90 results about "Peak area" patented technology

The invention relates to the technical field of high performance liquid chromatography detection, in particular to a liquid chromatography determination method and equipment for the content of m-tert-butylphenol, and the method comprises the following steps: determining a to-be-determined mixed solution of the prepared m-tert-butylphenol by using high performance liquid chromatography to obtain chromatographic data; the method comprises the following steps: preparing chromatographic data of standard control group solution samples of m-tert-butylphenol under different concentrations under two preset different wavelengths, and obtaining a nonlinear fitting relationship between the concentrations of m-tert-butylphenol and chromatographic peak areas; analyzing the chromatographic data deviation of the to-be-measured mixed solution, and obtaining the chromatographic peak area of the to-be-measured mixed solution after correction of the intermediate tert-butylphenol; and acquiring the concentration of the intermediate tert-butylphenol in the mixed solution to be measured corresponding to the corrected chromatographic peak area by utilizing a nonlinear fitting relationship. The invention aims to improve the measurement accuracy of the prepared m-tert-butylphenol.

The invention relates to an overlapped chromatographic peak positioning and splitting method and system based on a numerical stable function. The method comprises the following steps: de-noising chromatographic data and correcting a baseline; calculating a second derivative by using SG filtering, grouping continuous points according to a negative threshold value, and determining an initial position of each peak by using a second-order minimum value; establishing a peak function of bidirectional exponential correction by taking a peak position as an initial value, setting parameters and boundaries such as area, center, width, trailing and the like, constructing a residual error minimization target, and performing robust fitting by adopting a trust region reflection algorithm; and indexes such as suitability, output peak area, center, broadening, separation degree, theoretical plate number and the like are verified by residual errors. According to the method, valley point or prior component information is not needed, the method is insensitive to noise and baseline drift, serious overlapping and trailing / leading edge peaks of multiple components can be effectively processed, convergence is high, the method is insensitive to initial values, parameters can be expanded to different peak shapes, and the method is suitable for qualitative and quantitative analysis of automatic and high-flux chromatographic data.

The invention relates to the field of water quality monitoring of a secondary loop of a nuclear power plant, in particular to a synchronous detection system and method for ammonia and hydrazine. The method comprises the following steps: removing impurities in a sample by utilizing filtration and reverse chromatography; the sample is quantified to 240-260 [mu] L; carrying out gradient elution on the sample by using a methanesulfonic acid solution as an eluent; wherein in the first stage, the concentration of a methanesulfonic acid solution is controlled to be 13-18 mmol / L, and a sample is washed; in the second stage, the concentration of the methanesulfonic acid solution is controlled to be 55-70 mmol / L, and the sample is Drawing a low-concentration curve based on the 10-500 [mu] g / L standard solution, and drawing a high-concentration curve based on the 500-3000 [mu] g / L standard solution; and measuring the ionic conductivity values of NH4 < + > and N2H5 < + >, automatically selecting a corresponding concentration curve according to the peak area of the ionic conductivity values, and calculating the concentration of ammonia and hydrazine. According to the invention, the synchronous detection of ammonia and hydrazine concentration is realized, the analysis process is simplified, the use of toxic and harmful substance phenol and the generation of toxic and harmful waste liquid of yellow azo compound are solved, the measurement accuracy is improved, and the relative measurement error can be controlled within + / -2.0%.

The invention discloses a preparation process of a Qingjin phlegm-reducing decoction material standard, an HPLC content determination method and a fingerprint spectrum establishment method aiming at the material standard, under the content determination method, the peak shapes of chromatographic peaks of all components are symmetrical, the separation effect is remarkable, and excellent stability, specificity, linear response, repeatability, precision and accuracy are shown. According to the present invention, the fingerprint characteristics are accurately recorded, the reversed-phase high performance liquid chromatography technology is adopted to successfully obtain 13 common peaks, the baicalin is adopted as the reference peak, the relative retention time and the relative peak area of the common peaks are calculated, and the reference substance comparison is further performed to identify the 8 common peaks; the established fingerprint spectrum method also has the characteristics of good chromatographic peak shape, high separation degree, stable method, excellent repeatability, high precision and the like. In conclusion, the invention provides a set of comprehensive and reliable Qingjin phlegm-reducing decoction material standard quality control reference system, and a scientific basis is provided for the overall quality evaluation of the Qingjin phlegm-reducing decoction.

