2125 results about "Specific detection" patented technology

This document describes an optical sensor unit and a procedure for the specific detection and identification of biomolecules at high sensitivity in real fluids and tissue homogenates. High detection limits are reached by the combination of i) label-free integrated optical detection of molecular interactions, ii) the use of specific bioconstituents for sensitive detection and iii) planar optical transducer surfaces appropriately engineered for suppression of non-specific binding, internal referencing and calibration. Applications include the detection of prion proteins and identification of those biomolecules which non-covalently interact with surface immobilized prion proteins and are intrinsically involved in the cause of prion related disease.

Methods, compositions and kits which employ one or more ZEB specific detection reagents for detection and localization of ZEB associated molecules in tumor cells are disclosed. Also provided are methods for determining stage and progression of cancer in a mammal based on alterations in ZEB expression levels and subcellular localization. Also provided are methods for treating a cancer in a mammal by modulating ZEB expression levels and activity.

The invention provides a method for genetic analysis in individuals that reveals both the genetic sequences and chromosomal copy number of targeted and specific genomic loci in a single assay. The present invention further provide methods for the sensitive and specific detection of target gene sequences and gene expression profiles.

A system and method for imaging of a sample, e.g., biological sample, are provided. In particular, at least one source electro-magnetic radiation forwarded to the sample and a reference may be generated. A plurality of detectors may be used, at least one of the detectors capable of detecting a signal associated with a combination of at least one first electro-magnetic radiation received from the sample and at least one second electro-magnetic radiation received from the reference. At least one particular detector may have a particular electrical integration time, and can receive at least a portion of the signal for a time duration which has a first portion with a first power level greater than a predetermined threshold and a second portion immediately preceding or following the first portion. The second portion may have a second power level that is less than the predetermined threshold, and extends for a time period which may be, e.g., approximately more than 10% of the particular electrical integration time.

Methods for enhancing the dynamic range for specific detection of one or more analytes in assays using scattered-light detectable particle labels. The methods involve utilizing variations in detection technique and / or signal processing to extend the dynamic range to either or both of lower and higher concentrations.

Sequence-specific nucleic acid hybridization assays are used for the detection of specific genetic sequences as indicators of genetic anomalies, mutations, and disease propensity. In addition, they are used for the detection of various biological agents and infectious pathogens. Because a complementary probe or nucleic acid sequence is required to detect a sequence of interest in a hybridization-based assay, nucleic acid sequencing techniques can rapidly determine the specific probe sequence being used for detection. This allows reverse engineered assays to be produced rapidly. In addition, it enables the circumvention of hybridization-based assays for biological agent or infectious pathogen detection by providing the information necessary to create or alter nucleic acid sequences to produce false positives or false negatives. The present invention provides methods and compositions for inhibiting the identification of specific detection sequences. More specifically, the invention provides masking sequences that mask the identity of specific detection sequences.

The invention provides a method for genetic analysis in individuals that reveals both the genetic sequences and chromosomal copy number of targeted and specific genomic loci in a single assay. The present invention further provides methods for the sensitive and specific detection of target gene sequences and gene expression profiles.

There is disclosed a sensitive method for specific detection of ligands comprising the steps of (a) contacting the sample with reagents capable of forming a reagent complex, which reagent complex comprises a receptor capable of specifically binding to said ligand and a nucleic acid molecule; and (b) detecting any complexes formed by binding of the receptor part of said reagent complex to ligand present in the sample by specifically detecting the presence of the nucleic acid molecule by amplifying a region of the nucleic acid and simultaneously detecting products of the amplification reaction in real-time.

A method for mapping surroundings of a vehicle, objects in the surroundings of the vehicle being detected with the aid of sensors and particular detected objects being described by two coordinate points and also by a position fuzziness assigned to the particular coordinate point, the coordinate points and the position fuzziness values being stored in an interface in the form of data which may be accessed by driver assistance systems of the vehicle. A method is also described for ascertaining the collision probability of a vehicle with an object, in which the surroundings of the vehicle are initially mapped using the method for mapping the surroundings of a vehicle, a travel path to be traveled by the vehicle is ascertained in a subsequent step, and the degree of overlap between the object and the travel path and also the collision probability are finally determined, taking the position fuzziness into account.

