60 results about "Hplc analysis" patented technology

The invention belongs to the technical field of medicine detection, and particularly relates to a high-sensitivity liquid chromatography method for detecting mutagenic impurities in atomoxetine hydrochloride key starting material 3-chlorobenzene propanol, the method adopts an HPLC (High Performance Liquid Chromatography) analysis method, a chromatographic column adopts octadecylsilane chemically bonded silica as a filler, and the composition of a mobile phase A is a buffer solution; the mobile phase B is methanol or acetonitrile; carrying out gradient elution on the mobile phase; the flow velocity is 0.4 to 0.8 ml / min; the detection wavelength is 230 to 250 nm; by adopting an HPLC analysis method, the content of mutagenic impurities in the key starting material 3-chlorobenzene propanol of atomoxetine hydrochloride can be effectively separated and measured, and the method is high in sensitivity, strong in specificity and good in accuracy and repeatability, and has extremely important significance on quality control of the key starting material 3-chlorobenzene propanol of atomoxetine hydrochloride.

The invention relates to the technical field of intelligent control of HPLC (High Performance Liquid Chromatography) analytical instruments, in particular to an HPLC system fault early warning self-calibration method. Comprising the following steps: S1, acquiring a high-frequency pressure signal behind a pump and a chromatographic signal of a detector in real time; calculating a relative physical disturbance quantity based on the post-pumping high-frequency pressure signal; calculating a chemical state deviation degree based on the detector chromatographic signal; s2, fusing the relative physical disturbance quantity and the chemical state deviation degree, and constructing an instantaneous health index; and S3, comparing the instantaneous health index with a preset first early warning threshold value and a preset second early warning threshold value, and executing a closed-loop control strategy corresponding to a comparison result. According to the method, accurate fault early warning is realized by constructing a physical-chemical two-dimensional monitoring system, the defects of offline and lagging applicability test of a traditional system are overcome through real-time monitoring and fusion evaluation of the two dimensions, and the accuracy and timeliness of early warning are remarkably improved.

The purpose of the present invention is to provide a beer-taste alcoholic beverage having a rich flavor, a favorable aftertaste, and a favorable amount of flavor. The present invention provides: a beer-taste alcoholic beverage in which the total concentration of free carboxymethyllysine (CML), free carboxyethyllysine (CEL), and free methylglyoxal-derived hydroimidazolone-1 (MG-H1) is 280-1,400 ppb; and a beer-taste alcoholic beverage in which the total concentration of free CML, free CEL, and free MG-H1 is 280-1,600 ppb, the total concentration of bound CML, bound CEL, and bound MG-H1 is 150-900 ppb, and the bound CML, the bound CEL, and the bound MG-H1 are included in a peptide fraction having a molecular weight of 1,060-3,080 Da (gel filtration method for HPLC analysis).

The invention discloses a method for simultaneously extracting and separating flavonoid and alkaloid components in mulberry leaves, which comprises the following steps: firstly, adjusting the pH value of an aqueous solution of a deep eutectic solvent to form a uniform single-phase solution, adding mulberry leaf powder into the single-phase solution, carrying out ultrasonic extraction, centrifuging after the extraction is finished, and taking a supernatant; adjusting the pH value of the extracting solution again to form a two-phase system, and respectively obtaining an upper-layer eutectic solvent phase and a lower-layer water phase. The conditions of the extraction step are as follows: the concentration of the eutectic solvent is 30%-70%, the solid-to-liquid ratio is 1: 5 g / mL-1: 40 g / mL, the extraction time is 20 min-100 min, and the extraction pH is 5.5-9.5; in the separation step, the conditions of the separation step are as follows: the separation pH is 1-5. Through HPLC (High Performance Liquid Chromatography) analysis, a flavonoid component (isoquercitrin) is mainly distributed in an upper-layer deep-eutectic solvent phase, an alkaloid component (deoxynojirimycin) is mainly distributed in a lower-layer water phase, and the reusability is good.

The invention discloses a method for determining the solution concentration in the crystallization process of 3, 4-dinitrofurazan furoxan oxide, and relates to the technical field of energetic crystal materials. According to the method, an EasySampler and HPLC (High Performance Liquid Chromatography) combined method is utilized, the solubility of DNTF is measured, a standard curve of DNTF solution concentration and peak area is established by utilizing a high performance liquid chromatograph, then the EasySampler is utilized to sample a crystallization process and perform HPLC analysis, and then the solution concentration at each moment is calculated by utilizing an equation of the standard curve. According to the method, EasySampler equal interval sampling is combined with off-line HPLC (High Performance Liquid Chromatography) analysis, and a curve that the concentration of the main component of the solution changes along with the temperature is constructed. In the dominant period of crystal growth, the concentration change rate and the solid-liquid interface area are in an approximately linear relationship. The experimental data provides a necessary demonstration basis for subsequent improvement of a non-ideal system crystallization kinetics theory.

