26 results about "Hplc analysis" patented technology

The purpose of the present invention is to provide a beer-taste alcoholic beverage having a rich flavor, a favorable aftertaste, and a favorable amount of flavor. The present invention provides: a beer-taste alcoholic beverage in which the total concentration of free carboxymethyllysine (CML), free carboxyethyllysine (CEL), and free methylglyoxal-derived hydroimidazolone-1 (MG-H1) is 280-1,400 ppb; and a beer-taste alcoholic beverage in which the total concentration of free CML, free CEL, and free MG-H1 is 280-1,600 ppb, the total concentration of bound CML, bound CEL, and bound MG-H1 is 150-900 ppb, and the bound CML, the bound CEL, and the bound MG-H1 are included in a peptide fraction having a molecular weight of 1,060-3,080 Da (gel filtration method for HPLC analysis).

The objective is to provide 5-aminolevulinic acid or its salts and a method for producing the same, which have fewer impurities and residual solvents and suppressed discoloration compared to conventional methods. The objective is to provide 5-aminolevulinic acid or its salts, wherein, based on peak area obtained by HPLC analysis, the ratio of the content of individual impurities to the content of 5-aminolevulinic acid or its salt is 0.0007 or less, and the sum of the ratios of the content of individual impurities to the content of 5-aminolevulinic acid or its salt is 0.0016 or less.

A chromatographic system for the simultaneous RP-HPLC quantification of aloin and curcumin, consisting of a C18 column, a PDA detector, a programmable solvent feed module and an optimized mobile phase of acetonitrile, methanol and water.

The present disclosure provides methods useful for the preparation of compounds of Formula I: or a pharmaceutically acceptable salt thereof, and pharmaceutical compositions comprising compounds of Formula I wherein Michael addition impurities are present in an amount of less than 0.3%, as determined by HPLC analysis, as well as methods of using compounds of Formula I and pharmaceutical compositions in the treatment of cancer.

To provide a process for purifying an antibody or a fusion protein from a protein mixture containing product - and process-related impurities.SOLUTION: Provided is a process for purifying a protein of interest from a protein mixture comprising the protein of interest and one or more low molecular weight (LMW) variants, comprising: a. loading the protein mixture onto a ceramic hydroxyapatite (CHT) column with a suitable buffer; b. washing the CHT column with a suitable buffer; and c. eluting the purified protein of interest with a suitable gradient of phosphate buffer, wherein the protein of interest is substantially pure and has less than 0.4% LMW as analyzed by SE-HPLC analysis.SELECTED DRAWING: None

To provide an epoxy resin which is excellent in storage stability and gives a cured product excellent in flexural strength and toughness, a curable resin composition, and a cured product thereof.SOLUTION: An epoxy resin represented by the following formula (1), wherein (b + c) / a is 1% or more and less than 20% when a peak area in a case where X in the formula (1) is (1-a) is a, a peak area in a case where X in the formula (1) is (1-b) is b, and a peak area in a case where X in the formula (1) is (1-c) is c in HPLC analysis.SELECTED DRAWING: None

The purpose of the present invention is to provide a novel beer-flavored beverage that achieves both lightness and beer-like satisfaction. According to the present invention, provided is a beer-flavored beverage in which: the total concentration of free-form CML, free-form CEL, and free-form MG-H1 is 55 ppb to 500 ppb; the total concentration of bound-form CML, bound-form CEL, and bound-form MG-H1 is 50 ppb to 650 ppb; the bound-form CML, the bound-form CEL, and the bound-form MG-H1 are contained in a peptide fraction having a molecular weight of 1,060 Da to 3,080 Da (gel filtration method for HPLC analysis); and the total nitrogen concentration is 500 ppm or less. According to the present invention, it is possible to provide a beer-flavored beverage that achieves both lightness and beer-like satisfaction and is advantageous in meeting various consumer preferences and needs.

The present application relates to the technical field of intelligent control of HPLC analysis instrument, in particular to a HPLC system fault early warning self-calibration method, comprising: S1, collecting pump post high-frequency pressure signals and detector chromatogram signals in real time; based on the pump post high-frequency pressure signals, calculating relative physical disturbance quantity; based on the detector chromatogram signals, calculating chemical state deviation degree; S2, fusing the relative physical disturbance quantity and the chemical state deviation degree to construct an instantaneous health index; S3, comparing the instantaneous health index with a preset first early warning threshold and a second early warning threshold, and executing a closed-loop control strategy corresponding to the comparison result. The present method realizes accurate early fault warning by constructing a physical-chemical dual-dimension monitoring system, and the real-time monitoring and fusion evaluation of this dual-dimension overcome the defects of offline and lag of traditional system applicability test, and significantly improve the accuracy and timeliness of early warning.

