107 results about "Microbial contamination" patented technology

The invention discloses a food safety pretreatment and detection device and method, and relates to the technical field of food detection.The device comprises a box body, a control module, a pretreatment module and an ATP biological fluorescence detector, the control module is integrated on the box body, and the pretreatment module is integrated on the box body; the control module comprises a microcomputer control system, a visual operation panel, a nixie tube display and a key regulator; the pretreatment module comprises a concentrator for purging and concentrating a sample, a centrifugal machine for separating the sample, a uniform mixing instrument for uniformly mixing the sample and a water bath kettle for performing constant-temperature incubation on the sample; the ATP biological fluorescence detector is integrated in the box body and is used for carrying out microbial pollution detection on the food contact surface; the control module is suitable for adjusting and controlling operation parameters through the key adjuster when any unit in the concentrator, the centrifugal machine, the blending instrument or the water bath kettle operates, and the operation parameters are displayed on the visual operation panel in real time. The invention further provides a detection method, and on-site, rapid and intelligent food safety detection is achieved.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. The methods may comprise gene-editing at least a portion of the TILs to enhance their therapeutic efficacy. Such TILs find use in therapeutic treatment regimens.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The invention relates to the technical field of food microorganism detection, and discloses a real-time detection method for microbial pollution of normal-temperature pure goat milk. The method comprises the following steps: dividing a target goat milk sample into a plurality of detection units representing independent production batches, collecting microbiological detection data, physicochemical property data and spectral characteristic data of each unit, and integrating to form a multi-source detection data set; and constructing a pollution risk scoring index and a quality stability evaluation index based on the data set, and dynamically generating an initial pollution risk value through an early warning trigger mechanism and model operation. And dynamically correcting the initial pollution risk value according to the time sequence correlation of the adjacent detection units to generate a corrected risk value. And performing sorting processing on the corrected risk values, creating a risk level priority form, and connecting a real-time monitoring platform to perform visual display and alarm output. According to the method, multi-source data fusion analysis is realized, high-risk batches can be identified in time, and an efficient means is provided for quality control of normal-temperature pure goat milk.

The invention provides a reverse osmosis optimal dosing system and method based on ultraviolet sterilization, and the method comprises the following steps: firstly, the system monitors the influent microorganism load and water quality in real time, dynamically calculates the required ultraviolet dosage, and precisely adjusts the power of an ultraviolet sterilizer; secondly, according to the residual microorganism risk and the water inlet flow after ultraviolet treatment, the feeding amount of an oxidizing bactericide (such as sodium hypochlorite) is accurately adjusted according to a PID control strategy combining feedforward and feedback; meanwhile, according to the oxidation-reduction potential of the sterilized water body, a reducing agent (such as sodium hydrogen sulfite) is intelligently added to protect the reverse osmosis membrane. And finally, efficient cooperation of ultraviolet physical sterilization and chemical sterilization is achieved, on the premise that strict control over microbial pollution is guaranteed, the consumption and operation cost of chemical agents are remarkably reduced, generation of by-products is reduced, the service life of a reverse osmosis membrane is prolonged, and the intelligent level of system operation and the overall economic benefits are improved.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The invention discloses a flowing-center wiredrawing cheese snack with a multi-layer composite structure and a preparation method of the flowing-center wiredrawing cheese snack, and belongs to the technical field of food processing. The snack is formed by sequentially compounding an outer cheese layer, a middle wiredrawing glutinous rice skin layer and an inner flowing core cheese layer from outside to inside, wherein the water activity (Aw) of the three-layer structure is accurately regulated and controlled to be in a gradient relation (0.65-0.70-0.75-0.80-0.85) which is sequentially increased from outside to inside. Through cooperation of the gradient structure, a specific formula and a key process, moisture migration and texture deterioration of the product in the storage process are effectively inhibited. According to the invention, the long-term stable storage (more than or equal to 8.5 months) of the product under the condition of 25 + / -2 DEG C is successfully realized, and multiple common technical bottlenecks of flow center solidification, wire drawing performance degradation, out-of-control moisture migration, microbial pollution and the like of the existing flow center wire drawing product in a normal-temperature environment are systematically solved.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