The objective is to provide 5-aminolevulinic acid or its salts and a method for producing the same, which have fewer impurities and residual solvents and suppressed discoloration compared to conventional methods. The objective is to provide 5-aminolevulinic acid or its salts, wherein, based on peak area obtained by HPLC analysis, the ratio of the content of individual impurities to the content of 5-aminolevulinic acid or its salt is 0.0007 or less, and the sum of the ratios of the content of individual impurities to the content of 5-aminolevulinic acid or its salt is 0.0016 or less.

A chromatographic system for the simultaneous RP-HPLC quantification of aloin and curcumin, consisting of a C18 column, a PDA detector, a programmable solvent feed module and an optimized mobile phase of acetonitrile, methanol and water.

The present disclosure provides methods useful for the preparation of compounds of Formula I: or a pharmaceutically acceptable salt thereof, and pharmaceutical compositions comprising compounds of Formula I wherein Michael addition impurities are present in an amount of less than 0.3%, as determined by HPLC analysis, as well as methods of using compounds of Formula I and pharmaceutical compositions in the treatment of cancer.

To provide a process for purifying an antibody or a fusion protein from a protein mixture containing product - and process-related impurities.SOLUTION: Provided is a process for purifying a protein of interest from a protein mixture comprising the protein of interest and one or more low molecular weight (LMW) variants, comprising: a. loading the protein mixture onto a ceramic hydroxyapatite (CHT) column with a suitable buffer; b. washing the CHT column with a suitable buffer; and c. eluting the purified protein of interest with a suitable gradient of phosphate buffer, wherein the protein of interest is substantially pure and has less than 0.4% LMW as analyzed by SE-HPLC analysis.SELECTED DRAWING: None

The invention belongs to chemical detection of textiles, provides a method for detecting polyhexamethylene biguanide hydrochloride in textiles, and belongs to the technical field of chemical detection. According to the method, ultrasonic extraction is adopted for sample pretreatment, and the content of polyhexamethylene biguanide hydrochloride in the textile is detected by optimizing chromatographic conditions in HPLC (High Performance Liquid Chromatography) analysis. According to the detection method, the content of the polyhexamethylene biguanide hydrochloride in the textile can be simply, accurately and sensitively measured, and the quality of textile products is controlled, so that smooth export of the textile products in China is effectively guaranteed.

The invention discloses an analysis method of 2-chloro-1,3-bis(dimethylamino)trimethylene hexafluorophosphate genotoxic impurities in etoricoxib, adopting an HPLC analysis method, wherein the following are adopted: the instrument is a high performance liquid chromatograph with an ultraviolet detector; mobile phase: A: phosphate and ion pair reagent buffer, B: methanol; gradient elution; detection wavelength: 310nm; column temperature: 30-40°C; injection volume: 10μl; solvent is: mobile phase A: B=30:70; test solution: concentration is 10mg / ml; reference solution: concentration is 0.125μg / ml; determination process: blank solution, reference solution and test solution are respectively sampled and analyzed in sequence; content is calculated by peak area according to an external standard method. The analysis method of the invention can be analyzed by conventional liquid chromatography method when the limit of genotoxic impurities in the drug is low, i.e., the determination is accurate, thereby reducing the detection cost.

To provide an epoxy resin which is excellent in storage stability and gives a cured product excellent in flexural strength and toughness, a curable resin composition, and a cured product thereof.SOLUTION: An epoxy resin represented by the following formula (1), wherein (b + c) / a is 1% or more and less than 20% when a peak area in a case where X in the formula (1) is (1-a) is a, a peak area in a case where X in the formula (1) is (1-b) is b, and a peak area in a case where X in the formula (1) is (1-c) is c in HPLC analysis.SELECTED DRAWING: None

To provide a novel beer-taste non-alcoholic beverage in which cloying taste and flavor are reduced and beer-like qualities are achieved in good balance.SOLUTION: There is provided a beer-taste non-alcoholic beverage containing malt as at least a part of raw material, where the concentration of peptide having a molecular weight of 800 to 4,600 Da (a gel filtration method for HPLC analysis) in the beverage, as fractionated by the gel filtration method for HPLC analysis, is 0.25 mg / mL or more and 3.00 mg / mL or less.SELECTED DRAWING: None

The invention relates to a liquid chromatogram tail liquid collecting device, which can accurately collect each chromatographic peak in HPLC (High Performance Liquid Chromatography) analysis by setting delay dead time, and has the advantages of small diffusion, small volume, low cost and the like.

