The invention provides effective technology for extracting active ingredients of Entermorpha and a method for preparing an Entermorpha alga fertilizer. The method is characterized by comprising: taking the Entermorpha as a raw material, effectively extracting the active ingredients of the Entermorpha through technical processes such as pretreatment of raw materials, leaching, neutralization, concentration and combination, taking an extracting solution as a base fluid, combining the extracting solution with at least one composition of humic acid, urea, potassium dihydrogen phosphate, potassiumsulfate, monoammonium phosphate and microelement, and preparing the high-activity Entermorpha alga fertilizer. The Entermorpha alga fertilizer is rich in active substances of algae, not only can strengthen photosynthesis of crops, increase the yield of the crops and improve the product quality but also can strengthen the cold resistance, the drought resistance, the stress resistance and the disease resistance of the crops, promote earliness of the crops and increase the fertile flower percentage and the fertile fruit percentage, has lower production cost, good solubility and safe use, is friendly and harmless to human beings, domestic animals and the natural environment, and is a novel environment-friendly fertilizer suitable for modern agriculture.