The invention discloses an aquatic product cold chain transportation monitoring system which is characterized in that the system comprises a hardware layer and a software layer, the hardware layer comprises a sensing control module, a structure bearing module and a communication interaction module, and the software layer comprises a data management module, an intelligent calculation module and a business application module; the sensing control module comprises an environment sensing sub-module and an equipment control sub-module, the structure bearing module comprises a compartment main body sub-module and a cargo protection sub-module, and the communication interaction module comprises an edge calculation sub-module and a vehicle-mounted interaction sub-module. According to the application, a real-time monitoring-dynamic prediction-intelligent regulation and control microbial inhibition system is constructed, a high-precision sensor is used for collecting environmental data in real time, and an LSTM-Attention model is combined for accurately pre-judging the growth trend of microorganisms, so that the effects of early warning before the colony count reaches a dangerous threshold value and automatically coordinating equipment to start targeted bacteriostatic measures are achieved, and the safety of the system is improved. And the loss rate of aquatic products caused by microbial pollution is reduced.

The invention discloses a drinking water targeted sterilization and disinfection device based on an asymmetric vacancy catalytic material and application of the drinking water targeted sterilization and disinfection device to removal of pathogenic microorganism polluted water, and relates to the technical field of water treatment. The device comprises a raw water tank, a peristaltic pump, a filter column, an aeration pump, an aeration diffuser and a clean water tank, the filter column comprises a column body, an asymmetric vacancy catalytic material, a bearing plate, a gravel filler and an oyster shell filler, a bearing plate is arranged at the bottom of the column body, and a gravel filler, an oyster shell filler and an asymmetric vacancy catalytic material are sequentially arranged on the bearing plate from bottom to top. According to the invention, an asymmetric vacancy catalytic material is precisely constructed, an electron polarization effect is induced, and a filter column drinking water treatment device is combined, so that a novel RO / 1O2 synergistic oxidation disinfection technology which is efficient, targeted and low in byproduct generation is constructed. Safety guarantee is provided for rural distributed water supply, the safety and reliability of rural drinking water can be comprehensively improved, and sustainable development of rural drinking water safety engineering is promoted.

A composition (100) for inhibiting growth of microbial contamination is disclosed The composition includes a plurality of volatile antimicrobial components (110) including at least one of potassium sorbate and sorbic acid. The composition further includes a plurality of non-volatile antimicrobial components (120) including natamycin. A weight ratio of the plurality of volatile antimicrobial components to the plurality of non-volatile antimicrobial components is in the range of 10:2 to 10:1. The composition is placed at a distance from a target surface to inhibit microbial growth on the target surface by releasing the plurality of volatile antimicrobial components and the plurality of non-volatile antimicrobial components into a surrounding air. The target surface is exposed to microbial contaminants. moisture and humidity.

This invention discloses a method for preparing a water-based protective coating for pharmaceutical aluminum foil, relating to the field of pharmaceutical packaging materials technology. By using water as the core solvent, it solves the environmental pollution problems caused by traditional solvent-based coatings, while eliminating the fire safety hazards of flammable and explosive organic solvents. Furthermore, the addition of hydroxylated chitosan improves the adhesion between the coating and the aluminum foil substrate, effectively preventing coating peeling during transportation and storage, and also endows the coating with excellent antibacterial properties, reducing the risk of microbial contamination of pharmaceuticals. The introduction of polycaprolactone diol-modified acrylate significantly improves the coating's flexibility, preventing cracking at low temperatures, while also taking into account solvent resistance and abrasion resistance. This solves the problem of poor balance between flexibility and solvent resistance in existing water-based coatings, thereby ensuring the integrity of pharmaceutical packaging and the long-term stability of pharmaceutical quality.

The application discloses a stable anti-reversibility microbial community structure and a construction method and application thereof, and relates to the technical field of microbial community construction, and in particular to a stable anti-reversibility microbial community structure and a construction method and application thereof.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

An in-situ and in-flight microbial sterilization system for eliminating microbial contamination. The system comprises a housing having at least two openings and a passageway, the passageway configured to receive an object, and at least one pneumatic dust removal system, at least one heater and at least one non-ionizing radiation emitting device, the at least one pneumatic dust removal system. The heater and non-ionizing radiation emitting device are configured to sterilize an object within the passageway.