The purpose of the present invention is to provide a novel beer-flavored beverage that achieves both lightness and beer-like satisfaction. According to the present invention, provided is a beer-flavored beverage in which: the total concentration of free-form CML, free-form CEL, and free-form MG-H1 is 55 ppb to 500 ppb; the total concentration of bound-form CML, bound-form CEL, and bound-form MG-H1 is 50 ppb to 650 ppb; the bound-form CML, the bound-form CEL, and the bound-form MG-H1 are contained in a peptide fraction having a molecular weight of 1,060 Da to 3,080 Da (gel filtration method for HPLC analysis); and the total nitrogen concentration is 500 ppm or less. According to the present invention, it is possible to provide a beer-flavored beverage that achieves both lightness and beer-like satisfaction and is advantageous in meeting various consumer preferences and needs.

A polythiol composition according to exemplary embodiments includes a first polythiol compound which provides a maximum peak in a high performance liquid chromatography (HPLC) analysis graph obtained at a wavelength of 230 nm, and a second polythiol compound having a molecular weight greater than that of the first polythiol compound and represented by C9H20S6. A ratio of a peak area of the second polythiol compound to a peak area of the first polythiol compound, which are measured through the HPLC analysis graph at the wavelength of 230 nm, is 0.05 to 5.0%.

The present disclosure provides processes that can be used to prepare the compound of Formula I:, or pharmaceutically acceptable salts thereof, and pharmaceutical compositions comprising the compound of Formula I, wherein a Michael adduct impurity is present in an amount of less than 0.3% as measured by HPLC Analysis, and methods of treating cancer using the compound of Formula I and pharmaceutical compositions.

The present application relates to the technical field of intelligent control of HPLC analysis instrument, in particular to a HPLC system fault early warning self-calibration method, comprising: S1, collecting pump post high-frequency pressure signals and detector chromatogram signals in real time; based on the pump post high-frequency pressure signals, calculating relative physical disturbance quantity; based on the detector chromatogram signals, calculating chemical state deviation degree; S2, fusing the relative physical disturbance quantity and the chemical state deviation degree to construct an instantaneous health index; S3, comparing the instantaneous health index with a preset first early warning threshold and a second early warning threshold, and executing a closed-loop control strategy corresponding to the comparison result. The present method realizes accurate early fault warning by constructing a physical-chemical dual-dimension monitoring system, and the real-time monitoring and fusion evaluation of this dual-dimension overcome the defects of offline and lag of traditional system applicability test, and significantly improve the accuracy and timeliness of early warning.

The invention discloses a qualitative and quantitative detection method of a grifola frondosa polysaccharide D-component, which comprises the following steps: selecting defatted grifola frondosa powder from all over the country, extracting crude polysaccharide, carrying out HPLC (High Performance Liquid Chromatography) analysis and detection, and judging the existence of the grifola frondosa polysaccharide D-component according to the chromatographic retention time. Then analyzing monosaccharide composition and amino acid composition of the purified grifola frondosa polysaccharide D-component so as to further determine the composition range of monosaccharide and amino acid in the grifola frondosa polysaccharide D-component, and then detecting the HPLC peak area of the pure grifola frondosa polysaccharide D-component by HPLC analysis so as to measure the content of the grifola frondosa polysaccharide D-component.

The invention discloses an HPLC (High Performance Liquid Chromatography) analysis method for an impurity I in nicergoline tablets. The HPLC analysis method comprises the following steps: 1) preparing a diluent, a test solution, a reference solution and a blank auxiliary solution; (2) testing by using chromatography, wherein a mobile phase is a buffer solution (1 ml of triethylamine, 1000 ml of water, uniformly mixing, and adjusting the pH value to 2.4 by using phosphoric acid)-acetonitrile (70: 30); the column temperature is 30 DEG C, the flow rate is 1.0 ml / min, the detection wavelength is 228 nm, the sample injection volume is 10 [mu] l, and the operation time is 35 min. According to the method, the impurity I in the nicergoline tablet can be independently analyzed, the detection problem of the impurity I in the nicergoline tablet production process is solved, the content of the impurity I in the nicergoline tablet is accurately detected, a producer can compare with the quality of an original research medicine in the consistency evaluation process, and the quality of the product is improved. The method is of great significance in improving the product quality of tablets and improving the medication safety of patients.

