437 results about "Milk sample" patented technology

The present invention includes compositions, kits and methods useful for the detection of mastitis in an animal. These agents and methods are primarily directed to a method of detecting the presence of mastitis, including sub-clinical mastitis, in cows, involving incubating a sample of milk from the cow with an agent that binds to lactoferrin such as, e.g., a monoclonal antibody specific for lactoferrin, and then detecting bound lactoferrin. The invention includes lateral-flow immunoassay methods and devices for assessing the presence of lactoferrin in milk samples.

The invention relates to a quantitative detection method for thermal-denaturation and non-denaturation bovine alpha-lactalbumin in milk and milk products by applying an enzymolysis-liquid chromatography and mass spectrometry combination technology. The quantitative detection method comprises the steps as follows: taking a certain amount of milk or milk samples, dissolving and diluting the milk or milk samples in water to obtain solution with total protein content being about 1mg/mL; after volume metering, correctly sucking 500 mu L, adding an internal standard substance, reacting disulfide bond with dithiothreitol (DTT), alkylating to protect sulfydryl produced in reaction by iodoacetamide (IAA), and then conducting constant-temperature and constant-time enzymolysis with trypsin; and separating enzymolysis products by reversed phase liquid chromatography, conducting detection with a mass spectrum multiple reaction monitoring (MRM) manner, and calculating the result by an internal standard method. The quantitation limit of the method is 0.001g/100g; when adding amount is 0.2, 1.7 and 5.0g/100g, the recovery rate is 98.9-110.8% (n is equal to 6) and repeatability: RSD (Relative Standard Deviation) is smaller than 7.6%; and the quantitative detection method can be applicable to the quantitative detection of samples with different contents of bovine alpha-lactalbumin.

Method and apparatus for testing a milk for mastitis wherein the milk sample is admixed with a detergent under condition that increase the viscosity of the milk sample; the increased viscosity mixture is subjected to a further procedure for further increasing its viscosity; and the further increased viscosity mixture is then tested for an attribute of somatic cells, such as DNA. This determination is then used to establish if the cow from whom the milk came has mastitis.

The invention discloses a method for distinguishing milk doped with ewe's milk by a near infrared spectrum, and the method comprises the following steps: 1) selecting a milk sample; 2) collecting the near infrared spectrum; and 3) carrying out cluster analysis and establishing a qualitative distinguishing model, thus carrying out the cluster analysis on raw milk and adulteration milk and realizing qualitative judgment. By using the method, the milk doped with ewe's milk can be detected rapidly qualitatively and quantitatively; and when the weight percentage of the ewe's milk in the adulteration milk is greater than or equal to 5 percent, the accuracy of detection can reach 100 percent, and the detection time of a single sample is within 1 minute.

The invention provides a quick near-infrared measuring device and method for milk ingredients and relates to the technical field of near-infrared spectroscopic analysis. The device comprises a constant current driving unit, an LED (Light Emitting Diode) component unit, a sample pool unit, a photoelectric detection unit, a signal processing and control unit, a man-machine interface unit, and the like. A diffuse transmission measuring mode is adopted, sample absorbance values of sixteen short-wave near-infrared absorbing wavelengths are utilized and a prediction model in the device is used, thereby simultaneously measuring the indexes in the milk, such as fat, protein, milk sugar, non-fat milk solid, total milk solid, water, and the like. The quick near-infrared measuring device has the advantages that sixteen short-wave near-infrared absorbing wavelengths are used for quickly measuring the main ingredients of milk, thereby being small in data volume, high in measuring speed and suitable for on-site application. The device is small in volume, low in power consumption, light in weight and convenient in carrying, thereby being capable of meeting the requirement for quickly on-site analyzing the main ingredients of a milk sample.

The invention belongs to the field of milk product analysis and particularly relates to a spectral fingerprint identification method for milk, mare milk, camel milk, goat milk and buffalo milk. The invention relates to the intermediate infrared spectroscopy. The method comprises the following steps: 1) taking fresh milk, mare milk, camel milk, goat milk and buffalo milk samples; 2) scanning the samples in a mid-red spectral range to obtain mid-infrared spectral data; 3) preprocessing original mid-infrared spectrum to remove abnormal values; 4) dividing the preprocessed data set into a trainingset and a test set according to a stratified sampling principle; 5) performing dimension reduction processing on the training set to improve the model training speed; 6) establishing a model for distinguishing milk, mare milk, camel milk, goat milk and buffalo milk through 10-fold cross validation by using algorithms such as nearest neighbors on the training set, and evaluating and screening themodel by using the use accuracy, the balance precision and the Kappa coefficient; and 7) estimating the generalization performance of the optimal model.

