189 results about "Microbial contamination" patented technology

The invention discloses a system and method for identifying microbial contamination in food and cosmetics based on AI identification, and the method comprises the steps: collecting to-be-detected food or cosmetics as a detection sample, and obtaining the bacterial colony growth state of microorganisms in the detection sample; the method comprises the following steps: acquiring a microbial growth image through microscopic imaging equipment, and preprocessing the microbial growth image to obtain a target area of a microbial colony; constructing an AI recognition model by adopting a convolutional neural network, and obtaining the species and quantity of microorganisms in the detection sample; parameters of the AI recognition model are adjusted in real time according to the types and the number of the microorganisms, a preset microorganism limited standard threshold value is combined, a visual analysis report is generated, the AI recognition model is combined with a Faster R-CNN target detection method, precise classification and number statistics of the types of the microorganisms are achieved, a complete microorganism pollution analysis report is formed, and the method is suitable for popularization and application. And the accuracy and scientificity of the final identification result are improved.

The invention relates to a processing method of fermented edible sheepskin based on plant lactobacillus, and belongs to the technical field of food processing. Aiming at the problems of fiber damage, high microbial pollution risk, texture hardening after marinating and the like caused by chemical treatment in the traditional sheepskin processing, the invention provides a solution of combining biological fermentation with gradient temperature control. The method is characterized by comprising the following steps: soaking sheepskin (subjected to unhairing treatment) in a 4-6% sodium carbonate solution (40 DEG C, 2 hours) for pretreatment; activating the plant lactobacillus until the viable bacterium concentration is 8 * 8 * 10 < 8 >-1.2 * 10 < 9 > CFU / mL; performing staged fermentation on the sheepskin and the bacterial suspension according to a ratio of 1: 3 at 30-35 DEG C for 18 hours, and supplementing a 0.1% glucose solution of less than or equal to 5% during the fermentation; washing with distilled water and draining; the marinating liquid contains 8% of table salt, 2% of anise, 1% of cinnamon and 0.5% of clove. The method is used for producing edible sheepskin products with flexible texture and up-to-standard microbiological indexes.

The invention discloses an automatic quality detection system for a food production line, and relates to the technical field of food detection.The system collects food surface images in real time through an industrial camera, and an appearance feature data set is generated after preprocessing; using a deep learning model constructed by a convolutional neural network to extract appearance features, forming a food defect data set, calculating an appearance score through an image processing algorithm, and evaluating the appearance score and a surface flaw score threshold value; component content and microbial pollution data are acquired and processed in real time through near infrared spectrum analysis and multi-sensor detection, and component content scores and pollution scores are generated; performing quality judgment by calculating a comprehensive quality score and evaluating the comprehensive quality score with a set comprehensive quality score threshold value; in an abnormal condition, the system triggers an alarm and informs a worker through wireless communication; the whole system realizes automatic, precise and multi-dimensional detection of food quality, and the production detection efficiency and the food safety control capability are remarkably improved.

The invention discloses an antibacterial film loaded with a fucoxanthin nano material and a preparation method of the antibacterial film. The antibacterial film is prepared by taking a nitrogen-rich hollow carbon material-fucoxanthin (NHC-FX) nano material as an antibacterial photosensitizer, chitosan as a substrate of a film and glycerol as a plasticizer of the film through a solution casting method. The antibacterial film disclosed by the invention is good in hydrophobicity and high in stability, can reduce transmission of ultraviolet light, and has a good photodynamic antibacterial effect. The antibacterial food packaging material can be used as an antibacterial food packaging material or a preservative film, can prevent food from being polluted by microorganisms, and can isolate damage of ultraviolet rays to the food.