The purpose of the present invention is to provide a novel beer-taste non-alcoholic beverage in which cloying taste and flavor are minimized and beer-like qualities are achieved in good balance. According to the present invention, there is provided a beer-taste non-alcoholic beverage containing malt as at least a portion of raw materials, wherein the concentration of a peptide having a molecular weight of 800-4600 Da (a gel filtration method for HPLC analysis) in the beverage, as fractionated by a gel filtration method for HPLC analysis, is 0.25-3.00 mg / mL.

The invention discloses streptomyces parvus, a microbial agent and application of the streptomyces parvus and the microbial agent, and relates to the technical field of environmental microorganisms. A strain of streptomyces PS-15 with excellent application potential is separated from soil of the Tibet Plateau, and the strain belongs to streptomyces and deep olive streptomyces through identification. The strain has antibacterial activity, HPLC (high performance liquid chromatography) analysis of a fermentation product of the strain shows that the abundance of a main product is high, the main product is bernin amycin C, and the strain has the characteristic of high yield of bernin amycin C and is expected to be used for industrial production of bernin amycin C and preparation of antibacterial agents.

The present invention discloses a fluxapyroxad synthesis method, and relates to the technical field of organic chemical synthesis, and the fluxapyroxad synthesis method comprises: preparing raw materials including fluorinated benzene, an amide source and a solvent; adding a catalyst, and carrying out acylation reaction on fluorinated benzene and an amide source by using an ultrasonic technology to obtain an intermediate; heating the obtained intermediate by microwave radiation, and cyclizing to form fluxapyroxad; separating the fluxapyroxad by using a supercritical fluid extraction method; and carrying out NMR and HPLC analysis on the obtained product, and analyzing the structure and purity of the product. According to the method, the acylation reaction of the fluorinated benzene and the amide source is promoted through the ultrasonic technology, the reaction rate and the reaction conversion rate can be remarkably increased, the synthesis time can be shortened, the fluxapyroxad is separated through the supercritical fluid extraction technology, the influence on the environment is reduced, the supercritical fluid has good dissolving capacity, and the yield of the fluxapyroxad is improved. The target product can be efficiently separated, and meanwhile, waste is reduced.

[Objective] The purpose of this invention is to provide a simple, efficient, and scalable purification method for tafluprost. [Solution] This invention relates to a purification method for tafluprost, comprising the steps of purifying crude tafluprost using silica gel column chromatography and collecting the tafluprost-containing component using HPLC analysis. Furthermore, this invention also relates to a method for manufacturing tafluprost incorporating the aforementioned purification method.

The invention relates to the technical field of drug detection, and particularly discloses a method for detecting related substances in cycloserine. The specific derivatization reagent is adopted, so that the impurities and the benzaldehyde derivative are subjected to derivatization reaction to generate amide, and the ultraviolet absorption response intensity of the impurities is improved; according to the method, a ZORBAX SB-Phenyl chromatographic column is adopted to carry out HPLC (High Performance Liquid Chromatography) analysis on a cycloserine sample, so that rapid and accurate detection on impurities such as D-serine, D-serine methyl ester hydrochloride, 3-chloro-D-alanine methyl ester hydrochloride and L-cycloserine in the cycloserine at the same time is realized. The detection method is high in specificity, low in detection limit and quantitation limit, good in linear relation, high in recovery rate, good in repeatability, high in stability, good in durability, easy and rapid to operate, low in detection cost and accurate and reliable in detection result, can be used for quality control and comprehensive evaluation of cycloserine, and provides reliable guarantee for improving and controlling the quality of cycloserine.

The invention discloses a synthetic method of 5-aminolevulinic acid and derivatives thereof, 5-aminolevulinic acid and derivatives thereof and application, and relates to the technical field of chemical synthesis, and the synthetic method comprises the following steps: mixing monoalkyl succinate, an inert solvent and an activating reagent, and carrying out acylation activating reaction to obtain a first intermediate; mixing the first intermediate, nitromethane, an alkaline agent, a molecular sieve and an organic solvent, and carrying out nitro condensation reaction to obtain a second intermediate; and mixing the second intermediate, a solvent and a catalyst, and carrying out catalytic hydrogenation reaction to obtain the 5-aminolevulinic acid and the derivative thereof. By adopting the steps, only three-step reaction is needed from the initial raw material to the target product, the reaction conditions are mild and efficient, the 5-aminolevulinic acid and the derivative thereof prepared by the process are white solids, the chemical purity of the 5-aminolevulinic acid and the derivative thereof can reach more than 96.0% and the yield can reach more than 70% through HPLC (High Performance Liquid Chromatography) analysis, and the requirements of industrial large-scale production are completely met.

