614 results about "Specific detection" patented technology

The invention relates to the technical field of environmental air detection, in particular to an environmental air quality detection device and a detection method thereof.The environmental air quality detection device comprises a base, and a case is fixedly mounted at the top of the base. Through the dual protection mechanism of the air inlet mechanism and the cleaning module, the air quality detection accuracy can be effectively guaranteed, in the specific detection period, induced draft fan blades rotate forwards to form negative pressure to suck air, an exhaust window assists in exhausting air, it is ensured that airflow is stable, and after a detection task is completed, an automatic cleaning program of the air inlet mechanism is started; the cleaning frame and the front cleaning group cooperatively operate to scrape dust on the inner walls of the induced draft fan blades and the air inlet pipe and prevent residual dust from falling off and being mixed into air in subsequent detection, meanwhile, the cleaning module continuously sprays, and under the action of countercurrent air driven by reverse rotation of the induced draft fan blades, cleaning liquid fully covers the air inlet pipe and the induced draft fan blades and dissolves dust and oil stains, so that secondary pollution is avoided; and air entering the case is ensured to be clean, so that real and reliable detection data is ensured.

A diffractive optical neural network device includes a plurality of diffractive substrate layers arranged in an optical path. The substrate layers are formed with physical features across surfaces thereof that collectively define a trained mapping function between an optical input and an optical output. A plurality of groups of optical sensors are configured to sense and detect the optical output, wherein each group of optical sensors has at least one optical sensor configured to capture a positive signal from the optical output and at least one optical sensor configured to capture a negative signal from the optical output. Circuitry and / or computer software receives signals or data from the optical sensors and identifies a group of optical sensors in which a normalized differential signal calculated from the positive and negative optical sensors within each group is the largest or the smallest of among all the groups.

The invention discloses a primer group, a kit and a method for detecting brucella, and belongs to the technical field of gene detection. The primer group comprises two pairs of specific RPA (recombinase polymerase amplification) primers: a primer pair B1F1 (SEQ ID No.3) and a primer pair B1R1 (SEQ ID No.6) targeting a B1 sequence, and a primer pair B2F1 (SEQ ID No.8) and a primer pair B2R1 (SEQ ID No.11) targeting a B2 sequence. The invention also provides a kit containing the primer group, and a detection method based on the RPA-CRISPR-Cas12a. The invention also provides a kit containing the primer group and a detection method based on the RPA-CRISPR-Cas12a. According to the application, the high efficiency of RPA amplification is combined with the ultrahigh specificity of CRISPR-Cas12a detection, the defects of long time consumption, low sensitivity and easy generation of false positive in the traditional method are overcome, rapid, sensitive and specific detection of Brucella is realized, and the application has important application value in early screening and monitoring of Brucella.

The present invention belongs to the field of virus detection technology, and specifically relates to a combination and application of monoclonal antibodies for the specific detection of type 5 adenovirus. The antibody combination includes 8B9 and 4A6, which target different epitopes of the HADV‑5 Hexon protein, respectively, and are suitable for a double-antibody sandwich ELISA detection system. Among them, 8B9 is used as a coating antibody, and 4A6 is used as an HRP-labeled detection antibody. The combination of the two can achieve high sensitivity and high specificity detection of HADV‑5 and replication-deficient recombinant type 5 adenovirus, and does not cross-react with other common adenovirus types. The present invention also provides the variable region amino acid sequence and nucleotide sequence of the above-mentioned antibody, as well as an ELISA detection method based on the antibody combination. The method is simple to operate, has good repeatability, and is suitable for the detection of type 5 adenovirus.

The invention relates to the technical field of microbiological detection and molecular biology, in particular to a specific detection primer for bifidobacterium longum subsp. Longum BBMN68 and application of the specific detection primer. The specific primer for detecting the bifidobacterium longum subsp.longum BBMN68, provided by the invention, comprises an upstream primer and a downstream primer, and nucleotide sequences are shown as SEQ ID NO.7-8. The invention also provides a kit for detecting the bifidobacterium longum subsp.longum BBMN68. The specific DNA fragment provided by the invention is a specific molecular marker of BBMN68, and the specific primer is designed aiming at the fragment sequence, so that the specific amplification of the strain can be realized, no cross reaction is caused to other related strains, the detection sensitivity is higher, and the detection requirements of samples with different concentrations can be met; the method can be used for specific detection and accurate quantification of the BBMN68 strain in yoghourt and other products, and has good practical application value.

