331 results about "Specific detection" patented technology

The invention relates to the technical field of microbiological detection and molecular biology, in particular to a specific detection primer for bifidobacterium longum subsp. Longum BBMN68 and application of the specific detection primer. The specific primer for detecting the bifidobacterium longum subsp.longum BBMN68, provided by the invention, comprises an upstream primer and a downstream primer, and nucleotide sequences are shown as SEQ ID NO.7-8. The invention also provides a kit for detecting the bifidobacterium longum subsp.longum BBMN68. The specific DNA fragment provided by the invention is a specific molecular marker of BBMN68, and the specific primer is designed aiming at the fragment sequence, so that the specific amplification of the strain can be realized, no cross reaction is caused to other related strains, the detection sensitivity is higher, and the detection requirements of samples with different concentrations can be met; the method can be used for specific detection and accurate quantification of the BBMN68 strain in yoghourt and other products, and has good practical application value.

The invention discloses a kit for detecting miRNAs of multiple extracellular vesicles based on a TtAgo-driven rolling circle amplification technology and application of the kit, a platform combines TtAgo protein and RCA reaction, cooperates with endonuclease activity and DNA polymerase activity of TtAgo, accurately controls reaction kinetic balance between temperature and enzyme activity, not only does not damage the cutting activity of TtAgo, but also can detect miRNAs of multiple extracellular vesicles. The method provided by the invention can be used for detecting multiple extracellular vesicles miRNAs, ensures strong polymerization amplification performance, creates a strong one-step single-tube experiment, greatly simplifies the experimental process, shortens the detection time, realizes ultra-sensitive and specific detection of nucleic acid markers, and can be used for detecting multiple extracellular vesicles miRNAs.

The invention discloses a composition, a kit and a method for detecting a mitochondrial gene of sea eel. A primer probe composition targeting the D-LOOP gene of the sea eel mitochondria is designed, and the nucleotide sequences of the primer probe composition correspond to SEQ ID No.1-3. On the basis, the invention further constructs and optimizes a specific real-time fluorescent PCR detection method for the D-LOOP gene of the sea eel mitochondria. According to the method, a part of sequence of the D-LOOP gene of the mitochondria of the sea eel is taken as a target region, a primer and a TaqMan-MGB probe are designed in a targeted manner, and finally a set of rapid, sensitive and high-specificity detection system is established. The detection system can be effectively applied to adulteration and germplasm identification work of the sea eel, and has a very wide industrial application prospect.

The invention discloses a network service fault detection method, device and system, electronic equipment and a storage medium. The core network management device obtains a detection service, constructs a detection task according to the detection service and distributes the detection task to at least one edge network device, the edge network device executes the detection task to obtain a detection result and reports the detection result to the core network management device, and the core network management device analyzes the detection result and sends the analysis result to the detection server. And generating a whole network service state report. A complex detection task is decomposed into a distributed architecture of core definition and edge execution, the core network management device is responsible for definition and management of complex service logic, the edge network device executes a specific detection task, the architecture of service and data separation greatly reduces the performance requirement for the edge network device, and the service and data separation efficiency is improved. Therefore, common network equipment can complete complex service detection, high cost of special high-performance probe equipment is avoided, and hardware investment is remarkably reduced.

This invention discloses primer compositions, detection products, and applications for detecting 17 pathogens, relating to the field of biotechnology. A primer composition for detecting 17 pathogens includes 17 pairs of amplification primers and 17 extension probes, as shown in SEQ ID NO: 1-51, respectively. This invention also provides detection products comprising the above primer compositions and their application in simultaneously detecting 17 pathogens for non-disease diagnostic purposes. The beneficial effects of this invention are that it designs 17 primer sets to simultaneously amplify DNA fragments containing specific target sequences of 17 pathogens in multiplex PCR, and performs single-base extension of these products using highly efficient single-base primers. Simultaneous detection and identification of 17 pathogens can be achieved using MALDI-TOF MS mass spectrometry, with accurate and highly specific detection results.

