This utility model relates to an integrated biological experimental plate loading and unloading chamber, comprising a frame, a chamber door, a tray, and a sliding mechanism. The chamber door is rotatably mounted on the frame via a spring-loaded mechanism; the tray is movably mounted on the frame, and a drive device is provided on the frame, connecting the drive device to the tray; the sliding mechanism is mounted on the tray, enabling relative sliding between the tray and the chamber door. The integrated biological experimental plate loading and unloading chamber, through the spring-loaded mechanism and the sliding mechanism, achieves automatic opening and closing of the chamber door during tray loading and unloading, eliminating the need for manual opening and closing by personnel, greatly reducing manual intervention, improving work efficiency, avoiding the influence of human hands on the experimental environment, and ensuring experimental accuracy.