83 results about "Molecular targets" patented technology

The invention relates to the technical field of biology, in particular to a molecular marker for identifying Holstein cattle with high fecundity and application of the molecular marker. According to the invention, an RBX1 gene is analyzed in cattle 150K genotype chip data, and SNP loci related to first and last hybridization interval characters are found; the method comprises the following steps: establishing a 30x re-sequencing database of high and low reproduction populations for the first time, and autonomously identifying the SNP sites of the high-fecundity Holstein cattle; an important molecular target is provided for the creation of a new gene editing breeding material in the later period, the creation of an autonomous breeding chip in China and the breeding of cattle.

The invention relates to the technical field of molecular detection, discloses a KASP molecular marker related to a sheep backfat thickness character, a detection primer group and application of the KASP molecular marker and the detection primer group, and aims to provide a KASP molecular marker for detecting a sheep Chr15: g.12672188 site Tgt; the invention discloses a specific primer group for C variation, which consists of the following three primers: a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 1, and a site T allele typing primer FAM with a nucleotide sequence as shown in SEQ ID NO: 2, the nucleotide sequence of the locus C allele typing primer VIC is as shown in SEQ ID NO: 2. The invention discovers and discloses Tgt of Chr15: g.12672188 site in a sheep genome for the first time; the C single nucleotide polymorphism (SNP) is obviously associated with the backfat thickness character. Association analysis of 118 Auodurau lake rams proves that the back fat thickness of CC and TT genotype individuals is obviously higher than that of TC genotype individuals. The discovery reveals that the two homozygous genotypes of the site are unique genetic patterns of dominant genotypes, and a direct and reliable molecular target is provided for genetic improvement of the sheep backfat thickness.

Methods of treating a subject for a dissociative disorder and methods of screening agents for the ability to modulate dissociative and associative states in a subject are provided. In particular, agents that alter rhythmic neural activity in the posteromedial cortex can be used to modulate dissociative and associative states in a subject.

The invention provides an application of a super enhancer inhibitor JQ-1 in preparation of peripheral artery disease related drugs, relates to the technical field of biomedicine, and discloses a core effect of the super enhancer inhibitor JQ-1 in peripheral artery disease ischemia repair. The traditional Chinese medicine composition can significantly accelerate blood flow recovery of ischemic limbs, up-regulate mRNA and protein expression of a vascular marker CD31 in gastrocnemius muscle tissues, effectively increase vascular density and promote angiogenesis. Aiming at the defects of the existing treatment means in the aspect of ischemic tissue angiogenesis, the application provides a brand new molecular targeted treatment scheme for high-risk groups such as patients with diabetes-related peripheral artery diseases, and can significantly reduce the risk of lower limb amputation and the incidence rate of related cardiovascular adverse events such as coronary heart disease and stroke; the technical blank of specific targeted therapy of peripheral artery diseases is filled, a solid scientific basis and a key direction are provided for research and development of novel drugs, and the specific targeted therapy method has extremely high clinical application value and wide research and development prospects.

The application discloses a set of molecular targets, primer compositions, methods and applications for identifying respiratory pathogens, the respiratory pathogens are novel coronavirus, influenza A virus and respiratory syncytial virus, and nucleotide sequences of the molecular targets are shown as SEQ ID NO:1-3. The application provides molecular targets, primers and probes for detecting three respiratory pathogens, and establishes a triple real-time RT-RAA detection technology, which can realize rapid identification and differentiation of three respiratory infectious disease pathogens with similar clinical symptoms within 30 minutes, reduces the operation threshold of the existing detection technology, realizes the target of rapidly, simply and accurately identifying and differentiating the three viruses, meets the needs of early clinical diagnosis of infection, provides technical support for precise medication, and has wide application value.

The application discloses a method for improving secretion of antibacterial peptide of Bombyx mori and application thereof, and first proposes application of genes as a regulation target or a regulation marker in improving secretion of antibacterial peptide Gloverin of Bombyx mori, and the application can effectively improve expression of antibacterial peptide Gloverin of Bombyx mori and prolong death time of Bombyx mori infected with nucleopolyhedrovirus by injecting RNAi interference fragments of 7 genes into first-day fifth-instar larvae of Bombyx mori after 6 hours, which provides a new thought for improving expression of antibacterial peptide by taking the like as a molecular target, is favorable for in-depth understanding of a mechanism of Bombyx mori against pathogenic agents, provides a reference for research on Bombyx mori against viruses, and provides a valuable reference for in-depth research on the role of 7 in immune response of Bombyx mori.