The invention discloses a specific detection target PsRrp8 of phytophthora sojae, a DNA (deoxyribonucleic acid) sequence of the specific detection target PsRrp8 is shown as SEQ ID NO.1. The invention further provides a primer combination for detecting the phytophthora sojae, the primer combination comprises a forward primer and a reverse primer, the sequence of the forward primer PsRrp8-qPCR-F1 is shown as SEQ ID NO.2, and the sequence of the reverse primer PsRrp8-qPCR-R1 is shown as SEQ ID NO.3. The invention further provides a kit for detecting the phytophthora sojae. The lowest detectable DNA concentration of the detection primer combination provided by the invention on the phytophthora sojae (P.sojae) is 0.332 pg.L <-1 >, which indicates that the quantitative detection technology has high sensitivity. The method can be successfully applied to phytophthora detection of phytophthora root rot disease field plants, phytophthora sojae can be rapidly detected, and a new technical means is provided for early warning of diseases.

The invention discloses a kit for detecting miRNAs of multiple extracellular vesicles based on a TtAgo-driven rolling circle amplification technology and application of the kit, a platform combines TtAgo protein and RCA reaction, cooperates with endonuclease activity and DNA polymerase activity of TtAgo, accurately controls reaction kinetic balance between temperature and enzyme activity, not only does not damage the cutting activity of TtAgo, but also can detect miRNAs of multiple extracellular vesicles. The method provided by the invention can be used for detecting multiple extracellular vesicles miRNAs, ensures strong polymerization amplification performance, creates a strong one-step single-tube experiment, greatly simplifies the experimental process, shortens the detection time, realizes ultra-sensitive and specific detection of nucleic acid markers, and can be used for detecting multiple extracellular vesicles miRNAs.

The invention belongs to the field of fluorescence detection, and particularly relates to a ratio-type fluorescent nanoprobe for specifically detecting tetracycline antibiotics as well as a preparation method and an application method of the ratio-type fluorescent nanoprobe. The nano-probe is a europium modified zinc gallium germanate nano-material, and is prepared by amino modification, citric acid modification and europium modification of the zinc gallium germanate nano-material. The probe can be specifically combined with tetracycline antibiotics; the fluorescent probe has two different fluorescence emission intensities, so that interference can be effectively eliminated, and the detection accuracy is high. And the synthesis method is simple and low in cost. The ratio type fluorescent nanoprobe provided by the invention also can realize the change of different colors under an ultraviolet lamp, can realize quantitative visual detection of tetracycline by combining the RGB value and the tetracycline concentration change, and can realize rapid on-site detection of tetracycline in a sample without the help of a fluorescent instrument. The tetracycline fluorescent probe is stable in optical performance, not prone to photobleaching, high in tetracycline recognition capacity, wide in response range (0.2-70 [mu] M) and low in detection limit, and has remarkable industrial application potential.

The invention discloses a composition, a kit and a method for detecting a mitochondrial gene of sea eel. A primer probe composition targeting the D-LOOP gene of the sea eel mitochondria is designed, and the nucleotide sequences of the primer probe composition correspond to SEQ ID No.1-3. On the basis, the invention further constructs and optimizes a specific real-time fluorescent PCR detection method for the D-LOOP gene of the sea eel mitochondria. According to the method, a part of sequence of the D-LOOP gene of the mitochondria of the sea eel is taken as a target region, a primer and a TaqMan-MGB probe are designed in a targeted manner, and finally a set of rapid, sensitive and high-specificity detection system is established. The detection system can be effectively applied to adulteration and germplasm identification work of the sea eel, and has a very wide industrial application prospect.

The invention discloses an organelle-targeted fluorescent probe for specific detection of tumor cells as well as preparation and application of the organelle-targeted fluorescent probe, and the specific structural formula of the fluorescent probe is as follows: BCY-DP shows obvious specific targeting ability of tumor cell mitochondria and has been applied to ONOO-selective imaging in living cell mitochondria; results show that the BCY-DP has high selectivity on ONOO <-> in tumor cells, can successfully distinguish the ovarian cancer cells from normal ovarian cells, and can be used for diagnosis of cancer cells.