The invention discloses a food safety pretreatment and detection device and method, and relates to the technical field of food detection.The device comprises a box body, a control module, a pretreatment module and an ATP biological fluorescence detector, the control module is integrated on the box body, and the pretreatment module is integrated on the box body; the control module comprises a microcomputer control system, a visual operation panel, a nixie tube display and a key regulator; the pretreatment module comprises a concentrator for purging and concentrating a sample, a centrifugal machine for separating the sample, a uniform mixing instrument for uniformly mixing the sample and a water bath kettle for performing constant-temperature incubation on the sample; the ATP biological fluorescence detector is integrated in the box body and is used for carrying out microbial pollution detection on the food contact surface; the control module is suitable for adjusting and controlling operation parameters through the key adjuster when any unit in the concentrator, the centrifugal machine, the blending instrument or the water bath kettle operates, and the operation parameters are displayed on the visual operation panel in real time. The invention further provides a detection method, and on-site, rapid and intelligent food safety detection is achieved.

The invention discloses a microbial safety prediction method and system for retort pouch food and a medium, and relates to the field of retort pouch food sterilization, and the method comprises the following steps: obtaining basic data, microbial pollution data and microbial heat resistance data of to-be-processed retort pouch food; calculating the initial pollution amount of the to-be-processed retort pouch food based on the configuration proportion of the different raw materials and the number of microorganisms corresponding to the different raw materials; based on the initial pollution amount, the formula parameters and the process parameters, the total pollution amount of the to-be-processed retort pouch food is calculated; based on the total pollution amount and the process parameters, calculating the process sterilization amount of the to-be-processed retort pouch food; calculating target sterilization time of the to-be-processed retort pouch food based on the process sterilization amount, the process parameters and the microorganism heat-resistant data; and when the target sterilization time is less than or equal to the preset sterilization time, the to-be-processed retort pouch food does not have the problems of microbial safety and corruption in subsequent production. According to the method, the microbial safety of the retort pouch food is effectively predicted in the earlier stage of production of the retort pouch food.

The invention relates to the technical field of microbiological detection, in particular to a public area microbiological pollution risk assessment system based on spectral analysis, which comprises a spectrum acquisition module used for acquiring a Raman spectrum curve of microorganisms contained in the air of a public area; the spectrum correction module is used for carrying out baseline correction on the Raman spectrum curve, specifically, baseline values of the Raman spectrum curve at all wavelengths are determined, and the corrected Raman spectrum curve is obtained; the feature analysis module is used for identifying the types of the microorganisms, specifically, the feature evaluation value of each wave crest is calculated, all the wave crests are distinguished, and a feature main peak and a feature shoulder peak are obtained; extracting characteristic peaks contained in the acromion; identifying the types of microorganisms in the air; and the risk assessment module assesses the microbial pollution risk of the public area. According to the method, misjudgment and missing detection caused by characteristic peak overlapping are reduced, and the accuracy of microorganism variety identification is improved, so that the pollution risk of microorganisms in a public area is accurately evaluated.

The utility model provides a preparation subpackaging device which comprises a main pipeline, branch pipelines, a driving pump and an exhaust pipe, the near end of the main pipeline is communicated with a stock solution bag, a plurality of branch pipelines are communicated with the main pipeline, one of the branch pipelines is communicated with the far end of the main pipeline, each branch pipeline is independently provided with a subpackaging valve and a subpackaging container, and the driving pump is communicated with the exhaust pipe. The driving pump is arranged on the main pipeline between the branch pipeline and the stock solution bag, the exhaust pipe is arranged on the main pipeline between the driving pump and the stock solution bag, a main valve is arranged on the main pipeline between the exhaust pipe and the stock solution bag, and an exhaust valve is arranged on the exhaust pipe. According to the preparation split charging device, only one exhaust pipe is arranged, contact with outside air is reduced, the risk that microorganisms pollute a pipeline is reduced, the split charging amount is controlled through the weighing sensor, the split charging uniformity and accuracy can be ensured, high-speed split charging is achieved, residual preparations in the pipeline can be emptied to be recycled, and the production efficiency is improved. And waste is reduced.