This invention relates to a device and a method for sampling of milk. The device includes a collecting member (15, 15′) arranged to receive milk samples from a milk line (1), which is arranged to transport milk from one animal (3) at a time, and a passage (8a, 12″, 13) arranged to allow a milk flow from the milk line (1) to the collecting member (15, 15′). The device comprises flow means (11, 19) arranged to provide a milk flow, from the animal (3), through at least a part of the passage (8a, 12″, 13) at least a time period before a milk sample is to be taken in order to rinse at least the part of the passage (8a, 12″, 13) from milk residues from a previously milked animal.

A method for detecting milk components comprises a model building step and a milk detecting step. The model building step is used for building and storing a near infrared spectrum classification and correction model and utilizing the ultrasonic self-exciting frequency to classify the near infrared spectrum of different types of milk and build a near infrared spectrum classification and correctionmodel corresponding to the different types of milk; and the milk detecting step obtains the component content of the milk sample corresponding to the near infrared spectrum classification and correction model according to the ultrasonic self-exciting frequency and near infrared spectrum information of the milk sample to be detected. The device and the method use the ultrasonic self-exciting frequency measuring technology to evaluate the background interference of the milk components, automatically perform the classification of the near infrared spectrum model by means of the ultrasonic and near infrared information fusion and realize quick, accurate, automatic and high-efficient detection of the routine components of the milk.

This invention provides a direct microscopic examination method milk somatic cells/bacteria automatic counter. The automatic counter comprises: a sampling/smearing apparatus, a dyeing apparatus, a washing/drying apparatus, a microscopic detecting apparatus, a convey apparatus for conveying the milk sample as well as glass slide to and out of the sampling/smearing zone, and another convey apparatus for conveying the smeared glass slide to the dyeing zone, washing/drying zone and microscopic detecting zone, which comprises multi-stage glass slide convey mechanisms and glass slide running mechanisms. The automatic analyzer comprises a smearing apparatus, a dyeing apparatus, a washing/drying apparatus a microscopic detecting apparatus and a control apparatus for convey apparatuses. This invention divides direct microscopic examination method milk somatic cells/bacteria analysis and assay into multiple mechanical actions performed by different apparatuses, and sets corresponding working positions and working zones. The working positions and working zones are connected via the convey apparatuses, and can reasonably and effectively perform automatic detection under the control of the control apparatus. The automatic counter has high counting precision and repeat precision, and the working efficiency is largely increased.

The invention relates to a method for detecting melamine in milk which is built based on the inner filter effect (IFE) between cysteine (L-Cys)-modified CdS quantum dots and citric acid ligand coating gold nanoparticles (AuNPs), and belongs to the field of analytical chemistry. The method comprises the detecting steps of preparing the AuNPs, synthesizing and purifying the L-Cys-modified CdS quantum dots, pre-treating a milk sample and detecting the melamine in the milk. According to the principle of recovering the fluorescence intensity of the CdS quantum dots by adopting the AuNPs to cause the fluorescence quenching of the CdS quantum dots and adopting the melamine to induce the aggregation of the AuNPs, the method can simply, quickly and sensitively detect the melamine in the milk so as to provide convenience to future dairy industry.

The content of casein in milk is determined by two measurements of infrared absorbance in a milk sample by infrared spectrometry before and after a separation of the casein. The casein content is calculated by use of absorbance data recorded during the two absorbance measurements. The new method is considerable faster than the known wet-chemical methods, such as the normal wet chemical reference method for casein determination in milk using a Kjeldahl nitrogen determination of the milk sample, then a coagulation of the milk, and finally a Kjeldahl nitrogen determination of the filtrate. Further the new method provides a more reliable accuracy than the know determination using a single infrared analysis of a milk sample.

The invention provides a method for rapidly testing nitrite in fresh milk. The method comprises the following steps: adding 3-6ml of milk sample in a colorimetric tube, then adding 1-2ml of 20% tartaric acid solution and 1-2ml of 0.5% sulfanilic acid solution in turn, shaking evenly, adding 1-2ml of 0.3% N(1-naphty1)-ethylenediamine dihydrochloride solution, wherein the lower dosage of 20% tartaric acid solution is 1ml, the lower dosage of 0.5% sulfanilic acid solution is 1ml, and the lower dosage of 0.3% N(1-naphty1)-ethylenediamine dihydrochloride solution is 1ml; and observing after fully shaking, wherein if different shades of pink appears in the colorimetric tube, the sample is proved to contain nitrite. The size of the selected colorimetric tube with a stopper is 15-20ml. The lower limit detection value of the method is 0.1mu g/ml; and the effects of the method are that the dosage is small, the preservation time is long and the detection sensitivity is high. The reagent kit prepared by the method is more convenient to use.