The invention discloses a radioactive liquid activity detection method, device and equipment, and relates to the field of nuclear energy. The method comprises the following steps: acquiring a to-be-detected water sample, and respectively inputting the to-be-detected water sample into a detector to obtain a detector original pulse signal and a detector fast signal corresponding to the to-be-detected water sample; performing FPGA time window coincidence processing based on the detector original pulse signal and the detector fast signal to obtain a new energy spectrum corresponding to the to-be-detected water sample; obtaining a preprocessed theoretical energy spectrum template, and inputting the theoretical energy spectrum template and the new energy spectrum into the peak decomposition network model to obtain a characteristic peak area list of the target nuclide; and obtaining an efficiency curve of the detector, and obtaining an activity detection result of the target nuclear element in the to-be-detected water sample based on the characteristic peak area list of the target nuclide and the efficiency curve. According to the method, the final result is determined by combining the theoretical energy spectrum template, the original pulse signal of the detector and the fast signal of the detector, so that the spectrum unfolding error of the overlapping peak can be reduced, and the recognition accuracy of the mixed system is effectively improved.

The embodiment of the invention discloses a method and device for testing foam seepage characteristics of a heterogeneous porous medium, electronic equipment and a storage medium, and relates to the technical field of fluid mechanics and nuclear magnetic resonance. The method comprises the steps that a heterogeneous porous medium sample with a clear permeability level difference is prepared, and the permeability level difference of the heterogeneous porous medium sample is determined; setting test parameters according to the permeability level difference of the sample, calibrating imaging parameters of the low-field nuclear magnetic resonance equipment, and setting a weighting mode; performing foam flooding treatment on the sample according to the set injection parameters, acquiring a spectrum of the sample according to the test parameters when foam is injected into a set amount, and acquiring nuclear magnetic imaging of the sample through low-field nuclear magnetic resonance equipment according to imaging parameters and a weighting mode; according to the relaxation characteristic of the spectrum, the main peak endpoint value is used for dividing the pore channel, the foam sweep degree is calculated according to the spectrum peak area of the spectrum, and the foam plugging strength is calculated according to the signal amplitude of nuclear magnetic imaging. According to the method, the problems of poor visualization adaptability and lack of evaluation systems in the prior art are solved.

To provide a photosensitive resin composition and light-shielding film that achieve both high light-shielding properties and reduced reflectivity on both the glass substrate side and the film side, while also being environmentally friendly by using extremely low amounts of fluorine and meeting requirements such as PFAS regulations. [Solution] A photosensitive resin composition characterized by containing components (A) to (F) and satisfying both (i) and (ii) below. (i) When a cured film of the composition is deposited on a glass substrate with an average film thickness in the range of 0.5 to 4.0 μm, and the surface of the film is measured by XPS, the peak area A of SiO2 in the peak fit analysis of the Si2p3 / 2 peak is... SiO2 And, peak area A SiO The ratio (A SiO2 / A SiO The value must be between 0.10 and 10.0. (ii) The ratio of the amount of element F to the total amount of elements Si, C, O, and F present on the surface of the film [F / (Si+C+O+F)] is 5.0% or less.

The invention relates to an underwater gamma-ray energy spectrum repeatability analysis method and device and a storage medium. The method comprises the following steps: acquiring a gamma-ray energy spectrum group measured underwater; performing spectrum unfolding on the gamma-ray energy spectrum group based on a Gold iterative deconvolution spectrum unfolding algorithm to obtain energy of gamma incident rays; solving a peak value and a peak area of the gamma-ray energy spectrum group by adopting a gravity center method; determining a first relative standard deviation corresponding to the peak value and a second relative standard deviation corresponding to the peak area; and determining the repeatability of the gamma-ray spectrum group based on the first relative standard deviation and the second relative standard deviation. According to the method, the background is deducted in a spectrum unfolding mode on the basis of the Gold iteration deconvolution spectrum unfolding algorithm, then the peak value and the peak area are calculated, the background deduction error is reduced, the repeatability is improved, meanwhile, the Gold iteration deconvolution spectrum unfolding algorithm reduces the measurement time, and the measurement accuracy is improved. Therefore, the technical effects of reducing the error of background deduction and improving the repeatability based on the Gold iteration deconvolution spectrum unfolding algorithm are realized.