Systems and methods for multi-modality anomaly detection using artificial intelligence models such as fused models. Metric data and log data obtained from a monitored entity can be encoded into metric representations and log representations by utilizing transformer encoders of a cross-joint variational autoencoder (CJVAE). The metric representations and the log representations can be fused into a joint context representation by utilizing a fusion transformer encoder of the CJVAE. The joint context representation can be decoded by utilizing transformer decoders of the CJVAE to reconstruct the metric representations and the log representations. An anomaly for the monitored entity can be detected by aggregating detection results from the CJVAE based on the metric representations and the log representations, a metric-specific detection result from a metric detector, and a log-specific detection result from a log detector to resolve determined issues of the monitored entity caused by the anomaly.

The invention discloses a divalent nucleic acid aptamer fluorescence detection method based on an exonuclease I auxiliary target circulation strategy, and belongs to the field of analysis and detection.The divalent nucleic acid aptamer is a repetitive sequence composed of two monovalent nucleic acid aptamers and can form double-stranded DNA through base complementary pairing with complementary strand cDNA of the divalent nucleic acid aptamer, and the divalent nucleic acid aptamer can be used for fluorescence detection of the divalent nucleic acid aptamer. The method comprises the following steps: taking a bivalent nucleic acid aptamer as a template, forming a DNA silver nanocluster (DNA-AgNCs) under the action of silver nitrate and sodium borohydride, and when the target okadaic acid exists, combining the bivalent nucleic acid aptamer with the target, so that cDNA can be replaced from double-stranded DNA by the target. Exonuclease I is added to perform enzyme digestion on the cDNA and the divalent nucleic acid aptamer, so that the fluorescence intensity of the DNA-AgNCs can be rapidly reduced. The content of the okadaic acid can be judged by detecting the change of the fluorescence intensity. The invention provides a simple, convenient, rapid, high-sensitivity and high-specificity detection method for okadaic acid detection based on a divalent nucleic acid aptamer for the first time.

The invention relates to the technical field of power system state monitoring, and discloses an insulator defect detection method and device and a storage medium, and the method comprises the steps: collecting the leakage current of an insulator, and constructing a polarity conduction screening model through power frequency phase information, so as to generate a composite current sequence which highlights the polarization attribute of an interface; analyzing the nonlinear degree of the attenuation rate of the sequence drifting along with evolution time in a multi-stage diffusion evolution time domain, and generating an interface electrochemical mass transfer composite index; utilizing the index to invert and construct an interface polarization background reference and stripping the interface polarization background reference from the composite current sequence so as to extract an abnormal discharge pulse residual error; and finally, quantizing the pulse form distortion degree of the residual error, and performing suppression normalization operation on the distortion degree by using the index to output a judgment result. According to the invention, the interference of the interface mass transfer process is effectively eliminated, and the specific detection of the pollution flashover hidden danger is realized.

The invention relates to an aptamer specifically combined with beta-lactoglobulin and application of the aptamer, and belongs to the technical field of biological detection. According to the aptamer and the preparation method thereof, a binding domain base, participating in recognition, of the aptamer is predicted through molecular docking firstly, then the binding domain base is subjected to directional mutation, the aptamer with the recognition performance improved is obtained, the affinity of the aptamer to beta-lactoglobulin is 10.6 nM, and the aptamer has no recognition capacity on alpha-lactalbumin, casein, bovine serum albumin, immune globulin G, lactose and the like and can be used for preparing the aptamer with the recognition performance improved. Therefore, the aptamer disclosed by the invention has good sensitivity and specificity on the beta-lactoglobulin. On the basis, a fluorescence polarization method is directly constructed by utilizing the characteristic that the beta-lactoglobulin is a biomacromolecule, when the beta-lactoglobulin exists, an aptamer marked by a fluorophore FAM recognizes the beta-lactoglobulin to form an aptamer beta-lactoglobulin compound, and the fluorescence polarization value is relatively large, so that the beta-lactoglobulin compound can be used for identifying the beta-lactoglobulin. Therefore, the specific detection of the low-concentration beta-lactoglobulin is realized.

The invention provides a primer probe set for digital PCR detection of non-small cell lung cancer EGFR mutation and application thereof, and relates to the technical field of digital PCR detection, the primer probe set comprises a primer pair and a probe for specific detection of EGFR gene exon 19 deletion mutation (19del) and exon 21 L858R point mutation, and the 19del mutation probe comprises a plurality of sequences to cover common subtypes. The primer probe group can be combined with a microfluidic digital PCR platform for use, a sample is divided into tens of thousands of microdroplets for amplification, and FAM, HEX, ROX and CY5 fluorescence channel signals are detected to realize absolute quantification. The system provided by the invention has high sensitivity and excellent linearity and precision, is particularly suitable for detecting extremely-low-frequency EGFR mutation in body fluid such as plasma, and has important application value in individualized diagnosis and treatment, curative effect monitoring and drug resistance evaluation of non-small cell lung cancer.

