144 results about "Microbial contamination" patented technology

The invention discloses a system and method for identifying microbial contamination in food and cosmetics based on AI identification, and the method comprises the steps: collecting to-be-detected food or cosmetics as a detection sample, and obtaining the bacterial colony growth state of microorganisms in the detection sample; the method comprises the following steps: acquiring a microbial growth image through microscopic imaging equipment, and preprocessing the microbial growth image to obtain a target area of a microbial colony; constructing an AI recognition model by adopting a convolutional neural network, and obtaining the species and quantity of microorganisms in the detection sample; parameters of the AI recognition model are adjusted in real time according to the types and the number of the microorganisms, a preset microorganism limited standard threshold value is combined, a visual analysis report is generated, the AI recognition model is combined with a Faster R-CNN target detection method, precise classification and number statistics of the types of the microorganisms are achieved, a complete microorganism pollution analysis report is formed, and the method is suitable for popularization and application. And the accuracy and scientificity of the final identification result are improved.

The invention relates to a processing method of fermented edible sheepskin based on plant lactobacillus, and belongs to the technical field of food processing. Aiming at the problems of fiber damage, high microbial pollution risk, texture hardening after marinating and the like caused by chemical treatment in the traditional sheepskin processing, the invention provides a solution of combining biological fermentation with gradient temperature control. The method is characterized by comprising the following steps: soaking sheepskin (subjected to unhairing treatment) in a 4-6% sodium carbonate solution (40 DEG C, 2 hours) for pretreatment; activating the plant lactobacillus until the viable bacterium concentration is 8 * 8 * 10 < 8 >-1.2 * 10 < 9 > CFU / mL; performing staged fermentation on the sheepskin and the bacterial suspension according to a ratio of 1: 3 at 30-35 DEG C for 18 hours, and supplementing a 0.1% glucose solution of less than or equal to 5% during the fermentation; washing with distilled water and draining; the marinating liquid contains 8% of table salt, 2% of anise, 1% of cinnamon and 0.5% of clove. The method is used for producing edible sheepskin products with flexible texture and up-to-standard microbiological indexes.

The invention discloses an automatic quality detection system for a food production line, and relates to the technical field of food detection.The system collects food surface images in real time through an industrial camera, and an appearance feature data set is generated after preprocessing; using a deep learning model constructed by a convolutional neural network to extract appearance features, forming a food defect data set, calculating an appearance score through an image processing algorithm, and evaluating the appearance score and a surface flaw score threshold value; component content and microbial pollution data are acquired and processed in real time through near infrared spectrum analysis and multi-sensor detection, and component content scores and pollution scores are generated; performing quality judgment by calculating a comprehensive quality score and evaluating the comprehensive quality score with a set comprehensive quality score threshold value; in an abnormal condition, the system triggers an alarm and informs a worker through wireless communication; the whole system realizes automatic, precise and multi-dimensional detection of food quality, and the production detection efficiency and the food safety control capability are remarkably improved.

The invention discloses a food safety pretreatment and detection device and method, and relates to the technical field of food detection.The device comprises a box body, a control module, a pretreatment module and an ATP biological fluorescence detector, the control module is integrated on the box body, and the pretreatment module is integrated on the box body; the control module comprises a microcomputer control system, a visual operation panel, a nixie tube display and a key regulator; the pretreatment module comprises a concentrator for purging and concentrating a sample, a centrifugal machine for separating the sample, a uniform mixing instrument for uniformly mixing the sample and a water bath kettle for performing constant-temperature incubation on the sample; the ATP biological fluorescence detector is integrated in the box body and is used for carrying out microbial pollution detection on the food contact surface; the control module is suitable for adjusting and controlling operation parameters through the key adjuster when any unit in the concentrator, the centrifugal machine, the blending instrument or the water bath kettle operates, and the operation parameters are displayed on the visual operation panel in real time. The invention further provides a detection method, and on-site, rapid and intelligent food safety detection is achieved.

The invention discloses a microbial safety prediction method and system for retort pouch food and a medium, and relates to the field of retort pouch food sterilization, and the method comprises the following steps: obtaining basic data, microbial pollution data and microbial heat resistance data of to-be-processed retort pouch food; calculating the initial pollution amount of the to-be-processed retort pouch food based on the configuration proportion of the different raw materials and the number of microorganisms corresponding to the different raw materials; based on the initial pollution amount, the formula parameters and the process parameters, the total pollution amount of the to-be-processed retort pouch food is calculated; based on the total pollution amount and the process parameters, calculating the process sterilization amount of the to-be-processed retort pouch food; calculating target sterilization time of the to-be-processed retort pouch food based on the process sterilization amount, the process parameters and the microorganism heat-resistant data; and when the target sterilization time is less than or equal to the preset sterilization time, the to-be-processed retort pouch food does not have the problems of microbial safety and corruption in subsequent production. According to the method, the microbial safety of the retort pouch food is effectively predicted in the earlier stage of production of the retort pouch food.

