The invention discloses a hospital human resource quantitative configuration method based on a queuing theory model. The configuration method of the queuing theory model comprises four basic structures: an input procedure, service time, a service window and a queuing rule; and the method mainly comprises the following steps: step one, performing workload statistics and questionnaire survey, wherein the statistics index is the working hours and total medical consultations, and the questionnaire survey content is the medical staff acceptable maximum working strength and patient bearable longest waiting time; step two, measuring basic parameters by use of a work-hour measurement method: a service counter number C, an average arrival rate lambda and an average service rate mu; step three, establishing the queuing theory model, analyzing the data by use of the queuing theory analysis model; step four, outputting the following outcome indexes: the working strength rho, an average waiting number Lq, an average staying number Ls, average waiting time Wq, average remaining time Ws, an idle probability Po and a waiting probability P; comparing various data indexes, and regarding the service counter number C satisifying that the working strength rho is less than one and meets the medical staff working strength and the patient waiting time as an optimal data result. The method disclosed by the invention has the advantages that the quantitative configuration problem of the medical staff is effectively solved, and the scientific reference is provided for improving the service effect.