This invention provides a CRISPR / Cas12a fluorescence detection method for FocTR4 based on magnetic bead-ssDNA-HRP probes, belonging to the field of nucleic acid detection technology. This invention involves isothermal amplification of the FocTR4 target sequence using RPA primers; then, a magnetic bead-ssDNA-HRP probe is prepared; and the Cas12a / crRNA complex, RPA amplification product, and probe are co-incubated. The activated Cas12a cleaves the probe, releasing HRP. After magnetic separation, a colorimetric solution containing OPD is added, and the presence and concentration of FocTR4 are determined by detecting the fluorescence signal. This invention combines the high efficiency of CRISPR / Cas12a cleavage with the high sensitivity and strong specificity of RPA technology, achieving highly sensitive and specific detection of FocTR4, with a linear detection range of 10. 2 ~10 7 With a detection limit as low as 70 CFU / mL, it can quickly and accurately detect Foc TR4 in soil samples, enabling rapid on-site detection and providing reliable technical support for the early diagnosis and control of banana wilt disease.

The application belongs to the technical field of microorganisms, and discloses nucleic acid, a kit and a detection method for multiplex real-time fluorescent quantitative RT-PCR detection of group A rotavirus genotyping. The detected genotypes include G1, G2, G3, G4, G8, G9, P[4], P[6] and P[8], and grouping detection is performed, at least two genotypes are detected in each group, and preferably three groups are detected, wherein G1, G9 and P[6] are group A, G2, G4 and P[4] are group B, and G3, G8 and P[8] are group C. The method can quickly and efficiently detect nine genotypes of group A rotavirus, can effectively save rare samples, and can save reagents, has high sensitivity and specificity, the result is simple to read and easy to apply, and provides a reference for specific detection of group A rotavirus typing and prevention and control of group A rotavirus.

This invention discloses nucleic acid aptamers that specifically bind to the OmpK36 protein and their applications. Through analysis and screening, aptamers APT-1 and APT-2 that specifically bind to the OmpK36 protein were obtained. Nucleic acid aptamer APT-1 can specifically bind to outer membrane polypeptides, while nucleic acid aptamer APT-2 can specifically bind to polypeptides specific to Klebsiella pneumoniae. Both can bind to the OmpK36 protein to form a complex structure, which can be used for the specific detection of Klebsiella pneumoniae, providing convenience for clinical detection, diagnosis and treatment, and exclusion detection of specific pathogens in SPF animals.

The invention discloses an Au (at) PtNCs nano-enzyme probe for detecting novel nicotine pesticides as well as a preparation method and application of the Au (at) PtNCs nano-enzyme probe, and belongs to the field of rapid detection of biosensors. The invention provides an Au (at) PtNCs nano-enzyme probe, which is generated by combining AuNCs and PtNCs and coupling with a second antibody, and can effectively realize rapid specific detection of novel nicotine pesticides such as imidacloprid and the like. The linear range of the biosensor probe for detecting the imidacloprid pesticide is 0.1-100 [mu] g / L, and the limit of detection (LOD) can reach 0.127 [mu] g / L and 0.119 [mu] g / L respectively by combining a colorimetric method and a fluorescence method.

This invention belongs to the field of molecular biology detection technology, specifically relating to a primer and probe combination for detecting avian Salmonella based on the RAA-LFD method and its application, particularly to a primer and probe combination for detecting three avian serotypes of Salmonella: Salmonella pullorum, Salmonella enteritidis, and Salmonella typhimurium, based on the RAA-LFD method and its application. This invention designs different types of probes and primers targeting the genes of serotypes SP, SE, and STM of Salmonella. Using the primer and probe combination of this invention, rapid, sensitive, and specific detection of Salmonella serotypes and the three avian Salmonella serotypes SP, SE, and STM can be achieved, greatly shortening the detection cycle of avian salmonellosis and improving the detection efficiency and accuracy of avian salmonellosis.