The intestinal flora collector comprises a collection mechanism used for collecting flora, the collection mechanism comprises a mirror tube, a sampling hole is formed in the lower end of the mirror tube, a plurality of LED lamps and a camera are installed at the edge of the outer side of the sampling hole, the lower end of the mirror tube is rotationally connected with an electric drive cover, and the electric drive cover is connected with a power source. A sampling hole is formed in the upper end of the base, a sampling column is slidably connected into the sampling hole, two matched arc-shaped collectors are rotatably connected to the lower end of the sampling column, a cavity is formed in the sampling column, two rotating shafts are rotatably connected into the cavity and are in transmission connection through a first gear set, and unfolding wheels are coaxially and fixedly connected to the outsides of the two rotating shafts; an unfolding line is wound outside the unfolding wheel, and the end, away from the unfolding wheel, of the unfolding line penetrates through the collecting column in a sliding mode and is fixedly connected with the two arc-shaped collectors. According to the invention, the bacterial flora is not polluted by other microorganisms during collection, and the biological activity and stability of the bacterial flora are maintained during preservation of the bacterial flora.

The utility model relates to the field of microbial fermentation, and discloses a microbial strain fermentation device convenient for material supplementation, which comprises a fermentation tank, the fermentation tank is provided with a fermentation inner cavity, and the top end and the bottom end of the fermentation tank are respectively and fixedly connected with a feed pipe and a discharge pipe which are communicated with the fermentation inner cavity. A plurality of material supplementing pipes communicated with the fermentation inner cavity are fixedly mounted at the top end of the fermentation tank along the outer side of the feeding pipe, two semicircular connecting arc blocks are fixedly mounted at the top ends of the material supplementing pipes, and the two ends of the two connecting arc blocks are fixedly connected with a plurality of bolts through a plurality of mounting blocks respectively; the top end of the fermentation tank is sleeved with a rubber sleeve located on the inner sides of the two connecting arc blocks, the top ends of the two closed connecting arc blocks are rotationally connected with a protection assembly, and the possibility of being polluted by external microorganisms can be reduced to a great extent while material supplementation is facilitated.

This clothes care apparatus comprises: a steam generating device; a water tank configured to supply water to the steam generating device and collect the water discharged from the steam generating device; a microorganism detection sensor configured to collect first sensor data related to the amount of microorganisms attached to the water tank; a water level sensor configured to collect second sensor data related to the water level of the water tank; a sterilizing device configured to sterilize water stored in the water tank; and a control unit that controls the sterilizing device on the basis of a first function related to the second sensor data when a microorganism contamination level determined on the basis of the first sensor data is less than a reference contamination level, and controls the sterilizing device on the basis of a second function related to the second sensor data when the microorganism contamination level is equal to or greater than the reference contamination level.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. The methods may comprise gene-editing at least a portion of the TILs to enhance their therapeutic efficacy. Such TILs find use in therapeutic treatment regimens.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The invention relates to the technical field of food packaging, in particular to an antibacterial fresh-keeping packaging film for food packaging and a preparation method of the antibacterial fresh-keeping packaging film, and aims at solving the problems that a traditional food packaging film is insufficient in antibacterial performance and not ideal in fresh-keeping effect. According to the preparation method, polyethylene resin and polylactic resin are taken as main raw materials, so that the packaging film is endowed with excellent mechanical property and barrier property, the oxidation process of food can be slowed down, the damage of oxygen to the food is reduced, the loss of nutritional ingredients in the food is prevented, and the freshness and nutritional ingredients of the food can be effectively kept; the modified nano-silver particles are added into the food, so that the growth and metabolic activities of microorganisms such as various bacteria and molds can be effectively inhibited, the risk that the food is polluted by the microorganisms is remarkably reduced, the deterioration of the food due to microbial decomposition is avoided, and the food quality is improved. Therefore, the quality and taste of the food are better maintained, and the shelf life of the food is prolonged.