The present invention refers to a milk sampling apparatus and method for use with an automated milking system. The apparatus comprises a cassette (7) wherein milk sample collecting elements (9) are placed, and at least one filling member (27) capable of being placed above a selected one of said milk sample collecting elements (9) by means of a positioning system, and capable of bringing a milk sample, representatively taken from milk yielded during the milking of an animal by means of said automated milking system, into said selected one of said milk sample collecting elements (9). The positioning system comprises an arm (34) extending in a first dimension (Y) in a plane (XY) above said milk sample collecting elements (9) and being movable in a second direction (X) in said plane (XY), said first (Y) and second (X) directions being orthogonal; a runner (32) holding said filling member (27) and being movable along said arm (34); and first (29) and a second (31) drive means, wherein said first drive means (29) is effective to move said arm (34) and said second drive means (31) is effective to move said runner (32) such that said filling member (27) is positioned above said selected one of said milk sample collecting elements (9).

A milk metering apparatus having a metering chamber 7 which is repeatedly filled and emptied many times in the course of milking an animal is provided with a probe 15 which extends down into the metering chamber 7 and which includes a sampling tube as well as conductivity and light absorption sensors. Milk samples can be taken and conductivity and light absorption measurements can be made during each filling and emptying cycle so that data from the sample analysis and the measurements over the whole period of milking can be compared with corresponding data from previous milkings of the same animal thereby providing valuable information for herd management purposes.

The present invention refers to a milk sampling apparatus for use with an automated milking system, said apparatus comprising a cassette (7) wherein milk sample collecting elements (9) are placed, and at least one filling member (27) capable of being placed above a selected one of said milk sample collecting elements (9) by means of a positioning system, and capable of bringing a milk sample, representatively taken from milk yielded during the milking of an animal by means of said automated milking system, into said selected one of said milk sample collecting elements (9). According to the invention the milk sampling apparatus comprises agitating means, preferably a shaking table (5), capable of agitating said milk sample.

The invention belongs to the technical field of antibacterial drug residue detection in foods, particularly relates to a high-throughput screening method for antibacterial drugs in milk and relates to a method for detecting 120 antibacterial drug residues in milk at the same time. The detection method comprises the steps of (1) building a technology for pretreatment of antibacterial drugs in milk; (2) building a method for detecting antibacterial drugs in milk; (3) building a blank milk spectral library; (4) building a milk antibacterial drug residue spectral library; and (5) comparing the blank milk spectral library with the milk antibacterial drug residue spectral library to obtain the variety and the content of antibacterial drugs in a to-be-detected milk sample. The method for detecting antibacterial drug residues in a milk sample is simple and fast in operation, low in organic reagent dose and high in sensitivity. The variety and the content of antibacterial drugs in a milk sample can be quickly and accurately identified and the method is suitable for monitoring antibacterial drug residues in milk.

The invention provides a purification method for recombination human lactoferrin, animal lactoferrin and lactoperoxidase. The method comprises: taking a milk sample containing recombination human lactoferrin as a raw material, pre-treating to obtain degreased milk, performing cation exchange chromatographic separation on the obtained degreased milk, then under different salt concentration conditions, performing elution separation on lactoferrin and lactoperoxidase, so as to obtain high-purity lactoferrin and lactoperoxidase, and further performing separation on the solution rich in lactoferrin by utilizing hydrophobic interaction chromatography to respectively obtain recombination human lactoferrin and animal source lactoferrin both with the purity of 99% or more. The purity of recombination human lactoferrin obtained by utilizing the purification method provided by the invention is 99% or more, and also recombination human lactoferrin has the physical and chemical activities and physiological functions all similar to those of natural human lactoferrin; and the technology is a one-step purification process, so that the purification method is low in cost, simple and suitable for industrialized large-scale production.