This invention relates to monoclonal antibodies for detecting A35R protein and their applications. This invention screened six antibodies, including antibody 4E7, antibody 11G12, antibody 6F10, antibody 6C11, antibody 18C3, and antibody 23A7. Specifically, this invention discloses the amino acid sequences of the heavy chain variable region and the light chain variable region of the above antibodies, as well as the amino acid sequences of their complementarity-determining regions and framework regions. Antibodies 4E7 and 11G12 can bind not only to the A35R-MPXV protein but also to its homologs A35R-CPXV, A35R-VTT8, and A35R-Variola. Antibodies 6F10, 6C11, 23A7, and 18C3 can specifically recognize the A35R-MPXV protein. The monoclonal antibodies described above all exhibit high binding capacity to the A35R protein, making them suitable for the sensitive and specific detection and prevention of monkeypox virus A35R protein. They can also be used clinically for the diagnosis and treatment of monkeypox virus infection-related diseases.

The invention provides a primer for detecting a novel bunyavirus by an RPA-lateral flow nucleic acid chromatography technology, which comprises a specific SFTSV primer pair marked by a nucleic acid sequence, namely SFTSV-F and SFTSV-R. The invention further provides a kit for detecting the novel bunyavirus by the RPA-lateral flow nucleic acid chromatography technology. The invention also provides a probe which comprises a gold nanoparticle capture probe, an SFTSV capture probe and a quality control capture probe. Meanwhile, the invention further provides application of the primer and the probe in preparation of SFTSV detection products, a specific detection kit and a specific detection method. The specific primer and the functional differentiation probe are designed aiming at a novel bunyavirus highly-conserved sequence, and an RPA isothermal amplification technology and a lateral flow nucleic acid chromatography visual detection technology are integrated, so that the rapid, sensitive and specific detection of the SFTSV is realized, the dependence on a large instrument is not needed, the detection cost and the operation threshold are reduced, and the detection efficiency is improved. And the application requirements of on-site rapid detection, large-scale screening and resource-limited regions are met.

The invention discloses a cascade seven-core optical fiber sensor applied to folic acid detection and a preparation method of the cascade seven-core optical fiber sensor, and belongs to the technical field of optical fiber sensing and biological analysis detection. The sensor comprises a first single-mode optical fiber, a seven-core optical fiber section and a second single-mode optical fiber, a double-microsphere-cavity structure is arranged at the optical fiber connecting position to form a cascade interference optical path, and tapering is conducted on the middle portion of the seven-core optical fiber section to form a sensing area. According to the invention, mode matching is improved through the microsphere cavity, an evanescent field is enhanced through tapering, and high-sensitivity specific detection of folic acid is realized in cooperation with the folic acid binding protein biological functional layer fixed on the surface; the sensor obtains stable linear response to a folic acid standard solution in the range of 0-50 ng / mL, a clinical serum sample verifies that the detection result of the sensor is highly consistent with that of a chemiluminescence method, the sensor has good reusability, and the sensor has the advantages of being easy to prepare in structure, rapid in response, high in sensitivity, capable of being matched with a complex matrix and the like, and has good application prospects. The method has huge potential in clinical folic acid quantitative detection.

The invention discloses a fluorescent probe for beta-lactoglobulin detection and a preparation method and application thereof, and relates to the technical field of food detection. The structure of the fluorescent probe is shown in the specification, r1 and R2 are independently selected from hydrogen or C1-C5 alkoxy, and R3 is methyl or ethyl. Aggregation-induced emission (AIE) is combined with inhibition of twisted intramolecular charge transfer (TICT) when a beta-lactoglobulin (beta-LG) cavity is inserted, a D-pi-A skeleton is adopted, triphenylamine is used as a conservative electron donor and a conservative molecular rotor, and cavity coordination and static electricity are optimized by repositioning receptor connection, so that ICT and charge distribution are systematically adjusted. The cavity locking strategy integrates molecular recognition and motion limitation, and can realize rapid analysis and specific detection of beta-LG in a complex dairy product matrix.

The present application relates to the technical field of analytical detection, and in particular to a detection method of Geobacillus thermophilus in raw milk based on real-time fluorescent quantitative polymerase chain reaction. The detection method of the present application is based on nucleic acid specific detection mechanism, which replaces the phenotype culture mechanism, realizes the rapid and accurate quantification of the target bacteria, and replaces the time-consuming and inaccurate colony counting. Through the design of specific primers and probes, the species level specificity identification is realized, and the problem that the traditional method cannot distinguish the specific contaminated bacteria is solved. Through the optimization of the pretreatment method, the sensitivity of the detection method is greatly improved. The detection method of the present application shortens the detection time, realizes the leap from "days" to "hours", greatly improves the detection efficiency, and has a wide application prospect.

