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2410 results about "Peak area" patented technology

Chromatographic and mass spectral date analysis

Apparatus, methods, and computer readable media having computer code for calibrating chromatograms to achieve chromatographic peak shape correction, noise filtering, peak detection, retention time determination, baseline correction, and peak area integration. A method for processing a chromatogram, comprises obtaining at least one actual chromatographic peak shape function from one of an internal standard, an external standard, or an analyte represented in the chromatogram; performing chromatographic peak detection using known peak shape functions with regression analysis; reporting regression coefficients from the regression analysis as one of peak area and peak location; and constructing a calibration curve to relate peak area to known concentrations in the chromatogram. A method for constructing an extracted ion chromatogram, comprises calibrating a low resolution mass spectrometer for both mass and peak shape in profile mode; performing mass spectral peak analysis and reporting both mass locations and integrated peak areas; specifying a mass defect window of interest; summing up all detected peaks with mass defects falling within the specified mass defect window to derive summed intensities; and plotting the summed intensities against time to generate a mass defect filtered chromatogram.
Owner:CERNO BIOSCI

Single crystal CVD synthetic diamond material

A single crystal CVD synthetic diamond material comprising: a total as-grown nitrogen concentration equal to or greater than 5 ppm, and a uniform distribution of defects, wherein said uniform distribution of defects is defined by one or more of the following characteristics: (i) the total nitrogen concentration, when mapped by secondary ion mass spectrometry (SIMS) over an area equal to or greater than 50×50 μm using an analysis area of 10 μm or less, possesses a point-to-point variation of less than 30% of an average total nitrogen concentration value, or when mapped by SIMS over an area equal to or greater than 200×200 μm using an analysis area of 60 μm or less, possesses a point-to-point variation of less than 30% of an average total nitrogen concentration value; (ii) an as-grown nitrogen-vacancy defect (NV) concentration equal to or greater than 50 ppb as measured using 77K UV-visible absorption measurements, wherein the nitrogen-vacancy defects are uniformly distributed through the synthetic single crystal CVD diamond material such that, when excited using a 514 nm laser excitation source of spot size equal to or less than 10 μm at room temperature using a 50 mW 46 continuous wave laser, and mapped over an area equal to or greater than 50×50 μm with a data interval less than 10 μm there is a low point-to-point variation wherein the intensity area ratio of nitrogen vacancy photoluminescence peaks between regions of high photoluminescent intensity and regions of low photolominescent intensity is <2× for either the 575 nm photoluminescent peak (NV0) or the 637 nm photoluminescent peak (NV); (iii) a variation in Raman intensity such that, when excited using a 514 nm laser excitation source (resulting in a Raman peak at 552.4 nm) of spot size equal to or less than 10 μm at room temperature using a 50 mW continuous wave laser, and mapped over an area equal to or greater than 50×50 μm with a data interval less than 10 μm, there is a low point-to-point variation wherein the ratio of Raman peak areas between regions of low Raman intensity and high Raman intensity is <1.25×; (iv) an as-grown nitrogen-vacancy defect (NV) concentration equal to or greater than 50 ppb as measured using 77K UV-visible absorption measurements, wherein, when excited using a 514 nm excitation source of spot size equal to or less than 10 μm at 77K using a 50 mW continuous wave laser, gives an intensity at 575 nm corresponding to NV0 greater than 120 times a Raman intensity at 552.4 nm, and/or an intensity at 637 nm corresponding to NV greater than 200 times the Raman intensity at 552.4 nm; (v) a single substitutional nitrogen defect (Ns) concentration equal to or greater than 5 ppm, wherein the single substitutional nitrogen defects are uniformly distributed through the synthetic single crystal CVD diamond material such that by using a 1344 cm−1 infrared absorption feature and sampling an area greater than an area of 0.5 mm2, the variation is lower than 80%, as deduced by dividing the standard deviation by the mean value; (vi) a variation in red luminescence intensity, as defined by a standard deviation divided by a mean value, is less than 15%; (vii) a mean standard deviation in neutral single substitutional nitrogen concentration of less than 80%; and (viii) a colour intensity as measured using a histogram from a microscopy image with a mean gray value of greater than 50, wherein the colour intensity is uniform through the single crystal CVD synthetic diamond material such that the variation in gray colour, as characterised by the gray value standard deviation divided by the gray value mean, is less than 40%.
Owner:ELEMENT SIX LTD

Method for screening traditional Chinese medicine effect related ingredients and model building method

The invention discloses a method for screening traditional Chinese medicine effect related ingredients and a model building method. The method comprises the steps as follows: taking the researched traditional Chinese medicines as objects for preparing multiple sets of sample solutions containing chemical components with different concentrations; selecting part of the chemical components for HPLC (High Performance Liquid Chromatography) analysis, and investigating linear relations between the peak areas and the concentrations; selecting the multiple sets of sample solutions in the linear range, and determining the respective HPLC fingerprint spectrums and the medicine effect indexes of the sample solutions; and obtaining relations between the peak areas and the medicine effect indexes of the ingredients by adopting a linear regression analysis method in the linear relation range, and confirming the actions of the medicine effect related ingredients on the medicine effect indexes according to the positive value and the negative value of the standard regression coefficients and/or the t check results of the ingredients so as to screen out the traditional Chinese medicine effect related ingredients. The method is simple, convenient, easy to implement, reliable and good in commonality, can embody the characteristics of comprehensive actions of the multiple ingredients on the medicine effect indexes, is suitable for screening the traditional Chinese medicine effect related ingredients, and provides a technical data support for discovering and screening traditional Chinese medicine active ingredients.
Owner:SHANDONG UNIV

Method for extracting suaeda salsa flavone and measuring content of flavones in suaeda salsa

The invention relates to a method for extracting suaeda salsa flavone and measuring content of flavone in suaeda salsa. The method comprises the following steps: 1), collecting the suaeda salsa, drying the suaeda salsa and crushing the suaeda salsa; with ethanol as an extractant, soaking the suadeda salsa and carrying out microwave-assisted extracting; filtering; extracting with petroleum ether; transferring the ethanol layer to a macroporous adsorption resin layer; and eluting with water and ethanol aqueous solution sequentially; collecting the eluant, and pressurizing and concentrating the eluant to obtain flavone compound extract; 2), with rutin as a contrast, preparing standard solution with different concentration gradients; measuring the chromatographic peak area at the wavelength of 360nm through a high efficiency liquid chromatography to form flavone content contrast reference; measuring the chromatographic peak area of the to-be-measured solution; obtaining the flavone content of the to-be-measured solution according to the flavone content contrast reference; and obtaining the content of the flavone in the suaeda salsa according to the dilution multiple of the to-be-measured solution. According to the method provided by the invention, soaking and microwave-assisted extracting are adopted to extract flavone, and the content of the flavone is measured by chromatography, so that the method is simple and the solvent is easily available. Besides, the obtained flavone is high in content, and has the physiological and pharmacological activities such as antiviral activity, antioxidant activity, anti-ageing activity and the like.
Owner:YANCHENG INST OF IND TECH
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