The invention relates to the technical field of microbiological detection, in particular to a public area microbiological pollution risk assessment system based on spectral analysis, which comprises a spectrum acquisition module used for acquiring a Raman spectrum curve of microorganisms contained in the air of a public area; the spectrum correction module is used for carrying out baseline correction on the Raman spectrum curve, specifically, baseline values of the Raman spectrum curve at all wavelengths are determined, and the corrected Raman spectrum curve is obtained; the feature analysis module is used for identifying the types of the microorganisms, specifically, the feature evaluation value of each wave crest is calculated, all the wave crests are distinguished, and a feature main peak and a feature shoulder peak are obtained; extracting characteristic peaks contained in the acromion; identifying the types of microorganisms in the air; and the risk assessment module assesses the microbial pollution risk of the public area. According to the method, misjudgment and missing detection caused by characteristic peak overlapping are reduced, and the accuracy of microorganism variety identification is improved, so that the pollution risk of microorganisms in a public area is accurately evaluated.

The utility model provides a preparation subpackaging device which comprises a main pipeline, branch pipelines, a driving pump and an exhaust pipe, the near end of the main pipeline is communicated with a stock solution bag, a plurality of branch pipelines are communicated with the main pipeline, one of the branch pipelines is communicated with the far end of the main pipeline, each branch pipeline is independently provided with a subpackaging valve and a subpackaging container, and the driving pump is communicated with the exhaust pipe. The driving pump is arranged on the main pipeline between the branch pipeline and the stock solution bag, the exhaust pipe is arranged on the main pipeline between the driving pump and the stock solution bag, a main valve is arranged on the main pipeline between the exhaust pipe and the stock solution bag, and an exhaust valve is arranged on the exhaust pipe. According to the preparation split charging device, only one exhaust pipe is arranged, contact with outside air is reduced, the risk that microorganisms pollute a pipeline is reduced, the split charging amount is controlled through the weighing sensor, the split charging uniformity and accuracy can be ensured, high-speed split charging is achieved, residual preparations in the pipeline can be emptied to be recycled, and the production efficiency is improved. And waste is reduced.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. The methods may comprise gene-editing at least a portion of the TILs to enhance their therapeutic efficacy. Such TILs find use in therapeutic treatment regimens.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The invention relates to the technical field of food microorganism detection, and discloses a real-time detection method for microbial pollution of normal-temperature pure goat milk. The method comprises the following steps: dividing a target goat milk sample into a plurality of detection units representing independent production batches, collecting microbiological detection data, physicochemical property data and spectral characteristic data of each unit, and integrating to form a multi-source detection data set; and constructing a pollution risk scoring index and a quality stability evaluation index based on the data set, and dynamically generating an initial pollution risk value through an early warning trigger mechanism and model operation. And dynamically correcting the initial pollution risk value according to the time sequence correlation of the adjacent detection units to generate a corrected risk value. And performing sorting processing on the corrected risk values, creating a risk level priority form, and connecting a real-time monitoring platform to perform visual display and alarm output. According to the method, multi-source data fusion analysis is realized, high-risk batches can be identified in time, and an efficient means is provided for quality control of normal-temperature pure goat milk.

