The invention discloses a detection kit, detection
system and detection method for 19 high-risk HPVs. The 19 high-risk HPVs comprise HPV16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 70, 73 and 82. According to the invention, a plurality of general primers are employed for amplification of target fragments, and
specific fluorescence probes are used for real-time detection of the amplified fragments; since the general primers are employed, the primer amount of the
system and complexity of the
system are reduced, cost is saved, and all the high risk types are detected through combined action of 10 primers; and 20
specific fluorescence probes are used to distinguish different types. With the detection kit, detection system and detection method provided by the invention, the 19 high-risk HPVs can be detected in a
reaction tube; high sensitivity and good specificity are obtained; operation is simple and fast; and for patients, screening cost is saved.