The invention relates to application of WDR74 and / or ALYREF as molecular targets in esophageal squamous cell carcinoma diagnosis and treatment, and belongs to the technical field of biological medicine. Aiming at the problem that the esophageal squamous cell carcinoma lacks an effective targeted treatment means, the invention discovers that the expression quantity of WDR74 in tumor tissues is obviously higher than that in para-carcinoma tissues, and the high expression of WDR74 prompts poor prognosis of a patient, and reveals that WDR74 protein and ALYREF protein have specific binding, and the mRNA stability of EGFR is enhanced through ALYREF-mediated m5C RNA epigenetic modification, so that STAT3 phosphorylation is activated, and the treatment effect of the esophageal squamous cell carcinoma is enhanced. Further, the STAT3 is combined with the promoter region of the apoptosis-inhibiting gene MCL1, and finally cell apoptosis is inhibited and tumor formation is promoted. The invention provides a new molecular target and a solution for developing a WDR74 and ALYREF targeting medicine for treating esophageal squamous cell carcinoma and related diagnosis and prognosis evaluation products.

The present disclosure provides methods for scalable amplification of an analyte signal in a sample to achieve spatial localization of the analyte in a biological sample. The present disclosure sets forth the methods, as well as the use of these methods and solutions to other issues in the related art.

The application discloses specific molecular targets of Vibrio alginolyticus and Vibrio fluvialis and a detection method thereof, wherein the nucleotide sequence of the specific molecular target of the Vibrio alginolyticus is as shown in SEQ ID NO. 1, and the nucleotide sequence of the specific molecular target of the Vibrio fluvialis is as shown in SEQ ID NO. 2, SEQ ID NO. 3, SEQ ID NO. 4 or SEQ ID NO. 5. The application not only discloses one specific molecular target for identifying the Vibrio alginolyticus and four specific molecular targets for identifying the Vibrio fluvialis, but also provides related specific primer sequences and corresponding PCR detection methods. The application has the advantages of short detection time, low cost, strong specificity and the like without physiological and biochemical identification.

The invention provides an application of an SLCO2B1-Ta transcript isomer as a hepatocellular carcinoma diagnosis and treatment target spot. The SLCO2B1-Ta transcript comprises a nucleotide sequence as shown in SEQ ID NO: 1. The invention not only provides a novel molecular target for diagnosis and treatment of hepatocellular carcinoma, but also lays an important experimental foundation for developing a targeted therapy scheme based on an RNA interference technology.

The invention provides an MaSBP17 gene and application thereof in regulating and controlling fruit maturity, and belongs to the technical field of gene engineering. The nucleotide sequence of the MaSBP17 gene is shown as SEQ ID NO: 1 in a sequence table. According to the invention, an SBP transcription factor family in a banana genome is comprehensively and systematically analyzed, a member MaSBP17 with significant expression change in a fruit ripening process is found, and the function of the MaSBP17 is verified by using a virus-induced gene silencing technology. The invention provides an important theoretical basis and a potential molecular target for targeted regulation and control of a banana fruit ripening process, delaying of softening and aging and development of a novel postharvest preservation strategy based on key transcription factors, and has remarkable scientific value and application prospect.

The invention discloses a TaqMan probe qPCR (quantitative polymerase chain reaction) detection primer group for detecting pathogenic vibrio alginolyticus, a kit and application, and belongs to the technical field of molecular biology and pathogenic microorganism detection. The method comprises the following steps: designing a pair of specific primers and probes by taking the vops gene of the vibrio alginolyticus as a new target, connecting the vops gene with plasmids by amplifying the vops gene, constructing a recombinant plasmid standard substance, carrying out qPCR detection on the copy number of the vops gene of the vibrio alginolyticus by utilizing the designed specific primers and probes, and establishing a corresponding relationship between the vibrio alginolyticus and the vops gene. And the vibrio alginolyticus can be quantified through the copy number of the vops gene. On the basis of novel molecular target detection, no amplification signal exists for a detection sample which does not contain vibrio alginolyticus, and the specificity is good; the method is rapid and accurate in detection and high in sensitivity, the lower detection limit is as low as 100-level copies, non-specific amplification can be avoided, and operation is simple and rapid.