The invention discloses an HPLC (High Performance Liquid Chromatography) analysis method for an impurity I in nicergoline tablets. The HPLC analysis method comprises the following steps: 1) preparing a diluent, a test solution, a reference solution and a blank auxiliary solution; (2) testing by using chromatography, wherein a mobile phase is a buffer solution (1 ml of triethylamine, 1000 ml of water, uniformly mixing, and adjusting the pH value to 2.4 by using phosphoric acid)-acetonitrile (70: 30); the column temperature is 30 DEG C, the flow rate is 1.0 ml / min, the detection wavelength is 228 nm, the sample injection volume is 10 [mu] l, and the operation time is 35 min. According to the method, the impurity I in the nicergoline tablet can be independently analyzed, the detection problem of the impurity I in the nicergoline tablet production process is solved, the content of the impurity I in the nicergoline tablet is accurately detected, a producer can compare with the quality of an original research medicine in the consistency evaluation process, and the quality of the product is improved. The method is of great significance in improving the product quality of tablets and improving the medication safety of patients.

[Objective] The purpose of this invention is to provide a simple, efficient, and scalable purification method for tafluprost. [Solution] This invention relates to a purification method for tafluprost, comprising the steps of purifying crude tafluprost using silica gel column chromatography and collecting the tafluprost-containing component using HPLC analysis. Furthermore, this invention also relates to a method for manufacturing tafluprost incorporating the aforementioned purification method.

The invention discloses a synthetic method of 5-aminolevulinic acid and derivatives thereof, 5-aminolevulinic acid and derivatives thereof and application, and relates to the technical field of chemical synthesis, and the synthetic method comprises the following steps: mixing monoalkyl succinate, an inert solvent and an activating reagent, and carrying out acylation activating reaction to obtain a first intermediate; mixing the first intermediate, nitromethane, an alkaline agent, a molecular sieve and an organic solvent, and carrying out nitro condensation reaction to obtain a second intermediate; and mixing the second intermediate, a solvent and a catalyst, and carrying out catalytic hydrogenation reaction to obtain the 5-aminolevulinic acid and the derivative thereof. By adopting the steps, only three-step reaction is needed from the initial raw material to the target product, the reaction conditions are mild and efficient, the 5-aminolevulinic acid and the derivative thereof prepared by the process are white solids, the chemical purity of the 5-aminolevulinic acid and the derivative thereof can reach more than 96.0% and the yield can reach more than 70% through HPLC (High Performance Liquid Chromatography) analysis, and the requirements of industrial large-scale production are completely met.

The invention provides a method for improving the accuracy of vancomycin plasma concentration monitored by HPLC (High Performance Liquid Chromatography) based on PDCA (Packet Data Convergence Analysis) circulation, and belongs to the technical field of quantitative analysis. When the clinical sample is abnormal, retesting the abnormal clinical sample to obtain a retesting result, and judging whether interference exists in the retesting sample or not according to the retesting result; when the existence of interference in the retest sample is still not determined, quantifying the vancomycin plasma concentration level in the retest sample based on a liquid chromatography-mass spectrometry method to obtain a liquid chromatography-mass spectrometry method quantitative result; and outputting the quantitative result of the liquid chromatography-mass spectrometry method when the quantitative result of the liquid chromatography-mass spectrometry method is inconsistent with the determination result of the high performance liquid chromatography. The method has the beneficial effects that through continuous application of PDCA circulation, the accuracy of vancomycin plasma concentration monitoring by the vancomycin HPLC analysis method is continuously improved, and the quality and accuracy of vancomycin TDM results are ensured.

The application relates to the field of vaccine detection, and specifically discloses a cell influenza virus split vaccine and a detection method for the content of recombinant trypsin in the production process of the cell influenza virus split vaccine. The method comprises the following steps: taking the influenza virus split vaccine or an intermediate product in the production process of the influenza virus split vaccine for pretreatment to obtain a test sample solution; taking the recombinant trypsin as a control sample to prepare a control sample solution; performing HPLC analysis on the control sample solution and the test sample solution; and calculating the content of the recombinant trypsin in the test sample solution by using an external standard method. The detection method has strong specificity and small interference; the detection limit is 0.5 ug / ml, the method has high sensitivity; and the linear relationship is good within the concentration range of 10-45 ug / ml, which is helpful for rapidly detecting the content of the recombinant trypsin in the influenza virus split vaccine and a culture system in the production process of the vaccine, so as to further improve the production efficiency and biological safety of the influenza virus split vaccine.