The invention discloses a method for determining the solution concentration in the crystallization process of 3, 4-dinitrofurazan furoxan oxide, and relates to the technical field of energetic crystal materials. According to the method, an EasySampler and HPLC (High Performance Liquid Chromatography) combined method is utilized, the solubility of DNTF is measured, a standard curve of DNTF solution concentration and peak area is established by utilizing a high performance liquid chromatograph, then the EasySampler is utilized to sample a crystallization process and perform HPLC analysis, and then the solution concentration at each moment is calculated by utilizing an equation of the standard curve. According to the method, EasySampler equal interval sampling is combined with off-line HPLC (High Performance Liquid Chromatography) analysis, and a curve that the concentration of the main component of the solution changes along with the temperature is constructed. In the dominant period of crystal growth, the concentration change rate and the solid-liquid interface area are in an approximately linear relationship. The experimental data provides a necessary demonstration basis for subsequent improvement of a non-ideal system crystallization kinetics theory.

The invention discloses a Toci full-spectrum anion truth value monitoring method, system, equipment and medium, and relates to the technical field of chemical analysis. The method comprises the following steps: acquiring a response signal in a target water sample, drawing a background response peak and a target response peak by taking time as an abscissa axis and the response signal as a ordinate axis, acquiring a peak shape parameter of the background response peak, calculating a difference value between the peak shape parameter and a preset reference peak shape parameter, converting the difference value into a feature correction instruction, and correcting the feature correction instruction according to the difference value. The first peak area of the real response peak is calculated; the initial concentration of target anions is obtained according to the first peak area; the background concentration of background anions is obtained; the concentration adjustment coefficient is calculated according to the background concentration; the target concentration of the target anions is obtained. By implementing the technical scheme provided by the invention, the effect of improving the accuracy of monitoring the target anions is achieved.

Disclosed is a grain-oriented electrical steel sheet in which an insulating coating film does not contain a chromium compound but contains a phosphate. When Gaussian fitting is performed on a 31P-NMR spectrum obtained by measuring the insulating coating film by using a nuclear magnetic resonance method in the range of 16 ppm to - 90 ppm, the ratio of the peak area of the Q1 structure to the total peak area is more than 3% but not more than 18%, and the ratio of the Q1 structure is smaller than the ratio of the Q3 structure.

The invention discloses a high-flux mixed solution quality control method based on relative response factors. According to the method, by introducing an internal standard reference substance and calculating a relative response factor Rr of each target analyte relative to the internal standard, synchronous and rapid calculation and stability dynamic evaluation of all component concentrations in a solution are realized, and the method comprises the following steps: selecting and preparing an internal standard solution; analyzing the mixed solution and the internal standard under the same condition to obtain a chromatographic peak area; calculating Rr; monitoring the change of Rr along with time to judge the component stability; and calculating the current concentration of each component based on the stable Rr value and the interior label information. According to the method, only one or a small number of internal standards are needed to replace a single standard prepared for each component in a traditional method, consumption (about 90%) and workload of standard substances are remarkably reduced, unstable components can be sensitively recognized, and an efficient, economical and reliable quality control solution is provided for high-throughput detection in the fields of environmental monitoring, food safety and the like.

Provided is an electrophotographic photosensitive member that suppresses an image defect resulting from its support and is reduced in long-term potential fluctuation. The electrophotographic photosensitive member is an electrophotographic photosensitive member including a support having a cylindrical shape and a photosensitive layer formed on the support, wherein the support has a surface formed of Al and / or an Al alloy, and wherein the IR peak area ratio A of the surface of the support determined by Fourier transform infrared spectroscopy satisfies the following condition: A≥0.50 where the IR peak area ratio A represents a ratio a / z of a peak area “a” in the range of from 1,016 cm−1 or more to 1,130 cm−1 or less to a peak area “z” in the range of from 650 cm−1 or more to 1,130 cm−1 or less.

