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Fingerprint of Polygonum multiflorum and establishment method and application thereof

A technology of fingerprint spectrum and establishment method, which is applied in the application field of fingerprint spectrum in the quality control of Polygonum multiflorum medicinal materials, can solve the problems of not really reflecting the quality of medicinal materials, complex components, etc., and achieves the effect of good reproducibility and strong operability

Inactive Publication Date: 2011-07-06
KANGMEI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For Polygonum multiflorum, due to its complex composition, there are many studies using each single component as an indicator, but a specific component cannot truly reflect the quality of the medicinal material, and there is no unified standard for comprehensively studying its fingerprints to control quality

Method used

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  • Fingerprint of Polygonum multiflorum and establishment method and application thereof
  • Fingerprint of Polygonum multiflorum and establishment method and application thereof
  • Fingerprint of Polygonum multiflorum and establishment method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: Establishment of High Performance Liquid Chromatography for Identification of Radix Polygoni Multiflori

[0047] Instruments and reagents:

[0048] Agilent1100 high-performance liquid chromatography, including online vacuum degasser, binary gradient pump, column thermostat, DAD detector; methanol and phosphoric acid are analytically pure, acetonitrile is chromatographically pure, and water is ultrapure water; 10 batches of Polygonum multiflorum medicinal materials All produced in Deqing, Guangdong, the batch numbers are 040209, 040320, 040426, 040506, 040512, 040705, 040729, 040809, 041018, 050204. It was identified as the dry root tuber of Polygonum.multiflorum.Thynb by the identification teaching and research office of Jiangxi College of Traditional Chinese Medicine. Reference substance: 2,3,5,4-tetrahydroxystilbene-2-o-β-D-glucoside (referred to as stilbene glycoside, 0844-200003), emodin (110756-200110), emodin A Ether (0758-2002006), purchased from C...

Embodiment 2

[0055] Example 2: Methodological investigation of identification of Polygonum multiflorum medicinal material by high performance liquid chromatography

[0056] The following investigations were carried out on the high-performance liquid chromatography for identification of Polygonum multiflorum medicinal material established in Example 1.

[0057] Reproducibility test:

[0058] Get Radix Polygoni Multiflori medicinal material (batch number 050204), prepare 6 samples according to the preparation method of the test sample in Example 1, measure sequentially, investigate the consistency of the relative retention time of chromatographic peaks, peak area ratio. The calculated relative retention time and relative area RSD are both less than 3.0%, the results show that the method has good reproducibility.

[0059] Precision test:

[0060] Take the same part of the test solution (batch number 050204), and continuously inject 6 times to investigate the consistency of the relative re...

Embodiment 3

[0063] Example 3: The relative retention time and relative peak area of ​​the common fingerprint peaks of 10 batches of Polygonum multiflorum medicinal materials and correlation coefficient

[0064] Carry out the sample analysis of 10 batches of Deqing Polygonum multiflorum medicinal materials in the embodiment 1, take No. 4 peak (being stilbene glycoside) as reference peak, the RSD value of the relative retention time of each fingerprint peak is basically all lower than 1.0% (see table 1). But the relative peak area RSD values ​​are larger (see Table 2). It may be due to the difference in harvest time of different batches of medicinal materials and the difference in the content of ingredients.

[0065] Table 1 The relative retention time of the common peaks of 10 batches of Radix Polygoni Multiflori

[0066]

[0067] 040729

0.3420

0.4139

0.6851

1.0000

1.0829

1.9303

2.1259

2.1986

2.4498

2.9065

3.0725

...

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Abstract

The invention discloses a quality control method of Polygonum multiflorum through the fingerprint thereof. The fingerprint is HPLC (high-performance liquid chromatography) fingerprint obtained through HPLC analysis of Polygonum multiflorum. The fingerprint can be used for completely monitoring the quality of Polygonum multiflorum to ensure the stable and uniform quality of Polygonum multiflorum and related pharmaceutical preparations containing Polygonum multiflorum. The invention further discloses the establishment method and the application of the fingerprint of Polygonum multiflorum.

Description

technical field [0001] The invention belongs to the technical field of quality control of traditional Chinese medicines. Specifically, the invention relates to a fingerprint of the Polygonum multiflorum medicinal material and a method for establishing the same, as well as the application of the fingerprint in the quality control of the Polygonum multiflorum medicinal material. Background technique [0002] Polygonum multiflorum is the dry root tuber of Polygonum.multiflorum.Thynb., which is mostly a wild product. Polygonum multiflorum cultivated in Deqing, Guangdong is an authentic medicinal material. It is widely used clinically, mainly for nourishing liver and kidney, benefiting essence and blood, blackening beard and hair, and strengthening bones and muscles. Due to the complexity of traditional Chinese medicine, the traditional method of identifying medicinal materials is still used at present, and this method of relying on experience to monitor the quality of medicinal ...

Claims

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Application Information

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IPC IPC(8): A61K36/704G01N30/02G01N30/06G01N30/36A61P1/14A61P7/06A61K125/00
Inventor 许冬瑾马兴田陈华师薄雯映程文胜
Owner KANGMEI PHARMA
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