The invention relates to an application of NUDT14 in improving the sensitivity of Enzalocamide to prostatic cancer treatment. Through deep research, it is clear that NUDT14 can be used as a key factor for occurrence and development of prostate cancer and generation of enzalocamide treatment drug resistance, activities such as proliferation and metastasis of prostate cancer can be obviously inhibited by inhibiting NUDT14 or reducing the biological activity of NUDT14, the drug resistance of enzalocamide is reversed, the treatment sensitivity of enzalocamide is improved, and the survival rate of patients is prolonged; the prognosis is improved. According to the invention, related mechanisms of drug resistance generation and regulation in the process of treating prostatic cancer by enzalocamide are enriched, sufficient scientific basis and theoretical basis are provided for searching new prostatic cancer diagnosis, prognosis judgment and treatment molecular targets and developing new targeted drugs, accurate treatment can be better realized, and the application of enzalocamide in treatment of prostatic cancer is promoted. The method has important social value and scientific significance.

The application belongs to the field of microbial molecule detection, and discloses a multiple qPCR kit and method for simultaneously detecting multiple listeria based on new molecular targets. The application designs corresponding primers and probes based on specific gene fragments of each listeria obtained by genomics mining, establishes a multiple qPCR method, and assembles the method into a portable kit. The multiple qPCR primer probe set of the application has high specificity, and only the six target bacteria of Listeria monocytogenes, Listeria innocua, Listeria ivanovii, Listeria welshimeri, Listeria seeligeri and Listeria grayi appear fluorescence signals in the specific fluorescence signal channel, so that the detection result is more accurate. The multiple qPCR kit of the application can complete sample detection within 14 hours, wherein the whole qPCR process only needs about 1 hour, the detection efficiency is high, the detection cost is low, and the kit is particularly suitable for large-scale sample investigation and actual detection, and the detection sensitivity can be as low as 1.0-11.1 CFU / Test.

This invention discloses molecular markers for lung cancer diagnosis, chemotherapy, or prognosis detection, and their applications, relating to the field of molecular diagnostics. The molecular markers include trypsin 3 splice variant 3 and the transcription factor bone marrow zinc finger gene 1 splice variant 2. By detecting the expression levels of the MZF1-V2 and PRSS3-V3 genes, lung cancer diagnosis, chemotherapy, or prognosis can be achieved. The introduction of these molecular markers has significant scientific and clinical value in personalized precision treatment applications that improve chemotherapy sensitivity. It not only provides effective molecular targets for personalized precision treatment of lung cancer patients but also provides new evidence for novel intervention strategies. Furthermore, the introduction of these molecular markers provides a more reliable means of lung cancer diagnosis and prognosis prediction.

The invention discloses a preparation method and application of a pine resin canker pathogen protoplast, and belongs to the technical field of genetic transformation. According to the preparation method of the pine resin canker pathogen protoplast disclosed by the invention, the protoplast with the final concentration of 1-2 * 10 < 7 > / mL is obtained through operations of enzymolysis, filtration, centrifugal resuspension and the like. The invention also establishes a Fusarium circinatum NAUA-1 genetic transformation system, and the genetic transformation system comprises the steps of protoplast preparation, plasmid transformation and screening, so as to obtain a stable genetic transformation strain FcGFP-1. Results of the embodiment of the invention show that the protoplast preparation method is efficient, stable and simple and convenient to operate, a transformation system can realize stable integration and expression of an exogenous gene, and the biological phenotype and pathogenicity of a transformed strain are consistent with those of a wild type; and technical support is provided for research on gene functions and pathogenic mechanisms of the pine canker germs, and drug molecular target mining and disease prevention and control are facilitated.

