123 results about "Molecular targets" patented technology

The present invention, among other things, provides technologies for detecting and / or quantifying nucleic acids in cells, tissues, organs, or organisms. Pre-designed barcodes are associated with specific molecular targets through sequential hybridization experiments. A pseudo-color based barcoding scheme is described that overcomes the limitations in the previous generation of the technology. The current method can be applied to both in vitro and in situ analysis.

The invention provides a lung adenocarcinoma prognostic marker and a model, constructs a lung adenocarcinoma prognostic model based on mRNA and lncRNA related to programmed cell death (PCD), and synthesizes bioinformatics analysis and experiments to explore the biological effect of the lncRNA model in lung adenocarcinoma. The prognosis model covers all PCD pathways and various molecules, has stable prognosis prediction accuracy, and can provide a potential molecular target for personalized treatment. According to the method disclosed by the invention, the PCD-related mRNA and lncRNA are integrated to construct a lung adenocarcinoma diagnosis model suitable for Chinese population, so that the identification capability of tumor patient classification is enhanced from multiple aspects, the accuracy and reliability of prognosis prediction are improved, powerful support can be provided for promoting personalized treatment strategies of lung adenocarcinoma patients, and the method is suitable for popularization and application.

The present disclosure provides methods for encoding and decoding signals from barcodes in a plurality of molecular targets from images obtained from imaging based spatial genomics (ISG) experiments. This disclosure sets forth methods, in addition to use of the same, and other solutions to problems in the relevant field.

The invention relates to the technical field of biology, in particular to a molecular marker for identifying Holstein cattle with high fecundity and application of the molecular marker. According to the invention, an RBX1 gene is analyzed in cattle 150K genotype chip data, and SNP loci related to first and last hybridization interval characters are found; the method comprises the following steps: establishing a 30x re-sequencing database of high and low reproduction populations for the first time, and autonomously identifying the SNP sites of the high-fecundity Holstein cattle; an important molecular target is provided for the creation of a new gene editing breeding material in the later period, the creation of an autonomous breeding chip in China and the breeding of cattle.

The invention relates to the technical field of biomarkers, in particular to a biomarker composition for diabetic foot ulcer and application of the biomarker composition. The invention provides a biomarker combination for diabetic foot ulcer. The biomarker combination comprises a DDIT4 gene and a PKM gene. According to the invention, by integrating transcriptome sequencing data and single cell sequencing data, biomarkers DDIT4 and PKM closely related to diabetic foot ulcer (DFU) are successfully screened out. The expression of the two genes in a disease group is remarkably up-regulated, and the two genes are stably expressed in different data sets, so that a reliable molecular target is provided for early diagnosis of DFU.

The invention relates to the technical field of molecular detection, discloses a KASP molecular marker related to a sheep backfat thickness character, a detection primer group and application of the KASP molecular marker and the detection primer group, and aims to provide a KASP molecular marker for detecting a sheep Chr15: g.12672188 site Tgt; the invention discloses a specific primer group for C variation, which consists of the following three primers: a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 1, and a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 2, the nucleotide sequence of the locus C allele typing primer VIC is as shown in SEQ ID NO: 2. The invention discovers and discloses Tgt of Chr15: g.12672188 site in a sheep genome for the first time; the C single nucleotide polymorphism (SNP) is obviously associated with the backfat thickness character. Association analysis of 118 Auodurau lake rams proves that the back fat thickness of CC and TT genotype individuals is obviously higher than that of TC genotype individuals. The discovery reveals that the two homozygous genotypes of the site are unique genetic patterns of dominant genotypes, and a direct and reliable molecular target is provided for genetic improvement of the sheep backfat thickness.

The present disclosure provides methods for encoding and decoding signals from barcodes in a plurality of molecular targets from images obtained from imaging based spatial genomics (ISG) experiments. This disclosure sets forth methods, in addition to use of the same, and other solutions to problems in the relevant field.

Methods of treating a subject for a dissociative disorder and methods of screening agents for the ability to modulate dissociative and associative states in a subject are provided. In particular, agents that alter rhythmic neural activity in the posteromedial cortex can be used to modulate dissociative and associative states in a subject.

