The invention discloses a cast-in-place prestressed concrete continuous box girder one-time pouring and forming construction method. The method includes the steps of foundation treatment, support erection, bottom mold and side mold laying, support pre-pressing, bottom mold and side mold adjustment, arrangement of bottom boards, webs and crossbeam reinforcing steel bars, prestressed pipelines and prestressed ribs, inner mold and end mold laying, arrangement of top board reinforcing steel bars, prestressed pipelines and prestressed ribs, top board reinforcing steel bar installation, concrete pouring and curing, prestressed rib tensioning, mud jacking and sealing anchorage, and formwork and support detachment. The problems of construction joints generated by a traditional construction processand transverse cracks of the jointing positions of flange plates and the webs can be solved, and the integral rigidity of a girder body is enhanced; the construction period of a continuous box girder is shortened, and the schedule of construction is quickened; and the use rate of a box girder formwork and steel pipe support transfer materials is increased, work efficiency is improved, treatmentof the construction joints is cancelled, erection and detachment work of supports in a box is reduced, working procedures are effectively switched, and expenditure of project management cost is reduced.