The invention discloses preparation of an antibacterial surfactant and application of the antibacterial surfactant in a pesticide suspending agent, and belongs to the technical field of pesticide preparations.The method comprises the steps that esterified mPEG 500 succinic acid monoester, pentaerythritol and 12-hydroxystearic acid are subjected to condensation to obtain mPEG / hydroxystearic acid co-modified star-shaped pentaerythritol; the product and glycidyl trimethyl ammonium chloride are subjected to a ring-opening reaction under the alkaline condition, a four-function modified star polymer is obtained, and then the four-function modified star polymer is compounded with modified silicon dioxide to prepare the antibacterial surfactant. Through a double dispersion mechanism of a star-shaped structure and modified silicon dioxide, the problems that a traditional surfactant is easily polluted by microorganisms, particles are agglomerated due to hard water, and the suspension dispersion is poor are solved, the antibacterial property, the hard water resistance, the wetting property and the penetrating property are improved, the surfactant has long-acting stable dispersion and synergistic antibacterial ability, and the service life of the surfactant is prolonged. The storage and transportation of the pesticide suspending agent and the use requirements of farmlands with different water qualities are met.

The utility model discloses a freeze-drying medicine bottle washing device, which relates to the field of bottle washing device structures, and comprises a supporting box body, a bottle washing device, a bottle washing device, a bottle washing device and a bottle washing device, the moving plate is arranged to be capable of entering the supporting box body from the inlet after being driven; the supporting rod piece is arranged in the supporting box body and used for supporting the movable plate piece; and the cleaning liquid pipeline system is arranged above the supporting rod piece and comprises a purified water supply system and an injection water supply system, and the movable plate piece is further arranged to transport the medicine bottles to be cleaned from the purified water supply system to the injection water supply system. Through the arrangement of the purified water supply system and the injection water supply system, medicine bottles can be sequentially cleaned with purified water and injection water in the supporting box body under the cooperation of the movable plate, so that the purposes of improving the cleaning efficiency and preventing the medicine bottles from being polluted by microorganisms again are achieved.

The utility model provides a kind of sterilization respiratory bag, belong to aseptic pharmaceutical technical field, including first piece and second piece, the bottom end and both sides of first piece and second piece are respectively heat-seal connection to form the bag body with the opening of upper end;First piece includes third piece and fourth piece sequentially heat-seal connection from top to bottom, and the middle heat-seal line is formed between third piece and fourth piece, second piece and third piece are transparent sheet layer, and fourth piece is breathable antibacterial sheet layer;The upper portion of bag body is provided with easy tear opening, and easy tear opening is located between opening and middle heat-seal line.The utility model provides a kind of sterilization respiratory bag, and easy tear opening is arranged between middle heat-seal line and opening, so that tear open operation is located in transparent sheet layer, avoid the risk that fiber debris or plastic microparticle is produced by breathable antibacterial sheet layer tearing, effectively avoid that microorganism pollution aseptic environment is carried by microparticle, and provide safeguard for the operation safety of medical, pharmaceutical and other high cleanliness scene.

The invention discloses an efficient offline cleaning method for reverse osmosis membrane microbial contamination, and belongs to the technical field of reverse osmosis membrane cleaning. The method comprises the following steps: firstly, carrying out cleaning pretreatment on a reverse osmosis membrane, then putting the reverse osmosis membrane into a cleaning water tank with nutrients for carbon source circulation, carrying out gas production fermentation in an anaerobic environment, strengthening biological membrane loosening in an aerobic-anaerobic environment, and finally, carrying out offline high-pressure cleaning, alkali washing, acid washing and flushing. The standard water yield recovery rate gt of the reverse osmosis membrane after cleaning is carried out by the method; the pressure difference increasing rate after cleaning is gt; 1.8 Pa / m < 2 > / h; the standard water yield recovery rate gt of the cleaned reverse osmosis membrane is determined; and after cleaning, the differential pressure increase rate is reduced to 1.4 Pa / m < 2 > / h or below. The method is more friendly to membrane cleaning and has wide applicability.

The invention relates to the technical field of image recognition, and discloses a food surface microbial contamination detection method and device and a medium, and the method comprises the steps: dividing a surface image through the surface attribute of a target food; collecting an original composite light signal of the sampling area, and carrying out achromatic differential processing on the original composite light signal to obtain a microbial fluorescence signal; generating dual-channel light intensity time sequence data according to the microbial fluorescence signal and the timestamp, and calculating the dynamic pollution light intensity ratio of the surface of the target food according to the dual-channel light intensity time sequence data; triggering a secondary detection instruction on the surface of the target food according to the dynamic pollution light intensity ratio, and performing time gating sampling on the microbial fluorescence signal according to the secondary detection instruction to obtain a microbial attenuation coefficient; analyzing fluorescence lifetime fingerprints of microorganisms on the surface of the target food through the microorganism attenuation coefficient, and determining the pollution degree of the microorganisms on the surface of the target food according to the fluorescence lifetime fingerprints. According to the invention, the accuracy of food surface microbial contamination detection can be improved.