The invention relates to a method for measuring the concentration of tetrachloroethylene in water, which comprises the following steps: (1) detecting a standard working solution of tetrachloroethylene by using a high performance liquid chromatograph to obtain a standard working curve of the tetrachloroethylene absorption peak area and absolute concentration of the standard working solution of tetrachloroethylene; (2) detecting a water sample to be detected by using the high performance liquid chromatograph to obtain the tetrachloroethylene absorption peak area of the water sample to be detected; a mobile phase used in the detection of the high performance liquid chromatograph comprises an organic phase and ultrapure water, and the volume ratio of the organic phase to the ultrapure water is (70-90): (30-10); the detection wavelength of the detection is 212 to 216 nm; the temperature of a column oven of the high performance liquid chromatograph is 35-40 DEG C. According to the method disclosed by the invention, the peak appearance time is short, the separation effect of the detected main peak and the impurity peak is good, the fitting degree of the obtained standard working curve is high, the separation degree of the main peak and the impurity peak in the detection of an actual water sample containing tetrachloroethylene is high, and the detection result is reliable.

[Problem] To provide a workpiece processing film and a workpiece processing method with which it is possible to hold a workpiece while keeping an attachment surface clean by being attached to the workpiece during workpiece processing. [Solution] This workpiece processing film is used for processing a workpiece, wherein: the workpiece processing film is a substrate-less double-sided adhesive film; and when 10 μL of a toluene solvent, obtained by immersing a workpiece processing film having a size of 80 mm×100 mm in 15 g of toluene at 25ºC for 24 hours, is measured by gel permeation chromatography, and the measurement results are shown as a chromatogram in which the vertical axis is a detection voltage and the horizontal axis is an elution time from the introduction of the toluene solvent to the elution from a column, the peak area of a detection component from an elution time corresponding to a time when the number-average molecular weight in terms of standard polystyrene is 2,500,000 to an elution time corresponding to a time when the number-average molecular weight in terms of standard polystyrene is 200 is at most 4,000 mV∙sec.

The present invention discloses a method for constructing a characteristic spectrum of Trillium gracile for distinguishing Trillium gracile and its processed products and counterfeits, and its application. By optimizing column temperature and flow rate, optimizing chromatographic columns and other conditions, the present invention allows the Trillium gracile characteristic spectrum to contain more information, with a more stable baseline and better chromatographic peak separation. Through multi-batch testing, common peaks are selected and identified using reference substances. On this basis, Trillium gracile and Trillium gracile can be quickly distinguished through different chromatographic peaks. Simultaneously, through mathematical and statistical analysis, characteristic peaks that can distinguish Trillium gracile from vinegar Trillium gracile slices are optimized, and relative peak areas are optimized, providing a rapid and information-rich characteristic spectrum construction method for quality control analysis of distinguishing Trillium gracile from vinegar Trillium gracile slices.

The application discloses a method for drawing the dissolution curve of perphenazine tablets by high performance liquid chromatography, comprising the following steps: adding perphenazine tablets and preheated dissolution medium into a plurality of dissolution cups in sequence under the light-proof environment; using a dissolution instrument to obtain dissolution liquid by sampling the plurality of dissolution cups for multiple times, 5mL of the dissolution liquid is sampled each time and the isothermal dissolution medium is supplemented at the same time; filtering the dissolution liquid, taking the filtered liquid to obtain the test sample solution, and storing the test sample solution for use in the dark; preparing the control sample solution with the perphenazine tablet concentration of 2.012ug / mL and 4.024ug / mL respectively, and storing the control sample solution for use in the dark; using a high performance liquid chromatograph, sucking the test sample solution and the control sample solution into the high performance liquid chromatograph according to preset chromatographic conditions; recording the peak area, and calculating the dissolution amount at each time point according to the formula; taking the dissolution time as the horizontal coordinate and the accumulated dissolution amount at each time as the vertical coordinate, and drawing the dissolution curve.