The application discloses a method for detecting polychlorinated biphenyl by using an electrochemical aptamer sensor. x The sensor comprises a glassy carbon electrode, a surface of a detection end of the glassy carbon electrode is decorated with an electroactive nanomaterial, the electroactive nanomaterial comprises Ti3C2T 0000001 Nanosheet, a conductive polymer material is embedded in an interlayer structure of the nanosheet and forms a composite nanomaterial, and gold nanoparticles are deposited on a surface of the composite nanomaterial; a capture chain is self-assembled on the electroactive nanomaterial, a specific aptamer probe is combined on the capture chain, a DNA double-stranded structure is formed, and an electroactive label is introduced. In the application, the sensor has the advantages of wide detection range, low detection limit, strong anti-interference ability, good stability and the like, can realize specific detection of polychlorinated biphenyl, has the advantages of low cost, short time consumption, high accuracy, good adaptability and the like, and has important significance for rapid detection of polychlorinated biphenyl.

This invention relates to a pyridoxal phosphate-responsive biosensor and its application, belonging to the field of synthetic biology biosensor technology. This invention is the first to discover and identify the relationship between pyridoxal phosphate and Cgl1185-P. cgl2838 The transcriptional regulation and response relationship between these factors was investigated, and this relationship was used as the basis for constructing a biosensor responsive to pyridoxal. The transcription factor regulatory system Cgl1185-P derived from Corynebacterium glutamicum was used. cgl2838 A biosensor for the specific detection of pyridoxal phosphate was constructed by assembling a reporter gene as the main component onto a plasmid, enabling effective detection of pyridoxal phosphate concentrations in intracellular or culture environments. This biosensor possesses advantages such as clear structure, high sensitivity, and good host adaptability, and can be used for fermentation process monitoring, cofactor metabolism research, and screening of relevant strains, showing promising application prospects in the fields of cofactor concentration monitoring and metabolic regulation.

This invention provides a specific detection method for *Daphnia magna* in nearshore marine aquatic ecosystem assessment. Based on obtaining the species-specific antigenic protein and key immunogenic peptides of *Daphnia magna*, a highly specific monoclonal antibody was successfully prepared and purified, and a colloidal gold side-flow chromatography rapid detection test strip was prepared using this antibody. The amino acid sequence of the antigenic protein is SEQ ID NO:1 or SEQ ID NO:2. This invention, by screening for the *Daphnia magna* species-specific antigen and its corresponding monoclonal antibody, combined with paired antibodies prepared from the full-length protein, and using them to develop a colloidal gold rapid immunoassay product, achieves for the first time high-throughput, rapid on-site screening of this species, significantly improving the efficiency and accuracy of marine ecological monitoring, ship ballast organism inspection, and aquaculture environmental assessment.

The invention belongs to the technical field of phytopathogen detection, and particularly relates to a specific detection primer and a specific detection kit for Cercospora cf.flagellaris, and an application of the specific detection primer and the specific detection kit for Cercospora cf.flagellaris. By adopting the detection primer or the kit disclosed by the invention, the specific detection of the phytolacca acinosa can be realized, and the detection primer or the kit has good intraspecific strain universality. Meanwhile, through a sensitivity experiment, the detection system and the detection method established by the invention have the lowest detection concentration of 0.012 pg.L <-1 > aiming at the phytolacca acinosa. The detection system is used for detecting soybean grains with purple spot symptoms in the field, and can be used for quickly and accurately identifying whether soybean plants carry pathogenic bacteria of sporospora phytolacca or not. Therefore, the detection primer and the kit provided by the invention have good application prospects.

