The invention discloses a preparation method of a novel vegetable and fruit fermented beverage. The preparation method comprises the steps of: firstly carrying out enzymolysis on mixed fresh juice of fresh vegetables and fruits after
dilution and size mixing by adopting
cellulase, fermenting by adopting a mixed
lactic acid starter after adding
sugar and corn
oligopeptide as
fermentation substrates and sterilizing, adding
calcium carbonate when the pH reaches 4.0, and then inoculating
leuconostoc mesenteroides for
continuous fermentation; adding a feed supplement and sterilizing when the pH reaches 3.5, and then adding high-activity dry
yeast for the
fermentation for 2-3 days; and finally performing sterilization,
centrifugation, homogenizing, blending, sterilizing and filling to obtain the novel vegetable and fruit fermented functional beverage. The enzymolysis is firstly carried out on a product by the
cellulase, then
lactic acid bacteria and
saccharomycetes are utilized for the step-by-step
fermentation of the mixed fresh juice of the fresh vegetables and fruits, and the corn
oligopeptide is added to promote the growth and the
metabolism of fermentation strains, so that the novel vegetable and fruit fermented beverage has clear composition of functional components, contains abundant
oligopeptide, organic acids, reducing sugars,
sugar alcohols and
soluble dietary fiber, and integrates
nutrition and health care, thus being a novel vegetable and fruit fermented beverage.