The application discloses a method for simultaneously extracting and separating flavonoids and alkaloids in mulberry leaves, which comprises the following steps: firstly, adjusting the pH of a eutectic solvent aqueous solution to form a homogeneous single-phase solution; then adding mulberry leaf powder into the solution and performing ultrasonic extraction; after the extraction is completed, centrifuging and taking the supernatant; and secondly, adjusting the pH of the extraction solution to form a two-phase system, and obtaining an upper eutectic solvent phase and a lower water phase respectively. The extraction step conditions are as follows: the eutectic solvent concentration is 30%-70%, the solid-liquid ratio is 1:5 g / mL-1:40 g / mL, the extraction time is 20 min-100 min, and the extraction pH is 5.5-9.5; in the separation step, the separation step conditions are as follows: the separation pH is 1-5. Through HPLC analysis, flavonoids (isoquercitrin) are mainly distributed in the upper eutectic solvent phase, and alkaloids (deoxyacteoside) are mainly distributed in the lower water phase, and the reusability is good.

The application discloses application of 2-ketobutyric acid, and finds that the 2-ketobutyric acid can obviously see the bacteriostatic circle on an aspergillus flavus plate at 2 mg. After the aspergillus flavus is added with 2-ketobutyric acid at 2 mg / mL, and after 24 and 48 hours of culture, the 2-ketobutyric acid can effectively achieve a bacteriostatic rate of more than 90%. Iodine propidium (PI) staining shows that the 2-ketobutyric acid treatment has damage to a fungal cell membrane. Transcriptome analysis shows that the 2-ketobutyric acid can down-regulate gene expression of aspergillus flavus biosynthesis. HPLC analysis can also show that after the aspergillus flavus is treated by the 2-ketobutyric acid, aspergillus flavus is obviously decreased relative to a control group.

This invention discloses a liquid chromatography method for the simultaneous detection of dibutyllauroyl glutamine raw material, intermediate, and product, belonging to the field of chemical analysis. The method involves HPLC analysis of a reaction solution containing dibutyllauroyl glutamine raw material, intermediate, and product. The raw material is lauroyl glutamic acid; the intermediate is lauroyl glutamic acid trifluoroethyl ester; and the product is dibutyllauroyl glutamine. The HPLC chromatographic conditions are as follows: a C18 column; a mobile phase consisting of a mixture of disodium hydrogen phosphate solution and acetonitrile at a volume ratio of (30-10):(70-90); and isocratic elution. This invention achieves effective separation of the raw material lauroyl glutamic acid, the trifluoroethyl ester intermediate, and the product in the synthesis reaction of dibutyllauroyl glutamine, exhibiting good peak shape, high sensitivity, and accuracy.

This application relates to the technical field of chemical detection, and in particular, to chemical detection of textiles, and more specifically, provides a method for detecting polyhexamethylene biguanide hydrochloride in textiles. The method employs ultrasonic extraction for sample pretreatment, and optimizes chromatographic conditions in HPLC analysis to detect the content of the polyhexamethylene biguanide hydrochloride in a textile. The detection method of this application enables easy, accurate, and sensitive determination of the content of the polyhexamethylene biguanide hydrochloride in textiles and facilitates quality control of textile products, thereby effectively ensuring smooth export of textile products.

The invention discloses a calanthe japonica flower color and flower fragrance component analysis method, and belongs to the technical field of plant component analysis. The method comprises the following steps: S1, synchronous collection and pretreatment of samples: collecting flower buds and flowers of the same scapes, respectively taking petals and lips, and uniformly dividing the petals and lips into flower fragrance and pigment detection samples; s2, targeted extraction and GC-MS (gas chromatography-mass spectrometry) detection of flower fragrance components: extracting by adopting headspace solid-phase microextraction combined with an internal standard method, and performing GC-MS analysis by optimizing a heating program; s3, high-efficiency extraction and HPLC (High Performance Liquid Chromatography) detection of the anthocyanidin: carrying out ultrasonic extraction by adopting a composite solvent containing vitamin C, and carrying out HPLC analysis through gradient elution; and S4, data processing and dynamic correlation analysis: performing qualitative and quantitative analysis on a detection result. Through targeted sampling and composite extraction technologies, the problems of high sample consumption, long period and lack of data relevance in a traditional method are solved, accurate analysis of the flower color and flower fragrance components of the calanthe plants is realized, and a key technical support is provided for gardening breeding.

The invention provides a separation and purification method for cis-1, 3-dibenzylimidazole-2-ketone-4, 5-dicarboxylic acid in a cyclic acid crude product. The separation and purification method comprises the following steps: S1, dissolving a cyclic acid crude product in an ethanol solution to obtain a mixed solution; s2, dropwise adding a sodium hydroxide solution into the mixed solution to adjust the pH value to 12.8-13.2, carrying out an isomerization reaction, then adjusting the pH value to 6.3-6.7, and filtering to obtain a clear filtrate; s3, calcium salt is added into the clarified filtrate for selective precipitation, and metal salt precipitation is obtained; s4, mixing and pulping the metal salt precipitate and water, adding hydrochloric acid for acidification, filtering, and washing a filter cake with cold water to obtain a cis-cyclic acid crude product; and S5, dissolving the cis-cyclic acid crude product in a mixed solvent of acetone and water, dropwise adding an alkaline solution to adjust the pH value to 4.0-4.8, cooling, crystallizing, and drying in vacuum to obtain the high-purity cis-cyclic acid. According to HPLC analysis, the purity of the product obtained through purification reaches 99% or above, trans-isomer impurities are strictly controlled to be 0.5% or below, and the product is in a regular crystal form and is good in consistency.