The invention discloses a network service fault detection method, device and system, electronic equipment and a storage medium. The core network management device obtains a detection service, constructs a detection task according to the detection service and distributes the detection task to at least one edge network device, the edge network device executes the detection task to obtain a detection result and reports the detection result to the core network management device, and the core network management device analyzes the detection result and sends the analysis result to the detection server. And generating a whole network service state report. A complex detection task is decomposed into a distributed architecture of core definition and edge execution, the core network management device is responsible for definition and management of complex service logic, the edge network device executes a specific detection task, the architecture of service and data separation greatly reduces the performance requirement for the edge network device, and the service and data separation efficiency is improved. Therefore, common network equipment can complete complex service detection, high cost of special high-performance probe equipment is avoided, and hardware investment is remarkably reduced.

This invention discloses primer compositions, detection products, and applications for detecting 17 pathogens, relating to the field of biotechnology. A primer composition for detecting 17 pathogens includes 17 pairs of amplification primers and 17 extension probes, as shown in SEQ ID NO: 1-51, respectively. This invention also provides detection products comprising the above primer compositions and their application in simultaneously detecting 17 pathogens for non-disease diagnostic purposes. The beneficial effects of this invention are that it designs 17 primer sets to simultaneously amplify DNA fragments containing specific target sequences of 17 pathogens in multiplex PCR, and performs single-base extension of these products using highly efficient single-base primers. Simultaneous detection and identification of 17 pathogens can be achieved using MALDI-TOF MS mass spectrometry, with accurate and highly specific detection results.

The invention discloses an anti-deformation detection device for special equipment, and relates to the technical field of detection devices.The anti-deformation detection device comprises a box body, a base is arranged at the bottom end of the box body, guide portal frames are arranged at the top ends of the bases on the two sides of the box body, trusses are movably connected into the guide portal frames, and rear extension frames are arranged at the rear ends of the trusses; and a rotating shaft is arranged at the bottom of the rear extension frame. By arranging a series of structures, the detection range of the anti-deformation detection device for the special equipment is wider, multiple detection effects can be achieved without using multiple devices in cooperation, the cost is reduced, the anti-deformation detection device for the special equipment is convenient to detect equipment of different sizes, materials do not need to be cut into preset specifications, and the detection efficiency is improved. The detection efficiency is improved, the deformation resistance detection device for the special equipment can detect the deformation behavior of the material in a variable-temperature environment, and specific detection can be conveniently carried out on the materials in different use environments.

The invention provides a deployment method, device and system for a specific detection model in a medical scene, is used for carrying out localized deployment on a mature medical examination model in a hospital, specifically combines a container deployment technology and a container arrangement technology, provides corresponding model calling services for medical personnel more conveniently, efficiently and safely, and improves the medical examination efficiency. Dual requirements of data security compliance and model real-time calling can be effectively balanced, a safe, extensible and high-reliability operation platform is provided for actual landing of a digital algorithm, external dependence is greatly reduced, and the real-time performance of the digital algorithm is improved. The method provides possibility for rapid verification and iterative algorithm of hospital informatization departments and researchers in a clinical environment, achieves intelligent medical assistance of closed-loop, safe, controllable and efficient optimization in a hospital, has large-scale popularization value, and provides robust AI technical support for various clinical diagnosis, inspection analysis and scientific research work.

The utility model discloses a water hammer detection V-shaped ball valve, belongs to the technical field of ball valves, and solves the problem that specific detection needs to be carried out aiming at the situation that a water hammer phenomenon still occurs due to closing of a ball body in the V-shaped ball valve. Comprising a valve body, a water hammer detection circuit arranged on the valve body, a switch circuit coupled with the water hammer detection circuit and a buzzer coupled with the switch circuit, and the water hammer detection circuit is used for collecting the flow change in the valve body and collecting the vibration condition of the valve body to judge the result and control the switch circuit. The switching circuit is used for controlling the buzzer to be powered on and powered off according to the judgment result of the water hammer detection circuit. The flow sensor detects the flow of the outlet of the valve body, feeds back signals to the voltage comparison circuit, judges the flow change through delay signals, determines the water hammer phenomenon, triggers the 555 timing chip, controls the buzzer to give an alarm and reminds maintenance by combining the signals of the vibration sensor.