The invention provides a method for rapidly detecting benzoic acid and sorbic acid in smoked and cooked sausages and hams, and belongs to the technical field of food detection and analysis. The smoked and cooked sausage and ham product is traditional processed meat with extremely regional characteristics, is rich in various nutrient substrates, is easily polluted by microorganisms to cause spoilage, and is short in shelf life. Among many preservatives, benzoic acid and sorbic acid have the most wide application range, and for the purpose of prolonging the shelf life of food, many enterprises often have the situations of over-range addition and over-limit addition of the two preservatives. According to the national food safety standard GB 5009.28-2016, the technical defects of long detection period, tedious degreasing treatment, low recovery rate, sample matrix interference and the like exist when liquid chromatography is used for detecting large-batch samples. The method is innovated on the basis of a liquid chromatography, based on the characteristic that the fat content of the smoked and cooked sausage and ham is relatively low, a sample is not degreased in a pretreatment link, and the purified sample liquid is diluted by 5 times and then is detected on a machine; benzoic acid and sorbic acid in the smoked and cooked sausage and ham are measured by accurately adjusting the pH value of a mobile phase, improving a chromatographic column and optimizing instrument parameters. The method is simple and practicable, is safe to operate, reduces the cost, and meets the daily detection requirements.

The invention relates to a preparation method of a sodium alginate-gelatin composite film containing cedrela sinensis extract, in particular to the technical field of food packaging materials.The preparation method comprises the following steps that S1, gelatin with the weight / volume being 4% is dissolved in distilled water, glycerin with the weight / mass being 30% is added to serve as a plasticizer, stirring is conducted till the gelatin is completely dissolved, and a gelatin solution is obtained; according to the invention, the cedrela sinensis extract is used as a natural plant extract, is rich in bioactive components such as polyphenols and flavones, and can effectively scavenge free radicals and slow down oxidation reaction of food, such that the composite film has excellent performance in the aspect of prolonging the shelf life of the food, can effectively prevent the food from going bad and keep the freshness and nutritional value of the food, and at the same time, the composite film has a good application prospect. The cedrela sinensis extract has a good inhibition effect on common food pathogenic bacteria such as escherichia coli and staphylococcus aureus, and can provide additional antibacterial protection in the packaging process, reduce microbial pollution and prolong the shelf life of food.

The utility model belongs to the technical field of traditional Chinese medicine granule production equipment, particularly relates to a Xiaoqinglong granule boiling, granulating and drying integrated device, and aims to solve the problems that when materials are put into an existing device, a putting opening and the materials are completely exposed to the outside, and the materials exposed in an open environment are polluted by external dust and microorganisms. In order to solve the problems in the prior art that the product quality safety is seriously influenced, the utility model provides the following scheme: the Xiaoqinglong granule boiling, granulating and drying integrated device comprises a base, and a granulating and drying tank body is mounted on the surface of the base; the utility model has the beneficial effects that: a totally-closed feeding and adhesive conveying system is adopted, material and solution pollution is avoided, the reject ratio of products is reduced, the granulation cleanness and the product quality are ensured, the special structure of the material distribution plate is matched with annular hot air radiation to form double-channel hot air diversion, the materials are uniformly heated, the moisture content fluctuation is reduced, and the production efficiency is improved. And the quality uniformity of products is effectively improved, and reliable guarantee is provided for production of Xiaoqinglong granules.