Methods for enhancing the dynamic range for specific detection of one or more analytes in assays using scattered-light detectable particle labels. The methods involve utilizing variations in detection technique and / or signal processing to extend the dynamic range to either or both of lower and higher concentrations.

Described herein are novel SCCmec right extremity junction (MREJ) sequences for the detection and / or identification of methicillin-resistant Staphylococcus aureus (MRSA). Disclosed are methods and compositions based on DNA sequences for the specific detection of MREJ sequences designated types xi, xii, xiii, xiv, xv, xvi, xvii, xviii, xix, and xx for diagnostic purposes and / or epidemiological typing.

Provided is an HPV E6, E7 mRNA assay, referenced herein as the “In Cell HPV Assay,” that is capable of sensitive and specific detection of normal cervical cells undergoing malignant transformation as well as abnormal cervical cells with pre-malignant or malignant lesions. The In Cell HPV Assay identifies HPV E6, E7 mRNA via in situ hybridization with oligonucleotides specific for HPV E6, E7 mRNA and quantitates the HPV E6, E7 mRNA via flow cytometry. The In Cell HPV Assay can be carried out in less than three hours directly from liquid-based cervical (“LBC”) cytology specimens. The In Cell HPV Assay provides an efficient and highly sensitive alternative to the Pap smear for determining abnormal cervical cytology.

Embodiments of the present invention relate to devices and methods for detecting cellular products using detection particles having product-specific detection reagents and having a characteristic spectral feature. In particular, devices and methods are provided for measuring secreted cellular products including cytokines. Detection substrates, include microwells having product-specific capture reagents thereon or comprising hydrophobic membranes are described having greater capability to detect products from individual cells in a mixture of heterogeneous cells. With the use of multiple detection particles, multiple cellular products can be detected in a single well. Additionally, using the inherent spectral properties of detection particles, no enzymatic reactions are needed to visualize a secreted product, thereby increasing the sensitivity, reproducibility and ease of use.

The invention provides devices that improve tests for detecting specific cellular, viral, and molecular targets in clinical, industrial, or environmental samples. The invention permits efficient detection of individual microscopic targets at low magnification for highly sensitive testing. The invention does not require washing steps and thus allows sensitive and specific detection while simplifying manual operation and lowering costs and complexity in automated operation. In short, the invention provides devices that can deliver rapid, accurate, and quantitative, easy-to-use, and cost-effective tests.

The present invention provides a rapid and sensitive method for the detection of a West Nile virus (WNV), Japanese encephalitis virus (JEV), St. Louis encephalitis virus (SLEV) and Dengue virus (DENV) and antibodies directed against thereof involving contacting a biological specimen suspected of being infected with WNV, JE, SLE or DEN with a substantially purified and isolated WNV E glycoprotein or subfragment thereof having a native conformation wherein the E glycoprotein or subfragment thereof has a reactivity with antibodies against WNV and a cross-reactivity with antibodies against JEV, SLEV and DENV. The instant invention further provides a rapid, sensitive, and consistent method for the specific detection of WNV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-WNV antibodies but not cross-reactive with antibodies against other flaviviruses such as JEV, SLEV, or DENV. The present invention also provides a rapid, sensitive, and consistent method for the specific detection of DENV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-DENV antibodies but do not cross-react with antibodies against other flaviviruses such as JEV, SLEV, or WNV. Further, the DENV NS5 antigens are serospecific and do not cross react with antibodies to other DENV strains. Thus, the method of the present invention provides a manner by which to discriminate infections by each DENV strain. Further, diagnostic kits for carrying out the methods are provided. The methods and kits for carrying out the methods of the invention are rapid and require as little as 10 minutes to detect a result.

