82results about How to "Reliable method" patented technology

The invention discloses a power meter reading concentrator remote upgrading system and method based on a 4G communication module. The system comprises a power meter reading concentrator, a remote server, a management terminal and a security authentication module. The concentrator is internally provided with a 4G communication module and establishes secure communication with a remote server through a 4G network, so that remote upgrade of firmware or software is realized. The system adopts block transmission and breakpoint resume technologies, and it is ensured that the upgrade package can still be efficiently and completely transmitted under the condition that the network is unstable. In the upgrading process, SSL / TLS encryption is adopted for data transmission, and safety is guaranteed; meanwhile, the system has self-checking and rollback functions, and it is ensured that the system automatically recovers to a safe state when upgrading fails. According to the invention, the upgrading and maintenance efficiency of the electric power meter reading system is remarkably improved, the operation and maintenance cost is reduced, and the reliability and safety of the system are improved.

The invention provides a method for identifying gallnut honey by utilizing a characteristic marker, and belongs to the technical field of honey detection. According to the method, kynurenic acid is taken as a characteristic marker, quantitative detection is carried out on kynurenic acid through a high performance liquid chromatography-diode array detector method, and the content of kynurenic acid in gallnut honey is not lower than 14 mg / kg. The kynurenic acid is used as the characteristic marker of the gallnut honey for the first time, the developed identification method also can successfully discriminate the gallnut honey, and the method has the advantages of simple pretreatment, common instruments and mobile phases, low cost, short time, accuracy and the like, and is suitable for industrial production. The method has application potential in gallnut honey identification and product grading.

The invention relates to the technical field of raw silk inspection, in particular to a method for simultaneously identifying feed silkworm rearing raw silk, mulberry leaf silkworm rearing raw silk and fresh cocoon raw silk / dry cocoon raw silk, which comprises the following steps: grinding the feed silkworm rearing raw silk, mulberry leaf silkworm rearing raw silk, fresh cocoon raw silk and dry cocoon raw silk from the same source, adding equal amount of hydrochloric acid after grinding, mixing and reacting, stirring by using a glass rod, and standing for 24 hours; then carrying out ultrasonic treatment and centrifugation, and removing supernate; removing the supernatant, adding the same amount of deionized water, stirring, washing, standing, pouring out the supernatant, repeating for several times, centrifuging, taking the precipitate, drying, grinding and crushing to obtain an extract; and determining the obtained extract by adopting a stable isotope ratio mass spectrometer, and judging the silkworm rearing raw silk of the feed, the silkworm rearing raw silk of the mulberry leaves, the fresh cocoon raw silk and the dried cocoon raw silk according to the N content and the 15N relative content. The method for detecting the total N content and the < 15 > N relative content in the raw silk is reliable, high in recognition rate, visual and reliable in experimental result and high in accuracy.

The invention provides a weak supervision defect detection method for a component image, and belongs to the field of digital image processing and industrial detection. According to the method, the significant characteristics of an attention mechanism are considered, the advantage that the U-Net + + can achieve high-precision pixel-level image segmentation under a small sample is utilized, a triple convolution attention module and a self-supervision convolution attention module are introduced into a U-Net + + network for structure optimization, and a weak supervision mode training model is provided. The method is suitable for component image defect detection tasks under the conditions that the number of normal samples is large and the number of defect samples is small, the method is reliable and good in stability, the problems existing in a supervised method and an unsupervised method can be effectively solved, the component image covers a surface image for detecting surface defects and a CT image for detecting internal defects, and the detection accuracy is high. The method can be applied to high-precision internal and external defect detection of components.