The invention provides an application of a super enhancer inhibitor JQ-1 in preparation of peripheral artery disease related drugs, relates to the technical field of biomedicine, and discloses a core effect of the super enhancer inhibitor JQ-1 in peripheral artery disease ischemia repair. The traditional Chinese medicine composition can significantly accelerate blood flow recovery of ischemic limbs, up-regulate mRNA and protein expression of a vascular marker CD31 in gastrocnemius muscle tissues, effectively increase vascular density and promote angiogenesis. Aiming at the defects of the existing treatment means in the aspect of ischemic tissue angiogenesis, the application provides a brand new molecular targeted treatment scheme for high-risk groups such as patients with diabetes-related peripheral artery diseases, and can significantly reduce the risk of lower limb amputation and the incidence rate of related cardiovascular adverse events such as coronary heart disease and stroke; the technical blank of specific targeted therapy of peripheral artery diseases is filled, a solid scientific basis and a key direction are provided for research and development of novel drugs, and the specific targeted therapy method has extremely high clinical application value and wide research and development prospects.

The present invention generally relates to systems and methods for imaging or determining nucleic acids in cells or other samples. In some cases, the transcriptome of a cell may be determined. Certain embodiments are generally directed to determining nucleic acids and other targets in a sample at relatively high resolutions. For instance, nucleic acid probes may be applied to sample, and binding of the nucleic acid probes to a target may be amplified using primary and secondary amplifier nucleic acids. In some cases, there is a maximum number of amplifier nucleic acids that can be bound to a target, e.g., the binding is saturatable, and cannot grow indefinitely, even in the presence of abundant reagents. This may be advantageous, for example, for controlling the brightness of each binding event, controlling the size of the amplified regions (e.g., during imaging), and / or for limiting the degree of amplification noise (i.e. the final variation in amplified signal from molecule to molecule), etc. In addition, in some embodiments, the primary and / or secondary amplifier nucleic acids may be formed from only 3 of the 4 naturally-occurring nucleotides, which may result in less secondary structure, faster binding rates, etc. These properties can in some cases facilitate the rapid design of multiple orthogonal amplification sequences, allowing the extension of such an approach to many distinct molecular targets.

The invention provides methods for the detection of molecular targets by digital PCR (dPCR) using a set of universal probes and target-specific tailed primers. Each target is amplified by a unique mixture of primers. The tailed amplicons anneal to a universal set of probes to detect the associated targets. Some targets are amplified using more than one tailed primer. Some targets are amplified using the same multiple tailed primers. In these embodiments, the primers are concentrated to produce a different number of amplicons for each tailed primer, resulting in a different probe-amplicon balance for each target. Two colors of fluorescence intensity are read and plotted as a 2D plot. In the plot, different targets contribute well-resolved clusters. Each cluster in the plot essentially lies a long its own radius allowing for radial multiplexing. The use of a universal set of probes in multiple assays provides for greater flexibility and throughput.

The application discloses a set of molecular targets, primer compositions, methods and applications for identifying respiratory pathogens, the respiratory pathogens are novel coronavirus, influenza A virus and respiratory syncytial virus, and nucleotide sequences of the molecular targets are shown as SEQ ID NO:1-3. The application provides molecular targets, primers and probes for detecting three respiratory pathogens, and establishes a triple real-time RT-RAA detection technology, which can realize rapid identification and differentiation of three respiratory infectious disease pathogens with similar clinical symptoms within 30 minutes, reduces the operation threshold of the existing detection technology, realizes the target of rapidly, simply and accurately identifying and differentiating the three viruses, meets the needs of early clinical diagnosis of infection, provides technical support for precise medication, and has wide application value.

The application discloses a method for improving secretion of antibacterial peptide of Bombyx mori and application thereof, and first proposes application of genes as a regulation target or a regulation marker in improving secretion of antibacterial peptide Gloverin of Bombyx mori, and the application can effectively improve expression of antibacterial peptide Gloverin of Bombyx mori and prolong death time of Bombyx mori infected with nucleopolyhedrovirus by injecting RNAi interference fragments of 7 genes into first-day fifth-instar larvae of Bombyx mori after 6 hours, which provides a new thought for improving expression of antibacterial peptide by taking the like as a molecular target, is favorable for in-depth understanding of a mechanism of Bombyx mori against pathogenic agents, provides a reference for research on Bombyx mori against viruses, and provides a valuable reference for in-depth research on the role of 7 in immune response of Bombyx mori.

The invention relates to application of WDR74 and / or ALYREF as molecular targets in esophageal squamous cell carcinoma diagnosis and treatment, and belongs to the technical field of biological medicine. Aiming at the problem that the esophageal squamous cell carcinoma lacks an effective targeted treatment means, the invention discovers that the expression quantity of WDR74 in tumor tissues is obviously higher than that in para-carcinoma tissues, and the high expression of WDR74 prompts poor prognosis of a patient, and reveals that WDR74 protein and ALYREF protein have specific binding, and the mRNA stability of EGFR is enhanced through ALYREF-mediated m5C RNA epigenetic modification, so that STAT3 phosphorylation is activated, and the treatment effect of the esophageal squamous cell carcinoma is enhanced. Further, the STAT3 is combined with the promoter region of the apoptosis-inhibiting gene MCL1, and finally cell apoptosis is inhibited and tumor formation is promoted. The invention provides a new molecular target and a solution for developing a WDR74 and ALYREF targeting medicine for treating esophageal squamous cell carcinoma and related diagnosis and prognosis evaluation products.