This invention discloses the application of the CEMIP-ITGA5 axis as a target in the preparation of products that regulate epithelial-mesenchymal transition (EMT) and metastasis in lung adenocarcinoma, belonging to the fields of tumor molecular biology and precision medicine. Through TCGA-LUAD cohort bioinformatics analysis, immunohistochemical verification of clinical specimens, cell function experiments, and a nude mouse subcutaneous xenograft model, this invention discovers and confirms that the CEMIP-ITGA5 axis participates in regulating epithelial-mesenchymal transition, migration, invasion, and metastasis-related malignant progression in lung adenocarcinoma. Specifically, CEMIP positively regulates ITGA5 expression, thereby promoting EMT, proliferation, migration, invasion, and in vivo tumorigenesis in lung adenocarcinoma cells. Based on this, the present invention provides two applications: Firstly, by detecting the expression levels of the dual genes CEMIP and ITGA5, a kit for assessing the risk of epithelial-mesenchymal transition (EMT) and metastasis in lung adenocarcinoma can be prepared. The combined detection of CEMIP and ITGA5 can reflect the activation status of the CEMIP-ITGA5 axis at both the upstream regulatory molecule and downstream effector molecule levels, providing auxiliary molecular evidence for assessing EMT and metastasis-related risks in lung adenocarcinoma. Secondly, by targeting and inhibiting the expression or function of CEMIP and / or ITGA5, drugs can be prepared to inhibit epithelial-mesenchymal transition, migration, invasion, and metastasis-related malignant progression in lung adenocarcinoma, effectively reversing the epithelial-mesenchymal transition phenotype and inhibiting tumor malignant progression. This invention provides novel molecular targets and technical solutions independent of known signaling pathways for prognostic assessment and targeted therapy of lung adenocarcinoma.

The invention relates to the technical field of molecular detection, and particularly discloses a molecular target for detecting colletotrichum ginseng. Aiming at the technical problems of long time consumption, poor specificity, difficulty in realizing accurate quantification of pathogenic bacteria in soil and the like in the existing colletotrichum ginseng detection method, the invention provides a section of specific long nucleotide sequence of colletotrichum ginseng as a molecular target, and the nucleotide sequence is shown as SEQ ID NO: 1. A specific primer group designed based on the molecular target, a kit containing the primer group and a non-diagnostic purpose detection method jointly form a complete detection system, and rapid, sensitive and specific qualitative and accurate quantitative detection of the colletotrichum ginseng in ginseng plant tissues, seeds and soil samples can be realized; and a reliable technical tool is provided for early warning of ginseng anthracnose, dynamic monitoring of pathogenic bacteria and scientific prevention and control.

An ornithine decarboxylase gene related to Auricularia auricula-judae degradation and application thereof belong to the field of bioengineering technology. In order to solve the technical problem that there is lack of key functional genes and molecular targets which can be used for early identification, anti-degradation molecular breeding and strain rejuvenation due to continuous subculture leading to degradation in Auricularia auricula-judae industry, based on the G1, G10 and G20 mycelium transcriptome difference analysis of Auricularia auricula-judae strain Heiwei S1908, the ornithine decarboxylase gene g4162 significantly differentially expressed between normal mycelium and G20 degraded mycelium is screened, the molecular function of the gene in the degradation of Auricularia auricula-judae subculture is determined, and the system biology information prediction of the gene coding protein is completed, which provides core gene resources and theoretical basis for the degradation mechanism research, molecular detection kit development, anti-degradation germplasm screening and gene breeding of Auricularia auricula-judae.

The invention provides a detection reagent for LXOL2 gene expression quantity and application of LXOL2 as a rheumatoid arthritis treatment target, and relates to the technical field of biological medicines. According to an extracted total RNA sequence, a specific PCR primer is designed and synthesized, an RT-qPCR technology and a sequencing method are utilized, the expression of the LXOL2 in human synovial fibroblast-like cells is found to be remarkably increased, it is proved that the LXOL2 can be applied to preparation of a preparation for auxiliary diagnosis, treatment or prognosis evaluation of rheumatoid arthritis, meanwhile, Si-LOXL2 small in interference with the gene is designed and synthesized, and the application prospect is wide. According to the present invention, the expression of the LXOL2 gene can be knocked down, the expression of pro-inflammatory factors TNF-alpha, IL-1beta and IL-6 can be significantly inhibited, the expression of anti-inflammatory factors TGF-beta can be promoted, and the gene can be used as the RA molecular targeting treatment tool;