The device comprises a water level control module and a grouting backfilling module, the water level control module comprises a flange pipe, a sealing end, a drainage valve, an air compression valve, an air pressure servo pump and a laser range finder, and the sealing end is in sealed connection with a depressurization well pipe through the flange pipe; the sealing end is respectively connected with the drainage valve and the compressed air valve, and a laser range finder is arranged at the top of the sealing end; the grouting and backfilling module comprises a grouting pipe, a grouting pump, a grouting valve, a grouting material, backfilling granules and concrete; the grouting pipe penetrates through the top of the sealing end and is connected with the grouting valve and the grouting pump; the backfill aggregates are laid at the bottom of the depressurization well pipe, the grouting material is injected into the depressurization well pipe through the grouting pipe, the liquid level is higher than the top surface of the backfill aggregates, and the concrete is poured above the grouting material. The underground water level is controlled by pressing air into the pressure reduction well pipe, the influence of underground water flowing on the grouting effect is avoided, and the grouting construction quality and the sealing effect are guaranteed.

The present invention relates to the technical field of drug analysis, and is used to solve the problem that the existing quality method of pazufloxacin mesylate cannot separate photodegradable impurities, and provides a quality control method for pazufloxacin mesylate injection, comprising the following steps: (1) preparing a test solution; preparing a control solution; preparing a reference solution; (2) recording a chromatogram of the reference solution and the reference solution; (3) recording the chromatograms of the test solution and the reference solution; (4) calculating the content of pazufloxacin mesylate by peak area according to the external standard method. The present invention adjusts the ratio of two mobile phases in the gradient according to the polarity of each impurity, and changes isocratic elution to gradient elution, so that each impurity with different polarities can be well separated.

The embodiment of the present invention relates to the field of biometric identification technology, and discloses a method, cloud platform and system for rapid screening of drug addicts based on skin spectra. The method includes: using a skin spectrum information collector to collect spectrum sequence data of the non-dominant palm of the screened person; preprocessing the spectrum sequence data; based on the gender of the screened person, inputting the preprocessed spectrum sequence data into different drug abuse recognition models for identification to obtain drug abuse screening results; and feeding back the drug abuse screening results to a mobile terminal for display. The scheme for rapid screening of drug addicts based on skin spectra provided by the embodiment of the present invention has the characteristics of in-situ non-destructiveness compared to traditional sampling and detection methods (blood tests, urine tests, hair tests, etc.), and has the characteristics of rapidity and less restrictions compared to non-invasive screening methods (breath tests, iris / pupil tests). In terms of rapid on-site screening, the method of the present invention is more effective, simple, reliable, and has a higher accuracy rate.

The invention discloses a molecular marker C153 related to growth traits of portunus trituberculatus and application of the molecular marker C153. The nucleotide sequence of the molecular marker C153 is as shown in SEQ ID NO. 1, and the sequences of primers for detecting the molecular marker C153 are as shown in SEQ ID NO. 2 and SEQ ID NO. 3. The molecular marker C153 is an SNP (Single Nucleotide Polymorphism) marker, and the growth speed of an individual with the 115th genotype of the molecular marker C153 being GG is obviously higher than that of a portunus trituberculatus individual with genotypes of AG and AA. The molecular marker C153 provided by the invention can be used for accelerating the breeding speed of the growth traits of the portunus trituberculatus, saving a large amount of manpower and material resources, promoting the process of breeding improved varieties of the portunus trituberculatus, being beneficial to the healthy and sustainable development of the portunus trituberculatus industry and having a wide market application prospect.

The invention discloses a large-scale wind turbine generator wind energy tracking control time history optimization method considering an aging effect. The method comprises the following steps: S100, quantitatively evaluating unit performance degradation, performing SCADA (Supervisory Control and Data Acquisition) parameter selection and data preprocessing, constructing a unit performance degradation quantitative evaluation model, and evaluating the aging state of a unit through field SCADA data; s300, optimizing the control time history of the unit, and judging the deterioration state of the unit through SCADA (Supervisory Control and Data Acquisition) data; and S200, maximum wind energy tracking control is carried out, a blade tip speed ratio-wind energy utilization coefficient curve under the aging state of the unit is drawn according to the evaluated aging state of the unit, the basic performance of the wind turbine generator is approximately described, and the maximum value of the wind energy utilization coefficient is obtained. The optimization method is provided for solving the problem that the wind power utilization coefficient of the wind turbine generator is reduced due to natural aging of the wind turbine generator, so that the power output of the wind turbine generator is directly influenced, the optimal operation condition parameters of the wind turbine generator in the aging state can be more accurately obtained, the operation condition is optimized, and the operation power generation efficiency is improved.