The present disclosure provides methods for scalable amplification of an analyte signal in a sample to achieve spatial localization of the analyte in a biological sample. The present disclosure sets forth the methods, as well as the use of these methods and solutions to other issues in the related art.

The application discloses specific molecular targets of Vibrio alginolyticus and Vibrio fluvialis and a detection method thereof, wherein the nucleotide sequence of the specific molecular target of the Vibrio alginolyticus is as shown in SEQ ID NO. 1, and the nucleotide sequence of the specific molecular target of the Vibrio fluvialis is as shown in SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. The application not only discloses one specific molecular target for identifying the Vibrio alginolyticus and four specific molecular targets for identifying the Vibrio fluvialis, but also provides related specific primer sequences and corresponding PCR detection methods. The application has the advantages of short detection time, low cost, strong specificity and the like without physiological and biochemical identification.

The invention provides an application of an SLCO2B1-Ta transcript isomer as a hepatocellular carcinoma diagnosis and treatment target spot. The SLCO2B1-Ta transcript comprises a nucleotide sequence as shown in SEQ ID NO: 1. The invention not only provides a novel molecular target for diagnosis and treatment of hepatocellular carcinoma, but also lays an important experimental foundation for developing a targeted therapy scheme based on an RNA interference technology.

The invention provides an MaSBP17 gene and application thereof in regulating and controlling fruit maturity, and belongs to the technical field of gene engineering. The nucleotide sequence of the MaSBP17 gene is shown as SEQ ID NO: 1 in a sequence table. According to the invention, an SBP transcription factor family in a banana genome is comprehensively and systematically analyzed, a member MaSBP17 with significant expression change in a fruit ripening process is found, and the function of the MaSBP17 is verified by using a virus-induced gene silencing technology. The invention provides an important theoretical basis and a potential molecular target for targeted regulation and control of a banana fruit ripening process, delaying of softening and aging and development of a novel postharvest preservation strategy based on key transcription factors, and has remarkable scientific value and application prospect.

The invention relates to a biomarker used as a diabetic neurogenic bladder (DNB) and a screening method and application thereof. Specifically, the invention provides a biomarker for the neurogenic bladder of diabetes, and the biomarker comprises one or more of ICAM2, VEGFA, JAML, PTPRJ, PTPRC, CD40, IL2RB and NCR1. By utilizing the biomarker provided by the invention, non-invasive and efficient identification of DNB can be realized, and a new molecular target is provided for complication prevention and control of diabetic patients.

The invention discloses a TaqMan probe qPCR (quantitative polymerase chain reaction) detection primer group for detecting pathogenic vibrio alginolyticus, a kit and application, and belongs to the technical field of molecular biology and pathogenic microorganism detection. The method comprises the following steps: designing a pair of specific primers and probes by taking the vops gene of the vibrio alginolyticus as a new target, connecting the vops gene with plasmids by amplifying the vops gene, constructing a recombinant plasmid standard substance, carrying out qPCR detection on the copy number of the vops gene of the vibrio alginolyticus by utilizing the designed specific primers and probes, and establishing a corresponding relationship between the vibrio alginolyticus and the vops gene. And the vibrio alginolyticus can be quantified through the copy number of the vops gene. On the basis of novel molecular target detection, no amplification signal exists for a detection sample which does not contain vibrio alginolyticus, and the specificity is good; the method is rapid and accurate in detection and high in sensitivity, the lower detection limit is as low as 100-level copies, non-specific amplification can be avoided, and operation is simple and rapid.

The invention relates to an application of NUDT14 in improving the sensitivity of Enzalocamide to prostatic cancer treatment. Through deep research, it is clear that NUDT14 can be used as a key factor for occurrence and development of prostate cancer and generation of enzalocamide treatment drug resistance, activities such as proliferation and metastasis of prostate cancer can be obviously inhibited by inhibiting NUDT14 or reducing the biological activity of NUDT14, the drug resistance of enzalocamide is reversed, the treatment sensitivity of enzalocamide is improved, and the survival rate of patients is prolonged; the prognosis is improved. According to the invention, related mechanisms of drug resistance generation and regulation in the process of treating prostatic cancer by enzalocamide are enriched, sufficient scientific basis and theoretical basis are provided for searching new prostatic cancer diagnosis, prognosis judgment and treatment molecular targets and developing new targeted drugs, accurate treatment can be better realized, and the application of enzalocamide in treatment of prostatic cancer is promoted. The method has important social value and scientific significance.