Systems and methods for multi-modality anomaly detection using artificial intelligence models such as fused models. Metric data and log data obtained from a monitored entity can be encoded into metric representations and log representations by utilizing transformer encoders of a cross-joint variational autoencoder (CJVAE). The metric representations and the log representations can be fused into a joint context representation by utilizing a fusion transformer encoder of the CJVAE. The joint context representation can be decoded by utilizing transformer decoders of the CJVAE to reconstruct the metric representations and the log representations. An anomaly for the monitored entity can be detected by aggregating detection results from the CJVAE based on the metric representations and the log representations, a metric-specific detection result from a metric detector, and a log-specific detection result from a log detector to resolve determined issues of the monitored entity caused by the anomaly.

The invention discloses a divalent nucleic acid aptamer fluorescence detection method based on an exonuclease I auxiliary target circulation strategy, and belongs to the field of analysis and detection.The divalent nucleic acid aptamer is a repetitive sequence composed of two monovalent nucleic acid aptamers and can form double-stranded DNA through base complementary pairing with complementary strand cDNA of the divalent nucleic acid aptamer, and the divalent nucleic acid aptamer can be used for fluorescence detection of the divalent nucleic acid aptamer. The method comprises the following steps: taking a bivalent nucleic acid aptamer as a template, forming a DNA silver nanocluster (DNA-AgNCs) under the action of silver nitrate and sodium borohydride, and when the target okadaic acid exists, combining the bivalent nucleic acid aptamer with the target, so that cDNA can be replaced from double-stranded DNA by the target. Exonuclease I is added to perform enzyme digestion on the cDNA and the divalent nucleic acid aptamer, so that the fluorescence intensity of the DNA-AgNCs can be rapidly reduced. The content of the okadaic acid can be judged by detecting the change of the fluorescence intensity. The invention provides a simple, convenient, rapid, high-sensitivity and high-specificity detection method for okadaic acid detection based on a divalent nucleic acid aptamer for the first time.

The invention relates to the technical field of power system state monitoring, and discloses an insulator defect detection method and device and a storage medium, and the method comprises the steps: collecting the leakage current of an insulator, and constructing a polarity conduction screening model through power frequency phase information, so as to generate a composite current sequence which highlights the polarization attribute of an interface; analyzing the nonlinear degree of the attenuation rate of the sequence drifting along with evolution time in a multi-stage diffusion evolution time domain, and generating an interface electrochemical mass transfer composite index; utilizing the index to invert and construct an interface polarization background reference and stripping the interface polarization background reference from the composite current sequence so as to extract an abnormal discharge pulse residual error; and finally, quantizing the pulse form distortion degree of the residual error, and performing suppression normalization operation on the distortion degree by using the index to output a judgment result. According to the invention, the interference of the interface mass transfer process is effectively eliminated, and the specific detection of the pollution flashover hidden danger is realized.

The invention provides a preparation method of an artemisia capillaris decoction reference sample. The invention also provides a construction method of the high performance liquid characteristic chromatogram of the artemisia capillaris decoction reference sample. According to the invention, the characteristic chromatogram of the artemisia capillaris decoction is constructed by adopting the high performance liquid chromatography for the first time, the blank of quality control of the artemisia capillaris decoction is filled through the method of the characteristic chromatogram, meanwhile, the quality condition of the prescription is comprehensively reflected, the quality control of the artemisia capillaris decoction is more perfect, and a means is added for the quality standard aspect of the artemisia capillaris decoction. According to the method disclosed by the invention, gradient elution is adopted for determination by adopting a high-performance liquid chromatography, and the method has the advantages of simplicity in operation, good separation degree, high precision, good repeatability, good stability and the like. Under specific detection conditions, the identification of the artemisia capillaris decoction characteristic spectrum method can be realized by taking a chlorogenic acid chromatographic peak as a reference substance S peak.

The invention discloses a sheep paratuberculosis specific detection kit and application, and relates to the field of biological detection.The kit comprises an elisa plate coated with a recombinant Mycobacterium paratuberculosis membrane protein antigen, the nucleotide sequence of the recombinant Mycobacterium paratuberculosis membrane protein antigen is shown as SEQ ID NO: 1, and the amino acid sequence of the recombinant Mycobacterium paratuberculosis membrane protein antigen is shown as SEQ ID NO: 2; a confining liquid; a serum diluent; carrying out enzyme labeling on a second antibody; a TMB color developing solution and a stop solution; according to the sheep paratuberculosis specific detection kit disclosed by the invention, the recombinant Mycobacterium paratuberculosis membrane protein antigen is adopted as a core detection component, and the antigen has high specificity and can accurately identify paratuberculosis positive serum and effectively distinguish positive and negative samples; meanwhile, prokaryotic expression and purification are carried out on the specific membrane protein of the mycobacterium paratuberculosis for the first time, and a novel sheep paratuberculosis indirect ELISA diagnosis method with high sensitivity and specificity based on the protein as an antigen is established.