The invention belongs to the technical field of analysis and detection, and particularly relates to a sulfur hexafluoride isotope abundance detection method. The sulfur hexafluoride isotope abundance detection method provided by the invention comprises the following steps: detecting a to-be-detected sample by using a gas chromatograph-mass spectrometer to obtain a total ion flow chromatogram of sulfur hexafluoride; the gas chromatography test conditions are as follows: a DB-5MS capillary column is adopted, the split ratio is (20-80): 1, and the sample size is 1-10 L; the mass spectrum test conditions comprise that the voltage of a multiplier is + 300 to + 600, and the half-peak width is less than 0.6; and carrying out < 32 > SF < 5 + >, < 33 > SF < 5 + > and < 34 > SF < 5 + > ion extraction on the total ion chromatogram of sulfur hexafluoride to respectively obtain the peak area of < 32 > SF < 5 + >, the peak area of < 33 > SF < 5 + > and the peak area of < 34 > SF < 5 + >, and calculating the abundance of < 32 > SF < 5 + >, the abundance of < 33 > SF < 5 + > and the abundance of < 34 > SF < 5 + >. The detection method provided by the invention has the advantages of economical efficiency, high analysis speed and high accuracy.

This polythiol composition comprises a compound (A) represented by formula (1) and a compound (B) represented by formula (2). In high-performance liquid chromatography measurement performed using a prescribed detector, the peak area of the compound (B) is 9.0 area% or less with respect to 100 area% of the peak area of the compound (A). (In formula (1), the plurality of R's are identical or different from each other, and each represent a divalent hydrocarbon group having 1-6 carbon atoms.) (In formula (2), the plurality of R's are identical or different from each other, and each represent a divalent hydrocarbon group having 1-6 carbon atoms. a1, b1, c1, d1, e1, f1, g1, and h1 are identical or different from each other, each represent an integer of 0-3, and the sum of the same is 6.)

The invention relates to the field of substance purity analysis methods, and discloses a method for analyzing the purity of o-phthalic anhydride by using gas chromatography, which comprises the following steps: firstly, preparing a standard solution of which the concentration of each component is close to that of each component in crude phthalic anhydride to be analyzed, and analyzing the standard solution for more than three times; calculating a correction factor of each substance after the peak area repeatability error of each substance is less than 3%; and inputting the correction factor into a method-component table, comparing the spectrogram of the to-be-detected sample solution with the spectrogram of the standard substance solution, determining peak localization of corresponding substances in the to-be-detected sample solution according to retention time, and calculating the purity of phthalic anhydride in the to-be-detected sample by adopting an area correction normalization method. The method is simple to operate, short in analysis time and good in stability and reproducibility.

The invention discloses a method for detecting dibenzoyl thiamine disulfide and related substances thereof. The detection method comprises the following steps: preparing dibenzoyl thiamine disulfide reference substance solutions with different concentrations, recording corresponding chromatograms and corresponding peak areas by adopting a high performance liquid chromatograph, and drawing a dibenzoyl thiamine disulfide standard curve; respectively drawing standard curves of the related substances in the same way; the method comprises the following steps: injecting a to-be-detected sample solution into a high performance liquid chromatograph, recording chromatograms under the wavelengths of 233 nm and 280 nm, and determining the nature of a main peak and impurity peaks by comparing the retention time of each peak in the to-be-detected sample solution, a p-dibenzoylthiamine disulfide reference substance solution and a related substance solution. The invention provides a high performance liquid chromatography which is used for analyzing related substances in diphenylthiamine, and the method is good in specificity, high in sensitivity, low in detection limit, simple to operate, efficient in separation and capable of being used for realizing quality control of the compound.

Pentacyclopentadecandimethanol product in which the area ratio of the sum of the areas of a peak group occurring in the range of the relative retention time of 0.70 to 0.95 to the total peak area is 1.00% or less in a liquid chromatographic analysis under the following conditions, when the relative retention time of the peak of pentacyclopentadecandimethanol is determined to be 1.00: (Conditions for liquid chromatographic analysis) Analysis columns: two columns (mean particle size 3 µm, mean pore size 1.5 nm, length 15 cm × inner diameter 6.0 mm), whose base material is styrenedivinylbenzene, coupled with one column (mean particle size 3 µm, medium pore size 2.0 nm, length 15 cm × inner diameter 6.0 mm), whose base material is styrenedivinylbenzene, are used. Sample concentration: 5 wt% (dilution solvent tetrahydrofuran) Column oven temperature: 40°C Carrier: Tetrahydrofuran Column liquid flow rate: 0.35 ml / min Detector: Refractive index detector (cell temperature: 40°C)