The invention discloses a guided rocket inner ballistic proportioning parameter design method for reducing peak heat flow, which puts forward design requirements on guided rocket engine inner ballistic parameters from the perspective of overall design, introduces inner ballistic curve proportioning parameters, and realizes reduction of outer ballistic peak heat flow by optimizing the proportioning parameters. The matching parameters are iterated through a double-loop optimization framework, the outer loop aims at reducing peak heat flow, the inner loop is rapidly evaluated through a ballistic optimization method, and the optimal matching parameters of the inner ballistic of the guided rocket engine are rapidly obtained. According to the method, integrated design of the inner trajectory and the outer trajectory of the guided rocket is achieved, the design dimension of the guided rocket is increased, a new scheme optimization approach is provided for overall designers, and the adaptation degree of an overall scheme and an engine scheme is improved.

The invention provides an elevator sterilizing, ventilating and air-conditioning system and an operation method thereof. The system comprises a power unit, a semiconductor air-conditioning unit, a sterilizing unit, a ventilating unit, a monitoring unit and a control display unit. According to the system, wind power generation is provided through elevator shaft piston wind and the chimney effect during elevator running, cold and heat supply is provided for air in an elevator shaft through the semiconductor refrigerating and heating technology, and meanwhile an elevator car sterilizing, ventilating and filtering device is provided. The invention further provides a method of the sterilizing, ventilating and air-conditioning system for the elevator, normal and efficient work of the system can be guaranteed, multiple functions of ventilating, sterilizing, refrigerating, heating and air conditioning are integrated, meanwhile, the running resistance of the elevator is effectively reduced, and the chimney effect in an elevator shaft can be effectively relieved. In addition, the user can sense real-time air quality data and the number of users of the elevator, the use requirements of the user can be conveniently adjusted, and the high engineering practice value is achieved.

The invention discloses a bridge section steel expansion joint maintenance construction method based on a novel formwork system, and particularly relates to the technical field of bridge section steel expansion joint maintenance construction, and the method comprises the following steps: S1, preparation before construction, S2, on-site construction, and S3, construction summarization. When a traditional profile steel expansion joint is maintained and constructed, (1) flexible materials such as foam plates and cloth which are low in strength and not easy to fix are adopted to fill the joint and support concrete, the concrete in the expansion joint is easy to expand, leak slurry, block and pollute a lower bridge structure, and the working performance of free expansion of the structure is influenced; and (2) the gaps and the retaining concrete are filled with battens or square timbers, so that the installation difficulty is high, the formwork is easy to deviate, and the difficulty of taking out the gap filling material in the later period is high. According to the method, the maintenance construction method that a traditional joint filling material is replaced with the rail inner supporting type aluminum formwork system is adopted, operation is easy, the construction speed is high, precision is high, the side face structure size of the post-cast strip joint can be accurately controlled, and the phenomena of concrete formwork expansion and slurry leakage are eliminated; by changing the specifications of the self-sealing turn buckles, the formwork system can be suitable for construction of various types of profile steel expansion joint structures; meanwhile, in the construction process, through combination of the formwork and the horizontal laser instrument, a traditional rough construction method for controlling the profile steel and concrete surface elevation and flatness is changed, and the overall maintenance construction quality of the profile steel expansion joint structure is improved.

The embodiment of the invention relates to the technical field of biological recognition, and discloses a method, a cloud platform and a system for rapidly screening drug addicts based on a skin spectrum. The method comprises the following steps: acquiring spectrum sequence data of a non-habitual palm of a screened person by adopting a skin spectrum information acquisition instrument; preprocessing the spectrum sequence data; based on the gender of the screened person, inputting the preprocessed spectrum sequence data into different drug taking identification models for identification, and obtaining a drug taking screening result; and feeding back a drug taking screening result to the mobile terminal for displaying. According to the scheme for rapidly screening the drug addicts based on the skin spectrum, compared with a traditional sampling detection method (blood detection, urinalysis, hair detection and the like), the scheme has the advantages of being free of damage in situ, and compared with a non-invasive screening method (expiration detection and iris / pupil detection), the scheme has the advantages of being rapid and few in limitation, and the scheme can be used for rapidly screening the drug addicts on site. The method is more effective, simpler, more reliable and higher in accuracy.