The invention discloses a rapid detection method of the milk protein content based on a dielectric spectrum technique. According to the rapid detection method, dielectric spectra (a relative dielectric constant spectrum and a dielectric loss factor spectrum) of a batch of milk samples within a radio frequency/microwave range are obtained by utilizing a network analyzer (or an impedance analyzer) and a coaxial probe, characteristic dielectric variables which express the milk protein content are extracted from the dielectric spectra, linear or nonlinear prediction models which are used for predicting the milk protein content are established on the basis of the dielectric spectra or the extracted characteristic dielectric variables within the whole frequency range, the models are inspected, and the model of which the root-mean-square error is the minimum is used as the optimum model for predicting the milk protein content. Data of the dielectric spectra under unknown characteristic frequency of the milk samples are substituted into the optimum model, so that the protein content of the samples can be calculated. The rapid detection method has the advantages of rapidity in detection, convenience, high precision, simplicity in operation and capability of real-time online detection.

A method for determining a concentration of at least one component of milk includes measuring impedance values between two electrodes associated with a cell containing a milk sample at each of at least three frequencies, and estimating a concentration of the at least one component from a polynomial expression in which the impedance values at the at least three frequencies are variables. A system for determining a concentration of at least one component of milk includes a sampling cell including a milk sample, electrodes operative to electrify the milk sample with excitation signals at a plurality of frequencies including at least three frequencies, a signal generator for generating the excitation signals, circuitry for determining a relationship between an amplitude and phase of reflected and incident signals obtained from the signal generator, and a processor that receives the relative amplitude and phase and estimates a concentration of at least one component of milk based on a polynomial expression relating measured impedance values at a plurality of frequencies to concentrations of at least one component of milk.

The invention belongs to the technology researching field of cow molecular, in particular to the technical field of preparing the total DNA of the cow. The invention discloses a method for extracting the total DNA from the cow. Because the cow belongs to an economic animal, in order to avoid the stress caused by blood sampling and collecting the tissue of a living cow as well as overcome the problem of containing higher protein pollution of the extracted total DNA of a milk sample, the invention proposes a more convenient source to obtain the DNA, researches out a key technology from purifying the somatocyte of the milk sample to extracting the DNA with high quality, prepares the reagent for purifying the somatocyte of the milk sample and a cracking mixture for extracting the DNA by test, then uses alcohol with different concentration grads to wash, then uses a phenol method to extract and remove the protein and other matters like amylase, finally uses absolute ethyl alcohol to wash and dissolves into TE after being dried. The invention is simple and convenient to sample; the quality of the sample of the total DNA that is extracted is higher; the method is economic, stable and convenient; the invention provides a novel method for the application of the cow molecular.

The invention provides a quantitative detecting method for estrogen in milk. The quantitative detecting method includes steps of 1) performing derivatization pretreatment for a milk sample; and 2) measuring the content of the estrogen in the milk sample by the aid of online solid-phase extraction and two-dimensional liquid chromatogram tandem mass spectrum analysis. Owning to sample derivatization measurement, detection sensitivity is greatly improved. A two-dimensional liquid chromatogram tandem mass spectrum system is used for detecting, accordingly, online automatic purification of the sample is realized, and the detection efficiency of the sample is greatly improved. In addition, the analysis method is fine in recovery rate, high in automation degree and simple and easy in operation, and can fast and synchronously analyze the content of various types of trace estrogen in the sample.

The invention discloses a self-counting microscopic detecting device, which comprises the following parts: microscopic objective lens and digital cam on the draw tube, upper-lower adjusting driving mechanism in the draw tube, driving and transmitting mechanism on the lower end of objective lens and moving out mechanism of glass-carrying piece from transmitting mechanism. The focusing method of microscope comprises the following steps: setting error parameter of brightness gradient value; shooting milk sample image on the glass-carrying piece; adjusting objective lens of microscope; shooting milk sample image on the glass-carrying piece again; comparing brim brightness gradient value of two images; affirming focus; focusing.

The invention discloses a reagent kit for the chemiluminescence enzyme-linked immunoassay of furazolidone, which comprises a kit body, an enzyme label plate arranged in the kit body and a reagent arranged in the kit body, wherein, holes of the enzyme label plate are coated by an envelope antigen which is manufactured through coupling between 3-azyl-2-oxazolone that is a metabolin of furazolidone and ovalbumin; and the reagent contains a polyclonal antibody of furazolidone, an enzyme label secondary antibody that is a goat anti-rabbit antibody marked by horseradish peroxidase, a furazolidone-series standard solution, a concentrated phosphate buffer solution, a concentrated cleaning solution and a luminescent liquid. The reagent kit has the characteristics of high sensitivity, good reproducibility, simplicity, convenience, speediness and accuracy; compared with the traditional color comparison ELISA method, the sensitivity can be improved by one order higher, and the reagent is expected to play an important role in furazolidone residue detection of water used in stock raising, feeds and animal-derived foods (such as milk samples, animal tissue samples and urine samples).