The invention provides a reverse osmosis optimal dosing system and method based on ultraviolet sterilization, and the method comprises the following steps: firstly, the system monitors the influent microorganism load and water quality in real time, dynamically calculates the required ultraviolet dosage, and precisely adjusts the power of an ultraviolet sterilizer; secondly, according to the residual microorganism risk and the water inlet flow after ultraviolet treatment, the feeding amount of an oxidizing bactericide (such as sodium hypochlorite) is accurately adjusted according to a PID control strategy combining feedforward and feedback; meanwhile, according to the oxidation-reduction potential of the sterilized water body, a reducing agent (such as sodium hydrogen sulfite) is intelligently added to protect the reverse osmosis membrane. And finally, efficient cooperation of ultraviolet physical sterilization and chemical sterilization is achieved, on the premise that strict control over microbial pollution is guaranteed, the consumption and operation cost of chemical agents are remarkably reduced, generation of by-products is reduced, the service life of a reverse osmosis membrane is prolonged, and the intelligent level of system operation and the overall economic benefits are improved.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The invention discloses a flowing-center wiredrawing cheese snack with a multi-layer composite structure and a preparation method of the flowing-center wiredrawing cheese snack, and belongs to the technical field of food processing. The snack is formed by sequentially compounding an outer cheese layer, a middle wiredrawing glutinous rice skin layer and an inner flowing core cheese layer from outside to inside, wherein the water activity (Aw) of the three-layer structure is accurately regulated and controlled to be in a gradient relation (0.65-0.70-0.75-0.80-0.85) which is sequentially increased from outside to inside. Through cooperation of the gradient structure, a specific formula and a key process, moisture migration and texture deterioration of the product in the storage process are effectively inhibited. According to the invention, the long-term stable storage (more than or equal to 8.5 months) of the product under the condition of 25 + / -2 DEG C is successfully realized, and multiple common technical bottlenecks of flow center solidification, wire drawing performance degradation, out-of-control moisture migration, microbial pollution and the like of the existing flow center wire drawing product in a normal-temperature environment are systematically solved.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The invention relates to the technical field of dough fermentation, and discloses a high-quality dough fermenter with a rapid circulating heating function, which comprises a fermentation box, a groove is arranged at the bottom of the fermentation box, a vertical pipe is communicated with the bottom of the groove, an anti-blocking box is communicated with the bottom of the vertical pipe, a drain pipe is communicated with the bottom of the anti-blocking box, and a filter screen is arranged on the inner side of the anti-blocking box. A material receiving box is arranged on one side of the anti-blocking box in a penetrating mode, the material receiving box is in sliding connection with the fermentation box, the material receiving box abuts against the filter screen, and the device further comprises an anti-blocking unit arranged in the anti-blocking box. According to the high-quality dough fermenter with the rapid circulating heating function, condensate water is drained in time through a drainage structure, the problems that the humidity in the fermenter is too high, dough adheres and collapses are solved, and meanwhile the risk of microbial contamination is reduced. And the anti-blocking unit continuously cleans impurities on the filter screen, maintains smooth drainage and ensures stable fermentation environment, so that the quality of baked finished products is improved, the maintenance and replacement cost is reduced, and the service life of equipment is prolonged.

The invention discloses an aquatic product cold chain transportation monitoring system which is characterized in that the system comprises a hardware layer and a software layer, the hardware layer comprises a sensing control module, a structure bearing module and a communication interaction module, and the software layer comprises a data management module, an intelligent calculation module and a business application module; the sensing control module comprises an environment sensing sub-module and an equipment control sub-module, the structure bearing module comprises a compartment main body sub-module and a cargo protection sub-module, and the communication interaction module comprises an edge calculation sub-module and a vehicle-mounted interaction sub-module. According to the application, a real-time monitoring-dynamic prediction-intelligent regulation and control microbial inhibition system is constructed, a high-precision sensor is used for collecting environmental data in real time, and an LSTM-Attention model is combined for accurately pre-judging the growth trend of microorganisms, so that the effects of early warning before the colony count reaches a dangerous threshold value and automatically coordinating equipment to start targeted bacteriostatic measures are achieved, and the safety of the system is improved. And the loss rate of aquatic products caused by microbial pollution is reduced.

The invention discloses a rare-earth-based bionic nano material as well as a preparation method and application thereof, and relates to the field of microbial pollution and anti-corrosion treatment. According to the invention, the active center of lactonase can be simulated by using a zinc-based material, and ester bonds in N-acyl homoserine lactone (AHL) molecules can be hydrolyzed by using the lactonase so as to block bacterial quorum sensing (QS) among bacterial florae, so that the antibacterial effect is achieved. Meanwhile, the outer layer of zinc is provided with a cerium element as a shell, so that on one hand, permeation of oxygen, moisture and corrosive ions can be prevented; on the other hand, the structure has hydrophobicity, and corrosion of moisture and salt ions to a metal matrix is further reduced through the hydrophobicity. Cerium and an oxide thereof have certain antibacterial ability and can have a synergistic effect with zinc, Ce can promote generation of Zn-N and generate strong electron interaction, better hydrophobicity is shown, and attachment and growth of microorganisms on the metal surface can be further inhibited.