The invention discloses a preparation method of a vegetable dye and a dyeing process. The preparation method comprises the following steps: S1, raw material selection: selecting a Suzuki plant as a raw material; s2, low-temperature extraction: extracting a crude dye containing dihydroquercetin or quercetin by using a low-temperature extraction method; s3, collecting an extracting solution: collecting the extracting solution into a clean container; s4, filtering: removing insoluble impurities by using filter paper or other proper filter media; s5, primary separation: further removing macromolecular impurities and other non-target components by using a membrane separation technology; s6, purification and analysis: performing HPLC (High Performance Liquid Chromatography) analysis on the filtered extracting solution, and determining the initial content of dihydroquercetin or quercetin; and S7, refining and purifying: further separating and enriching target components by using column chromatography or thin layer chromatography. The method shows good dyeing compatibility for fabrics made of various materials, covers natural fiber fabrics, chemical fiber fabrics and various mixed fiber fabrics, and greatly expands the application range of the fabrics.

The invention provides a method for improving the accuracy of vancomycin plasma concentration monitored by HPLC (High Performance Liquid Chromatography) based on PDCA (Packet Data Convergence Analysis) circulation, and belongs to the technical field of quantitative analysis. When the clinical sample is abnormal, retesting the abnormal clinical sample to obtain a retesting result, and judging whether interference exists in the retesting sample or not according to the retesting result; when the existence of interference in the retest sample is still not determined, quantifying the vancomycin plasma concentration level in the retest sample based on a liquid chromatography-mass spectrometry method to obtain a liquid chromatography-mass spectrometry method quantitative result; and outputting the quantitative result of the liquid chromatography-mass spectrometry method when the quantitative result of the liquid chromatography-mass spectrometry method is inconsistent with the determination result of the high performance liquid chromatography. The method has the beneficial effects that through continuous application of PDCA circulation, the accuracy of vancomycin plasma concentration monitoring by the vancomycin HPLC analysis method is continuously improved, and the quality and accuracy of vancomycin TDM results are ensured.

The application relates to the field of vaccine detection, and specifically discloses a cell influenza virus split vaccine and a detection method for the content of recombinant trypsin in the production process of the cell influenza virus split vaccine. The method comprises the following steps: taking the influenza virus split vaccine or an intermediate product in the production process of the influenza virus split vaccine for pretreatment to obtain a test sample solution; taking the recombinant trypsin as a control sample to prepare a control sample solution; performing HPLC analysis on the control sample solution and the test sample solution; and calculating the content of the recombinant trypsin in the test sample solution by using an external standard method. The detection method has strong specificity and small interference; the detection limit is 0.5 ug / ml, the method has high sensitivity; and the linear relationship is good within the concentration range of 10-45 ug / ml, which is helpful for rapidly detecting the content of the recombinant trypsin in the influenza virus split vaccine and a culture system in the production process of the vaccine, so as to further improve the production efficiency and biological safety of the influenza virus split vaccine.

The application discloses a method for simultaneously extracting and separating flavonoids and alkaloids in mulberry leaves, which comprises the following steps: firstly, adjusting the pH of a eutectic solvent aqueous solution to form a homogeneous single-phase solution; then adding mulberry leaf powder into the solution and performing ultrasonic extraction; after the extraction is completed, centrifuging and taking the supernatant; and secondly, adjusting the pH of the extraction solution to form a two-phase system, and obtaining an upper eutectic solvent phase and a lower water phase respectively. The extraction step conditions are as follows: the eutectic solvent concentration is 30%-70%, the solid-liquid ratio is 1:5 g / mL-1:40 g / mL, the extraction time is 20 min-100 min, and the extraction pH is 5.5-9.5; in the separation step, the separation step conditions are as follows: the separation pH is 1-5. Through HPLC analysis, flavonoids (isoquercitrin) are mainly distributed in the upper eutectic solvent phase, and alkaloids (deoxyacteoside) are mainly distributed in the lower water phase, and the reusability is good.