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165 results about "Sugar derivatives" patented technology

Quantitative detection method of polysaccharide containing uronic acid

The invention discloses a quantitative detection method of polysaccharide containing uronic acid. The method comprises the following steps: preparing a standard polysaccharide solution and a sample solution to be tested; hydrolyzing polysaccharide in the standard solution and the sample solution to be tested so as to obtain hydrolyzed residue; derivatizing the residue with 1-phenyl-3-methyl-5-pyrazolone to prepare a sugar derivative; carrying out chromatographic analysis on the sugar derivative by the utilization of a high performance liquid chromatograph and a triple quadrupole linear ion trap mass spectrometer; determining which kind of polysaccharide containing uronic acid the sample to be tested is according to the chromatogram; and preparing a standard curve of chromatographic peak area and polysaccharide concentration according to the chromatogram of the standard polysaccharide disaccharide derivative obtained by high performance liquid chromatography-mass spectrum, and calculating content of polysaccharide containing uronic acid in the sample to be tested by the utilization of peak area of the sample to be tested. According to the method provided by the invention, quantitative detection of polysaccharides can be accurately carried out, and effective means is provided for quality control of products such as medicines, health product and the like of the polysaccharides.
Owner:DALIAN POLYTECHNIC UNIVERSITY
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