The invention relates to the technical field of food microorganism detection, and discloses a real-time detection method for microbial pollution of normal-temperature pure goat milk. The method comprises the following steps: dividing a target goat milk sample into a plurality of detection units representing independent production batches, collecting microbiological detection data, physicochemical property data and spectral characteristic data of each unit, and integrating to form a multi-source detection data set; and constructing a pollution risk scoring index and a quality stability evaluation index based on the data set, and dynamically generating an initial pollution risk value through an early warning trigger mechanism and model operation. And dynamically correcting the initial pollution risk value according to the time sequence correlation of the adjacent detection units to generate a corrected risk value. And performing sorting processing on the corrected risk values, creating a risk level priority form, and connecting a real-time monitoring platform to perform visual display and alarm output. According to the method, multi-source data fusion analysis is realized, high-risk batches can be identified in time, and an efficient means is provided for quality control of normal-temperature pure goat milk.

The present invention relates to an antimicrobial composition for plant culture medium that exhibits excellent antimicrobial effects due to the high physicochemical stability thereof, allowing the active ingredients to remain intact and unaffected during high-temperature and high-pressure sterilization processes. Moreover, the composition can be conveniently added to plant culture media, enhancing user convenience. Therefore, the composition can be effectively used to prevent microbial contamination in plant tissue culture media or hydroponic nutrient solutions.

The invention provides a reverse osmosis optimal dosing system and method based on ultraviolet sterilization, and the method comprises the following steps: firstly, the system monitors the influent microorganism load and water quality in real time, dynamically calculates the required ultraviolet dosage, and precisely adjusts the power of an ultraviolet sterilizer; secondly, according to the residual microorganism risk and the water inlet flow after ultraviolet treatment, the feeding amount of an oxidizing bactericide (such as sodium hypochlorite) is accurately adjusted according to a PID control strategy combining feedforward and feedback; meanwhile, according to the oxidation-reduction potential of the sterilized water body, a reducing agent (such as sodium hydrogen sulfite) is intelligently added to protect the reverse osmosis membrane. And finally, efficient cooperation of ultraviolet physical sterilization and chemical sterilization is achieved, on the premise that strict control over microbial pollution is guaranteed, the consumption and operation cost of chemical agents are remarkably reduced, generation of by-products is reduced, the service life of a reverse osmosis membrane is prolonged, and the intelligent level of system operation and the overall economic benefits are improved.

The embodiment of the invention provides a container feeding device, and relates to the technical field of feeding equipment, the container feeding device is used for solving the technical problem that the interior of a container is easily polluted by microorganisms, and the container feeding device comprises a conveying assembly used for conveying the container on a nest plate to a feeding position; the two driving assemblies are respectively a first driving assembly and a second driving assembly; the two executive components are a first executive component and a second executive component respectively; a placing hole for placing a container is formed in the nest plate; the first driving assembly drives the first execution piece to move, and the first execution piece takes out the container at the feeding position from the placement hole; the second driving assembly drives the second execution piece to move, the second execution piece takes down the container on the first execution piece, and the second execution piece places the container on the bearing piece at the material receiving position; in the process that the first execution piece and the second execution piece move the container at the feeding position to the bearing piece at the receiving position, the first execution piece and the second execution piece are not located above the top end of the container.