The invention relates to the technical field of biology, and particularly discloses a sample treatment method for oat karyotype detection, which comprises the following steps: S1, root tip pretreatment: taking root tip tissues after germination of oat seeds, and treating the root tip tissues in an N2O gas environment containing 0.05-0.1% colchicine for 2-3 hours, then transferring into a glacial acetic acid-ethanol mixed solution which is precooled to 4 DEG C and fixing for 15-20 minutes; s2, performing enzymolysis treatment: cutting the fixed root tip tissue to be 1-2mm, soaking the cut root tip tissue into enzymatic hydrolysate, and performing enzymolysis at 35-38 DEG C for 30-40 minutes; according to the method, the treatment time and pressure of the root tip tissue in the N2O gas environment containing 0.05%-0.1% of colchicine are controlled to be 0.5-0.8 MPa, and the treatment temperature is controlled to be 20-25 DEG C. According to the method, stagnation in the middle stage of cell division is effectively promoted, so that the dispersion effect of chromosomes is remarkably improved; the improved stationary liquid not only can effectively fix the chromosome morphology and prevent the chromosome morphology from changing in the subsequent treatment process, but also can significantly inhibit microbial contamination and prolong the preservation time of the sample.

The invention discloses a drinking water targeted sterilization and disinfection device based on an asymmetric vacancy catalytic material and application of the drinking water targeted sterilization and disinfection device to removal of pathogenic microorganism polluted water, and relates to the technical field of water treatment. The device comprises a raw water tank, a peristaltic pump, a filter column, an aeration pump, an aeration diffuser and a clean water tank, the filter column comprises a column body, an asymmetric vacancy catalytic material, a bearing plate, a gravel filler and an oyster shell filler, a bearing plate is arranged at the bottom of the column body, and a gravel filler, an oyster shell filler and an asymmetric vacancy catalytic material are sequentially arranged on the bearing plate from bottom to top. According to the invention, an asymmetric vacancy catalytic material is precisely constructed, an electron polarization effect is induced, and a filter column drinking water treatment device is combined, so that a novel RO / 1O2 synergistic oxidation disinfection technology which is efficient, targeted and low in byproduct generation is constructed. Safety guarantee is provided for rural distributed water supply, the safety and reliability of rural drinking water can be comprehensively improved, and sustainable development of rural drinking water safety engineering is promoted.

A composition (100) for inhibiting growth of microbial contamination is disclosed The composition includes a plurality of volatile antimicrobial components (110) including at least one of potassium sorbate and sorbic acid. The composition further includes a plurality of non-volatile antimicrobial components (120) including natamycin. A weight ratio of the plurality of volatile antimicrobial components to the plurality of non-volatile antimicrobial components is in the range of 10:2 to 10:1. The composition is placed at a distance from a target surface to inhibit microbial growth on the target surface by releasing the plurality of volatile antimicrobial components and the plurality of non-volatile antimicrobial components into a surrounding air. The target surface is exposed to microbial contaminants. moisture and humidity.

This invention discloses a method for preparing a water-based protective coating for pharmaceutical aluminum foil, relating to the field of pharmaceutical packaging materials technology. By using water as the core solvent, it solves the environmental pollution problems caused by traditional solvent-based coatings, while eliminating the fire safety hazards of flammable and explosive organic solvents. Furthermore, the addition of hydroxylated chitosan improves the adhesion between the coating and the aluminum foil substrate, effectively preventing coating peeling during transportation and storage, and also endows the coating with excellent antibacterial properties, reducing the risk of microbial contamination of pharmaceuticals. The introduction of polycaprolactone diol-modified acrylate significantly improves the coating's flexibility, preventing cracking at low temperatures, while also taking into account solvent resistance and abrasion resistance. This solves the problem of poor balance between flexibility and solvent resistance in existing water-based coatings, thereby ensuring the integrity of pharmaceutical packaging and the long-term stability of pharmaceutical quality.

The application discloses a stable anti-reversibility microbial community structure and a construction method and application thereof, and relates to the technical field of microbial community construction, and in particular to a stable anti-reversibility microbial community structure and a construction method and application thereof.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

An antimicrobial aqueous liquid composition consisting of: (i) equal to or greater than 0.1% w / w of an antimicrobial fraction; (ii) equal to or less than 99.9% w / w water; (iii) 0 to 5% w / w diluents, excipients or carriers other than water, wherein the sum of the percentage amounts of (i), (ii) and (ii) is 100%, and wherein the total weight of the antimicrobial fraction in the composition consists of: (a) 60 to 70% w / w lactic acid or a water-soluble salt thereof; (b) 4 to 10% w / w citric acid or a water-soluble salt thereof; (c) 5 to 15% w / w anionic polycarboxylate surfactant; (d) 9 to 15% w / w α-Olefin (C12-C18) sulfonate; (e) 6 to 10% w / w anionic maleic acid co-polymer surfactant. Also claimed is a method of combating contamination of a site with a microorganism. The amounts of a-e present in the composition may be, respectively, 1.35-1.75 wt.%, 0.025 to 0.325 percent by weight, 0.05 to 0.5 % by weight 0.125 to 0.325 with the balance being water.