The invention discloses a method for inhibiting laser processing damage of a heterogeneous material, which comprises the following steps: a processed material is immersed in an auxiliary liquid, so that the processed part of the processed material is fully infiltrated by the auxiliary liquid, the processed material is a solid heterogeneous material, and under the central wavelength of used laser, the room temperature complex refractive index of the processed material is n1, the effective complex refractive index of the auxiliary liquid at the working temperature is n2, and n1 and n2 meet matching conditions; and the machined material is subjected to laser subtractive machining, and the residual auxiliary liquid is removed after machining is completed. By selecting proper auxiliary liquid and applying optical parameters matched with the auxiliary liquid, binary interaction of the laser and the heterogeneous material is converted into interaction of the laser, the heterogeneous material and the auxiliary liquid, so that comprehensive effects of stray light suppression, micro-stripping effect of a thermal damage area caused by laser-induced cavitation, suppression of a heat affected area by liquid cooling and the like are achieved; and the processing quality of the heterogeneous material is obviously improved.

The invention discloses a thermomechanical treatment method for improving the obdurability and the plate shape of an Al-Cu-Mg alloy plate. The thermomechanical treatment method comprises the following steps: (1) carrying out pre-stretching treatment on a deformed plate; (2) carrying out single-stage or graded solid solution heat treatment on the pre-stretched plate, wherein the final solid solution heat treatment temperature is T1; (3) cooling the plate subjected to solid solution treatment to a temperature T2 at an average cooling rate V1, and then cooling the plate from T2 to a room temperature at an average cooling rate V2; (4) the quenched plate is subjected to cold deformation treatment after being placed at the room temperature; and (5) the plate subjected to cold deformation treatment is subjected to pre-stretching treatment, and then natural aging is conducted under the room temperature condition. According to the method, the Al-Cu-Mg alloy plate is subjected to pre-stretching, solid solution heat preservation, graded controlled cooling quenching, room temperature standing, cold deformation, pre-stretching and natural aging treatment, and the Al-Cu-Mg alloy can obtain an ideal obdurability matching relation on the premise of controlling residual stress and the plate shape.

The application discloses a portrait inspection and identification method based on gait cycle decomposition and multi-phase force analysis, and comprises the following steps: extracting the skeleton key points of a target object in a target object image set in a comparison video and the confidence score of each skeleton key point; extracting the features of the target object in the target object image set according to the skeleton key points of the target object, and obtaining the target object features, wherein the target object features comprise basic features, gait cycle features, gait stage features and gait parameter features; integrating the basic features, the gait cycle features and the gait stage features to obtain comprehensive features; respectively calculating the similarity of the target object features and the comprehensive features in the comparison video to obtain similarity calculation results and overall similarity calculation results; and obtaining the identity identification result of the target object according to the similarity calculation results and the overall similarity calculation results.

