2115 results about "Specific detection" patented technology

This document describes an optical sensor unit and a procedure for the specific detection and identification of biomolecules at high sensitivity in real fluids and tissue homogenates. High detection limits are reached by the combination of i) label-free integrated optical detection of molecular interactions, ii) the use of specific bioconstituents for sensitive detection and iii) planar optical transducer surfaces appropriately engineered for suppression of non-specific binding, internal referencing and calibration. Applications include the detection of prion proteins and identification of those biomolecules which non-covalently interact with surface immobilized prion proteins and are intrinsically involved in the cause of prion related disease.

Methods, compositions and kits which employ one or more ZEB specific detection reagents for detection and localization of ZEB associated molecules in tumor cells are disclosed. Also provided are methods for determining stage and progression of cancer in a mammal based on alterations in ZEB expression levels and subcellular localization. Also provided are methods for treating a cancer in a mammal by modulating ZEB expression levels and activity.

The invention provides a method for genetic analysis in individuals that reveals both the genetic sequences and chromosomal copy number of targeted and specific genomic loci in a single assay. The present invention further provide methods for the sensitive and specific detection of target gene sequences and gene expression profiles.

Methods for enhancing the dynamic range for specific detection of one or more analytes in assays using scattered-light detectable particle labels. The methods involve utilizing variations in detection technique and/or signal processing to extend the dynamic range to either or both of lower and higher concentrations.

There is disclosed a sensitive method for specific detection of ligands comprising the steps of (a) contacting the sample with reagents capable of forming a reagent complex, which reagent complex comprises a receptor capable of specifically binding to said ligand and a nucleic acid molecule; and (b) detecting any complexes formed by binding of the receptor part of said reagent complex to ligand present in the sample by specifically detecting the presence of the nucleic acid molecule by amplifying a region of the nucleic acid and simultaneously detecting products of the amplification reaction in real-time.

A method for mapping surroundings of a vehicle, objects in the surroundings of the vehicle being detected with the aid of sensors and particular detected objects being described by two coordinate points and also by a position fuzziness assigned to the particular coordinate point, the coordinate points and the position fuzziness values being stored in an interface in the form of data which may be accessed by driver assistance systems of the vehicle. A method is also described for ascertaining the collision probability of a vehicle with an object, in which the surroundings of the vehicle are initially mapped using the method for mapping the surroundings of a vehicle, a travel path to be traveled by the vehicle is ascertained in a subsequent step, and the degree of overlap between the object and the travel path and also the collision probability are finally determined, taking the position fuzziness into account.

A method for correlating event information comprises receiving event information for a plurality of detected events wherein the event information for a particular detected event comprises a plurality of attributes associated with that detected event. The method continues by assigning a plurality of attribute values to each detected event, the attribute values of each detected event defining a point in n-dimensional space. The method continues by storing the event information for each detected event in accordance with the attribute values assigned to that detected event. The method continues by receiving a target event comprising a plurality of attributes wherein the attributes of the target event are associated with attribute values and the attribute values of the target event define a target point in n-dimensional space. The method continues by receiving a plurality of proximity limits that define a portion of n-dimensional space surrounding the target point. The method concludes by identifying a plurality of detected events wherein the points defined by the attribute values of the identified detected events are within the portion of n-dimensional space defined by the proximity limits.

The present invention provides a rapid and sensitive method for the detection of a West Nile virus (WNV), Japanese encephalitis virus (JEV), St. Louis encephalitis virus (SLEV) and Dengue virus (DENV) and antibodies directed against thereof involving contacting a biological specimen suspected of being infected with WNV, JE, SLE or DEN with a substantially purified and isolated WNV E glycoprotein or subfragment thereof having a native conformation wherein the E glycoprotein or subfragment thereof has a reactivity with antibodies against WNV and a cross-reactivity with antibodies against JEV, SLEV and DENV. The instant invention further provides a rapid, sensitive, and consistent method for the specific detection of WNV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-WNV antibodies but not cross-reactive with antibodies against other flaviviruses such as JEV, SLEV, or DENV. The present invention also provides a rapid, sensitive, and consistent method for the specific detection of DENV by employing diagnostic assays having the antigen NS5 which is specifically reactive with anti-DENV antibodies but do not cross-react with antibodies against other flaviviruses such as JEV, SLEV, or WNV. Further, the DENV NS5 antigens are serospecific and do not cross react with antibodies to other DENV strains. Thus, the method of the present invention provides a manner by which to discriminate infections by each DENV strain. Further, diagnostic kits for carrying out the methods are provided. The methods and kits for carrying out the methods of the invention are rapid and require as little as 10 minutes to detect a result.