The purpose of the present invention is to provide 5-aminolevulinic acid or a salt thereof and a method for producing the same, the 5-aminolevulinic acid or salt thereof having suppressed discoloration and fewer impurities and residual solvents than in the prior art. Provided is 5-aminolevulinic acid or a salt thereof, in which the ratio of the content of individual impurities to the content of the 5-aminolevulinic acid or salt thereof is 0.0007 or less, and the total of the ratios of the contents of the individual impurities to the content of the 5-aminolevulinic acid or salt thereof is 0.0016 or less, based on the peak areas obtained by HPLC analysis.

The application provides a detection method for determining methionine and impurities thereof by using high performance liquid chromatography, comprising the following steps: A) dissolving a sample to be detected by using water to obtain a test sample solution; B) determining the test sample solution by using high performance liquid chromatography to obtain a chromatogram of the test sample solution; and chromatographic parameters are as follows: a chromatographic column is an XB-SCX column; a mobile phase is an isocratic elution; and the mobile phase is a water solution containing 0.09%-0.11% formic acid and 0.09%-0.11% diethylamine. The application determines methionine and impurities thereof by using HPLC analysis and detection spectrum, and other components in the test sample solution do not interfere with the detection of methionine sulfoxide and serine; the application can achieve the purpose of rapidly, effectively and simultaneously detecting methionine sulfoxide and serine in methionine, and has good specificity, durability and high detection sensitivity.

[Objective] The purpose of this invention is to provide a simple, efficient, and scalable purification method for tafluprost. [Solution] This invention relates to a purification method for tafluprost, comprising the steps of purifying crude tafluprost using silica gel column chromatography and collecting the tafluprost-containing component using HPLC analysis. Furthermore, this invention also relates to a method for manufacturing tafluprost incorporating the aforementioned purification method.

[Objective] The purpose of this invention is to provide a simple, efficient, and scalable purification method for tafluprost. [Solution] This invention relates to a purification method for tafluprost, comprising the steps of purifying crude tafluprost using silica gel column chromatography and collecting the tafluprost-containing component using HPLC analysis. Furthermore, this invention also relates to a method for manufacturing tafluprost incorporating the aforementioned purification method.

The invention relates to a method for rapidly detecting auramine in bean products, and belongs to the technical field of food detection. The method for detecting the auramine in the bean product mainly utilizes HPLC (High Performance Liquid Chromatography) for detection, and comprises the steps of pretreatment, reference substance solution preparation, HPLC analysis and the like, and in the sample pretreatment process, the auramine in the bean product is fully extracted by adopting the modes of combining enzymolysis with ultrasound, combining an organic solvent with ultrasound and the like. The method provided by the invention can be used for effectively extracting and detecting the auramine in the bean product, so that the detection accuracy is greatly improved, and the food safety is ensured.

[Problem] The present application aims to provide a simple and efficient method for purifying tafluprost, which can be scaled up. [Means for solving the problem] The present application relates to a method for purifying tafluprost, which includes purifying a crude product of tafluprost using a silica gel column chromatography and collecting a component containing tafluprost using HPLC analysis. In addition, the present application also relates to a method for producing tafluprost including the aforementioned method for purifying tafluprost.

The invention discloses a Maotai-flavor liquor cellar bottom complex ester content detection device and a method thereof, and relates to the technical field of liquor brewing. The device comprises a sampling assembly (a multi-depth sampling pipe, a negative pressure pump and a mixing tank), an extraction assembly (a constant temperature tank and a quantitative dosing device), a detection assembly (a filtering module, a high performance liquid chromatograph and a data processing module) and a fixing assembly. The method comprises the steps of sampling (multi-region mixed sampling), pretreatment (filtering), extraction (precise temperature control and dosing), detection (HPLC analysis) and data processing (automatic content calculation). The method solves the problems of poor sampling representativeness, large extraction error and low efficiency in existing detection, the detection error is smaller than or equal to + / -2%, the single sample detection time is 40-60 minutes, starter formula screening and fermentation temperature optimization can be efficiently supported, production of high-quality Maotai-flavor liquor with the complex ester content reaching 23.00 g / L is assisted, and the method has the advantages of being accurate, efficient and easy to popularize.