comprises the following steps: S1, pretreatment; S2, preparation of the phytosterol film-forming liquid. According to the application method, the phytosterol film-forming liquid is applied to fruit and vegetable preservation. According to the invention, the ternary structure design of matrix-hydrophobic filler-reinforced skeleton is utilized, so that the film-forming liquid can form a compact nano composite network during film formation, the nanofiber yarns are used as a skeleton to improve the mechanical and barrier properties, and the phytosterol is used as a strong hydrophobic agent and an antioxidant to fill or cover gaps and surfaces of the network, so that the film-forming effect is improved. And the water resistance of the film is greatly improved.

The present application relates to a kind of enzyme immobilized chitosan emulsification material and its application in catalytic esterification reaction synthesis phytosterol acetate.The technical scheme used is: lipase is immobilized to chitosan surface by crosslinking agent to prepare the chitosan particle (CSPPL) of enzyme immobilized lipase, then the prepared CSPPL is used to prepare W / O Pickering emulsion catalytic system with phytosterol (PS), and the catalytic system can be used for the synthesis of phytosterol acetate, and each drop of emulsion is used as microreactor of reactant in oil phase.CSPPL can play the role of stabilizer, and also can play the role of catalyst;PS can play the role of stabilizer, and also as reaction substrate participates in catalytic reaction.Compared with homogeneous reaction, phytosterol / chitosan composite particle stable W / O Pickering emulsion catalytic system has good conversion rate.

The invention discloses a novel oil-soluble micelle preparation. The component A comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, cardiolipin, cephalin and dipalmitoyl phosphatidylcholine, and the component B comprises lecithin, soya bean lecithin, egg yolk lecithin, 1-palmitoyl-2-oleoyl lecithin, phosphatidylserine, phosphatidylinositol, phosphatidyl glycerol, phosphatidylcholine, cephalin and dipalmitoyl phosphatidylcholine; one or more of dipalmitoyl phosphatidyl ethanolamine and dipalmitoyl phosphatidyl ethanolamine; the component B comprises a component B1 and a component B2, and the component B1 comprises one or more of phytosterol, cholesterol, ergosterol, protopanoxadiol, protopanaxatriol, ganoderic acid, phytol, isophytol, retinol, andrographolide, paclitaxel, artemisinin and bisabolol; and the component B2 is prepared from one or more of 1, 2-hexanediol, ethylhexylglycerin, cyclohexyl glycerin, absolute ethyl alcohol and isosorbide dimethyl ether. The key point of the invention is to provide a low-cost formula and a low-cost preparation method for preparing the oil-soluble micelle preparation.

The invention provides an enzymatic preparation method of 3 [beta]-hydroxy-23-bisnorcholest-5-ene-22-acid methyl ester, which comprises the following steps: S1, preparing 3-oxo-23, 24-dinorcholest-4-ene-21-carboxylic acid methyl ester into an intermediate ester compound, and S2, preparing 3 [beta]-hydroxy-23-bisnorcholest-5-ene-22-acid methyl ester into 3 [beta]-hydroxy-23-bisnorcholest-5-ene-22-acid methyl ester; s2, hydrolase and ketoreductase are added into the intermediate ester obtained in the step S1, enzyme catalysis is carried out, then purification is carried out, and the 3 beta-hydroxy-23-bisnorcholest-5-ene-22-acid methyl ester is obtained through preparation. According to the enzymatic preparation method provided by the invention, a cheap and easily available phytosterol fermentation product is taken as a raw material, 3 #-site esterification is carried out through a chemical method to obtain an intermediate ester, and then a target product is finally obtained with the yield of up to 64% through one-step catalytic reaction of hydrolase and ketoreductase. The process has no loss except conversion, and the intermediate is relatively stable, so that the reaction product is relatively high in yield and good in quality, and is suitable for large-scale industrial production.