The invention relates to the field of medical equipment, and particularly discloses an intelligent number taking and calling system which comprises a main control box, a self-service card taking machine, a plurality of number calling units and a plurality of blocking units; each number calling unit comprises a number calling device and a display screen, and the self-service card taking machine, the number calling devices and the display screens are electrically connected with the main control box; each blocking unit comprises a gate, an entrance gate and a plurality of fences each for blockingsingle blood drawing inspection window, the entrance gate is fixedly arranged between the fences, a scanning port is formed in the entrance gate, a first ticket reading module is arranged in the entrance gate, the first ticket reading module is opposite to the scanning port, the first ticket reading module controls the gate to be opened or closed, and the first ticket reading module is electrically connected with the main control box; an outlet is formed in the side, close to a blood drawing inspection window, of the fences and rotationally provided with a stop lever. According to the intelligent number taking and calling system, through cooperation of the fences, the gates and the stop lever, a patient waiting for blood sampling can be far away from the blood drawing inspection window, then the blood sampling order at the blood drawing inspection window can be guaranteed, and the blood sampling efficiency of doctors or nurses is improved.