The utility model discloses sterile feeding and discharging equipment with a cross valve group. The sterile feeding and discharging equipment comprises the cross valve group, a steam pressure reduction unit and a steam barrier unit, the cross valve group comprises a T-shaped sterile valve, an L-shaped sterile valve and a four-way joint; the steam pressure reducing unit comprises a steam source, a steam filter, a steam pressure reducing valve, a steam pressure gauge, a steam stop valve and a thick pipe; the steam barrier unit comprises a first automatic angle seat valve, a second automatic angle seat valve and a steam trap. According to the utility model, the flow direction of the product is switched by utilizing the cross valve group, the steam pressure reduction unit provides clean and stable-pressure steam for the steam barrier of the cross valve group, and the steam barrier unit provides sterile guarantee for the production of the cross valve group, so that steam sterilization can be carried out under the condition of high temperature for a long time; the sterilization procedure before production of the sterile tank is met, pollution of microorganisms is avoided, the selection of feeding and discharging at the same time is provided for production of the sterile tank, the sterile condition in the production process is guaranteed, pollution of the microorganisms is avoided, and the requirement for long-shelf-life products is met.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The invention discloses a flowing-center wiredrawing cheese snack with a multi-layer composite structure and a preparation method of the flowing-center wiredrawing cheese snack, and belongs to the technical field of food processing. The snack is formed by sequentially compounding an outer cheese layer, a middle wiredrawing glutinous rice skin layer and an inner flowing core cheese layer from outside to inside, wherein the water activity (Aw) of the three-layer structure is accurately regulated and controlled to be in a gradient relation (0.65-0.70-0.75-0.80-0.85) which is sequentially increased from outside to inside. Through cooperation of the gradient structure, a specific formula and a key process, moisture migration and texture deterioration of the product in the storage process are effectively inhibited. According to the invention, the long-term stable storage (more than or equal to 8.5 months) of the product under the condition of 25 + / -2 DEG C is successfully realized, and multiple common technical bottlenecks of flow center solidification, wire drawing performance degradation, out-of-control moisture migration, microbial pollution and the like of the existing flow center wire drawing product in a normal-temperature environment are systematically solved.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The invention relates to the technical field of dough fermentation, and discloses a high-quality dough fermenter with a rapid circulating heating function, which comprises a fermentation box, a groove is arranged at the bottom of the fermentation box, a vertical pipe is communicated with the bottom of the groove, an anti-blocking box is communicated with the bottom of the vertical pipe, a drain pipe is communicated with the bottom of the anti-blocking box, and a filter screen is arranged on the inner side of the anti-blocking box. A material receiving box is arranged on one side of the anti-blocking box in a penetrating mode, the material receiving box is in sliding connection with the fermentation box, the material receiving box abuts against the filter screen, and the device further comprises an anti-blocking unit arranged in the anti-blocking box. According to the high-quality dough fermenter with the rapid circulating heating function, condensate water is drained in time through a drainage structure, the problems that the humidity in the fermenter is too high, dough adheres and collapses are solved, and meanwhile the risk of microbial contamination is reduced. And the anti-blocking unit continuously cleans impurities on the filter screen, maintains smooth drainage and ensures stable fermentation environment, so that the quality of baked finished products is improved, the maintenance and replacement cost is reduced, and the service life of equipment is prolonged.

The invention discloses an aquatic product cold chain transportation monitoring system which is characterized in that the system comprises a hardware layer and a software layer, the hardware layer comprises a sensing control module, a structure bearing module and a communication interaction module, and the software layer comprises a data management module, an intelligent calculation module and a business application module; the sensing control module comprises an environment sensing sub-module and an equipment control sub-module, the structure bearing module comprises a compartment main body sub-module and a cargo protection sub-module, and the communication interaction module comprises an edge calculation sub-module and a vehicle-mounted interaction sub-module. According to the application, a real-time monitoring-dynamic prediction-intelligent regulation and control microbial inhibition system is constructed, a high-precision sensor is used for collecting environmental data in real time, and an LSTM-Attention model is combined for accurately pre-judging the growth trend of microorganisms, so that the effects of early warning before the colony count reaches a dangerous threshold value and automatically coordinating equipment to start targeted bacteriostatic measures are achieved, and the safety of the system is improved. And the loss rate of aquatic products caused by microbial pollution is reduced.