The invention provides an insulation fault detection system and method. The insulation fault detection system comprises a detection unit, a grounding subsystem, a leakage current detection subsystem and a controller. The detection unit is a maximum power point tracking circuit formed by photovoltaic group strings or parallel connection of the photovoltaic group strings. And the grounding subsystem is connected with the positive and negative electrodes of the direct-current bus and the ground and executes positive electrode or negative electrode grounding switching operation. The leakage current detection subsystems are correspondingly arranged on the detection units and used for collecting leakage current. The insulation fault detection system is configured to be capable of forming an independent measurement loop dominated by a current detected unit when executing leakage current collection, the controller receives leakage current values of all the units in sequence based on the independent loop, and through preset numerical value processing, a specific detection unit with an insulation fault can be positioned, and the leakage current values of all the units can be detected. And the approximate position range of the fault photovoltaic module in the detection unit can be further calculated.

The invention belongs to the technical field of biological detection, and relates to a Chikungunya virus detection kit and a detection method. The kit is based on the principle of PCR detection and comprises a primer pair, the sequence of a forward primer of the primer pair is shown as SEQ ID NO: 1, and the sequence of a reverse primer of the primer pair is shown as SEQ ID NO: 2. The chikungunya virus detection kit and the detection method can realize rapid, sensitive and specific detection of chikungunya virus based on the principle of PCR detection, and are especially suitable for various application scenes such as clinical samples, human or animal samples, environmental samples, scientific research samples and the like.

This invention discloses an early screening system and method for hyperuricemia based on fingertip blood uric acid detection. The system includes a fingertip blood collection module, a detection module, a data processing module, a storage module, an analysis module, an early warning module, a display module, a communication module, a calibration module, and a power supply module. It accurately collects fingertip blood samples from subjects, obtains blood uric acid concentration data using specific detection equipment, constructs a multi-dimensional analysis model based on clinical characteristic parameters, and determines the optimal diagnostic cutoff value based on ROC curves, achieving rapid and accurate screening for hyperuricemia. The method includes steps such as sample collection, detection, data processing, model analysis, result determination, and early warning. It is simple to operate, fast, and highly accurate, solving the problems of poor compliance and difficulty in grassroots implementation associated with traditional venous blood testing. It is suitable for large-scale population screening and early diagnosis of hyperuricemia in areas with scarce medical resources, and has significant clinical application value and social significance.

The invention provides a nucleic acid composition and a kit for identifying a rice variety rice flower No.2, and relates to the technical field of biochemical detection. The nucleic acid composition comprises a specific amplification primer pair and a specific detection probe. The nucleic acid composition locks the specific SNP site of the rice, and by utilizing the synergistic effect of the primer and the double probes, the rice flowery No.2 variety and the similar variety can be accurately distinguished, and the doping can be sensitively identified. The method is suitable for a real-time fluorescent PCR platform, electrophoresis and sequencing are not needed, rapid and high-sensitivity closed tube detection is achieved, and an efficient means is provided for authenticity identification and purity analysis.

Provided is an internal reference gene for the real-time quantitative PCR (RT-qPCR) of Yersinia enterocolitica, including one of or a combination of some of glnS, nuoB, glmS, gyrB, dnaK, and thrS genes. Further provided is a primer set for amplifying the internal reference gene for the RT-qPCR of Yersinia enterocolitica. Given that stable housekeeping genes of Yersinia enterocolitica remain unclear, internal reference genes with relatively stable expression at different culture temperatures are selected by screening for Yersinia enterocolitica. Specific detection primers and real and reliable data provide a reference basis for selecting housekeeping genes of Yersinia enterocolitica; moreover, the use thereof alone or in combination, as an internal reference gene, improves the data accuracy, stability, and reliability, thereby solving the problem that usually only empirically selecting 16s rRNA as a single internal reference gene in conventional RT-qPCR results in unstable research results.

The application discloses a colorimetric-SERS dual-mode detection and photothermal sterilization method for specifically detecting Vibrio parahaemolyticus and application thereof, and belongs to the technical field of food safety biology. The application takes Vibrio parahaemolyticus as a target, and prepares a multifunctional magnetic composite nanomaterial through layer-by-layer assembly. Firstly, the superparamagnetic separation performance of the magnetic composite nanomaterial is combined with magnetic chromatography to achieve the effect of rapidly separating bacteria from a complex food matrix. Secondly, the colorimetric and SERS sensing signals of the magnetic composite nanomaterial are used to quantitatively detect bacteria in a short time, high precision and high stability in a dual-mode. Finally, the photothermal performance of the magnetic composite nanomaterial is used to kill the detected bacteria by photothermal sterilization, so that the hazard control at the back end of detection is completed.