This invention belongs to the field of battery materials technology, and discloses a single-crystal lithium nickel manganese oxide material, its preparation method, and a lithium-ion battery. The single-crystal lithium nickel manganese oxide material contains Li... x Ni 2‑x The percentage content of O2 is C≤1.6%, C=(I LixNi2‑xO2 / (I LixNi2‑xO2 +I LiNi0.5Mn1.5O4 ))×100%, I LixNi2‑xO2 For Li in the XRD refinement results x Ni 2‑x The diffraction peak intensity corresponding to O2, I LixNi2‑xO2 +I LiNi0.5Mn1.5O4 To add LiNi to the XRD refinement results 0.5 Mn 1.5 O4 and Li x Ni 2‑x The diffraction peak intensity of the O2 two-phase model indicates a grain size of 5–11 μm. The aforementioned single-crystal lithium nickel manganese oxide material was prepared via a lithium-deficient high-temperature sintering and lithium-replenished low-temperature sintering process. Li x Ni 2‑x Large-particle monocrystalline lithium nickel manganese oxide materials with low O2 content avoid electrolyte decomposition under high voltage, thus preventing the generation of HF and Li. x Ni 2‑x The O2 reaction can effectively improve the cycle stability of the battery. Simultaneously, the high compaction density of monocrystalline lithium nickel manganese oxide material can enhance the battery's energy density, thermal stability, mechanical properties, and durability. The method for preparing monocrystalline lithium nickel manganese oxide material in this invention is relatively simple, reliable, and effective, and can utilize existing production lines.

The invention relates to a method for a motor vehicle (100) to select a preferred lane (230) for entering a toll zone (200) comprising a plurality of lanes (210, 220), the method comprising the following steps: a) detecting the lanes of the toll zone, b) determining, for at least some of the lanes, first data (d) relating to the distance between the motor vehicle and the respective lane ij ), and second data related to the number of other vehicles located on the corresponding lane; c) minimizing a cost function that depends on each first data and each second data to identify the preferred lane; and d) determining the possibility or risk of the motor vehicle changing lanes to move towards the preferred lane.

The application relates to the technical field of Scylla paramamosain, in particular to a method for editing an eyes absent-2 gene of Scylla paramamosain and application. The application synthesizes sgRNA as shown in SEQ ID NO:1 which is targeted to the eyes absent-2 gene, then combines the sgRNA with a Cas9 protein to obtain an RNP compound, the RNP compound is introduced into a fertilized egg of a single cell stage of Scylla paramamosain in a manner of microinjection, and Scylla paramamosain F0 generation with a target gene mutation can be obtained after hatching. The application efficiently realizes editing of the gene of Scylla paramamosain, and provides an effective technical means for carrying out breeding and gene function research of Scylla paramamosain.

The present invention discloses an eccDNA marker related to the weight of Hu sheep and its detection primer and application. The eccDNA marker refers to the extrachromosomal circular DNA produced in the Hu sheep genome region chr19:22919317-22919972. The Hu sheep genome DNA is used as a template, and a "back-to-back" primer is used to amplify a partial DNA fragment containing an eccNDA joint by PCR, and the PCR product amplified by the "head-to-head" primer is used as a control. According to the agarose gel electrophoresis results, the Hu sheep individuals are divided into an increase type or a deletion type, wherein the increase type of Hu sheep weight is significantly higher than the deletion type. The present invention detects an eccDNA molecular marker closely related to the weight of Hu sheep at the DNA level, and is applied to the marker-assisted selection of Hu sheep to accelerate the genetic breeding process of Hu sheep.

The method for monitoring the resonant capacitor state of the medium-voltage direct-current LLC resonant converter comprises the following steps: closing the switch tube S 11 ~S 41 of the full-bridge at the initial moment when the LLC resonant converter is stopped, then turning on the switch tube S 31 and S 41 after the closing process lasts for 500 ns, forming a discharge loop of a resonant cavity independent of the latter stage, then collecting the time sequence data of the resonant current in the resonant cavity, and estimating the resonant capacitor by using a nonlinear parameter identification algorithm, wherein the estimation is sequentially performed by encoding, initializing the population, calculating the individual fitness, selecting the cross variation evolution, and iterative optimization, finally searching for the optimal resonant current estimation value, and calculating the resonant capacitor estimation value based on the corresponding resonant frequency and resonant inductance. The present application estimates the resonant capacitor value based on the discharge curve of the limited sampling data, has low cost and high reliability, and the present application estimates the parameters by using the nonlinear parameter identification algorithm, and has high estimation accuracy.