Disclosed is a method for amplifying and detecting polynucleotides which can provide sensitive, specific detection of multiple targets from a clinical specimen within a relatively short time.

An automatic detection method and a system for human body falling-over are based on a wireless network signal transmission technology. The method comprises the following steps: a wireless receiving terminal receives wireless signals from a wireless transmitting terminal and assesses channel state information; abnormity of the channel state information change is identified by using an abnormity detection algorithm; based on a one-class support vector machine which distinguishes a target motion kind from other motion kinds, an abnormity mode that a human body falling-over motion leads to a channel state information change is set as the target motion kind to determine whether a human body falling-over motion happens; if the human body falling-over motion happens, a falling-over alarm signal is emitted. The false alarm rate of the detection method provided by the invention is low; by utilizing a present wireless network and a device, other specific detection devices are not needed, and the detection method is high in universality; and at the same time people to be detected do not need to carry any extra sensing equipment, and inconvenience caused by that people to be detected carry detection equipment is avoided.

Apparatus and methods in which very small volumes of material may be extracted, delivered, interrogated or stimulated via optical, electromagnetic or mechanical means, in vivo or in vitro, for site-specific detection, characterization, stimulation, diagnostics or therapy, comprising optical, fluidic, chemical, electromagnetic and biological techniques applied via a microprobe in a single intra-parenchymal tissue perforation procedure in the brain. The primary use of the device is in neuroscience research, clinical diagnostics and therapeutics applications in the brain, however, the device may also be beneficially applied to other organs and biological systems. Human clinical applications may include neurosurgical intra-operative monitoring, extra-operative chronic monitoring of devices introduced in an operation, and diagnostic monitoring combined with simultaneous neuroimaging.

The present invention provides a novel class of molecules, termed “compomers,” that enable the indirect detection of target molecules, as well as novel target detection reagents and compomer templates that encode compomers. Compomers are linear polymers generated from the compomer template portion of a target detection reagent during the course of an assay. In a given assay, each compomer species is correlated with a different target molecule, e.g., a carbohydrate, lipid, polypeptide, or target nucleic acid, particularly a specific nucleotide sequence within a target nucleic acid molecule. When, for example, a target nucleic acid is present in an assay, a compomer species specifically and uniquely correlated with the particular target (e.g., a known SNP or other genetic variant) is generated directly from a target-specific detection reagent (or indirectly from a larger precursor encoded by the compomer template and from which it is subsequently released), after which it can readily be detected, even in an assay where tens, hundreds, or thousands of different compomer species may be generated, as each compomer species is engineered to differ from the others by a small, resolvable defined characteristic (e.g., a mass increment, a difference in subunit composition, sequence, size, length, etc.). When coupled with highly sensitive detection techniques (e.g., MALDI-TOF mass spectrometry, nucleic acid hybridization, nuclear magnetic resonance, etc.), a large number of different compomer species can be detected in a single reaction, thereby facilitating highly multiplexed analyses of complex samples.

The invention relates to a novel Universal RT-coupled PCR strategy for the specific detection and accurate quantitation of mRNA. Claimed and disclosed are novel Universal reverse transcription (RT) primers, a specific primer mix containing the Universal RT-primers, a transcript specific forward primer and a reverse PCR primer identical to a unique tag sequence, and methods and kits thereof for avoiding the amplification of genomic DNA and / or pseudogenes.

Devices related to a near-drowning behavior detection method fusing UWB indoor location with a video target detection and tracking technology comprise a smart bracelet module, an intelligent monitoring information processing module, a communication module and a display alarm module, the smart bracelet module is used for performing indoor location, the intelligent monitoring information processingmodule is used for processing video data acquired by an intelligent monitoring camera, location information of a smart bracelet and water depth information; and the communication module is used for realizing information transfer among the smart bracelet module, the intelligent monitoring information processing module and the display alarm module. Specific detection steps of the near-drowning behavior detection method are performed according to the following way: performing system initialization; and building a relationship among an image of the intelligent monitoring camera, location of the smart bracelet module and a swimming pool coordinate system, then setting near-drowning conditions, and performing near-drowning determination by virtue of a near-drowning detection algorithm. The near-drowning behavior detection method is simple in main body structure, smart in design concept, good in using effect, high in detection accuracy, low in maintenance cost, friendly in application environment and broad in market prospect.