The invention relates to an aptamer specifically combined with beta-lactoglobulin and application of the aptamer, and belongs to the technical field of biological detection. According to the aptamer and the preparation method thereof, a binding domain base, participating in recognition, of the aptamer is predicted through molecular docking firstly, then the binding domain base is subjected to directional mutation, the aptamer with the recognition performance improved is obtained, the affinity of the aptamer to beta-lactoglobulin is 10.6 nM, and the aptamer has no recognition capacity on alpha-lactalbumin, casein, bovine serum albumin, immune globulin G, lactose and the like and can be used for preparing the aptamer with the recognition performance improved. Therefore, the aptamer disclosed by the invention has good sensitivity and specificity on the beta-lactoglobulin. On the basis, a fluorescence polarization method is directly constructed by utilizing the characteristic that the beta-lactoglobulin is a biomacromolecule, when the beta-lactoglobulin exists, an aptamer marked by a fluorophore FAM recognizes the beta-lactoglobulin to form an aptamer beta-lactoglobulin compound, and the fluorescence polarization value is relatively large, so that the beta-lactoglobulin compound can be used for identifying the beta-lactoglobulin. Therefore, the specific detection of the low-concentration beta-lactoglobulin is realized.

The disclosure relates to method and kits for highly specific detection and quantification of extracellular vesicles (EVs) by targeting at least two EV surface markers using binding reagents. The binding reagents contain hybridisation sequences and primer binding regions, the hybridisation sequences are ligated and amplified using primers. The disclosure further relates to methods and kits for isolating EVs using capture entities, splint entities and staple entities wherein the capture entity comprises a labile linkage.

The invention relates to the technical field of intelligent defect detection, in particular to a strain clamp defect detection method and system based on unmanned aerial vehicle digital X-rays. The method comprises the steps of obtaining target equipment information, and obtaining a detection strategy parameter according to the target equipment information; an imaging control parameter is generated by acquiring spatial positioning data and combining detection strategy parameters, a detection area is divided into a plurality of scanning subareas, and a special detection angle, hovering time and an image overlapping rate are set for each subarea; the defect detection result is subjected to confidence analysis, the detection confidence is calculated, when the detection confidence is lower than an acceptable range, a detection optimization adjustment instruction can be automatically triggered, self-adaptive adjustment is executed according to compensation control information, and detection parameters and a detection process are dynamically optimized; and the detection precision, reliability and adaptability are improved.

The invention provides a primer probe set for digital PCR detection of non-small cell lung cancer EGFR mutation and application thereof, and relates to the technical field of digital PCR detection, the primer probe set comprises a primer pair and a probe for specific detection of EGFR gene exon 19 deletion mutation (19del) and exon 21 L858R point mutation, and the 19del mutation probe comprises a plurality of sequences to cover common subtypes. The primer probe group can be combined with a microfluidic digital PCR platform for use, a sample is divided into tens of thousands of microdroplets for amplification, and FAM, HEX, ROX and CY5 fluorescence channel signals are detected to realize absolute quantification. The system provided by the invention has high sensitivity and excellent linearity and precision, is particularly suitable for detecting extremely-low-frequency EGFR mutation in body fluid such as plasma, and has important application value in individualized diagnosis and treatment, curative effect monitoring and drug resistance evaluation of non-small cell lung cancer.

This invention discloses a near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe, its preparation method, and its application, belonging to the field of medical detection technology. The structural formula of the near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe is as follows: The near-infrared two-photon dual-mode linked response NTR&Cys fluorescent probe of this invention possesses a hypoxia dual-mode visual monitoring molecular framework, enabling dual-mode determination of hypoxia microenvironments. It improves the stability of the cyanine near-infrared framework and achieves highly stable and specific detection of nitroreductase under hypoxia. Under hypoxia conditions, the product reacts with NTR, and the single-photon excited near-infrared fluorescence is quenched and then illuminated. Simultaneously, under hypoxia, the probe reacts with Cys to generate a two-photon excited fluorescence signal, effectively avoiding the probe consumption problem of dual-response systems and ensuring the non-interference of fluorescence excitation and emission (dual-mode), thus truly achieving accurate locking of hypoxia microenvironments.