The invention discloses a momentum wheel mounting structure optimization and verification method suitable for in-orbit vibration source frequency avoidance, and the method comprises the steps: determining whether the momentum wheel generates resonance at a normal working rotating speed or not through the response measurement of the speed increasing process of the momentum wheel under the condition of a free boundary of a whole satellite, and further generating disturbance vibration to other sensors; establishing a system-level whole satellite model containing an assembly or a local simulation model capable of reflecting installation local rigidity, and carrying out modal analysis; the method comprises the following steps: under limited space constraints, carrying out operation efficacy and reinforcement state simulation iteration without disassembling a product, determining a reinforcement mode direction according to a formation, determining specific parameters according to frequency, and carrying out convergence to obtain a lightest, most efficient and operable integrated lightweight reinforcement structure under different momentum wheel mounting positions; and performing reinforcement design to the whole satellite, performing momentum wheel acceleration process response measurement under the condition of the free boundary of the whole satellite, determining the local structure frequency of the reinforced momentum wheel according to the formant occurrence frequency, and determining whether the in-orbit frequency avoidance reinforcement design meets the requirement or not.

The invention discloses a method for detecting tert-butylamine residues in S-acetamidomethyl-L-cysteine-L-leucine tert-butyl ester, and mainly solves the problem that the existing method for detecting the tert-butylamine residues in the S-acetamidomethyl-L-cysteine-L-leucine tert-butyl ester comprises the following steps: 1, preparing a sample, 2, carrying out gas chromatography detection, and 3, detecting the tert-butylamine residues in the S-acetamidomethyl-L-cysteine-L-leucine tert-butyl ester. According to the method, a polyethylene glycol capillary chromatographic column is mainly utilized, a gas chromatograph hydrogen flame detector is combined, and the tert-butylamine residue in the S-acetamidomethyl-L-cysteine-L-leucine tert-butyl ester is calculated according to a standard curve method.According to the method, synchronous detection of a to-be-detected sample without interference is achieved, the measurement precision is high, the method is simple, rapid and good in repeatability, and the method is suitable for large-scale popularization and application of the tert-butylamine residue in the S-acetamidomethyl-L-cysteine-L-leucine tert-butyl ester. And a reliable and convenient detection means is provided for detecting the tert-butylamine residue in the S-acetamidomethyl-L-cysteine-L-leucine tert-butyl ester.

The invention provides a molecular marker S603 related to the glycogen content character of ostrea rivularis as well as primers and application of the molecular marker S603, and belongs to the technical field of molecular marker breeding. The nucleotide sequence of the molecular marker S603 is as shown in SEQ ID No.1, and the basic group genotype AT at the 689th site of the molecular marker S603 is a genotype with high glycogen content. The invention also provides a primer for detecting the molecular marker S603, the nucleotide sequences of an amplification primer are shown as SEQ ID No.2 and SEQ ID No.3, and the sequence of an extension primer is shown as SEQ ID No.4. The molecular marker S603 can accelerate the breeding speed of the high-glycogen-content character variety of the ostrea rivularis, a large amount of manpower and material resources are saved, the progress of improved variety breeding is promoted, healthy and sustainable development of the ostrea rivularis industry is facilitated, and the molecular marker S603 has a wide market application prospect.

The application discloses a pumped storage full-power frequency converter power unit variable frequency control method and device, N power units are cascaded at an AC end to form a test valve, and M power units are cascaded at the AC end to form a test valve; the method is used for controlling the test valve to output several AC frequency target values, and the method comprises the following steps: pre-charging DC capacitors in the test valve and the test valve to target values; determining corresponding output current / power target values according to the AC frequency target values to be output; controlling the turn-on and turn-off time of the turn-off semiconductor of each power unit in the test valve and the test valve, so that the actual output current / power of the test valve is the output current / power target value, and the actual output frequency of the test valve is the AC frequency target value. The application can simulate the continuous speed regulation operation condition of the pumped storage full-power frequency converter power unit on the pumped storage unit.