The application belongs to the field of microbial molecule detection, and discloses a multiple qPCR kit and method for simultaneously detecting multiple listeria based on new molecular targets. The application designs corresponding primers and probes based on specific gene fragments of each listeria obtained by genomics mining, establishes a multiple qPCR method, and assembles the method into a portable kit. The multiple qPCR primer probe set of the application has high specificity, and only the six target bacteria of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, Listeria welshimeri, Listeria seeligeri and Listeria grayi appear fluorescence signals in the specific fluorescence signal channel, so that the detection result is more accurate. The multiple qPCR kit of the application can complete sample detection within 14 hours, wherein the whole qPCR process only needs about 1 hour, the detection efficiency is high, the detection cost is low, and the kit is particularly suitable for large-scale sample investigation and actual detection, and the detection sensitivity can be as low as 1.0-11.1 CFU / Test.

This invention discloses molecular markers for lung cancer diagnosis, chemotherapy, or prognosis detection, and their applications, relating to the field of molecular diagnostics. The molecular markers include trypsin 3 splice variant 3 and the transcription factor bone marrow zinc finger gene 1 splice variant 2. By detecting the expression levels of the MZF1-V2 and PRSS3-V3 genes, lung cancer diagnosis, chemotherapy, or prognosis can be achieved. The introduction of these molecular markers has significant scientific and clinical value in personalized precision treatment applications that improve chemotherapy sensitivity. It not only provides effective molecular targets for personalized precision treatment of lung cancer patients but also provides new evidence for novel intervention strategies. Furthermore, the introduction of these molecular markers provides a more reliable means of lung cancer diagnosis and prognosis prediction.

The invention discloses a preparation method and application of a pine resin canker pathogen protoplast, and belongs to the technical field of genetic transformation. According to the preparation method of the pine resin canker pathogen protoplast disclosed by the invention, the protoplast with the final concentration of 1-2 * 10 < 7 > / mL is obtained through operations of enzymolysis, filtration, centrifugal resuspension and the like. The invention also establishes a Fusarium circinatum NAUA-1 genetic transformation system, and the genetic transformation system comprises the steps of protoplast preparation, plasmid transformation and screening, so as to obtain a stable genetic transformation strain FcGFP-1. Results of the embodiment of the invention show that the protoplast preparation method is efficient, stable and simple and convenient to operate, a transformation system can realize stable integration and expression of an exogenous gene, and the biological phenotype and pathogenicity of a transformed strain are consistent with those of a wild type; and technical support is provided for research on gene functions and pathogenic mechanisms of the pine canker germs, and drug molecular target mining and disease prevention and control are facilitated.

This invention discloses the application of the CEMIP-ITGA5 axis as a target in the preparation of products that regulate epithelial-mesenchymal transition (EMT) and metastasis in lung adenocarcinoma, belonging to the fields of tumor molecular biology and precision medicine. Through TCGA-LUAD cohort bioinformatics analysis, immunohistochemical verification of clinical specimens, cell function experiments, and a nude mouse subcutaneous xenograft model, this invention discovers and confirms that the CEMIP-ITGA5 axis participates in regulating epithelial-mesenchymal transition, migration, invasion, and metastasis-related malignant progression in lung adenocarcinoma. Specifically, CEMIP positively regulates ITGA5 expression, thereby promoting EMT, proliferation, migration, invasion, and in vivo tumorigenesis in lung adenocarcinoma cells. Based on this, the present invention provides two applications: Firstly, by detecting the expression levels of the dual genes CEMIP and ITGA5, a kit for assessing the risk of epithelial-mesenchymal transition (EMT) and metastasis in lung adenocarcinoma can be prepared. The combined detection of CEMIP and ITGA5 can reflect the activation status of the CEMIP-ITGA5 axis at both the upstream regulatory molecule and downstream effector molecule levels, providing auxiliary molecular evidence for assessing EMT and metastasis-related risks in lung adenocarcinoma. Secondly, by targeting and inhibiting the expression or function of CEMIP and / or ITGA5, drugs can be prepared to inhibit epithelial-mesenchymal transition, migration, invasion, and metastasis-related malignant progression in lung adenocarcinoma, effectively reversing the epithelial-mesenchymal transition phenotype and inhibiting tumor malignant progression. This invention provides novel molecular targets and technical solutions independent of known signaling pathways for prognostic assessment and targeted therapy of lung adenocarcinoma.

