The invention discloses an operation method suitable for electrophysiological detection in cardiac cavities of rats / mice, and relates to the technical field of
experimental animal medical detection, and the operation method comprises the following steps:
anesthesia of rats / mice,
supine position fixation, and neck
skin preparation and
hair removal; separating the neck
artery of the rat / mouse, ligating the
artery blood vessel at the telecentric end, and clamping the
artery blood vessel at the proximal end by using an artery clamp; the artery
blood vessel in the middle section is temporarily retained after being punctured by an L-shaped guide needle, and the
electrophysiology catheter is inserted along the notch; slowly moving the
electrode catheter into the left
ventricle, fixing the
electrode catheter, and performing electrophysiological detection. According to the method, the detection
electrode is inserted from the neck artery, the detection electrode is sent into the
heart cavity of the rat / mouse, the integrity of the heart organ is not damaged, the risk of cardiac bleeding is avoided, accurate electrocardio data can be detected for a long time, meanwhile, after one carotid artery is
cut off, the rat / mouse can maintain basic physiological activities through the other carotid artery, and therefore the detection effect is good. Continuous electrocardio detection can be carried out so as to complete the whole set of electrocardio recording.