This invention discloses a method for determining the upper limit of water saturation in tight gas reservoirs using two-phase permeability analysis, comprising: 1. Selecting several rock samples for gas-water two-phase permeability analysis, and establishing a gas-water phase permeability curve based on the relative permeability of the water phase, the relative permeability of the gas phase, and the water saturation value of each rock sample; 2. Based on the gas-water phase permeability curve, first reading the water saturation value of each rock sample when the relative permeability of the water phase is a set value A, then reading the relative permeability of the gas phase corresponding to the same water saturation value of each rock sample, and determining the upper limit of water saturation for each rock sample accordingly; 3. Calculating the average value of the upper limit of water saturation for each rock sample, and the obtained average value is the upper limit of water saturation for the tight gas reservoir. This invention can accurately determine the upper limit of water saturation in tight gas reservoirs using relatively simple means, simplifying the determination process and improving accuracy compared to existing technologies, providing an accurate basis for the rational exploitation of tight gas reservoirs.

This invention discloses the application of a reagent that inhibits ADNP gene expression or ADNP protein activity in the preparation of drugs for treating renal fibrosis, belonging to the field of biomedical technology. The application of the reagent that inhibits ADNP gene expression or ADNP protein activity in the preparation of drugs for treating renal fibrosis. This invention effectively knocks down or knocks out the ADNP gene in renal tubular epithelial cells, objectively investigates the effects of ADNP deficiency on a series of phenotypes in HK-2 cells, and confirms that ADNP can inhibit EMT and renal fibrosis in HK-2 cells. Drugs that target and knock down or knock out the ADNP gene in human kidneys can be developed to delay the progression of renal fibrosis.

The present invention provides a molecular marker 23W4 and its application in screening a population of Penaeus vannamei with high WSSV resistance. The nucleotide sequence of the molecular marker 23W4 is shown in SEQ ID No.1, and the genotype AA of the 301st site of the molecular marker 23W4 is a WSSV resistance genotype. The present invention also provides primers for the molecular marker 23W4, including amplification primers with nucleotide sequences shown in SEQ ID No.2 and SEQ ID No.3 and an extension primer with a nucleotide sequence shown in SEQ ID No.4. The molecular marker 23W4 with high WSSV resistance of Penaeus vannamei is not limited by the growth stage, can be used for the early breeding of Penaeus vannamei, screen WSSV-resistant individuals in breeding populations with different growth performances, quickly breed parent populations with high WSSV resistance, and promote the breeding process of new varieties of Penaeus vannamei with high WSSV resistance.

The invention provides a welded wing rail reinforced alloy steel combined frog and a preparation method thereof. The welded wing rail reinforced alloy steel combined frog comprises a point rail and wing rails arranged on the two sides of the point rail. The wing rail comprises a front section steel rail, a middle section steel rail and a rear section steel rail which are fixedly connected in sequence from the toe end to the heel end of the frog; the front-section steel rail and the rear-section steel rail are respectively made of carbon steel or low-alloy steel, and the middle-section steel rail is made of middle-alloy steel or high-alloy steel; a first connecting area is arranged between the front-section steel rail and the middle-section steel rail, and the first connecting area is located within the range of 300-400 mm in the direction from the throat of the wing rail to the toe end of the frog; a second connecting area is arranged between the middle section steel rail and the rear section steel rail, and the second connecting area is located at the position, corresponding to the wing rail, within the range of 60-100 mm of the section width of the point rail. According to the welded wing rail reinforced alloy steel combined frog and the preparation method thereof, the performance of the wing rail can be effectively improved, the abrasion speed of the frog is reduced, and the safety and reliability of wheel rail operation are improved.

The invention discloses a force-heat coupling verification test method suitable for a thruster support, and the method comprises the steps: determining test piece parameters according to a product design state and a verification purpose; determining test loads including a force load and a temperature load according to force and thermal simulation results and a test purpose; designing a test fixing clamp; determining a loading mode and a control mode of the force load and the temperature load according to the test load; a thruster support is fixed on a test fixing clamp, and according to a loading mode and a control mode of a load, a force-heat coupling loading method is adopted to carry out force-heat coupling verification. The method has the advantages of flexible application of coupling and decoupling working conditions, simple operation, wide application range, accurate and reliable result and the like.