Mangosteen total xanthone extract and application thereof in preparation of TR3 receptor inducer

A technology of xanthone and extract is applied in the field of mangosteen total xanthone extract and preparation thereof, and the field of preparation of TR3 receptor inducer, which can solve the problems of unknown active substance and low technical content.

Active Publication Date: 2010-12-08
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above inventions are all mangosteen extracts obtained through simple ethanol extraction and purification, with low technical content and unknown active substances

Method used

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  • Mangosteen total xanthone extract and application thereof in preparation of TR3 receptor inducer
  • Mangosteen total xanthone extract and application thereof in preparation of TR3 receptor inducer
  • Mangosteen total xanthone extract and application thereof in preparation of TR3 receptor inducer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Preparation of the total xanthone fraction of mangosteen

[0029] Mangosteen peel 3kg, after crushing, add 90% (v / v) ethanol-water 25L to heat and reflux to extract twice, each time for 2 hours, after the combined extracts are filtered through gauze, centrifuged at 1000rpm for 5 minutes, take the supernatant The solution was concentrated, freeze-dried into a dry extract to obtain the mangosteen extract GM (360g). Take 290g of mangosteen extract, dissolve it in an appropriate amount of water, filter, and take the filtrate and subject it to macroporous resin HP-20 open column chromatography, ethanol-water gradient elution. Respectively recover under reduced pressure to obtain water elution fraction (109.0g), 30% (v / v) ethanol-water elution fraction (69.0g), 50% (v / v) ethanol-water elution fraction (16.0g) , and a 90% (v / v) ethanol-water elution fraction (88.0 g). Wherein, the 90% (v / v) ethanol-water eluted part is the GMD of the total xanthone part of mangost...

Embodiment 2

[0030] Example 2: Establishment of HPLC characteristic spectrum of total xanthone parts of mangosteen

[0031] The total xanthone parts of mangosteen obtained in Example 1 were dissolved with an appropriate amount of methanol to make a 5.0 mg / mL solution, and after passing through a 0.45 μm filter membrane, the test solution of the total xanthone parts of mangosteen was obtained.

[0032] Precisely take 10 μ L of the above-mentioned test product, refer to the high-performance liquid phase analysis method specified in the appendix of the National Pharmacopoeia, inject high-performance liquid chromatography for chromatographic analysis, and record the chromatogram ( figure 1 ). The liquid chromatography conditions are as follows: use octadecylsiloxane bonded silica gel as the stationary phase; use methanol-water solution containing 0.1% formic acid as the mobile phase, and gradient elution; the flow rate is 1mL / min; the detection wavelength is 360nm; the chromatographic column ...

Embodiment 3

[0036] Example 3: Investigation on the reproducibility of the HPLC characteristic spectrum of the total xanthone part of mangosteen

[0037] Get four batches of mangosteens purchased from different times (2007-05-05, 2008-05-15) and different origins (Guangdong, Thailand), and process them sequentially according to the preparation method in Example 1 to obtain four batches of total xanthenes of mangosteen Ketone parts (GD2007, TG2007, GD2008, TG2008), according to the processing method and liquid phase conditions in Example 2, carry out HPLC analysis respectively, obtain the HPLC characteristic collection of four batches of total xanthone parts of mangosteen, in which α-inverse The relative retention time of each chromatographic peak and the corresponding chemical substances of each chromatographic peak calculated by the chromatographic retention time of mangostin as 1 are as follows:

[0038] Peak No. 1: 0.57±0.01 (mangosteen alcohol)

[0039] Peak No. 2: 0.66±0.01 (3-isoman...

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Abstract

The invention discloses a mangosteen total xanthone extract and a preparation method thereof and well as application in the preparation of a TR3 receptor inducer, belonging to the field of pharmaceuticals and foods. The mangosteen total xanthone extract is applied to preparing foods or medicines for preventing or treating cancers, hepatitis or atherosclerosis as the TR3 receptor inducer. The TR3 receptor inducer and pharmaceutically-acceptable vectors are mixed to prepare powders, tablets, capsules, pills, suppositories, dropping pills, enteric-coated agents, injections, syrups, emulsions, suspensions, tinctures, ointments or sprays. Based on a mechanism that TR3 is possible to be an ideal molecular target for screening anti-tumor and cardiovascular medicines, the invention discovers and develops compounds for selectively controlling the TR3 expression approach or medicine compositions with the advanced treating characteristic and has practical meaning.

Description

technical field [0001] The invention belongs to the field of medicine and food, and in particular relates to a mangosteen total xanthone extract, a preparation method thereof and an application in preparing a TR3 receptor inducer. Background technique [0002] Mangosteen (Garcinia mangostana L.) is also known as mangosteen, mangosteen, mangosteen, and pineapple. It has a refreshing fragrance, smooth but not greasy, and is as famous as durian, known as the "queen of fruits". It is native to the Malaysian archipelago and has been introduced in most parts of southern China. Its main components are xanthones (xanthones) compounds represented by α-mangostin, β-mangostin and γ-mangostin (Journal of Natural Products, 1997, 60:519-524, Chemical and Pharmaceutical Bulletin, 2006, 54, 301-305). In Southeast Asia, mangosteen peel has been used for many years in traditional medicine for skin infections, wounds and diarrhea. The medicinal value of mangosteen has attracted the attentio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/185A23L1/30A61P1/16A61P9/10A61P35/00A61K131/00A23L33/105
Inventor 姚新生张晓坤戴毅王光辉段营辉陈海峰
Owner JINAN UNIVERSITY
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