The application provides a testing method for carbon coating completeness of a silicon-based material, and belongs to the technical field of material testing. The method comprises the following steps: placing a first mass of strong alkaline material and a second mass of testing silicon-based material in a reaction tank to perform a reaction, and obtaining first current information output by a detection device; placing the first mass of strong alkaline material and a contrast material in the reaction tank to perform a reaction, and obtaining contrast current information output by the detection device; drawing a test image based on the first current information and the contrast current information; and calculating the carbon coating completeness of the testing silicon-based material based on the peak area and the peak current value of the testing silicon-based material in the test image, the peak area and the peak current value of the contrast material, and the second mass and the mass of the contrast material. The testing method for carbon coating completeness of the silicon-based material provided by the application can simply and conveniently test the carbon coating completeness of the silicon-based material.

The invention belongs to the field of chemistry, and relates to a method and a system for rapidly detecting TiC in carbide slag. The method comprises the following steps: preparing a standard sample: preparing a series of carbide slag standard samples with known TiC content; acquiring XRD (X-Ray Diffraction) data: scanning the standard sample and the sample to be detected by using an X-ray diffractometer in a range of 2theta = 34-38 degrees to obtain a diffraction pattern; processing data and drawing a standard working curve: extracting a characteristic diffraction peak of TiC at 2theta = 36.1 + / -0.2 degrees, carrying out area integration, and establishing a standard working curve of the TiC content and a peak area integral value; and determining an unknown sample: carrying out the same treatment on the sample to be detected, substituting the peak area integral value into the standard working curve, and calculating to obtain the TiC content. According to the method, a diffraction peak with a diffraction angle 2theta of 36.1 degrees is adopted for fitting and then quantitative analysis is performed, so that the problem of long consumed time of an existing method for measuring the content of TiC in the carbide slag is solved.

A method for determining a dilution factor of a sample comprising an analyte (115) of interest is disclosed. The method comprises the following steps:a) (136) preparing a mixture (113) by diluting defined volumes of calibrators (114) comprising the analyte (115) of interest with a defined volume of a dilution reagent comprising at least one first internal standard ISTDa (118);b) (138) performing at least one sample preparation workflow (120) on a defined volume of the mixture (113) thereby generating a prepared mixture (122), wherein the sample preparation workflow (120) comprises adding at least one second internal standard ISTDb (124);c) (140) performing at least one sample measurement on the prepared mixture (122) comprising determining a peak area for each of the first internal standard ISTDa (118) and the second internal standard ISTDb (124) using an in vitro diagnostic medical device (110) and determining an area ratio ARsample of ISTDa (118) and ISTDb (124) by using at least one processing device (130);d) (142) performing at least one calibration measurement on a plurality of calibration samples (128), wherein the calibration samples (128) have a predefined concentration of ISTDa (118) and ISTDb (124), and determining an averaged area ratio ARcalibration,av of ISTDa (118) and ISTDb (124) of the calibration samples (128) by using the processing device (130);e) (144) determining the dilution factor by determining a deviation of the area ratio ARsample and the averaged area ratio ARcalibration,av by using the processing device (130).

An automatic detection method for a preparative chromatograph, comprising the following steps: preparing a standard chromatogram; injecting a sample that matches the standard chromatogram, and the chromatograph outputs a chromatogram to be detected; setting the chromatographic peak list of the chromatogram to be detected to be displayed in descending order of peak area; selecting the first n chromatographic peaks of the chromatogram to be detected and the first n chromatographic peaks of the standard chromatogram, comparing whether the retention time of the first chromatographic peak of the chromatogram to be detected is close to the retention time of the first chromatographic peak of the standard chromatogram, and judging whether the retention time of this chromatographic peak of the chromatogram to be detected is within the collection range, obtaining a corresponding score, and the collection range is set in advance according to experience; continuing to complete the comparison of the other n-1 chromatographic peaks according to the same scoring rule; adding up the scores, and if the percentage obtained by dividing by the total score is within the set threshold, it is considered that the two chromatograms are similar and the separation can continue, otherwise it is prompted to stop the separation.