An automatic detection method and a system for human body falling-over are based on a wireless network signal transmission technology. The method comprises the following steps: a wireless receiving terminal receives wireless signals from a wireless transmitting terminal and assesses channel state information; abnormity of the channel state information change is identified by using an abnormity detection algorithm; based on a one-class support vector machine which distinguishes a target motion kind from other motion kinds, an abnormity mode that a human body falling-over motion leads to a channel state information change is set as the target motion kind to determine whether a human body falling-over motion happens; if the human body falling-over motion happens, a falling-over alarm signal is emitted. The false alarm rate of the detection method provided by the invention is low; by utilizing a present wireless network and a device, other specific detection devices are not needed, and the detection method is high in universality; and at the same time people to be detected do not need to carry any extra sensing equipment, and inconvenience caused by that people to be detected carry detection equipment is avoided.

Apparatus and methods in which very small volumes of material may be extracted, delivered, interrogated or stimulated via optical, electromagnetic or mechanical means, in vivo or in vitro, for site-specific detection, characterization, stimulation, diagnostics or therapy, comprising optical, fluidic, chemical, electromagnetic and biological techniques applied via a microprobe in a single intra-parenchymal tissue perforation procedure in the brain. The primary use of the device is in neuroscience research, clinical diagnostics and therapeutics applications in the brain, however, the device may also be beneficially applied to other organs and biological systems. Human clinical applications may include neurosurgical intra-operative monitoring, extra-operative chronic monitoring of devices introduced in an operation, and diagnostic monitoring combined with simultaneous neuroimaging.

The present invention provides a novel class of molecules, termed “compomers,” that enable the indirect detection of target molecules, as well as novel target detection reagents and compomer templates that encode compomers. Compomers are linear polymers generated from the compomer template portion of a target detection reagent during the course of an assay. In a given assay, each compomer species is correlated with a different target molecule, e.g., a carbohydrate, lipid, polypeptide, or target nucleic acid, particularly a specific nucleotide sequence within a target nucleic acid molecule. When, for example, a target nucleic acid is present in an assay, a compomer species specifically and uniquely correlated with the particular target (e.g., a known SNP or other genetic variant) is generated directly from a target-specific detection reagent (or indirectly from a larger precursor encoded by the compomer template and from which it is subsequently released), after which it can readily be detected, even in an assay where tens, hundreds, or thousands of different compomer species may be generated, as each compomer species is engineered to differ from the others by a small, resolvable defined characteristic (e.g., a mass increment, a difference in subunit composition, sequence, size, length, etc.). When coupled with highly sensitive detection techniques (e.g., MALDI-TOF mass spectrometry, nucleic acid hybridization, nuclear magnetic resonance, etc.), a large number of different compomer species can be detected in a single reaction, thereby facilitating highly multiplexed analyses of complex samples.

Devices related to a near-drowning behavior detection method fusing UWB indoor location with a video target detection and tracking technology comprise a smart bracelet module, an intelligent monitoring information processing module, a communication module and a display alarm module, the smart bracelet module is used for performing indoor location, the intelligent monitoring information processingmodule is used for processing video data acquired by an intelligent monitoring camera, location information of a smart bracelet and water depth information; and the communication module is used for realizing information transfer among the smart bracelet module, the intelligent monitoring information processing module and the display alarm module. Specific detection steps of the near-drowning behavior detection method are performed according to the following way: performing system initialization; and building a relationship among an image of the intelligent monitoring camera, location of the smart bracelet module and a swimming pool coordinate system, then setting near-drowning conditions, and performing near-drowning determination by virtue of a near-drowning detection algorithm. The near-drowning behavior detection method is simple in main body structure, smart in design concept, good in using effect, high in detection accuracy, low in maintenance cost, friendly in application environment and broad in market prospect.

Method for specific detection of one or more analytes in a sample. The method includes specifically associating any one or more analytes in the sample with a scattered-light detectable particle, illuminating any particle associated with the analytes with light under conditions which produce scattered light from the particle and in which light scattered from one or more particles can be detected by a human eye with less than 500 times magnification and without electronic amplification. The method also includes detecting the light scattered by any such particles under those conditions as a measure of the presence of the analytes.

Described herein are novel SCCmec right extremity junction (MREJ) sequences for the detection and/or identification of methicillin-resistant Staphylococcus aureus (MRSA). Disclosed are methods and compositions based on DNA sequences for the specific detection of MREJ sequences designated types xi, xii, xiii, xiv, xv, xvi, xvii, xviii, xix, and xx for diagnostic purposes and/or epidemiological typing.

The invention discloses a fluorescent probe for detecting formaldehyde as well as a preparation method and an application of the fluorescent probe. The structure of the fluorescent probe is presented by a formula I (shown in the specification). A kit for detecting formaldehyde comprises a compound represented by the formula I and a solvent. The ''off-on'' type formaldehyde probe for naphthalimide dyes and the specific detection kit have good responses to a formaldehyde solution, can be used for detecting formaldehyde in cells and have the characteristics of being simple and convenient to operate, low in cost, sensitive in response and easy to popularize and apply.