The invention discloses a multiple detection method for six phospholipid in a complex sample. The multiple detection method includes HPLC analysis, wherein specification of a positive-phase silica gel chromatography column is 100mmx4.6mm, column temperature of 25 DEG C, elution flow speed is 1.5mL/min, flowing phase of gradient elution includes 40% of A, 57% of B and 3% of C of 0.0min, 40% of A, 50% of B and 10% of C of 8.0min, 40% of A, 50% of B and 10% of C of 15.0min, 40% of A, 57% of B and 3% of C of 15.1min and 40% of A, 57% of B and 3% of C of 24.0min, flowing phase A is normal hexane containing triethylamine of 0.01-0.08%, flowing phase B is isopropanol, and flowing phase C is acetic acid solution of 10-20%. The multiple detection method is simple in operation, quick, accurate, good in component separating effect, high in repeatability and easy for application and popularization in detection practice.