The invention relates to the technical field of molecular detection, and particularly discloses a molecular target for detecting colletotrichum ginseng. Aiming at the technical problems of long time consumption, poor specificity, difficulty in realizing accurate quantification of pathogenic bacteria in soil and the like in the existing colletotrichum ginseng detection method, the invention provides a section of specific long nucleotide sequence of colletotrichum ginseng as a molecular target, and the nucleotide sequence is shown as SEQ ID NO: 1. A specific primer group designed based on the molecular target, a kit containing the primer group and a non-diagnostic purpose detection method jointly form a complete detection system, and rapid, sensitive and specific qualitative and accurate quantitative detection of the colletotrichum ginseng in ginseng plant tissues, seeds and soil samples can be realized; and a reliable technical tool is provided for early warning of ginseng anthracnose, dynamic monitoring of pathogenic bacteria and scientific prevention and control.

The invention relates to application of HNRNPA3 in treatment and prognosis evaluation of prostate cancer. Through deep research, the HNRNPA3 is determined to be a key target spot for prognosis judgment and treatment of the neuroendocrine prostate cancer, can be used for treatment, auxiliary diagnosis and prognosis evaluation of the neuroendocrine prostate cancer, and meanwhile, improves the understanding of the pathogenesis of the neuroendocrine prostate cancer to a new height; the neuroendocrine prostate cancer pathogenesis content is greatly enriched, a sufficient scientific basis and theoretical basis are provided for searching new prostate cancer diagnosis, prognosis judgment and treatment molecular targets and developing new targeted drugs, and important social value and scientific significance are achieved.

An ornithine decarboxylase gene related to Auricularia auricula-judae degradation and application thereof belong to the field of bioengineering technology. In order to solve the technical problem that there is lack of key functional genes and molecular targets which can be used for early identification, anti-degradation molecular breeding and strain rejuvenation due to continuous subculture leading to degradation in Auricularia auricula-judae industry, based on the G1, G10 and G20 mycelium transcriptome difference analysis of Auricularia auricula-judae strain Heiwei S1908, the ornithine decarboxylase gene g4162 significantly differentially expressed between normal mycelium and G20 degraded mycelium is screened, the molecular function of the gene in the degradation of Auricularia auricula-judae subculture is determined, and the system biology information prediction of the gene coding protein is completed, which provides core gene resources and theoretical basis for the degradation mechanism research, molecular detection kit development, anti-degradation germplasm screening and gene breeding of Auricularia auricula-judae.

The invention provides a detection reagent for LXOL2 gene expression quantity and application of LXOL2 as a rheumatoid arthritis treatment target, and relates to the technical field of biological medicines. According to an extracted total RNA sequence, a specific PCR primer is designed and synthesized, an RT-qPCR technology and a sequencing method are utilized, the expression of the LXOL2 in human synovial fibroblast-like cells is found to be remarkably increased, it is proved that the LXOL2 can be applied to preparation of a preparation for auxiliary diagnosis, treatment or prognosis evaluation of rheumatoid arthritis, meanwhile, Si-LOXL2 small in interference with the gene is designed and synthesized, and the application prospect is wide. According to the present invention, the expression of the LXOL2 gene can be knocked down, the expression of pro-inflammatory factors TNF-alpha, IL-1beta and IL-6 can be significantly inhibited, the expression of anti-inflammatory factors TGF-beta can be promoted, and the gene can be used as the RA molecular targeting treatment tool;

This invention discloses the use of Chek2 inhibitors in the preparation of drugs for the prevention and / or treatment of postoperative pain sensitization induced or aggravated by chronic sleep disorders, and its intervention methods, relating to the field of biomedical technology. This invention provides the use of Chek2 inhibitors in the preparation of drugs for the prevention and / or treatment of postoperative pain sensitization induced or aggravated by chronic sleep disorders. This invention is the first to discover and validate Chek2 as a key molecular target linking "chronic sleep disorders" and "postoperative pain sensitization." By constructing a composite animal model of chronic sleep deprivation (CSD) combined with postoperative incision pain, the pathological process of increased postoperative pain in patients with sleep disorders was simulated. Using mRNA-seq and Western blot techniques, this invention is the first to discover and confirm that the expression level of Chek2 in the nucleus accumbens (NAc) brain region is significantly upregulated during postoperative pain sensitization induced by chronic sleep disorders, filling a gap in the field of "sleep disorder-pain interaction" regarding specific central targets.