This invention discloses the use of Chek2 inhibitors in the preparation of drugs for the prevention and / or treatment of postoperative pain sensitization induced or aggravated by chronic sleep disorders, and its intervention methods, relating to the field of biomedical technology. This invention provides the use of Chek2 inhibitors in the preparation of drugs for the prevention and / or treatment of postoperative pain sensitization induced or aggravated by chronic sleep disorders. This invention is the first to discover and validate Chek2 as a key molecular target linking "chronic sleep disorders" and "postoperative pain sensitization." By constructing a composite animal model of chronic sleep deprivation (CSD) combined with postoperative incision pain, the pathological process of increased postoperative pain in patients with sleep disorders was simulated. Using mRNA-seq and Western blot techniques, this invention is the first to discover and confirm that the expression level of Chek2 in the nucleus accumbens (NAc) brain region is significantly upregulated during postoperative pain sensitization induced by chronic sleep disorders, filling a gap in the field of "sleep disorder-pain interaction" regarding specific central targets.

A computer-implemented method may collect a set of known drug compounds from a database and create an enhanced dataset using labeled and unlabeled chemical and biological data. The computer-implemented method may train a neural network using the extended training set to generate a predicted score of the biological activity of the candidate drug compound to the biological target, where the contribution of a second plurality of unlabeled samples in training the neural network is scaled by a parameter. The computer-implemented method may output one or more fine tuning neural network models capable of generating candidate drug compounds with predicted activity. A computer-implemented method may generate a candidate drug compound by inputting the candidate drug compound having chemical data into a trained neural network model.

The invention relates to an effect of limited uptake of arachidonic acid (AA) in preparation of a chemotherapy sensitizer, and particularly relates to an application of the arachidonic acid (AA) in preparation of the chemotherapy sensitizer. A large number of researches find that the AA content is extremely related to the colorectal cancer chemotherapy resistance, the high level of AA has negative correlation with the colorectal cancer chemotherapy sensitivity, the AA can be used for predicting the colorectal cancer chemotherapy tolerance and sensitivity degree, and early evaluation is provided for the relation of preoperative treatment of a patient. Meanwhile, AA uptake is limited, for example, AA transporter SLC27A4 is inhibited, an important function of colorectal cancer chemotherapy sensitization is achieved, correspondingly, the tolerance of a patient to colorectal cancer chemotherapy can be reduced by reducing the level of AA in a body, the chemotherapy treatment effect is improved, the survival rate of the patient is prolonged, and prognosis is improved. The invention provides sufficient scientific basis and theoretical basis for exploring new colorectal cancer diagnosis, prognosis judgment and treatment molecular targets and developing new targeted drugs, is helpful for better realizing accurate treatment, and has important social value and scientific significance.

The present application relates to the technical field of targeted drug matching, and particularly relates to a qi deficiency and dampness accumulation type constipation targeted drug matching method and system and a storage medium, which comprises the following steps: collecting patient related characteristic data, calculating the activation degree score of each traditional Chinese medicine target point of the patient based on a preset three-level traditional Chinese medicine target point system, and determining the qi deficiency weight and dampness accumulation weight of the patient; according to the activation degree score, mapping the priority weight of the corresponding molecular target point through the preset mapping relationship between the traditional Chinese medicine target point and the molecular target point bidirectional binding, obtaining the double target point priority matrix of the patient; based on the double target point priority matrix, setting the weighting coefficient of each target point with the qi deficiency weight and the dampness accumulation weight, calculating the double target point matching degree of the simultaneous matching of the traditional Chinese medicine target point and the molecular target point in the drugs and drug combinations of the qi deficiency and dampness accumulation type constipation prescription, and generating a drug use scheme. The present application realizes the synergistic matching of the traditional Chinese medicine and western medicine double target points of the qi deficiency and dampness accumulation type constipation, improves the drug precision and clinical curative effect, and reduces the side effects and recurrence rate.

The system disclosed comprises an apparatus and a method for the detection and quantification of targeted molecules in vivo. The apparatus comprises an implantable biosensor and an AC magnetic detection device. The implantable biosensor includes functionalized nanoparticles functionalized with one or more moieties that bind to a molecular target of interest. The nanoparticles are retained in a biocompatible container which allows the molecular target of interest to enter the biosensor, for example through a semipermeable port. The biosensor can be implanted minimally-invasively into humans or animals. Upon exposure of the nanoparticles to the molecular target, a change in Neel relaxation time can be externally detected and correlated to the target analytes concentration. The change in relaxation time is detected through magnetic AC spectrometric measurements at a frequency specifically tuned to the nanoparticle type of interest Additionally, a method is provided for quantifying one or several biosensors within one specimen.