The invention relates to the technical field of medical drugs, and particularly discloses a honeysuckle flower and mango capsule and a preparation method thereof. The honeysuckle flower and mango capsule comprises a medicine-containing core material and a plant capsule shell, the medicine-containing core material comprises the following raw materials: a medicine extraction composition, a filling agent and an adhesive. The medicine extraction composition is prepared by adopting a specific extraction method, effective components in all plants can be rapidly extracted through reflux extraction and decoction extraction, damage to the effective components is reduced, and the extraction efficiency is high; and effective components in the raw materials are extracted more thoroughly through a percolation method, so that the purity and quality of the extract are improved. The medicine-containing core material further comprises a filling agent and an adhesive besides the medicine extraction composition, the stability of effective components in the medicine extraction composition can be improved, continuous and stable release of the medicine effective components is guaranteed, and the treatment effect of the honeysuckle flower and mango capsule is improved. The plant capsule shell is selected as a hard capsule and is not easily polluted by microorganisms, so that the stability of the medicine is improved, and the medicine is convenient for patients to take.

An in-situ and in-flight microbial sterilization system for eliminating microbial contamination. The system comprises a housing having at least two openings and a passageway, the passageway configured to receive an object, and at least one pneumatic dust removal system, at least one heater and at least one non-ionizing radiation emitting device, the at least one pneumatic dust removal system. The heater and non-ionizing radiation emitting device are configured to sterilize an object within the passageway.

The invention discloses preparation of an antibacterial surfactant and application of the antibacterial surfactant in a pesticide suspending agent, and belongs to the technical field of pesticide preparations.The method comprises the steps that esterified mPEG 500 succinic acid monoester, pentaerythritol and 12-hydroxystearic acid are subjected to condensation to obtain mPEG / hydroxystearic acid co-modified star-shaped pentaerythritol; the product and glycidyl trimethyl ammonium chloride are subjected to a ring-opening reaction under the alkaline condition, a four-function modified star polymer is obtained, and then the four-function modified star polymer is compounded with modified silicon dioxide to prepare the antibacterial surfactant. Through a double dispersion mechanism of a star-shaped structure and modified silicon dioxide, the problems that a traditional surfactant is easily polluted by microorganisms, particles are agglomerated due to hard water, and the suspension dispersion is poor are solved, the antibacterial property, the hard water resistance, the wetting property and the penetrating property are improved, the surfactant has long-acting stable dispersion and synergistic antibacterial ability, and the service life of the surfactant is prolonged. The storage and transportation of the pesticide suspending agent and the use requirements of farmlands with different water qualities are met.

The utility model discloses a freeze-drying medicine bottle washing device, which relates to the field of bottle washing device structures, and comprises a supporting box body, a bottle washing device, a bottle washing device, a bottle washing device and a bottle washing device, the moving plate is arranged to be capable of entering the supporting box body from the inlet after being driven; the supporting rod piece is arranged in the supporting box body and used for supporting the movable plate piece; and the cleaning liquid pipeline system is arranged above the supporting rod piece and comprises a purified water supply system and an injection water supply system, and the movable plate piece is further arranged to transport the medicine bottles to be cleaned from the purified water supply system to the injection water supply system. Through the arrangement of the purified water supply system and the injection water supply system, medicine bottles can be sequentially cleaned with purified water and injection water in the supporting box body under the cooperation of the movable plate, so that the purposes of improving the cleaning efficiency and preventing the medicine bottles from being polluted by microorganisms again are achieved.

The utility model provides a kind of sterilization respiratory bag, belong to aseptic pharmaceutical technical field, including first piece and second piece, the bottom end and both sides of first piece and second piece are respectively heat-seal connection to form the bag body with the opening of upper end;First piece includes third piece and fourth piece sequentially heat-seal connection from top to bottom, and the middle heat-seal line is formed between third piece and fourth piece, second piece and third piece are transparent sheet layer, and fourth piece is breathable antibacterial sheet layer;The upper portion of bag body is provided with easy tear opening, and easy tear opening is located between opening and middle heat-seal line.The utility model provides a kind of sterilization respiratory bag, and easy tear opening is arranged between middle heat-seal line and opening, so that tear open operation is located in transparent sheet layer, avoid the risk that fiber debris or plastic microparticle is produced by breathable antibacterial sheet layer tearing, effectively avoid that microorganism pollution aseptic environment is carried by microparticle, and provide safeguard for the operation safety of medical, pharmaceutical and other high cleanliness scene.