The invention discloses a rare-earth-based bionic nano material as well as a preparation method and application thereof, and relates to the field of microbial pollution and anti-corrosion treatment. According to the invention, the active center of lactonase can be simulated by using a zinc-based material, and ester bonds in N-acyl homoserine lactone (AHL) molecules can be hydrolyzed by using the lactonase so as to block bacterial quorum sensing (QS) among bacterial florae, so that the antibacterial effect is achieved. Meanwhile, the outer layer of zinc is provided with a cerium element as a shell, so that on one hand, permeation of oxygen, moisture and corrosive ions can be prevented; on the other hand, the structure has hydrophobicity, and corrosion of moisture and salt ions to a metal matrix is further reduced through the hydrophobicity. Cerium and an oxide thereof have certain antibacterial ability and can have a synergistic effect with zinc, Ce can promote generation of Zn-N and generate strong electron interaction, better hydrophobicity is shown, and attachment and growth of microorganisms on the metal surface can be further inhibited.

The invention relates to the technical field of biology, and particularly discloses a sample treatment method for oat karyotype detection, which comprises the following steps: S1, root tip pretreatment: taking root tip tissues after germination of oat seeds, and treating the root tip tissues in an N2O gas environment containing 0.05-0.1% colchicine for 2-3 hours, then transferring into a glacial acetic acid-ethanol mixed solution which is precooled to 4 DEG C and fixing for 15-20 minutes; s2, performing enzymolysis treatment: cutting the fixed root tip tissue to be 1-2mm, soaking the cut root tip tissue into enzymatic hydrolysate, and performing enzymolysis at 35-38 DEG C for 30-40 minutes; according to the method, the treatment time and pressure of the root tip tissue in the N2O gas environment containing 0.05%-0.1% of colchicine are controlled to be 0.5-0.8 MPa, and the treatment temperature is controlled to be 20-25 DEG C. According to the method, stagnation in the middle stage of cell division is effectively promoted, so that the dispersion effect of chromosomes is remarkably improved; the improved stationary liquid not only can effectively fix the chromosome morphology and prevent the chromosome morphology from changing in the subsequent treatment process, but also can significantly inhibit microbial contamination and prolong the preservation time of the sample.

The invention discloses a drinking water targeted sterilization and disinfection device based on an asymmetric vacancy catalytic material and application of the drinking water targeted sterilization and disinfection device to removal of pathogenic microorganism polluted water, and relates to the technical field of water treatment. The device comprises a raw water tank, a peristaltic pump, a filter column, an aeration pump, an aeration diffuser and a clean water tank, the filter column comprises a column body, an asymmetric vacancy catalytic material, a bearing plate, a gravel filler and an oyster shell filler, a bearing plate is arranged at the bottom of the column body, and a gravel filler, an oyster shell filler and an asymmetric vacancy catalytic material are sequentially arranged on the bearing plate from bottom to top. According to the invention, an asymmetric vacancy catalytic material is precisely constructed, an electron polarization effect is induced, and a filter column drinking water treatment device is combined, so that a novel RO / 1O2 synergistic oxidation disinfection technology which is efficient, targeted and low in byproduct generation is constructed. Safety guarantee is provided for rural distributed water supply, the safety and reliability of rural drinking water can be comprehensively improved, and sustainable development of rural drinking water safety engineering is promoted.

The invention discloses a trithiothiotriazine trisodium salt corrosion inhibitor and its preparation method and application, and belongs to the technical field of corrosion inhibitor synthesis. The invention uses thiocyanuric acid as a raw material, and successfully grafts a carbon-carbon double bond to it by reacting with bromopropylene to generate an intermediate 2,4-dimercapto-6-mercaptopropenyl-1,3,5-triazine monomer, and then polymerizes it with allylthiourea, adds an initiator under nitrogen protection, and heats, stirs, purifies, and dries in a water bath to obtain a corrosion inhibitor; the corrosion inhibitor has excellent corrosion inhibition and bactericidal properties at the same time, can effectively solve the corrosion and microbial contamination problems in oil and gas field pipelines, still has excellent stability under high temperature environment, and meets the use requirements under extreme working conditions such as oil and gas fields.