The present application relates to a kind of chromatographic data optimization processing method of gas chromatograph, belong to data analysis technical field, comprising: based on the detection data of septum, determine the aging state grade of septum;Based on the aging state grade of septum and the material of septum, determine the release material of septum;Obtain the chromatographic data of sample analysis by gas chromatograph, remove the interference peak corresponding to the release material of septum from chromatographic data, generate first chromatographic optimization data;Based on first chromatographic optimization data and the aging state grade of septum, determine sample material loss compensation numerical value, and optimize chromatographic peak area value in first chromatographic optimization data, generate second chromatographic optimization data.The method is based on septum aging prediction, interference peak removal and quantitative correction linkage, both solve the problem of peak shape pollution, and also solve the problem of quantitative inaccuracy caused by aging, and then the reliability of gas chromatographic data and the accuracy of analysis result can be improved.

The invention relates to the technical field of medicines, in particular to a process quality control method of semeglutide, which is characterized in that a compound 1 has serious solution instability and can be quickly degraded along with time, and in a subsequent synthesis process, it must be ensured that an intermediate which is prepared freshly or is confirmed to be qualified in purity is used, and high performance liquid chromatography process monitoring is introduced. The invention provides a high performance liquid chromatography real-time monitoring method matched with a compound 1 to accurately control the reaction process so as to effectively inhibit an impurity chain reaction caused by degradation of raw materials, and for the characteristics of instability and continuous decomposition of the compound 1 in a solution, the invention provides a high performance liquid chromatography real-time monitoring method matched with the compound 1. The raw material conversion rate and the generation conditions of key over-modified impurities 1 and 2 can be synchronously monitored in the reaction process, and the raw material peak area smaller than or equal to 2.0% and the specific impurity peak area smaller than or equal to 5.0% (or 2.0%) serve as scientific and accurate reaction termination criteria. According to the active process control strategy, accumulation of impurities is inhibited from the source, and the problems of inaccurate feeding and batch difference caused by degradation of intermediates are solved.

The invention discloses a histidine content detection method, and relates to the technical field of detection, and the histidine content detection method comprises the following steps: S1, preparing a standard curve solution, a verification standard solution, a test solution and a blank control solution; s2, hydrophilic chromatography taking thiobetaine as a bonding phase is taken as a chromatographic column, gradient elution is carried out on a mobile phase A and a mobile phase B, the mobile phase A is an acetonitrile solution, the mobile phase B is 0.3% formic acid + 20mM ammonium formate solution, and a CAD detector is adopted to detect each sample; and S3, according to an external standard method, calculating a linear regression equation by taking a peak area logarithm value of the standard curve solution as a vertical coordinate y and a histidine concentration logarithm value of the standard curve solution as a horizontal coordinate x, and then calculating the histidine content in the test solution. The method is low in operation difficulty, high in accuracy, good in repeatability and short in consumed time.

The present invention provides a method for detecting the relative concentration of microscopic optical flaws in micro-areas of the processed surface of a fused quartz optical element, which belongs to the field of engineering optics technology. In order to solve the problem that the existing technology for characterizing point defects on the processed surface of a fused quartz element is only suitable for characterizing and distinguishing the types of point defects, and there is no effective method for detecting the relative concentration of point defects. The present invention conducts a photoluminescence detection experiment on the micro-defect area of ​​the processed surface of a fused quartz optical element to obtain the point defect type in the defect area and the peak area of ​​the sub-peak curve corresponding to different point defects, establishes the relationship between the concentration of lone pair electrons contained in the point defect and the peak area of ​​the sub-peak curve, calculates the relative concentration of lone pair electrons contained in different point defects, and calculates the relative concentration of different point defects in the micro-defect area of ​​the processed surface of the fused quartz in combination with the chemical structure and reaction law of the point defect. The present invention fills the technical gap that is currently unable to obtain the relative concentration of point defects on the surface of a material.