The application relates to the technical field of gene targeting regulation, and provides application of siRNA targeting RNF24 in preparation of a liver cancer treatment drug. The sense strand and the antisense strand of the siRNA targeting RNF24 have nucleotide sequences shown in SEQ ID NO. 1-SEQ ID NO. 10, the siRNA can treat liver cancer by inhibiting the expression of an RNF24 gene and reducing the synthesis of a protein coded by the RNF24 gene, and can reduce the proliferation and migration abilities of liver cancer cells. The application solves the problems that existing liver cancer molecular targeted treatment drugs are limited, sequence silencing efficiency and specificity are difficult to be considered in siRNA targeted treatment, stability is insufficient, and delivery efficiency is low.

The invention discloses a research method for promoting glioblastoma drug resistance by regulating YAP through P4HA1, and relates to the technical field of biology. The invention aims at researching whether P4HA1 has hydroxylation on YAP, so that the drug resistance of GBM to TMZ is influenced, and finally the proliferation condition of GBM is influenced, further exploring whether the treatment resistance of GBM can be improved after a P4HA1 inhibitor and a YAP inhibitor are combined with TMZ, and evaluating the possibility that P4HA1 and YAP are used as potential treatment targets by understanding the interaction between P4HA1 and YAP. According to the invention, a new treatment method capable of overcoming the drug resistance of GBM chemotherapy is found, the chemotherapy effect is improved, so that the prognosis of a patient is improved, the influence of P4HA1 and YAP on the drug sensitivity of GBM is innovatively evaluated, a new treatment strategy is provided for overcoming the clinical drug resistance problem, and potential signal channels and molecular targets are found.

The invention discloses a biomarker for diagnosing Alzheimer's disease as well as a screening method and application thereof, relates to the technical field of biomarkers for Alzheimer's disease, and aims to solve the technical problems that the Alzheimer's disease lacks dynamic pathological monitoring indexes and stereoisomeric modification is difficult to systematically detect. According to the invention, the stereoisomeric modification level of peptide fragments KLDLSNVQSK and AKTDHGAEIVYK is used as the core biomarker for the first time, the modification level is remarkably related to the Braak staging of AD, and a detection method and a combined diagnosis model containing the marker are provided. A novel molecular target with high correlation and dynamic monitoring potential is provided for AD, the model diagnosis precision is remarkably improved, and a brand new solution is provided for disease mechanism research and drug development.

The invention relates to a method for screening Alzheimer's disease markers by using a bioinformatics technology and application of the Alzheimer's disease markers. Serum microRNA samples of Alzheimer's disease normal subjects (NC), mild cognitive impairment (MCI) and Alzheimer's disease (AD) patients in an NCBI public database are analyzed, and a bioinformatics analysis method is adopted to systematically identify common miRNA which commonly presents differential expression at the early stage and the late stage of the disease, so that target mRNA of the common miRNA is predicted, and a regulatory network is constructed; and finally screening out specific miRNA closely associated with the key mRNA as a disease monitoring marker. The method breaks through the high-cost limitation of traditional dependence on high-throughput sequencing, realizes efficient and reliable screening and identification of disease molecular markers by deeply mining public data resources, provides a new molecular target and detection means for early diagnosis, illness monitoring and intervention treatment of Alzheimer's disease, and has a wide application prospect. The important clinical application value is realized.

The application discloses functions and application of KIF5B gene in regulating physiological activity of myoblasts, and belongs to the technical field of animal cell culture. By investigating influences of KIF5B knockdown and overexpression on proliferation of myoblasts, it is found that KIF5B knockdown can significantly inhibit DNA synthesis speed of myoblasts, thereby inhibiting proliferation. In addition, overexpression of KIF5B can significantly inhibit expression of MYOD and MYOG, improve DNA synthesis speed of myoblasts, thereby significantly improving proliferation rate of myoblasts. The application provides an important key molecular target and technical support for efficient and rapid culture of myogenic cells.