Method for specific detection of one or more analytes in a sample. The method includes specifically associating any one or more analytes in the sample with a scattered-light detectable particle, illuminating any particle associated with the analytes with light under conditions which produce scattered light from the particle and in which light scattered from one or more particles can be detected by a human eye with less than 500 times magnification and without electronic amplification. The method also includes detecting the light scattered by any such particles under those conditions as a measure of the presence of the analytes.

The present invention provides a rapid and sensitive method for the detection of a West Nile virus (WNV), Japanese encephalitis virus (JEV), St. Louis encephalitis virus (SLEV) and Dengue virus (DENV) and antibodies directed against thereof involving contacting a biological specimen suspected of being infected with WNV, JE, SLE or DEN with a substantially purified and isolated WNV E glycoprotein or subfragment thereof having a native conformation wherein the E glycoprotein or subfragment thereof has a reactivity with antibodies against WNV and a cross-reactivity with antibodies against JEV, SLEV and DENV. The instant invention further provides a rapid, sensitive, and consistent method for the specific detection of WNV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-WNV antibodies but not cross-reactive with antibodies against other flaviviruses such as JEV, SLEV, or DENV. The present invention also provides a rapid, sensitive, and consistent method for the specific detection of DENV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-DENV antibodies but do not cross-react with antibodies against other flaviviruses such as JEV, SLEV, or WNV. Further, the DENV NS5 antigens are serospecific and do not cross react with antibodies to other DENV strains. Thus, the method of the present invention provides a manner by which to discriminate infections by each DENV strain. Further, diagnostic kits for carrying out the methods are provided. The methods and kits for carrying out the methods of the invention are rapid and require as little as 10 minutes to detect a result. The invention also provides monoclonal antibodies against WNV NS5 and DENV NS5 antigen and their use in detecting WNV and DENV infections in a biological sample.

Described herein are novel SCCmec right extremity junction (MREJ) sequences for the detection and / or identification of methicillin-resistant Staphylococcus aureus (MRSA). Disclosed are methods and compositions based on DNA sequences for the specific detection of MREJ sequences designated types xi, xii, xiii, xiv, xv, xvi, xvii, xviii, xix, and xx for diagnostic purposes and / or epidemiological typing.

The invention discloses a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) based PCR (Polymerase Chain Reaction) typing method and the application thereof. The method is a method forperforming DNA detection and typing. The method comprises the following steps: adding a Cas9 protein and sgRNA for targeting target DNA into a conventional PCR reaction system, adding a short-term thermostatic incubation program before the PCR reaction program, and starting a conventional PCR amplification program. The CRISPR based PCR typing method disclosed by the invention adopts a homogeneousdetection technology, and detection can be completed by a PCR amplification step only. By utilizing a specific recognition cutting characteristic of the CRISPR technology on the DNA, the target DNA can be simply, homogeneously, rapidly and sensitively subjected to specific detection and typing, and the method is a novel DNA detection method with high specificity and sensitivity. According to themethod in the invention, human HPV DNA in clinical samples can be successfully detected.

The invention discloses a fluorescent probe for detecting formaldehyde as well as a preparation method and an application of the fluorescent probe. The structure of the fluorescent probe is presented by a formula I (shown in the specification). A kit for detecting formaldehyde comprises a compound represented by the formula I and a solvent. The ''off-on'' type formaldehyde probe for naphthalimide dyes and the specific detection kit have good responses to a formaldehyde solution, can be used for detecting formaldehyde in cells and have the characteristics of being simple and convenient to operate, low in cost, sensitive in response and easy to popularize and apply.