The invention provides a routing state sensing method and device for distributed network probe cooperative monitoring, and relates to the technical field of communication network monitoring, and the method comprises the steps: deploying a distributed probe device at a key node, and integrating an active detection module, a passive flow monitoring module, a data fusion analysis unit and a detection control module; the active detection module supports periodic detection and specified detection, and dynamically adjusts the detection priority and path; the passive monitoring module realizes fault positioning and anomaly tracing based on deep packet detection and a machine learning model; the data fusion analysis unit constructs a dynamic routing state map through a space-time association algorithm, and depicts the link health degree and the abnormal situation; the detection control module dynamically switches detection modes according to network load, resource consumption and historical indexes, and balances detection precision and overhead. According to the invention, through active and passive cooperative detection and an intelligent decision-making mechanism, the network sensing precision is improved, the resource consumption is reduced, and efficient autonomous operation and maintenance of a 5G / 6G network are supported.

The invention discloses a method for detecting glyphosate by using a copper monatomic coordination amino covalent organic framework nano-enzyme, the copper monatomic coordination amino covalent organic framework nano-enzyme adopted in the method comprises an amino covalent organic framework, and Cu < 2 + > ions are distributed on a cavity and the surface of the amino covalent organic framework. The nano-enzyme adopted in the invention has the advantages of excellent peroxidase-like activity, high crystallinity, strong chemical stability and the like, can realize specific detection of glyphosate, has the advantages of simple process, convenience in operation, low cost, wide detection range, high detection sensitivity, high detection precision and the like, has the glyphosate detection concentration of 2-100 [mu] mol / L, and has a good application prospect. The detection limit is 0.193 mu mol / L, the method can be widely used for detecting glyphosate in the environment (such as soil or water), the concentration of glyphosate can be rapidly and accurately obtained, the use value is high, and the application prospect is good.

The invention discloses a method and system for analyzing next-generation sequencing data and application. The specific detection of the transcriptional activity of the cccDNA is realized by utilizing the two characteristic sequences, a new analysis method is provided for evaluating the antiviral treatment effect and judging functional cure, and individualized treatment and new drug research and development can be guided. In addition, the method is also suitable for designing specific quantitative PCR probes and primers, the cccDNA transcriptional level is evaluated by detecting characteristic fragments, and a more convenient detection means is provided for clinical laboratories.

The invention belongs to the technical field of biology. Specifically, the invention provides a porcine epidemic diarrhea virus micro-fluidic fluorescent RT-PCR detection kit and application thereof based on a micro-fluidic card slot type chip technology, the detection kit comprises a broad-spectrum primer probe which covers almost all PEDV strains and still has high sensitivity in the detection kit, and meanwhile, a proper exogenous internal standard system is introduced. The exogenous internal standard sequence is specifically designed, is compatible with PEDV target amplification conditions and does not interfere with the PEDV target amplification conditions, efficient, sensitive and specific detection of PEDV pathogens is achieved, the detection sensitivity is as low as 4.5 * 10 < 2 > copy / ml, and no cross reaction exists on six other pathogens.

The invention relates to the technical field of biological detection, and discloses a method for double-ring detection of lung cancer L858R mutation based on CRISPR / Cas9 and DNA, and the method comprises the following steps: enriching circulating tumor cells CTC from a blood sample; extracting genomic DNA (deoxyribonucleic acid) of the enriched CTC; carrying out amplification on the DNA fragment containing the L858R mutation by adopting recombinase polymerase amplification RPA (recombinase polymerase amplification); the amplified DNA fragment is hybridized with a recognition ring in a DNA double-ring structure, a DNA double ring is composed of the recognition ring and a report ring, and a part of region of the recognition ring is hybridized with a corresponding fragment of the report ring to form a staggered double-chain region; when L858R mutation exists, the mutated DNA and a recognition ring are hybridized to activate a CRISPR / Cas9 system, and under the guidance of sgRNA, Cas9 cuts hybrid double strands and releases a report ring; carrying out rolling circle amplification RCA under the action of DNA polymerase by taking the released report ring as a template; a fluorescent probe is used for detecting an RCA amplification product, and whether L858R mutation exists or not is judged according to a fluorescence signal. The CRISPR / Cas9 system is combined with a DNA double-ring structure, so that the ultra-sensitive and specific detection on the lung cancer L858R mutation is realized.