This invention relates to the field of beer production technology and discloses a mixing device and process for beer production. The device includes a tank with an upper water pipe and a lower liquid pipe at its upper and lower ends, respectively. The side wall of the tank has inlet and outlet ports. An internal filter screen is installed inside the tank. The tank also contains a stirring shaft, a rotating ring, a vertical rod, an anchor-shaped paddle, and a tillering paddle. The stirring shaft is rotatably mounted inside the tank. This mixing device, through the arrangement of the stirring shaft, rotating ring, vertical rod, anchor-shaped paddle, and tillering paddle, and in conjunction with a lifting control mechanism, achieves synchronous reverse lifting and lowering movements of the anchor-shaped paddle and the tillering paddle. This eliminates the need to transfer the mash and malt between the mashing tank and the filter tank, thus completing the integrated mashing and malt washing process. This effectively shortens the production process, reduces equipment footprint, avoids heat loss and material residue during wort transfer, improves raw material utilization and production efficiency, reduces the risk of microbial contamination, and ultimately ensures the quality of the produced beer.

The present invention provides improved and / or shortened methods for expanding TILs and producing therapeutic populations of TILs, including novel methods for expanding TIL populations in a closed system that lead to improved efficacy, improved phenotype, and increased metabolic health of the TILs in a shorter time period, while allowing for reduced microbial contamination as well as decreased costs. Such TILs find use in therapeutic treatment regimens.

This utility model relates to the field of cell culture technology, specifically to a resealable, moisture-proof, and oxidation-proof dry powder cell culture medium bottle. It includes a storage bottle with a lower screw cap screwed onto its spout. An upper screw cap is screwed onto the top of the lower screw cap. A fixing plate is located in the center of the inner cavity of the lower screw cap. A rotating rod is rotatably connected to the top of the fixing plate via a bearing. A rotating plate is fixedly sleeved onto the outside of the rotating rod. Multiple storage cavities are formed on the rotating plate, and a baffle is located in the center of the inner cavity of each storage cavity. This utility model solves the problem that traditional dry powder cell culture medium bottles are typically single-use sealed structures. Although desiccants are added during packaging, they only provide some protection during the initial packaging. Once used and opened multiple times, their protective effect is greatly reduced, providing favorable conditions for microbial growth. Microbial contamination can damage the components of the culture medium and affect the normal progress of cell culture.

The invention relates to the technical field of cell separation, and particularly discloses a cell separation device which comprises a main body, the rotating platform is arranged in the main body, and the rotating platform rotates at a high speed to generate centrifugal force, so that target cells are separated from other components; the number of the bases is four, and the four bases are distributed in an annular array mode relative to the center axis of the rotating platform; the first reset spring provides upward elastic force for the sliding block, the second reset spring provides downward elastic force for the clamping seat, and when the centrifugal tube moves along with the clamping seat, the first reset spring simultaneously absorbs power of the clamping seat, protects the centrifugal tube, gently and uniformly mixes, prevents cells from settling to block a filter membrane, but avoids generation of foam; four centrifugal tubes are uniformly mixed in the rotating platform at the same time, so that the experiment process is reduced, a sample is uniformly mixed in a sterile environment, the possibility that the sample is polluted by microorganisms outside the sterile environment is reduced, and the accuracy and the treatment effect of a subsequent experiment are influenced.

The invention discloses a preparation method of a low-salt shallow-fermented sausage, and belongs to the technical field of sausage preparation, and the method comprises the steps of raw material pretreatment, material mixing, sausage filling, fermentation and the like. Then hot air fermentation and cold air fermentation are sequentially performed to promote the growth and reproduction of beneficial microorganisms and improve the microbial diversity of the sausages, so that accumulation and conversion of flavor substances are promoted, the healthy sausages which are rich in fragrance and low in salt are obtained, the fermentation time is greatly shortened, and the fermentation efficiency is improved; the preservative is prepared at the same time to spray the fermented sausages, and the preservative can slowly and continuously release natural antibacterial components to inhibit microbial contamination, so that the safety of the sausages is improved, the storage life of the sausages is prolonged, and the preservative has a good application prospect.

Provided are a preparation method for an antibacterial gel film based on fish scale gelatin compounded with probiotics, and the use thereof. Fish scale gelatin is compounded with probiotics to prepare a gel film with excellent properties. Meanwhile, prolamin, trehalose and an amino acid are supplemented to facilitate the formation of a unique spatial structure in the fish scale gelatin, with uniform pores and a compact structure, thereby further enhancing the physicochemical properties thereof. The prepared gel film exhibits a good antibacterial effect and can be used for the storage and preservation of perishable foods. Therefore, the present invention not only achieves the utilization of fish scale waste, but also solves the problems of spoilage and deterioration caused by the microbial contamination of the prepared product, thereby achieving a significant technical effect.